SitesBLAST

P9WP51 Probable cystathionine beta-synthase Rv1077; Beta-thionase; Serine sulfhydrase; EC 4.2.1.22 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
41% identity, 99% coverage: 4:452/453 of query aligns to 5:458/464 of P9WP51

query
sites
P9WP51

N

Q

N

H

I

I

L

S

E

E

T

L

I

I

G

G

N

G

T

T

P

P

L

L

V

V

K

R

L

L

N

N

T

S

I

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T

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P

G

D

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G

P

A

G

G

T

T

I

V

L

A

A

A

K

K

I

V

E

E

T

Y

T

L

N

N

P

P

G

G

N

G

S

S

I

S

K

K

D

D

R

R

M

I

A

A

V

V

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K

M

M

I

I

E

E

D

A

A

A

E

E

K

A

S

S

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K

Q

L

L

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K

P

P

G

G

T

T

I

I

I

V

E

E

G

P

T

T

S

S

G

G

N

N

T

T

G

G

M

V

G

G

L

L

A

A

M

L

A

V

A

A

I

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I

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Y

Y

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C

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D

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E

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N

A

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I

A

A

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Y

G

G

A

A

E

E

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I

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C

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A

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D

N

G

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A

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K

P

P

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Y

Y

D

A

N

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L

P

A

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Y

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D

-

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Y

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E

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G

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A

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N

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M

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x
K

L

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Y

D

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N

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A

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S

K428 (≠ S421) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

7qgtB Crystal structure of human cystathionine beta-synthase (delta516-525) in complex with aoaa. (see paper)
37% identity, 99% coverage: 5:453/453 of query aligns to 39:495/500 of 7qgtB

query
sites
7qgtB

N

D

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I

L

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E

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T

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I

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G

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N

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T

K

I

I

T

G

K

K

-

K

-

F

G

G

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L

P

K

G

C

T

E

I

L

L

L

A

A

K

K

I

C

E

E

T

F

N

N

P

A

G

G

N

G

S

S

I

V

K
|
K

D

D

R

R

M

I

A

S

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L

K

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M

M

I

I

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D

D

A

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G

G

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L

L

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K

P

P

G

G

G

D

T

T

I

I

E

E

G

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T
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T

S
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S

G

G

N
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N

T
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T

G

G

M

I

G

G

L

L

A

A

M

L

A

A

I

A

I

V

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R

G

G

Y

Y

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K

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A

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L

G

G

A

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C

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P

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N

N

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Y

H

Y

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N

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P

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W

H

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P

L

N

D

Q
|
Q

Y

Y

D

R

N

N

L
x
A

A

S

N

N

S
x
P

Q
x
L

A

A

H

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Y
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Y

E

D

Q

T

T

T

G

A

P

D

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E

I

I

W

L

A

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K

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M

L

L

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G

T
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T

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G

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T

I

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T

G

G

T

I

G

A

K
x
R

Y

K

L

L

K

K

E

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K

K

N

C

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A

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G

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Y

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Y

Y

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T

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G
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G

I

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G

G

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Y

D

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A

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N

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S

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S

H

L

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M

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N

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F

A

A

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Q

I

M

-

V

-

F

E

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A

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D

D

I

L

N

N

A

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A

G

A

binding protoporphyrin ix containing fe: A186 (≠ L150), P189 (≠ S153), L190 (≠ Q154), Y193 (= Y157), R226 (≠ K190)
binding 4'-deoxy-4'-acetylyamino-pyridoxal-5'-phosphate: K79 (= K43), T106 (= T70), S107 (= S71), N109 (= N73), T110 (= T74), Q182 (= Q146), G216 (= G180), T217 (= T181), G218 (= G182), T220 (= T184), G265 (= G232), S309 (= S276), P335 (≠ H302), D336 (= D303)

Sites not aligning to the query:

binding protoporphyrin ix containing fe: 10, 11, 12, 13, 14, 23, 24, 25

8s5hA Full-length human cystathionine beta-synthase with c-terminal 6xhis- tag, basal state, helical reconstruction (see paper)
37% identity, 99% coverage: 5:453/453 of query aligns to 38:504/507 of 8s5hA

query
sites
8s5hA

N

D

I

I

L

L

E

K

T

K

I

I

G

G

N

D

T

T

P

P

L

M

V

V

K

R

L

I

N

N

T

K

I

I

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G

K

K

-

K

-

F

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G

C

T

E

I

L

L

L

A

A

K

K

I

C

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F

N

N

P

A

G

G

N

G

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S

I

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K

D

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M

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I

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S

S

G

G

N

N

T

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G

G

M

I

G

G

L

L

A

A

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L

A

A

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A

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G

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C

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K

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D

A

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L

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A

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L

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G

A

A

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I

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N

N

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N

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P

N

N

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S

W

H

K

I

P

L

N

D

Q

Q

Y

Y

D

R

N

N

L
x
A

A

S

N

N

S

P

Q

L

A

A

H

H

Y
|
Y

E

D

Q

T

T

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G

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G

G

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G

G

T

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G

A

K
x
R

Y

K

L

L

K

K

E

E

K

K

N

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A

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G

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F

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D

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G

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S

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-

I

-

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Y

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-

V

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Y

I

D

D

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N

N

A

F

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A

G

A

binding protoporphyrin ix containing fe: A185 (≠ L150), Y192 (= Y157), R225 (≠ K190)

Sites not aligning to the query:

binding protoporphyrin ix containing fe: 10, 11, 17, 22, 23, 24

P35520 Cystathionine beta-synthase; Beta-thionase; Serine sulfhydrase; EC 4.2.1.22 from Homo sapiens (Human) (see 40 papers)
37% identity, 99% coverage: 5:453/453 of query aligns to 79:545/551 of P35520

query
sites
P35520

N

D

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G

N

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N

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T

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G

K

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-

K

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F

G

G

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x
L

P
x
K

G

C

T

E

I

L

L

L

A

A

K

K

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x
C

E

E

T

F

N

N

P
x
A

G

G

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G

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K

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M

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A

G85 (= G11) to R: in CBSD; loss of cystathionine beta-synthase activity; dbSNP:rs863223435
T87 (= T13) to N: in CBSD; decreased cystathionine beta-synthase activity; increased aggregation
L101 (≠ V25) to P: in CBSD; common mutation in Irish population; loss of activity; dbSNP:rs786204757
K102 (≠ P26) to N: in CBSD; associated in cis with R-78; decreased cystathionine beta-synthase activity; decreased homotetramer formation; dbSNP:rs786204609; to Q: in dbSNP:rs34040148
C109 (≠ I33) to R: in CBSD; loss of activity; dbSNP:rs778220779
A114 (≠ P38) to V: in CBSD; mild form; when linked with W-58 severe form; decreased cystathionine beta-synthase activity; decreases homotetramer formation by promoting formation of larger aggregates; dbSNP:rs121964964
K119 (= K43) modified: N6-(pyridoxal phosphate)lysine
R125 (≠ K49) to Q: in CBSD; severe form; when linked with D-131 moderate form; loss of cystathionine beta-synthase activity; decreased homotetramer formation; dbSNP:rs781444670; to W: in CBSD; exhibits an activity lower than 4% of the wild-type enzyme; absent capacity to form multimeric quaternary structure; dbSNP:rs886057100
M126 (= M50) to V: in CBSD; loss of activity
E131 (= E55) to D: in CBSD; linked with Q-125; loss of activity; dbSNP:rs1555875351
G139 (= G63) to R: in CBSD; mild form; dbSNP:rs121964965
I143 (= I67) to M: in CBSD; 4% of activity; stable; dbSNP:rs370167302
E144 (= E68) to K: in CBSD; loss of cystathionine beta-synthase activity; impaired stimulation by AdoMet and AdoHcy; decreased homotetramer formation; dbSNP:rs121964966
G148 (= G72) to R: in CBSD; loss of cystathionine beta-synthase activity; impaired stimulation by AdoMet and AdoHcy; loss of homotetramer formation; dbSNP:rs755952006
N149 (= N73) binding pyridoxal 5'-phosphate
L154 (= L78) to Q: in CBSD; protein expression is comparable to wild-type; significant decrease of enzyme activity
A155 (= A79) to T: in CBSD; complete loss of activity; severely affects homotetramer formation by promoting formation of larger aggregates; dbSNP:rs1429138569; to V: in CBSD; protein expression is comparable to wild-type; significant decrease of enzyme activity
C165 (= C89) to Y: in CBSD; severe form; protein expression is comparable to wild-type; loss of cystathionine beta-synthase activity; no effect on homotetramer formation; dbSNP:rs1347651454
M173 (≠ Q97) to V: in CBSD; presents 40% of the wild-type activity; highly reduced capacity to form multimeric quaternary structure; natural variant: Missing (in CBSD; loss of activity)
E176 (= E100) to K: in CBSD; severe form; loss of cystathionine beta-synthase activity; inhibited by AdoMet; severely decreases homotetramer formation by promoting formation of larger aggregates; dbSNP:rs762065361
V180 (≠ A104) to A: in CBSD; decreased cystathionine beta-synthase activity; decreases homotetramer formation; dbSNP:rs1555875010
T191 (≠ C115) to M: in CBSD; moderate and severe forms; loss of cystathionine beta-synthase activity; absent capacity to form multimeric quaternary structure; dbSNP:rs121964973
K211 (≠ E135) modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)
A226 (≠ L150) to T: in CBSD; presents 20% of the wild-type activity; dramatically reduced capacity to form multimeric quaternary structure; dbSNP:rs763835246
N228 (= N152) to K: in CBSD; loss of cystathionine beta-synthase activity; decreased homotetramer formation; dbSNP:rs1464223176; to S: in CBSD; has significantly decreased levels of enzyme activity; dbSNP:rs1555874803
A231 (= A155) to P: in CBSD; has significantly decreased levels of enzyme activity
D234 (≠ E158) to N: in CBSD; decreased cystathionine beta-synthase activity; changed localization; decreased interaction with pyridoxal 5'-phosphate; no effect on homotetramer formation; dbSNP:rs773734233; natural variant: Missing (in CBSD; protein expression is comparable to wild-type; significant decrease of enzyme activity)
GTGGT 256:260 (= GTGGT 180:184) binding pyridoxal 5'-phosphate
T257 (= T181) to M: in CBSD; moderate to severe form; protein expression is comparable to wild-type; significant decrease of enzyme activity; dbSNP:rs758236584
T262 (≠ S186) to M: in CBSD; moderate form; dbSNP:rs149119723; to R: in CBSD; severe form; loss of cystathionine beta-synthase activity; loss of homotetramer formation
R266 (≠ K190) to K: in CBSD; mild form; decreased cystathionine beta-synthase activity; decreased homotetramer formation; no effect on heme-binding; decreased stability; dbSNP:rs121964969
K269 (= K193) natural variant: Missing (in CBSD; loss of expression)
C272 (≠ N196) mutation to A: Reduced heme content and cystathionine beta-synthase activity.
C275 (≠ I199) to Y: in CBSD; severe form; exhibits an activity lower than 4% of the wild-type enzyme; absent capacity to form multimeric quaternary structure; mutation to S: Reduced heme content and cystathionine beta-synthase activity.
I278 (≠ W202) to S: in CBSD; loss of activity; to T: in CBSD; mild to severe form; common mutation; decreased expression; loss of cystathionine beta-synthase activity; impaired stimulation by AdoMet and AdoHcy; severely affects homotetramer formation by promoting formation of larger aggregates; dbSNP:rs5742905
D281 (= D205) to N: in CBSD; loss of activity
A288 (≠ K212) to T: in CBSD; protein expression is comparable to wild-type; significant decrease of enzyme activity; dbSNP:rs141502207
E302 (≠ I229) to K: in CBSD; no effect on cystathionine beta-synthase activity; inhibited by AdoHcy and impaired activation by AdoMet; no effect on homotetramer formation; dbSNP:rs779270933
G305 (= G232) to R: in CBSD; loss of cystathionine beta-synthase activity; no effect on homotetramer formation
G307 (= G234) to S: in CBSD; moderate to severe form; linked with D-534; common mutation; loss of cystathionine beta-synthase activity; impaired stimulation by AdoMet and AdoHcy; no effect on homotetramer formation; dbSNP:rs121964962
V320 (≠ I247) to A: in CBSD; has 36% of wild-type enzyme activity; dbSNP:rs781567152
D321 (= D248) to V: in CBSD; loss of activity
R336 (= R263) to C: in CBSD; protein expression is comparable to wild-type; loss of activity; absent capacity to form multimeric quaternary structure; dbSNP:rs398123151; to H: in CBSD; mild form; no effect on expression; exhibits an activity lower than 4% of the wild-type enzyme; altered stimulation by AdoMet; absent capacity to form multimeric quaternary structure; dbSNP:rs760417941
L338 (≠ I265) to P: in CBSD; severe form; exhibits an activity lower than 4% of the wild-type enzyme; absent capacity to form multimeric quaternary structure
G347 (= G274) to S: in CBSD; protein expression is comparable to wild-type; loss of activity; dbSNP:rs771298943
S349 (= S276) binding pyridoxal 5'-phosphate; to N: in CBSD; severe form; exhibits an activity lower than 4% of the wild-type enzyme; absent capacity to form multimeric quaternary structure
T353 (≠ A280) to M: in CBSD; protein expression is comparable to wild-type; significant decrease of enzyme activity; dbSNP:rs121964972
R369 (≠ V296) to C: in CBSD; when linked with C-491 severe form; decreased cystathionine beta-synthase activity; decreased homotetramer formation; dbSNP:rs117687681
D376 (= D303) to N: in CBSD; has significantly decreased levels of enzyme activity; dbSNP:rs1170128038
R379 (≠ S306) to Q: in CBSD; exhibits an activity lower than 4% of the wild-type enzyme; absent capacity to form multimeric quaternary structure; dbSNP:rs763036586
K384 (= K311) to E: in CBSD; severe form; dbSNP:rs121964967
P422 (≠ Q349) to L: in CBSD; changed cystathionine beta-synthase activity; impaired stimulation by AdoMet; does not affect homotetramer formation; dbSNP:rs28934892
P427 (≠ E354) to L: in CBSD; no effect on cystathionine beta-synthase activity; altered stimulation by AdoMet; dbSNP:rs863223434
I435 (vs. gap) to T: in CBSD; no effect on cystathionine beta-synthase activity; impaired stimulation by AdoMet and AdoHcy; does not affect homotetramer formation
R439 (≠ K360) to Q: in CBSD; no effect on cystathionine beta-synthase activity; increased homotetramer formation; dbSNP:rs756467921
D444 (≠ S365) to N: in CBSD; decreased expression; no effect on cystathionine beta-synthase activity; altered stimulation by AdoMet; increased homotetramer formation; dbSNP:rs28934891
V449 (≠ T370) to G: in CBSD; no effect on cystathionine beta-synthase activity; altered stimulation by AdoMet
L456 (≠ V376) to P: in CBSD; severe; exhibits an activity lower than 4% of the wild-type enzyme; absent capacity to form multimeric quaternary structure
S466 (= S386) to L: in CBSD; increased cystathionine beta-synthase activity; impaired stimulation by AdoMet and AdoHcy; decreased homotetramer formation; dbSNP:rs121964971
S500 (= S420) to L: in CBSD; no effect on cystathionine beta-synthase activity; altered stimulation by AdoMet; dbSNP:rs755106884
Q526 (vs. gap) to K: in CBSD; has significantly decreased levels of enzyme activity
L539 (≠ I447) to S: in CBSD; loss of cystathionine beta-synthase activity; impaired stimulation by AdoMet and AdoHcy; loss of homotetramer formation; dbSNP:rs121964968
L540 (≠ I448) to Q: in CBSD; no effect on cystathionine beta-synthase activity; altered stimulation by AdoMet

Sites not aligning to the query:

18 R → C: results in 1/3 to 2/3 the enzyme activity of the wild-type; dbSNP:rs201827340
49 P → L: in CBSD; decreased expression; no effect on cystathionine beta-synthase activity; increased homotetramer formation; dbSNP:rs148865119
52 binding axial binding residue
58 R → W: in CBSD; linked with V-114; 18% of activity; dbSNP:rs555959266
65 binding axial binding residue; H → R: in CBSD; decreased cystathionine beta-synthase activity; inhibited by AdoMet and AdoHcy; decreased homotetramer formation; dbSNP:rs1191141364
69 A → P: in dbSNP:rs17849313
78 P → R: in CBSD; severe form; associated in cis with N-102; decreased cystathionine beta-synthase activity; decreased homotetramer formation; dbSNP:rs786204608
548 R → Q: presents 60% of the wild-type activity; highly reduced capacity to form multimeric quaternary structure; dbSNP:rs150828989

4pcuA Crystal structure of delta516-525 e201s human cystathionine beta- synthase with adomet (see paper)
36% identity, 99% coverage: 5:453/453 of query aligns to 37:486/486 of 4pcuA

query
sites
4pcuA

N

D

I

I

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L

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K

T

K

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G

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K

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K

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G

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A

A

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N

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Y

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-

G

-

I

-

F

L

D

N

K

Q

D

T

E

E

I

Q

Y

T

P

T

Y

Y

I

E

T

V

E

E

G
|
G

I

I

G

G

E

Y

D

D

F

F

L

I

P

P

A
x
T

N

V

V

L

N

D

F

R

D

T

V

V

I

V

D

D

L

K

F

W

E

F

K

K

V

S

T

N

D

D

K

E

D

E

A

A

A

F

L

T

M

F

T

A

R

R

D

M

I

L

A

I

R

A

K

Q

E

E

G

G

I

L

F

L

V

C

G

G

N

G

S
|
S

A

A

G

G

A

S

A

T

I

V

G

A

G

V

L

A

I

V

Q

K

L

A

K

A

D

Q

K

E

L

L

K

Q

P

E

E

G

D

Q

V

R

V

C

V

V

I

I

F

L

H
x
P

D
|
D

H

S

G

V

S

R

R

N

Y

Y

M

M

G

T

K

K

M

F

Y

L

N

S

E

D

D

R

W

W

L

M

R

L

E

Q

R

K

G

G

F

F

L

L

Q

K

D

E

E

E

K

D

L

L

T

-

A

-

K

K

S

K

I

P

L

W

S

W

K

W

K

H

E

L

S

R

T

V

E

Q

I

E

V

L

T

G

L

L

D

S

A

A

Q
x
P

K

L

S

T

V

V

L

L

E

P

A

T

I

I

N

T

T

C

-

G

-

H

-

T

-

I

-

E

-

I

I

L

K

R

S

E

M

K

N
x
G

I
x
F

S
x
D

Q
|
Q

I

A

P

P

V

V

T

V

Q

D

Q

E

-

A

G

G

M

V

I

I

V

L

G

G

K

M

I

V

A

T

E

L

S

G

D

N

I

M

L

L

S

S

A

S

L

L

E

A

N

G

P

K

G

V

L

Q

K

P

S

S

A

D

P

Q

I

V

S

G

E

K

I

V

M

I

T

Y

A

K

T

Q

F

F

P

K

F

Q

V

I

D

R

L

L

N

T

T

D

S

T

I

L

D

G

K

R

I

L

S

S

S

H

L

I

I

L

N

E

K

M

E

D

N

H

S

F

A

A

V

L

L

V

V

V

E

H

D

E

E

Q

T

M

G

-

K

V

I

F

E

G

I

V

T
|
T

Q

A

Y
x
I

D
|
D

I

L

N

N

A

F

I

V

S

A

G

A

active site: K77 (= K43), S105 (= S71), D237 (= D205), S305 (= S276)
binding protoporphyrin ix containing fe: A182 (≠ L150), P185 (≠ S153), L186 (≠ Q154), Y189 (= Y157), R222 (≠ K190), T269 (≠ A240)
binding pyridoxal-5'-phosphate: K77 (= K43), N107 (= N73), G212 (= G180), T213 (= T181), G214 (= G182), T216 (= T184), G261 (= G232), S305 (= S276), P331 (≠ H302), D332 (= D303)
binding s-adenosylmethionine: P376 (≠ Q349), G396 (≠ N363), F397 (≠ I364), D398 (≠ S365), Q399 (= Q366), T476 (= T443), I478 (≠ Y445), D479 (= D446)

Sites not aligning to the query:

binding protoporphyrin ix containing fe: 8, 9, 10, 11, 12, 20, 21, 22, 23

3pc4A Full length structure of cystathionine beta-synthase from drosophila in complex with serine (see paper)
36% identity, 99% coverage: 5:453/453 of query aligns to 42:500/504 of 3pc4A

query
sites
3pc4A

N

N

I

I

L

L

E

E

T

V

I

I

G

G

N

C

T

T

P

P

L

L

V

V

K

K

L

L

N

N

T

N

I

I

-

P

-

A

T

S

K

D

G

G

V

I

P

E

G

C

T

E

I

M

L

Y

A

A

K

K

I

C

E

E

T

F

T

L

N

N

P

P

G

G

N

G

S

S

I

V

K
|
K

D

D

R

R

M

I

A

G

V

Y

K

R

M

M

I

V

E

Q

D

D

A

A

E

E

K

E

S

Q

G

G

K

L

L

L

K

K

P

P

G

G

G

Y

T

T

I

I

E

E

G

P

T
|
T

S
|
S

G

G

N
|
N

T
|
T

G

G

M

I

G

G

L

L

A

A

M

M

A

A

A

C

I

A

I

V

K

K

G

G

Y

Y

K

K

C

C

I

I

F

I

T

V

T

M

T

P

D

E

K

K

Q

M

S

S

K

N

E

E

K

K

V

V

D

S

A

A

L

L

R

R

A

T

F

L

G

G

A

A

E

K

V

I

I

I

V

R

C

T

P

P

T

T

N

E

V

A

E

A

P

Y

E

D

D

S

P

P

R

E

S

G

Y

L

Y

I

S

Y

V

V

S

A

S

Q

R

Q

L

L

E

Q

R

R

E

E

V

T

P

P

N

N

S

S

W

I

K

V

P

L

N

D

Q
|
Q

Y

Y

D

R

N

N

L
x
A

A

G

N

N

S
x
P

Q
x
L

A

A

H

H

Y
|
Y

E

D

Q

G

T

T

G

A

P

A

E

E

I

I

W

L

A

W

Q

Q

T

L

E

D

G

N

K

K

I

V

T

D

H

M

L

I

V

V

G

S

V
x
A

G
|
G

T
|
T

G
x
A

G

G

T
|
T

I

I

S

S

G

G

T

I

G

G

K
x
R

Y

K

L

I

K

K

E

E

K

Q

N

V

P

P

N

S

I

C

K

Q

V

I

W

V

G

G

I

V

D

D

T

P

Y

Y

G

G

S

S

V

I

F

L

K

A

K

R

Y

P

K

A

E

E

T

-

G

-

I

-

F

L

D

N

K

K

D

T

E

D

I

V

Y

Q

P

F

Y

Y

I

E

T

V

E

E

G
|
G

I
|
I

G

G

E
x
Y

D

D

F

F

L

P

P

P

A
x
T

N

V

V

F

N

D

F

D

D

T

V

V

I

V

D

D

L

V

F

W

E

T

K

K

V

I

T

G

D

D

K

S

D

D

A

C

A

F

L

P

M

M

T

S

R

R

D

R

I

L

A

N

R

A

K

E

E

E

G

G

I

L

F

L

V

C

G

G

N

G

S
|
S

A

S

G

G

A

G

A

A

I

M

G

H

G

A

L

A

I

L

Q

E

L

H

K

A

D

R

K

K

L

L

K

K

P

K

E

G

D

Q

V

R

V

C

V

V

I

I

F

L

H
x
P

D
|
D

H

G

G

I

S

R

R

N

Y

Y

M

M

G

T

K

K

M

F

Y

V

N

S

E

D

D

N

W

W

L

M

R

E

E

A

R

R

G

N

F

F

L

K

Q

E

D

P

-

V

-

N

-

E

-

H

-

G

E

H

K

W

L

W

T

W

A

S

K

L

S

A

I

I

L

A

S

E

K

L

K

E

E

L

S

P

T

A

E

P

I

P

V

V

T

I

L

L

D

K

A

S

Q

D

K

A

S

T

V

V

L

G

E

E

A

A

I

I

N

A

T

L

I

M

K

K

S

K

M

H

N

R

I

V

S

D

Q

Q

I

L

P

P

V

V

T

V

Q

D

Q

Q

-

D

G

G

M

S

I

V

V

L

G

G

K

V

I

V

A

G

E

Q

S

E

D

T

I

L

L

I

S

T

A

Q

L

I

L

V

E

S

N

M

P

N

G

R

L

Q

K

Q

S

S

A

D

P

P

I

A

S

I

E

K

I

A

M

L

T

N

A

K

T

R

F

V

P

I

F

R

V

L

D

N

L

E

N

S

T

E

S

I

I

L

D

G

K

K

I

L

S

A

S

R

L

V

I

L

N

E

K

V

E

D

N

P

S

S

A

V

V

L

L

I

V

L

-

G

E

K

D

N

E

P

T

A

G

G

K

K

I

V

E

E

-

L

-

K

-

A

I

L

I

A

T

T

Q

K

Y

L

D

D

I

V

I

T

N

T

A

F

I

I

S

A

G

A

active site: K82 (= K43), S312 (= S276)
binding protoporphyrin ix containing fe: A189 (≠ L150), P192 (≠ S153), L193 (≠ Q154), Y196 (= Y157), R229 (≠ K190), T276 (≠ A240)
binding (E)-N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene)-L-serine: K82 (= K43), T109 (= T70), S110 (= S71), N112 (= N73), T113 (= T74), Q185 (= Q146), A218 (≠ V179), G219 (= G180), T220 (= T181), A221 (≠ G182), T223 (= T184), G268 (= G232), I269 (= I233), Y271 (≠ E235), S312 (= S276), P338 (≠ H302), D339 (= D303)

Sites not aligning to the query:

binding protoporphyrin ix containing fe: 14, 15, 16, 17, 18, 26, 27, 28

3pc3A Full length structure of cystathionine beta-synthase from drosophila in complex with aminoacrylate (see paper)
36% identity, 99% coverage: 5:453/453 of query aligns to 42:500/504 of 3pc3A

query
sites
3pc3A

N

N

I

I

L

L

E

E

T

V

I

I

G

G

N

C

T

T

P

P

L

L

V

V

K

K

L

L

N

N

T

N

I

I

-

P

-

A

T

S

K

D

G

G

V

I

P

E

G

C

T

E

I

M

L

Y

A

A

K

K

I

C

E

E

T

F

T

L

N

N

P

P

G

G

N

G

S

S

I

V

K
|
K

D

D

R

R

M

I

A

G

V

Y

K

R

M

M

I

V

E

Q

D

D

A

A

E

E

K

E

S

Q

G

G

K

L

L

L

K

K

P

P

G

G

G

Y

T

T

I

I

E

E

G

P

T
|
T

S
|
S

G

G

N
|
N

T
|
T

G

G

M

I

G

G

L

L

A

A

M

M

A

A

A

C

I

A

I

V

K

K

G

G

Y

Y

K

K

C

C

I

I

F

I

T

V

T

M

T

P

D

E

K

K

Q

M

S

S

K

N

E

E

K

K

V

V

D

S

A

A

L

L

R

R

A

T

F

L

G

G

A

A

E

K

V

I

I

I

V

R

C

T

P

P

T

T

N

E

V

A

E

A

P

Y

E

D

D

S

P

P

R

E

S

G

Y

L

Y

I

S

Y

V

V

S

A

S

Q

R

Q

L

L

E

Q

R

R

E

E

V

T

P

P

N

N

S

S

W

I

K

V

P

L

N

D

Q
|
Q

Y

Y

D

R

N

N

L
x
A

A

G

N

N

S
x
P

Q
x
L

A

A

H

H

Y
|
Y

E

D

Q

G

T

T

G

A

P

A

E

E

I

I

W

L

A

W

Q

Q

T

L

E

D

G

N

K

K

I

V

T

D

H

M

L

I

V

V

G

S

V
x
A

G
|
G

T
|
T

G
x
A

G

G

T
|
T

I

I

S

S

G

G

T

I

G

G

K
x
R

Y

K

L

I

K

K

E

E

K

Q

N

V

P

P

N

S

I

C

K

Q

V

I

W

V

G

G

I

V

D

D

T

P

Y

Y

G

G

S

S

V

I

F

L

K

A

K

R

Y

P

K

A

E

E

T

-

G

-

I

-

F

L

D

N

K

K

D

T

E

D

I

V

Y

Q

P

F

Y

Y

I

E

T

V

E

E

G
|
G

I
|
I

G

G

E

Y

D

D

F

F

L

P

P

P

A

T

N

V

V

F

N

D

F

D

D

T

V

V

I

V

D

D

L

V

F

W

E

T

K

K

V

I

T

G

D

D

K

S

D

D

A

C

A

F

L

P

M

M

T

S

R

R

D

R

I

L

A

N

R

A

K

E

E

E

G

G

I

L

F

L

V

C

G

G

N

G

S
|
S

A

S

G

G

A

G

A

A

I

M

G

H

G

A

L

A

I

L

Q

E

L

H

K

A

D

R

K

K

L

L

K

K

P

K

E

G

D

Q

V

R

V

C

V

V

I

I

F

L

H
x
P

D
|
D

H

G

G

I

S

R

R

N

Y

Y

M

M

G

T

K

K

M

F

Y

V

N

S

E

D

D

N

W

W

L

M

R

E

E

A

R

R

G

N

F

F

L

K

Q

E

D

P

-

V

-

N

-

E

-

H

-

G

E

H

K

W

L

W

T

W

A

S

K

L

S

A

I

I

L

A

S

E

K

L

K

E

E

L

S

P

T

A

E

P

I

P

V

V

T

I

L

L

D

K

A

S

Q

D

K

A

S

T

V

V

L

G

E

E

A

A

I

I

N

A

T

L

I

M

K

K

S

K

M

H

N

R

I

V

S

D

Q

Q

I

L

P

P

V

V

T

V

Q

D

Q

Q

-

D

G

G

M

S

I

V

V

L

G

G

K

V

I

V

A

G

E

Q

S

E

D

T

I

L

L

I

S

T

A

Q

L

I

L

V

E

S

N

M

P

N

G

R

L

Q

K

Q

S

S

A

D

P

P

I

A

S

I

E

K

I

A

M

L

T

N

A

K

T

R

F

V

P

I

F

R

V

L

D

N

L

E

N

S

T

E

S

I

I

L

D

G

K

K

I

L

S

A

S

R

L

V

I

L

N

E

K

V

E

D

N

P

S

S

A

V

V

L

L

I

V

L

-

G

E

K

D

N

E

P

T

A

G

G

K

K

I

V

E

E

-

L

-

K

-

A

I

L

I

A

T

T

Q

K

Y

L

D

D

I

V

I

T

N

T

A

F

I

I

S

A

G

A

active site: K82 (= K43), S312 (= S276)
binding protoporphyrin ix containing fe: A189 (≠ L150), P192 (≠ S153), L193 (≠ Q154), Y196 (= Y157), R229 (≠ K190)
binding 2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]acrylic acid: K82 (= K43), T109 (= T70), S110 (= S71), N112 (= N73), T113 (= T74), Q185 (= Q146), A218 (≠ V179), G219 (= G180), T220 (= T181), A221 (≠ G182), T223 (= T184), G268 (= G232), I269 (= I233), S312 (= S276), P338 (≠ H302), D339 (= D303)

Sites not aligning to the query:

binding protoporphyrin ix containing fe: 14, 15, 16, 17, 18, 26, 27, 28

3pc2A Full length structure of cystathionine beta-synthase from drosophila (see paper)
36% identity, 99% coverage: 5:453/453 of query aligns to 40:498/500 of 3pc2A

query
sites
3pc2A

N

N

I

I

L

L

E

E

T

V

I

I

G

G

N

C

T

T

P

P

L

L

V

V

K

K

L

L

N

N

T

N

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