SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing CA265_RS04220 FitnessBrowser__Pedo557:CA265_RS04220 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P11170 Sodium/glucose cotransporter 1; Na(+)/glucose cotransporter 1; High affinity sodium-glucose cotransporter; Solute carrier family 5 member 1 from Oryctolagus cuniculus (Rabbit) (see 2 papers)
33% identity, 88% coverage: 3:486/550 of query aligns to 25:534/662 of P11170

query
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P11170

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C255 (vs. gap) modified: Disulfide link with 608
Q457 (≠ N418) mutation to W: Drasticly decreased affinity for glucose and phlorizin.
T460 (≠ I421) mutation to W: Decreased affinity for glucose and phlorizin.

Sites not aligning to the query:

608 modified: Disulfide link with 255

Q9NY91 Probable glucose sensor protein SLC5A4; Solute carrier family 5 member 4 from Homo sapiens (Human) (see paper)
31% identity, 85% coverage: 3:469/550 of query aligns to 25:508/659 of Q9NY91

query
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Q9NY91

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E457 (≠ N418) mutation to Q: Confers sugar transport activity not found in the wild-type protein. Increased sensitivity to inhibitor phlorizin.

7wmvA Structure of human sglt1-map17 complex bound with lx2761 (see paper)
33% identity, 90% coverage: 3:497/550 of query aligns to 8:528/602 of 7wmvA

query
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G

G

F

Y

I

L

K

K

I

L

L

M

P

P

V

M

F

F

I

I

F

M

V

V

L

M

P

P

G

G

L

M

F

I

A

S

Y

R

I

I

L

L

Y

Y

K

T

S

E

G

K

T

I

M

A

D

C

L

V

S

V

S

P

L

S

Q

E

T

C

V

E

G

K

S

Y

N

C

G

G

E

T

T

K

V

V

L

G

N

C

T

T

K

N

G

I

I

A

Y

Y

T

P

L

T

M

L

I

V

T

V

Q

E

L

L

L

M

P

P

K

N

G

G

L

L

V

R

G

G

I

L

L

M

V

L

A

S

A

V

L

M

L

L

S

A

G

S

L

L

M

M

S

S

Q

S

I

L

A

T

G

S

A

I

L

F

N

N

S

S

I

A

A

S

T

T

L

L

S

F

S

T

Y

M

D

D

L

I

Y

Y

K

A

R

K

F

V

K

R

P

K

E

R

T

A

S

S

D

E

K

K

K

E

L

L

V

M

S

I

V

A

G

G

R

R

W

L

S

F

A

I

G

L

I

V

A

L

L

I

T

G

V

I

S

S

I

I

G

A

L

W

L

V

P

P

L

I

L

V

N

Q

S

S

Y

A

E

Q

S

S

-

G

-

Q

L

L

F
|
F

N
x
D

G

Y

I

I

N
x
Q

D

S

V

I

I

T

A

S

H

Y

I

L

A

G

P

P

I

I

T

A

C

A

V

V

F

F

L

L

G

A

V

I

F

F

W

W

K

K

K

R

A

V

S

N

A

E

K

P

G

G

A

A

Q

F

Y

W

T

G

L

L

L

I

L

L

G

G

S

L

I

L

I

I

G

G

A

I

G

S

V

R

F

M

V

I

V

T

N

E

K

F

V

A

Y

Y

G

G

T

T

-

G

-

S

-

C

-

M

-

E

-

P

-

S

-

N

-

C

E

P

T

T

I

I

G

C

Q

G

I

V

P
x
H

F

Y

M

L

M

Y

M

F

A

A

F

I

Y

I

L

L

F

F

C

A

I

I

C

S

V

F

L

I

I

T

Q

I

V

V

F

I

S

S

binding N-[2-(dimethylamino)ethyl]-2-methyl-2-[4-[4-[[2-methyl-5-[(2S,3R,4R,5S,6R)-6-methylsulfanyl-3,4,5-tris(oxidanyl)oxan-2-yl]phenyl]methyl]phenyl]butanoylamino]propanamide: N61 (= N59), H66 (= H64), L70 (= L68), I81 (≠ N79), F84 (= F82), L257 (≠ M237), M266 (≠ Y246), L269 (= L249), T270 (≠ G250), Y273 (= Y253), W274 (= W254), F436 (= F414), D437 (≠ N415), Q440 (≠ N418), H508 (≠ P477)

P13866 Sodium/glucose cotransporter 1; Na(+)/glucose cotransporter 1; High affinity sodium-glucose cotransporter; Solute carrier family 5 member 1 from Homo sapiens (Human) (see 6 papers)
33% identity, 90% coverage: 3:497/550 of query aligns to 25:545/664 of P13866

query
sites
P13866

S

N

T

A

D

D

I

I

V

S

I

I

T

I

I

V

A

I

Y

Y

I

F

L

V

F

V

I

V

V

M

T

A

I

V

G

G

L

L

W

W

T

A

G

M

T

F

R

S

K

T

K
x
N

K

R

N

G

E

-

T

-

T

T

S

V

G

G

E

G

Y

F

F

F

L

L

A

A

G

G

K

R

T

S

L

M

K

V

W
|
W

P

W

M

P

I

I

G

G

L

A

A

S

L

L

F

F

A

A

T
x
S

N

N

I

I

S

G

C

S

L

G

H
|
H

L

F

V

V

S

G

L

L

A

A

Q

G

S

T

G

G

F

A

D

A

S

S

G

G

L

I

L

A

N

I

G

G

N

G

F

F

E

E

W

W

M

N

A

A

A

L

F

V

T

L

L

V

I

V

L

V

L

L

A

G

L

W

L

L

F

F

I

V

P

P

F

I

Y

Y

I

I

R

K

S

A

G

G

I

V

S

V

T

T

L

M

P

P

D

E

F

Y

L

L

E

R

R

K

R
|
R

Y

F

-

G

N

G

R

Q

A

R

C

I

R

Q

D

V

W

Y

L

L

A

S

F

L

I

L

S

S

I

L

L

L

S

L

A

Y

I

I

I

F

I

T

H

K

I

I

A
x
S

F

A

S

D

F

I

L

F

A

S

G

G

G
x
A

I

I

V

F

L

I

E

N

T

L

L

A

F

L

G

G

I

L

D

N

M

L

Y

Y

V

L

S

A

I

I

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F

V

L

I

L

A

L

L

A

L

I

T

T

G

A

L

L

Y
|
Y

T
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T

I
|
I

I
x
T

G
|
G

L
|
L

R
x
A

A
|
A

V
|
V

V
x
I

V
x
Y

T
|
T

E
x
D

T
|
T

I
x
L

Q
|
Q

S
x
T

L
x
V

V
x
I

L
x
M

I
x
L

T
x
V

G
|
G

A
x
S

I
x
L

I
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I

I
x
L

T
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T

Y
x
G

F
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F

A
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A

W
x
F

N
x
H

K
x
E

V
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V

G
|
G

W
x
Y

D
|
D

-
x
A

-
x
F

-
x
M

-
x
E

-
x
K

H
x
Y

M
|
M

T
x
K

A
|
A

-
x
I

-
x
P

-
x
T

I
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I

L
x
V

Q
x
S

K
x
D

E
x
G

N
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N

A
x
T

M
x
T

D
x
F

K
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Q

L
x
E

S
x
K

M
x
C

I
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Y

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x
T

P
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P

I
x
R

G
x
A

D
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D

K
x
S

-
x
F

-
x
H

-
x
I

-
x
F

-
x
R

-
x
D

-
x
P

-
x
L

-
x
T

S
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G

G
x
D

M
x
L

S
x
P

W
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W

I
x
P

A
x
G

V
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F

F
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I

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x
F

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G

Y
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M

P
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S

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x
I

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L

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T

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x
L

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W

Y
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Y

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W

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C

A
x
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D

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Q

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x
V

I
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I

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V

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Q

R
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R

V
x
C

L
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L

G
x
S

A
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A

K
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K

D
x
N

E
x
M

N
x
S

H
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H

A
x
V

R
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K

V
x
G

G
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G

S
x
C

L
x
I

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x
L

C
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C

G
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G

F
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Y

I
x
L

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K

I
x
L

L
x
M

P
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P

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x
M

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F

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I

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M

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V

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x
M

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P

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G

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M

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I

A
x
S

Y
x
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I
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I

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L

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Y

K
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E

G
x
K

T
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I

M
x
A

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C

L
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V

S
x
P

L
x
S

Q
x
E

T
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C

V
x
E

G
x
K

S
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Y

N
x
C

G
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G

E
x
T

T
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K

V
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V

L
x
G

N
x
C

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T

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N

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I
x
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Y

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P

L
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T

M
x
L

I
x
V

T
x
V

Q
x
E

L
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L

L
x
M

P
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P

K
x
N

G
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G

L
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L

V
x
R

G
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G

I
x
L

L
x
M

V
x
L

A
x
S

A
x
V

L
x
M

L
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L

S
x
A

G
x
S

L
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L

M
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M

S
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S

Q
x
S

I
x
L

A
x
T

G
x
S

A
x
I

L
x
F

N
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N

S
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S

I
x
A

A
x
S

T
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T

L
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L

S
x
F

S
x
T

Y
x
M

D
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D

L
x
I

Y
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Y

K
x
A

R
x
K

F
x
V

K
x
R

P
x
K

E
x
R

T
x
A

S
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S

D
x
E

K
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K

K
x
E

L
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L

V
x
M

S
x
I

V
x
A

G
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G

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R

W
x
L

S
x
F

A
x
I

G
x
L

I
x
V

A
x
L

L
x
I

T
x
G

V
x
I

S
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S

I
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I

G
x
A

L
x
W

L
x
V

P
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P

L
x
I

L
x
V

N
x
Q

S
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S

Y
x
A

E
x
Q

S
|
S

-
x
G

-
x
Q

L
|
L

F
|
F

N
x
D

G
x
Y

I
|
I

N
x
Q

D
x
S

V
x
I

I
x
T

A
x
S

H
x
Y

I
x
L

A
x
G

P
|
P

I
|
I

T
x
A

C
x
A

V
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V

F
|
F

L
|
L

G
x
A

V
x
I

F
|
F

W
|
W

K
|
K

K
x
R

A
x
V

S
x
N

A
x
E

K
x
P

G
|
G

A
|
A

Q
x
F

Y
x
W

T
x
G

L
|
L

L
x
I

L
|
L

G
|
G

S
x
L

I
x
L

I
|
I

G
|
G

A
x
I

G
x
S

V
x
R

F
x
M

V
x
I

V
x
T

N
x
E

K
x
F

V
x
A

Y
|
Y

G
|
G

T
|
T

-
x
G

-
x
S

-
x
C

-
x
M

-
x
E

-
x
P

-
x
S

-
x
N

-
x
C

E
x
P

T
|
T

I
|
I

G
x
C

Q
x
G

I
x
V

P
x
H

F
x
Y

M
x
L

M
x
Y

M
x
F

A
|
A

F
x
I

Y
x
I

L
|
L

F
|
F

C
x
A

I
|
I

C
x
S

V
x
F

L
x
I

I
x
T

Q
x
I

V
|
V

F
x
I

S
|
S

N51 (≠ K29) to S: in GGM; slightly decreased activity; dbSNP:rs17683011
W67 (= W48) mutation to A: Strong reduction in D-glucose transporter activity.
S77 (≠ T58) mutation to A: Loss of activity.
H83 (= H64) mutation to L: Acquires D-mannose, D-fructose and L-sorbose transporter activity; when associated with A-287 and C-290.; mutation to Q: Loss of D-glucose transporter activity.
R135 (= R116) to W: in GGM; loss of activity
S159 (≠ A139) to P: in GGM; loss of activity
A166 (≠ G146) to T: in GGM; about 90% reduction in activity
D204 (≠ E184) mutation to A: Loss of activity.
N248 (= N220) modified: carbohydrate, N-linked (GlcNAc...) asparagine; mutation to Q: Loss of N-glycosylation.
C255 (≠ M227) modified: Disulfide link with 511
W276 (= W239) to L: in GGM; about 95% reduction in activity
T287 (≠ G250) mutation to A: Acquires D-mannose, D-fructose and L-sorbose transporter activity; when associated with L-83 and C-290.; mutation to N: Loss of D-glucose transporter activity. Has strict selectivity for D-galactose.; mutation T->S,A: Has normal D-glucose and D-galactose transporter activity.
Y290 (= Y253) mutation to C: Loss of D-galactose transporter activity. Has strict selectivity for D-glucose. Acquires D-mannose, D-fructose and L-sorbose transporter activity; when associated with A-287 and L-83.
W291 (= W254) mutation to A: Loss of D-glucose transporter activity.
C292 (= C255) to Y: in GGM; loss of activity; mutation to A: Has no effect on water permeability.
Q295 (= Q258) to R: in GGM; loss of activity
R300 (= R263) to S: in GGM; loss of activity
A304 (= A267) to V: in GGM; impairs trafficking to the plasma membrane
K321 (= K284) mutation to Q: Acquires D-mannose and D-allose transporter activity comparable to glucose and galactose.
C345 (≠ D308) modified: Disulfide link with 351
C351 (≠ T314) modified: Disulfide link with 345
C355 (≠ N318) modified: Disulfide link with 361
C361 (≠ N324) modified: Disulfide link with 355
N363 (≠ K326) mutation to A: Loss of water permeation.
L369 (≠ M332) to S: in GGM; loss of activity
R379 (≠ V342) to Q: in GGM; loss of activity
A388 (≠ S351) to V: in GGM; loss of activity
S396 (≠ G359) mutation to A: Loss of activity.
F405 (≠ S368) to S: in GGM; loss of activity
A411 (≠ K374) to T: in GGM; slightly decreased activity; dbSNP:rs17683430
G426 (= G389) to R: in GGM; loss of activity
Q451 (vs. gap) mutation to A: Strong reduction in water permeation.
L452 (= L413) mutation to A: Loss of water permeation.
D454 (≠ N415) mutation to A: Has no effect on water permeation.
Q457 (≠ N418) mutation to A: Loss of D-glucose transporter activity.; mutation to C: Strong reduction in D-glucose transporter activity.
T460 (≠ I421) mutation to A: Loss of D-glucose transporter activity.
V470 (= V431) to N: in GGM; about 90% reduction in activity; requires 2 nucleotide substitutions
R499 (≠ V460) to H: in GGM; impairs trafficking to the plasma membrane; decreases the sugar affinity
C511 (vs. gap) modified: Disulfide link with 255
C517 (vs. gap) modified: Disulfide link with 522
C522 (≠ G474) modified: Disulfide link with 517

Sites not aligning to the query:

191:664 natural variant: Missing (in GGM; loss of activity)
379:664 natural variant: Missing (in GGM; loss of activity)
615 H → Q: in GGM; slightly decreased activity
641 W→A: Slightly reduced D-glucose transporter activity.
660:661 HA→WG: Loss of D-glucose transporter activity.

7slaA Cryoem structure of sglt1 at 3.15 angstrom resolution (see paper)
31% identity, 99% coverage: 3:549/550 of query aligns to 7:576/585 of 7slaA

query
sites
7slaA

S

N

T

A

D

D

I

I

V

S

I

V

T

I

I

V

A

I

Y

Y

I

F

L

L

F

L

I

V

V

M

T

A

I

V

G

G

L

L

W

W

T

S

G

-

T

-

R

-

K

-

N

-

E

-

T

-

S

-

G

-

E

-

Y

-

F

-

L

M

A

F

G

K

K

R

T

S

L

M

K

V

W
|
W

P

W

M

P

I

I

G

G

L

A

A

S

L

L

F

F

A

A

T

S

N

N

I

I

S

G

C

S

L

G

H

H

L

F

V

I

S

G

L

L

A

A

Q

G

S

T

G

G

F

A

D

A

S

S

G

G

L

L

L

A

N

V

G

G

N

G

F

F

E

E

W

W

M

N

A

A

A

L

F

V

T

L

L

L

I

L

L

V

L

L

A

G

L

W

L

V

F

F

I

V

P

P

F

I

Y

Y

I

I

R

K

S

A

G

G

I

V

S

V

T

T

L

M

P

P

D

E

F

Y

L

L

E

R

R

K

R

R

Y

F

N

G

-

G

R

Q

A

R

C

I

R

Q

D

V

W

Y

L

L

A

S

F

V

I

L

S

S

I

L

L

F

S

L

A

Y

I

I

I

F

I

T

H

K

I

I

A

S

F

V

S

D

F

I

L

F

A

S

G

G

G

A

I

I

V

F

L

I

E

N

T

L

L

A

F

L

G

G

I

W

D

N

M

L

Y

Y

V

L

S

S

I

I

V

I

V

L

I

L

A

L

L

A

L

I

T

T

G

A

L

L

Y

Y

T

T

I

I

I

T

G

G

L

L

R

A

A

A

V

V

V

I

V

Y

T

T

E

D

T

T

I

L

Q

Q

S

T

L

L

V

I

L

M

I

L

T

I

G

G

A

A

I

L

I

I

I

L

T

M

Y

G

F

F

A

A

W

F

N

H

K

E

V

V

G

G

W

Y

D

D

-

A

-

F

-

M

-

E

-

K

H

Y

M

M

T

K

A

A

I

I

-

P

-

T

-

I

L

V

Q

S

K

D

E

G

N

N

A

T

M

T

D

F

K

Q

L

E

S

K

M

C

I

Y

R

T

P

P

I

R

G

A

D

D

K

S

-

F

-

H

-

I

-

F

-

R

-

D

-

P

-

L

-

T

S

G

G

D

M

L

S

P

W

W

I

P

A

G

V

F

F

I

L

F

G

G

Y

L

P

T

V

I

L

L

G

A

I

L

W

W

Y

Y

W

W

C

C

A

T

D

D

Q

Q

T

V

I

I

V

V

Q

Q

R

R

V

C

L

L

G

A

A

A

K

K

D

N

E

M

N

S

H

H

A

V

R

K

V

G

G

G

S

C

L

I

F

L

C

A

G

G

F
x
Y

I

L

K

K

I

L

L

L

P

P

V

M

F

F

I

I

F

M

V

V

L

M

P

P

G

G

L

M

F

I

A

S

Y

R

I

I

L

L

Y

F

K

P

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D

G

K

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M

A

D

C

L

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S

V

S

P

L

S

Q

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V

E

G

K

S

Y

N

C

G

G

E

T

T

K

V

V

L

G

N

C

T

T

K

N

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I

I

A

Y

Y

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P

L

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M

L

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T

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Q

E

L

L

L

M

P

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N

G

G

L

L

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G

G

I

L

L

M

V

L

A

A

A

V

L

M

L

L

S

A

G

A

L

L

M

M

S

S

Q

S

I

L

A

T

G

S

A

I

L

F

N

N

S

S

I

A

A

S

T

T

L

L

S

F

S

T

Y

M

D

D

L

I

Y

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A

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K

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S

D

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K

K

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L

L

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G

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L

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F

A

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A

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L

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V

V

V

S

S

I

I

G

A

L

W

L

I

P

P

L

I

L

V

N

Q

S

S

Y

A

E

Q

S

S

-

G

-

Q

L

L

F

F

N

D

G

Y

I

I

N

Q

D

S

V

V

I

S

A

S

H

Y

I

L

A

A

P

P

I

V

T

A

C

A

V

V

F

F

L

L

G

A

V

I

F

F

W

W

K

K

K

R

A

V

S

N

A

E

K

Q

G

G

A

A

Q

F

Y

W

T

G

L

L

L

I

L

L

G

G

S

L

I

L

G

G

A

L

G

S

V

R

F

L

V

I

V

L

N

E

K

F

V

A

Y

Y

G

G

T

T

-

G

-

S

-

C

-

M

-

E

-

P

-

S

-

N

-

C

E

P

T

T

I

I

G

C

Q

G

I

V

P

H

F

Y

M

L

M

Y

M

F

A

A

F

I

Y

I

L

L

F

F

C

A

I

I

C

S

V

G

L

I

I

V

Q

T

V

V

F

V

S

S

-

L

-

T

-

K

-

P

-

I

-

P

-

D

-

V

H

H

I

L

Y

Y

P

R

V

L

K

C

-

W

-

S

-

L

H

R

T

N

A

S

Q

K

S

E

E

E

T

R

L

I

Y

D

W

L

T

M

S

K

I

M

W

T

E

D

P

T

L

S

K

E

S

K

K

P

G

L

W
|
W

S

R

G

T

I

V

G

L

N

N

Y

I

K

N

F

-

L

-

S

A

V

I

L

L

A

A

I

V

M

A

G

I

V

F

L

C

Y

H

V

A

Y

Y

F

F

binding cholesterol hemisuccinate: W36 (= W48), Y288 (≠ F282), W554 (= W525)

7sl8A Cryoem structure of sglt1 at 3.4 a resolution (see paper)
31% identity, 99% coverage: 3:549/550 of query aligns to 6:573/582 of 7sl8A

query
sites
7sl8A

S

N

T

A

D

D

I

I

V

S

I

V

T

I

I

V

A

I

Y

Y

I

F

L

L

F

L

I

V

V

M

T

A

I

V

G

G

L

L

W

W

T

S

G

-

T

-

R

-

K

-

N

-

E

-

T

-

S

-

G

-

E

-

Y

-

F

-

L

M

A

F

G

K

K

R

T

S

L

M

K

V

W
|
W

P

W

M

P

I

I

G

G

L

A

A

S

L

L

F

F

A

A

T

S

N

N

I

I

S

G

C

S

L

G

H

H

L

F

V

I

S

G

L

L

A

A

Q

G

S

T

G

G

F

A

D

A

S

S

G

G

L

L

L

A

N

V

G

G

N

G

F

F

E

E

W

W

M

N

A

A

A

L

F

V

T

L

L

L

I

L

L

V

L

L

A

G

L

W

L

V

F

F

I

V

P

P

F

I

Y

Y

I

I

R

K

S

A

G

G

I

V

S

V

T

T

L

M

P

P

D

E

F

Y

L

L

E

R

R

K

R

R

Y

F

N

G

-

G

R

Q

A

R

C

I

R

Q

D

V

W

Y

L

L

A

S

F

V

I

L

S

S

I

L

L

F

S

L

A

Y

I

I

I

F

I

T

H

K

I

I

A

S

F

V

S

D

F

I

L

F

A

S

G

G

G

A

I

I

V

F

L

I

E

N

T

L

L

A

F

L

G

G

I

W

D

N

M

L

Y

Y

V

L

S

S

I

I

V

I

V

L

I

L

A

L

L

A

L

I

T

T

G

A

L

L

Y

Y

T

T

I

I

I

T

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G

L

L

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A

A

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V

V

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V

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T

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L

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Q

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M

I

L

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G

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A

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F

A

A

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F

N

H

K

E

V

V

G

G

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D

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A

-

F

-

M

-

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K

H

Y

M

M

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K

A

A

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I

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P

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T

-

I

L

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Q

S

K

D

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G

N

N

A

T

M

T

D

F

K

Q

L

E

S

K

M

C

I

Y

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P

P

I

R

G

A

D

D

K

S

-

F

-

H

-

I

-

F

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D

-

P

-

L

-

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S

G

G

D

M

L

S

P

W

W

I

P

A

G

V

F

F

I

L

F

G

G

Y

L

P

T

V

I

L

L

G

A

I

L

W

W

Y

Y

W

W

C

C

A

T

D

D

Q

Q

T

V

I

I

V

V

Q

Q

R

R

V

C

L

L

G

A

A

A

K

K

D

N

E

M

N

S

H

H

A

V

R

K

V
x
G

G

G

S

C

L

I

F

L

C

A

G

G

F
x
Y

I

L

K

K

I

L

L

L

P

P

V

M

F

F

I

I

F

M

V

V

L

M

P

P

G

G

L

M

F

I

A

S

Y

R

I

I

L

L

Y

F

K

P

S

D

G

K

T

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M

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C

L

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S

V

S

P

L

S

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C

V

E

G

K

S

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N

C

G

G

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T

K

V

V

L

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N

C

T

T

K

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I

I

A

Y

Y

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P

L

T

M

L

I

V

T

V

Q

E

L

L

L

M

P

P

K

N

G

G

L

L

V

R

G

G

I

L

L

M

V

L

A

A

A

V

L

M

L

L

S

A

G

A

L

L

M

M

S

S

Q

S

I

L

A

T

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S

A

I

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F

N

N

S

S

I

A

A

S

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T

L

L

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M

D

D

L

I

Y

Y

K

A

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V

K

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A

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S

D

E

K

K

K

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L

L

V

M

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G

R

R

W

L

S

F

A

V

G

L

I

F

A

L

L

V

T

V

V

V

S

S

I

I

G

A

L

W

L

I

P

P

L

I

L

V

N

Q

S

S

Y

A

E

Q

S

S

-

G

-

Q

L

L

F

F

N

D

G

Y

I

I

N

Q

D

S

V

V

I

S

A

S

H

Y

I

L

A

A

P

P

I

V

T

A

C

A

V

V

F

F

L

L

G

A

V

I

F

F

W

W

K

K

K

R

A

V

S

N

A

E

K

Q

G

G

A

A

Q

F

Y

W

T

G

L

L

L

I

L

L

G

G

S

L

I

L

G

G

A

L

G

S

V

R

F

L

V

I

V

L

N

E

K

F

V

A

Y

Y

G

G

T

T

-

G

-

S

-

C

-

M

-

E

-

P

-

S

-

N

-

C

E

P

T

T

I

I

G

C

Q

G

I

V

P

H

F

Y

M

L

M

Y

M

F

A

A

F

I

Y

I

L

L

F

F

C

A

I

I

C

S

V

G

L

I

I

V

Q

T

V

V

F

V

S

S

-

L

-

T

-

K

-

P

-

I

-

P

-

D

-

V

H

H

I

L

Y

Y

-

R

-

L

-

C

-

W

P

S

V

L

K

R

H

N

T

S

A

K

Q

E

S

E

E

R

T

I

L

M

Y

K

W

M

T

T

S

D

I

T

W

S

E

E

-

K

P

P

L

L

K

-

S

-

K

-

G

-

W
|
W

S

R

G

T

I

V

G

L

N

N

Y

I

K
x
N

F

-

L

-

S

A

V

I

L

L

A

A

I

V

M

A

G

I

V

F

L

C

Y

W

V

G

Y

Y

F

F

binding cholesterol: W35 (= W48), G280 (≠ V275), Y287 (≠ F282), W551 (= W525), N558 (≠ K532)

8hezA Structure of human sglt2-map17 complex with dapagliflozin (see paper)
33% identity, 81% coverage: 3:446/550 of query aligns to 2:465/582 of 8hezA

query
sites
8hezA

S

N

T

P

T

A

D

D

I

I

V

L

I

V

T

I

I

A

A

A

Y

Y

I

F

L

L

F

L

I

V

V

I

T

G

I

V

G

G

L

L

W

W

T

S

G

M

T

C

R

R

K

T

K

N

K

R

N

G

E

-

T

-

T

T

S

V

G

G

E

G

Y

Y

F

F

L

L

A

A

G

G

K

R

T

S

L

M

K

V

W

W

P

W

M

P

I

V

G

G

L

A

A

S

L

L

F

F

A
|
A

T

S

N
|
N

I
|
I

S
x
G

C

S

L
x
G

H
|
H

L

F

V

V

S
x
G

L
|
L

A

A

Q

G

S
x
T

G

G

F

A

D

A

S

S

G

G

L

L

L

A

N

V

G

A

N

G

F
|
F

E
|
E

W

W

M

N

A

A

A

L

F

F

T

V

L

V

I

L

L

L

A

G

L

W

L

L

F

F

I

A

P

P

F

V

Y

Y

I

L

R

T

S

A

G

G

I

V

S

I

T

T

L

M

P

P

D

Q

F

Y

L

L

E

R

R

K

R

R

Y

F

-

G

N

G

R

R

A

R

C

I

R

R

D

L

W

Y

L

L

A

S

F

V

I

L

S

S

I

L

L

F

S

L

A

Y

I

I

I

F

I

T

H

K

I

I

A

S

F

V

S

D

F

M

L

F

A

S

G

G

G

A

I

V

V

F

L

I

E

Q

T

Q

L

A

F

L

G

G

I

W

D

N

M

I

Y

Y

V

A

S

S

I

V

V

I

V

A

I

L

A

L

L

G

L

I

T

T

G

M

L

I

Y

Y

T

T

I

V

I

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G

L

L

R

A

A

A

V

L

V

M

V

Y

T

T

E

D

T

T

I

V

Q

Q

S

T

L

F

V

V

L

I

I

L

T

G

G

G

A

A

I

C

I

I

I

L

T

M

Y

G

F

Y

A

A

W

F

N

H

K

E

V

V

G

G

W

Y

D

S

H

G

M

L

-

F

-

D

-

K

-

Y

-

L

-

G

-

A

-

T

T

S

A

L

I

T

L

V

Q

S

K

E

E

D

N

P

A

A

M

V

D

G

K

N

L

I

S

S

M

S

I

F

-

C

-

Y

R

R

P

P

I

R

G

P

D

D

K

S

-

Y

-

H

-

L

-

R

-

H

-

P

-

V

-

T

S

G

G

D

M

L

S

P

W

W

I

P

A

A

V

L

F

L

L

L

G

G

Y

L

P

T

V

I

L
x
V

G
x
S

I

G

W

W

Y

Y

W
|
W

C

C

A

S

D

D

Q

Q

T

V

I

I

V

V

Q

Q

R

R

V

C

L

L

G

A

A

G

K

K

D

S

E

L

N

T

H

H

A

I

R

K

V

A

G

G

S

C

L

I

F

L

C

C

G

G

F

Y

I

L

K
|
K

I

L

L

T

P

P

V

M

F

F

I

L

F

M

V

V

L

M

P

P

G

G

L

M

F

I

A

S

Y

R

I

I

L

L

Y

Y

K

P

S

D

G

E

T

V

M

A

D

C

L

V

S

V

S

P

L

E

Q

V

T

C

V

R

G

R

S

V

N

C

G

G

E

T

T

E

V

V

L

G

N

C

T

S

K

N

G

I

I

A

Y

Y

T

P

L

R

M

L

I

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T

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K

L

L

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M

P

P

K

N

G

G

L

L

V

R

G

G

I

L

L

M

V

L

A

A

A

V

L

M

L

L

S

A

G
x
A

L

L

M

M

S
|
S

Q
x
S

I

L

A

A

G

S

A

I

L

F

N

N

S

S

I

S

A

S

T

T

L

L

S

F

S

T

Y

M

D

D

L

I

Y

Y

K

T

R

R

F

L

K

R

P

P

E

R

T

A

S

G

D

D

K

R

K

E

L

L

V

L

S

L

V

V

G

G

R

R

W

L

S

W

A

V

G

V

I

F

A

I

L

V

T

V

V

V

S

S

I

V

G

A

L

W

L

L

P

P

L

V

N

Q

S

A

Y

A

E

Q

-

G

S

Q

L

L

F
|
F

N

D

G

Y

I

I

N
x
Q

D

A

V

V

I

S

A

S

H

Y

I

L

A

A

P

P

I

V

T

S

C

A

V

V

F

F

L

V

L

L

G

A

V

L

F

F

W

V

K

P

K

R

A

V

S

N

A

E

K

Q

G

G

A

A

binding (2S,3R,4R,5S,6R)-2-[4-chloranyl-3-[(4-ethoxyphenyl)methyl]phenyl]-6-(hydroxymethyl)oxane-3,4,5-triol: N55 (= N59), G59 (≠ L63), H60 (= H64), G63 (≠ S67), L64 (= L68), T67 (≠ S71), F78 (= F82), E79 (= E83), V266 (≠ L249), S267 (≠ G250), W271 (= W254), K301 (= K284), F433 (= F414), Q437 (≠ N418)
binding sodium ion: A53 (= A57), I56 (= I60), G57 (≠ S61), A369 (≠ G352), S372 (= S355), S373 (≠ Q356)

Sites not aligning to the query:

binding : 568, 571, 572, 575, 576, 579, 580

8hdhA Structure of human sglt2-map17 complex with canagliflozin (see paper)
33% identity, 81% coverage: 3:446/550 of query aligns to 2:465/586 of 8hdhA

query
sites
8hdhA

S

N

T

P

T

A

D

D

I

I

V

L

I

V

T

I

I

A

A

A

Y

Y

I

F

L

L

F

L

I

V

V

I

T

G

I

V

G

G

L

L

W

W

T

S

G

M

T

C

R

R

K

T

K

N

K

R

N

G

E

-

T

-

T

T

S

V

G

G

E

G

Y

Y

F

F

L

L

A

A

G

G

K

R

T

S

L

M

K

V

W

W

P

W

M

P

I

V

G

G

L

A

A

S

L

L

F

F

A
|
A

T
x
S

N
|
N

I
|
I

S
x
G

C

S

L
x
G

H
|
H

L

F

V

V

S
x
G

L
|
L

A

A

Q

G

S

T

G

G

F

A

D

A

S

S

G

G

L

L

L

A

N

V

G

A

N

G

F
|
F

E
|
E

W

W

M

N

A

A

A

L

F

F

T

V

L

V

I

L

L

L

A

G

L

W

L

L

F

F

I

A

P

P

F

V

Y

Y

I

L

R

T

S

A

G

G

I

V

S

I

T

T

L

M

P

P

D

Q

F

Y

L

L

E

R

R

K

R

R

Y

F

-

G

N

G

R

R

A

R

C

I

R

R

D

L

W

Y

L

L

A

S

F

V

I

L

S

S

I

L

L

F

S

L

A

Y

I

I

I

F

I

T

H

K

I

I

A

S

F

V

S

D

F

M

L

F

A

S

G

G

G

A

I

V

V

F

L

I

E

Q

T

Q

L

A

F

L

G

G

I

W

D

N

M

I

Y

Y

V

A

S

S

I

V

V

I

V

A

I

L

A

L

L

G

L

I

T

T

G

M

L

I

Y

Y

T

T

I

V

I

T

G

G

L

L

R

A

A

A

V

L

V

M

V

Y

T

T

E

D

T

T

I

V

Q

Q

S

T

L

F

V

V

L

I

I

L

T

G

G

G

A

A

I

C

I

I

I

L

T

M

Y

G

F

Y

A

A

W

F

N

H

K

E

V

V

G

G

W

Y

D

S

H

G

M

L

-

F

-

D

-

K

-

Y

-

L

-

G

-

A

-

T

T

S

A

L

I

T

L

V

Q

S

K

E

E

D

N

P

A

A

M

V

D

G

K

N

L

I

S

S

M

S

I

F

-

C

-

Y

R

R

P

P

I

R

G

P

D

D

K

S

-

Y

-

H

-

L

-

R

-

H

-

P

-

V

-

T

S

G

G

D

M

L

S

P

W

W

I

P

A

A

V

L

F

L

L

L

G

G

Y

L

P

T

V

I

L

V

G
x
S

I

G

W

W

Y

Y

W
|
W

C

C

A

S

D

D

Q

Q

T

V

I

I

V

V

Q

Q

R

R

V

C

L

L

G

A

A

G

K

K

D

S

E

L

N

T

H

H

A

I

R

K

V

A

G

G

S

C

L

I

F

L

C

C

G

G

F

Y

I

L

K

K

I

L

L

T

P

P

V

M

F

F

I

L

F

M

V

V

L

M

P

P

G

G

L

M

F

I

A

S

Y

R

I

I

L

L

Y

Y

K

P

S

D

G

E

T

V

M

A

D

C

L

V

S

V

S

P

L

E

Q

V

T

C

V

R

G

R

S

V

N

C

G

G

E

T

T

E

V

V

L

G

N

C

T

S

K

N

G

I

I

A

Y

Y

T

P

L

R

M

L

I

V

T

V

Q

K

L

L

L

M

P

P

K

N

G

G

L

L

V

R

G

G

I

L

L

M

V

L

A

A

A

V

L

M

L

L

S

A

G
x
A

L

L

M

M

S
|
S

Q
x
S

I

L

A

A

G

S

A

I

L

F

N

N

S

S

I

S

A

S

T

T

L

L

S

F

S

T

Y

M

D

D

L

I

Y

Y

K

T

R

R

F

L

K

R

P

P

E

R

T

A

S

G

D

D

K

R

K

E

L

L

V

L

S

L

V

V

G

G

R

R

W

L

S

W

A

V

G

V

I

F

A

I

L

V

T

V

V

V

S

S

I

V

G

A

L

W

L

L

P

P

L

V

N

Q

S

A

Y

A

E

Q

-

G

S

Q

L

L

F
|
F

N
x
D

G

Y

I

I

N
x
Q

D

A

V

V

I

S

A

S

H

Y

I

L

A

A

P

P

I

V

T

S

C

A

V

V

F

F

L

V

L

L

G

A

V

L

F

F

W

V

K

P

K

R

A

V

S

N

A

E

K

Q

G

G

A

A

binding (2~{S},3~{R},4~{R},5~{S},6~{R})-2-[3-[[5-(4-fluorophenyl)thiophen-2-yl]methyl]-4-methyl-phenyl]-6-(hydroxymethyl)oxane-3,4,5-triol: N55 (= N59), G59 (≠ L63), H60 (= H64), G63 (≠ S67), L64 (= L68), F78 (= F82), E79 (= E83), S267 (≠ G250), W271 (= W254), F433 (= F414), D434 (≠ N415), Q437 (≠ N418)
binding sodium ion: A53 (= A57), S54 (≠ T58), I56 (= I60), G57 (≠ S61), A369 (≠ G352), S372 (= S355), S373 (≠ Q356)

Sites not aligning to the query:

8hb0A Structure of human sglt2-map17 complex with ta1887 (see paper)
33% identity, 81% coverage: 3:446/550 of query aligns to 2:465/586 of 8hb0A

query
sites
8hb0A

S

N

T

P

T

A

D

D

I

I

V

L

I

V

T

I

I

A

A

A

Y

Y

I

F

L

L

F

L

I

V

V

I

T

G

I

V

G

G

L

L

W

W

T

S

G

M

T

C

R

R

K

T

K

N

K

R

N

G

E

-

T

-

T

T

S

V

G

G

E

G

Y

Y

F

F

L

L

A

A

G

G

K

R

T

S

L

M

K

V

W

W

P

W

M

P

I

V

G

G

L

A

A

S

L

L

F

F

A
|
A

T

S

N
|
N

I
|
I

S
x
G

C

S

L

G

H
|
H

L

F

V

V

S
x
G

L
|
L

A

A

Q

G

S
x
T

G

G

F

A

D

A

S

S

G

G

L

L

L

A

N
x
V

G

A

N

G

F
|
F

E
|
E

W

W

M

N

A

A

A

L

F

F

T

V

L

V

I

L

L

L

A

G

L

W

L

L

F

F

I

A

P

P

F

V

Y

Y

I

L

R

T

S

A

G

G

I

V

S

I

T

T

L

M

P

P

D

Q

F

Y

L

L

E

R

R

K

R

R

Y

F

-

G

N

G

R

R

A

R

C

I

R

R

D

L

W

Y

L

L

A

S

F

V

I

L

S

S

I

L

L

F

S

L

A

Y

I

I

I

F

I

T

H

K

I

I

A

S

F
x
V

S

D

F

M

L

F

A

S

G

G

G

A

I

V

V

F

L

I

E

Q

T

Q

L

A

F

L

G

G

I

W

D

N

M

I

Y

Y

V

A

S

S

I

V

V

I

V

A

I

L

A

L

L

G

L

I

T

T

G

M

L

I

Y

Y

T

T

I

V

I

T

G

G

L

L

R

A

A

A

V

L

V

M

V

Y

T

T

E

D

T

T

I

V

Q

Q

S

T

L

F

V

V

L

I

I

L

T

G

G

G

A

A

I

C

I

I

I

L

T

M

Y

G

F

Y

A

A

W

F

N

H

K

E

V

V

G

G

W

Y

D

S

H

G

M

L

-

F

-

D

-

K

-

Y

-

L

-

G

-

A

-

T

T

S

A

L

I

T

L

V

Q

S

K

E

E

D

N

P

A

A

M

V

D

G

K

N

L

I

S

S

M

S

I

F

-

C

-

Y

R

R

P

P

I

R

G

P

D

D

K

S

-

Y

-

H

-

L

-

R

-

H

-

P

-

V

-

T

S

G

G

D

M

L

S

P

W

W

I

P

A

A

V

L

F

L

L

L

G

G

Y

L

P

T

V

I

L
x
V

G
x
S

I

G

W

W

Y

Y

W
|
W

C

C

A

S

D

D

Q

Q

T

V

I

I

V

V

Q

Q

R

R

V

C

L

L

G

A

A

G

K

K

D

S

E

L

N

T

H

H

A

I

R

K

V

A

G

G

S

C

L

I

F

L

C

C

G

G

F

Y

I

L

K

K

I

L

L

T

P

P

V

M

F

F

I

L

F

M

V

V

L

M

P

P

G

G

L

M

F

I

A

S

Y

R

I

I

L

L

Y

Y

K

P

S

D

G

E

T

V

M

A

D

C

L

V

S

V

S

P

L

E

Q

V

T

C

V

R

G

R

S

V

N

C

G

G

E

T

T

E

V

V

L

G

N

C

T

S

K

N

G

I

I

A

Y

Y

T

P

L

R

M

L

I

V

T

V

Q

K

L

L

L

M

P

P

K

N

G

G

L

L

V

R

G

G

I

L

L

M

V

L

A

A

A

V

L

M

L

L

S

A

G
x
A

L

L

M

M

S
|
S

Q
x
S

I

L

A

A

G

S

A

I

L

F

N

N

S

S

I

S

A

S

T

T

L

L

S

F

S

T

Y

M

D

D

L

I

Y

Y

K

T

R

R

F

L

K

R

P

P

E

R

T

A

S

G

D

D

K

R

K

E

L

L

V

L

S

L

V

V

G

G

R

R

W

L

S

W

A

V

G

V

I

F

A

I

L

V

T

V

V

V

S

S

I

V

G

A

L

W

L

L

P

P

L

V

N

Q

S

A

Y

A

E

Q

-

G

S

Q

L

L

F
|
F

N

D

G

Y

I

I

N
x
Q

D

A

V

V

I

S

A

S

H

Y

I

L

A

A

P

P

I

V

T

S

C

A

V

V

F

F

L

V

L

L

G

A

V

L

F

F

W

V

K

P

K

R

A

V

S

N

A

E

K

Q

G

G

A

A

binding (2R,3R,4S,5S,6R)-2-[3-[(4-cyclopropylphenyl)methyl]-4-fluoranyl-indol-1-yl]-6-(hydroxymethyl)oxane-3,4,5-triol: N55 (= N59), H60 (= H64), G63 (≠ S67), L64 (= L68), T67 (≠ S71), V75 (≠ N79), F78 (= F82), E79 (= E83), V137 (≠ F140), V266 (≠ L249), S267 (≠ G250), W271 (= W254), F433 (= F414), Q437 (≠ N418)
binding sodium ion: A53 (= A57), I56 (= I60), G57 (≠ S61), A369 (≠ G352), S372 (= S355), S373 (≠ Q356)

Sites not aligning to the query:

binding : 575, 576, 579, 580, 583, 584

7vsiA Structure of human sglt2-map17 complex bound with empagliflozin (see paper)
33% identity, 81% coverage: 3:446/550 of query aligns to 2:465/586 of 7vsiA

query
sites
7vsiA

S

N

T

P

T

A

D

D

I

I

V

L

I

V

T

I

I

A

A

A

Y

Y

I

F

L

L

F

L

I

V

V

I

T

G

I

V

G

G

L

L

W

W

T

S

G

M

T

C

R

R

K

T

K

N

K

R

N

G

E

-

T

-

T

T

S

V

G

G

E

G

Y

Y

F

F

L

L

A

A

G

G

K

R

T

S

L

M

K

V

W

W

P

W

M

P

I

V

G

G

L

A

A

S

L

L

F

F

A

A

T

S

N
|
N

I

I

S

G

C

S

L

G

H
|
H

L

F

V

V

S
x
G

L
|
L

A

A

Q

G

S

T

G

G

F

A

D

A

S

S

G

G

L

L

L

A

N
x
V

G

A

N

G

F
|
F

E
|
E

W

W

M

N

A

A

A

L

F

F

T

V

L

V

I

L

L

L

A

G

L

W

L

L

F

F

I

A

P

P

F

V

Y

Y

I

L

R

T

S

A

G

G

I

V

S

I

T

T

L

M

P

P

D

Q

F

Y

L

L

E

R

R

K

R

R

Y

F

-

G

N

G

R

R

A

R

C

I

R

R

D

L

W

Y

L

L

A

S

F

V

I

L

S

S

I

L

L

F

S

L

A

Y

I

I

I

F

I

T

H

K

I

I

A

S

F

V

S

D

F

M

L

F

A

S

G

G

G

A

I

V

V

F

L

I

E

Q

T

Q

L

A

F

L

G

G

I

W

D

N

M

I

Y

Y

V

A

S

S

I

V

V

I

V

A

I

L

A

L

L

G

L

I

T

T

G

M

L

I

Y

Y

T

T

I

V

I

T

G

G

L

L

R

A

A

A

V

L

V

M

V

Y

T

T

E

D

T

T

I

V

Q

Q

S

T

L

F

V

V

L

I

I

L

T

G

G

G

A

A

I

C

I

I

I

L

T

M

Y

G

F

Y

A

A

W

F

N

H

K

E

V

V

G

G

W

Y

D

S

H

G

M

L

-

F

-

D

-

K

-

Y

-

L

-

G

-

A

-

T

T

S

A

L

I

T

L

V

Q

S

K

E

E

D

N

P

A

A

M

V

D

G

K

N

L

I

S

S

M

S

I

F

-

C

-

Y

R

R

P

P

I

R

G

P

D

D

K

S

-

Y

-

H

-

L

-

R

-

H

-

P

-

V

-

T

S

G

G

D

M

L

S

P

W

W

I

P

A

A

V

L

F

L

L

L

G

G

Y

L

P

T

V

I

L
x
V

G
x
S

I

G

W

W

Y
|
Y

W

W

C

C

A

S

D

D

Q

Q

T

V

I

I

V

V

Q

Q

R

R

V

C

L

L

G

A

A

G

K

K

D

S

E

L

N

T

H

H

A

I

R

K

V

A

G

G

S

C

L

I

F

L

C

C

G

G

F

Y

I

L

K

K

I

L

L

T

P

P

V

M

F

F

I

L

F

M

V

V

L

M

P

P

G

G

L

M

F

I

A

S

Y

R

I

I

L

L

Y

Y

K

P

S

D

G

E

T

V

M

A

D

C

L

V

S

V

S

P

L

E

Q

V

T

C

V

R

G

R

S

V

N

C

G

G

E

T

T

E

V

V

L

G

N

C

T

S

K

N

G

I

I

A

Y

Y

T

P

L

R

M

L

I

V

T

V

Q

K

L

L

L

M

P

P

K

N

G

G

L

L

V

R

G

G

I

L

L

M

V

L

A

A

A

V

L

M

L

L

S

A

G

A

L

L

M

M

S

S

Q

S

I

L

A

A

G

S

A

I

L

F

N

N

S

S

I

S

A

S

T

T

L

L

S

F

S

T

Y

M

D

D

L

I

Y

Y

K

T

R

R

F

L

K

R

P

P

E

R

T

A

S

G

D

D

K

R

K

E

L

L

V

L

S

L

V

V

G

G

R

R

W

L

S

W

A

V

G

V

I

F

A

I

L

V

T

V

V

V

S

S

I

V

G

A

L

W

L

L

P

P

L

V

N

Q

S

A

Y

A

E

Q

-

G

S

Q

L

L

F
|
F

N
x
D

G

Y

I

I

N
x
Q

D

A

V

V

I

S

A

S

H

Y

I

L

A

A

P

P

I

V

T

S

C

A

V

V

F

F

L

V

L

L

G

A

V

L

F

F

W

V

K

P

K

R

A

V

S

N

A

E

K

Q

G

G

A

A

binding (2S,3R,4R,5S,6R)-2-[4-chloranyl-3-[[4-[(3S)-oxolan-3-yl]oxyphenyl]methyl]phenyl]-6-(hydroxymethyl)oxane-3,4,5-triol: N55 (= N59), H60 (= H64), G63 (≠ S67), L64 (= L68), V75 (≠ N79), F78 (= F82), E79 (= E83), V266 (≠ L249), S267 (≠ G250), Y270 (= Y253), F433 (= F414), D434 (≠ N415), Q437 (≠ N418)

P31639 Sodium/glucose cotransporter 2; Na(+)/glucose cotransporter 2; Low affinity sodium-glucose cotransporter; Solute carrier family 5 member 2 from Homo sapiens (Human) (see paper)
33% identity, 81% coverage: 3:446/550 of query aligns to 22:485/672 of P31639

query
sites
P31639

S

N

T

P

T

A

D

D

I

I

V

L

I

V

T

I

I

A

A

A

Y

Y

I

F

L

L

F

L

I

V

V

I

T

G

I

V

G

G

L

L

W

W

T

S

G

M

T

C

R

R

K

T

K

N

K

R

N

G

E

-

T

-

T

T

S

V

G

G

E

G

Y

Y

F

F

L

L

A

A

G

G

K

R

T

S

L

M

K

V

W

W

P

W

M

P

I

V

G

G

L

A

A

S

L

L

F

F

A

A

T

S

N

N

I

I

S

G

C

S

L

G

H

H

L

F

V

V

S

G

L

L

A

A

Q

G

S

T

G

G

F

A

D

A

S

S

G

G

L

L

L

A

N
x
V

G

A

N

G

F
|
F

E

E

W

W

M

N

A

A

A

L

F

F

T

V

L

V

I

L

L

L

A

G

L

W

L

L

F

F

I

A

P

P

F

V

Y

Y

I

L

R

T

S

A

G

G

I

V

S

I

T

T

L

M

P

P

D

Q

F

Y

L

L

E

R

R

K

R

R

Y

F

-

G

N

G

R

R

A

R

C

I

R

R

D

L

W

Y

L

L

A

S

F

V

I

L

S

S

I

L

L

F

S

L

A

Y

I

I

I

F

I

T

H

K

I

I

A

S

F
x
V

S

D

F

M

L

F

A

S

G

G

G

A

I

V

V

F

L

I

E

Q

T

Q

L

A

F

L

G

G

I

W

D

N

M

I

Y

Y

V

A

S

S

I

V

V

I

V

A

I

L

A

L

L

G

L

I

T

T

G

M

L

I

Y

Y

T

T

I

V

I

T

G

G

L

L

R

A

A

A

V

L

V

M

V

Y

T

T

E

D

T

T

I

V

Q

Q

S

T

L

F

V

V

L

I

I

L

T

G

G

G

A

A

I

C

I

I

I

L

T

M

Y

G

F

Y

A

A

W

F

N

H

K

E

V

V

G

G

W

Y

D

S

H

G

M

L

-

F

-

D

-

K

-

Y

-

L

-

G

-

A

-

T

T

S

A

L

I

T

L

V

Q

S

K

E

E

D

N

P

A

A

M

V

D

G

K

N

L

I

S

S

M

S

I

F

-

C

-

Y

R

R

P

P

I

R

G

P

D

D

K

S

-

Y

-

H

-

L

-

R

-

H

-

P

-

V

-

T

S

G

G

D

M

L

S

P

W

W

I

P

A

A

V

L

F

L

L

L

G

G

Y
x
L

P

T

V

I

L

V

G

S

I

G

W

W

Y

Y

W

W

C

C

A

S

D

D

Q

Q

T

V

I

I

V

V

Q

Q

R

R

V

C

L

L

G

A

A

G

K

K

D

S

E

L

N

T

H

H

A

I

R

K

V

A

G

G

S

C

L

I

F

L

C

C

G

G

F

Y

I

L

K

K

I

L

L

T

P

P

V

M

F

F

I

L

F

M

V

V

L

M

P

P

G

G

L

M

F

I

A

S

Y

R

I

I

L

L

Y

Y

K

P

S

D

G

E

T

V

M

A

D

C

L

V

S

V

S

P

L

E

Q

V

T

C

V

R

G

R

S

V

N

C

G

G

E

T

T

E

V

V

L

G

N

C

T

S

K

N

G

I

I

A

Y

Y

T

P

L

R

M

L

I

V

T

V

Q

K

L

L

L

M

P

P

K

N

G

G

L

L

V

R

G

G

I

L

L

M

V

L

A

A

A

V

L

M

L

L

S

A

G

A

L

L

M

M

S

S

Q

S

I

L

A

A

G

S

A

I

L

F

N

N

S

S

I

S

A

S

T

T

L

L

S

F

S

T

Y

M

D

D

L

I

Y

Y

K

T

R

R

F

L

K

R

P

P

E

R

T

A

S

G

D

D

K

R

K

E

L

L

V

L

S

L

V

V

G

G

R

R

W

L

S

W

A

V

G

V

I

F

A

I

L

V

T

V

V

V

S

S

I

V

G

A

L

W

L

L

P

P

L

V

N

Q

S

A

Y

A

E

Q

-

G

S

Q

L

L

F
|
F

N

D

G

Y

I

I

N

Q

D

A

V

V

I

S

A

S

H

Y

I

L

A

A

P

P

I

V

T

S

C

A

V

V

F

F

L

V

L

L

G

A

V

L

F

F

W

V

K

P

K

R

A

V

S

N

A

E

K

Q

G

G

A

A

V95 (≠ N79) mutation to A: Strong reduction in D-glucose transporter activity. Confers partial resistance to empagliflozin inhibition.
F98 (= F82) mutation to A: Slightly decreases D-glucose transporter activity. Abolishes the binding to inhibitor, empagliflozin.
V157 (≠ F140) mutation to A: Decreases D-glucose transporter activity.
L283 (≠ Y246) mutation to M: Strong reduction in D-glucose transporter activity. Confers partial resistance to empagliflozin inhibition.
F453 (= F414) mutation to A: Slightly decreases D-glucose transporter activity. Greatly reduces the binding to inhibitor, empagliflozin.

8hg7A Structure of human sglt2-map17 complex with sotagliflozin (see paper)
33% identity, 81% coverage: 3:446/550 of query aligns to 2:465/590 of 8hg7A

query
sites
8hg7A

S

N

T

P

T

A

D

D

I

I

V

L

I

V

T

I

I

A

A

A

Y

Y

I

F

L

L

F

L

I

V

V

I

T

G

I

V

G

G

L

L

W

W

T

S

G

M

T

C

R

R

K

T

K

N

K

R

N

G

E

-

T

-

T

T

S

V

G

G

E

G

Y

Y

F

F

L

L

A

A

G

G

K

R

T

S

L

M

K

V

W

W

P

W

M

P

I

V

G

G

L

A

A

S

L

L

F

F

A
|
A

T
x
S

N
|
N

I
|
I

S
x
G

C

S

L
x
G

H
|
H

L

F

V

V

S
x
G

L
|
L

A

A

Q

G

S

T

G

G

F

A

D

A

S

S

G

G

L

L

L

A

N

V

G

A

N

G

F

F

E
|
E

W

W

M

N

A

A

A

L

F

F

T

V

L

V

I

L

L

L

A

G

L

W

L

L

F

F

I

A

P

P

F

V

Y

Y

I

L

R

T

S

A

G

G

I

V

S

I

T

T

L

M

P

P

D

Q

F

Y

L

L

E

R

R

K

R

R

Y

F

-

G

N

G

R

R

A

R

C

I

R

R

D

L

W

Y

L

L

A

S

F

V

I

L

S

S

I

L

L

F

S

L

A

Y

I

I

I

F

I

T

H

K

I

I

A

S

F

V

S

D

F

M

L

F

A

S

G

G

G

A

I

V

V

F

L

I

E

Q

T

Q

L

A

F

L

G

G

I

W

D

N

M

I

Y

Y

V

A

S

S

I

V

V

I

V

A

I

L

A

L

L

G

L

I

T

T

G

M

L

I

Y

Y

T

T

I

V

I

T

G

G

L

L

R

A

A

A

V

L

V

M

V

Y

T

T

E

D

T

T

I

V

Q

Q

S

T

L

F

V

V

L

I

I

L

T

G

G

G

A

A

I

C

I

I

I

L

T

M

Y

G

F

Y

A

A

W

F

N

H

K

E

V

V

G

G

W

Y

D

S

H

G

M

L

-

F

-

D

-

K

-

Y

-

L

-

G

-

A

-

T

T

S

A

L

I

T

L

V

Q

S

K

E

E

D

N

P

A

A

M

V

D

G

K

N

L

I

S

S

M

S

I

F

-

C

-

Y

R

R

P

P

I

R

G

P

D

D

K

S

-

Y

-

H

-

L

-

R

-

H

-

P

-

V

-

T

S

G

G

D

M

L

S

P

W

W

I

P

A

A

V

L

F

L

L

L

G

G

Y

L

P

T

V

I

L
x
V

G
x
S

I

G

W

W

Y
|
Y

W
|
W

C

C

A

S

D

D

Q

Q

T

V

I

I

V

V

Q

Q

R

R

V

C

L

L

G

A

A

G

K

K

D

S

E

L

N

T

H

H

A

I

R

K

V

A

G

G

S

C

L

I

F

L

C

C

G

G

F

Y

I

L

K
|
K

I

L

L

T

P

P

V

M

F

F

I

L

F

M

V

V

L

M

P

P

G

G

L

M

F

I

A

S

Y

R

I

I

L

L

Y

Y

K

P

S

D

G

E

T

V

M

A

D

C

L

V

S

V

S

P

L

E

Q

V

T

C

V

R

G

R

S

V

N

C

G

G

E

T

T

E

V

V

L

G

N

C

T

S

K

N

G

I

I

A

Y

Y

T

P

L

R

M

L

I

V

T

V

Q

K

L

L

L

M

P

P

K

N

G

G

L

L

V

R

G

G

I

L

L

M

V

L

A

A

A

V

L

M

L

L

S

A

G
x
A

L

L

M

M

S
|
S

Q
x
S

I

L

A

A

G

S

A

I

L

F

N

N

S

S

I

S

A

S

T

T

L

L

S

F

S

T

Y

M

D

D

L

I

Y

Y

K

T

R

R

F

L

K

R

P

P

E

R

T

A

S

G

D

D

K

R

K

E

L

L

V

L

S

L

V

V

G

G

R

R

W

L

S

W

A

V

G

V

I

F

A

I

L

V

T

V

V

V

S

S

I

V

G

A

L

W

L

L

P

P

L

V

N

Q

S

A

Y

A

E

Q

-

G

S

Q

L

L

F
|
F

N

D

G

Y

I

I

N
x
Q

D

A

V

V

I

S

A

S

H

Y

I

L

A

A

P

P

I

V

T

S

C

A

V

V

F

F

L

V

L

L

G

A

V

L

F

F

W

V

K

P

K

R

A

V

S

N

A

E

K

Q

G

G

A

A

binding (2S,3R,4R,5S,6R)-2-[4-chloranyl-3-[(4-ethoxyphenyl)methyl]phenyl]-6-methylsulfanyl-oxane-3,4,5-triol: N55 (= N59), G59 (≠ L63), H60 (= H64), G63 (≠ S67), L64 (= L68), E79 (= E83), V266 (≠ L249), S267 (≠ G250), Y270 (= Y253), W271 (= W254), K301 (= K284), F433 (= F414), Q437 (≠ N418)
binding sodium ion: A53 (= A57), S54 (≠ T58), I56 (= I60), G57 (≠ S61), A369 (≠ G352), S372 (= S355), S373 (≠ Q356)

Sites not aligning to the query:

binding : 579, 583, 584, 587, 588

8hinA Structure of human sglt2-map17 complex with phlorizin (see paper)
31% identity, 81% coverage: 3:446/550 of query aligns to 9:461/588 of 8hinA

query
sites
8hinA

S

N

T

P

T

A

D

D

I

I

V

L

I

V

T

I

I

A

A

A

Y

Y

I

F

L

L

F

L

I

V

V

I

T

G

I

V

G

G

L

L

W

W

T

S

G

M

T

C

R

R

K

T

K

-

N

-

E

-

T

-

S

-

G

-

E

-

Y

-

F

-

L

-

A

-

G

G

K

R

T

S

L

M

K

V

W

W

P

W

M

P

I

V

G

G

L

A

A
x
S

L

L

F

F

A
|
A

T
x
S

N

N

I

I

S
x
G

C

S

L

G

H

H

L

F

V

V

S

G

L

L

A

A

Q

G

S

T

G

G

F

A

D

A

S

S

G

G

L

L

L

A

N

V

G

A

N

G

F

F

E

E

W

W

M

N

A

A

A

L

F

F

T

V

L

V

I

L

L

L

A

G

L

W

L

L

F

F

I

A

P

P

F

V

Y

Y

I

L

R

T

S

A

G

G

I

V

S

I

T

T

L

M

P

P

D

Q

F

Y

L

L

E

R

R

K

R

R

Y

F

N

G

-

G

R

R

A

R

C

I

R

R

D

L

W

Y

L

L

A

S

F

V

I

L

S

S

I

L

L

F

S

L

A

Y

I

I

I

F

I

T

H

K

I

I

A

S

F

V

S

D

F

M

L

F

A

S

G

G

G

A

I

V

V

F

L

I

E

Q

T

Q

L

A

F

L

G

G

I

W

D

N

M

I

Y

Y

V

A

S

S

I

V

V

I

V

A

I

L

A

L

L

G

L

I

T

T

G

M

L

I

Y

Y

T

T

I

V

I

T

G

G

L

L

R

A

A

A

V

L

V

M

V

Y

T

T

E
x
D

T

T

I

V

Q

Q

S
x
T

L

F

V

V

L

I

I

L

T

G

G

G

A

A

I

C

I

I

I

L

T

M

Y

G

F

Y

A

A

W

F

N

H

K

E

V

V

G

G

W

Y

D

S

H

G

M

L

-

F

-

D

-

K

-

Y

-

L

-

G

-

A

-

T

T

S

A

L

I

T

L

V

Q

S

K

E

E

D

N

P

A

A

M

V

D

G

K

N

L

I

S

S

M

S

I

F

-

C

-

Y

R

R

P

P

I

R

G

P

D

D

K

S

-

Y

-

H

-

L

-

R

-

H

-

P

-

V

-

T

S

G

G

D

M

L

S

P

W

W

I

P

A

A

V

L

F

L

L

L

G

G

Y

L

P

T

V

I

L

V

G

S

I

G

W

W

Y

Y

W

W

C

C

A

S

D

D

Q

Q

T

V

I

I

V

V

Q

Q

R
|
R

V

C

L

L

G

A

A

G

K

K

D

S

E

L

N

T

H

H

A

I

R

K

V

A

G

G

S

C

L

I

F

L

C

C

G

G

F

Y

I

L

K

K

I

L

L

T

P

P

V

M

F

F

I

L

F

M

V

V

L

M

P

P

G

G

L

M

F

I

A

S

Y

R

I

I

L

L

Y

Y

K

P

S

D

G

E

T

V

M

A

D

C

L

V

S

V

S

P

L

E

Q

V

T

C

V

R

G

R

S

V

N

C

G

G

E

T

T

E

V

V

L

G

N

C

T

S

K

N

G

I

I

A

Y

Y

T

P

L

R

M

L

I

V

T

V

Q

K

L

L

L

M

P

P

K

N

G

G

L

L

V

R

G

G

I

L

L

M

V

L

A

A

A

V

L

M

L

L

S

A

G

A

L

L

M

M

S

S

Q
x
S

I

L

A

A

G

S

A

I

L

F

N

N

S

S

I

S

A

S

T

T

L

L

S

F

S

T

Y

M

D

D

L

I

Y

Y

K

T

R

R

F

L

K

R

P

P

E

R

T

A

S

G

D

D

K

R

K

E

L

L

V

L

S

L

V

V

G

G

R

R

W

L

S

W

A

V

G

V

I

F

A

I

L

V

T

V

V

V

S

S

I

V

G

A

L

W

L

L

P

P

L

V

N

Q

S

A

Y

A

E

Q

-

G

S

Q

L

L

F

F

N

D

G

Y

I

I

N

Q

D

A

V

V

I

S

A

S

H

Y

I

L

A

A

P

P

I

V

T

S

C

A

V

V

F

F

L

V

L

L

G

A

V

L

F

F

W

V

K

P

K

R

A

V

S

N

A

E

K

Q

G

G

A

A

binding 1-[2-[(2S,3R,4S,5S,6R)-6-(hydroxymethyl)-3,4,5-tris(oxidanyl)oxan-2-yl]oxy-4,6-bis(oxidanyl)phenyl]-3-(4-hydroxyphenyl)propan-1-one: S46 (≠ A54), A49 (= A57), S50 (≠ T58), G53 (≠ S61), D177 (≠ E184), T181 (≠ S188), R276 (= R263), S369 (≠ Q356)

Sites not aligning to the query:

binding : 574, 578, 581, 582, 585, 586

7yniA Structure of human sglt1-map17 complex bound with substrate 4d4fdg in the occluded conformation (see paper)
32% identity, 90% coverage: 3:497/550 of query aligns to 7:499/566 of 7yniA

query
sites
7yniA

S

N

T

A

D

D

I

I

V

S

I

I

T

I

I

V

A

I

Y

Y

I

F

L

V

F

V

I

V

V

M

T

A

I

V

G

G

L

L

W

W

T

A

G

M

T

F

R

R

K

-

N

-

E

-

T

-

S

-

G

-

E

-

Y

-

F

-

L

-

A

-

G

-

K

-

T

S

L

M

K

V

W

W

P

W

M

P

I

I

G

G

L

A

A

S

L

L

F

F

A

A

T

S

N

N

I

I

S

G

C

S

L

G

H
|
H

L

F

V

V

S

G

L

L

A

A

Q

G

S

T

G

G

F

A

D

A

S

S

G

G

L

I

L

A

N

I

G

G

N

G

F

F

E
|
E

W

W

M

N

A

A

A

L

F

V

T

L

L

V

I

V

L

V

L

L

A

G

L

W

L

L

F

F

I

V

P

P

F

I

Y

Y

I

I

R

K

S

A

G

G

I

V

S

V

T

T

L

M

P

P

D

E

F

Y

L

L

E

R

R

K

R

R

Y

F

N

G

-

G

R

Q

A

R

C

I

R

Q

D

V

W

Y

L

L

A

S

F

L

I

L

S

S

I

L

L

L

S

L

A

Y

I

I

I

F

I

T

H

K

I

I

A

S

F

A

S

D

F

I

L

F

A

S

G

G

G

A

I

I

V

F

L

I

E

N

T

L

L

A

F

L

G

G

I

L

D

N

M

L

Y

Y

V

L

S

A

I

I

V

F

V

L

I

L

A

L

L

A

L

I

T

T

G

A

L

L

Y

Y

T

T

I

I

I

-

G

-

L

-

R

-

A

-

V

V

V

I

V

Y

T

T

E

D

T

T

I

L

Q

Q

S

T

L

V

V

I

L

M

I

L

T

V

G

G

A

S

I

L

I

I

I

L

T

T

Y

G

F

F

A

A

W

F

N

H

K

E

V

V

G

G

W

Y

D

D

-

A

-

F

-

M

-

E

-

K

H

Y

M

M

T

K

A

A

I

I

-

P

-

T

-

I

L

V

Q

S

K

D

E

G

N

N

A

T

M

T

D

F

K

Q

L

E

S

K

M

C

I

Y

R

T

P

P

I

R

G

A

D

D

K

S

-

F

-

H

-

I

-

F

-

R

-

D

-

P

-

L

-

T

S

G

G

D

M

L

S

P

W

W

I

P

A

G

V

F

F

I

L

F

G

G

Y

M

P

S

V

I

L
|
L

G

T

I

L

W

W

Y
|
Y

W

W

C

C

A

T

D

D

Q

Q

T

V

I

I

V

V

Q

Q

R

R

V

C

L

L

G

S

A

A

K

K

D

N

E

M

N

S

H

H

A

V

R

K

V

G

G

G

S

C

L

I

F

L

C

C

G

G

F

Y

I

L

K

K

I

L

L

M

P

P

V

M

F

F

I

I

F

M

V

V

L

M

P

P

G

G

L

M

F

I

A

S

Y

R

I

I

L

L

Y

Y

K

T

S

E

G

K

T

I

M

A

D

C

L

V

S

V

S

P

L

S

Q

E

T

C

V

E

G

K

S

Y

N

C

G

G

E

T

T

K

V

V

L

G

N

C

T

T

K

N

G

I

I

A

Y

Y

T

P

L

T

M

L

I

V

T

V

Q

E

L

L

L

M

P

P

K

N

G

G

L

L

V

R

G

G

I

L

L

M

V

L

A

S

A

V

L

M

L

L

S

A

G

S

L

L

M

M

S

S

Q

S

I

L

A

T

G

S

A

I

L

F

N

N

S

S

I

A

A

S

T

T

L

L

S

F

S

T

Y

M

D

D

L

I

Y

Y

K

A

R

K

F

V

K

R

P

K

E

R

T

A

S

S

D

E

K

K

K

E

L

L

V

M

S

I

V

A

G

G

R

R

W

L

S

F

A

I

G

L

I

V

A

L

L

I

T

G

V

I

S

S

I

I

G

A

L

W

L

V

P

P

L

I

L

V

N

Q

S

S

Y

A

E

Q

S

S

-

G

-

Q

L

L

F
|
F

N

D

G

Y

I

I

N
x
Q

D

S

V

I

I

T

A

S

H

Y

I

L

A

G

P

P

I

I

T

A

C

A

V

V

F

F

L

L

G

A

V

I

F

F

W

W

K

K

K

R

A

V

S

N

A

E

K

P

G

G

A

A

Q

F

Y

W

T

G

L

L

L

I

L

L

G

G

S

L

I

L

I

I

G

G

A

I

G

S

V

R

F

M

V

I

V

T

N

E

K

F

V

A

Y

Y

G

G

T

N

-

C

E

P

T

T

I

I

G

C

Q

G

I

V

P

H

F

Y

M

L

M

Y

M

F

A

A

F

I

Y

I

L

L

F

F

C

A

I

I

C

S

V

F

L

I

I

T

Q

I

V

V

F

I

S

S

binding (2R,3R,4R,5S,6R)-5-fluoranyl-6-(hydroxymethyl)oxane-2,3,4-triol: H51 (= H64), E70 (= E83), L248 (= L249), Y252 (= Y253), F415 (= F414), Q419 (≠ N418)

Sites not aligning to the query:

binding : 548, 552, 555, 556, 559, 560, 563, 564

Q9ET37 Solute carrier family 5 member 4A; SGLT3-a from Mus musculus (Mouse) (see paper)
29% identity, 89% coverage: 3:492/550 of query aligns to 25:540/656 of Q9ET37

query
sites
Q9ET37

S

N

T

A

D

D

I

I

V

S

I

V

T

I

I

V

A

I

Y

Y

I

F

L

V

F

V

I

V

V

M

T

A

I

V

G

G

L

V

W

W

T

A

G

-

T

-

R

-

K

M

K

L

K

K

N

T

E

N

E

R

T

S

T

T

S

V

G

G

E

G

Y

F

F

F

L

L

A

A

G

G

K

R

T

S

L

M

K

T

W

W

P

W

M

P

I

M

G

G

L

A

A

S

L

L

F

F

A

A

T

S

N

N

I

I

S

G

C

S

L

G

H

H

L

F

V

V

S

G

L

L

A

A

Q

G

S

T

G

G

F

A

D

A

S

S

G

G

L

I

L

A

N

V

G

T

N

A

F

F

E

E

W

S

M

H

A

S

A

F

F

A

T

L

L

L

I

L

L

V

L

L

A

G

L

W

L

I

F

F

I

V

P

P

F

I

Y

Y

I

I

R

K

S

A

G

G

I

V

S

M

T

T

L

M

P

P

D

E

F

Y

L

L

E

K

R

K

R

R

Y

F

N

G

-

G

R

K

A

R

C

L

R

Q

D

I

W

Y

L

L

A

S

F

I

I

L

S

F

I

L

L

F

S

I

A

C

I

V

I

I

I

L

H

T

I

I

A

S

F

A

S

D

F

I

L

F

A

S

G

G

G

A

I

I

V

F

L

I

E

K

T

L

L

A

F

L

G

G

I

L

D

N

M

L

Y

Y

V

L

S

A

I

I

V

L

V

I

I

L

A

L

L

A

L

I

T

T

G

A

L

I

Y

F

T

T

I

I

I

T

G

G

L

L

R

A

A

S

V

V

V

I

V

Y

T

T

E

D

T

T

I

V

Q

Q

S

A

L

V

V

I

L

M

I

L

T

V

G

G

A

S

I

F

I

I

I

L

T

M

Y

V

F

F

A

A

W

F

N

V

K

E

V

V

G

G

W

Y

D

E

H

S

M

F

T

T

-

E

-

K

-

F

-

M

-

N

-

A

-

I

-

P

A

S

I

V

L

V

Q

E

K

G

E

D

N

N

A

L

M

T

D

I

K

N

L

S

S

R

M

C

I

Y

R

T

P

P

I

Q

G

P

D

D

K

S

-

F

-

H

-

I

-

F

-

R

-

D

-

P

-

V

-

T

S

G

G

D

M

I

S

P

W

W

I

P

A

G

V

T

F

A

L

F

G

G

Y

M

P

P

V

I

L

T

G

A

I

L

W

W

Y

Y

W

W

C

C

A

I

D

N

Q

Q

T

V

I

I

V

V

Q

Q

R

R

V

C

L

L

G

C

A

G

K

K

D

N

E

L

N

S

H

H

A

V

R

K

V

A

G

A

S

C

L

I

F

L

C

C

G

G

F

Y

I

L

K

K

I

L

L

L

P

P

V

L

F

F

I

F

F

M

V

V

L

M

P

P

G

G

L

M

F

I

A

S

Y

R

I

I

L

L

Y

Y

K

T

S

D

G

M

T

V

M

A

D

C

L

V

S

V

S

P

L

S

Q

E

T

C

V

V

G

K

S

H

N

C

G

G

E

V

T

D

V

V

L

G

N

C

T

T

K

N

G

Y

I

A

Y

Y

T

P

L

M

M

L

I

V

T

L

Q

K

L

L

L

M

P

P

K

M

G

G

L

L

V

R

G

G

I

L

L

M

V

L

A

S

A

V

L

M

L

L

S

A

G

S

L

L

M

M

S

S

Q

S

I

L

A

T

G

S

A

V

L

F

N

N

S

S

I

A

A

S

T

T

L

L

S

F

S

T

Y

I

D

D

L

L

Y

Y

K

T

R

K

F

I

K

R

P

K

E

K

T

A

S

S

D

E

K

R

K

E

L

L

V

L

S

I

V

A

G

G

R

R

W

L

S

F

A

V

G

S

I

V

A

L

L

I

T

V

V

T

S

S

I

I

G

L

L

W

L

V

P

P

L

I

L

V

N

E

S

V

Y

S

E

Q

-

G

S

Q

L

L

F

V

N

H

G

Y

I

T

N
x
E

D

A

V

I

I

S

A

S

H

Y

I

L

A

G

P

P

I

I

T

A

C

A

V

V

F

F

L

L

L

V

G

A

V

V

F

F

W

C

K

K

K

R

A

A

S

N

A

E

K

Q

G

G

A

A

Q

F

Y

W

T

G

L

L

L

M

L

V

G

G

S

L

I

V

I

M

G

G

A

L

G

I

V

R

F

M

V

I

V

A

N

E

K

F

V

S

Y

Y

G

G

T

T

-

G

-

S

-

C

-

L

-

A

-

P

-

S

-

C

E

P

T

K

I

I

G

C

Q

G

I

V

P

H

F

Y

M

L

M

Y

M

F

A

A

F

I

Y

I

L

L

F

F

C

F

I

V

C

C

V

I

L

L

I

V

E457 (≠ N418) mutation to Q: Confers sodium-dependent sugar transport activity not found in the wild type protein.

7ynjA Structure of human sglt2-map17 complex bound with substrate amg in the occluded conformation (see paper)
31% identity, 80% coverage: 7:446/550 of query aligns to 1:443/564 of 7ynjA

query
sites
7ynjA

I

I

V

L

I

V

T

I

I

A

A

A

Y

Y

I

F

L

L

F

L

I

V

V

I

T

G

I

V

G

G

L

L

W

W

T

S

G

M

T

C

R

R

K

R

K

-

N

-

E

-

T

-

S

-

G

-

E

-

Y

-

F

-

L

-

A

-

G

-

K

-

T

S

L

M

K

V

W

W

P

W

M

P

I

V

G

G

L

A

A

S

L

L

F

F

A

A

T

S

N

N

I

I

S

G

C

S

L

G

H
|
H

L

F

V

V

S

G

L

L

A

A

Q

G

S

T

G

G

F

A

D

A

S

S

G

G

L

L

L

A

N

V

G

A

N

G

F

F

E
|
E

W

W

M

N

A

A

A

L

F

F

T

V

L

V

I

L

L

L

A

G

L

W

L

L

F

F

I

A

P

P

F

V

Y

Y

I

L

R

T

S

A

G

G

I

V

S

I

T

T

L

M

P

P

D

Q

F

Y

L

L

E

R

R

K

R

R

Y

F

N

G

-

G

R

R

A

R

C

I

R

R

D

L

W

Y

L

L

A

S

F

V

I

L

S

S

I

L

L

F

S

L

A

Y

I

I

I

F

I

T

H

K

I

I

A

S

F

V

S

D

F

M

L

F

A

S

G

G

G

A

I

V

V

F

L

I

E

Q

T

Q

L

A

F

L

G

G

I

W

D

N

M

I

Y

Y

V

A

S

S

I

V

V

I

V

A

I

L

A

L

L

G

L

I

T

T

G

M

L

I

Y

Y

T

T

I

V

I

T

G

G

L

L

R

-

A

-

V

-

V

Y

T

T

E

D

T

T

I

V

Q

Q

S

T

L

F

V

V

L

I

I

L

T

G

G

G

A

A

I

C

I

I

I

L

T

M

Y

G

F

Y

A

A

W

F

N

H

K

E

V

V

G

G

W

Y

D

S

H

G

M

L

-

F

-

D

-

K

-

Y

-

L

-

G

-

A

-

T

T

S

A

L

I

T

L

V

Q

S

K

E

E

D

N

P

A

A

M

V

D

G

K

N

L

I

S

S

M

S

I

F

-

C

-

Y

R

R

P

P

I

R

G

P

D

D

K

S

-

Y

-

H

-

L

-

R

-

H

-

P

-

V

-

T

S

G

G

D

M

L

S

P

W

W

I

P

A

A

V

L

F

L

L

L

G

G

Y

L

P

T

V

I

L

V

G

S

I

G

W

W

Y
|
Y

W

W

C

C

A

S

D

D

Q

Q

T

V

I

I

V

V

Q

Q

R

R

V

C

L

L

G

A

A

G

K

K

D

S

E

L

N

T

H

H

A

I

R

K

V

A

G

G

S

C

L

I

F

L

C

C

G

G

F

Y

I

L

K

K

I

L

L

T

P

P

V

M

F

F

I

L

F

M

V

V

L

M

P

P

G

G

L

M

F

I

A

S

Y

R

I

I

L

L

Y

Y

K

P

S

D

G

E

T

V

M

A

D

C

L

V

S

V

S

P

L

E

Q

V

T

C

V

R

G

R

S

V

N

C

G

G

E

T

T

E

V

V

L

G

N

C

T

S

K

N

G

I

I

A

Y

Y

T

P

L

R

M

L

I

V

T

V

Q

K

L

L

L

M

P

P

K

N

G

G

L

L

V

R

G

G

I

L

L

M

V

L

A

A

A

V

L

M

L

L

S

A

G

A

L

L

M

M

S

S

Q

S

I

L

A

A

G

S

A

I

L

F

N

N

S

S

I

S

A

S

T

T

L

L

S

F

S

T

Y

M

D

D

L

I

Y

Y

K

T

R

R

F

L

K

R

P

P

E

R

T

A

S

G

D

D

K

R

K

E

L

L

V

L

S

L

V

V

G

G

R

R

W

L

S

W

A

V

G

V

I

F

A

I

L

V

T

V

V

V

S

S

I

V

G

A

L

W

L

L

P

P

L

V

N

Q

S

A

Y

A

E

Q

-

G

S

Q

L

L

F
|
F

N

D

G

Y

I

I

N
x
Q

D

A

V

V

I
x
S

A

S

H

Y

I

L

A

A

P

P

I

V

T

S

C

A

V

V

F

F

L

V

L

L

G

A

V

L

F

F

W

V

K

P

K

R

A

V

S

N

A

E

K

Q

G

G

A

A

binding methyl alpha-D-glucopyranoside: H42 (= H64), E61 (= E83), Y248 (= Y253), F411 (= F414), Q415 (≠ N418), S418 (≠ I421)

Sites not aligning to the query:

binding : 550, 553, 557, 558, 561, 562, 564

3dh4A Crystal structure of sodium/sugar symporter with bound galactose from vibrio parahaemolyticus (see paper)
36% identity, 73% coverage: 43:446/550 of query aligns to 19:421/512 of 3dh4A

query
sites
3dh4A

G

G

K

K

T

S

L

L

K

P

W

W

P

W

M

A

I

V

G

G

L

A

A

S

L

L

F

I

A

A

T

A

N

N

I
|
I

S

S

C

A

L

E

H
x
Q

L

F

V

I

S

G

L

M

A

S

Q

G

S

S

G

G

F

Y

D

S

S

I

G

G

L

L

L

A

N

I

G

A

N

S

F
x
Y

E
|
E

W

W

M

M

A
x
S

A

A

F

I

T

T

L

L

I

I

L

I

L

V

A

G

L

K

L

Y

F

F

I

L

P

P

F

I

Y

F

I

I

R

E

S

K

G

G

I

I

S

Y

T

T

L

I

P

P

D

E

F

F

L

V

E

E

R

K

R

R

Y

F

N

N

R

K

A

K

C

L

R

K

D

T

W

I

L

L

A

A

F

V

I

F

S

W

I

I

L

S

S

L

A

Y

I

I

I

F

I

V

H

N

I

L

A

T

F

S

S

V

F

L

L

Y

A

L

G

G

I

L

V

A

L

L

E

E

T

T

L

I

F

L

G

G

I

I

D

P

M

L

Y

M

V

Y

S

S

I

I

V

L

V

G

I

L

A

A

L

L

L

F

T

A

G

L

L

V

Y

Y

T

S

I

I

I

-

G

-

L

-

R

-

A

-

V

V

V

W

T

T

E

D

T

V

I

I

Q

Q

S

V

L

F

V

F

L

L

I

V

T

L

G

G

A

G

I

F

I

M

I

T

T

T

Y

Y

F

M

A

A

W

V

N

S

K

F

V

I

G

G

W

T

D

D

H

G

M

W

T

F

A

A

I

G

L

V

Q

S

K

K

-

M

-

V

E

D

N

A

A

A

M

P

D

G

K

H

L

F

S

E

M

M

I

I

R

L

P

D

I

Q

G

S

D

N

K

P

S

Q

G

Y

M

M

S

N

W

L

-

P

-

G

I

I

A

A

V

V

F

L

L

I

G

G

Y

-

P

-

V

-

L

-

G

G

I

L

W

W

-

V

-

A

-
x
N

-

L

-

Y

Y

Y

W
|
W

C

G

A

F

D

N

Q

Q

T

Y

I

I

V

I

Q

Q

R

R

V

T

L

L

G

A

A

A

K

K

D

S

E

V

N

S

H

E

A

A

R

Q

V

K

G

G

S

I

L

V

F

F

C

A

G

A

F

F

I

L

K
|
K

I

L

L

I

P

V

V

P

F

F

I

L

F

V

V

V

L

L

P

P

G

G

L

I

F

A

A

A

Y

Y

I

V

L

I

Y

T

K

S

S

D

G

P

T

Q

M

L

D

M

L

A

S

S

S

L

L

G

Q

D

T

I

V

A

G

A

S

T

N

N

G

L

E

P

T

S

V

A

L

A

N

N

T

A

K

D

G

K

I

A

Y

Y

T

P

L

W

M

L

I

-

T

T

Q

Q

L

F

L

L

P

P

K

V

G

G

L

V

V

K

G

G

I

V

L

V

V

F

A

A

L

L

L

A

S

A

G
x
A

L

I

M

V

S
|
S

Q

S

I

L

A

A

G

S

A

M

L

L

N

N

S

S

I

T

A

A

T

T

L

I

S

F

S

T

Y

M

D

D

L

I

Y

Y

K

K

R

E

F

Y

-

I

K

S

P

P

E

D

T

S

S

G

D

D

K

H

K

K

L

L

V

V

S

N

V

V

G

G

R

R

W

T

S

A

A

A

G

V

I

V

A

A

L

L

T

I

V

I

S

A

I

C

G

L

L

I

L

A

P

P

L

M

L

L

N

G

S

G

Y

I

E

G

S

Q

L

A

F

F

N

Q

G

Y

I

I

N
x
Q

D

E

V

Y

I

T

A

G

H

L

I

V

A

S

P

P

P

G

I

I

T

L

C

A

V

V

F

F

L

L

G

G

V

L

F

F

W

W

K

K

A

T

S

T

A

S

K

K

G

G

A

A

binding beta-D-galactopyranose: Q40 (≠ H64), Y58 (≠ F82), E59 (= E83), S62 (≠ A86), N225 (vs. gap), W229 (= W254), K259 (= K284), Q393 (≠ N418)
binding sodium ion: I36 (= I60), A326 (≠ G352), S329 (= S355)

Q9Y289 Sodium-dependent multivitamin transporter; Na(+)-dependent multivitamin transporter; hSMVT; Solute carrier family 5 member 6 from Homo sapiens (Human) (see 10 papers)
24% identity, 79% coverage: 20:456/550 of query aligns to 42:466/635 of Q9Y289

query
sites
Q9Y289

I

I

G

G

L

L

W

Y

T

H

G

A

T

C

R

R

K

G

K

W

K

G

N

R

E

H

E

-

T

-

T

T

S

V

G

G

E

E

Y

L

F

L

L

M

A

A

G

D

K

R

T

K

L

M

K

G

W
x
C

P

L

M

P

I

V

G

A

L

L

A

S

L

L

F

L

A

A

T
|
T

N

F

I

Q

S

S

C

A

L

V

H

A

L

I

V

L

S

G

L

V

A

P

Q

S

S

E

G

I

F

Y

D
x
R

S
x
F

G
|
G

L
x
T

L
x
Q

N
x
Y

G
x
W

N
x
F

F
x
L

E
x
G

W
x
C

M
x
C

A
x
Y

A
x
F

F
x
L

T
x
G

L
|
L

I
x
L

L
x
I

L
x
P

A
|
A

L
x
H

L
x
I

F
|
F

I
|
I

P
|
P

F
x
V

Y
x
F

I
x
Y

R
|
R

S
x
L

G
x
H

I
x
L

S
x
T

T
x
S

L
x
A

P
x
Y

D
x
E

F
x
Y

L
|
L

E
|
E

R
x
L

R
|
R

Y
x
F

N
|
N

R
x
K

A
x
T

C
x
V

R
|
R

D
x
V

W

-

L
x
C

A
x
G

F
x
T

I
x
V

S
x
T

I
x
F

L
x
I

S
x
F

A
x
Q

I
x
M

I
x
V

I
|
I

H
x
Y

I
x
M

A
x
G

F
x
V

S
x
V

F
x
L

L
x
Y

A
|
A

G
x
P

G
x
S

I
x
L

V
x
A

L
|
L

E
x
N

T
x
A

L
x
V

F
x
T

G
|
G

I
x
F

D
|
D

M
x
L

Y
x
W

V
x
L

S
|
S

I
x
V

V
x
L

V
x
A

I
x
L

A
x
G

L
x
I

L
x
V

T
x
C

G
x
T

L
x
V

Y
|
Y

T
|
T

I
x
A

I
x
L

G
|
G

L
|
L

R
x
K

A
|
A

V
|
V

V
x
I

V
x
W

T
|
T

E
x
D

T
x
V

I
x
F

Q
|
Q

S
x
T

L
|
L

V
|
V

L
x
M

I
x
F

T
x
L

G
|
G

A
x
Q

I
x
L

I
x
A

I
x
V

T
x
I

Y
x
I

F
x
V

A
x
G

W
x
S

N
x
A

K
|
K

V
|
V

G
|
G

W
x
L

D
x
G

H
x
R

M
x
V

T
x
W

A
|
A

I
x
V

L
x
A

Q
x
S

K
x
Q

E
x
H

N
x
G

A
x
R

M
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I

D
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F

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L

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-

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D

P
|
P

I
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D

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P

D
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F

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R

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F

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W

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T

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A

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F

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x
G

G
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G

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M

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-

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F

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A

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x
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L

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F

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S

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G

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S

Q
x
T

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I

A
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S

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S

A
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A

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F

N
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N

S
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S

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x
L

A
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A

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T

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V

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T

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M

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D

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L

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W

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F

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P

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E

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x
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S

D
x
E

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A

K
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x
A

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x
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R
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R

W

-

S

-

A

-

G

-

I

-

A
x
G

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L

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x
A

V
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F

S
x
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x
Y

G
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G

L
|
L

P
x
C

L
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L

L
x
G

N
x
M

S
x
A

Y
|
Y

E
x
I

S
|
S

-
x
S

-
x
Q

-
x
M

-
x
G

-
x
P

L
x
V

F
x
L

N
x
Q

G
x
A

I
x
A

N
x
I

D
x
S

V
x
I

I
x
F

A
x
G

H
x
M

I
x
V

A
x
G

P
x
G

P
|
P

I
x
L

T
x
L

C
x
G

V
x
L

F
|
F

L
x
C

L
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L

G
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G

V
x
M

F
|
F

W
x
F

K
x
P

K
x
C

A
|
A

S
x
N

A
x
P

K
x
P

G
|
G

A
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A

Q
x
V

Y
x
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G

L
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L

L
x
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G

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L

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V

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M

C68 (≠ W48) mutation to A: No effect on biotin transport.
T78 (= T58) mutation to A: Reduced membrane localization. Decrease in biotin transport.
C104 (≠ W84) mutation to A: No effect on biotin transport.
R123 (= R103) to L: in SMVTD; reduced membrane localization; impaired biotin transport
S128 (≠ T108) mutation to A: No effect on biotin transport.
N138 (= N118) mutation to A: Reduced protein levels. Decrease in biotin transport.
C144 (≠ L125) mutation to A: No effect on biotin transport.
Y162 (≠ L143) to C: in COMNB; no effect on membrane localization
C187 (≠ T168) mutation to A: No effect on biotin transport.
S242 (≠ D223) mutation to A: No effect on biotin transport.
S283 (≠ G266) mutation to A: No effect on protein levels or membrane localization.
T286 (≠ D269) mutation to A: Resistant to phorbol 12-myristate 13-acetate (PMA)-induced inhibition of biotin transport. No effect on protein levels or membrane localization.
C294 (≠ S277) mutation to A: Reduced membrane localization. Decrease in biotin transport (decreased Vmax, no change in Km).; mutation C->S,M: Decrease in biotin transport.
C309 (≠ V292) mutation to A: No effect on biotin transport.
C358 (≠ A348) mutation to A: No effect on biotin transport.
T366 (≠ Q356) mutation to A: No effect on biotin transport.
R400 (= R390) to T: in SMVTD; impaired biotin transport; dbSNP:rs370950187
C410 (≠ P405) mutation to A: No effect on biotin transport.
S429 (≠ D419) to G: in COMNB; no effect on membrane localization
C443 (≠ L433) mutation to A: No effect on biotin transport.
C450 (≠ K440) mutation to A: No effect on biotin transport.

Sites not aligning to the query:

94:635 natural variant: Missing (in SMVTD and COMNB; reduced membrane localization; impaired biotin transport; dbSNP:rs994218778)
481 S → F: in dbSNP:rs1395
489 N→A: Slight decrease in protein levels. Decrease in biotin transport.
498 N→A: No effect on biotin transport.
534 N→A: No effect on biotin transport.
567:635 mutation Missing: Loss of biotin transport. Loss of membrane localization.
570:635 mutation Missing: Loss of biotin transport. Loss of membrane localization.
575:635 mutation Missing: Partial loss (75%) of biotine transport. Apical membrane localization and intracellular structure localization in polarized cells.
577 C→A: No effect on biotin transport.
583 C→A: No effect on biotin transport.
584:635 mutation Missing: Partial loss (75%) of biotine transport. Apical membrane localization and intracellular structure localization in polarized cells.
600:635 mutation Missing: Partial loss (75%) of biotine transport. Apical membrane localization and intracellular structure localization in polarized cells.
612:635 mutation Missing: Partial loss (75%) of biotine transport. Apical membrane localization and intracellular structure localization in polarized cells.
616:635 mutation Missing: Loss of apical membrane localization in polarized cells. Basolateral localization in polarized cells.
620:635 mutation Missing: Partial loss (25%) of biotine transport. No change in apical membrane localization in polarized cells.
624:635 mutation Missing: Partial loss (25%) of biotine transport. No change in apical membrane localization in polarized cells.
627 T→A: No effect on biotin transport.
628 mutation C->A,S: Decrease in biotin transport.
632:635 mutation Missing: Partial loss (25%) of biotine transport. No change in apical membrane localization in polarized cells.

Q92911 Sodium/iodide cotransporter; Na(+)/I(-) cotransporter; Natrium iodide transporter; Sodium-iodide symporter; Na(+)/I(-) symporter; Solute carrier family 5 member 5 from Homo sapiens (Human) (see 3 papers)
24% identity, 79% coverage: 12:445/550 of query aligns to 21:444/643 of Q92911

query
sites
Q92911

A

A

Y

L

I

M

L

L

F

L

I

V

V

S

T

T

-

G

I

I

G

G

L

L

W

W

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T

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R

-

K

-

K

A

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N

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Q

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A

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L

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S

A

A

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F

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A

L

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H

Q

L

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V

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A

P

Q

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S

E

G

A

F

Y

D

R

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Y

G

G

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L

L

-

N

-

G

-

N

K

F

F

E

L

W

W

M

M

A

C

A

L

F

G

T

Q

L

L

-

L

-

N

-

S

I

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L

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A
|
A

L

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F

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P

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F

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F

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S

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x
H

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x
D

D

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x
T

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C

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D

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A

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P

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-

N

-

T

-

K

-

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P

I

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Y

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M

D

I

I

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F

Q

E

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D

L

L

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P

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-

G

G

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P

G

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F

V

L

A

A

A

C

L

A

L

Y

S

S

G

G

L

T

M

L

S

S

Q

T

I

A

A

S

G

T

A

S

L

I

N

N

S

A

I

M

A

A

T

A

L

V

S

T

S

V

Y

E

D

D

L

L

Y

I

K

K

R

P

F

R

K

L

P

R

E

S

T

L

S

A

D

P

K

R

K

K

L

L

V

V

S

I

V

I

G

S

R

K

W

G

S

L

A

S

-

L

-

I

-

Y

G

G

I

S

A

A

L

C

T

L

V

T

S

V

I

A

G

A

L

L

L

S

P

S

L

L

N

G

S

G

Y

-

E

-

S

G

L

V

F

L

N

Q

G

G

I

S

N

F

D

T

V

V

I

M

A

G

H

V

I

I

A

S

P

G

P

P

I

L

T

L

C

G

V

A

F

F

L

I

L

L

G

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F

F

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L

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P

K

A

A

C

S

N

A

T

K

P

G

G

A102 (= A94) natural variant: A -> P
H226 (≠ E219) mutation H->A,D,E,K: Significant loss of iodide transport activity but no effect on its localization to the cell membrane.
D237 (≠ G232) mutation to A: Loss of localization to the cell membrane, significant loss of iodide transport activity but no effect on homodimerization.
Y242 (≠ M237) Required for homodimerization; mutation to A: Loss of localization to the cell membrane, significant loss of iodide transport activity but no effect on homodimerization. Reduced homodimerization; when associated with A-471. Loss of iodide transport activity; when associated with F-535.
T243 (≠ S238) Required for homodimerization; mutation to A: Loss of localization to the cell membrane, significant loss of iodide transport activity but no effect on homodimerization. Reduced homodimerization; when associated with A-471.

Sites not aligning to the query:

471 Required for homodimerization; Q→A: No effect on localization to the cell membrane, iodide transport activity and homodimerization. Significant loss of homodimerization; when associated with A-242 or A243.
525 A→F: Loss of localization to the cell membrane, significant loss of iodide transport activity but no effect on homodimerization. Loss of iodide transport activity; when associated with A-242.
536 T → Q: requires 2 nucleotide substitutions
556 S → Q: requires 2 nucleotide substitutions

Q8N695 Sodium-coupled monocarboxylate transporter 1; Apical iodide transporter; Electrogenic sodium monocarboxylate cotransporter; Sodium iodide-related cotransporter; Solute carrier family 5 member 8 from Homo sapiens (Human) (see 3 papers)
22% identity, 79% coverage: 33:467/550 of query aligns to 39:463/610 of Q8N695

query
sites
Q8N695

E

Q

T

Q

T

T

S

S

G

K

E

D

Y

F

F

L

L

M

A

G

G

G

K

R

T

R

L

M

K

T

W

A

P

V

M

P

I

V

G

A

L

L

A

S

L

L

F

T

A

A

T

S

N

F

I

M

S

S

C

A

L

V

H

T

L

V

V

L

S

G

L

T

A

P

Q

S

S

E

G

V

F

Y

D

R

S

F

G

G

L

A

L

I

N

F

G

S

N

I

F

F

E

A

W

F

M

T

A

Y

A

F

F

F

T

V

L

V

I

V

L

I

L

S

A

A

L

E

L

V

F

F

I

L

P

P

F

V

Y

F

I

Y

R

K

S

L

G

G

I

I

S

T

T

S

L

T

P

Y

D

E

F

Y

L

L

E

E

R

L

R

R

Y

F

N

N

R

K

A

-

C

C

R

V

D

R

W

L

L

C

A

G

F

T

I

V

S

L

I

F

L

I

S

V

A

Q

I

T

I

I

I

L

H

Y

I

T

A

G

F

I

S

V

F

I

L

Y

A

A

G

P

G

A

I

L

V

A

L

L

E

N

T

Q

L

V

F

T

G

G

I

F

D

D

M

L

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W

V

G

S

A

I

V

V

V

V

A

I

T

A

G

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V

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C

G

T

L

F

Y

Y

T

C

I

T

I

L

G

G

L

L

R

K

A

A

V

V

V

I

V

W

T

T

E

D

T

V

I

F

Q

Q

S
x
V

L

G

V

I

L

M

I

V

T

A

G

G

A

F

I

A

I

S

I

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I

F

Q

A

A

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V

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M

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Q

G

G

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-

D

-

H

-

M

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A

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K

D

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A

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A

D

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D

G

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F

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-

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N

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P

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W

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A

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V

I

F

-

L

-

G

-

Y

-

P

-

V

I

L

G

G

G

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x
F

Y

T

W

W

C

T

A

S

-

I

-

Y

-

G

-

V

D

N

Q

Q

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Q

Q

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A

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G

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A

A

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G

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D

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K

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V

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S

A

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P

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N

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D

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Q

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G

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P

G

G

I

L

L

F

V

V

A

A

A

C

L

A

L

Y

S

S

G

G

L

T

M

L

S

S

Q

T

I

V

A

S

G

S

A

S

L

I

N

N

S

A

I

L

A

A

T

A

L

V

S

T

S

V

Y

E

D

D

L

L

Y

I

K

K

R

P

F

Y

K

F

P

R

E

S

T

L

S

S

D

E

K

R

K

S

L

L

V

S

S

W

V

I

G

S

R

Q

W

G

S

M

A

S

G

V

I

V

A

Y

L

G

T

A

V

L

S

C

I

I

G

G

L

M

L

A

P

A

L

L

L

A

N

S

S

L

Y

M

E

G

S

A

L

L

F

L

N

Q

G

A

I

A

N

L

D

S

V

V

I

F

A

G

H

M

I

V

A

G

P

G

P

P

I

L

T

M

C

G

V

L

F

F

L

A

L

L

G

G

V

I

F

L

W

V

K

P

K

F

A

A

S

N

A

S

K

I

G

G

A

A

Q

L

Y

V

T

G

L

L

L

M

L

A

G

G

S

F

I

A

I

I

G

S

A

L

G

W

V

V

F

G

V

I

V

G

N

A

K

Q

V

I

Y

Y

V193 (≠ S188) to I: in dbSNP:rs1709189
F251 (≠ W252) to V: in dbSNP:rs11834933

Sites not aligning to the query:

608 T→A: Loss of interaction with PDZK1.
608:610 PDZ-binding
610 L→A: Loss of interaction with PDZK1.

Query Sequence

>CA265_RS04220 FitnessBrowser__Pedo557:CA265_RS04220
MISTTDIVITIAYILFIVTIGLWTGTRKKKNEETTSGEYFLAGKTLKWPMIGLALFATNI
SCLHLVSLAQSGFDSGLLNGNFEWMAAFTLILLALLFIPFYIRSGISTLPDFLERRYNRA
CRDWLAFISILSAIIIHIAFSFLAGGIVLETLFGIDMYVSIVVIALLTGLYTIIGGLRAV
VVTETIQSLVLITGAIIITYFAWNKVGGWDHMTAILQKENAMDKLSMIRPIGDKSGMSWI
AVFLGYPVLGIWYWCADQTIVQRVLGAKDENHARVGSLFCGFIKILPVFIFVLPGLFAYI
LYKSGTMDLSSLQTVGSNGETVLNTKGIYTLMITQLLPKGLVGILVAALLSGLMSQIAGA
LNSIATLSSYDLYKRFKPETSDKKLVSVGRWSAGIALTVSIGLLPLLNSYESLFNGINDV
IAHIAPPITCVFLLGVFWKKASAKGAQYTLLLGSIIGAGVFVVNKVYGTETIIGQIPFMM
MAFYLFCICVLIQVVFSHIYPVKHTAQSETLYWTSIWEPLKSKGWSGIGNYKFLSVLLLA
IMGVLYVYFK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory