SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing BWI76_RS27880 FitnessBrowser__Koxy:BWI76_RS27880 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 15 hits to proteins with known functional sites (download)

6dvvA 2.25 angstrom resolution crystal structure of 6-phospho-alpha- glucosidase from klebsiella pneumoniae in complex with NAD and mn2+. (see paper)
97% identity, 100% coverage: 1:440/440 of query aligns to 1:435/435 of 6dvvA

query
sites
6dvvA

M

M

K

K

F

F

S

S

V

V

I

I

A

A

G
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G

G

G

G
|
G

S
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S

T
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T

F
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F

T

T

P

P

G

G

I
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I

V

V

L

L

M

M

L

L

A

A

N

N

Q

Q

D

D

R

R

F

F

P

P

L

L

R

R

A

S

L

L

K

K

F

F

Y
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Y

D
|
D

N
|
N

D

D

G

G

A

A

R
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R

Q

Q

E

E

T

T

I

I

A

A

E

E

A

A

C

C

K

K

V

V

I

I

L

L

K

K

E

E

Q

Q

A

A

P

P

E

E

I

I

A

E

F

F

S

S

Y

Y

T

T

D

D

P

P

Q

Q

A

A

F

F

T

T

D

D

V

V

D

D

F

F

V

V

M

M

A

A

H
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H

I
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I

R
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R

V
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V

G

G

K
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K

Y
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Y

P

P

M

M

R

R

E

E

K

Q

D

D

E

E

K

K

I

I

P

P

L

L

R

R

H

H

G

G

V

V

L

L

G

G

Q

Q

E
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E

T

T

C

C

G

G

P

P

G

G

I

I

A

A

Y
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Y

G

G

M

M

R

R

S

S

I

I

G

G

V

V

L

L

E

E

L

L

V

V

D

D

Y

Y

M

M

E

E

K

K

Y

Y

S

S

P

P

N

N

A

A

W

W

M

M

L

L

N

N

Y
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Y

S
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S

N
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N

P

P

A

A

I

I

V

V

A

A

E

E

A

A

T

T

R

R

L

L

R

R

P

P

N

N

A

A

K

K

I

I

L

L

N

N

I

I

C
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C

D

D

M

M

P

P

I

I

G

G

I

I

E

E

G

G

R

R

M

M

A

A

Q

Q

I

I

V

V

G

G

L

L

K

K

D

D

R

R

K

K

Q

Q

M

M

R

R

V

V

R

R

Y

Y

G

G

L

L

N
|
N

H
|
H

F

F

G

G

W

W

T

T

S

S

I

I

E

E

D

D

L

L

Q

D

G

G

N

N

D

D

L

L

M

M

P

P

K

K

L

L

R

R

E

E

Y

Y

V

V

A

A

K

K

Y

Y

G

G

Y

Y

V

V

P

P

S

S

N

-

D

-

P

-

H

-

T

-

E

E

A

A

S

S

W

W

N

N

D

D

T

T

F

F

A

A

K

K

A

A

K

K

D

D

V

V

Q

Q

A

A

L

L

D

D

P

P

D

Q

T

T

M

M

P

P

N

N

T

T

Y

Y

L

L

K

K

Y

Y

L

L

F

F

P

P

D

D

Y

Y

V

V

A

A

H

H

S

S

N

N

P

P

E

E

R

R

T

T

R

R

A

A

N

N

E

E

V

V

M

M

D

D

H

H

R

R

E

E

K

K

N

N

V

V

F

F

S

S

A

A

C

C

R

R

A

A

I

I

A

A

G

G

K

K

S

S

S

T

A

A

G

G

D

D

L

L

E

E

I

I

D

D

E

E

H

H

A

A

S

S

Y

Y

I

I

V

V

D

D

L

L

A

A

A

T

A

A

I

I

A

A

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F

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N

T

T

Q

Q

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E

R

R

M

M

L

L

I

I

V

V

P

P

N

N

G

G

A

A

I

I

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H

N

N

F

F

D

D

A

A

D

D

A

A

M

M

V

V

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E

I

I

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P

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C

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L

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V

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G

H

H

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G

G

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D

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I

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H

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F

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Q

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K

G

G

M

L

M

M

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S

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A

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E

K

K

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D

D

A

A

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W

E

E

Q

Q

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R

S

S

Y

Y

H

H

K

K

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L

W

W

Q

Q

A

A

I

I

A

T

L

L

S

S

K

K

T

T

V

V

P

P

S

S

A

A

S

S

V

V

A

A

K

K

A

A

I

I

L

L

D

D

L

L

I

I

A

A

N

N

K

K

A

D

Y

Y

W

W

P

P

E

E

L

L

H

H

binding manganese (ii) ion: N147 (= N147), C169 (= C169), N199 (= N199), H200 (= H200)
binding nicotinamide-adenine-dinucleotide: G10 (= G10), G12 (= G12), S13 (= S13), T14 (= T14), F15 (= F15), I19 (= I19), Y38 (= Y38), D39 (= D39), N40 (= N40), R44 (= R44), H84 (= H84), I85 (= I85), R86 (= R86), V87 (= V87), K89 (= K89), Y90 (= Y90), E109 (= E109), Y118 (= Y118), Y145 (= Y145), S146 (= S146), N147 (= N147)

Q97LM4 Maltose-6'-phosphate glucosidase MalH; 6-phospho-alpha-glucosidase; 6-phospho-glucosidase; Maltose-6-phosphate hydrolase; EC 3.2.1.122 from Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787) (see paper)
76% identity, 100% coverage: 1:440/440 of query aligns to 1:441/441 of Q97LM4

query
sites
Q97LM4

M

M

K

K

F

F

S

S

V

V

I

I

A

A

G

G

S

S

T

T

F

F

T

T

P

P

G

G

I

I

V

V

L

L

M

M

L

L

A

D

N

N

Q

M

D

D

R

K

F

F

P

P

L

I

R

R

A

K

L

L

K

K

F

F

Y

Y

D

D

N

N

D

D

G

K

A

E

R

R

Q

Q

E

A

T

I

I

V

A

A

E

G

A

A

C

C

K

E

V

I

I

I

L

L

K

K

E

E

Q

K

A

A

P

P

E

E

I

I

A

E

F

F

S

L

Y

A

T

T

D

N

P

P

Q

K

A

E

A

A

F

F

T

T

D

D

V

V

D

D

F

F

V

V

M

M

A

A

H

H

I

I

R

R

V

V

G

G

K

K

Y

Y

P

A

M

M

R

R

E

E

K

L

D

D

E

E

K

K

I

I

P

P

L

L

R

K

H

Y

G

G

V

V

L

V

G

G

Q

Q

E

E

T

T

C

C

G

G

P

P

G

G

I

I

A

A

Y

Y

G

G

M

M

R

R

S

S

I

I

G

G

V

V

L

I

E

E

L

I

V

L

D

D

Y

Y

M

M

E

E

K

K

Y

Y

S

S

P

P

N

N

A

A

W

W

M

M

L

L

N

N

Y

Y

S

S

N

N

P

P

A

A

I

I

V

V

A

A

E

E

A

A

T

T

R

R

R

K

L

L

R

R

P

P

N

N

A

S

K

K

I

I

L

L

N

N

I

I

C
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C

D
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D

M
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M

P
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P

I

I

G

G

I

I

E

E

G

T

R

R

M

M

A

A

Q

E

I

I

V

L

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G

L

L

K

E

D

S

R

R

K

K

Q

E

M

M

R

T

V

V

R

K

Y

Y

G

G

L

L

N

N

H

H

F

F

G

G

W

W

T

S

S

D

I

I

E

R

D

D

L

K

Q

D

G

G

N

N

D

D

L

L

M

M

P

P

K

K

L

L

R

K

E

E

Y

H

V

V

A

K

K

K

Y

Y

G

G

Y

Y

V

V

P

A

P

E

S

N

N

G

D

D

P

T

-

Q

H

H

T

T

E

D

A

A

S

S

W

W

N

N

D

D

T

T

F

F

A

A

K

K

A

A

K

K

D

D

V

V

Q

Y

A

A

L

V

D

D

P

P

D

S

T

T

M

L

P

P

N

N

T

T

Y

Y

L

L

K

K

Y

Y

L

L

F

F

P

P

D

D

Y

Y

V

V

A

E

H

H

S

S

N

N

P

K

E

E

R

Y

T

T

R

R

A

A

N

N

E

E

V

V

M

M

D

D

H

G

R

R

E

E

K

K

N

F

V

V

F

F

S

G

A

E

C

C

R

K

A

K

I

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I

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E

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A

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G

C

D

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I

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D

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E

E

H

H

A

A

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S

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Y

I

I

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V

D

D

L

L

A

A

A

R

A

A

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I

A

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F

Y

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T

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Q

H

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M

M

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L

I

I

V

V

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P

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N

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G

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E

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F

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D

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G

M

M

V

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E

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I

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P

P

C

C

L

I

V

V

G

G

H

S

N

N

G

G

P

P

E

E

P

P

L

L

T

T

V

M

G

G

D

K

I

I

P

P

H

Q

F

F

Q

Q

K

K

G

G

M

L

M

M

S

E

Q

Q

V

V

A

S

V

V

E

E

K

K

L

L

V

V

D

E

A

A

W

W

E

K

Q

E

R

K

S

S

Y

Y

H

Q

K

K

L

L

W

W

Q

Q

A

A

I

I

A

T

L

L

S

S

K

R

T

T

V

V

P

P

S

S

A

A

S

K

V

V

A

A

K

K

A

Q

I

I

L

L

D

D

D

E

L

L

I

I

A

E

A

V

N

N

K

K

A

D

Y

Y

W

W

P

P

E

E

L

L

H

N

C169 (= C169) mutation to S: Loss of activity.
D170 (= D170) mutation to N: Loss of activity.
M171 (= M171) mutation to V: Highly reduced activity.
P172 (= P172) mutation to A: Reduced activity.

P54716 Maltose-6'-phosphate glucosidase; 6-phospho-alpha-D-glucosidase; 6-phosphoryl-O-alpha-D-glucopyranosyl:phosphoglucohydrolase; EC 3.2.1.122 from Bacillus subtilis (strain 168) (see paper)
72% identity, 100% coverage: 2:439/440 of query aligns to 4:441/449 of P54716

query
sites
P54716

K

K

K

S

F

F

S

S

V

I

V

V

I

I

A

A

G

G

S

S

T

T

F

F

T

T

P

P

G

G

I

I

V

V

L

L

M

M

L

L

A

D

N

H

Q

L

D

E

R

E

F

F

P

P

L

I

R

R

A

K

L

L

K

K

F

L

Y

Y

D
|
D

N

N

D

D

G

K

A

E

R

R

Q

Q

E

D

T

R

I

I

A

A

E

G

A

A

C

C

K

D

V

V

I

F

L

I

K

R

E

E

Q

K

A

A

P

P

E

D

I

I

A

E

F

F

S

A

Y

A

T

T

D

D

P

P

Q

E

A

E

A

A

F

F

T

T

D

D

V

V

D

D

F

F

V

V

M

M

A

A

H

H

I

I

R

R

V

V

G

G

K

K

Y

Y

P

A

M

M

R

R

E

A

K

L

D

D

E

E

K

Q

I

I

P

P

L

L

R

K

H

Y

G

G

V

V

L

V

G

G

Q

Q

E
|
E

T

T

C

C

G

G

P

P

G

G

I

I

A

A

Y

Y

G

G

M

M

R

R

S

S

I

I

G

G

V

V

L

L

E

E

L

I

V

L

D

D

Y

Y

M

M

E

E

K

K

Y

Y

S

S

P

P

N

D

A

A

W

W

M

M

L

L

N

N

Y

Y

S

S

N

N

P

P

A

A

I

I

V

V

A

A

E

E

A

A

T

T

R

R

L

L

R

R

P

P

N

N

A

S

K

K

I

I

L

L

N

N

I

I

C

C

D

D

M

M

P

P

I

V

G

G

I

I

E

E

G

D

R

R

M

M

A

A

Q

Q

I

I

V

L

G

G

L

L

K

S

D

S

R

R

K

K

Q

E

M

M

R

K

V

V

R

R

Y

Y

G

G

L

L

N

N

H

H

F

F

G

G

W

W

T

T

S

S

I

I

E

Q

D

D

L

Q

Q

E

G

G

N

N

D

D

L

L

M

M

P

P

K

K

L

L

R

K

E

E

Y

H

V

V

A

S

K

Q

Y

Y

G

G

Y

Y

V

I

P

P

P

K

S

T

N

E

D

A

P

E

H

A

T

V

E

E

A

A

S

S

W

W

N

N

D

D

T

T

F

F

A

A

K

K

A

A

K

R

D

D

V

V

Q

Q

A

A

L

A

D

D

P

P

D

D

T

T

M

L

P

P

N

N

T

T

Y

Y

L

L

K

Q

Y

Y

L

L

F

F

P

P

D

D

Y

D

V

M

V

V

A

K

H

K

S

S

N

N

P

P

E

N

R

H

T

T

R

R

A

A

N

N

E

E

V

V

M

M

D

E

H

G

R

R

E

E

K

A

N

F

V

I

F

F

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S

A

Q

C

C

R

D

A

M

I

I

I

T

A

R

A

E

G

Q

K

S

S

S

S

E

A

N

G

S

D

E

L

I

E

K

I

I

D

D

E

D

H

H

A

A

S

S

Y

Y

I

I

V

V

D

D

L

L

A

A

A

R

A

A

I

I

A

A

F

Y

N

N

T

T

Q

G

E

E

R

R

M

M

L

L

I

I

V

V

P

E

N

N

G

G

A

A

I

I

H

A

N

N

F

F

D

D

A

P

D

T

A

A

M

M

V

V

E
|
E

I

V

P

P

C

C

L

I

V

V

G

G

H

S

N

N

G

G

P

P

E

E

P

P

L

I

T

T

V

V

G

G

D

T

I

I

P

P

H

Q

F

F

Q

Q

K

K

G

G

M

L

M

M

S

E

Q

Q

V

V

A

S

V

V

E

E

K

K

L

L

V

T

V

V

D

E

A

A

W

W

E

A

Q

E

R

K

S

S

Y

F

H

Q

K

K

L

L

W

W

Q

Q

A

A

I

L

A

I

L

L

S

S

K

K

T

T

V

V

P

P

S

N

A

A

S

R

V

V

A

A

K

R

A

L

I

I

L

L

D

E

D

D

L

L

I

V

A

E

A

A

N

N

K

K

A

D

Y

F

W

W

P

P

E

E

L

L

D41 (= D39) mutation D->E,G: Loss of activity.
E111 (= E109) mutation E->D,G: Loss of activity.
E359 (= E357) mutation E->D,G: Loss of activity.

1u8xX Crystal structure of glva from bacillus subtilis, a metal-requiring, NAD-dependent 6-phospho-alpha-glucosidase (see paper)
71% identity, 100% coverage: 2:439/440 of query aligns to 2:432/436 of 1u8xX

query
sites
1u8xX

K

K

K

S

F

F

S

S

V

I

V

V

I

I

A

A

G
|
G

G

G

G
|
G

S
|
S

T
|
T

F
|
F

T

T

P

P

G

G

I

I

V

V

L

L

M

M

L

L

A

D

N

H

Q

L

D

E

R

E

F

F

P

P

L

I

R

R

A

K

L

L

K

K

F

L

Y

Y

D
|
D

N
|
N

D
|
D

G

K

A

E

R

R

Q

Q

E

D

T

R

I

I

A

A

E

G

A

A

C

C

K

D

V

V

I

F

L

I

K

R

E

E

Q

K

A

A

P

P

E

D

I

I

A

E

F

F

S

A

Y

A

T

T

D

D

P

P

Q

E

A

E

A

A

F

F

T

T

D

D

V

V

D

D

F

F

V

V

M

M

A

A

H
|
H

I
|
I

R
|
R

V
|
V

G

G

K

K

Y
|
Y

P

A

M

M

R
|
R

E

A

K

L

D

D

E

E

K

Q

I

I

P

P

L

L

R

K

H

Y

G

G

V

V

L

V

G

G

Q

Q

E
|
E

T

T

C

C

G

G

P

P

G

G

I

I

A

A

Y
|
Y

G

G

M

M

R

R

S

S

I

I

G

G

V

V

L

L

E

E

L

I

V

L

D

D

Y

Y

M

M

E

E

K

K

Y

Y

S

S

P

P

N

D

A

A

W

W

M

M

L

L

N

N

Y
|
Y

S
|
S

N
|
N

P

P

A

A

I

I

V

V

A

A

E

E

A

A

T

T

R

R

L

L

R

R

P

P

N

N

A

S

K

K

I

I

L

L

N

N

I
|
I

C
|
C

D
|
D

M
|
M

P

P

I

V

G

G

I

I

E

E

G

D

R

R

M

M

A

A

Q

Q

I

I

V

L

G

G

L

L

K

S

D

S

R

R

K

K

Q

E

M

M

R

K

V

V

R

R

Y

Y

G

G

L

L

N

N

H
|
H

F

F

G

G

W

W

T

T

S

S

I

I

E

Q

D

D

L

Q

Q

E

G

G

N

N

D

D

L

L

M

M

P

P

K

K

L

L

R

K

E

E

Y

H

V

V

A

S

K

Q

Y

Y

G

G

Y

Y

V

I

P

P

P

-

S

-

N

-

D

-

P

-

H

-

T

-

E

K

A

T

S

S

W

W

N

N

D

D

T

T

F

F

A

A

K

K

A

A

K

R

D

D

V

V

Q

Q

A

A

L

A

D

D

P

P

D

D

T

T

M

L

P

P

N

N

T

T

Y
|
Y

L

L

K

Q

Y

Y

L

L

F

F

P

P

D

D

Y

D

V

M

V

V

A

K

H

K

S

S

N

N

P

P

E

N

R

H

T

T

R
|
R

A

A

N

N

E

E

V
|
V

M

M

D

E

H

G

R
|
R

E

E

K

A

N

F

V

I

F

F

S

S

A

Q

C

C

R

D

A

M

I

I

I

T

A

R

A

E

G

Q

K

S

S

S

S

E

A

N

G

S

D

E

L

I

E

K

I

I

D

D

E

D

H

H

A

A

S

S

Y

Y

I

I

V

V

D

D

L

L

A

A

A

R

A

A

I

I

A

A

F

Y

N

N

T

T

Q

G

E

E

R

R

M

M

L

L

I

I

V

V

P

E

N

N

G

G

A

A

I

I

H

A

N

N

F

F

D

D

A

P

D

T

A

A

M

M

V

V

E

E

I

V

P

P

C

C

L

I

V

V

G

G

H

S

N

N

G

G

P

P

E

E

P

P

L

I

T

T

V

V

G

G

D

T

I

I

P

P

H

Q

F

F

Q

Q

K

K

G

G

M

L

M

M

S

E

Q

Q

V

V

A

S

V

V

E

E

K

K

L

L

V

T

V

V

D

E

A

A

W

W

E

A

Q

E

R

K

S

S

Y

F

H

Q

K

K

L

L

W

W

Q

Q

A

A

I

L

A

I

L

L

S

S

K

K

T

T

V

V

P

P

S

N

A

A

S

R

V

V

A

A

K

R

A

L

I

I

L

L

D

E

D

D

L

L

I

V

A

E

A

A

N

N

K

K

A

D

Y

F

W

W

P

P

E

E

L

L

binding 6-O-phosphono-alpha-D-glucopyranose: R93 (= R93), E109 (= E109), N147 (= N147), D170 (= D170), M171 (= M171), H200 (= H200), Y256 (= Y263), R276 (= R283), V280 (= V287), R284 (= R291)
binding manganese (ii) ion: N147 (= N147), C169 (= C169), H200 (= H200)
binding nicotinamide-adenine-dinucleotide: G10 (= G10), G12 (= G12), S13 (= S13), T14 (= T14), F15 (= F15), D39 (= D39), N40 (= N40), D41 (= D41), H84 (= H84), I85 (= I85), R86 (= R86), V87 (= V87), Y90 (= Y90), E109 (= E109), Y118 (= Y118), Y145 (= Y145), S146 (= S146), N147 (= N147), I168 (= I168), C169 (= C169), D170 (= D170), R284 (= R291)

6vc6B 2.1 angstrom resolution crystal structure of 6-phospho-alpha- glucosidase from gut microorganisms in complex with NAD and mn2+
45% identity, 100% coverage: 2:439/440 of query aligns to 1:439/440 of 6vc6B

query
sites
6vc6B

K

K

F

Y

S

N

V

V

V

C

I

I

A

V

G
|
G

G

G

G
|
G

S
|
S

T
|
T

F
x
Y

T

T

P

P

G

G

I

F

V

L

L

K

M

S

L

F

L

V

A

R

N

L

Q

Q

D

N

R

E

F

F

P

P

L

M

R

E

A

K

L

L

K

V

F

L

Y

F

D
|
D

N
x
I

D
|
D

G

A

A

E

R
|
R

Q

Q

E

Q

T

P

I

I

A

G

E

E

A

F

C

G

K

K

V

I

I

L

L

F

K

S

E

E

Q

R

A

F

P

P

E

E

I

L

A

D

F

F

S

S

Y

Y

T

T

D

D

P

P

Q

A

A

E

A

A

F

Y

T

K

D

D

V

M

D

D

F

F

V

I

M

F

A

M

H
x
Q

I
x
M

R
|
R

V

A

G

G

K

G

Y
x
L

P

P

M

M

R
|
R

E

R

K

E

D

D

E

E

K

H

I

I

P

S

L

L

R

H

H

L

G

G

V

R

L

I

G

G

Q

Q

E
|
E

T

T

C

C

G

G

P

A

G

G

I

M

A

A

Y
|
Y

G

G

M

L

R

R

S

S

I

C

G

V

G
x
D

V

M

L

I

E

E

L

S

V

I

D

H

Y

Q

M

I

E

R

K

Q

Y

Y

S

S

P

P

N

N

A

A

W

W

M

I

L

L

N

N

Y
|
Y

S

S

N
|
N

P

P

A

A

I

I

V

V

A

A

E

E

A

A

T

L

R

R

L

E

R

F

P

P

-

D

N

D

A

N

K

R

I

I

L

L

N

N

I
|
I

C
|
C

D
|
D

M
x
Q

P

P

I

E

G

N

I

I

E

M

G

R

R

S

M

V

A

S

Q

R

I

L

V

L

G

N

L

V

K

S

D

-

R

W

K

E

Q

D

M

L

R

D

V

P

R

V

Y

Y

Y

F

G

G

L

L

N

N

H
|
H

F

Y

G

G

W

W

W

F

T

T

S

H

I

V

E

Y

D

D

L

R

Q

K

-

T

G

G

N

E

D

D

L

L

M

L

P

P

K

E

L

I

R

K

E

K

Y

I

V

I

A

K

K

E

Y

K

G

G

Y

F

V

L

P

P

P

Q

S

D

N

A

D

E

P

-

H

Q

T

R

E

D

A

Q

S

S

W
|
W

N

L

D

D

T

T

F

Y

A

G

K

F

A

V

K

Q

D

T

V

M

Q

M

A

E

L

D

D

F

P

P

D

D

T

F

M

L

P

P

N

N

T

T

Y
|
Y

L

D

K

G

Y

Y

L

L

F

Y

P

P

D

D

Y

Y

V

K

V

F

A

S

H

H

S

L

N

N

P

P

E

D

R

Y

T

T

R
|
R

A

A

N

D

E

E

V
|
V

M

I

D

D

H

G

R
|
R

E

E

K

K

N

R

V

V

F

F

S

A

A

E

C

C

R

R

A

E

I

V

I

I

A

A

A

R

G

G

K

E

-

L

S

G

S

D

A

R

G

F

D

D

L

T

E

I

I

S

D

D

E

A

H
|
H

A

A

S

E

Y

M

I

M

V

I

D

K

L

V

A

A

A

E

A

A

I

I

A

A

F

Y

N

N

T

K

Q

N

E

T

R

R

M

F

L

I

L

V

I

I

V

V

P

K

N

N

N

E

G

G

A

A

I

I

H

A

N

N

F

M

D

Q

A

D

D

D

A

A

M

M

V

V

E

E

I

L

P

V

C

C

L

E

V

L

G

G

H

I

N

N

G

G

P

P

E

R

P

R

L

M

T

A

V

V

G

G

D

N

I

I

P

P

H

Q

F

F

Q

Y

K

L

G

G

M

L

S

V

Q

Q

V

V

A

S

V

S

E

E

K

K

L

L

V

L

V

V

D

D

A

A

W

Y

E

Y

Q

E

R

H

S

S

Y

Y

H

Q

K

K

L

A

W

L

Q

E

A

A

I

F

A

T

L

L

S

N

K

R

T

L

V

I

P

N

S

D

A

A

S

K

V

K

A

A

K

R

A

E

I

I

L

L

D

D

D

A

L

M

I

I

A

E

A

V

N

N

K

K

A

G

Y

M

W

W

P

P

E

E

L

L

binding 6-O-phosphono-alpha-D-glucopyranose: Y14 (≠ F15), R92 (= R93), E108 (= E109), N146 (= N147), D170 (= D170), Q171 (≠ M171), H199 (= H200), W240 (= W241), Y262 (= Y263), R282 (= R283), V286 (= V287), R290 (= R291)
binding manganese (ii) ion: C169 (= C169), H199 (= H200)
binding nicotinamide-adenine-dinucleotide: G9 (= G10), G11 (= G12), S12 (= S13), T13 (= T14), Y14 (≠ F15), D38 (= D39), I39 (≠ N40), D40 (= D41), R43 (= R44), Q83 (≠ H84), M84 (≠ I85), R85 (= R86), L89 (≠ Y90), E108 (= E109), Y117 (= Y118), D124 (≠ G125), Y144 (= Y145), N146 (= N147), I168 (= I168), C169 (= C169), D170 (= D170), R290 (= R291), H318 (= H318)

6wbtA 2.52 angstrom resolution crystal structure of 6-phospho-alpha- glucosidase from gut microorganisms in complex with NAD and glucose- 6-phosphate
46% identity, 100% coverage: 2:439/440 of query aligns to 2:441/442 of 6wbtA

query
sites
6wbtA

K

K

K

E

F

L

S

K

V

I

V

V

I

I

A

C

G
|
G

G

G

G
|
G

S
|
S

T
|
T

F
x
Y

T

T

P

P

G

G

I

I

V

V

L

K

M

D

L

L

A

D

N

Q

Q

R

D

Q

R

K

F

I

P

N

L

I

R

K

A

E

L

L

K

W

F

L

Y

Y

D
|
D

N
x
I

D
|
D

G

E

A

E

R
|
R

Q

Q

E

N

T

K

I

V

A

A

E

L

A

I

C

V

K

K

V

E

I

V

L

I

K

K

E

T

Q

E

A

A

P

P

E

E

I

V

A

V

F

L

S

K

Y

V

T

T

T

V

D

N

P

P

Q

K

A

E

A

A

F

F

T

T

D

D

V

A

D

D

F

Y

V

I

M

M

A

A

H
x
Q

I
x
M

R
|
R

V
|
V

G

G

K

G

Y
x
L

P

K

M

M

R
|
R

E

V

K

K

D

D

E

E

K

Q

I

I

P

C

L

L

R

K

H

H

G

G

V

C

L

V

G

G

Q

Q

E
|
E

T

T

C

C

G

G

P

A

G

G

I

M

A

T

Y
|
Y

G

G

M

M

R

R

S

T

I

I

G

Y

G

P

V

M

L

V

E

Q

L

L

V

I

D

D

Y

Y

M

C

E

E

K

E

Y

Y

-

A

S

S

P

K

N

K

A

Y

W

W

M

I

L

V

N

N

Y
|
Y

S
|
S

N
|
N

P

P

A

A

I

I

V

V

A

A

E

K

A

A

T

T

R

Y

R

K

L

L

R

R

P

P

N

K

A

A

K

R

I

I

L

I

N

N

I

I

C
|
C

D
|
D

M
|
M

P

P

I

V

G

E

I

I

E

E

G

A

R

R

M

M

A

A

Q

E

I

I

V

L

G

D

L

C

K

K

D

-

R

L

K

E

Q

D

M

I

R

E

V

S

R

D

Y

Y

Y

F

G

G

L

L

N

N

H
|
H

F

Y

G

G

W

W

W

F

T

T

S

H

I

V

E

R

D

-

L

C

Q

K

G

G

N

V

D

D

L

V

M

T

P

D

K

K

L

L

R

K

E

E

Y

H

V

V

A

R

K

K

Y

Y

G

G

Y

Y

V

V

P

S

P

E

S

A

-

S

-

M

N

N

D

D

P

A

H

L

-

L

T

K

E

D

A

P

S

D

W
|
W

N

V

D

H

T

T

F

F

A

K

K

N

A

S

K

A

D

L

V

I

Q

S

A

S

L

M

D

F

P

T

D

D

T

Y

M

L

P

P

N

N

T

T

Y
|
Y

L

W

K

Q

Y

Y

L

L

F

M

P

P

D

D

Y

S

V

I

V

V

A

D

H

Y

S

M

N

D

P

I

E

N

R

N

T

T

R
|
R

A

G

N

M

E

Q

V
|
V

M

I

D

N

H

G

R
|
R

E

E

K

K

N

R

V

I

F

F

S

K

A

A

C

A

R

E

A

D

I

I

I

R

A

E

A

G

G

K

K

P

S

V

S

D

A

L

G

Q

D

Q

L

F

E

Y

I

V

D

G

E

V

H
|
H

A

G

S

K

Y

F

I

I

V

V

D

K

L

V

A

V

A

E

A

S

I

L

A

I

F

H

N

D

T

E

Q

R

E

S

R

R

M

Q

L

L

L

V

I

I

V

V

P

P

N

N

G

G

A

A

I

I

H

E

N

N

F

L

D

S

A

D

D

D

A

A

M

T

V

V

E

E

I

I

P

P

C

G

L

Y

V

V

G

T

H

D

N

R

G

G

P

V

E

E

P

P

L

V

T

R

V

V

G

G

D

S

I

I

P

P

H

R

F

F

Q

Y

K

K

G

G

M

L

M

I

S

E

Q

Q

V

D

A

A

V

C

E

E

K

G

L

L

V

L

V

V

D

E

A

A

W

A

E

I

Q

E

R

H

S

S

Y

Y

H

E

K

K

L

A

W

L

Q

M

A

A

I

F

A

T

L

M

S

N

K

R

T

T

V

I

P

P

S

S

A

S

S

L

V

V

A

A

K

K

A

K

I

L

L

L

D

D

L

M

I

I

A

E

A

A

N

N

K

K

A

G

Y

Y

W

W

P

P

E

E

L

L

binding 6-O-phosphono-alpha-D-glucopyranose: Y15 (≠ F15), R93 (= R93), E109 (= E109), N148 (= N147), D171 (= D170), M172 (= M171), H200 (= H200), W243 (= W241), Y265 (= Y263), R285 (= R283), V289 (= V287), R293 (= R291)
binding manganese (ii) ion: N148 (= N147), C170 (= C169), H200 (= H200)
binding nicotinamide-adenine-dinucleotide: G10 (= G10), G12 (= G12), S13 (= S13), T14 (= T14), Y15 (≠ F15), D39 (= D39), I40 (≠ N40), D41 (= D41), R44 (= R44), Q84 (≠ H84), M85 (≠ I85), R86 (= R86), V87 (= V87), L90 (≠ Y90), E109 (= E109), Y118 (= Y118), Y146 (= Y145), S147 (= S146), N148 (= N147), C170 (= C169), D171 (= D170), R293 (= R291), H320 (= H318)

5c3mB Crystal structure of gan4c, a gh4 6-phospho-glucosidase from geobacillus stearothermophilus
29% identity, 98% coverage: 10:439/440 of query aligns to 7:410/411 of 5c3mB

query
sites
5c3mB

G

G

S

S

T

S

F

Y

T

T

P

P

G

E

I

L

V

V

L

E

M

G

L

L

L

I

A

K

N

R

Q

Y

D

H

R

E

F

L

P

P

L

V

R

G

A

E

L

L

K

W

F

L

Y

V

D

D

N

I

D

P

G

E

A

G

R

K

Q

E

-

K

-

L

E

E

T

I

I

V

A

G

E

A

A

L

C

A

K

K

V

R

I

M

L

V

K

E

E

K

Q

A

A

G

P

V

E

P

I

I

A

E

F

I

S

H

Y

L

T

T

T

L

D

D

P

R

Q

R

A

R

A

A

F

L

T

E

D

G

V

A

D

D

F

F

V

V

M

T

A

T

H

Q

I

F

R

R

V

V

G

G

K

G

Y

L

P

E

M

A

R

R

E

A

K

K

D

D

E

E

K

R

I

I

P

P

L

L

R

K

H

Y

G

G

V

V

L

I

G

G

Q

Q

E

E

T

T

C

N

G

G

P

P

G

G

I

L

A

F

Y

K

G

G

M

L

R

R

S

T

I

I

G

P

G

V

V

I

L

L

E

D

L

I

V

I

D

R

Y

D

M

M

E

E

K

E

Y

L

S

C

P

P

N

D

A

A

W

W

M

L

L

I

N

N

Y

F

S

T

N
|
N

P

P

A

A

A

G

I

M

V

V

A

T

E

E

A

A

T

V

R

L

R

R

L

Y

R

T

P

K

N

Q

A

E

K

K

I

V

L

V

N

G

I

L

C
|
C

D

N

M

V

P

P

I

I

G

G

I

M

E

R

G

M

R

G

M

V

A

A

Q

K

I

L

V

L

G

G

L

V

K

-

D

D

R

A

K

D

Q

R

M

V

R

H

V

I

R

D

Y

F

Y

A

G

G

L

L

N

N

H
|
H

F

M

G

V

W

F

W

G

T

L

S

H

I

V

E

Y

D

-

L

L

Q

D

G

G

N

V

D

E

L

V

M

T

P

E

K

K

L

V

R

I

E

D

Y

L

V

V

A

A

K

H

Y

-

G

-

Y

-

V

-

P

-

S

-

N

-

D

-

P

-

H

-

T

-

E

P

A

L

S

G

W

W

N

E

D

P

T

D

F

F

A

L

K

K

A

G

K

L

D

K

V

V

Q

-

A

-

L

-

D

-

P

-

D

-

T

-

M

L

P

P

N

C

T

P

Y

Y

L

H

K

R

Y

Y

L

Y

F

Q

P

T

D

D

Y

K

V

M

V

L

A

A

H

E

-

E

-

L

-

E

-

A

S

A

N

K

P

T

E

K

R

G

T

T

R

R

A

A

N

-

E

E

V

V

M

V

D

Q

H

Q

R

L

E

E

K

K

N

E

V

L

F

F

S

E

A

L

C

Y

R

K

A

-

I

-

A

-

G

-

K

D

S

P

S

R

A

G

G

G

D

A

L

Y

E
x
Y

I

S

D

D

E

A

H

A

A

C

S

S

Y

L

I

I

V

-

D

-

L

-

A

-

A

S

I

I

A

Y

F

N

N

D

T

K

Q

R

E

D

R

I

M

Q

L

P

L

V

I

N

V

T

P

R

N

N

G

G

A

A

I

I

H

A

N

S

F

I

D

P

A

P

D

E

A

S

M

A

V

V

E

E

I

V

P

N

C

C

L

V

V

I

G

T

H

K

N

D

G

G

P

P

E

K

P

P

L

I

T

A

V

V

G

G

D

D

I

L

P

P

H

V

F

A

Q

V

K

R

G

G

M

L

M

V

S

Q

Q

Q

Q

I

V

K

A

S

V

F

E

E

K

R

L

V

V

A

D

E

A

A

W

A

E

V

Q

T

R

G

S

D

Y

Y

H

Q

K

T

L

A

W

L

Q

V

A

A

I

M

A

T

L

I

S

N

K

P

T

L

V

V

P

P

S

S

A

D

S

T

V

I

A

A

K

K

A

Q

I

M

L

L

D

D

D

E

L

M

I

L

A

E

A

A

N

H

K

K

A

E

Y

H

W

L

P

P

E

Q

L

F

binding manganese (ii) ion: N146 (= N147), C168 (= C169), H198 (= H200), Y289 (≠ E314)

1up7A Structure of the 6-phospho-beta glucosidase from thermotoga maritima at 2.4 angstrom resolution in the tetragonal form with NAD and glucose-6-phosphate (see paper)
28% identity, 98% coverage: 6:435/440 of query aligns to 5:410/414 of 1up7A

query
sites
1up7A

V

I

V

A

I

V

A

I

G
|
G

G

G

G
|
G

S
|
S

T

S

F
x
Y

T

T

P

P

G

E

I

L

V

V

L

K

M

G

L

L

A

D

N

I

Q

S

D

E

R

D

F

V

P

R

L

I

R

D

A

E

L

V

K

I

F

F

Y
|
Y

D
|
D

N
x
I

D

D

G

E

A

E

R
x
K

Q

Q

E

K

T

I

I

V

A

V

E

D

A

F

C

V

K

K

V

R

I

L

L

V

K

K

E

D

Q

R

A

F

P

-

E

K

I

V

A

L

F

I

S

S

Y

D

T

T

T

F

D

-

P

-

Q

E

A

G

A

A

F

V

T

V

D

D

V

A

D

K

F

Y

V

V

M

I

A

F

H
x
Q

I
x
F

R
|
R

V

P

G

G

K

G

Y

L

P

K

M

G

R
|
R

E

E

K

N

D

D

E

E

K

G

I

I

P

P

L

L

R

K

H

Y

G

G

V

L

L

I

G

G

Q

Q

E
|
E

T

T

C

T

G

G

P

V

G

G

I

F

A

S

Y

A

G

A

M

L

R

R

S

A

I

F

G

P

G
x
I

V

V

L

E

E

E

L
x
Y

V

V

D

D

Y

T

M

V

E

R

K

K

Y

T

S

S

P

-

N

N

A

A

W

T

M

I

L

V

N

N

Y
x
F

S
x
T

N
|
N

P

P

A

S

A

G

I

H

V

I

A

T

E

E

A

F

T

V

R

R

R

N

L

Y

R

L

P

E

N

Y

A

E

K

K

I

F

L

I

N

G

I

L

C

C

D
x
N

M

V

P

P

I

I

G

N

I

F

E

I

G

R

R

E

M

I

A

A

Q

E

I

M

V

F

G

S

L

A

K

R

D

-

R

L

K

E

Q

D

M

V

R

F

V

L

R

K

Y

Y

G

G

L

L

N

N

H
|
H

F

L

G

S

W

F

W

I

T

E

S

K

I

V

E

-

D

F

L

V

Q

K

G

G

N

E

D

D

L

V

M

T

P

E

K

K

L

V

R

F

E

E

Y

N

V

L

A

K

K

L

Y

-

G

-

Y

K

V

I

P

P

P

D

S

E

N

D

D

F

P

P

H

-

T

-

E

-

A

-

S

T

W

W

N

F

D

Y

T

D

F

S

A

V

K

R

A

-

K

-

D

-

V

-

Q

-

A

-

L

-

D

-

P

-

D

-

T

L

M

I

P

V

N

N

T

P

Y
|
Y

L

L

K

R

Y

Y

L

L

F

M

P

E

D

K

Y

K

V

M

V

F

A

K

H

K

S

I

N

S

P

T

E

H

R

E

T

L

R
|
R

A

A

N

R

E

E

V
|
V

M

M

D

-

H

K

R

I

E
|
E

K

K

N

E

V

L

F

F

S

E

A

K

C

Y

R

R

A

T

I

A

I

V

-

E

-

I

-

P

-

E

-

L

-

T

-

K

-
x
R

-
x
G

-

S

-

M

-
x
Y

-

S

A

T

A

A

G

A

K

A

S

H

S

L

A

I

G

R

D

D

L

L

E

E

I

T

D

D

E

E

H

G

A

K

S

I

Y

H

I

I

V

V

D

-

L

-

A

-

I

-

A

-

F

-

N

N

T

T

Q

R

E

-

R

-

M

-

L

-

I

-

V

-

P

-

N

N

G

G

A

S

I

I

H

E

N

N

F

L

D

P

A

D

D

D

A

Y

M

V

V

L

E

E

I

I

P

P

C

C

L

Y

V

V

G

R

H

S

N

G

G

R

P

V

E

H

P

T

L

L

T

S

V

Q

G

G

D

K

I

G

P

D

H

H

F

F

Q

A

K

L

G

S

M

F

M

I

S

H

Q

A

Q

V

V

K

A

M

V

Y

E

E

K

R

L

L

V

T

V

I

D

E

A

A

W

Y

E

L

Q

K

R

R

S

S

Y

K

H

K

K

L

L

A

W

L

Q

K

A

A

I

L

A

L

L

S

S

H

K

P

T

L

V

G

P

P

S

D

A

V

S

E

V

D

A

A

K

K

A

D

I

L

L

L

D

E

L

I

I

L

A

E

A

A

N

N

K

R

A

E

Y

Y

binding 6-O-phosphono-alpha-D-glucopyranose: Y14 (≠ F15), R89 (= R93), E105 (= E109), N142 (= N147), N165 (≠ D170), H194 (= H200), Y240 (= Y263), R260 (= R283), V264 (= V287), R288 (vs. gap), G289 (vs. gap), Y293 (vs. gap)
binding nicotinamide-adenine-dinucleotide: G9 (= G10), G11 (= G12), S12 (= S13), Y14 (≠ F15), Y37 (= Y38), D38 (= D39), I39 (≠ N40), K43 (≠ R44), Q80 (≠ H84), F81 (≠ I85), R82 (= R86), E105 (= E109), I121 (≠ G125), Y125 (≠ L129), F140 (≠ Y145), T141 (≠ S146), N142 (= N147), E268 (= E292), R288 (vs. gap), G290 (vs. gap), Y293 (vs. gap)

1up6A Structure of the 6-phospho-beta glucosidase from thermotoga maritima at 2.55 angstrom resolution in the tetragonal form with manganese, NAD+ and glucose-6-phosphate (see paper)
28% identity, 98% coverage: 6:435/440 of query aligns to 4:409/413 of 1up6A

query
sites
1up6A

V

I

V

A

I

V

A

I

G
|
G

G

G

G
|
G

S
|
S

T

S

F
x
Y

T

T

P

P

G

E

I

L

V

V

L

K

M

G

L

L

A

D

N

I

Q

S

D

E

R

D

F

V

P

R

L

I

R

D

A

E

L

V

K

I

F

F

Y
|
Y

D
|
D

N
x
I

D
|
D

G
x
E

A

E

R
x
K

Q

Q

E

K

T

I

I

V

A

V

E

D

A

F

C

V

K

K

V

R

I

L

L

V

K

K

E

D

Q

R

A

F

P

-

E

K

I

V

A

L

F

I

S

S

Y

D

T

T

T

F

D

-

P

-

Q

E

A

G

A

A

F

V

T

V

D

D

V

A

D

K

F

Y

V

V

M

I

A

F

H
x
Q

I
x
F

R
|
R

V
x
P

G

G

K

G

Y
x
L

P

K

M

G

R
|
R

E

E

K

N

D

D

E

E

K

G

I

I

P

P

L

L

R

K

H

Y

G

G

V

L

L

I

G

G

Q

Q

E
|
E

T

T

C

T

G

G

P

V

G

G

I

F

A

S

Y

A

G

A

M

L

R

R

S

A

I

F

G

P

G
x
I

V

V

L

E

E

E

L
x
Y

V

V

D

D

Y

T

M

V

E

R

K

K

Y

T

S

S

P

-

N

N

A

A

W

T

M

I

L

V

N

N

Y
x
F

S
x
T

N
|
N

P

P

A

S

A

G

I

H

V

I

A

T

E

E

A

F

T

V

R

R

R

N

L

Y

R

L

P

E

N

Y

A

E

K

K

I

F

L

I

N

G

I
x
L

C
|
C

D
x
N

M

V

P

P

I

I

G

N

I

F

E

I

G

R

R

E

M

I

A

A

Q

E

I

M

V

F

G

S

L

A

K

R

D

-

R

L

K

E

Q

D

M

V

R

F

V

L

R

K

Y

Y

G

G

L

L

N

N

H
|
H

F

L

G

S

W

F

W

I

T

E

S

K

I

V

E

-

D

F

L

V

Q

K

G

G

N

E

D

D

L

V

M

T

P

E

K

K

L

V

R

F

E

E

Y

N

V

L

A

K

K

L

Y

-

G

-

Y

K

V

L

P

P

P

D

S

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N

D

D

F

P

P

H

-

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-

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-

A

-

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W

W

N

F

D

Y

T

D

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K

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A

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K

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-

A

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L

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D

-

P

-

D

-

T

L

M

I

P

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N

N

T

P

Y
|
Y

L

L

K

R

Y

Y

L

L

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M

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M

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L

R
|
R

A

A

N

R

E

E

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|
V

M

M

D

-

H

K

R

I

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|
E

K

K

N

E

V

L

F

F

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E

A

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C

Y

R

R

A

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I

A

I

V

-

E

-

I

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P

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L

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x
G

-

G

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M

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Y

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A

T

A

A

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A

K

A

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H

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A

I

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D

D

L

L

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D

D

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A

K

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Y

H

I

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-

L

-

A

-

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A

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N

N

T

T

Q

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E

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R

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M

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L

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-

N

N

G

G

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N

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L

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A

D

D

D

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L

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C

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N

G

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L

L

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L

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A

A

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Y

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L

Q

K

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R

S

S

Y

K

H

K

K

L

L

A

W

L

Q

K

A

A

I

L

A

L

L

S

S

H

K

P

T

L

V

G

P

P

S

D

A

V

S

E

V

D

A

A

K

K

A

D

I

L

L

L

D

E

L

I

I

L

A

E

A

A

N

N

K

R

A

E

Y

Y

binding 6-O-phosphono-alpha-D-glucopyranose: Y13 (≠ F15), R88 (= R93), E104 (= E109), N141 (= N147), N164 (≠ D170), H193 (= H200), Y239 (= Y263), R259 (= R283), V263 (= V287), G288 (vs. gap)
binding manganese (ii) ion: I38 (≠ N40), I38 (≠ N40), D39 (= D41), D39 (= D41), E40 (≠ G42), C163 (= C169), N164 (≠ D170), H193 (= H200)
binding nicotinamide-adenine-dinucleotide: G8 (= G10), G10 (= G12), S11 (= S13), Y13 (≠ F15), Y36 (= Y38), D37 (= D39), I38 (≠ N40), D39 (= D41), K42 (≠ R44), Q79 (≠ H84), F80 (≠ I85), R81 (= R86), P82 (≠ V87), L85 (≠ Y90), E104 (= E109), I120 (≠ G125), Y124 (≠ L129), F139 (≠ Y145), T140 (≠ S146), N141 (= N147), L162 (≠ I168), C163 (= C169), N164 (≠ D170), E267 (= E292)

P39130 Alpha-galacturonidase; EC 3.2.1.67 from Bacillus subtilis (strain 168) (see paper)
24% identity, 74% coverage: 72:396/440 of query aligns to 74:406/446 of P39130

query
sites
P39130

Q

K

A

K

A

A

F

L

T

S

D

A

V

A

D

D

F

I

V

V

M

I

A

I

H

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I

I

R

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G

G

K

S

Y

L

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D

M

D

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D

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H

I

L

P

P

L

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C

G

G

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I

L

Y

G

Q

Q

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-

V

-

G

E

D

T

T

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V

G

G

P

P

G

G

I

I

A

I

Y

R

G

G

M

L

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R

S

A

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G

P

G

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F

L

A

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E

L

I

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A

D

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A

M

I

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K

D

Y

Y

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A

P

P

N

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A

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W

W

M

V

L

I

N

N

Y

Y

S

T

N

N

P

P

A

M

A

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I

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V

C

A

T

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R

A

V

T

L

R

Y

R

K

L

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F

P

P

N

G

A

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K

I

A

L

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N

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x
C

C

C

D

H

M

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G

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Q

G

K

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L

M

L

A

A

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V

G

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-

L

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M

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G

L

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N

N

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H

F

F

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W

W

W

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-

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L

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I

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A

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D

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L

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D

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E

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N

Y

I

I

I

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A

D

A

L

L

A

L

A

G

A

L

I

G

A

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F

L

N

V

T

T

Q

N

E

V

R

N

M

M

L

-

I

-

V

-

P

P

N

N

N

Q

G

G

A

Q

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V

H

L

N

N

F

L

D

P

A

I

D

Q

A

A

M

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V

V

E

E

I

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P

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C

A

L

F

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G

T

H

R

N

N

G

R

P

V

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P

P

L

I

T

L

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S

G

G

D

A

I

L

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P

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G

M

M

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M

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A

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E

L

A

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V

V

A

D

D

A

A

C172 (≠ I168) mutation to L: Does not significantly affect alpha-galacturonidase activity.

3fefA Crystal structure of putative glucosidase lpld from bacillus subtilis
24% identity, 74% coverage: 72:396/440 of query aligns to 68:400/434 of 3fefA

query
sites
3fefA

Q

K

A

K

A

A

F

L

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D

A

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A

D

D

F

I

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I

A

I

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I

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P

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G

K

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L

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M

D

R

M

E

E

K

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D

D

E

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K

H

I

L

P

P

L

E

R

R

H

C

G

G

V

I

L

Y

G

Q

Q

S

-

V

-

G

E

D

T

T

C

V