SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing CA265_RS04225 FitnessBrowser__Pedo557:CA265_RS04225 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q7L5Y1 Mitochondrial enolase superfamily member 1; Antisense RNA to thymidylate synthase; rTS; L-fuconate dehydratase; EC 4.2.1.68 from Homo sapiens (Human) (see 5 papers)
41% identity, 100% coverage: 2:406/406 of query aligns to 6:419/443 of Q7L5Y1

query
sites
Q7L5Y1

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M145 (≠ E138) to T: in dbSNP:rs2612086
S148 (≠ N141) modified: Phosphoserine
D250 (= D243) binding
E276 (= E269) binding
E305 (= E296) binding

Sites not aligning to the query:

1:27 mutation Missing: Impairs protein solubility. Abolishes catalytic activity.

4a35A Crystal structure of human mitochondrial enolase superfamily member 1 (enosf1) (see paper)
41% identity, 100% coverage: 2:406/406 of query aligns to 7:420/441 of 4a35A

query
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4a35A

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active site: K221 (= K212), K223 (= K214), D251 (= D243), N253 (= N245), E277 (= E269), E306 (= E296), D329 (= D319), H356 (= H346), S380 (vs. gap), E387 (= E373)
binding magnesium ion: Y188 (= Y179), D251 (= D243), E277 (= E269), E306 (= E296), Y374 (≠ H362)

4ip5A Crystal structure of l-fuconate dehydratase from silicibacter sp. Tm1040 liganded with mg and d-erythronohydroxamate
41% identity, 100% coverage: 2:406/406 of query aligns to 3:412/421 of 4ip5A

query
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4ip5A

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active site: K217 (= K214), D245 (= D243), N247 (= N245), E271 (= E269), E298 (= E296), D321 (= D319), H348 (= H346), E379 (= E373)
binding (2r,3r)-n,2,3,4-tetrahydroxybutanamide: G21 (= G19), D23 (= D21), Y31 (= Y29), K215 (= K212), K217 (= K214), D245 (= D243), N247 (= N245), E298 (= E296), H348 (= H346)
binding magnesium ion: D245 (= D243), E271 (= E269), E298 (= E296)

4ip4A Crystal structure of l-fuconate dehydratase from silicibacter sp. Tm1040 liganded with mg
41% identity, 100% coverage: 2:406/406 of query aligns to 3:412/421 of 4ip4A

query
sites
4ip4A

I

I

N

T

K

G

I

L

E

R

I

T

S

Y

D

D

K

L

R

R

F

F

E

P

L

T

S

S

T

E

G

G

A

L

-

D

G

G

S

S

D

D

A

A

I

M

H

N

K

P

D

D

P

P

Q

D

Y

Y

S

S

Y

A

A

A

V

Y

T

V

N

I

L

L

T

E

N

T

E

E

N

G

G

T

I

H

T

E

G

G

T

H

G

G

L

L

A

T

F

F

T

T

L

I

G

G

A

R

G

G

N

N

D

E

L

I

V

C

C

I

N

A

A

A

A

I

Q

K

F

A

Y

L

A

G

N

A

K

L

L

V

K

V

G

G

Q

L

D

D

I

L

E

D

E

W

L

I

M

R

S

E

D

D

F

M

G

G

Q

R

T

F

F

W

R

R

T

H

L

V

S

T

N

G

E

D

Q

S

Q

Q

F

L

R

R

W

W

L

I

G

G

P

P

H

D

K

K

G

G

V

A

V

I

H

H

L

L

G

A

L

A

A

G

S

A

V

V

T

V

N

N

A

A

C

V

Y

W

D

D

L

L

W

W

A

A

K

K

K

D

R

T

G

G

V

K

P

P

L

V

W

W

K

R

L

L

L

V

I

A

D

D

L

M

S

S

P

P

E

A

E

E

I

I

V

L

N

R

T

L

L

I

D

D

L

F

S

R

Y

Y

L

L

E

T

D

D

V

V

L

L

T

A

K

P

E

E

A

A

I

L

A

D

M

L

L

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A

Q

A

T

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D

P

S

G

K

K

K

E

S

E

R

R

E

I

G

A

-

R

I

L

L

I

D

E

T

E

G

G

Y

Y

P

P

G

C

Y

Y

D

T

T

T

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S

V

A

G

G

W

W

F

L

N

G

Y

Y

D

S

D

D

E

E

K

K

V

L

R

R

E

R

N

L

C

C

K

R

K

E

A

A

I

R

A

A

N

A

G

G

F

F

T

T

A

H

M

T

K

K

L

F

K
|
K

V

V

G

G

S

R

A

-

D

D

P

L

K

S

R

D

D

D

I

I

R

R

A

L

N

T

I

I

V

A

R

R

E

E

V

E

A

L

G

G

E

E

S

D

S

M

K

N

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I

M

M

L

I

D
|
D

A

A

N
|
N

Q

Q

Q

V

W

W

T

E

L

V

P

D

Q

E

A

A

I

I

S

D

I

W

C

V

N

N

E

R

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L

K

A

N

F

M

A

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R

P

P

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L

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I

E
|
E

E

E

P

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P

P

D

D

V

V

L

L

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H

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T

A

L

I

A

R

R

E

E

G

I

V

A

A

P

P

V

I

K

K

L

V

A

A

L

T

G

G

E
|
E

H

M

V

C

P

Q

N

N

R

R

I

I

I

M

F

F

K

K

N

Q

Y

F

L

I

Q

A

T

S

G

G

C

A

T

L

G

D

F

I

A

V

Q

Q

V

I

D
|
D

A

S

V

C

R

R

V

M

G

G

V

L

S

N

E

E

F

V

I

L

T

A

I

V

S

M

L

L

L

V

C

A

K

A

K

K

F

Y

G

D

V

L

P

P

V

V

V

W

P

P

H
|
H

V

A

G

G

D

G

M

V

G

G

-

L

-

C

Q

E

L

Y

H

V

Q

Q

H

H

L

M

V

S

L

M

F

I

N

D

H

Y

I

V

A

A

M

I

G

C

H

G

E

E

-

K

-

D

A

S

L

K

F

R

L

I

E
|
E

H

Y

I

V

P

D

H

H

L

L

K

H

Q

E

H

H

F

F

K

K

H

H

P

P

I

C

K

I

I

V

E

T

K

G

G

G

V

A

Y

Y

I

Q

T

A

P

P

Q

H

E

A

A

P

G

G

S

F

S

S

C

I

D

E

L

M

K

K

active site: K217 (= K214), D245 (= D243), N247 (= N245), E271 (= E269), E298 (= E296), D321 (= D319), H348 (= H346), E379 (= E373)
binding magnesium ion: D245 (= D243), E271 (= E269), E298 (= E296)

