SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing BWI76_RS27745 FitnessBrowser__Koxy:BWI76_RS27745 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 3 hits to proteins with known functional sites (download)

P46318 Lichenan-specific phosphotransferase enzyme IIB component; EIIB-Lic; PTS system lichenan-specific EIIB component; EC 2.7.1.- from Bacillus subtilis (strain 168) (see paper)
43% identity, 93% coverage: 4:99/103 of query aligns to 3:98/102 of P46318

query
sites
P46318

I

I

M

L

L

L

C

V

C

C

S

A

A

A

G

G

M

M

S

S

T

T

S

S

L

L

V

V

R

S

K

K

M

M

V

E

D

K

A

S

A

A

K

Q

E

E

R

Q

N

G

L

K

P

D

V

Y

Q

T

I

I

D

W

A

A

Y

V

G
x
S

V

G

S

D

E

S

F

V

D

Q

M

N

Q

H

F

I

P

D

Q

K

Y

A

Q

D

V

V

V

L

L

L

G

G

P

P

Q

Q

V

V

K

R

Y

Y

M

M

L

L

K

P

N

Q

L

L

S

K

E

K

K

L

A

G

A

E

P

S

L

K

N

G

I

V

P

P

V

V

Q

D

P

V

I

I

N

N

T

T

M

V

D

H

Y

Y

G

G

M

T

Q

C

R

N

G

G

D

A

N

E

V

V

L

L

D

K

Y

S

A

A

L

E

S

Q

L

L

S37 (≠ G38) modified: Phosphoserine

2wy2D Nmr structure of the iiachitobiose-iibchitobiose phosphoryl transition state complex of the n,n'-diacetylchitoboise brance of the e. Coli phosphotransferase system. (see paper)
44% identity, 87% coverage: 4:93/103 of query aligns to 4:91/103 of 2wy2D

query
sites
2wy2D

I

I

M

Y

L

L

C

F

C
|
C

S
|
S

A
|
A

G
|
G

M
|
M

S
|
S

T

T

S

S

L

L

V

V

R

S

K

K

M

M

V

R

D

A

A

Q

A

A

K

E

E

K

R

Y

N

E

L

V

P

P

V

V

Q

I

I

I

D

E

A

A

Y

F

G

P

V

E

S

T

E

L

F

A

D

G

M

E

Q

K

F

G

P

Q

Q

N

Y

A

Q

D

V

V

L

L

G

G

P

P

Q

Q

V

I

K

A

Y

Y

M

M

L

L

K

P

N

E

L

I

S

-

E

Q

K

R

A

L

P

P

L

-

N

N

I

K

P

P

V

V

Q

E

P

V

I

I

N

D

T

S

M

L

D

L

Y

Y

G

G

M

K

Q

V

R

D

G

G

D

L

N

G

V

V

L

L

binding phosphite ion: C8 (= C8), S9 (= S9), A10 (= A10), G11 (= G11), M12 (= M12), S13 (= S13)

P69795 PTS system N,N'-diacetylchitobiose-specific EIIB component; EIIB-Chb; IVcel; N,N'-diacetylchitobiose-specific phosphotransferase enzyme IIB component; EC 2.7.1.196 from Escherichia coli (strain K12) (see 2 papers)
44% identity, 87% coverage: 4:93/103 of query aligns to 6:93/106 of P69795

query
sites
P69795

I

I

M

Y

L

L

C

F

C
|
C

S

S

A

A

G

G

M

M

S

S

T

T

S

S

L

L

V

V

R

S

K

K

M

M

V

R

D

A

A

Q

A

A

K

E

E

K

R

Y

N

E

L

V

P

P

V

V

Q

I

I

I

D

E

A

A

Y

F

G

P

V

E

S

T

E

L

F

A

D

G

M

E

Q

K

F

G

P

Q

Q

N

Y

A

Q

D

V

V

L

L

G

G

P

P

Q

Q

V

I

K

A

Y

Y

M

M

L

L

K

P

N

E

L

I

S

-

E

Q

K

R

A

L

P

P

L

-

N

N

I

K

P

P

V

V

Q

E

P

V

I

I

N

D

T

S

M

L

D

L

Y

Y

G

G

M

K

Q

V

R

D

G

G

D

L

N

G

V

V

L

L

C10 (= C8) active site, Phosphocysteine intermediate; mutation to S: Unable to be phosphorylated by EIIA.

Query Sequence

>BWI76_RS27745 FitnessBrowser__Koxy:BWI76_RS27745
MYKIMLCCSAGMSTSLLVRKMVDAAKERNLPVQIDAYGVSEFDMQFPQYQVVLLGPQVKY
MLKNLSEKAAPLNIPVQPINTMDYGMQRGDNVLDYALSLIAAH

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory