SitesBLAST

P16250 Phosphoribosyl isomerase A; 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase; N-(5'-phosphoribosyl)anthranilate isomerase; PRAI; Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase; EC 5.3.1.16; EC 5.3.1.24 from Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) (see paper)
30% identity, 96% coverage: 1:240/249 of query aligns to 4:237/240 of P16250

query
sites
P16250

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D11 (= D8) mutation to A: No activity.
R19 (= R16) mutation to A: No effect on activity toward PRA. No activity toward ProFAR.
S81 (≠ G79) mutation to T: No activity toward PRA. Almost no effect on activity toward ProFAR.
D130 (= D134) mutation to A: Very low activity toward PRA. No activity toward ProFAR.; mutation to Q: No activity.
T166 (= T171) mutation to A: No activity.
D171 (= D176) mutation to A: Low activity toward PRA. No activity toward ProFAR.

4tx9A Crystal structure of hisap from streptomyces sviceus with degraded profar (see paper)
30% identity, 96% coverage: 1:240/249 of query aligns to 9:242/246 of 4tx9A

query
sites
4tx9A

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active site: D16 (= D8), D135 (= D134)
binding aminoimidazole 4-carboxamide ribonucleotide: G28 (= G20), V57 (≠ I50), L59 (= L52), S86 (≠ G79), G88 (= G81), R90 (= R83), G109 (= G102), T110 (= T103), D135 (= D134), G145 (= G144), W146 (= W145)

Q9X0C7 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase; Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase; EC 5.3.1.16 from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) (see paper)
27% identity, 97% coverage: 1:241/249 of query aligns to 1:239/241 of Q9X0C7

query
sites
Q9X0C7

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D8 (= D8) mutation to N: Loss of activity.
H48 (= H48) mutation to A: Decrease in activity.
D51 (= D51) mutation to N: Decrease in activity.
R83 (= R83) mutation to N: Decrease in activity.
D127 (= D134) mutation to N: Almost no activity.
T164 (= T171) mutation to A: Strong decrease in activity.

1h5yB Hisf protein from pyrobaculum aerophilum (see paper)
28% identity, 86% coverage: 1:213/249 of query aligns to 5:215/253 of 1h5yB

query
sites
1h5yB

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active site: D12 (= D8), D133 (= D134)
binding glycerol: K22 (≠ R18), N106 (≠ G102), A131 (= A132), D133 (= D134), G147 (= G144), T174 (= T171), D179 (= D176)
binding phosphate ion: G84 (= G80), G85 (= G81), N106 (≠ G102), T107 (= T103), D179 (= D176), G180 (= G177)

Sites not aligning to the query:

binding phosphate ion: 226, 227, 228

7ac8A Molecular basis for the unique allosteric activation mechanism of the heterodimeric imidazole glycerol phosphate synthase complex. (see paper)
25% identity, 96% coverage: 3:241/249 of query aligns to 6:240/252 of 7ac8A

query
sites
7ac8A

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W

Y

active site: D11 (= D8), D130 (= D134)
binding [(2R,3S,4R,5R)-5-[4-aminocarbonyl-5-[(E)-[[(2R,3R,4S,5R)-3,4-bis(oxidanyl)-5-(phosphonooxymethyl)oxolan-2-yl]amino]methylideneamino]imidazol-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methyl dihydrogen phosphate: L50 (≠ I50), I52 (≠ L52), G82 (= G81), N103 (≠ G102), T104 (= T103), D130 (= D134), S144 (= S143), D176 (= D176), G177 (= G177), S201 (= S202), S225 (≠ K226)

Q9X0C6 Imidazole glycerol phosphate synthase subunit HisF; IGP synthase cyclase subunit; IGP synthase subunit HisF; ImGP synthase subunit HisF; IGPS subunit HisF; EC 4.3.2.10 from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) (see paper)
25% identity, 96% coverage: 3:241/249 of query aligns to 6:240/253 of Q9X0C6

query
sites
Q9X0C6

I

I

P

A

A
x
C

I

L

D
|
D

I

V

L

K

D

D

G

G

K

R

V

V

R
x
K

L

-

R

-

E

-

G

G

D

T

Y

N

N

F

Q

E

K

N

T

L

E

R

Y

D

A

S

V

G

S

D

I

P

P

V

E

E

M

L

I

G

E

K

K

F

Y

Y

R

S

S

E

N

I

G

G

T

I

D

D

F

E

I

L

H

V

I

F

I

L

D
|
D

L

I

N

T

G

A

A

S

K

-

G

-

D

-

F

V

S

E

N

K

Q

R

K

K

T

T

L

M

F

L

E

E

I

L

I

V

K

E

K

K

T

V

E

A

M

E

K

Q

V

I

-

D

-

I

-

P

-

F

Q

T

Y

V

G

G

I

I

R

H

T

D

I

F

E

E

Q

T

V

A

T

S

N

E

L

L

L

I

D

L

A

R

G

G

I

A

H

D

R

K

V

V

I

S

V

I

G
x
N

T

T

Q

A

A

A

I

V

T

E

N

N

P

P

D

S

F

L

L

I

P

T

Q

Q

L

I

S

A

E

Q

A

T

F

F

A

G

K

-

D

-

Y

-

A

S

N

Q

R

A

I

V

V

V

I

V

A

A

I

I

D
|
D

V

A

-

K

-

R

L

V

D

D

E

G

-

E

-

F

-

M

V

V

I

F

K

T

Y

Y

S

S

G

G

W

-

M

K

E

K

S

N

S

T

P

G

I

I

K

L

L

L

M

R

D

D

Y

W

V

V

D

V

K

E

C

V

L

E

S

K

L

R

G

G

F

A

F

G

R

E

F

I

L

L

C

L

T

T

D

S

I

I

S

D

K

R

D
|
D

G

G

K

T

L

K

G

S

G

G

A

Y

A
x
D

I

T

D

E

L

M

Y

I

E

-

K

R

L

F

L

V

E

R

H

P

S

L

P

T

M

T

I

L

K

P

L

I

I

I

A

A

S

S

G

G

V

A

S

G

S

K

M

M

Q

E

D

H

I

F

Y

L

D

E

L

A

A

F

K

L

Y

A

P

G

I

A

R

D

S

A

V

A

V

L

V

A

G

A

K

S

A

V

I

F

Y

H

E

F

D

R

R

E

I

I

T

D

I

V

E

R

E

E

I

L

K

K

E

E

W

Y

C9 (≠ A6) mutation to A: No change in activity.
D11 (= D8) mutation to X: Loss of activity.
K19 (≠ R16) mutation to S: Decrease in activity.
D51 (= D51) mutation to N: No change in activity.
N103 (≠ G102) mutation to A: No change in activity.
D130 (= D134) mutation D->A,C,F,G,H,I,K,L,M,N,P,Q,R,S,T,V,W,Y: Complete loss of activity.; mutation to E: Weak activity.
D176 (= D176) mutation to N: Decrease in activity.
D183 (≠ A183) mutation to N: No change in activity.

3zr4E Structural evidence for ammonia tunneling across the (beta-alpha)8 barrel of the imidazole glycerol phosphate synthase bienzyme complex (see paper)
25% identity, 96% coverage: 3:241/249 of query aligns to 6:231/244 of 3zr4E

query
sites
3zr4E

I

I

P

A

A

C

I

L

D
|
D

I

V

L

K

D

D

G

G

K

R

V

V

R

K

L

-

R

-

E

S

G

G

D

D

Y

-

N

-

Q

-

K

-

T

-

E

-

Y

-

A

-

V

-

S

-

I

-

P

P

E

V

M

E

I

L

E

G

K

K

Y

F

R

Y

S

S

N

E

-

I

G

G

T

I

D

D

F

E

I

L

H

V

I

F

I

L

D

D

L

I

N

T

G

A

A

S

K

-

G

-

D

-

F

V

S

E

N

K

Q

R

K

K

T

T

L

M

F

L

E

E

I

L

I

V

K

E

K

K

T

V

E

A

M

E

K

Q

V

I

-

D

-

I

-

P

-

F

Q

T

Y

V

G

G

I

I

R

H

T

D

I

F

E

E

Q

T

V

A

T

S

N

E

L

L

L

I

D

L

A

R

G

G

I

A

H

D

R

K

V

V

I

S

V

I

G

N

T

T

Q

A

A

A

I

V

T

E

N

N

P

P

D

S

F

L

L

I

P

T

Q

Q

L

I

S

A

E

Q

A

T

F

F

A

G

K

-

D

-

Y

-

A

S

N

Q

R

A

I

V

V

V

I

V

A

A

I

I

D
|
D

V

A

-

K

-

R

L

V

D

D

E

G

-

E

-

F

-

M

V

V

I

F

K

T

Y

Y

S

S

G

G

W

-

M

K

E

K

S

N

S

T

P

G

I

I

K

L

L

L

M

R

D

D

Y

W

V

V

D

V

K

E

C

V

L

E

S

K

L

R

G

G

F

A

F

G

R

E

F

I

L

L

C

L

T

T

D

S

I

I

S

D

K

R

D

D

G

G

K

T

L

K

G

S

G

G

A

Y

A

D

I

T

D

E

L

M

Y

I

E

-

K
x
R

L

F

L

V

E

R

H

P

S

L

P

T

M

T

I

L

K

P

L

I

I

I

A

A

S

S

G

G

V

A

S

G

S

K

M

M

Q

E

D

H

I

F

Y

L

D

E

L

A

A

F

K
x
L

Y

A

P

G

I

A

R

D

S

A

V

A

V

L

V

A

G

A

K

S

A

V

I

F

Y

H

E

F

D

R

R

E

I

I

T

D

I

V

E

R

E

E

I

L

K

K

E

E

W

Y

active site: D11 (= D8), D121 (= D134)
binding glycerol: R179 (≠ K189), L206 (≠ K216)

1gpwC Structural evidence for ammonia tunneling across the (beta/alpha)8 barrel of the imidazole glycerol phosphate synthase bienzyme complex. (see paper)
24% identity, 96% coverage: 3:241/249 of query aligns to 6:240/253 of 1gpwC

query
sites
1gpwC

I

I

P

A

A

C

I

L

D
x
N

I

V

L

K

D

D

G

G

K

R

V

V

R

K

L

-

R

-

E

-

G

G

D

T

Y

N

N

F

Q

E

K

N

T

L

E

R

Y

D

A

S

V

G

S

D

I

P

P

V

E

E

M

L

I

G

E

K

K

F

Y

Y

R

S

S

E

N

I

G

G

T

I

D

D

F

E

I

L

H

V

I

F

I

L

D

D

L

I

N

T

G

A

A

S

K

-

G

-

D

-

F

V

S

E

N

K

Q

R

K

K

T

T

L

M

F

L

E

E

I

L

I

V

K

E

K

K

T

V

E

A

M

E

K

Q

V

I

-

D

-

I

-

P

-

F

Q

T

Y

V

G

G

G
|
G

I

I

R

H

T

D

I

F

E

E

Q

T

V

A

T

S

N

E

L

L

L

I

D

L

A

R

G

G

I

A

H

D

R

K

V

V

I

S

V

I

G
x
N

T
|
T

Q

A

A

A

I

V

T

E

N

N

P

P

D

S

F

L

L

I

P

T

Q

Q

L

I

S

A

E

Q

A

T

F

F

A

G

K

-

D

-

Y

-

A

S

N

Q

R

A

I

V

V

V

I

V

A

A

I

I

D
|
D

V

A

-

K

-

R

L

V

D

D

E

G

-

E

-

F

-

M

V

V

I

F

K

T

Y

Y

S

S

G

G

W

-

M

K

E

K

S

N

S

T

P

G

I

I

K

L

L

L

M

R

D

D

Y

W

V

V

D

V

K

E

C

V

L

E

S

K

L

R

G

G

F

A

F

G

R

E

F

I

L

L

C

L

T

T

D

S

I

I

S

D

K

R

D
|
D

G
|
G

K

T

L

K

G

S

G

G

A

Y

A

D

I

T

D

E

L

M

Y

I

E

-

K

R

L

F

L

V

E

R

H

P

S

L

P

T

M

T

I

L

K

P

L

I

I

I

A

A

S

S

G

G

V

A

S

G

S

K

M

M

Q

E

D

H

I

F

Y

L

D

E

L

A

A

F

K

L

Y

A

P

G

I

A

R

D

S

A

V

A

V

L

V

A

G

A

K
x
S

A

V

I

F

Y

H

E

F

D

R

R

E

I

I

T

D

I

V

E

R

E

E

I

L

K

K

E

E

W

Y

active site: N11 (≠ D8), D130 (= D134)
binding phosphate ion: G82 (= G81), N103 (≠ G102), T104 (= T103), D176 (= D176), G177 (= G177), S225 (≠ K226)

7qc8A Imidazole glycerol phosphate synthase subunit HisF (see paper)
25% identity, 96% coverage: 3:241/249 of query aligns to 6:240/250 of 7qc8A

query
sites
7qc8A

I

I

P

A

A

A

I

L

D
x
E

I

V

L

K

D

D

G

G

K

R

V

V

R

K

L

-

R

-

E

-

G

G

D

T

Y

N

N

F

Q

E

K

N

T

L

E

R

Y

D

A

S

V

G

S

D

I

P

P

A

E

E

M

L

I

G

E

K

K

F

Y

Y

R

S

S

E

N

I

G

G

T

I

D

D

F

E

I

L

H

V

I

F

I
x
H

D

D

L
x
H

N

T

G

A

A

S

K

-

G

-

D

-

F

V

S

E

N

K

Q

R

K

K

T

T

L

M

F

L

E

E

I

L

I

V

K

E

K

K

T

V

E

A

M

E

K

Q

V

I

-

D

-

I

-

P

-

F

Q

T

Y

V

G

G

I

I

R

H

T

D

I

F

E

E

Q

T

V

A

T

S

N

E

L

L

L

I

D

L

A

R

G

G

I

A

H

D

R

K

V

V

I

S

V

I

G

N

T

T

Q

A

A

A

I

V

T

E

N

N

P

P

D

S

F

L

L

I

P

T

Q

Q

L

I

S

A

E

Q

A

T

F

F

A

G

K

-

D

-

Y

-

A

S

N

Q

R

A

I

V

V

V

I

V

A

A

I

I

D

D

V

A

-

K

-

R

L

V

D

D

E

G

-

E

-

F

-

M

V

V

I

F

K

T

Y

Y

S

S

G

G

W

-

M

K

E

K

S

N

S

T

P

G

I

I

K

L

L

L

M

R

D

D

Y

W

V

V

D

V

K

E

C

V

L

E

S

K

L

R

G

G

F

A

F

G

R

E

F

I

L

L

C

L

T

T

D

S

I

I

S

D

K

R

D

D

G

G

K

T

L

K

G

S

G

G

A

Y

A

D

I

T

D

E

L

M

Y

I

E

-

K

R

L

F

L

V

E

R

H

P

S

L

P

T

M

T

I

L

K

P

L

I

I

I

A

A

S

S

G

G

V

A

S

G

S

K

M

M

Q

E

D

H

I

F

Y

L

D

E

L

A

A

F

K

L

Y

A

P

G

I

A

R

D

S

A

V

A

V

L

V

A

G

A

K

S

A

V

I

F

Y

H

E

F

D

R

R

E

I

I

T

D

I

V

E

R

E

E

I

L

K

K

E

E

W

Y

binding zinc ion: E11 (≠ D8), H50 (≠ I50), H52 (≠ L52)

2wjzE Crystal structure of (hish) k181a y138a mutant of imidazoleglycerolphosphate synthase (hish hisf) which displays constitutive glutaminase activity (see paper)
25% identity, 96% coverage: 3:241/249 of query aligns to 6:226/237 of 2wjzE

query
sites
2wjzE

I

I

P

A

A

C

I

L

D
|
D

I

V

L

K

D

D

G

G

K

R

V

V

R

-

L

-

R

-

E

-

G

G

D

D

Y

-

N

-

Q

-

K

-

T

-

E

-

Y

-

A

-

V

-

S

-

I

-

P

P

E

V

M

E

I

L

E

G

K

K

Y

F

R

Y

S

S

N

E

-

I

G

G

T

I

D

D

F

E

I

L

H

V

I

F

I

L

D

D

L

I

N

T

G

-

A

-

K

-

G

-

D

-

F

-

S

A

N

K

Q

R

K

K

T

T

L

M

F

L

E

E

I

L

I

V

K

E

K

K

T

V

E

A

M

E

K

Q

V

I

-

D

-

I

-

P

-

F

Q

T

Y

V

G

G

G
|
G

I

I

R

H

T

D

I

F

E

E

Q

T

V

A

T

S

N

E

L

L

L

I

D

L

A

R

G

G

I

A

H

D

R

K

V

V

I

S

V

I

G
x
N

T
|
T

Q

A

A

A

I

V

T

E

N

N

P

P

D

S

F

L

L

I

P

T

Q

Q

L

I

S

A

E

Q

A

T

F

F

A

G

K

-

D

-

Y

-

A

S

N

Q

R

A

I

V

V

V

I

V

A

A

I

I

D
|
D

V

A

-

K

-

R

L

V

D

D

E

G

-

E

-

F

-

M

V

V

I

F

K

T

Y

Y

S

S

G

G

W

-

M

K

E

K

S

N

S

T

P

G

I

I

K

L

L

L

M

R

D

D

Y

W

V

V

D

V

K

E

C

V

L

E

S

K

L

R

G

G

F

A

F

G

R

E

F

I

L

L

C

L

T

T

D

S

I

I

S

D

K

R

D

D

G

G

K

T

L

K

G

S

G

G

A

Y

A

D

I

T

D

E

L

M

Y

I

E

-

K

R

L

F

L

V

E

R

H

P

S

L

P

T

M

T

I

L

K

P

L

I

I

I

A

A

S

S

G

G

V

A

S

G

S

K

M

M

Q

E

D

H

I

F

Y

L

D

E

L

A

A

F

K

L

Y

A

P

G

I

A

R

D

S

A

V

A

V

L

V

A

G

A

K

S

A

V

I

F

Y

H

E

F

D

R

R

E

I

I

T

D

I

V

E

R

E

E

I

L

K

K

E

E

W

Y

active site: D11 (= D8), D116 (= D134)
binding phosphate ion: G68 (= G81), N89 (≠ G102), T90 (= T103)

4ewnD Structure of hisf-d130v+d176v with bound rcdrp (see paper)
23% identity, 96% coverage: 3:241/249 of query aligns to 5:233/243 of 4ewnD

query
sites
4ewnD

I

I

P

A

A

C

I

L

D

D

I

V

L

K

D

D

G

G

K

R

V

V

R

K

L

-

R

-

E

-

G

G

D

T

Y

N

N

F

Q

E

K

N

T

L

E

R

Y

D

A

S

V

G

S

D

I

P

P

V

E

E

M

L

I

G

E

K

K

F

Y

Y

R

S

S

E

N

I

G

G

T

I

D

D

F

E

I

L

H

V

I

F

I

L

D

D

L

I

N

T

G

-

A

-

K

-

G

-

D

-

F

-

S

-

N

-

Q

-

K

K

T

T

L

M

F

L

E

E

I

L

I

V

K

E

K

K

T

V

E

A

M

E

K

Q

V

I

-

D

-

I

-

P

-

F

Q

T

Y

V

G

G

I

I

R

H

T

D

I

F

E

E

Q

T

V

A

T

S

N

E

L

L

L

I

D

L

A

R

G

G

I

A

H

D

R

K

V

V

I

S

V

I

G

N

T

T

Q

A

A

A

I

V

T

E

N

N

P

P

D

S

F

L

L

I

P

T

Q

Q

L

I

S

A

E

Q

A

T

F

F

A

G

K

S

K

Q

D

A

D

V

Y

V

A

V

N

A

R

I

I
x
V

V

A

I

K

A

R

I

V

D

D

V

G

L

E

D

F

E

M

V

V

I

F

K

T

Y

Y

S

S

G

G

W

-

M

K

E

K

S

N

S

T

P

G

I

I

K

L

L

L

M

R

D

D

Y

W

V

V

D

V

K

E

C

V

L

E

S

K

L

R

G

G

F

A

F

G

R

E

F

I

L

L

C

L

T

T

D

S

I

I

S

D

K

R

D

V

G
|
G

K

T

L

K

G

S

G

G

A

Y

A

D

I

T

D

E

L

M

Y

I

E

-

K

R

L

F

L

V

E

R

H

P

S

L

P

T

M

T

I

L

K

P

L

I

I

I

A

A

S
|
S

G
|
G

V

A

S

G

S

K

M

M

Q

E

D

H

I

F

Y

L

D

E

L

A

A

F

K

L

Y

A

P

G

I

A

R

D

S

A

V

A

V
x
L

V
x
A

G
x
A

K
x
S

A

V

I

F

Y

H

E

F

D

R

R

E

I

I

T

D

I

V

E

R

E

E

I

L

K

K

E

E

W

Y

binding 1-(O-carboxy-phenylamino)-1-deoxy-D-ribulose-5-phosphate: V123 (≠ I129), G170 (= G177), S194 (= S202), G195 (= G203), G196 (= G204), L215 (≠ V223), A216 (≠ V224), A217 (≠ G225), S218 (≠ K226)

P60664 Imidazole glycerol phosphate synthase subunit HisF; IGP synthase cyclase subunit; IGP synthase subunit HisF; ImGP synthase subunit HisF; IGPS subunit HisF; EC 4.3.2.10 from Escherichia coli (strain K12) (see paper)
23% identity, 83% coverage: 3:209/249 of query aligns to 6:214/258 of P60664

query
sites
P60664

I

I

P

P

A

C

I

L

D

D

I

V

L

R

D

D

G

G

K

Q

V

V

R

K

L

-

R

-

E

-

G

G

D

V

Y

Q

N

F

Q

R

K

N

T

H

E

E

Y

I

A

I

V

G

S

D

I

I

P

V

E

P

M

L

I

A

E

K

K

R

Y

Y

R

A

S

E

N

E

G

G

T

A

D

D

F
x
E

I

L

H

V

I

F

I

Y

D

D

L

I

N

T

G

A

A

S

K

S

-

D

G

G

D

R

F

V

S

V

N

D

Q

K

K

S

T

W

L

V

F

S

E

R

I

V

I

A

K

E

K

V

T

I

E

D

M

I

K

P

V

F

Q

C

Y

V

G

A

G

G

I

I

R

K

T

S

I

L

E

E

Q

D

V

A

T

A

N

K

L

I

L

L

D

S

A

F

G

G

I

A

H

D

R

K

V

I

I

S

V

I

G

N

T

S

Q

P

A

A

I

L

T

A

N

D

P

P

D

T

F

L

L

I

P

T

Q

R

L

L

S

A

E

D

A

R

F

F

A

G

K

-

D

-

Y

-

A

V

N
x
Q

R
x
C

I

I

V

V

I

V

A

G

I

I

D

D

V

T

L

W

D

Y

E

D

-

A

-

E

-

T

-

G

-

K

-

Y

-

H

V

V

I

N

K

Q

Y

Y

S

T

G

G

W

D

M

E

E

S

S

R

S

T

P

R

I

V

-

T

-

Q

-

W

K

E

L

T

M

L

D

D

Y

W

V

V

D

Q

K

E

C

V

L

Q

S

K

L

R

G

G

F

A

F

G

R

E

F

I

L

V

C

L

T

N

D

M

I

M

S

N

K

Q

D

D

G

G

K

V

L

R

G

N

G

G

A

Y

A

D

I

L

D

E

L

Q

Y

L

E

K

K

K

L

V

L

R

E

E