Q8P3K2 L-fuconate dehydratase; FucD; EC 4.2.1.68 from Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25) (see paper)
41% identity, 100% coverage: 2:405/406 of query aligns to 4:414/441 of Q8P3K2

query
sites
Q8P3K2

I

I

N

I

K

A

I

L

E

E

I

T

S

H

D

D

K

V

R

R

F

F

E

P

L

T

S

S

T

R

G

E

A

L

-

D

G
|
G

S
|
S

D
|
D

A

A

I

M

H

N

K

P

D

D

P

P

Q

D

Y
|
Y

S

S

-

A

Y

Y

A

V

V

V

T

L

N

R

L

T

T

D

N

G

E

A

N

E

G

D

I

L

T

A

G

G

T

Y

G

G

L

L

A

V

F

F

T

T

L

I

G

G

A

R

G

G

N

N

D

D

L

V

V

Q

C

T

N

A

A

A

A

V

Q

A

F

A

Y

L

A

A

N

E

K

H

L

V

K

V

G

G

Q

L

D

S

I

V

E

D

E

K

L

V

M

I

S

A

D

D

F

L

G

G

Q

A

T

F

F

A

R

R

T

R

L

L

S

T

N

N

E

D

Q

S

Q

Q

F

L

R

R

W

W

L

L

G

G

P

P

H

E

K

K

G

G

V

V

V

M

H

H

L

M

G

A

L

I

A

G

S

A

V

V

T

I

N

N

A

A

C

A

Y

W

D

D

L

L

W

A

A

A

K

R

K

A

R

A

G

N

V

K

P

P

L

L

W

W

K

R

L

F

L

I

I

A

D

E

L

L

S

T

P

P

E

E

E

Q

I

L

V

V

N

D

T

T

L

I

D

D

L

F

S

R

Y

Y

L

L

E

S

D

D

V

A

L

L

T

T

K

R

E

D

E

E

A

A

I

L

A

A

M

I

L

L

Q

R

S

D

Q

A

T

Q

D

P

S

Q

K

R

K

A

S

A

R

R

E

T

G

A

-

T

I

L

L

I

D

E

T

Q

G

G

Y

Y

P

P

G

A

Y

Y

D

T

T

T

S

S

V

P

G

G

W

W

F

L

N

G

Y

Y

D

S

D

D

E

E

K

K

V

L

R

V

E

R

N

L

C

A

K

K

K

E

A

A

I

V

A

A

N

D

G

G

F

F

T

R

A

T

M

I

K
|
K

L

L

K
|
K

V

V

G

G

S

-

A

A

D

N

P

V

K

Q

R

D

D

D

I

I

R

R

A

C

N

R

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A

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R

E

A

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A

A

I

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G

E

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D

S

I

K

A

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M

M

A

L

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D
|
D

A

A

N
|
N

Q

Q

Q

R

W

W

T

D

L

V

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G

Q

P

A

A

I

I

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I

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M

N

R

E

Q

L

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K

A

N

E

M

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N

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E
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E

E

E

P

P

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P

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D

V

V

L

L

A

G

H

H

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A

T

A

L

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A

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R

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E

G

I

I

A

T

P

P

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V

K

P

L

V

A

S

L

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G

G

E
|
E

H

H

V

T

P

Q

N

N

R

R

I

V

F

F

K

K

N

Q

Y

L

L

L

Q

Q

T

A

G

G

C

A

T

V

G

D

F

L

A

I

Q

Q

V

I

D

D

A

A

V

A

R

R

V

V

G

G

V

V

S

N

E

E

F

N

I

L

T

A

I

I

S

L

L

L

C

A

K

A

K

K

F

F

G

G

V

V

P

R

V

V

V

F

P

P

H
|
H

V
x
A

G
|
G

D

G

M

V

G

G

-

L

-

C

Q

E

L

L

H

V

Q

Q

H

H

L

L

V

A

L

M

F

A

N

D

H

F

I

V

A

A

M

I

G

T

-

G

-

K

H

M

E

E

A

D

L

R

F

A

L

I

E
|
E

H

F

I

V

P

D

H

H

L

L

K

H

Q

Q

H

H

F

F

K

L

H

D

P

P

I

V

K

R

I

I

E

Q

K

H

G

G

V

R

Y

Y

I

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T

A

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P

Q

E

E

V

A

P

G

G

S

F

S

S

C

A

D

E

L

M

GSD 22:24 (= GSD 19:21) binding
Y32 (= Y29) binding
K218 (= K212) binding
K220 (= K214) mutation to A: Inactive.
D248 (= D243) binding
N250 (= N245) binding
E274 (= E269) binding ; binding
E301 (= E296) binding ; binding
HAG 351:353 (≠ HVG 346:348) binding
E382 (= E373) binding

1yeyC Crystal structure of l-fuconate dehydratase from xanthomonas campestris pv. Campestris str. Atcc 33913
41% identity, 100% coverage: 2:405/406 of query aligns to 4:414/435 of 1yeyC

query
sites
1yeyC

I

I

N

I

K

A

I

L

E

E

I

T

S

H

D

D

K

V

R

R

F

F

E

P

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T

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S

T

R

G

E

A

L

-

D

G

G

S

S

D

D

A

A

I

M

H

N

K

P

D

D

P

P

Q

D

Y

Y

S

S

-

A

Y

Y

A

V

V

V

T

L

N

R

L

T

T

D

N

G

E

A

N

E

G

D

I

L

T

A

G

G

T

Y

G

G

L

L

A

V

F

F

T
|
T

L

I

G

G

A

R

G

G

N

N

D

D

L

V

V

Q

C

T

N

A

A

A

A

V

Q

A

F

A

Y

L

A

A

N

E

K

H

L

V

K

V

G

G

Q

L

D

S

I

V

E

D

E

K

L

V

M

I

S

A

D

D

F

L

G

G

Q

A

T

F

F

A

R

R

T

R

L

L

S

T

N

N

E

D

Q

S

Q

Q

F

L

R

R

W

W

L

L

G

G

P

P

H

E

K

K

G

G

V

V

V

M

H

H

L

M

G

A

L

I

A

G

S

A

V

V

T

I

N

N

A

A

C

A

Y

W

D

D

L

L

W

A

A

A

K

R

K

A

R

A

G

N

V

K

P

P

L

L

W

W

K

R

L

F

L

I

I

A

D

E

L

L

S

T

P

P

E

E

E

Q

I

L

V

V

N

D

T

T

L

I

D

D

L

F

S

R

Y

Y

L

L

E

S

D

D

V

A

L

L

T

T

K

R

E

D

E

E

A

A

I

L

A

A

M

I

L

L

Q

R

S

D

Q

A

T

Q

D

P

S

Q

K

R

K

A

S

A

R

R

E

T

G

A

-

T

I

L

L

I

D

E

T

Q

G

G

Y

Y

P

P

G

A

Y

Y

D

T

T
|
T

S

S

V

P

G

G

W

W

F

L

N

G

Y

Y

D

S

D

D

E

E

K

K

V

L

R

V

E

R

N

L

C

A

K

K

K

E

A

A

I

V

A

A

N

D

G

G

F

F

T

R

A

T

M

I

K
|
K

L

L

K
|
K

V

V

G

G

S

-

A

A

D

N

P

V

K

Q

R

D

D

D

I

I

R

R

A

C

N

R

I

L

V

A

R

R

E

A

V

A

A

I

G

G

E

P

S

D

S

I

K

A

V

M

M

A

L

V

D
|
D

A

A

N
|
N

Q

Q

Q

R

W

W

T

D

L

V

P

G

Q

P

A

A

I

I

S

D

I

W

C

M

N

R

E

Q

L

L

K

A

N

E

M

F

N

D

P

I

F

A

W

W

V

I

E
|
E

E

E

P

P

T

T

H

S

P

P

D

D

V

V

L

L

A

G

H

H

Q

A

T

A

L

I

A

R

R

Q

E

G

I

I

A

T

P

P

V

V

K

P

L

V

A

S

L

T

G
|
G

E
|
E

H

H

V

T

P

Q

N

N

R

R

I

V

F

F

K

K

N

Q

Y

L

L

L

Q

Q

T

A

G

G

C

A

T

V

G

D

F

L

A

I

Q

Q

V

I

D
|
D

A

A

V

A

R

R

V

V

G

G

V

V

S

N

E

E

F

N

I

L

T

A

I

I

S

L

L

L

C

A

K

A

K

K

F

F

G

G

V

V

P

R

V

V

V

F

P
|
P

H
|
H

V
x
A

G

G

D

G

M

V

G

G

-

L

-

C

Q

E

L

L

H

V

Q

Q

H

H

L

L

V

A

L

M

F

A

N
x
D

H

F

I

V

A

A

M

I

G

T

-

G

-
x
K

H

M

E

E

A

D

L

R

F

A

L

I

E
|
E

H

F

I

V

P

D

H

H

L

L

K

H

Q

Q

H

H

F

F

K

L

H

D

P

P

I

V

K

R

I

I

E

Q

K

H

G

G

V

R

Y

Y

I

L

T

A

P

P

Q

E

E

V

A

P

G

G

S

F

S

S

C

A

D

E

L

M

active site: T55 (= T50), T190 (= T184), K218 (= K212), K220 (= K214), D248 (= D243), N250 (= N245), E274 (= E269), G300 (= G295), E301 (= E296), D324 (= D319), P350 (= P345), H351 (= H346), A352 (≠ V347), D368 (≠ N361), K375 (vs. gap), E382 (= E373)
binding magnesium ion: D248 (= D243), E274 (= E269), E301 (= E296)

2hxtA Crystal structure of l-fuconate dehydratase from xanthomonas campestris liganded with mg++ and d-erythronohydroxamate (see paper)
41% identity, 100% coverage: 2:405/406 of query aligns to 3:413/434 of 2hxtA

query
sites
2hxtA

I

I

N

I

K

A

I

L

E

E

I

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H

D

D

K

V

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S

T

R

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E

A

L

-

D

G
|
G

S

S

D
|
D

A

A

I

M

H

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K

P

D

D

P

P

Q

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Y
|
Y

S

S

-

A

Y

Y

A

V

V

V

T

L

N

R

L

T

T

D

N

G

E

A

N

E

G

D

I

L

T

A

G

G

T

Y

G

G

L

L

A

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F

F

T
|
T

L

I

G

G

A

R

G

G

N

N

D

D

L

V

V

Q

C

T

N

A

A

A

A

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Q

A

F

A

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L

A

A

N

E

K

H

L

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K

V

G

G

Q

L

D

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I

V

E

D

E

K

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I

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A

D

D

F

L

G

G

Q

A

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F

F

A

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R

L

L

S

T

N

N

E

D

Q

S

Q

Q

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L

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R

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W

L

L

G

G

P

P

H

E

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K

G

G

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M

H

H

L

M

G

A

L

I

A

G

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A

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T

I

N

N

A

A

C

A

Y

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D

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L

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A

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K

A

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A

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L

I

I

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P

E

E

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N

D

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A

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E

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E

A

A

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A

A

M

I

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D

Q

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A

S

A

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G

A

-

T

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I

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G

Y

Y

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G

A

Y

Y

D

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T

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G

G

W

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F

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G

Y

Y

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D

E

E

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R

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L

C

A

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E

A

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A

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R

A

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K

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K
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K

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V

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G

S

-

A

A

D

N

P

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R

D

D

D

I

I

R

R

A

C

N

R

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I

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A

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M

A

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D

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|
N

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A

A

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E

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A

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R

Q

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I

I

A

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P

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P

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A

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E
|
E

H

H

V

T

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Q

N

N

R

R

I

V

F

F

K

K

N

Q

Y

L

L

L

Q

Q

T

A

G

G

C

A

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G

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A

I

Q

Q

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I

D
|
D

A

A

V

A

R

R

V

V

G

G

V

V

S

N

E

E

F

N

I

L

T

A

I

I

S

L

L

L

C

A

K

A

K

K

F

F

G

G

V

V

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R

V

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V

F

P
|
P

H
|
H

V
x
A

G
|
G

D

G

M

V

G

G

-

L

-

C

Q

E

L

L

H

V

Q

Q

H

H

L

L

V

A

L

M

F

A

N
x
D

H

F

I

V

A

A

M

I

G

T

-

G

-
x
K

H

M

E

E

A

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F

A

L

I

E
|
E

H

F

I

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P

D

H

H

L

L

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H

Q

Q

H

H

F

F

K

L

H

D

P

P

I

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I

I

E

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K

H

G

G

V

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Y

Y

I

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C

A

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E

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M

active site: T54 (= T50), T189 (= T184), K217 (= K212), K219 (= K214), D247 (= D243), N249 (= N245), E273 (= E269), G299 (= G295), E300 (= E296), D323 (= D319), P349 (= P345), H350 (= H346), A351 (≠ V347), D367 (≠ N361), K374 (vs. gap), E381 (= E373)
binding (2r,3r)-n,2,3,4-tetrahydroxybutanamide: G21 (= G19), D23 (= D21), Y31 (= Y29), K217 (= K212), K219 (= K214), E300 (= E296), H350 (= H346), G352 (= G348), E381 (= E373)
binding magnesium ion: D247 (= D243), E273 (= E269), E300 (= E296)

2hxuA Crystal structure of k220a mutant of l-fuconate dehydratase from xanthomonas campestris liganded with mg++ and l-fuconate (see paper)
41% identity, 100% coverage: 2:405/406 of query aligns to 3:413/434 of 2hxuA

query
sites
2hxuA

I

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-

D

G
|
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S

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D
|
D

A

A

I

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H

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P

D

D

P

P

Q

D

Y
|
Y

S

S

-

A

Y

Y

A

V

V

V

T

L

N

R

L

T

T

D

N

G

E

A

N

E

G

D

I

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A

G

G

T

Y

G

G

L

L

A

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F

T
|
T

L

I

G

G

A

R

G

G

N

N

D

D

L

V

V

Q

C

T

N

A

A

A

A

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Q

A

F

A

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A

A

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E

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K

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I

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D

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A

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D

F

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G

Q

A

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R

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N

N

E

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Q

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Q

Q

F

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W

W

L

L

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E

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G

G

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H

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A

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I

A

G

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A

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I

N

N

A

A

C

A

Y

W

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D

L

L

W

A

A

A

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A

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A

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F

L

I

I

A

D

E

L

L

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E

E

E

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I

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V

N

D

T

T

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D

D

L

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Y

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D

V

A

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A

A

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I

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A

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A

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A

-

T

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I

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G

Y

Y

P

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G

A

Y

Y

D

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T
|
T

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G

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W
|
W

F

L

N

G

Y

Y

D

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D

E

E

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E

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N

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A

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D

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|
K

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x
A

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A

A

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A

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D

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E

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G

E
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E

H

H

V

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Q

N

N

R

R

I

V

F

F

K

K

N

Q

Y

L

L

L

Q

Q

T

A

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G

C

A

T

V

G

D

F

L

A

I

Q

Q

V

I

D
|
D

A

A

V

A

R

R

V

V

G

G

V

V

S

N

E

E

F

N

I

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A

I

I

S

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A

K

A

K

K

F

F

G

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P

H
|
H

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x
A

G
|
G

D

G

M

V

G

G

-

L

-

C

Q

E

L

L

H

V

Q

Q

H

H

L

L

V

A

L

M

F

A

N
x
D

H

F

I

V

A

A

M

I

G

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-

G

-
x
K

H

M

E

E

A

D

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F

A

L

I

E
|
E

H

F

I

V

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D

H

H

L

L

K

H

Q

Q

H

H

F

F

K

L

H

D

P

P

I

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R

I

I

E

Q

K

H

G

G

V

R

Y

Y

I

L

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A

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active site: T54 (= T50), T189 (= T184), K217 (= K212), A219 (≠ K214), D247 (= D243), N249 (= N245), E273 (= E269), G299 (= G295), E300 (= E296), D323 (= D319), P349 (= P345), H350 (= H346), A351 (≠ V347), D367 (≠ N361), K374 (vs. gap), E381 (= E373)
binding 6-deoxy-L-galactonic acid: G21 (= G19), D23 (= D21), Y31 (= Y29), W193 (= W188), K217 (= K212), D247 (= D243), E300 (= E296), H350 (= H346), G352 (= G348), E381 (= E373)
binding magnesium ion: D247 (= D243), E273 (= E269), E300 (= E296)

4h19A Crystal structure of an enolase (mandelate racemase subgroup, target efi-502087) from agrobacterium tumefaciens, with bound mg and d- ribonohydroxamate, ordered loop
30% identity, 99% coverage: 2:402/406 of query aligns to 3:356/372 of 4h19A

query
sites
4h19A

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A

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H
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H

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Y

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Y

V

A

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G

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A

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D

G

G

I

I

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G

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G

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|
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-
x
H

-

A

-

H

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L

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D

L

L

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Y

A

A

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-

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A

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H

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A

L

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A

A

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A

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D

N

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A

A

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D

D

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I

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A

A

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A

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L

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I

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-

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-

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A

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A

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E

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D

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G

G

V

V

Y

Y

I

K

T

R

P

P

Q

E

E

Q

A

P

G

G

S

A

S

S

active site: I20 (≠ G19), T51 (= T50), T143 (= T184), K172 (= K212), K174 (= K214), D203 (= D243), N205 (= N245), E229 (= E269), G254 (= G295), E255 (= E296), Q276 (= Q317), D278 (= D319), H305 (= H346), A306 (≠ V347), G307 (= G348), E327 (= E373)
binding (2R,3R,4R)-N,2,3,4,5-pentakis(oxidanyl)pentanamide: D22 (= D21), H25 (= H24), H52 (vs. gap), K172 (= K212), K174 (= K214), D203 (= D243), N205 (= N245), E229 (= E269), E255 (= E296), H305 (= H346), E327 (= E373)
binding calcium ion: D268 (≠ Q309), H298 (≠ F339)
binding magnesium ion: D203 (= D243), E229 (= E269), E255 (= E296)

2pp1A Crystal structure of l-talarate/galactarate dehydratase from salmonella typhimurium lt2 liganded with mg and l-lyxarohydroxamate (see paper)
25% identity, 88% coverage: 33:391/406 of query aligns to 61:363/395 of 2pp1A

query
sites
2pp1A

V

I

T

A

N

E

L

I

T

R

N

S

E

R

N

D

G

G

I

F

T

E

G

G

T

V

G

G

L

F

A

S

F

Y

T
x
S

L
x
K

G

R

A

A

G

G

N

G

D

Q

L

G

V

I

C

Y

N

A

A

H

A

A

Q

K

F

E

Y

I

A

A

N

D

K

N

L

L

K

L

G

G

Q

E

D

D

I

P

E

N

E

D

L

I

M

D

S

K

D

I

F

Y

G

T

Q

K

T

L

F

L

R

-

T

-

L

-

S

-

N

-

E

-

Q

-

F

-

R

-

W

W

L

A

G

G

P

A

H

S

K

V

G

G

V

R

V

S

H

G

L

M

G

A

L

V

A

Q

S

A

V

I

T

S

N

P

-

I

-

D

-

I

A

A

C

L

Y

W

D

D

L

M

W

K

A

A

K

K

K

R

R

A

G

G

V

L

P

P

L

L

W

A

K

K

L

L

I

-

D

-

L

-

S

-

P

-

E

-

I

-

V

-

N

-

T

-

L

-

D

-

L

-

S

-

Y

-

L

-

E

-

D

-

V

-

L

-

T

-

K

-

E

-

A

-

I

-

A

-

M

-

L

-

Q

G

S

A

Q

H

T

R

D

D

S

S

K

V

K

Q

S

C

R

-

E

-

G

-

I

-

L

-

D

-

T

-

G

-

Y

-

P

-

G

-

Y

Y

D

N

T

T

S

S

V

G

G

G

W
x
F

F

L

N

H

Y

T

D

P

D

L

E

D

K

Q

V

V

R

L

E

K

N

N

C

V

K

V

K

I

A

S

I

R

A

E

N

N

G

G

F

I

T

G

A

G

M

I

K
|
K

L

L

K
|
K

V

V

G

G

S

Q

A

P

D

N

P

C

K

A

R

E

D

D

I

I

R

R

A

L

N

T

I

A

V

V

R

R

E

E

V

A

A

L

G

G

E

D

S

E

S

F

K

P

V

L

M

M

L

V

D
|
D

A

A

N
|
N

Q

Q

W

W

T

D

L

R

P

E

Q

T

A

A

I

I

S

R

I

M

C

G

N

R

E

K

L

M

K

E

N

Q

M

F

N

N

P

L

F

I

W

W

V

I

E
|
E

E

E

P

P

T

L

H

D

P

A

D

Y

D

D

V

I

L

E

A

G

H

H

Q

A

T

Q

L

L

A

A

R

A

E

A

I

L

A

-

P

D

V

T

K

P

L

I

A

A

L

T

G
|
G

E
|
E

H

M

V

L

P

T

N

S

R

F

I

R

I

E

F

H

K

E

N

Q

Y

L

L

I

Q

L

T

G

G

N

C

A

T

S

G

D

F

F

A

V

Q

Q

V

P

D
|
D

A

A

V

P

R

R

V

V

G

G

V

I

S

S

E

P

F

F

I

L

T

K

I

I

S

M

L

D

L

L

C

A

K

A

K

K

F

H

G

G

V

R

P

K

V

L

V

A

P

P

H
|
H

V

F

G

A

D

-

M

-

G

M

Q

E

L

V

H

H

Q

L

H

H

L

L

V

S

L

A

F

A

N

Y

H

P

I

L

A

E

M

P

G

-

H

-

E

-

A

-

L

-

F

W

L

L

E
|
E

H

H

I

F

P

E

H

W

L

L

K

N

Q

P

H

L

F

F

K

N

H

E

P

Q

I

L

K

E

I

L

E

R

K

D

G

G

active site: S78 (≠ T50), K192 (= K212), K194 (= K214), D223 (= D243), N225 (= N245), E249 (= E269), G274 (= G295), E275 (= E296), D298 (= D319), H325 (= H346), E345 (= E373)
binding (2r,3s,4r)-2,3,4-trihydroxy-5-(hydroxyamino)-5-oxopentanoic acid: K79 (≠ L51), F168 (≠ W188), K194 (= K214), E275 (= E296), H325 (= H346), E345 (= E373)
binding magnesium ion: D223 (= D243), E249 (= E269), E275 (= E296)

Sites not aligning to the query:

active site: 45
binding (2r,3s,4r)-2,3,4-trihydroxy-5-(hydroxyamino)-5-oxopentanoic acid: 43, 45

Q8ZL58 L-talarate/galactarate dehydratase; TalrD/GalrD; StTGD; EC 4.2.1.156; EC 4.2.1.42 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
25% identity, 88% coverage: 33:391/406 of query aligns to 64:366/398 of Q8ZL58

query
sites
Q8ZL58

V

I

T

A

N

E

L

I

T

R

N

S

E

R

N

D

G

G

I

F

T

E

G

G

T

V

G

G

L

F

A

S

F

Y

T

S

L
x
K

G
x
R

A

A

G

G

N

G

D

Q

L

G

V

I

C

Y

N

A

A

H

A

A

Q

K

F

E

Y

I

A

A

N

D

K

N

L

L

K

L

G

G

Q

E

D

D

I

P

E

N

E

D

L

I

M

D

S

K

D

I

F

Y

G

T

Q

K

T

L

F

L

R

-

T

-

L

-

S

-

N

-

E

-

Q

-

F

-

R

-

W

W

L

A

G

G

P

A

H

S

K

V

G

G

V

R

V

S

H

G

L

M

G

A

L

V

A

Q

S

A

V

I

T

S

N

P

-

I

-

D

-

I

A

A

C

L

Y

W

D

D

L

M

W

K

A

A

K

K

K

R

R

A

G

G

V

L

P

P

L

L

W

A

K

K

L

L

I

-

D

-

L

-

S

-

P

-

E

-

I

-

V

-

N

-

T

-

L

-

D

-

L

-

S

-

Y

-

L

-

E

-

D

-

V

-

L

-

T

-

K

-

E

-

A

-

I

-

A

-

M

-

L

-

Q

G

S

A

Q

H

T

R

D

D

S

S

K

V

K

Q

S

C

R

-

E

-

G

-

I

-

L

-

D

-

T

-

G

-

Y

-

P

-

G

-

Y

Y

D

N

T

T

S

S

V

G

G

G

W

F

F

L

N

H

Y

T

D

P

D

L

E

D

K

Q

V

V

R

L

E

K

N

N

C

V

K

V

K

I

A

S

I

R

A

E

N

N

G

G

F

I

T

G

A

G

M

I

K
|
K

L

L

K
|
K

V

V

G

G

S

Q

A

P

D

N

P

C

K

A

R

E

D

D

I

I

R

R

A

L

N

T

I

A

V

V

R

R

E

E

V

A

A

L

G

G

E

D

S

E

S

F

K

P

V

L

M

M

L

V

D
|
D

A

A

N
|
N

Q

Q

W

W

T

D

L

R

P

E

Q

T

A

A

I

I

S

R

I

M

C

G

N

R

E

K

L

M

K

E

N

Q

M

F

N

N

P

L

F

I

W

W

V

I

E
|
E

E

E

P

P

T

L

H

D

P

A

D

Y

D

D

V

I

L

E

A

G

H

H

Q

A

T

Q

L

L

A

A

R

A

E

A

I

L

A

-

P

D

V

T

K

P

L

I

A

A

L

T

G

G

E
|
E

H

M

V

L

P

T

N

S

R

F

I

R

I

E

F

H

K

E

N

Q

Y

L

L

I

Q

L

T

G

G

N

C

A

T

S

G

D

F

F

A

V

Q

Q

V

P

D

D

A

A

V

P

R

R

V

V

G

G

V

I

S

S

E

P

F

F

I

L

T

K

I

I

S

M

L

D

L

L

C

A

K

A

K

K

F

H

G

G

V

R

P

K

V

L

V

A

P

P

H
|
H

V

F

G

A

D

-

M

-

G

M

Q

E

L

V

H

H

Q

L

H

H

L

L

V

S

L

A

F

A

N

Y

H

P

I

L

A

E

M

P

G

-

H

-

E

-

A

-

L

-

F

W

L

L

E
|
E

H

H

I

F

P

E

H

W

L

L

K

N

Q

P

H

L

F

F

K

N

H

E

P

Q

I

L

K

E

I

L

E

R

K

D

G

G

KR 82:83 (≠ LG 51:52) binding
K195 (= K212) binding
K197 (= K214) active site, Proton acceptor; mutation to A: Loss of dehydration activity on both L-talarate and galactarate and loss of epimerization activity.
D226 (= D243) binding
N228 (= N245) binding
E252 (= E269) binding
E278 (= E296) binding
H328 (= H346) active site, Proton donor/acceptor; mutation H->N,A: Loss of dehydration activity on both L-talarate and galactarate and loss of epimerization activity.
E348 (= E373) binding

Sites not aligning to the query:

46:48 binding

2pp3A Crystal structure of l-talarate/galactarate dehydratase mutant k197a liganded with mg and l-glucarate (see paper)
25% identity, 88% coverage: 33:391/406 of query aligns to 61:363/395 of 2pp3A

query
sites
2pp3A

V

I

T

A

N

E

L

I

T

R

N

S

E

R

N

D

G

G

I

F

T

E

G

G

T

V

G

G

L

F

A

S

F

Y

T
x
S

L
x
K

G

R

A

A

G

G

N

G

D

Q

L

G

V

I

C

Y

N

A

A

H

A

A

Q

K

F

E

Y

I

A

A

N

D

K

N

L

L

K

L

G

G

Q

E

D

D

I

P

E

N

E

D

L

I

M

D

S

K

D

I

F

Y

G

T

Q

K

T

L

F

L

R

-

T

-

L

-

S

-

N

-

E

-

Q

-

F

-

R

-

W

W

L

A

G

G

P

A

H

S

K

V

G

G

V

R

V

S

H

G

L

M

G

A

L

V

A

Q

S

A

V

I

T

S

N

P

-

I

-

D

-

I

A

A

C

L

Y

W

D

D

L

M

W

K

A

A

K

K

K

R

R

A

G

G

V

L

P

P

L

L

W

A

K

K

L

L

I

-

D

-

L

-

S

-

P

-

E

-

I

-

V

-

N

-

T

-

L

-

D

-

L

-

S

-

Y

-

L

-

E

-

D

-

V

-

L

-

T

-

K

-

E

-

A

-

I

-

A

-

M

-

L

-

Q

G

S

A

Q

H

T

R

D

D

S

S

K

V

K

Q

S

C

R

-

E

-

G

-

I

-

L

-

D

-

T

-

G

-

Y

-

P

-

G

-

Y

Y

D

N

T

T

S

S

V

G

G

G

W

F

F

L

N

H

Y

T

D

P

D

L

E

D

K

Q

V

V

R

L

E

K

N

N

C

V

K

V

K

I

A

S

I

R

A

E

N

N

G

G

F

I

T

G

A

G

M

I

K
|
K

L

L

K
x
A

V

V

G

G

S

Q

A

P

D

N

P

C

K

A

R

E

D

D

I

I

R

R

A

L

N

T

I

A

V

V

R

R

E

E

V

A

A

L

G

G

E

D

S

E

S

F

K

P

V

L

M

M

L

V

D
|
D

A

A

N
|
N

Q

Q

W

W

T

D

L

R

P

E

Q

T

A

A

I

I

S

R

I

M

C

G

N

R

E

K

L

M

K

E

N

Q

M

F

N

N

P

L

F

I

W

W

V

I

E
|
E

E

E

P

P

T

L

H

D

P

A

D

Y

D

D

V

I

L

E

A

G

H

H

Q

A

T

Q

L

L

A

A

R

A

E

A

I

L

A

-

P

D

V

T

K

P

L

I

A

A

L

T

G
|
G

E
|
E

H

M

V

L

P

T

N

S

R

F

I

R

I

E

F

H

K

E

N

Q

Y

L

L

I

Q

L

T

G

G

N

C

A

T

S

G

D

F

F

A

V

Q

Q

V

P

D
|
D

A

A

V

P

R

R

V

V

G

G

V

I

S

S

E

P

F

F

I

L

T

K

I

I

S

M

L

D

L

L

C

A

K

A

K

K

F

H

G

G

V

R

P

K

V

L

V

A

P

P

H
|
H

V

F

G

A

D

-

M

-

G

M

Q

E

L

V

H

H

Q

L

H

H

L

L

V

S

L

A

F

A

N

Y

H

P

I

L

A

E

M

P

G

-

H

-

E

-

A

-

L

-

F

W

L

L

E
|
E

H

H

I
x
F

P

E

H

W

L

L

K

N

Q

P

H

L

F

F

K

N

H

E

P

Q

I

L

K

E

I

L

E

R

K

D

G

G

active site: S78 (≠ T50), K192 (= K212), A194 (≠ K214), D223 (= D243), N225 (= N245), E249 (= E269), G274 (= G295), E275 (= E296), D298 (= D319), H325 (= H346), E345 (= E373)
binding l-glucaric acid: K79 (≠ L51), K192 (= K212), D223 (= D243), N225 (= N245), E275 (= E296), H325 (= H346), E345 (= E373), F347 (≠ I375)
binding magnesium ion: D223 (= D243), E249 (= E269), E275 (= E296)

Sites not aligning to the query:

active site: 45
binding l-glucaric acid: 43

2og9A Crystal structure of mandelate racemase/muconate lactonizing enzyme from polaromonas sp. Js666
30% identity, 55% coverage: 182:406/406 of query aligns to 130:346/363 of 2og9A

query
sites
2og9A

Y

Y

D

N

T
|
T

S

S

V

G

G

G

W

F

F

L

N

H

Y

T

D

P

D

I

E

D

K

Q

V

L

R

M

E

V

N

N

C

A

K

S

K

A

A

S

I

I

A

E

N

R

G

G

F

I

T

G

A

G

M

I

K
|
K

L

L

K
|
K

V

V

G

G

S

Q

A

P

D

D

P

G

K

A

R

L

D

D

I

I

R

A

R

R

A

V

N

T

I

A

V

V

R

R

E

K

V

H

A

L

G

G

E

D

S

A

S

V

K

P

V

L

M

M

L

V

D
|
D

A

A

N
|
N

Q

Q

W

W

T

D

L

R

P

P

Q

T

A

A

I

Q

S

R

I

M

C

C

N

R

E

I

L

F

K

E

N

P

M

F

N

N

P

L

F

V

W

W

V

I

E
|
E

E

E

P

P

T

L

H

D

P

A

D

Y

D

D

V

H

L

E

A

G

H

H

Q

A

T

A

L

L

A
|
A

R

L

E

Q

I
x
F

A

D

P

-

V

T

K

P

L

I

A

A

L

T

G
|
G

E
|
E

H

M

V

L

P

T

N

S

R

A

I

A

I

E

F

H

K

G

N

D

Y

L

L

I

Q

R

T

H

G

R

C

A

T

A

G

D

F

Y

A

L

Q
x
M

V

P

D
|
D

A

A

V

P

R

R

V

V

G

G

V

I

S

T

E

P

F

F

I

L

T

K

I

I

S

A

L

S

L

L

C

A

K

E

K

H

F

A

G

G

V

L

P

M

V

L

V

A

P

P

H
|
H

V
x
F

G
x
A

D

M

M

-

G

-

Q

E

L

L

H

H

Q

V

H

H

L

L

V

A

L

-

F

-

N

-

H

-

I

-

A

A

M

A

G

Y

H

P

E

R

A

E

L

P

F

W

L

V

E
|
E

H

H

I

F

P

E

H

W

L

L

K

E

Q

P

H

L

F

F

K

N

H

E

P

R

I

I

K

E

I

I

E

R

K

D

G

G

V

R

Y

M

I

L

T

V

P

P

Q

T

E

R

A

P

G

G

S

L

S

G

C

L

D

T

L

L

K

S

active site: T132 (= T184), K160 (= K212), K162 (= K214), D191 (= D243), N193 (= N245), E217 (= E269), G242 (= G295), E243 (= E296), M264 (≠ Q317), D266 (= D319), H293 (= H346), F294 (≠ V347), A295 (≠ G348), E313 (= E373)
binding calcium ion: A232 (= A284), F235 (≠ I287)

Sites not aligning to the query:

active site: 46

3cb3A Crystal structure of l-talarate dehydratase from polaromonas sp. Js666 complexed with mg and l-glucarate
31% identity, 54% coverage: 182:400/406 of query aligns to 141:351/373 of 3cb3A

query
sites
3cb3A

Y

Y

D

N

T
|
T

S

S

V

G

G

G

W

F

F

L

N

H

Y

T

D

P

D

I

E

D

K

Q

V

L

R

M

E

V

N

N

C

A

K

S

K

A

A

S

I

I

A

E

N

R

G

G

F

I

T

G

A

G

M

I

K
|
K

L

L

K
|
K

V

V

G

G

S

Q

A

P

D

D

P

G

K

A

R

L

D

D

I

I

R

A

R

R

A

V

N

T

I

A

V

V

R

R

E

K

V

H

A

L

G

G

E

D

S

A

S

V

K

P

V

L

M

M

L

V

D
|
D

A

A

N
|
N

Q

Q

W

W

T

D

L

R

P

P

Q

T

A

A

I

Q

S

R

I

M

C

C

N

R

E

I

L

F

K

E

N

P

M

F

N

N

P

L

F

V

W

W

V

I

E
|
E

E

E

P

P

T

L

H

D

P

A

D

Y

D

D

V

H

L

E

A

G

H

H

Q

A

T

A

L

L

A
|
A

R

L

E

Q

I
x
F

A

-

P

D

V

T

K

P

L

I

A

A

L

T

G
|
G

E
|
E

H

M

V

L

P

T

N

S

R

A

I

A

I

E

F

H

K

G

N

D

Y

L

L

I

Q

R

T

H

G

R

C

A

T

A

G

D

F

Y

A

L

Q
x
M

V

P

D
|
D

A

A

V

P

R

R

V

V

G

G

V

I

S

T

E

P

F

F

I

L

T

K

I

I

S

A

L

S

L

L

C

A

K

E

K

H

F

A

G

G

V

L

P

M

V

L

V

A

P

P

H
|
H

V
x
F

G
x
A

D

M

M

-

G

-

Q

E

L

L

H

H

Q

V

H

H

L

L

V

A

L

-

F

-

N

-

H

-

I

-

A

A

M

A

G

Y

H

P

E

R

A

E

L

P

F

W

L

V

E
|
E

H

H

I

F

P

E

H

W

L

L

K

E

Q

P

H

L

F

F

K

N

H

E

P

R

I

I

K

E

I

I

E

R

K

D

G

G

V

R

Y

M

I

L

T

V

P

P

Q

T

E

R

A

P

G

G

active site: T143 (= T184), K171 (= K212), K173 (= K214), D202 (= D243), N204 (= N245), E228 (= E269), G253 (= G295), E254 (= E296), M275 (≠ Q317), D277 (= D319), H304 (= H346), F305 (≠ V347), A306 (≠ G348), E324 (= E373)
binding l-glucaric acid: K171 (= K212), K173 (= K214), D202 (= D243), E254 (= E296), H304 (= H346)
binding magnesium ion: D202 (= D243), E228 (= E269), A243 (= A284), F246 (≠ I287), E254 (= E296)

Sites not aligning to the query:

active site: 24, 57

5xd8B Crystal structure analysis of 3,6-anhydro-l-galactonate cycloisomerase (see paper)
27% identity, 63% coverage: 102:358/406 of query aligns to 99:310/367 of 5xd8B

query
sites
5xd8B

G

G

V

I

V

S

H

T

L

F

G

A

L

M

A

S

S

A

V

V

T

D

N

I

A

A

C

L

Y

W

D

D

L

L

W

K

A

G

K

K

K

R

R

E

G

G

V

L

P

P

L

L

W

W

K

K

L

-

I

-

D

-

L

-

S

-

P

-

E

-

I

-

V

-

N

-

T

-

L

-

D

-

L

-

S

-

Y

-

L

-

E

-

D

-

V

-

L

-

T

-

K

-

E

-

A

-

I

-

A

-

M

M

L

A

Q

G

S

G

Q

K

T

N

D

N

S

T

K

C

K

K

S

A

R

Y

E

C

G
|
G

I

G

L

I

D

D

T

L

G

Q

Y

F

P

P

G

L

Y

-

D

-

T

-

S

-

V

-

G

-

W

-

F

-

N

-

Y

-

D

-

E

E

K

K

V

L

R

L

E

N

N

N

C

I

K

C

K

G

A

Y

I

L

A

E

N

S

G

G

F

F

T

N

A

A

M

V

K
|
K

L

I

K
|
K

V

I

G

G

S

R

A

E

D

N

P

M

K

Q

R

E

D

D

I

I

R

D

R

R

A

I

N

K

I

A

V

V

R

R

E

E

V

L

A

I

G

G

E

P

S

D

S

I

K

T

V

F

M

M

L

I

D
|
D

A

A

N
|
N

Q

Y

Q

S

W

L

T

T

L

V

P

E

Q

Q

A

A

I

I

S

K

I

L

C

S

N

K

E

A

L

V

K

E

N

Q

M

Y

N

D

P

I

F

T

W

W

V

F

E
|
E

E

E

P

P

T

T

H

L

P

P

D

D

V

Y

L

K

A

G

H

F

Q

A

T

E

L

I

A

A

R

D

E

N

I

T

A

A

P

-

V

I

K

P

L

L

A

A

L

M

G
|
G

E
|
E

H

N

V

L

P

H

N

T

R

I

I

H

I

E

F

F

K

G

N

Y

Y

A

L

M

Q

D

T

Q

G

A

C

K

T

L

G

G

F

Y

A

C

Q
|
Q

V

P

D
|
D

A

A

V

S

R

N

V

C

G

G

V

I

S

T

E

G

F

W

I

L

T

K

I

A

S

A

L

D

L

L

C

I

K

T

K

E

F

H

G

N

V

I

P

P

V

V

V

C

P

T

H
|
H

V

-

G

-

D
x
G

M
|
M

G

Q

Q

E

L

L

H

H

Q

V

H

S

L

L

V

V

active site: G140 (= G173), K167 (= K212), K169 (= K214), D198 (= D243), N200 (= N245), E224 (= E269), G249 (= G295), E250 (= E296), Q271 (= Q317), D273 (= D319), H300 (= H346), G301 (≠ D349), M302 (= M350)
binding magnesium ion: D198 (= D243), E224 (= E269), E250 (= E296)

Sites not aligning to the query:

active site: 22, 53, 317, 319, 324

Q9RKF7 3,6-anhydro-alpha-L-galactonate cycloisomerase; AHGA cycloisomerase; EC 5.5.1.25 from Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
23% identity, 100% coverage: 1:405/406 of query aligns to 1:350/361 of Q9RKF7

query
sites
Q9RKF7

M

M

I

I

N

E

K

R

I

V

E

R

I

T

S

D

D

L

K

Y

R

R

F

I

E

P

L

L

S

P

T

T

G

R

A

L

G

-

S

T

D

D

A

S

I

T

H

H

K

G

D

A

P

M

Q

M

-

D

Y

F

S

E

Y

L

A

I

V

T

T

V

N

R

L

I

T

E

N

D

E

S

N

D

G

G

I

A

T

T

G

G

T

L

G

G

L

Y

A

T

F

Y

T

T

L

V

G

N

A

H

G

G

N

G

D

A

L

A

V

V

C

A

N

T

A

M

A

V

-

D

Q

K

F

D

Y

L

A

R

N

G

K

C

L

L

K

L

G

G

Q

A

D

D

I

A

E

E

E

Q

L

I

M

E

S

-

D

-

F

-

G

-

Q

K

T

I

F

W

R

Q

T

S

L

M

S

W

N

W

E

R

Q

L

Q

H

F

Y

R

A

W

G

L

R

G

G

P

G

H

H

K

A

G

T

V

S

V

A

H

-

L

-

G

-

L

I

A

S

S

A

V

V

T

D

N

I

A

A

C

L

Y

W

D

D

L

L

W

K

A

G

K

I

K

R

R

A

G

R

V

T

P

P

L

L

W

W

K

K

L

L

L

F

I

G

D

G

L

Y

S

D

P

P

E

V

I

P

V

V

-

Y

-

A

N

G

T

G

L

I

D

D

L

L

S

-

Y

-

L

-

E

-

D

-

V

-

L

-

T

-

K

-

E

E

E

L

A

P

I

V

A

A

M

D

L

L

Q

K

S

T

Q

Q

T

A

D

D

S

-

K

-

S

-

R

-

E

-

G

-

I

-

L

-

D

-

T

-

G

-

Y

-

P

-

G

-

Y

-

D

-

T

-

S

-

V

-

G

-

W

-

F

-

N

-

Y

-

D

-

E

-

K

-

V

-

R

-

E

-

N

-

C

-

K

-

K

R

A

F

I

L

A

A

N

G

G

G

F

F

T

R

A

A

M

I

K

K

L

M

K

K

V

V

G

G

S

R

A

P

D

D

P

L

K

K

R

E

D

D

I

V

R

D

R

R

A

V

N

S

I

A

V

L

R

R

E

E

V

H

A

L

G

G

E

D

S

S

S

F

K

P

V

L

M

M

L

V

D
|
D

A

A

N

N

Q

M

Q

K

W

W

T

T

L

V

P

D

Q

G

A

A

I

I

S

R

I

A

C

A

N

R

E

A

L

L

K

A

N

P

M

F

N

D

P

L

F

H

W

W

V

I

E
|
E

E

E

P

P

T

T

H

I

P

P

D

D

V

L

L

V

A

G

H

N

Q

A

T

R

L

I

A

V

R

R

E

E

I

S

A

G

P

H

V

T

K

-

L

I

A

A

L

G

G

G

E
|
E

H

N

V

L

P

H

N

T

R

L

I

Y

I

D

F

F

K

H

N

N

Y

A

L

V

Q

R

T

A

G

G

C

S

T

L

G

T

F

L

A

P

Q

E

V

P

D

D

A

V

V

S

R

N

V

I

G

G

V

Y

S

T

E

T

F

F

I

R

T

K

I

V

S

A

L

A

L

L

C

A

K

E

K

A

F

N

G

N

V

M

P

L

V

L

V

T

P

S

H

H

V

-

G

-

D

-

M

-

G

-

Q

G

L

V

H

H

Q

D

H

-

L

L

V

T

L

V

F

H

N

A

H

L

I

A

A

S

M

V

G

P

H

H

E

R

A

T

L

Y

F

M

L

E

E

A

H

H

I

G

P

F

H

G

L

L

K

H

Q

A

H

Y

F

M

K

A

H

E

P

P

I

M

K

A

I

V

E

T

K

D

G

G

V

C

Y

V

I

S

T

A

P

P

Q

D

E

R

A

P

G

G

S

H

S

G

C

V

D

V

L

L

D195 (= D243) binding
E221 (= E269) binding
E247 (= E296) binding

3ck5A Crystal structure of a racemase from streptomyces coelicolor a3(2) with bound magnesium
23% identity, 99% coverage: 1:400/406 of query aligns to 1:342/357 of 3ck5A

query
sites
3ck5A

M

L

I

I

N

E

K

R

I

V

E

R

I

T

S

D

D

L

K

Y

R

R

F

I

E

P

L

L

S

P

T

T

G

R

A

L

G

-

S
x
T

D

D

A

S

I

T

H

H

K

G

D

A

P

M

Q

M

-

D

Y

F

S

E

Y

L

A

I

V

T

T

V

N

R

L

I

T

E

N

D

E

S

N

D

G

G

I

A

T

T

G

G

T

L

G

G

L

Y

A

T

F

Y

T
|
T

L

V

G

N

A

H

G

G

N

G

D

A

L

A

V

V

C

A

N

T

A

M

A

V

-

D

Q

K

F

D

Y

L

A

R

N

G

K

C

L

L

K

L

G

G

Q

A

D

D

I

A

E

E

E

Q

L

I

M

E

S

-

D

-

F

-

G

-

Q

K

T

I

F

W

R

Q

T

S

L

M

S

W

N

W

E

R

Q

L

Q

H

F

Y

R

A

W

G

L

R

G

G

P

G

H

H

K

A

G

T

V

S

V

-

H

-

L

-

G

A

L

I

A

S

S

A

V

V

T

D

N

I

A

A

C

L

Y

W

D

D

L

L

W

K

A

G

K

I

K

R

R

A

G

R

V

T

P

P

L

L

W

W

K

K

L

L

L

F

I

G

D

G

L

Y

S

D

P

P

E

V

I

P

V

V

-

Y

-

A

N
x
G

T

G

L

I

D

D

L

L

S

-

Y

-

L

-

E

-

D

-

V

-

L

-

T

-

K

-

E

E

E

L

A

P

I

V

A

A

M

D

L

L

Q

K

S

T

Q

Q

T

A

D

D

S

-

K

-

S

-

R

-

E

-

G

-

I

-

L

-

D

-

T

-

G

-

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active site: T19 (≠ S20), T50 (= T50), G137 (≠ N141), K164 (= K212), K166 (= K214), D195 (= D243), N197 (= N245), I220 (≠ V268), E221 (= E269), I243 (≠ L292), G246 (= G295), E247 (= E296), E268 (≠ Q317), D270 (= D319), H297 (= H346), G298 (≠ H354), V299 (≠ Q355), Y315 (≠ F371), E317 (= E373)
binding magnesium ion: D195 (= D243), E221 (= E269), E247 (= E296)

3op2A Crystal structure of putative mandelate racemase from bordetella bronchiseptica rb50 complexed with 2-oxoglutarate/phosphate
30% identity, 55% coverage: 178:400/406 of query aligns to 132:354/375 of 3op2A

query
sites
3op2A

G

G

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active site: S138 (≠ T184), K165 (= K212), K167 (= K214), D195 (= D243), N197 (= N245), E221 (= E269), G247 (= G295), E248 (= E296), N249 (≠ H297), Q269 (= Q317), D271 (= D319), H298 (= H346), T299 (≠ V347), F300 (vs. gap), E323 (≠ L372), I326 (= I375), H328 (= H377)
binding 2-oxoglutaric acid: K165 (= K212), K167 (= K214), D195 (= D243), E248 (= E296), H298 (= H346), E323 (≠ L372)
binding magnesium ion: D195 (= D243), E221 (= E269), E248 (= E296)

Sites not aligning to the query:

active site: 20, 53, 56

3ozmD Crystal structure of enolase superfamily member from bordetella bronchiseptica complexed with mg, m-xylarate and l-lyxarate
30% identity, 55% coverage: 178:400/406 of query aligns to 137:359/381 of 3ozmD

query
sites
3ozmD

G

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active site: S143 (≠ T184), K170 (= K212), K172 (= K214), D200 (= D243), N202 (= N245), E226 (= E269), G252 (= G295), E253 (= E296), N254 (≠ H297), Q274 (= Q317), D276 (= D319), H303 (= H346), T304 (≠ V347), F305 (vs. gap), E328 (≠ L372), I331 (= I375), H333 (= H377)
binding L-arabinaric acid: K172 (= K214), D200 (= D243), N202 (= N245), E253 (= E296), H303 (= H346), F305 (vs. gap), E328 (≠ L372)
binding magnesium ion: D200 (= D243), E226 (= E269), E253 (= E296)

Sites not aligning to the query:

active site: 20, 53, 56

3ozmA Crystal structure of enolase superfamily member from bordetella bronchiseptica complexed with mg, m-xylarate and l-lyxarate
30% identity, 55% coverage: 178:400/406 of query aligns to 137:359/386 of 3ozmA

query
sites
3ozmA

G

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active site: S143 (≠ T184), K170 (= K212), K172 (= K214), D200 (= D243), N202 (= N245), E226 (= E269), G252 (= G295), E253 (= E296), N254 (≠ H297), Q274 (= Q317), D276 (= D319), H303 (= H346), T304 (≠ V347), F305 (vs. gap), E328 (≠ L372), I331 (= I375), H333 (= H377)
binding D-xylaric acid: Y146 (≠ G187), K170 (= K212), K172 (= K214), D200 (= D243), N202 (= N245), E253 (= E296), H303 (= H346), F305 (vs. gap), E328 (≠ L372)
binding magnesium ion: D200 (= D243), E226 (= E269), E253 (= E296)

Sites not aligning to the query:

active site: 20, 53, 56
binding D-xylaric acid: 24, 29

Query Sequence

>CA265_RS04225 FitnessBrowser__Pedo557:CA265_RS04225
MINKIEISDKRFELSTGAGSDAIHKDPQYSYAVTNLTNENGITGTGLAFTLGAGNDLVCN
AAQFYANKLKGQDIEELMSDFGQTFRTLSNEQQFRWLGPHKGVVHLGLASVTNACYDLWA
KKRGVPLWKLLIDLSPEEIVNTLDLSYLEDVLTKEEAIAMLQSQTDSKKSREGILDTGYP
GYDTSVGWFNYDDEKVRENCKKAIANGFTAMKLKVGSADPKRDIRRANIVREVAGESSKV
MLDANQQWTLPQAISICNELKNMNPFWVEEPTHPDDVLAHQTLAREIAPVKLALGEHVPN
RIIFKNYLQTGCTGFAQVDAVRVGGVSEFITISLLCKKFGVPVVPHVGDMGQLHQHLVLF
NHIAMGHEALFLEHIPHLKQHFKHPIKIEKGVYITPQEAGSSCDLK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory