SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing BWI76_RS27755 FitnessBrowser__Koxy:BWI76_RS27755 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 3 hits to proteins with known functional sites (download)

P69791 PTS system N,N'-diacetylchitobiose-specific EIIA component; EIIA-Chb; EIII-Chb; IIIcel; N,N'-diacetylchitobiose-specific phosphotransferase enzyme IIA component from Escherichia coli (strain K12) (see paper)
47% identity, 98% coverage: 3:100/100 of query aligns to 16:113/116 of P69791

query
sites
P69791

E

E

L

L

E

E

D

E

A

V

V

V

M

M

E

G

I

L

I

I

V

I

N

N

A

S

G

G

Q

Q

S

A

R

R

S

S

L

L

C

A

F

Y

E

A

A

A

L

L

H

K

C

Q

A

A

R

K

N

Q

G

G

N

D

I

F

D

A

E

A

A

A

R

K

L

A

L

M

N

D

E

Q

A

S

D

R

G

M

Y

A

A

L

R

N

R

E

A

A

H

H

Q

L

M

V

Q

Q

T

T

R

K

L

L

I

I

E

E

Q

G

D

D

A

A

G

G

E

E

A

G

R

K

Q

M

P

K

M

V

T

S

L

L

I

V

M

L

V

V

H

H

A

A

Q

Q

D
|
D

H

H

L

L

M

M

N

T

S

S

L

M

L

L

A

A

R

R

E

E

F

L

S

I

E

T

E

E

L

L

I

I

H

E

L

L

Y

H

Q

E

R

K

D92 (= D79) mutation to L: Eliminates the need for a metal cation at the homotrimer interface by substituting hydrophobic methyl-methyl interactions in place of the metal cation.

P23532 PTS system lactose-specific EIIA component; EIIA-Lac; EIII-Lac; Lactose-specific phosphotransferase enzyme IIA component from Lactococcus lactis subsp. lactis (Streptococcus lactis) (see 2 papers)
38% identity, 92% coverage: 9:100/100 of query aligns to 11:102/105 of P23532

query
sites
P23532

M

F

E

E

I

I

I

V

V

A

N

Y

A

A

G
|
G

Q

D

S

A

R

R

S

S

L

K

C

L

F

L

E

E

A

A

L

L

H

K

C

A

A

A

R

E

N

N

G

G

N

D

I

F

D

A

E

K

A

A

R

D

L

S

L

L

L

V

N

V

E

E

A

A

D

G

G

S

Y

C

A

I

R

A

R

E

A

A

H

H

Q

S

M

S

Q

Q

T

T

R

G

L

M

I

L

E

A

Q

R

D

E

A

A

G

S

E

G

A

E

R

E

Q

L

P

P

M

Y

T

S

L

V

I

T

M

M

V

M

H

H

A

G

Q

Q

D
|
D

H

H

L

L

M

M

N

T

S

T

L

I

L

L

A

L

R

K

E

D

F

V

S

I

E

H

L

L

I

I

H

E

L

L

Y

Y

Q

K

R

R

G18 (= G16) mutation to E: No activity.
D81 (= D79) binding

P0A0D6 PTS system lactose-specific EIIA component; EIIA-Lac; EIII-Lac; Lactose-specific phosphotransferase enzyme IIA component from Staphylococcus aureus (see 2 papers)
34% identity, 92% coverage: 9:100/100 of query aligns to 11:102/103 of P0A0D6

query
sites
P0A0D6

M

F

E

E

I

I

I

V

V

A

N

F

A

A

G

G

Q

D

S

A

R

R

S

S

L

K

C

F

F

L

E

E

A

A

L

L

H

T

C

A

A

A

R

Q

N

A

G

G

N

D

I

F

D

A

E

K

A

A

R

D

L

A

L

L

L

I

N

E

E

E

A

G

D

N

G

N

Y

C

A

I

R

A

R

E

A

A

H

H

Q

R

M

A

Q

Q

T

T

R

S

L

L

I

L

E

A

Q

K

D

E

A

A

G

Q

E

G

A

D

R

D

Q

I

P

A

M

Y

T

S

L

V

I

T

M

M

V

M

H
|
H

A

G

Q

Q

D

D

H
|
H

L

L

M

M

N

T

S

T

L

I

L

L

A

L

R

K

E

D

F

L

S

M

E

K

E

H

L

L

I

L

H

E

L

F

Y

Y

Q

K

R

R

H78 (= H76) active site, Tele-phosphohistidine intermediate; mutation to S: Abolished activity.
H82 (= H80) mutation to S: Reduced activity.

Query Sequence

>BWI76_RS27755 FitnessBrowser__Koxy:BWI76_RS27755
MIELEDAVMEIIVNAGQSRSLCFEALHCARNGNIDEARLLLNEADGYARRAHQMQTRLIE
QDAGEARQPMTLIMVHAQDHLMNSLLAREFSEELIHLYQR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory