SitesBLAST
Comparing CA265_RS04220 FitnessBrowser__Pedo557:CA265_RS04220 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
P11170 Sodium/glucose cotransporter 1; Na(+)/glucose cotransporter 1; High affinity sodium-glucose cotransporter; Solute carrier family 5 member 1 from Oryctolagus cuniculus (Rabbit) (see 2 papers)
33% identity, 88% coverage: 3:486/550 of query aligns to 25:534/662 of P11170
- C255 (vs. gap) modified: Disulfide link with 608
- Q457 (≠ N418) mutation to W: Drasticly decreased affinity for glucose and phlorizin.
- T460 (≠ I421) mutation to W: Decreased affinity for glucose and phlorizin.
Sites not aligning to the query:
- 608 modified: Disulfide link with 255
Q9NY91 Probable glucose sensor protein SLC5A4; Solute carrier family 5 member 4 from Homo sapiens (Human) (see paper)
31% identity, 85% coverage: 3:469/550 of query aligns to 25:508/659 of Q9NY91
- E457 (≠ N418) mutation to Q: Confers sugar transport activity not found in the wild-type protein. Increased sensitivity to inhibitor phlorizin.
7wmvA Structure of human sglt1-map17 complex bound with lx2761 (see paper)
33% identity, 90% coverage: 3:497/550 of query aligns to 8:528/602 of 7wmvA
- binding N-[2-(dimethylamino)ethyl]-2-methyl-2-[4-[4-[[2-methyl-5-[(2S,3R,4R,5S,6R)-6-methylsulfanyl-3,4,5-tris(oxidanyl)oxan-2-yl]phenyl]methyl]phenyl]butanoylamino]propanamide: N61 (= N59), H66 (= H64), L70 (= L68), I81 (≠ N79), F84 (= F82), L257 (≠ M237), M266 (≠ Y246), L269 (= L249), T270 (≠ G250), Y273 (= Y253), W274 (= W254), F436 (= F414), D437 (≠ N415), Q440 (≠ N418), H508 (≠ P477)
P13866 Sodium/glucose cotransporter 1; Na(+)/glucose cotransporter 1; High affinity sodium-glucose cotransporter; Solute carrier family 5 member 1 from Homo sapiens (Human) (see 7 papers)
33% identity, 90% coverage: 3:497/550 of query aligns to 25:545/664 of P13866
- N51 (≠ K29) to S: in GGM; slightly decreased activity; dbSNP:rs17683011
- W67 (= W48) mutation to A: Strong reduction in D-glucose transporter activity.
- S77 (≠ T58) mutation to A: Loss of activity.
- H83 (= H64) mutation to L: Acquires D-mannose, D-fructose and L-sorbose transporter activity; when associated with A-287 and C-290.; mutation to Q: Loss of D-glucose transporter activity.
- R135 (= R116) to W: in GGM; loss of activity
- S159 (≠ A139) to P: in GGM; loss of activity; dbSNP:rs933026071
- A166 (≠ G146) to T: in GGM; about 90% reduction in activity
- D204 (≠ E184) mutation to A: Loss of activity.
- N248 (= N220) modified: carbohydrate, N-linked (GlcNAc...) asparagine; mutation to Q: Loss of N-glycosylation.
- C255 (≠ M227) modified: Disulfide link with 511
- W276 (= W239) to L: in GGM; about 95% reduction in activity
- T287 (≠ G250) mutation to A: Acquires D-mannose, D-fructose and L-sorbose transporter activity; when associated with L-83 and C-290.; mutation to N: Loss of D-glucose transporter activity. Has strict selectivity for D-galactose.; mutation T->S,A: Has normal D-glucose and D-galactose transporter activity.
- Y290 (= Y253) mutation to C: Loss of D-galactose transporter activity. Has strict selectivity for D-glucose. Acquires D-mannose, D-fructose and L-sorbose transporter activity; when associated with A-287 and L-83.
- W291 (= W254) mutation to A: Loss of D-glucose transporter activity.
- C292 (= C255) to Y: in GGM; loss of activity; dbSNP:rs765502638; mutation to A: Has no effect on water permeability.
- Q295 (= Q258) to R: in GGM; loss of activity; dbSNP:rs779428134
- R300 (= R263) to S: in GGM; loss of activity
- A304 (= A267) to V: in GGM; impairs trafficking to the plasma membrane
- K321 (= K284) mutation to Q: Acquires D-mannose and D-allose transporter activity comparable to glucose and galactose.
- C345 (≠ D308) modified: Disulfide link with 351
- C351 (≠ T314) modified: Disulfide link with 345
- C355 (≠ N318) modified: Disulfide link with 361
- C361 (≠ N324) modified: Disulfide link with 355
- N363 (≠ K326) mutation to A: Loss of water permeation.
- L369 (≠ M332) to S: in GGM; loss of activity
- R379 (≠ V342) to Q: in GGM; loss of activity; dbSNP:rs747215838
- A388 (≠ S351) to V: in GGM; loss of activity
- S396 (≠ G359) mutation to A: Loss of activity.
- F405 (≠ S368) to S: in GGM; loss of activity
- A411 (≠ K374) to T: in GGM; slightly decreased activity; dbSNP:rs17683430
- G426 (= G389) to R: in GGM; loss of activity; dbSNP:rs1304151494
- Q451 (vs. gap) mutation to A: Strong reduction in water permeation.
- L452 (= L413) mutation to A: Loss of water permeation.
- D454 (≠ N415) mutation to A: Has no effect on water permeation.
- Q457 (≠ N418) mutation to A: Loss of D-glucose transporter activity.; mutation to C: Strong reduction in D-glucose transporter activity.
- T460 (≠ I421) mutation to A: Loss of D-glucose transporter activity.
- V470 (= V431) to N: in GGM; about 90% reduction in activity; requires 2 nucleotide substitutions
- R499 (≠ V460) to H: in GGM; impairs trafficking to the plasma membrane; decreases the sugar affinity; dbSNP:rs927157864
- C511 (vs. gap) modified: Disulfide link with 255
- C517 (vs. gap) modified: Disulfide link with 522
- C522 (≠ G474) modified: Disulfide link with 517
Sites not aligning to the query:
- 191:664 natural variant: Missing (in GGM; loss of activity)
- 379:664 natural variant: Missing (in GGM; loss of activity)
- 615 H → Q: in GGM; slightly decreased activity; dbSNP:rs33954001
- 641 W→A: Slightly reduced D-glucose transporter activity.
- 660:661 HA→WG: Loss of D-glucose transporter activity.
7slaA Cryoem structure of sglt1 at 3.15 angstrom resolution (see paper)
31% identity, 99% coverage: 3:549/550 of query aligns to 7:576/585 of 7slaA
7sl8A Cryoem structure of sglt1 at 3.4 a resolution (see paper)
31% identity, 99% coverage: 3:549/550 of query aligns to 6:573/582 of 7sl8A
8hezA Structure of human sglt2-map17 complex with dapagliflozin (see paper)
33% identity, 81% coverage: 3:446/550 of query aligns to 2:465/582 of 8hezA
- binding (2S,3R,4R,5S,6R)-2-[4-chloranyl-3-[(4-ethoxyphenyl)methyl]phenyl]-6-(hydroxymethyl)oxane-3,4,5-triol: N55 (= N59), G59 (≠ L63), H60 (= H64), G63 (≠ S67), L64 (= L68), T67 (≠ S71), F78 (= F82), E79 (= E83), V266 (≠ L249), S267 (≠ G250), W271 (= W254), K301 (= K284), F433 (= F414), Q437 (≠ N418)
- binding sodium ion: A53 (= A57), I56 (= I60), G57 (≠ S61), A369 (≠ G352), S372 (= S355), S373 (≠ Q356)
Sites not aligning to the query:
8hdhA Structure of human sglt2-map17 complex with canagliflozin (see paper)
33% identity, 81% coverage: 3:446/550 of query aligns to 2:465/586 of 8hdhA