Comparing BWI76_RS27165 BWI76_RS27165 mannitol-1-phosphate 5-dehydrogenase to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 12 hits to proteins with known functional sites (download)
P09424 Mannitol-1-phosphate 5-dehydrogenase; EC 1.1.1.17 from Escherichia coli (strain K12) (see paper)
90% identity, 100% coverage: 1:382/382 of query aligns to 1:382/382 of P09424
Q4X1A4 Mannitol-1-phosphate 5-dehydrogenase; M1PDH; MPD; MPDH; EC 1.1.1.17 from Aspergillus fumigatus (strain ATCC MYA-4609 / CBS 101355 / FGSC A1100 / Af293) (Neosartorya fumigata) (see paper)
40% identity, 95% coverage: 2:365/382 of query aligns to 4:367/388 of Q4X1A4
4im7A Crystal structure of fructuronate reductase (ydfi) from e. Coli cft073 (efi target efi-506389) complexed with nadh and d-mannonate
29% identity, 64% coverage: 99:341/382 of query aligns to 159:409/483 of 4im7A
Sites not aligning to the query:
7rk5B Mannitol-2-dehydrogenase bound to nadh from aspergillus fumigatus
24% identity, 61% coverage: 102:334/382 of query aligns to 180:422/501 of 7rk5B
Sites not aligning to the query:
1m2wA Pseudomonas fluorescens mannitol 2-dehydrogenase ternary complex with NAD and d-mannitol (see paper)
26% identity, 51% coverage: 99:293/382 of query aligns to 169:374/492 of 1m2wA
Sites not aligning to the query:
1lj8A Crystal structure of mannitol dehydrogenase in complex with NAD (see paper)
26% identity, 51% coverage: 99:293/382 of query aligns to 169:374/492 of 1lj8A
Sites not aligning to the query:
7ocqA Nadh bound to the dehydrogenase domain of the bifunctional mannitol-1- phosphate dehydrogenase/phosphatase mtld from acinetobacter baumannii (see paper)
23% identity, 88% coverage: 7:344/382 of query aligns to 250:654/686 of 7ocqA
Sites not aligning to the query:
7ocsA Mannitol-1-phosphate bound to the phosphatase domain of the bifunctional mannitol-1-phosphate dehydrogenase/phosphatase mtld-d16a from acinetobacter baumannii (see paper)
23% identity, 88% coverage: 7:344/382 of query aligns to 250:650/682 of 7ocsA
Sites not aligning to the query:
7ocqB Nadh bound to the dehydrogenase domain of the bifunctional mannitol-1- phosphate dehydrogenase/phosphatase mtld from acinetobacter baumannii (see paper)
23% identity, 88% coverage: 7:344/382 of query aligns to 245:647/679 of 7ocqB
Sites not aligning to the query:
7ocrB NADPH and fructose-6-phosphate bound to the dehydrogenase domain of the bifunctional mannitol-1-phosphate dehydrogenase/phosphatase mtld from acinetobacter baumannii (see paper)
23% identity, 88% coverage: 7:344/382 of query aligns to 244:643/675 of 7ocrB
Sites not aligning to the query:
7ocnA Crystal structure of the bifunctional mannitol-1-phosphate dehydrogenase/phosphatase mtld from acinetobacter baumannii (see paper)
30% identity, 34% coverage: 217:344/382 of query aligns to 528:658/690 of 7ocnA
Sites not aligning to the query:
7ocpA NADPH bound to the dehydrogenase domain of the bifunctional mannitol- 1-phosphate dehydrogenase/phosphatase mtld from acinetobacter baumannii (see paper)
30% identity, 34% coverage: 217:344/382 of query aligns to 528:658/688 of 7ocpA
Sites not aligning to the query:
>BWI76_RS27165 BWI76_RS27165 mannitol-1-phosphate 5-dehydrogenase
MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARHSYQVHVVGENEQVDTV
SGVDAVSSIGDEVVELIASVDLVTTAVGPVVLERIAPAIAKGLAKRKAQGVTAPLNIIAC
ENMVRGTSQLKGHVFNALAEEDKAWVETNVGFVDSAVDRIVPPSASATHDPLEVTVETFS
EWIVDKTQFKGELPDIPGMELTDNLMAFVERKLFTLNTGHAITAYLGKLAGHQTIRDAIL
DENIRAVVKGAMEESGAVLIKRYAFDAQKHAAYIQKIIGRFENPYLKDDVERVGRQPLRK
LSAGDRLIKPLLGTLEYGLPHRNLVKGIAAAMHFRSEEDPQAQELAALIAEKGPQAALAQ
ISGLDAASEVVAEAVNDYSATK
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SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.
Lawrence Berkeley National Laboratory