SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing CA265_RS06120 FitnessBrowser__Pedo557:CA265_RS06120 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 13 hits to proteins with known functional sites (download)

P09148 Galactose-1-phosphate uridylyltransferase; Gal-1-P uridylyltransferase; UDP-glucose--hexose-1-phosphate uridylyltransferase; EC 2.7.7.12 from Escherichia coli (strain K12) (see 4 papers)
51% identity, 97% coverage: 10:351/351 of query aligns to 9:348/348 of P09148

query
sites
P09148

N

H

P

P

H

H

T

R

R

R

L

Y

N

N

I

P

L

L

T

T

G

G

E

Q

W

W

V

I

L

L

V

V

S

S

P

P

H

H

R
|
R

T
x
A

K
|
K

R
|
R

P

P

W

W

Q

Q

G

G

K

A

V

Q

E

E

D

T

V

P

T

A

P

K

D

Q

N

V

R

L

P

P

E

A

Y

H

D

D

P

P

K

D

C
|
C

Y

F

L

L

C
|
C

P

A

G

G

N

N

S

V

R

R

A
x
V

D

T

G

G

D

D

S

K

N

N

P

P

E

D

Y

Y

T

T

E

G

S

T

F

Y

V

V

F

F

N

T

N
|
N

D
|
D

F

F

A

A

L

L

L

M

E

S

D

D

T

T

P

P

A

D

G

A

N

P

M

E

N

S

E

H

H

D

D

P

L

L

L

M

V

R

A

C

S

Q

N

S

Q

A

R

R

G

G

L

T

C

S

K

R

V

V

I

I

S

C

F

F

S

S

P

P

K

D

H
|
H

H

S

L

K

T

T

L

L

P

P

E

E

M

L

S

S

V

V

K

A

A

A

I

L

T

T

A

E

V

I

V

V

N

K

V

T

W

W

Q

Q

N

E

E

Q

F

T

N

A

S

E

L

L

A

G

E

K

N

T

N

-

W

-

I

Y

K

P

Y

W

I

V

Q

Q

I

V

F

F

E

E

N
|
N

K

K

G

G

E

A

I

A

M

M

G
|
G

C
|
C

S
|
S

N

N

P

P

H
|
H

P

P

H
|
H

G

G

Q
|
Q

I

I

W

W

S

A

Q

N

G

S

D

F

I

L

P

P

L

N

E

E

I

A

A

E

K

R

E
|
E

T

D

E

R

R

L

Q

Q

K

K

S

E

Y

Y

Y

F

A

A

I

E

H

Q

K

K

R

S

S

P

L

M

L

L

S

V

D

D

Y

Y

L

V

K

Q

L

R

E

E

Q

L

K

A

K

D

K

G

E

S

R

R

I

T

I

V

F

V

E

E

N

T

D

E

H

H

F

W

A

L

V

A

L

V

V

V

P

P

F

Y

W

W

A

A

V

A

W

W

P

P

Y

F

E

E

T

T

M

L

I

L

S

P

K

K

R

A

H

H

V

V

N

L

S

R

I

I

R

T

L

D

F

L

T

T

E

D

A

A

E

Q

K

R

E

S

S

D

L

L

A

A

E

L

A

A

I

L

K

K

V

K

L

L

T

T

T

S

K

R

Y

Y

D

D

N

N

L

L

F

F

E

Q

T

C

S

S

F

F

P

P

Y

Y

S

S

A

M

G

G

M

W

H
|
H

Q

G

A

A

P

P

V

F

N

N

N

G

G

E

Y

E

Y

N

P

Q

E

H

W

W

H

Q

W

L

H
|
H

M

A

H
|
H

F

F

Y

Y

P

P

L

L

R

R

S

S

A

A

S

T

V

V

K

R

K
|
K

F
|
F

M

M

V

V

G

G

Y
|
Y

E
|
E

M

M

L

L

A

A

N

E

P

T

Q
|
Q

R

R

D

D

I

L

T

T

P

A

E

E

F

Q

A

A

N

E

R

R

L

L

K

R

E

A

M

V

S

S

A

D

T

I

H

H

Y

F

K

R

I

E

S

S

K

G

V

V

RAKR 28:31 (≠ RTKR 29:32) binding
C52 (= C53) binding ; mutation to S: Decreases enzyme activity 3000-fold.
C55 (= C56) binding ; mutation to S: Decreases enzyme activity 600-fold.
V61 (≠ A62) binding in other chain
ND 77:78 (= ND 78:79) binding in other chain
H115 (= H116) binding ; mutation to N: Decreases enzyme activity by 98%.
N153 (= N156) binding in other chain
GCS 159:161 (= GCS 162:164) binding in other chain
C160 (= C163) mutation C->S,A: Slight inhibition of enzymatic activity.
S161 (= S164) mutation to A: 7000-fold reduction in specific activity.
H164 (= H167) binding ; mutation to N: Decreases enzyme activity 10000-fold.
H166 (= H169) active site, Tele-UMP-histidine intermediate; mutation to G: Abolishes enzymatic activity.
Q168 (= Q171) binding in other chain
E182 (= E185) binding ; mutation to A: Decreases enzyme activity by about 50%. Abolishes iron binding, but has no effect on zinc binding.
H281 (= H284) binding
H296 (= H299) binding
H298 (= H301) binding
KF 311:312 (= KF 314:315) binding
YE 316:317 (= YE 319:320) binding
Q323 (= Q326) binding in other chain

1guqA Structure of nucleotidyltransferase complexed with udp-glucose (see paper)
50% identity, 97% coverage: 10:351/351 of query aligns to 8:347/347 of 1guqA

query
sites
1guqA

N

H

P

P

H

H

T

R

R

R

L

Y

N

N

I

P

L

L

T

T

G

G

E

Q

W

W

V

I

L

L

V

V

S

S

P

P

H

H

R
|
R

T

A

K

K

R
|
R

P

P

W
|
W

Q

Q

G

G

K

A

V

Q

E

E

D

T

V

P

T

A

P

K

D

Q

N

V

R

L

P

P

E

A

Y

H

D

D

P

P

K

D

C
|
C

Y
x
F

L

L

C
|
C

P

A

G

G

N

N

S

V

R

R

A
x
V

D

T

G

G

D

D

S

K

N

N

P

P

E

D

Y

Y

T

T

E

G

S

T

F

Y

V

V

F

F

N

T

N
|
N

D
|
D

F

F

A

A

L

L

L

M

E

S

D

D

T

T

P

P

A

D

G

A

N

P

M

E

N

S

E

H

H

D

D

P

L

L

L

M

V

R

A

C

S

Q

N

S

Q

A

R

R

G

G

L

T

C

S

K

R

V

V

I

I

S

C

F

F

S

S

P

P

K

D

H
|
H

H

S

L

K

T

T

L

L

P

P

E

E

M

L

S

S

V

V

K

A

A

A

I

L

T

T

A

E

V

I

V

V

N

K

V

T

W

W

Q

Q

N

E

E

Q

F

T

N

A

S

E

L

L

A

G

E

K

N

T

N

-

W

-

I

Y

K

P

Y

W

I

V

Q

Q

I

V

F
|
F

E
|
E

N
|
N

K
|
K

G

G

E

A

I

A

M

M

G
|
G

C
|
C

S
|
S

N

N

P

P

H
|
H

P

P

H
x
G

G

G

Q
|
Q

I

I

W

W

S

A

Q

N

G

S

D

F

I

L

P

P

L

N

E

E

I

A

A

E

K

R

E
|
E

T

D

E

R

R

L

Q

Q

K

K

S

E

Y

Y

Y

F

A

A

I

E

H

Q

K

K

R

S

S

P

L

M

L

L

S

V

D

D

Y

Y

L

V

K

Q

L

R

E

E

Q

L

K

A

K

D

K

G

E

S

R

R

I

T

I

V

F

V

E

E

N

T

D

E

H

H

F

W

A

L

V

A

L

V

V

V

P

P

F

Y

W

W

A

A

V

A

W

W

P

P

Y

F

E

E

T

T

M

L

I

L

S

P

K

K

R

A

H

H

V

V

N

L

S

R

I

I

R

T

L

D

F

L

T

T

E

D

A

A

E

Q

K

R

E

S

S

D

L

L

A

A

E

L

A

A

I

L

K

K

V

K

L

L

T

T

T

S

K

R

Y

Y

D

D

N

N

L

L

F

F

E

Q

T

C

S

S

F

F

P

P

Y

Y

S

S

A

M

G

G

M

W

H
|
H

Q

G

A

A

P

P

V

F

N

N

N

G

G

E

Y

E

Y

N

P

Q

E

H

W

W

H

Q

W

L

H
|
H

M

A

H
|
H

F

F

Y

Y

P

P

L

L

R

R

S

S

A

A

S

T

V

V

K

R

K
|
K

F
|
F

M

M

V

V

G

G

Y
|
Y

E
|
E

M

M

L

L

A

A

N

E

P

T

Q

Q

R

R

D

D

I

L

T

T

P

A

E

E

F

Q

A

A

N

E

R

R

L

L

K

R

E

A

M

V

S

S

A

D

T

I

H

H

Y

F

K

R

I

E

S

S

K

G

V

V

active site: C51 (= C53), C54 (= C56), H114 (= H116), N152 (= N156), S160 (= S164), H163 (= H167), G165 (≠ H169), Q167 (= Q171)
binding fe (iii) ion: E181 (= E185), H280 (= H284), H295 (= H299), H297 (= H301)
binding potassium ion: E151 (= E155), N152 (= N156), K153 (= K157), G165 (≠ H169)
binding uridine-5'-diphosphate-glucose: R27 (= R29), R30 (= R32), W32 (= W34), F52 (≠ Y54), V60 (≠ A62), N76 (= N78), D77 (= D79), F150 (= F154), N152 (= N156), G158 (= G162), C159 (= C163), S160 (= S164), Q167 (= Q171), K310 (= K314), F311 (= F315), Y315 (= Y319), E316 (= E320)
binding zinc ion: C51 (= C53), C54 (= C56), H114 (= H116), H163 (= H167)

1gupA Structure of nucleotidyltransferase complexed with udp-galactose (see paper)
50% identity, 97% coverage: 10:351/351 of query aligns to 8:347/347 of 1gupA

query
sites
1gupA

N

H

P

P

H

H

T

R

R

R

L

Y

N

N

I

P

L

L

T

T

G

G

E

Q

W

W

V

I

L

L

V

V

S

S

P

P

H

H

R
|
R

T

A

K

K

R
|
R

P

P

W

W

Q

Q

G

G

K

A

V

Q

E

E

D

T

V

P

T

A

P

K

D

Q

N

V

R

L

P

P

E

A

Y

H

D

D

P

P

K

D

C
|
C

Y
x
F

L

L

C
|
C

P

A

G

G

N

N

S

V

R
|
R

A
x
V

D

T

G

G

D

D

S

K

N

N

P

P

E

D

Y

Y

T

T

E

G

S

T

F

Y

V

V

F

F

N

T

N
|
N

D
|
D

F
|
F

A

A

L

L

L

M

E

S

D

D

T

T

P

P

A

D

G

A

N

P

M

E

N

S

E

H

H

D

D

P

L

L

L

M

V

R

A

C

S

Q

N

S

Q

A

R

R

G

G

L

T

C

S

K

R

V

V

I

I

S

C

F

F

S

S

P

P

K

D

H
|
H

H

S

L

K

T

T

L

L

P

P

E

E

M

L

S

S

V

V

K

A

A

A

I

L

T

T

A

E

V

I

V

V

N

K

V

T

W

W

Q

Q

N

E

E

Q

F

T

N

A

S

E

L

L

A

G

E

K

N

T

N

-

W

-

I

Y

K

P

Y

W

I

V

Q

Q

I

V

F
|
F

E

E

N
|
N

K
|
K

G

G

E

A

I

A

M

M

G
|
G

C
|
C

S
|
S

N

N

P

P

H
|
H

P

P

H
x
G

G

G

Q
|
Q

I

I

W
|
W

S

A

Q

N

G

S

D

F

I

L

P

P

L

N

E

E

I

A

A

E

K

R

E
|
E

T

D

E

R

R

L

Q

Q

K

K

S

E

Y

Y

Y

F

A

A

I

E

H

Q

K

K

R

S

S

P

L

M

L

L

S

V

D

D

Y

Y

L

V

K

Q

L

R

E

E

Q

L

K

A

K

D

K

G

E

S

R

R

I

T

I

V

F

V

E

E

N

T

D

E

H

H

F

W

A

L

V

A

L

V

V

V

P

P

F

Y

W

W

A

A

V

A

W

W

P

P

Y

F

E

E

T

T

M

L

I

L

S

P

K

K

R

A

H

H

V

V

N

L

S

R

I

I

R

T

L

D

F

L

T

T

E

D

A

A

E

Q

K

R

E

S

S

D

L

L

A

A

E

L

A

A

I

L

K

K

V

K

L

L

T

T

T

S

K

R

Y

Y

D

D

N

N

L

L

F

F

E

Q

T

C

S

S

F

F

P

P

Y

Y

S

S

A

M

G

G

M

W

H
|
H

Q

G

A

A

P

P

V

F

N

N

N

G

G

E

Y

E

Y

N

P

Q

E

H

W

W

H

Q

W

L

H
|
H

M

A

H
|
H

F

F

Y

Y

P

P

L

L

R

R

S

S

A

A

S

T

V

V

K

R

K
|
K

F
|
F

M

M

V
|
V

G
|
G

Y

Y

E
|
E

M

M

L

L

A

A

N

E

P

T

Q

Q

R

R

D

D

I

L

T

T

P

A

E

E

F

Q

A

A

N

E

R

R

L

L

K

R

E

A

M

V

S

S

A

D

T

I

H

H

Y

F

K

R

I

E

S

S

K

G

V

V

active site: C51 (= C53), C54 (= C56), H114 (= H116), N152 (= N156), S160 (= S164), H163 (= H167), G165 (≠ H169), Q167 (= Q171)
binding fe (iii) ion: E181 (= E185), H280 (= H284), H295 (= H299), H297 (= H301)
binding galactose-uridine-5'-diphosphate: R27 (= R29), R30 (= R32), F52 (≠ Y54), R59 (= R61), V60 (≠ A62), N76 (= N78), D77 (= D79), F78 (= F80), F150 (= F154), N152 (= N156), G158 (= G162), C159 (= C163), S160 (= S164), Q167 (= Q171), W169 (= W173), K310 (= K314), F311 (= F315), V313 (= V317), G314 (= G318), E316 (= E320)
binding potassium ion: N152 (= N156), K153 (= K157), G165 (≠ H169)
binding zinc ion: C51 (= C53), C54 (= C56), H114 (= H116), H163 (= H167)

1hxpA Nucleotide transferase (see paper)
50% identity, 97% coverage: 10:351/351 of query aligns to 8:340/340 of 1hxpA

query
sites
1hxpA

N

H

P

P

H

H

T

R

R

R

L

Y

N

N

I

P

L

L

T

T

G

G

E

Q

W

W

V

I

L

L

V

V

S

S

P

P

H

H

R

R

T

A

K

K

R

R

P

P

W

W

Q

E

G

G

K

A

V

V

E

-

D

-

V

-

T

-

P

-

D

-

N

-

R

L

P

P

E

A

Y

H

D

D

P

P

K

D

C
|
C

Y
x
F

L

L

C
|
C

P

A

G

G

N

N

S

V

R

R

A
x
V

D

T

G

G

D

D

S

K

N

N

P

P

E

D

Y

Y

T

T

E

G

S

T

F

Y

V

V

F

F

N

T

N
|
N

D
|
D

F

F

A

A

L

L

L

M

E

S

D

D

T

T

P

P

A

D

G

A

N

P

M

E

N

S

E

H

H

D

D

P

L

L

L

M

V

R

A

C

S

Q

N

S

Q

A

R

R

G

G

L

T

C

S

K

R

V

V

I

I

S

C

F

F

S

S

P

P

K

D

H
|
H

H

S

L

K

T

T

L

L

P

P

E

E

M

L

S

S

V

V

K

A

A

A

I

L

T

T

A

E

V

I

V

V

N

K

V

T

W

W

Q

Q

N

E

E

Q

F

T

N

A

S

E

L

L

A

G

E

K

N

T

N

-

W

-

I

Y

K

P

Y

W

I

V

Q

Q

I

V

F

F

E

E

N
|
N

K

K

G

G

E

A

I

A

M

M

G

G

C
|
C

S
|
S

N

N

P

P

H
|
H

P

P

H
|
H

G

G

Q
|
Q

I

I

W

W

S

A

Q

N

G

S

D

F

I

L

P

P

L

N

E

E

I

A

A

E

K

R

E
|
E

T

D

E

R

R

L

Q

Q

K

K

S

E

Y

Y

Y

F

A

A

I

E

H

Q

K

K

R

S

S

P

L

M

L

L

S

V

D

D

Y

Y

L

V

K

Q

L

R

E

E

Q

L

K

A

K

D

K

G

E

S

R

R

I

T

I

V

F

V

E

E

N

T

D

E

H

H

F

W

A

L

V

A

L

V

V

V

P

P

F

Y

W

W

A

A

V

A

W

W

P

P

Y

F

E

E

T

T

M

L

I

L

S

P

K

K

R

A

H

H

V

V

N

L

S

R

I

I

R

T

L

D

F

L

T

T

E

D

A

A

E

Q

K

R

E

S

S

D

L

L

A

A

E

L

A

A

I

L

K

K

V

K

L

L

T

T

T

S

K

R

Y

Y

D

D

N

N

L

L

F

F

E

Q

T
x
C

S
|
S

F

F

P

P

Y

Y

S

S

A

M

G

G

M

W

H
|
H

Q

G

A

A

P

P

V

F

N

N

N

G

G

E

Y

E

Y

N

P

Q

E

H

W

W

H

Q

W

L

H
|
H

M

A

H
|
H

F

F

Y

Y

P

P

L
|
L

L

L

R

R

S

S

A
|
A

S

T

V

V

K

R

K

K

F

F

M

M

V

V

G

G

Y

Y

E

E

M

M

L

L

A

A

N

E

P

T

Q

Q

R

R

D

D

I

L

T

T

P

A

E

E

F

Q

A

A

N

E

R

R

L

L

K

R

E

A

M

V

S

S

A

D

T

I

H

H

Y

F

K

R

I

E

S

S

K

G

V

V

active site: C44 (= C53), C47 (= C56), H107 (= H116), N145 (= N156), S153 (= S164), H156 (= H167), H158 (= H169), Q160 (= Q171)
binding beta-mercaptoethanol: N145 (= N156), C152 (= C163), Q160 (= Q171), C264 (≠ T275), S265 (= S276), L295 (= L306), A299 (= A310)
binding fe (iii) ion: E174 (= E185), H273 (= H284), H288 (= H299), H290 (= H301)
binding uridine-5'-monophosphate: F45 (≠ Y54), V53 (≠ A62), N69 (= N78), D70 (= D79)
binding zinc ion: C44 (= C53), C47 (= C56), H107 (= H116), H156 (= H167)

P07902 Galactose-1-phosphate uridylyltransferase; Gal-1-P uridylyltransferase; UDP-glucose--hexose-1-phosphate uridylyltransferase; EC 2.7.7.12 from Homo sapiens (Human) (see 22 papers)
50% identity, 99% coverage: 4:350/351 of query aligns to 23:370/379 of P07902

query
sites
P07902

T

T

F

F

E

R

L

A

D

N

S

D

N

H

P

Q

H

H

T
x
I

R

R

L
x
Y

N

N

I

P

L

L

T

Q

G

D

E

E

W

W

V

V

L

L

V
|
V

S

S

P

A

H

H

R

R

T

M

K

K

R

R

P

P

W

W

Q

Q

G

G

K

Q

V

V

E

E

D

P

V

Q

T

L

P
x
L

D

K

N

T

R

V

P

P

E

R

Y

H

D

D

P

P

K

L

C

N

Y

P

L
|
L

C

C

P

P

G

G

N

A

S

I

R

R

A

A

D

N

G

G

D

E

S

V

N

N

P

P

E

Q

Y

Y

T

D

E

S

S

T

F

F

V

L

F

F

N

D

N

N

D

D

F

F

A

P

A

A

L

L

L

Q

E

P

D

D

T

A

P

P

A

S

G

P

N

G

M

P

N

S

E

D

H

H

D

P

L

L

L

F

V

Q

A

A

S

K

N

S

Q

A

R

R

G

G

L

V

C

C

K

K

V

V

I

M

S

C

F

F

S
x
H

P

P

K

W

H
x
S

H

D

L

V

T
|
T

L

L

P

P

E

L

M
|
M

S

S

V

V

K

P

A

E

I

I

T
x
R

A

A

V

V

V
|
V

N

D

V

A

W

W

Q

A

N

S

E

V

F

T

N

E

S

E

L

L

A

G

E

A

N

Q

N

-

W

-

I

Y

K

P

Y

W

I
x
V

Q

Q

I
|
I

F
|
F

E

E

N

N

K

K

G
|
G

E

A

I

M

M

M

G

G

C

C

S

S

N

N

P

P

H

H

P
|
P

H
|
H

G

C

Q
|
Q

I

V

W

W

S

A

Q

S

G

S

D

F

I
x
L

P

P

L

-

E

D

I

I

A

A

K

Q

E
x
R

T
x
E

E

E

R

R

-

S

Q

Q

K

Q

S

A

Y

Y

Y

K

A

S

I

Q

H

H

K

G

R

E

S

P

L

L

S

M

D
x
E

Y

Y

L

S

K
x
R

L

Q

E

E

Q

L

K
x
L

K

R

K

K

E

E

R
|
R

I

L

I

V

F

L

E

T

N

S

D

E

H

H

F

W

A

L

V

V

L

L

V

V

P

P

F

F

W

W

A

A

V

T

W

W

P

P

Y

Y

E

Q

T

T

M

L

I

L

S

P

K

R

R
|
R

H

H

V

V

N

R

S

R

I

L

R

P

L

E

F

L

T

T

E

P

A

A

E

E

K

R

E

D

S

D

L

L

A

A

E

S

A
x
I

I

M

K

K

V

K

L

L

T

L

T

T

K
|
K

Y

Y

D

D

N

N

L
|
L

F

F

E
|
E

T

T

S

S

F

F

P

P

Y

Y

S

S

A

M

G

G

M

W

H
|
H

Q

G

A

A

P

P

V

T

N

G

N

S

G

E

Y

A

Y

G

P

A

E

N

W

W

-
x
N

H

H

W

W

-

Q

-

L

H
|
H

M

A

H
|
H

F

Y

Y

Y

P

P

L

L

L
|
L

R

R

S

S

A
|
A

S

T

V

V

K
x
R

K

K

F

F

M

M

V

V

G

G

Y

Y

E

E

M

M

L

L

A

A

N

Q

P

A

Q

Q

R

R

D

D

I

L

T
|
T

P

P

E

E

F

Q

A

A

N

E

R

R

L

L

K

R

E

A

M

L

S

P

A

E

T

V

H

H

Y

Y

K

H

I

L

S

G

K

Q

I32 (≠ T13) to N: in GALAC1; mild; dbSNP:rs111033644
Y34 (≠ L15) to N: in GALAC1; affects protein stability; dbSNP:rs111033836
V44 (= V25) to M: in GALAC1; reduced enzyme activity; dbSNP:rs111033647
L62 (≠ P43) to M: in dbSNP:rs1800461
L74 (= L55) to P: in GALAC1; reduced enzyme activity; dbSNP:rs111033663
H132 (≠ S113) to Q: in GALAC1; affects protein stability; dbSNP:rs367543256
S135 (≠ H116) to L: in GALAC1; about 5% of normal galactose uridylyltransferase activity; dbSNP:rs111033690
T138 (= T119) to M: in GALAC1; mild; dbSNP:rs111033686
M142 (= M123) to K: in GALAC1; 4% of normal activity; dbSNP:rs111033695
R148 (≠ T129) to W: in GALAC1; unstable protein; dbSNP:rs111033693
V151 (= V132) to A: in GALAC1; approximately 3% of normal activity; dbSNP:rs111033701
V168 (≠ I151) to L: in GALAC1; loss of activity; dbSNP:rs367543258
I170 (= I153) to T: in GALAC1; loss of activity; dbSNP:rs111033839
F171 (= F154) to S: in GALAC1; reduced enzyme activity; dbSNP:rs111033715
G175 (= G158) to D: in GALAC1; strongly reduces galactose uridylyltransferase activity; dbSNP:rs111033718
P185 (= P168) to H: in GALAC1; loss of activity; dbSNP:rs111033722
H186 (= H169) active site, Tele-UMP-histidine intermediate
Q188 (= Q171) to R: in GALAC1; most common mutation; 10% of normal galactose uridylyltransferase activity; impairs protein folding; dbSNP:rs75391579
L195 (≠ I178) to P: in GALAC1; no enzymatic activity; dbSNP:rs111033728
R201 (≠ E185) to C: in GALAC1; 2-fold decrease in activity; dbSNP:rs111033739
E202 (≠ T186) binding
E220 (≠ D203) to K: in GALAC1; 3-fold decrease in activity; dbSNP:rs111033747
R223 (≠ K206) to S: in GALAC1; 3-fold decrease in activity; dbSNP:rs111033750
L227 (≠ K210) to P: in GALAC1; results in no detectable protein in the soluble fraction; dbSNP:rs111033846
R231 (= R214) to H: in GALAC1; 15% of normal activity; dbSNP:rs111033754
R259 (= R242) to Q: in GALAC1; loss of activity; dbSNP:rs886042070; to W: in GALAC1; mild; dbSNP:rs786204763
I278 (≠ A261) to N: in GALAC1; 18-fold decrease in activity; dbSNP:rs111033778
K285 (= K268) to N: in GALAC1; severe; impairs protein folding; nearly abolishes enzyme activity; dbSNP:rs111033773
L289 (= L272) to F: in GALAC1; 3-fold decrease in activity; dbSNP:rs111033774
E291 (= E274) to V: in GALAC1; 2-fold decrease in activity; dbSNP:rs111033841
H301 (= H284) binding
N314 (vs. gap) to D: in GALAC1; allele Duarte; exists as allelic variants Duarte-1 and Duarte-2; Duarte-1 has normal or increased activity; Duarte-2 has activity reduced to about 35-45% of normal; dbSNP:rs2070074
H319 (= H299) binding
H321 (= H301) binding
L327 (= L307) to P: in GALAC1; results in no detectable protein in the soluble fraction; dbSNP:rs111033832
A330 (= A310) to V: in GALAC1; mild; dbSNP:rs111033804
R333 (≠ K313) to W: in GALAC1; no enzymatic activity; dbSNP:rs111033800
T350 (= T330) to A: in GALAC1; mild; dbSNP:rs111033817

1hxpB Nucleotide transferase (see paper)
49% identity, 97% coverage: 10:349/351 of query aligns to 8:329/329 of 1hxpB

query
sites
1hxpB

N

H

P

P

H
|
H

T

R

R

R

L

Y

N

N

I

P

L

L

T

T

G

G

E

Q

W

W

V

I

L

L

V

V

S

S

P

P

H

H

R

R

T

L

K

-

R

-

P

-

W

-

Q

-

G

-

K

-

V

-

E

-

D

-

V

-

T

-

P

-

D

-

N

-

R

-

P

P

E

A

Y

H

D

D

P

P

K

D

C
|
C

Y

F

L

L

C
|
C

P

A

G

G

N

N

S

V

R
|
R

A
x
V

D

T

G

G

D

D

S

K

N

N

P

P

E

D

Y

Y

T

T

E

G

S

T

F

Y

V

V

F
|
F

N

T

N
|
N

D
|
D

F

F

A

A

L

L

L

M

E

S

D

D

T

T

P

P

A

D

G

A

N

P

M

E

N

S

E

H

H

D

D

P

L

L

L

M

V

R

A

C

S

Q

N

S

Q

A

R

R

G

G

L

T

C

S

K

R

V

V

I

I

S

C

F

F

S

S

P

P

K

D

H
|
H

H

S

L

K

T

T

L

L

P

P

E

E

M

L

S

S

V

V

K

A

A

A

I

L

T

T

A

E

V

I

V

V

N

K

V

T

W

W

Q

Q

N

E

E

Q

F

T

N

A

S

E

L

L

A

G

E

K

N

T

N

-

W

-

I

Y

K

P

Y

W

I

V

Q

Q

I

V

F

F

E

E

N
|
N

K

K

G

G

E

A

I

A

M

M

G

G

C
|
C

S
|
S

N
|
N

P

P

H
|
H

P

P

H
|
H

G

G

Q
|
Q

I

I

W

W

S

A

Q

N

G

S

D

F

I

L

P

P

L

N

E

E

I

A

A

E

K

R

E
|
E

T

D

E

R

R

L

Q

Q

K

K

S

E

Y

Y

Y

F

A

A

I

E

H

Q

K

K

R

S

S

P

L

M

L

L

S

V

D

D

Y

Y

L

V

K

Q

L

R

E

E

Q

L

K

A

K

D

K

G

E

S

R

R

I

T

I

V

F

V

E

E

N

T

D

E

H

H

F

W

A

L

V

A

L

V

V

V

P

P

F
x
Y

W

W

A

A

V

A

W

W

P

P

Y

F

E

E

T

T

M

L

I

L

S

P

K

K

R

A

H

H

V

V

N

L

S

R

I

I

R

T

L

D

F

L

T

T

E

D

A

A

E

Q

K

R

E

S

S

D

L

L

A

A

E

L

A

A

I

L

K

K

V

K

L

L

T

T

T

S

K

R

Y

Y

D

D

N

N

L

L

F

F

E

Q

T

C

S

S

F

F

P

P

Y

Y

S

S

A

M

G

G

M

W

H
|
H

Q

G

A

A

P

P

V

F

N

N

N

G

G

E

Y

E

Y

N

P

Q

E

H

W

W

H

Q

W

L

H
|
H

M

A

H
|
H

F

F

Y

Y

P

P

L

L

R

R

S

S

A

A

S

T

V

V

K

R

K

K

F

F

M

M

V

V

G

G

Y

Y

E

E

M

M

L

L

A

A

N

E

P

T

Q

Q

R

R

D

D

I

L

T

T

P

A

E

E

F

Q

A

A

N

E

R

R

L

L

K

R

E

A

M

V

S

S

A

D

T

I

H

H

Y

F

K

R

I

E

S

S

active site: C35 (= C53), C38 (= C56), H98 (= H116), N136 (= N156), S144 (= S164), H147 (= H167), H149 (= H169), Q151 (= Q171)
binding beta-mercaptoethanol: H10 (= H12), N136 (= N156), C143 (= C163), Q151 (= Q171), Y208 (≠ F228)
binding fe (iii) ion: E165 (= E185), H264 (= H284), H279 (= H299), H281 (= H301)
binding uridine-5'-diphosphate: R43 (= R61), V44 (≠ A62), F58 (= F76), N60 (= N78), D61 (= D79), S144 (= S164), N145 (= N165)
binding zinc ion: C35 (= C53), C38 (= C56), H98 (= H116), H147 (= H167)

Sites not aligning to the query:

binding beta-mercaptoethanol: 3, 5

6gqdA Structure of human galactose-1-phosphate uridylyltransferase (galt), with crystallization epitope mutations a21y:a22t:t23p:r25l
49% identity, 98% coverage: 5:348/351 of query aligns to 4:344/344 of 6gqdA

query
sites
6gqdA

F

F

E

L

L

A

D

N

S

D

N

H

P

Q

H

H

T

I

R

R

L

Y

N

N

I

P

L

L

T

Q

G

D

E

E

W

W

V

V

L

L

V

V

S

S

P

A

H

H

R

R

T

M

K

K

R

R

P

P

W

W

Q

Q

G

G

K

Q

V

L

E

L

D

K

V

T

T

V

P

P

D

-

N

-

R

-

P

-

E

R

Y

H

D

D

P

P

K

L

C
x
N

Y
x
P

L

L

C
|
C

P

P

G

G

N

A

S

I

R

R

A
|
A

D

N

G

G

D

E

S

V

N

N

P

P

E

Q

Y

Y

T

D

E

S

S

T

F

F

V

L

F

F

N

D

N
|
N

D
|
D

F

F

A

P

A

A

L

L

L

Q

E

P

D

D

T

A

P

P

A

S

G

P

N

G

M

P

N

S

E

D

H

H

D

P

L

L

L

F

V

Q

A

A

S

K

N

S

Q

A

R

R

G

G

L

V

C

C

K

K

V

V

I

M

S

C

F

F

S

H

P

P

K

W

H
x
S

H

D

L

V

T

T

L

L

P

P

E

L

M

M

S

S

V

V

K

P

A

E

I

I

T

R

A

A

V

V

N

D

V

A

W

W

Q

A

N

S

E

V

F

T

N

E

S

E

L

L

A

G

E

A

N

Q

N

-

W

-

I

Y

K

P

Y

W

I

V

Q

Q

I

I

F

F

E

E

N
|
N

K

K

G

G

E

A

I

M

M

M

G

G

C

C

S
|
S

N

N

P

P

H
|
H

P

P

H
|
H

G

C

Q
|
Q

I

V

W

W

S

A

Q

S

G

S

D

F

I

L

P

P

L

-

E

D

I

I

A

A

K

Q

E

R

T
x
E

E

E

R

R

-

S

Q

Q

K

Q

S

A

Y

Y

Y

K

A

S

I

Q

H

H

K

G

R

E

S

P

L

L

S

M

D

E

Y

Y

L

S

K

R

L

Q

E

E

Q

L

K

L

K

R

K

K

E

E

R

R

I

L

I

V

F

L

E

T

N

S

D

E

H

H

F

W

A

L

V

V

L

L

V

V

P

P

F

F

W

W

A

A

V

T

W

W

P

P

Y

Y

E

Q

T

T

M

L

I

L

S

P

K

R

R

R

H

H

V

V

N

R

S

R

I

L

R

P

L

E

F

L

T

T

E

P

A

A

E

E

K

R

E

D

S

D

L

L

A

A

E

S

A

I

I

M

K

K

V

K

L

L

T

L

T

T

K

K

Y

Y

D

D

N

N

L

L

F

F

E

E

T

T

S

S

F

F

P

P

Y

Y

S

S

A

M

G

G

M

W

H
|
H

Q

G

A

A

P

P

V

T

N

G

N

S

G

E

Y

A

Y

G

P

A

E

N

W

W

-

D

H

H

W

W

-

Q

-

L

H
|
H

M

A

H
|
H

F

Y

Y

Y

P

P

L

L

R

R

S

S

A

A

S

T

V

V

K

R

K

K

F

F

M

M

V

V

G

G

Y

Y

E

E

M

M

L

L

A

A

N

Q

P

A

Q

Q

R

R

D

D

I

L

T

T

P

P

E

E

F

Q

A

A

N

E

R

R

L

L

K

R

E

A

M

L

S

P

A

E

T

V

H

H

Y

Y

K

H

I

L

active site: N48 (≠ C53), C51 (= C56), S111 (≠ H116), N149 (= N156), S157 (= S164), H160 (= H167), H162 (= H169), Q164 (= Q171)
binding 5,6-dihydrouridine-5'-monophosphate: P49 (≠ Y54), A57 (= A62), N73 (= N78), D74 (= D79), H162 (= H169), Q164 (= Q171)
binding zinc ion: E178 (≠ T186), H277 (= H284), H295 (= H299), H297 (= H301)

5in3A Crystal structure of glucose-1-phosphate bound nucleotidylated human galactose-1-phosphate uridylyltransferase (see paper)
49% identity, 95% coverage: 12:346/351 of query aligns to 4:324/324 of 5in3A

query
sites
5in3A

H

H

T

I

R

R

L

Y

N

N

I

P

L

L

T

Q

G

D

E

E

W

W

V

V

L

L

V

V

S

S

P

A

H

H

R

R

T

T

K

V

R

-

P

-

W

-

Q

-

G

-

K

-

V

-

E

-

D

-

V

-

T

-

P

-

D

-

N

-

R

-

P

P

E

R

Y

H

D

D

P

P

K

L

C
x
N

Y
x
P

L

L

C
|
C

P

P

G

G

N

A

S

I

R

R

A
|
A

D

N

G

G

D

E

S

V

N

N

P

P

E

Q

Y

Y

T

D

E

S

S

T

F

F

V

L

F
|
F

N

D

N
|
N

D
|
D

F

F

A

P

A

A

L

L

L

Q

E

P

D

D

T

A

P

P

A

S

G

P

N

G

M

P

N

S

E

D

H

H

D

P

L

L

L

F

V

Q

A

A

S

K

N

S

Q

A

R

R

G

G

L

V

C

C

K

K

V
|
V

I

M

S

C

F

F

S

H

P

P

K

W

H
x
S

H

D

L

V

T

T

L

L

P

P

E

L

M

M

S

S

V

V

K

P

A

E

I

I

T

R

A

A

V

V

N

D

V

A

W

W

Q

A

N

S

E

V

F

T

N

E

S

E

L

L

A

G

E

A

N

Q

N

-

W

-

I

Y

K

P

Y

W

I

V

Q

Q

I

I

F
|
F

E

E

N
|
N

K

K

G

G

E

A

I

M

M

M

G

G

C

C

S
|
S

N

N

P

P

H
|
H

P

P

H
|
H

G

C

Q
|
Q

I

V

W

W

S

A

Q

S

G

S

D

F

I

L

P

P

L

-

E

D

I

I

A

A

K

Q

E

R

T
x
E

E

E

R

R

-

S

Q

Q

K

Q

S

A

Y

Y

Y

K

A

S

I

Q

H

H

K

G

R

E

S

P

L

L

S

M

D

E

Y

Y

L

S

K

R

L

Q

E

E

Q

L

K

L

K

R

K

K

E

E

R

R

I

L

I

V

F

L

E

T

N

S

D

E

H

H

F

W

A

L

V

V

L

L

V

V

P

P

F

F

W

W

A

A

V

T

W

W

P

P

Y

Y

E

Q

T

T

M

L

I

L

S

P

K

R

R

R

H

H

V

V

N

R

S

R

I

L

R

P

L

E

F

L

T

T

E

P

A

A

E

E

K

R

E

D

S

D

L

L

A

A

E

S

A

I

I

M

K

K

V

K

L

L

T

L

T

T

K

K

Y

Y

D

D

N

N

L

L

F

F

E

E

T

T

S

S

F

F

P

P

Y

Y

S

S

A

M

G

G

M

W

H
|
H

Q

G

A

A

P

P

V

T

N

G

N

S

G

E

Y

A

Y

G

P

A

E

N

W

W

-

N

H

H

W

W

-

Q

-

L

H
|
H

M

A

H
|
H

F

Y

Y

Y

P

P

L

L

R

R

S

S

A

A

S

T

V

V

K

R

K

K

F

F

M

M

V
|
V

G
|
G

Y

Y

E
|
E

M

M

L

L

A

A

N

Q

P

A

Q

Q

R

R

D

D

I

L

T

T

P

P

E

E

F

Q

A

A

N

E

R

R

L

L

K

R

E

A

M

L

S

P

A

E

T

V

H

H

Y

Y

active site: N30 (≠ C53), C33 (= C56), S93 (≠ H116), N131 (= N156), S139 (= S164), H142 (= H167), H144 (= H169), Q146 (= Q171)
binding 1-O-phosphono-alpha-D-glucopyranose: F129 (= F154), N131 (= N156), Q146 (= Q171), V295 (= V317), G296 (= G318), E298 (= E320)
binding 5,6-dihydrouridine-5'-monophosphate: P31 (≠ Y54), A39 (= A62), F53 (= F76), N55 (= N78), D56 (= D79), V86 (= V109), H144 (= H169), Q146 (= Q171)
binding zinc ion: E160 (≠ T186), H259 (= H284), H277 (= H299), H279 (= H301)

6k5zB Structure of uridylyltransferase (see paper)
30% identity, 93% coverage: 14:339/351 of query aligns to 4:306/314 of 6k5zB

query
sites
6k5zB

R

R

L

K

N

D

I

P

L

F

T

T

G

G

E

E

W

Y

V

I

L

L

V

V

S

S

P

P

H

H

R

R

T

L

K

K

R

R

P

P

W

W

Q

Q

G

-

K

-

V

-

E

-

D

-

V

-

T

-

P

P

D

E

N

G

R

-

P

-

E

-

Y

-

D

-

P

-

K

A

C
|
C

Y

P

L

F

C
|
C

P

P

G

G

N

A

S

P

R

E

A

T

D

G

G

R

D

G

S

W

N

D

P

-

E

-

Y

-

T

-

E

-

S

V

F

L

V

I

F

L

N

P

N

N

D

R

F

Y

A

P

A

V

L

V

L

T

E

E

D

N

T

P

P

P

A

E

G

P

N

T

M

A

N

E

E

-

H

-

D

D

L

L

L

Y

V

E

A

V

S

I

N

P

Q

A

R

R

G

G

L

S

C

S

K

L

V

V

I

V

S

V

F

E

S

T

P

P

K

Q

H
|
H

H

D

L

V

T

D

-

D

-

L

-

S

-

D

L

L

P

P

E

L

M

G

S

Q

V

I

K

K

A

K

I

I

T

L

A

T

V

A

V

V

N

A

V

E

W

A

Q

Q

N

R

E

K

F

A

N

E

S

K

L

-

A

-

E

E

N

G

N

N

W

-

I

A

K

A

Y

Y

I

F

Q

L

I

F

F

F

E

R

N
|
N

K

K

G

G

E

K

I

E

M

I

G

G

C
x
V

S
|
S

N
x
L

P

T

H
|
H

P

P

H
|
H

G

S

Q
|
Q

I

I

W

Y

S

I

Q

L

G

P

D

V

I

V

P

P

L

P

E

R

I

V

A

R

K

A

E
|
E

T

L

E

Q

R

A

Q

S

K

Y

S

E

Y

W

Y

Y

A

V

I

K

H

H

K

G

R

S

S
x
C

L

L

L

-

S

-

D

-

Y

H

L
x
C

K

R

L

I

E

V

Q

E

K

K

K

E

E

K

R

R

I

L

I

V

F

F

E

Q

N

N

D

R

H

N

F

W

A

K

V

A

L

F

V

V

P

P

F

F

W

Y

A

A

V

K

W

W

P

P

Y

H

E

E

T

V

M

H

I

I

I

Y

S

P

K

K

R

R

H
|
H

V

R

N

S

S

L

I

L

R

T

L

E

F

L

T

T

E

D

A

E

E

E

K

V

E

A

S

D

L

L

A

A

E

E

A

A

I

L

K

K

V

I

L

T

T

L

T

C

K

A

Y

L

D

K

N

Q

L

V

F

A

E

G

T

I

S

P

F

M

P

P

Y

Y

S

I

A

M

G

V

M

L

H
|
H

Q

Q

A

A

P

P

V

L

N

P

N

R

G

-

Y

-

P

P

E

T

W

Q

H

Y

W

Y

H
|
H

M

L

H
|
H

F

F

-
x
E

Y

I

P

Y

P

G

L

M

L

Y

R

R

S

P

A

D

S

G

V

K

K

L

K

K

F

H

M

A

V

A

G

G

Y

A

E

E

M

L

L

G

A

A

N

S

P

L

-

F

Q

T

R

L

D

D

I

T

T

T

P

P

E

E

F

E

A

T

A

A

N

A

R

R

L

I

K

K

active site: C30 (= C53), C33 (= C56), H86 (= H116), N127 (= N156), S135 (= S164), H138 (= H167), H140 (= H169), Q142 (= Q171)
binding fe (iii) ion: E156 (= E185), H252 (= H284), H266 (= H301), E268 (vs. gap)
binding phosphate ion: V134 (≠ C163), S135 (= S164), L136 (≠ N165), H140 (= H169)
binding zinc ion: C30 (= C53), C33 (= C56), H86 (= H116), H138 (= H167), C170 (≠ S199), C173 (≠ L205), H211 (= H243), H264 (= H299)

Q9FK51 ADP-glucose phosphorylase; ADP-glucose:phosphate adenylyltransferase; EC 2.7.7.- from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
26% identity, 96% coverage: 8:344/351 of query aligns to 20:351/351 of Q9FK51

query
sites
Q9FK51

D

N

S

Q

N

S

P

P

H

E

T

L

R

R

L

K

N

D

I

P

L

V

T

T

G

N

E

R

W

W

V

V

L

I

V

F

S

S

P

P

H

A

R
|
R

T
x
A

K
|
K

R
|
R

P

P

W

T

Q

D

G

F

K

K

V

S

E

K

D

-

V

-

T

S

P

P

D

Q

N

N

R

P

P

N

E

P

Y

K

D

P

P

S

K

S

C
|
C

Y

P

L

F

C
|
C

P

I

G

G

N

R

S

E

R

Q

A
x
E

D
x
C

G
x
A

-

P

-

E

-

L

-

F

-

R

-

V

-

P

D

D

S

H

N

D

P

P

E

N

Y

W

T

-

E

K

S

L

F

R

V

V

F

I

N

E

N
|
N

D

L

F

Y

A

P

A

A

L

L

L

S

E

R

D

N

T

L

P

E

A

T

G

Q

N

S

M

T

N

Q

-

P

E

E

H

T

D

G

L

T

L

S

V

R

A

T

S

I

N

V

Q

G

R

F

G

G

L

F

C

H

K

D

V

V

I

V

S

I

F

E

S

S

P

P

K

V

H
|
H

H

S

L

I

T

Q

L

L

P

S

E

D

M

I

S

D

V

P

K

V

A

G

I

I

T

G

A

D

V

I

V

L

N

I

V

A

W

Y

Q

K

N

K

E

R

F

I

N

N

S

Q

L

I

A

A

E

Q

N

H

N

D

W

S

I

I

K

N

Y

Y

I

I

Q

Q

I

V

F

F

E

K

N
|
N

K

Q

G

G

E

A

I

S

M

A

G
|
G

C
x
A

S
|
S

N
x
M

P

S

H
|
H

P

S

H
|
H

G

S

Q
|
Q

I

M

W

M

S

A

Q

L

G

P

D

V

I

V

P

P

L

P

E

T

I

V

A

S

K

S

E

R

T

L

E

D

R

G

Q

T

K

K

S

D

Y

Y

Y

F

A

E

I

E

H

T

K

G

R

K

S
x
C

L

C

L

L

S

-

D

-

Y

-

L

-

K

-

L
x
C

E

E

Q

A

K

K

K

S

K

K

E

H

R

F

I

V

I

I

F

D

E

E

N

S

D

S

H

H

F

F

A

V

V

S

L

V

V

A

P

P

F

F

W

A

A

A

V

T

W

Y

P

P

Y

F

E

E

T

I

M

W

I

I

S

P

K

K

R

D

H
|
H

V

S

N

S

S

H

I

F

R

H

L

H

F

L

T

D

E

D

A

V

E

K

K

A

E

V

S

D

L

L

A

G

E

G

A

L

I

L

K

K

V

L

L

M

T

L

T

Q

K

K

Y

I

D

A

N

K

L

Q

F

L

E

N

T

D

S

P

F

-

P

P

Y

Y

S

N

A

Y

G

M

M

I

H

H

Q

T

A

S

P

P

-

L

-

K

V

V

N

T

N

E

G

S

Y

Q

Y

L

P

P

E

Y

W

T

H
|
H

W

W

H

F

M

L

H

Q

F

I

Y

V

P

P

P

Q

L

L

L

-

R

-

S

-

A

S

S
x
G

V

V

K

G

F
|
F

M
x
E

V

I

G

G

Y

T

E

G

M

C

L

Y

A

I

N

N

P

P

Q

-

R

-

D

-

I

V

T

F

P

P

E

E

F

D

A

V

A

A

N

K

R

V

L

M

K

R

E

E

M

V

S

S

A

L

T

T

RAKR 41:44 (≠ RTKR 29:32) binding
C63 (= C53) binding
C66 (= C56) binding
ECA 72:74 (≠ ADG 62:64) binding
N94 (= N78) binding
H133 (= H116) binding
N173 (= N156) binding
GASM 179:182 (≠ GCSN 162:165) binding
H184 (= H167) binding
H186 (= H169) active site, Tele-AMP-histidine intermediate
Q188 (= Q171) binding
C216 (≠ S199) binding
C219 (≠ L207) binding
H255 (= H243) binding
H310 (= H297) binding
G321 (≠ S311) binding
FE 325:326 (≠ FM 315:316) binding

6k9zA Structure of uridylyltransferase mutant (see paper)
28% identity, 93% coverage: 14:339/351 of query aligns to 4:299/309 of 6k9zA

query
sites
6k9zA

R

R

L

K

N

D

I

P

L

F

T

T

G

G

E

E

W

Y

V

I

L

L

V

V

S

S

P

P

H

-

R

-

T

-

K

-

R

-

P

-

W

-

Q

-

G

-

K

-

V

-

E

-

D

-

V

-

T

-

P

-

D

-

N

-

R

Q

P

P

E

E

Y

G

D

-

P

-

K

A

C
|
C

Y
x
P

L

F

C
|
C

P

P

G

G

N

A

S

P

R

E

A

T

D

G

G

R

D

G

S

W

N

D

P

-

E

-

Y

-

T

-

E

-

S

V

F

L

V

I

F

L

N

P

N
|
N

D
x
R

F
x
Y

A

P

A

V

L

V

L

T

E

E

D

N

T

P

P

P

A

-

G

-

N

E

M

P

N

T

E

A

H

E

D

D

L

L

L

Y

V

E

A

V

S

I

N

P

Q

A

R

R

G

G

L

S

C

S

K

L

V

V

I

V

S

V

F

E

S

T

P

P

K

Q

H
|
H

H

D

L

V

T

D

-

D

-

L

-

S

-

D

L

L

P

P

E

L

M

G

S

Q

V

I

K

K

A

K

I

I

T

L

A

T

V

A

V

V

N

A

V

E

W

A

Q

Q

N

R

E

K

F

A

N

E

S

K

L

-

A

-

E

E

N

G

N

N

W

-

I

A

K

A

Y

Y

I

F

Q

L

I

F

F

F

E

R

N
|
N

K

K

G

G

E

K

I

E

M

I

G

G

C

V

S
|
S

N
x
L

P

T

H
|
H

P

P

H
x
F

G

S

Q
|
Q

I

I

W
x
Y

S

I

Q

L

G

P

D

V

I

V

P

P

L

P

E

R

I

V

A

R

K

A

E
|
E

T

L

E

Q

R

A

Q

S

K

Y

S

E

Y

W

Y

Y

A

V

I

K

H

H

K

G

R

S

S
x
C

L

L

L

-

S

-

D

-

Y

H

L
x
C

K

R

L

I

E

V

Q

E

K

K

K

E

E

K

R

R

I

L

I

V

F

F

E

Q

N

N

D

R

H

N

F

W

A

K

V

A

L

F

V

V

P

P

F

F

W

Y

A

A

V

K

W

W

P

P

Y

H

E

E

T

V

M

H

I

I

I

Y

S

P

K

K

R

R

H
|
H

V

R

N

S

S

L

I

L

R

T

L

E

F

L

T

T

E

D

A

E

E

E

K

V

E

A

S

D

L

L

A

A

E

E

A

A

I

L

K

K

V

I

L

T

T

L

T

C

K

A

Y

L

D

K

N

Q

L

V

F

A

E

G

T

I

S

P

F

M

P

P

Y

Y

S

I

A

M

G

V

M

L

H
|
H

Q

Q

A

A

P

P

V

L

N

P

N

R

G

-

Y

-

P

P

E

T

W

Q

H

Y

W

Y

H
|
H

M

L

H
|
H

F

F

-
x
E

Y

I

P

Y

P

G

L

M

L

Y

R

R

S

P

A

D

S

G

V

K

K

L

K

K

F

H

M

A

V

A

G

G

Y

A

E

E

M

L

L

G

A

A

N

S

P

L

-

F

Q

T

R

L

D

D

I

T

T

T

P

P

E

E

F

E

A

T

A

A

N

A

R

R

L

I

K

K

active site: C23 (= C53), C26 (= C56), H79 (= H116), N120 (= N156), S128 (= S164), H131 (= H167), F133 (≠ H169), Q135 (= Q171)
binding fe (iii) ion: E149 (= E185), H245 (= H284), H259 (= H301), E261 (vs. gap)
binding uridine-5'-diphosphate: P24 (≠ Y54), N43 (= N78), R44 (≠ D79), Y45 (≠ F80), L129 (≠ N165), Q135 (= Q171), Y137 (≠ W173)
binding zinc ion: C23 (= C53), C26 (= C56), H79 (= H116), H131 (= H167), C163 (≠ S199), C166 (≠ L205), H204 (= H243), H257 (= H299)

2h39B Crystal structure of an adp-glucose phosphorylase from arabidopsis thaliana with bound adp-glucose
25% identity, 95% coverage: 10:344/351 of query aligns to 2:313/313 of 2h39B

query
sites
2h39B

N

S

P

P

H

E

T

L

R

R

L

K

N

D

I

P

L

V

T

T

G

N

E

R

W

W

V

V

L

I

V

F

S

S

P

P

H

A

R
|
R

T

A

K

K

R
|
R

P

P

W

T

Q

-

G

-

K

-

V

-

E

-

D

-

V

-

T

-

P

-

D

-

N

-

R

-

P

-

E

D

Y

F

D

P

P

S

K

S

C
|
C

Y

P

L
x
F

C
|
C

P

I

G

G

N

R

S

E

R

Q

A

E

D
x
C

G

A

-

P

-

E

-

L

-

F

-

R

-

V

-

P

D

D

S

H

N

D

P

P

E

N

Y

W

T

K

E

L

S

R

F

-

V

V

F

I

N

E

N
|
N

D
x
L

F
x
Y

A

P

A

A

L

L

L

-

E

-

D

-

T

-

P

-

A

S

G

R

N

N

M

L

N

E

E

T

H

Q

D

S

L

T

L

R

V

T

A

I

S

V

N

G

Q

-

R

F

G

G

L

F

C

H

K

D

V

V

I

V

S

I

F

E

S

S

P

P

K

V

H
|
H

H

S

L

I

T

Q

L

L

P

S

E

D

M

I

S

D

V

P

K

V

A

G

I

I

T

G

A

D

V

I

V

L

N

I

V

A

W

Y

Q

K

N

K

E

R

F

I

N

N

S

Q

L

I

A

A

E

Q

N

H

N

D

W

S

I

I

K

N

Y

Y

I

I

Q

Q

I

V

F
|
F

E

K

N
|
N

K

Q

G

G

E

A

I

S

M

A

G
|
G

C
x
A

S
|
S

N
x
M

P

S

H
|
H

P

S

H
x
G

G

S

Q
|
Q

I

M

W

M

S

A

Q

L

G

P

D

V

I

V

P

P

L

P

E

T

I

V

A

S

K

S

E

R

T

L

E

D

R

G

Q

T

K

K

S

D

Y

Y

Y

F

A

E

I

E

H

T

K

G

R

K

S
x
C

L

C

L

L

S
x
C

D

-

Y

-

L

-

K

-

L

-

E

E

Q

A

K

K

K

S

K

K

E

H

R

F

I

V

I

I

F

D

E

E

N

S

D

S

H

H

F

F

A

V

V

S

L

V

V

A

P

P

F

F

W

A

A

A

V

T

W

Y

P

P

Y

F

E

E

T

I

M

W

I

I

S

P

K

K

R

D

H
|
H

V

S

N

S

S

H

I

F

R

H

L

H

F

L

T

D

E

D

A

V

E

K

K

A

E

V

S

D

L

L

A

G

E

G

A

L

I

L

K

K

V

L

L

M

T

L

T

Q

K

K

Y

I

D

A

N

K

L

Q

F

L

E

N

T

D

S

P

F

-

P

P

Y

Y

S

N

A

Y

G

M

M

I

H

H

Q

T

A

S

P

P

-

L

-

K

V

V

N

T

N

E

G

S

Y

Q

Y

L

P

P

E

Y

W

T

H
|
H

W

W

H

F

M

L

H

Q

F

I

Y

V

P

P

P

Q

L

L

L

-

R

-

S

-

A

S

S

G

V

V

K
x
G

K

G

F
|
F

M
x
E

V

I

G

G

Y

T

E

G

M

C

L

Y

A

I

N

N

P

P

Q

-

R

-

D

-

I

V

T

F

P

P

E

E

F

D

A

V

A

A

N

K

R

V

L

M

K

R

E

E

M

V

S

S

A

L

T

T

active site: C32 (= C53), C35 (= C56), H95 (= H116), N135 (= N156), S143 (= S164), H146 (= H167), G148 (≠ H169), Q150 (= Q171)
binding adenosine-5'-diphosphate-glucose: R21 (= R29), R24 (= R32), F34 (≠ L55), C42 (≠ D63), N63 (= N78), L64 (≠ D79), Y65 (≠ F80), F133 (= F154), N135 (= N156), G141 (= G162), A142 (≠ C163), S143 (= S164), M144 (≠ N165), Q150 (= Q171), G285 (≠ K313), F287 (= F315), E288 (≠ M316)
binding zinc ion: C32 (= C53), H95 (= H116), H146 (= H167), C178 (≠ S199), C181 (≠ S202), H217 (= H243), H272 (= H297)

1z84A X-ray structure of galt-like protein from arabidopsis thaliana at5g18200 (see paper)
24% identity, 95% coverage: 10:344/351 of query aligns to 1:311/311 of 1z84A

query
sites
1z84A

N

S

P

P

H

E

T

L

R

R

L

K

N

D

I

P

L

V

T

T

G

N

E

R

W

W

V

V

L

I

V

F

S

S

P

P

H

R

R

P

T

T

K

-

R

-

P

-

W

-

Q

-

G

-

K

-

V

-

E

-

D

-

V

-

T

-

P

-

D

D

N

F

R

K

P

S

E

K

Y

S

D

P

P

S

K

S

C
|
C

Y

P

L
x
F

C
|
C

P

I

G

G

N

R

S

E

R

Q

A

E

D

C

G

A

-

P

-

E

-

L

-

F

-

R

-

V

-

P

D

D

S

H

N

D

P

P

E

N

Y

W

T

K

E

L

S

R

F

-

V

V

F

I

N

E

N
|
N

D
x
L

F
x
Y

A

P

A

A

L

L

L

-

E

-

D

-

T

-

P

-

A

S

G

R

N

N

M

L

N

E

E

T

H

Q

D

S

L

R

L

T

V

I

A

V

S

G

N

-

Q

-

R

F

G

G

L

F

C

H

K

D

V

V

I

V

S

I

F

E

S

S

P

P

K

V

H
|
H

H

S

L

I

T

Q

L

L

P

S

E

D

M

I

S

D

V

P

K

V

A

G

I

I

T

G

A

D

V

I

V

L

N

I

V

A

W

Y

Q

K

N

K

E

R

F

I

N

N

S

Q

L

I

A

A

E

Q

N

H

N

D

W

S

I

I

K

N

Y

Y

I

I

Q

Q

I

V

F

F

E

K

N
|
N

K

Q

G

G

E

A

I

S

M

A

G

G

C
x
A

S
|
S

N
x
M

P

S

H
|
H

P

S

H
|
H

G

S

Q
|
Q

I

M

W

M

S

A

Q

L

G

P

D

V

I

V

P

P

L

P

E

T

I

V

A

S

K

S

E

R

T

L

E

D

R

G

Q

T

K

K

S

D

Y

Y

Y

F

A

E

I

E

H

T

K

G

R

K

S
x
C

L

C

L

L

S
x
C

D

-

Y

-

L

-

K

-

L

-

E

E

Q

A

K

K

K

S

K

K

E

H

R

F

I

V

I

I

F

D

E

E

N

S

D

S

H

H

F

F

A

V

V

S

L

V

V

A

P

P

F

F

W

A

A

A

V

T

W

Y

P

P

Y

F

E

E

T

I

M

W

I

I

S

P

K

K

R

D

H
|
H

V

S

N

S

S

H

I

F

R

H

L

H

F

L

T

D

E

D

A

V

E

K

K

A

E

V

S

D

L

L

A

G

E

G

A

L

I

L

K

K

V

L

L

M

T

L

T

Q

K

K

Y

I

D

A

N

K

L

Q

F

L

E

N

T

D

S

P

F

-

P

P

Y

Y

S

N

A

Y

G

M

M

I

H

H

Q

T

A

S

P

P

-

L

-

K

V

V

N

T

N

E

G

S

Y

Q

Y

L

P

P

E

Y

W

T

H
|
H

W

W

H

F

M

L

H

Q

F

I

Y

V

P

P

P

Q

L

L

L

-

R

-

S

-

A

S

S

G

V

V

K

G

F

F

M

E

V

I

G

G

Y

T

E

G

M

C

L

Y

A

I

N

N

P

P

Q

-

R

-

D

-

I

V

T

F

P

P

E

E

F

D

A

V

A

A

N

K

R

V

L

M

K

R

E

E

M

V

S

S

A

L

T

T

active site: C31 (= C53), C34 (= C56), H93 (= H116), N133 (= N156), S141 (= S164), H144 (= H167), H146 (= H169), Q148 (= Q171)
binding adenosine monophosphate: F33 (≠ L55), N62 (= N78), L63 (≠ D79), Y64 (≠ F80), N133 (= N156), A140 (≠ C163), S141 (= S164), M142 (≠ N165), H146 (= H169), Q148 (= Q171)
binding zinc ion: C31 (= C53), C34 (= C56), H93 (= H116), H144 (= H167), C176 (≠ S199), C179 (≠ S202), H215 (= H243), H270 (= H297)

Query Sequence

>CA265_RS06120 FitnessBrowser__Pedo557:CA265_RS06120
MNQTFELDSNPHTRLNILTGEWVLVSPHRTKRPWQGKVEDVTPDNRPEYDPKCYLCPGNS
RADGDSNPEYTESFVFNNDFAALLEDTPAGNMNEHDLLVASNQRGLCKVISFSPKHHLTL
PEMSVKAITAVVNVWQNEFNSLAENNWIKYIQIFENKGEIMGCSNPHPHGQIWSQGDIPL
EIAKETERQKSYYAIHKRSLLSDYLKLEQKKKERIIFENDHFAVLVPFWAVWPYETMIIS
KRHVNSIRLFTEAEKESLAEAIKVLTTKYDNLFETSFPYSAGMHQAPVNNGYYPEWHWHM
HFYPPLLRSASVKKFMVGYEMLANPQRDITPEFAANRLKEMSATHYKISKV

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory