SitesBLAST

4ea8A X-ray crystal structure of perb from caulobacter crescentus in complex with coenzyme a and gdp-n-acetylperosamine at 1 angstrom resolution (see paper)
33% identity, 93% coverage: 12:207/211 of query aligns to 16:206/207 of 4ea8A

query
sites
4ea8A

H
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H

A

A

A

K

E

V

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V

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Y

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N

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E

Y

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V

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I
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D

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N

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K

binding coenzyme a: H145 (≠ T146), P148 (= P149), F163 (= F164), G165 (= G166), V166 (≠ S167), G183 (≠ A184), A184 (= A185), L197 (≠ M198), I199 (≠ A200), K206 (= K207)
binding GDP-N-acetylperosamine: H16 (= H12), V35 (≠ I33), D36 (≠ N34), A37 (≠ L35), D38 (≠ E36), D50 (≠ I48), I68 (= I67), G69 (= G68), L73 (≠ E74), L77 (≠ I78), H145 (≠ T146)

Sites not aligning to the query:

binding GDP-N-acetylperosamine: 11, 12, 13, 14, 15

4eaaA X-ray crystal structure of the h141n mutant of perosamine n- acetyltransferase from caulobacter crescentus in complex with coa and gdp-perosamine (see paper)
35% identity, 76% coverage: 49:209/211 of query aligns to 53:210/210 of 4eaaA

query
sites
4eaaA

I

L

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I
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I

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binding coenzyme a: H147 (≠ T146), P150 (= P149), F165 (= F164), G167 (= G166), V168 (≠ S167), G185 (≠ A184), A186 (= A185), I201 (≠ A200), K208 (= K207)
binding GDP-perosamine: I70 (= I67), G71 (= G68), L75 (≠ E74), L79 (≠ I78), H147 (≠ T146)

Sites not aligning to the query:

binding GDP-perosamine: 11, 12, 13, 14, 15, 16, 35, 36, 37, 38, 52

4m99A Acetyltransferase domain of pglb from neisseria gonorrhoeae fa1090 in complex with acetyl coenzyme a (see paper)
30% identity, 96% coverage: 3:205/211 of query aligns to 4:204/206 of 4m99A

query
sites
4m99A

N

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S

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G

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N

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K

binding acetyl coenzyme *a: N129 (= N130), D135 (≠ S136), H136 (= H137), S147 (= S148), P148 (= P149), S153 (≠ A154), G154 (= G155), R163 (≠ F164), G165 (= G166), T166 (≠ S167), R171 (≠ I172), Q172 (≠ P173), G183 (≠ A184), A184 (= A185), V187 (≠ C188), V189 (≠ T190), A199 (= A200), G200 (= G201), N201 (≠ V202), P202 (= P203), K204 (≠ R205)

Sites not aligning to the query:

binding acetyl coenzyme *a: 206

7txsA X-ray structure of the viob n-aetyltransferase from acinetobacter baumannii in the presence of a reaction intermediate (see paper)
31% identity, 99% coverage: 1:208/211 of query aligns to 1:206/209 of 7txsA

query
sites
7txsA

M

M

I

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H

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I

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L

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K

binding [(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-4-hydroxy-3-(phosphonooxy)oxolan-2-yl]methyl (3R)-4-({3-[(2-{[(1S)-1-{[(2R,3S,4S,5R,6R)-4,5-dihydroxy-6-{[(R)-hydroxy{[(R)-hydroxy{[(2R,3S,5R)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)oxolan-2-yl]methoxy}phosphoryl]oxy}phosphoryl]oxy}-2-methyloxan-3-yl]amino}ethyl]sulfanyl}ethyl)amino]-3-oxopropyl}amino)-3-hydroxy-2,2-dimethyl-4-oxobutyl dihydrogen diphosphate (non-preferred name): G10 (= G10), G11 (≠ K11), H12 (= H12), D32 (≠ E36), N33 (≠ D37), T34 (≠ A38), K37 (≠ H41), L51 (= L55), I66 (= I70), G67 (= G71), K74 (≠ S77), I75 (= I78), N128 (= N130), A146 (≠ S148), P147 (= P149), G164 (= G166), A165 (≠ S167), M180 (≠ Y182), G182 (≠ A184), M183 (≠ A185), V198 (≠ A200), G199 (= G201), L205 (≠ K207)

7txqA X-ray structure of the viob n-acetyltransferase from acinetobacter baumannii in the present of tdp and acetyl-coenzymea (see paper)
31% identity, 99% coverage: 1:208/211 of query aligns to 1:206/209 of 7txqA

query
sites
7txqA

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L

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K

binding acetyl coenzyme *a: A146 (≠ S148), P147 (= P149), G164 (= G166), A165 (≠ S167), M180 (≠ Y182), G182 (≠ A184), M183 (≠ A185), V198 (≠ A200), G199 (= G201), L205 (≠ K207), K206 (= K208)
binding thymidine-5'-diphosphate: G10 (= G10), G11 (≠ K11), H12 (= H12), D32 (≠ E36), N33 (≠ D37), T34 (≠ A38), K37 (≠ H41), L51 (= L55), I66 (= I70), G67 (= G71), I75 (= I78)

7txpA X-ray structure of the viob n-acetyltransferase from acinetobacter baumannii in complex with tdp-4-amino-4,6-dideoxy-d-glucose (see paper)
31% identity, 99% coverage: 1:208/211 of query aligns to 1:206/209 of 7txpA

query
sites
7txpA

M

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I

V

G

I

A

A

I
|
I

G
|
G

N

S

-

P

Y

R

E

A

R

R

K

K

S
x
K

I
|
I

I

I

E

E

P

H

L

F

E

F

N

P

K

E

G

G

-

E

Y

F

P

T

F

F

I

A

N

T

I

L

I

I

H

D

H

P

H

T

N

A

Y

T

I

I

S

G

S

E

S

N

I

I

K

H

I

I

G

E

E

E

G

G

N

T

C

M

I

I

A

C

P

A

G

G

C

G

V

I

I

L

N

T

A

V

N

D

V

V

R

K

I

L

G

G

N

K

H

H

C

C

I

I

V

V

N

N

S

T

N

N

C

A

N

V

I

L

S

S

H

H

D

G

C

V

I

I

L

L

E

G

N

D

Y

Y

V

V

T

T

I

V

S

A

P

P

G

N

V

A

T

S

I

I

A

S

G

G

N

D

V

V

H

S

I

L

H

G

E

N

G

I

V

V

F

E

I

I

G

G

S

A

G

N

A

A

T

T

V

I

I

R

P

E

G

K

I

V

S

S

I

V

G

Q

K

D

G

G

A

A

Y

M

V

V

A

G

A

M

G

G

A

S

C

V

V

V

T

I

K

R

D

N

V

I

P

L

P

S

H

N

V

Q

M

V

V

V

A

V

G

G

V

N

P

P

A

A

R

K

V

L

K

L

K

K

binding dTDP-4-amino-4,6-dideoxyglucose: G10 (= G10), G11 (≠ K11), H12 (= H12), D32 (≠ E36), N33 (≠ D37), T34 (≠ A38), K37 (≠ H41), L51 (= L55), I66 (= I70), G67 (= G71), K74 (≠ S77), I75 (= I78)

7l82AAA Putative acetyl transferase protein (see paper)
38% identity, 63% coverage: 75:206/211 of query aligns to 79:215/216 of 7l82AAA

query
sites
7l82AAA

R

R

K

Q

S

K

I
|
I

I

A

E

D

P

K

L

L

E

V

N

K

K

D

G

G

Y

I

P

S

F

L

I

W

N

T

I

V

I

Q

H

G

H

M

H

T

N

T

Y

L

I

I

S

M

S

D

S

E

I

V

K

S

I

I

G

D

E

A

G

G

N

A

C

A

I

L

A

S

P

P

G

F

C

V

V

T

I

I

N

A

A

A

N

N

V

V

R

T

I

I

G

G

N

K

H

C

C

F

I

H

V

A

N
|
N

S

L

N

Y

C

S

N

Y

I

V

S

E

H

H

D

D

C

C

I

I

L

I

E

G

N

D

Y

Y

V

V

T

T

I

F

S
x
A

P
|
P

G

R

V

V

T

S

I

C

A

N

G

G

N

N

V

I

H

H

I

I

H

H

E

D

G

H

V

A

F
x
Y

I

I

G
|
G

S
x
T

G

G

A

A

T

V

V

I

I

K

P

Q

G

G

-

T

-

P

-

D

-

K

-

P

I

L

S

I

I

I

G

G

K

K

G

G

A

A

Y

I

V

V

A
x
G

A
x
M

G

G

A

A

C

V

V

V

T

T

K

K

D

E

V

V

P

P

P

A

H

G

V

A

M

V

V

V

A
x
I

G

G

V

N

P

P

A

A

R

R

V

L

binding [(2~{R},3~{R},4~{S},5~{S},6~{R})-3-acetamido-5-[[(1~{S})-1-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethylsulfanyl]-1-oxidanyl-ethyl]amino]-6-methyl-4-oxidanyl-oxan-2-yl] [[(2~{R},3~{S},4~{R},5~{R})-5-[2,4-bis(oxidanylidene)pyrimidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] hydrogen phosphate: I82 (= I78), N134 (= N130), A152 (≠ S148), P153 (= P149), Y168 (≠ F164), G170 (= G166), T171 (≠ S167), G193 (≠ A184), M194 (≠ A185), I209 (≠ A200)

Sites not aligning to the query:

binding [(2~{R},3~{R},4~{S},5~{S},6~{R})-3-acetamido-5-[[(1~{S})-1-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethylsulfanyl]-1-oxidanyl-ethyl]amino]-6-methyl-4-oxidanyl-oxan-2-yl] [[(2~{R},3~{S},4~{R},5~{R})-5-[2,4-bis(oxidanylidene)pyrimidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] hydrogen phosphate: 7, 10, 11, 12, 39, 40, 41, 73, 74, 78

7l81AAA Putative acetyl transferase protein (see paper)
38% identity, 63% coverage: 75:206/211 of query aligns to 79:215/216 of 7l81AAA

query
sites
7l81AAA

R

R

K

Q

S

K

I
|
I

I

A

E

D

P

K

L

L

E

V

N

K

K

D

G

G

Y

I

P

S

F

L

I

W

N

T

I

V

I

Q

H

G

H

M

H

T

N

T

Y

L

I

I

S

M

S

D

S

E

I

V

K

S

I

I

G

D

E

A

G

G

N

A

C

A

I

L

A

S

P

P

G

F

C

V

V

T

I

I

N

A

A

A

N

N

V

V

R

T

I

I

G

G

N

K

H

C

C

F

I

H

V

A

N

N

S

L

N

Y

C

S

N

Y

I

V

S

E

H

H

D

D

C

C

I

I

L

I

E

G

N

D

Y

Y

V

V

T

T

I

F

S
x
A

P
|
P

G

R

V

V

T

S

I

C

A

N

G

G

N

N

V

I

H

H

I

I

H

H

E

D

G

H

V

A

F
x
Y

I

I

G
|
G

S
x
T

G

G

A

A

T

V

V

I

I

K

P

Q

G

G

-

T

-

P

-

D

-

K

-

P

I

L

S

I

I

I

G

G

K

K

G

G

A

A

Y

I

V

V

A
x
G

A
x
M

G

G

A

A

C

V

V

V

T

T

K

K

D

E

V

V

P

P

P

A

H

G

V

A

M

V

V

V

A
x
I

G

G

V

N

P

P

A

A

R

R

V

L

binding coenzyme a: A152 (≠ S148), P153 (= P149), Y168 (≠ F164), G170 (= G166), T171 (≠ S167), G193 (≠ A184), M194 (≠ A185), I209 (≠ A200)
binding UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose: I82 (= I78)

Sites not aligning to the query:

binding UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose: 7, 10, 11, 12, 39, 40, 41, 73, 74, 78

7l7zAAA Putative acetyl transferase protein (see paper)
38% identity, 63% coverage: 75:206/211 of query aligns to 79:215/216 of 7l7zAAA

query
sites
7l7zAAA

R

R

K

Q

S

K

I
|
I

I

A

E

D

P

K

L

L

E

V

N

K

K

D

G

G

Y

I

P

S

F

L

I

W

N

T

I

V

I

Q

H

G

H

M

H

T

N

T

Y

L

I

I

S

M

S

D

S

E

I

V

K

S

I

I

G

D

E

A

G

G

N

A

C

A

I

L

A

S

P

P

G

F

C

V

V

T

I

I

N

A

A

A

N

N

V

V

R

T

I

I

G

G

N

K

H

C

C

F

I

H

V

A

N

N

S

L

N

Y

C

S

N

Y

I

V

S

E

H

H

D

D

C

C

I

I

L

I

E

G

N

D

Y

Y

V

V

T

T

I

F

S
x
A

P
|
P

G

R

V

V

T

S

I

C

A

N

G

G

N

N

V

I

H

H

I

I

H

H

E

D

G

H

V

A

F
x
Y

I

I

G
|
G

S
x
T

G

G

A

A

T

V

V

I

I

K

P

Q

G

G

-

T

-

P

-

D

-

K

-

P

I

L

S

I

I

I

G

G

K

K

G

G

A

A

Y

I

V

V

A
x
G

A
x
M

G

G

A

A

C

V

V

V

T

T

K

K

D

E

V

V

P

P

P

A

H

G

V

A

M

V

V

V

A
x
I

G

G

V

N

P

P

A

A

R

R

V

L

binding coenzyme a: A152 (≠ S148), P153 (= P149), Y168 (≠ F164), G170 (= G166), T171 (≠ S167), G193 (≠ A184), M194 (≠ A185), I209 (≠ A200)
binding UDP-di-N-acetyl-alpha-bacillosamine: I82 (= I78)

Sites not aligning to the query:

binding UDP-di-N-acetyl-alpha-bacillosamine: 7, 10, 11, 12, 39, 40, 41, 73, 74, 78

7l7yAAA Putative acetyl transferase protein (see paper)
38% identity, 63% coverage: 75:206/211 of query aligns to 79:215/217 of 7l7yAAA

query
sites
7l7yAAA

R

R

K

Q

S

K

I

I

I

A

E

D

P

K

L

L

E

V

N

K

K

D

G

G

Y

I

P

S

F

L

I

W

N

T

I

V

I

Q

H

G

H

M

H

T

N

T

Y

L

I

I

S

M

S

D

S

E

I

V

K

S

I

I

G

D

E

A

G

G

N

A

C

A

I

L

A

S

P

P

G

F

C

V

V

T

I

I

N

A

A

A

N

N

V

V

R

T

I

I

G

G

N

K

H

C

C

F

I

H

V

A

N

N

S

L

N

Y

C

S

N

Y

I

V

S

E

H

H

D

D

C

C

I

I

L

I

E

G

N

D

Y

Y

V

V

T

T

I

F

S
x
A

P

P

G

R

V

V

T

S

I

C

A

N

G

G

N

N

V

I

H

H

I

I

H

H

E

D

G

H

V

A

F
x
Y

I

I

G
|
G

S
x
T

G

G

A

A

T

V

V

I

I

K

P

Q

G

G

-

T

-

P

-

D

-

K

-

P

I

L

S

I

I

I

G

G

K

K

G

G

A

A

Y

I

V

V

A
x
G

A
x
M

G

G

A

A

C

V

V

V

T

T

K

K

D

E

V

V

P

P

P

A

H

G

V

A

M

V

V

V

A
x
I

G

G

V

N

P

P

A

A

R

R

V

L

binding acetyl coenzyme *a: A152 (≠ S148), Y168 (≠ F164), G170 (= G166), T171 (≠ S167), G193 (≠ A184), M194 (≠ A185), I209 (≠ A200)

Sites not aligning to the query:

binding acetyl coenzyme *a: 216
binding uridine-5'-diphosphate: 7, 8, 10, 11, 12, 39, 40, 41, 73, 74, 78

5t2yA Crystal structure of c. Jejuni pgld in complex with 5-methyl-4- (methylamino)-2-phenethylthieno[2,3-d]pyrimidine-6-carboxylic acid (see paper)
33% identity, 76% coverage: 46:205/211 of query aligns to 28:190/192 of 5t2yA

query
sites
5t2yA

K

K

P

E

I

C

I

I

S

F

L

L

P

D

S

D

F

F

L

M

E

K

K

F

H

E

P

S

T

T

T

L

L

P

N

K

I

Y

A

D

L

F

I

F

G

I

A

A

I

I

G

G

N

N

Y

N

E

E

-

I

R

R

K

K

S

K

I

I

I

Y

E

Q

P

K

L

I

E

S

N

E

K

N

G

G

Y

F

P

K

F

I

I

V

N

N

I

L

I

I

H

H

H

K

H

S

N

A

Y

L

I

I

S

S

S

P

S

S

I

A

K

I

I

V

G

E

E

E

-

N

-

A

G

G

N

I

C

L

I

I

A

M

P

P

G

Y

C

V

V

V

I

I

N

N

A

A

N

K

V

A

R

K

I

I

G

E

N

K

H

G

C

V

I

I

V

L

N

N

S

T

N

S

C

S

N

V

I

I

S

E

H

H

D

E

C

C

I

V

L

I

E

G

N

E

Y

F

V

S

T

H

I

V

S
|
S

P

V

G

G

V

A

T

K

I

C

A

A

G

G

N

N

V

V

H

K

I

I

H

G

E

K

G

N

V

C

F
|
F

I

L

G
|
G

S
x
I

G

N

A

S

T

C

V

V

I

L

P

P

G

N

I

L

S

S

I

L

G

A

K

D

G

D

A

S

Y

I

V

L

A
x
G

G

G

A

A

C

T

V

L

T

V

K

K

D

N

V

Q

P

D

P

E

H

K

V

G

M

V

V

F

A
x
V

G

G

V

V

P

P

A

A

R

K

binding 5-methyl-4-(methylamino)-2-(2-phenylethyl)thieno[2,3-d]pyrimidine-6-carboxylic acid: S133 (= S148), F149 (= F164), G151 (= G166), I152 (≠ S167), G169 (≠ A184), G170 (≠ A185), V185 (≠ A200)

5tyhA Pgld from campylobacter jejuni nctc 11168 in complex with 5- furanyl)-1h-pyrazole-3-carboxylic acid (see paper)
33% identity, 72% coverage: 54:205/211 of query aligns to 29:183/185 of 5tyhA

query
sites
5tyhA

F

F

L

L

E

D

K

F

H

E

P

S

T

T

T

L

L

P

N

K

I

Y

A

D

L

F

I

F

G

I

A

A

I

I

G

G

N

N

Y

N

E

E

-

I

R

R

K

K

S

K

I

I

I

Y

E

Q

P

K

L

I

E

S

N

E

K

N

G

G

Y

F

P

K

F

I

I

V

N

N

I

L

I

I

H

H

H

K

H

S

N

A

Y
x
L

I
|
I

S
|
S

S

P

S

S

I

A

K

I

I

V

G

E

E

E

-

N

-

A

G

G

N

I

C

L

I

I

A

M

P

P

G

Y

C

V

V

V

I
|
I

N
|
N

A

A

N

K

V

A

R

K

I

I

G

E

N

K

H

G

C

V

I

I

V

L

N
|
N

S

T

N

S

C

S

N

V

I

I

S

E

H

H

D

E

C

C

I

V

L

I

E

G

N

E

Y

F

V

S

T
x
H

I

V

S
|
S

P
x
V

G

G

V

A

T

K

I

C

A

A

G

G

N

N

V

V

H

K

I

I

H

G

E

K

G

N

V

C

F
|
F

I

L

G
|
G

S
x
I

G

N

A

S

T

C

V

V

I

L

P

P

G

N

I

L

S

S

I

L

G

A

K

D

G

D

A

S

Y

I

V

L

A

G

A
x
G

G

G

A

A

C

T

V

L

T

V

K

K

D

N

V

Q

P

D

P

E

H

K

V

G

M

V

V

F

A

V

G

G

V

V

P

P

A

A

R

K

binding 3-(furan-2-yl)-1H-pyrazole-5-carboxylic acid: L74 (≠ Y98), I75 (= I99), S76 (= S100), I95 (= I117), N96 (= N118), N108 (= N130), H124 (≠ T146), S126 (= S148), V127 (≠ P149), F142 (= F164), G144 (= G166), I145 (≠ S167), G163 (≠ A185)

3bssA Pgld from campylobacter jejuni, nctc 11168, with native substrate (see paper)
34% identity, 65% coverage: 69:205/211 of query aligns to 53:192/194 of 3bssA

query
sites
3bssA

A

A

I
|
I

G
|
G

N

N

Y

N

E

E

-
x
I

R

R

K

K

S

K

I

I

I

Y

E

Q

P

K

L

I

E

S

N

E

K

N

G

G

Y

F

P

K

F

I

I

V

N

N

I

L

I

I

H

H

H

K

H

S

N

A

Y

L

I

I

S

S

S

P

S

S

I

A

K

I

I

V

G

E

E

E

-

N

-

A

G

G

N

I

C

L

I

I

A

M

P

P

G

Y

C

V

V

V

I

I

N

N

A

A

N

K

V

A

R

K

I

I

G

E

N

K

H

G

C

V

I

I

V

L

N

N

S

T

N

S

C

S

N

V

I

I

S

E

H

H

D

E

C

C

I

V

L

I

E

G

N

E

Y

F

V

S

T

H

I

V

S

S

P

V

G

G

V

A

T

K

I

C

A

A

G

G

N

N

V

V

H

K

I

I

H

G

E

K

G

N

V

C

F

F

I

L

G

G

S

I

G

N

A

S

T

C

V

V

I

L

P

P

G

N

I

L

S

S

I

L

G

A

K

D

G

D

A

S

Y

I

V

L

A

G

G

G

A

A

C

T

V

L

T

V

K

K

D

N

V

Q

P

D

P

E

H

K

V

G

M

V

V

F

A

V

G

G

V

V

P

P

A

A

R

K

binding UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose: I54 (= I70), G55 (= G71), I59 (vs. gap)

Sites not aligning to the query:

binding UDP-2-acetamido-4-amino-2,4,6-trideoxy-alpha-D-glucopyranose: 9, 11, 12, 13, 14, 34, 35, 36, 37

2vheA Pgld-coa complex: an acetyl transferase from campylobacter jejuni (see paper)
34% identity, 65% coverage: 69:205/211 of query aligns to 53:192/194 of 2vheA

query
sites
2vheA

A

A

I

I

G

G

N

N

Y

N

E

E

-

I

R

R

K

K

S

K

I

I

I

Y

E

Q

P

K

L

I

E

S

N

E

K

N

G

G

Y

F

P

K

F

I

I

V

N

N

I

L

I

I

H

H

H

K

H

S

N

A

Y

L

I

I

S

S

S

P

S

S

I

A

K

I

I

V

G

E

E

E

-

N

-

A

G

G

N

I

C

L

I

I

A

M

P

P

G

Y

C

V

V

V

I

I

N

N

A

A

N

K

V

A

R

K

I

I

G

E

N

K

H

G

C

V

I

I

V

L

N

N

S

T

N

S

C

S

N

V

I

I

S

E

H

H

D

E

C

C

I

V

L

I

E

G

N

E

Y

F

V

S

T

H

I

V

S
|
S

P
x
V

G

G

V

A

T

K

I

C

A

A

G

G

N

N

V

V

H

K

I

I

H

G

E

K

G

N

V

C

F
|
F

I

L

G
|
G

S
x
I

G

N

A

S

T

C

V

V

I

L

P

P

G

N

I

L

S

S

I

L

G

A

K

D

G

D

A

S

Y

I

V

L

A
x
G

G

G

A

A

C

T

V

L

T

V

K

K

D

N

V

Q

P

D

P

E

H

K

V

G

M

V

V

F

A
x
V

G
|
G

V

V

P

P

A

A

R

K

binding coenzyme a: S135 (= S148), V136 (≠ P149), F151 (= F164), G153 (= G166), I154 (≠ S167), G171 (≠ A184), G172 (≠ A185), V187 (≠ A200), G188 (= G201)

Sites not aligning to the query:

binding coenzyme a: 194

3bsyA Pgld from campylobacter jejuni, nctc 11168, in complex with acetyl coenzyme a (see paper)
34% identity, 65% coverage: 69:205/211 of query aligns to 52:191/193 of 3bsyA

query
sites
3bsyA

A

A

I

I

G

G

N

N

Y

N

E

E

-

I

R

R

K

K

S

K

I

I

I

Y

E

Q

P

K

L

I

E

S

N

E

K

N

G

G

Y

F

P

K

F

I

I

V

N

N

I

L

I

I

H

H

H

K

H

S

N

A

Y

L

I

I

S

S

S

P

S

S

I

A

K

I

I

V

G

E

E

E

-

N

-

A

G

G

N

I

C

L

I

I

A

M

P

P

G

Y

C

V

V

V

I

I

N

N

A

A

N

K

V

A

R

K

I

I

G

E

N

K

H

G

C

V

I

I

V

L

N
|
N

S

T

N

S

C

S

N

V

I

I

S

E

H

H

D

E

C

C

I

V

L

I

E

G

N

E

Y

F

V

S

T
x
H

I

V

S
|
S

P
x
V

G

G

V

A

T

K

I

C

A
|
A

G

G

N

N

V

V

H

K

I

I

H

G

E

K

G

N

V

C

F
|
F

I

L

G
|
G

S
x
I

G

N

A

S

T

C

V

V

I
x
L

P
|
P

G

N

I

L

S

S

I

L

G

A

K

D

G

D

A

S

Y

I

V

L

A
x
G

G

G

A

A

C

T

V

L

T
x
V

K

K

D

N

V

Q

P

D

P

E

H

K

V

G

M

V

V

F

A
x
V

G

G

V

V

P
|
P

A

A

R

K

binding acetyl coenzyme *a: N116 (= N130), H132 (≠ T146), S134 (= S148), V135 (≠ P149), A140 (= A154), F150 (= F164), G152 (= G166), I153 (≠ S167), L158 (≠ I172), P159 (= P173), G170 (≠ A184), G171 (≠ A185), V176 (≠ T190), V186 (≠ A200), P189 (= P203)

Sites not aligning to the query:

binding acetyl coenzyme *a: 193

Q0P9D1 UDP-N-acetylbacillosamine N-acetyltransferase; Protein glycosylation D; UDP-4-amino-4,6-dideoxy-N-acetyl-alpha-D-glucosamine N-acetyltransferase; EC 2.3.1.203 from Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168) (see 2 papers)
34% identity, 65% coverage: 69:205/211 of query aligns to 54:193/195 of Q0P9D1

query
sites
Q0P9D1

A

A

I

I

G

G

N

N

Y

N

E

E

-

I

R

R

K

K

S

K

I

I

I

Y

E

Q

P

K

L

I

E

S

N

E

K

N

G

G

Y

F

P

K

F

I

I

V

N

N

I

L

I

I

H

H

H

K

H

S

N

A

Y

L

I

I

S

S

S

P

S

S

I

A

K

I

I

V

G

E

E

E

-

N

-

A

G

G

N

I

C

L

I

I

A

M

P

P

G

Y

C

V

V

V

I

I

N

N

A

A

N

K

V

A

R

K

I

I

G

E

N

K

H

G

C

V

I

I

V

L

N
|
N

S

T

N

S

C

S

N

V

I

I

S
x
E

H
|
H

D

E

C

C

I

V

L

I

E

G

N

E

Y

F

V

S

T
x
H

I

V

S

S

P

V

G

G

V

A

T

K

I

C

A

A

G

G

N

N

V

V

H

K

I

I

H

G

E

K

G

N

V

C

F

F

I

L

G

G

S
x
I

G

N

A

S

T

C

V

V

I

L

P

P

G

N

I

L

S

S

I

L

G

A

K

D

G

D

A

S

Y

I

V

L

A

G

A
x
G

G

G

A

A

C

T

V

L

T

V

K

K

D

N

V

Q

P

D

P

E

H

K

V

G

M

V

V

F

A

V

G

G

V

V

P

P

A

A

R

K

N118 (= N130) mutation to A: Reduction in catalytic activity.
E124 (≠ S136) mutation to A: Reduction in catalytic activity.
H125 (= H137) active site, Proton acceptor; mutation to A: Strong reduction in catalytic activity.
H134 (≠ T146) binding ; mutation to A: Slight reduction in catalytic activity.
I155 (≠ S167) binding
G173 (≠ A185) binding

Sites not aligning to the query:

15 H→A: Induces a higher KM and approximate 3-fold decrease in the turnover number.

4mzuB Crystal structure of fdtd, a bifunctional ketoisomerase/n- acetyltransferase from shewanella denitrificans (see paper)
34% identity, 50% coverage: 101:206/211 of query aligns to 9:139/290 of 4mzuB

query
sites
4mzuB

S

Q

S

S

I

Q

K

N

I

I

G

G

E

D

G

N

N

T

C

K

I

V

A
x
W

P
x
Q

G

F

C

C

V
|
V

I

I

N
x
L

A

A

N

G

V

A

R

V

I

I

G

G

N

R

H

N

C

C

I

N

V

I

N
x
C

S
x
A

N

N

C

S

N

L

I

I

S

E

H

N

D

D

C

V

I

V

L

I

E

G

N

D

Y

N

V

V

T

T

I

I

S

K

P

S

G
|
G

V

V

T

Q

I

I

A

W

G

D

N

G

V

I

H

H

I

I

H

Q

E

D

G

D

V

V

F
|
F

I

I

G

G

-

P

-
x
N

-

V

-

T

-

F

-
x
T

-
x
N

-
x
D

-
x
K

-
x
Q

-
x
P

-

R

-

S

-

L

-

K

-

T

-

I

-

V

-

K

-

G

-

A

-

S

-

I

-
x
G

S
x
A

G

N

A

S

T

T

V

I

I
x
L

P

P

G

G

I

I

S

L

I

I

G

G

K

E

G

N

A

A

Y

M

V

V

A
x
G

A
|
A

G

G

A

A

C
x
V

V

I

T
|
T

K
|
K

D

N

V

V

P

P

P

D

H

N

V

A

M

I

V

V

A
x
I

G

G

V
x
N

P
|
P

A

G

R
|
R

V

I

binding coenzyme a: F72 (= F164), T80 (vs. gap), N81 (vs. gap), D82 (vs. gap), K83 (vs. gap), Q84 (vs. gap), P85 (vs. gap), G99 (vs. gap), A100 (≠ S167), L105 (≠ I172), G117 (≠ A184), A118 (= A185), V121 (≠ C188), T123 (= T190), K124 (= K191), I133 (≠ A200), N135 (≠ V202), P136 (= P203), R138 (= R205)
binding magnesium ion: G58 (= G150), N76 (vs. gap)
binding thymine: W20 (≠ A112), Q21 (≠ P113), V24 (= V116), L26 (≠ N118), C38 (≠ N130), A39 (≠ S131)

Sites not aligning to the query:

binding coenzyme a: 199
binding thymidine-5'-diphosphate: 165, 171, 174, 176, 189, 191, 202, 203, 270, 275, 277

4mzuF Crystal structure of fdtd, a bifunctional ketoisomerase/n- acetyltransferase from shewanella denitrificans (see paper)
33% identity, 50% coverage: 101:206/211 of query aligns to 9:140/294 of 4mzuF

query
sites
4mzuF

S

Q

S

S

I

Q

K

N

I

I

G

G

E

D

G

N

N

T

C

K

I

V

A
x
W

P
x
Q

G

F

C

C

V
|
V

I

I

N
x
L

A

A

N

G

V

A

R

V

I

I

G

G

N

R

H

N

C

C

I

N

V

I

N
x
C

S
x
A

N

N

C

S

N

L

I

I

S

E

H

N

D

D

C

V

I

V

L

I

E

G

N

D

Y

N

V

V

T

T

I

I

S

K

P

S

G

G

V

V

T

Q

I

I

A

W

G

D

N

G

V

I

H

H

I

I

H

Q

E

D

G

D

V

V

F
|
F

I

I

G

G

-

P

-

N

-

V

-

T

-

F

-
x
T

-
x
N

-
x
D

-
x
K

-

Q

-
x
P

-

R

-

S

-

K

-

I

-

K

-

T

-

I

-

V

-

K

-

G

-

A

-

S

-

I

-
x
G

S
x
A

G

N

A

S

T

T

V

I

I
x
L

P

P

G

G

I

I

S

L

I

I

G

G

K

E

G

N

A

A

Y

M

V

V

A
x
G

A
|
A

G

G

A

A

C
x
V

V

I

T
|
T

K
|
K

D

N

V

V

P

P

P

D

H

N

V

A

M

I

V

V

A
x
I

G

G

V
x
N

P
|
P

A

G

R
|
R

V

I

binding coenzyme a: F72 (= F164), T80 (vs. gap), N81 (vs. gap), D82 (vs. gap), K83 (vs. gap), P85 (vs. gap), G100 (vs. gap), A101 (≠ S167), L106 (≠ I172), G118 (≠ A184), A119 (= A185), V122 (≠ C188), T124 (= T190), K125 (= K191), I134 (≠ A200), N136 (≠ V202), P137 (= P203), R139 (= R205)
binding thymine: W20 (≠ A112), Q21 (≠ P113), V24 (= V116), L26 (≠ N118), C38 (≠ N130), A39 (≠ S131)

Sites not aligning to the query:

binding thymidine-5'-diphosphate: 171, 174, 176, 189, 191, 202, 203, 205, 270, 275

Query Sequence

>CA265_RS05165 CA265_RS05165 hypothetical protein
MINVYLLGAGKHAAELSEYFNGSDEYLLTGYIINLEDAVNHPNLTKPIISLPSFLEKHPT
TLNIALIGAIGNYERKSIIEPLENKGYPFINIIHHHNYISSSIKIGEGNCIAPGCVINAN
VRIGNHCIVNSNCNISHDCILENYVTISPGVTIAGNVHIHEGVFIGSGATVIPGISIGKG
AYVAAGACVTKDVPPHVMVAGVPARVKKEVG

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory