SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing CA265_RS06310 FitnessBrowser__Pedo557:CA265_RS06310 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 7 hits to proteins with known functional sites (download)

Q29551 Succinyl-CoA:3-ketoacid coenzyme A transferase 1, mitochondrial; SCOT; 3-oxoacid CoA-transferase 1; Somatic-type succinyl-CoA:3-oxoacid CoA-transferase; SCOT-s; Succinate-coenzyme A transferase; Succinyl-CoA:3-oxoacid CoA transferase; EC 2.8.3.5 from Sus scrofa (Pig) (see 3 papers)
56% identity, 91% coverage: 4:203/219 of query aligns to 302:502/520 of Q29551

query
sites
Q29551

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E344 (= E46) active site, 5-glutamyl coenzyme A thioester intermediate

Sites not aligning to the query:

1:39 modified: transit peptide, Mitochondrion
67 C→A: Decreases KM for acetoacetyl-CoA 3-fold. No effect on KM for acetoacetate, succinyl-CoA and succinate.; C→S: Decreases KM for acetoacetate and acetoacetyl-CoA 2-fold. Increases KM for succinate 2-fold. Decreases KM for succinyl-CoA nearly 4-fold. Strongly reduces catalytic activity.
235 C→S: Similar specific activity to wild-type.

3k6mC Dynamic domains of succinyl-coa:3-ketoacid-coenzyme a transferase from pig heart. (see paper)
56% identity, 91% coverage: 4:203/219 of query aligns to 249:449/466 of 3k6mC

query
sites
3k6mC

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binding glycerol: I270 (= I25), E291 (= E46), A313 (= A68), V377 (= V132), A380 (= A135), T382 (≠ N137), C412 (= C166), N414 (≠ K168)

Sites not aligning to the query:

binding glycerol: 25, 52, 76, 99

1o9lA Succinate:coenzyme-a transferase (pig heart)
56% identity, 91% coverage: 4:203/219 of query aligns to 250:450/468 of 1o9lA

query
sites
1o9lA

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Sites not aligning to the query:

binding 2-(ethylmercuri-thio)-benzoic acid: 87, 94, 95, 96, 131, 132, 145

3oxoE Succinyl-coa:3-ketoacid coa transferase from pig heart covalently bound to coa (see paper)
55% identity, 92% coverage: 2:203/219 of query aligns to 243:445/462 of 3oxoE

query
sites
3oxoE

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binding coenzyme a: I266 (= I25), G267 (= G26), I268 (= I27), E287 (= E46), G343 (= G102), M345 (= M104), N355 (= N114), I358 (= I117), M366 (= M125), A369 (= A128), K389 (= K147)

8i3yD Crystal structure of asct from trypanosoma brucei in complex with succinyl-coa.
56% identity, 91% coverage: 4:203/219 of query aligns to 257:455/471 of 8i3yD

query
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8i3yD

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Sites not aligning to the query:

binding calcium ion: 201, 229

8i40A Crystal structure of asct from trypanosoma brucei in complex with coa.
56% identity, 91% coverage: 4:203/219 of query aligns to 253:451/467 of 8i40A

query
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8i40A

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T

T

binding coenzyme a: I274 (= I25), I276 (= I27), E295 (= E46), G351 (= G102), L353 (≠ M104), N363 (= N114), G376 (= G127), K401 (= K152)

8i3yA Crystal structure of asct from trypanosoma brucei in complex with succinyl-coa.
56% identity, 91% coverage: 5:203/219 of query aligns to 248:445/459 of 8i3yA

query
sites
8i3yA

E

R

I

I

A

A

Q

R

R

R

I

A

A

A

K

L

E

E

I

F

K

A

D

N

G

G

Y

M

Y

Y

V

V

N

N

L

L

G
|
G

I
|
I

G
|
G

I

I

P

P

T

T

L

E

V

S

A

S

N

N

Y

Y

I

I

P

P

K

A

G

G

I

V

N

N

V

V

L

L

Q

Q

S

S

E
|
E

N

N

G

G

L

L

L

I

G

G

M

M

G

G

P

P

F

F

P

P

F

T

E

E

G

D

E

K

E

V

D

D

A

A

D

D

L

W

I

I

N

N

A
|
A

G

G

K
|
K

Q

Q

T

T

I

I

T

S

T

H

L

L

P

A

G

G

S

S

S

A

I

L

F

F

D

D

S

S

A

A

M

T

S

S

F

F

G

A

M

M

I

I

R

R

A

G

Q

G

K

H

V

M

D

D

L

L

T

T

I

M

L

L

G

G

A

A

M

L

E

E

V

V

S

A

E

A

N

N

G

G

D

D

I

L

A

A

N
|
N

W

F

K

M

I

I

P

P

G

G

K

K

M

L

V

V

K

K

G

G

M

P

G
|
G

A
|
A

M

M

D

D

L

L

V

V

A

S

S

C

A

G

K

T

N

R

I

V

V

V

A

T

M

T

Q

T

H

H

I

C

N
|
N

K
|
K

A

N

G

G

E

D

S

P

K
|
K

L

I

L

V

P

E

K

R

C

C

T

R

L

L

P

P

L

V

T

T

G

G

V

K

K

H

C

C

I

V

K

C

K

R

I

I

V

I

T

T

E

E

L

Y

A

A

V

V

L

F

D

D

I

V

L

V

P

-

E

D

G

G

G

R

F

L

K

V

L

L

L

K

E

E

R

I

A

A

P

E

G

D

V

T

S

T

I

V

E

D

F

Q

I

V

Q

K

S

L

T

T

binding succinyl-coenzyme a: G267 (= G24), I268 (= I25), G269 (= G26), E289 (= E46), A311 (= A68), K313 (= K70), N357 (= N114), G369 (= G126), G370 (= G127), A371 (= A128), N389 (= N146), K390 (= K147), K395 (= K152)

Sites not aligning to the query:

Query Sequence

>CA265_RS06310 FitnessBrowser__Pedo557:CA265_RS06310
MFSKEEIAQRIAKEIKDGYYVNLGIGIPTLVANYIPKGINVVLQSENGLLGMGPFPFEGE
EDADLINAGKQTITTLPGSSIFDSAMSFGMIRAQKVDLTILGAMEVSENGDIANWKIPGK
MVKGMGGAMDLVASAKNIIVAMQHINKAGESKLLPKCTLPLTGVKCIKKIVTELAVLDIL
PEGGFKLLERAPGVSIEFIQQSTAGRLIIEGDIPEMRLD

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory