SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing BWI76_RS24870 FitnessBrowser__Koxy:BWI76_RS24870 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 14 hits to proteins with known functional sites (download)

1j6tA Complex of enzyme iiamtl and the histidine-containing phosphocarrier protein hpr from escherichia coli nmr, restrained regularized mean structure (see paper)
42% identity, 52% coverage: 1:140/271 of query aligns to 2:143/144 of 1j6tA

query
sites
1j6tA

M

F

Q

K

L

L

S

G

C

A

A

E

D

N

I

I

H

F

F

L

D

G

R

R

E

K

I

A

K

A

T

T

K

K

D

E

E

E

A

A

L

I

Q

R

R

F

V

A

V

G

G

E

R

Q

L

L

T

V

E

K

A

G

G

G

H

Y

T

V

T

E

A

P

A

E

Y

Y

L

V

N

Q

G

A

M

M

R

L

D

D

R
|
R

E

E

G

K

Q

L

I

T

S

P

T

T

Y

Y

L

L

G

G

N

E

G

S

I

I

A

A

I

V

P

P

H
|
H

G

G

T

T

P

V

Q

E

S

A

R

K

D

D

A

R

V

V

L

L

K

K

T

T

G

G

V

V

K

V

V

F

L

C

A

Q

C

Y

P

P

Q

E

G

G

V

V

D

R

W

F

G

G

E

E

Q

E

-

D

T

I

A

A

Y

R

L

L

I

V

V

I

G

G

I

I

A

A

Q

R

D

N

S

N

E

E

H
|
H

L

I

D

Q

I

V

L

I

R

T

Q

S

L

L

T

T

H

N

A

A

M

L

S

D

D

D

E

E

R

S

V

V

P

I

E

E

A

R

L

L

S

A

R

H

T

T

E

T

S

S

P

V

Q

D

A

E

V

V

L

L

E

E

I

L

L

L

A

A

G

G

binding phosphite ion: R46 (= R45), H62 (= H61), H108 (= H105)

P00550 PTS system mannitol-specific EIICBA component; EIICBA-Mtl; EII-Mtl; EC 2.7.1.197 from Escherichia coli (strain K12) (see 2 papers)
42% identity, 52% coverage: 1:140/271 of query aligns to 494:635/637 of P00550

query
sites
P00550

M

F

Q

K

L

L

S

G

C

A

A

E

D

N

I

I

H

F

F

L

D

G

R

R

E

K

I

A

K

A

T

T

K

K

D

E

E

E

A

A

L

I

Q

R

R

F

V

A

V

G

G

E

R

Q

L

L

T

V

E

K

A

G

G

G

H

Y

T

V

T

E

A

P

A

E

Y

Y

L

V

N

Q

G

A

M

M

R

L

D

D

R
|
R

E

E

G

K

Q

L

I

T

S

P

T

T

Y

Y

L

L

G

G

N

E

G

S

I

I

A

A

I

V

P

P

H
|
H

G

G

T

T

P

V

Q

E

S

A

R

K

D

D

A

R

V

V

L

L

K

K

T

T

G

G

V

V

K

V

V

F

L

C

A

Q

C

Y

P

P

Q

E

G

G

V

V

D

R

W

F

G

G

E

E

Q

E

-

D

T

I

A

A

Y

R

L

L

I

V

V

I

G

G

I

I

A

A

Q

R

D

N

S

N

E

E

H

H

L

I

D

Q

I

V

L

I

R

T

Q

S

L

L

T

T

H

N

A

A

M

L

S

D

D

D

E

E

R

S

V

V

P

I

E

E

A

R

L

L

S

A

R

H

T

T

E

T

S

S

P

V

Q

D

A

E

V

V

L

L

E

E

I

L

L

L

A

A

G

G

R538 (= R45) Stabilizes the transition state in the phosphoryl transfer from HPr to EIIA
H554 (= H61) modified: Phosphohistidine; by HPr

Sites not aligning to the query:

384 modified: Phosphocysteine; by EIIA

C0H3V2 Mannitol-specific phosphotransferase enzyme IIA component; EIIA; EIII; PTS system mannitol-specific EIIA component from Bacillus subtilis (strain 168) (see paper)
35% identity, 50% coverage: 3:138/271 of query aligns to 4:139/143 of C0H3V2

query
sites
C0H3V2

L

L

S

A

C

K

A

E

D

N

I

I

H

K

F

L

D

N

R

Q

E

T

I

V

K

S

T

S

K

K

D

E

E

E

A

A

L

I

Q

K

R

L

V

A

V

G

G

Q

R

T

L

L

T

I

E

D

A

N

G

G

H

Y

T

V

T

T

A

E

A

D

Y

Y

L

I

N

S

G

K

M

M

R

F

D

E

R

R

E

E

G

E

Q

T

I

S

S

S

T

T

Y

F

L

M

G

G

N

N

G

F

I

I

A

A

I

I

P

P

H

H

G

G

T

T

P

E

Q

E

S

A

R

K

D

S

A

E

V

V

L

L

K

H

T
x
S

G

G

V

I

K

S

V

I

L

I

A

Q

C

I

P

P

Q

E

G

G

V

V

D

E

W

Y

G

G

E

E

Q

G

Q

N

T

T

A

A

Y

K

L

V

I

V

V

F

G

G

I

I

A

A

A

G

Q

K

D

N

S

N

E

E

H

H

L

L

D

D

I

I

L

L

R

S

Q

N

L

I

T

A

H

I

A

I

M

C

S

S

D

E

E

E

R

E

V

N

P

I

E

E

A

R

L

L

S

I

R

S

T

A

E

K

S

S

P

E

Q

E

A

D

V

L

L

I

E

A

I

I

L

F

S74 (≠ T73) modified: Phosphoserine

Q9F166 Phosphocarrier protein HPr from Bacillus thuringiensis subsp. israelensis (see 2 papers)
40% identity, 32% coverage: 159:246/271 of query aligns to 2:87/87 of Q9F166

query
sites
Q9F166

E

E

A

K

T

I

F

F

T

K

L

V

R

T

N

S

P

D

H

S

G

G

L

I

H
|
H

A

A

R

R

P

P

S

A

A

T

V

L

L

L

V

V

K

N

A

T

V

A

K

S

Q

K

W

F

R

G

S

S

Q

D

I

I

R

-

V

-

E

-

N

N

L

L

D

E

T

Y

R

N

S

G

A

K

I

N

V

V

D

N

A

L

K

K

N
x
S

L

I

M

M

R

G

V

V

V

M

S

S

L

L

G

G

A

I

K

Q

Q

Q

G

N

H

A

R

E

L

I

H

K

F

I

M

T

A

A

S

N

G

G

E

D

D

D

A

A

H

A

Q

Q

A

A

L

L

E

A

A

A

I

I

-

E

G

E

T

T

A

M

F

K

N

N

A

E

G

G

L

L

G

G

E

E

H14 (= H171) modified: Tele-phosphohistidine; alternate; mutation to A: Not histidine-phosphorylated.
S45 (≠ N205) modified: Phosphoserine; by HPrK/P; mutation to A: Not serine-phosphorylated. Absence of glucose-induced cry4A expression repression. 60 to 70% reduction in the sporulation efficiency.

O69250 Phosphocarrier protein HPr; Histidine-containing protein from Priestia megaterium (Bacillus megaterium) (see 2 papers)
39% identity, 32% coverage: 161:246/271 of query aligns to 5:88/88 of O69250

query
sites
O69250

T

T

F

F

T

T

L

V

R

T

N

A

P

D

H

S

G

G

L

I

H

H

A

A

R

R

P

P

S

A

A

T

V

T

L

L

V

V

K

Q

A

A

V

A

K

S

Q

K

W

F

R

D

S

S

Q

D

I

I

R

-

V

-

E

-

N

N

L

L

D

E

T

F

R

N

S

G

A

K

I

T

V

V

D

N

A

L

K

K

N
x
S

L

I

M

M

R

G

V

V

V

M

S

S

L

L

G

G

A

I

K

Q

Q

K

G

G

H

A

R

T

L

I

H

T

F

I

M

S

A

A

S

E

G

G

E

S

D

D

A

E

H

A

Q

D

A

A

L

L

E

A

A

A

I

L

-

E

G

D

T

T

A

M

F

S

N

K

A

E

G

G

L

L

G

G

E

E

S46 (≠ N205) modified: Phosphoserine; by HPrK/P

1pohA The 2.0 angstroms resolution structure of escherichia coli histidine- containing phosphocarrier protein hpr: a redetermination (see paper)
40% identity, 30% coverage: 155:234/271 of query aligns to 1:75/85 of 1pohA

query
sites
1pohA

V

M

F

F

D

Q

E

Q

E

E

A

V

T

T

F

I

T

T

L

-

R

-

N

A

P

P

H

N

G

G

L

L

H

H

A

T

R

R

P

P

S

A

A

A

V

Q

L

F

V

V

K

K

A

E

V

A

K

K

Q

G

W

F

R

T

S

S

Q

E

I

I

R

T

V

V

E

T

N

S

L

-

D
x
N

T

G

R
x
K

S

S

A

A

I

-

V

-

D

S

A

A

K

K

N

S

L

L

M

F

R

K

V

L

V

Q

S

T

L

L

G

G

A

L

K

T

Q

Q

G

G

H

T

R

V

L

V

H

T

F

I

M

S

A

A

S

E

G

G

E

E

D

D

A

E

H

Q

Q

K

A

A

L

V

E

E

binding sulfate ion: N38 (≠ D195), K40 (≠ R197)

1j6tB Complex of enzyme iiamtl and the histidine-containing phosphocarrier protein hpr from escherichia coli nmr, restrained regularized mean structure (see paper)
40% identity, 30% coverage: 155:234/271 of query aligns to 1:75/85 of 1j6tB

query
sites
1j6tB

V

M

F

F

D

Q

E

Q

E

E

A

V

T

T

F

I

T

T

L

-

R

-

N

A

P

P

H

N

G

G

L

L

H
|
H

A
x
T

R

R

P

P

S

A

A

A

V

Q

L

F

V

V

K

K

A

E

V

A

K

K

Q

G

W

F

R

T

S

S

Q

E

I

I

R

T

V

V

E

T

N

S

L

-

D

N

T

G

R

K

S

S

A

A

I

-

V

-

D

S

A

A

K

K

N

S

L

L

M

F

R

K

V

L

V

Q

S

T

L

L

G

G

A

L

K

T

Q

Q

G

G

H

T

R

V

L

V

H

T

F

I

M

S

A

A

S

E

G

G

E

E

D

D

A

E

H

Q

Q

K

A

A

L

V

E

E

binding phosphite ion: H15 (= H171), T16 (≠ A172)

P0AA04 Phosphocarrier protein HPr; Histidine-containing protein from Escherichia coli (strain K12) (see paper)
40% identity, 30% coverage: 155:234/271 of query aligns to 1:75/85 of P0AA04

query
sites
P0AA04

V

M

F

F

D

Q

E

Q

E

E

A

V

T

T

F

I

T

T

L

-

R

-

N

A

P

P

H

N

G

G

L

L

H
|
H

A

T

R

R

P

P

S

A

A

A

V

Q

L

F

V

V

K

K

A

E

V

A

K

K

Q

G

W

F

R

T

S

S

Q

E

I

I

R

T

V

V

E

T

N

S

L

-

D

N

T

G

R

K

S

S

A

A

I

-

V

-

D

S

A

A

K

K

N

S

L

L

M

F

R

K

V

L

V

Q

S

T

L

L

G

G

A

L

K

T

Q

Q

G

G

H

T

R

V

L

V

H

T

F

I

M

S

A

A

S

E

G

G

E

E

D

D

A

E

H

Q

Q

K

A

A

L

V

E

E

H15 (= H171) active site, Pros-phosphohistidine intermediate

P08877 Phosphocarrier protein HPr; Histidine-containing protein from Bacillus subtilis (strain 168) (see 5 papers)
36% identity, 32% coverage: 159:246/271 of query aligns to 3:88/88 of P08877

query
sites
P08877

E

Q

A

K

T

T

F

F

T

K

L

V

R

T

N

A

P

D

H
x
S

G

G

L

I

H
|
H

A

A

R

R

P

P

S

A

A

T

V

V

L

L

V

V

K

Q

A

T

V

A

K

S

Q

K

W

Y

R

D

S

A

Q

D

I

V

R

-

V

-

E

-

N

N

L

L

D

E

T

Y

R

N

S

G

A

K

I

T

V

V

D

N

A

L

K

K

N
x
S

L

I

M

M

R

G

V

V

V

M

S

S

L

L

G

G

A

I

K

A

Q

K

G

G

H

A

R

E

L

I

H

T

F

I

M

S

A

A

S

S

G

G

E

A

D

D

A

E

H

N

Q

D

A

A

L

L

E

N

A

A

I

L

-

E

G

E

T

T

A

M

F

K

N

S

A

E

G

G

L

L

G

G

E

E

S12 (≠ H168) modified: Phosphoserine
H15 (= H171) active site, Pros-phosphohistidine intermediate; alternate; modified: Tele-phosphohistidine; alternate
S46 (≠ N205) modified: Phosphoserine; by HPrK/P; mutation to A: No phosphorylation by HPrK/P; abolishes the catabolite repressive effects of glucose on gluconate kinase, glucitol dehydrogenase, and the mannitol-specific catabolic enzymes.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

Q84F84 Phosphocarrier protein HPr; Histidine-containing protein from Lysinibacillus sphaericus (Bacillus sphaericus) (see paper)
36% identity, 28% coverage: 162:238/271 of query aligns to 6:79/88 of Q84F84

query
sites
Q84F84

F

F

T

T

L

V

R

I

N

D

P

P

H

L

G

G

L

I

H
|
H

A

A

R

R

P

P

S

A

A

S

V

Q

L

L

V

V

K

A

A

K

V

A

K

T

Q

P

W

F

R

A

S

S

Q

A

I

I

R

E

V

V

E

R

N

-

L

-

D

-

T

T

R

E

S

E

A

K

I

A

V

A

D

N

A

L

K

K

N
x
S

L

I

M

L

R

G

V

V

V

M

S

G

L

L

G

A

A

L

K

K

Q

Q

G

G

H

S

R

Q

L

F

H

T

F

L

M

Y

A

V

S

E

G

G

E

E

D

D

A

E

H

D

Q

Q

A

A

L

F

E

E

A

A

I

L

G

A

T

T

H15 (= H171) active site, Pros-phosphohistidine intermediate
S46 (≠ N205) modified: Phosphoserine; by HPrK/P; mutation to Q: No phosphorylation by HPrK/P.

P24366 Phosphocarrier protein HPr; Histidine-containing protein from Streptococcus salivarius (see paper)
37% identity, 28% coverage: 162:236/271 of query aligns to 6:77/87 of P24366

query
sites
P24366

F

F

T

H

L

I

R

V

N

A

P

E

H

T

G

G

L

I

H

H

A

A

R

R

P

P

S

A

A

T

V

L

L

L

V

V

K

Q

A

T

V

A

K

S

Q

K

W

F

R

A

S

S

Q

D

I

I

R

-

V

-

E

-

N

T

L

L

D

D

T

Y

R

K

S

G

A

K

I

A

V

V

D

N

A

L

K

K

N
x
S

L

I

M

M

R

G

V

V

V

M

S

S

L

L

G

G

A

V

K

G

Q

Q

G

G

H

A

R

D

L

V

H

T

F

I

M

S

A

A

S

E

G

G

E

A

D

D

A

A

H

D

Q

D

A

A

L

I

E

V

A

A

I

I

S46 (≠ N205) modified: Phosphoserine; by HPrK/P

O31645 PTS system mannose-specific EIIBCA component; EIIBCA-Man; EII-Man; EC 2.7.1.191 from Bacillus subtilis (strain 168) (see 2 papers)
27% identity, 46% coverage: 15:138/271 of query aligns to 518:646/650 of O31645

query
sites
O31645

K

E

T

T

K

S

D

D

E

D

A

I

L

I

Q

D

R

E

V

L

V

I

G

Q

R

K

L

L

T

S

E

R

A

R

G

G

H

A

-

L

T

S

T

E

A

S

A

G

Y

F

L

K

N

Q

G

A

M

I

R

L

D

N

R

R

E

E

G

Q

Q

Q

I

G

S

T

T

T

Y

A

L

I

G

G

N

M

G

N

I

I

A

A

I

I

P

P

H

H

G

G

T

K

P

S

Q

E

S

A

-

V

R

R

D

E

A

P

V

S

L

V

K

A

T

F

G

G

V

I

K

K

V

-

L

-

A

-

C

-

P

R

Q

S

G

G

V

V

D

D

W

W

G

N

-

S

-

L

E

D

Q

G

Q

S

T

E

A

A

Y

K

L

L

I

I

V

F

G

M

I

I

A

A

-

V

-

P

-

K

-

E

A

S

Q

G

D

G

S

N

E

Q

H

H

L

L

D

K

I

I

L

L

R

Q

Q

M

L

L

T

S

H

R

A

K

M

L

S

M

D

D

E

D

R

N

V

Y

P

R

E

E

A

R

L

L

S

L

R

S

T

V

E

Q

S

T

P

T

Q

E

A

E

V

A

L

Y

E

K

I

L

L

L

Sites not aligning to the query:

9 C→A: Appears to be unable to inactivate ManR, since ManR does not show any decrease in its DNA-binding activity in the presence of this mutant.
365 modified: Phosphoserine

Q9CJ83 Phosphocarrier protein HPr; Histidine-containing protein from Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis) (see paper)
37% identity, 29% coverage: 169:246/271 of query aligns to 13:88/88 of Q9CJ83

query
sites
Q9CJ83

G

G

L

I

H

H

A

A

R

R

P

P

S

A

A

T

V

L

L

L

V

V

K

H

A

T

V

A

K

S

Q

K

W

F

R

T

S

S

Q

E

I

I

R

-

V

-

E

-

N

T

L

L

D

E

T

Y

R

K

S

G

A

K

I

S

V

V

D

N

A

L

K

K

N
x
S

L

I

M

M

R

G

V

V

V

M

S

S

L

L

G

G

A

V

K

G

Q

Q

G

G

H

A

R

D

L

V

H

T

F

I

M

S

A

A

S

E

G

G

E

A

D

D

A

A

H

D

Q

D

A

A

L

I

E

S

A

T

I

I

G

A

-

E

T

T

A

M

F

T

N

K

A

E

G

G

L

L

G

A

E

E

S46 (≠ N205) modified: Phosphoserine; by HPrK/P; mutation to A: Abolished S-46 phosphorylation.

Q9WXK8 Phosphocarrier protein HPr; Histidine-containing protein from Streptococcus equinus (Streptococcus bovis) (see paper)
35% identity, 24% coverage: 162:226/271 of query aligns to 6:67/87 of Q9WXK8

query
sites
Q9WXK8

F

F

T

H

L

I

R

V

N

A

P

E

H

T

G

G

L

I

H

H

A

A

R

R

P

P

S

A

A

T

V

L

L

L

V

V

K

Q

A

T

V

A

K

S

Q

K

W

F

R

A

S

S

Q

D

I

I

R

-

V

-

E

-

N

T

L

L

D

D

T

Y

R

K

S

G

A

K

I

A

V

V

D

N

A

L

K

K

N
x
S

L

I

M

M

R

G

V

V

V

M

S

S

L

L

G

G

A

V

K

G

Q

Q

G

G

H

A

R

D

L

V

H

T

F

I

M

S

A

A

S

E

G

G

S46 (≠ N205) mutation to A: No phosphorylation by HPrK/P.

Query Sequence

>BWI76_RS24870 FitnessBrowser__Koxy:BWI76_RS24870
MQLSCADIHFDREIKTKDEALQRVVGRLTEAGHTTAAYLNGMRDREGQISTYLGNGIAIP
HGTPQSRDAVLKTGVKVLACPQGVDWGEQQTAYLIVGIAAQDSEHLDILRQLTHAMSDER
VPEALSRTESPQAVLEILAGNIFPAEGEDMEPPPVFDEEATFTLRNPHGLHARPSAVLVK
AVKQWRSQIRVENLDTRSAIVDAKNLMRVVSLGAKQGHRLHFMASGEDAHQALEAIGTAF
NAGLGEIAAQPQQVVQPAEKPKRSWLSRIFS

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory