SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing CA265_RS08605 FitnessBrowser__Pedo557:CA265_RS08605 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

4gvxA Crystal structure of a short chain dehydrogenase homolog (target efi- 505321) from burkholderia multivorans, with bound NADP and l-fucose
53% identity, 99% coverage: 1:259/262 of query aligns to 1:257/258 of 4gvxA

query
sites
4gvxA

M

M

D

D

L

L

Q

N

L

L

K

Q

E

D

K

K

V

V

I

V

V

I

I

V

T

T

G
|
G

A

G

A

A

K
x
S

G
|
G

I
|
I

G

G

R

G

S

A

I

I

A

S

E

M

V

R

F

L

A

A

K

E

E

E

N

R

A

A

I

I

A

P

V

V

I

V

V

F

G

A

R
|
R

K
x
H

A

A

E

P

D

D

N

G

Q

A

I

F

V

L

V

-

D

D

A

A

I

L

A

A

T

Q

N

R

G

Q

G

P

K

R

A

A

A

T

Q

Y

F

L

V

P

A

V

E
|
E

L
|
L

S

Q

N

D

P

D

E

A

D

Q

C

C

E

R

T

D

V

A

V

V

K

A

N

Q

I

T

V

I

A

A

Q

T

F

F

G

G

R

R

I

L

D

D

G

G

L

L

V

V

N

N

N
|
N

A

A

G

G

V

V

N
|
N

D

D

G

G

V

I

G

G

L

L

E

D

S

A

G

G

N

R

Y

-

K

D

D

A

F

F

M

V

A

A

S

S

L

L

H

E

K

R

N

N

V

L

V

I

H

H

Y

Y

L

A

M

M

A

A

H

H

H

Y

V

C

L

V

P

P

E

H

L

L

I

K

K

A

S

T

K

R

G

G

A

A

I

I

V

V

N

N

I
|
I

T
x
S

S
|
S

K
|
K

T

T

A

A

E

V

T

T

G

G

Q
|
Q

G

G

N

N

T
|
T

S

S

A

G

Y
|
Y

A

C

A

A

A

S

N
x
K

G

G

G

A

R

Q

N

L

A

A

L

L

T

T

R

R

E

E

W

W

A

A

V

V

E

A

L

L

L

R

K

E

Y

H

G

G

I

V

R

R

V

V

N

N

A

A

V

V

V

I

V
x
P

A
|
A

E
|
E

C
x
V

W

M

T
|
T

P

P

A
x
L

Y
|
Y

E

R

T

N

W
|
W

I

I

E

A

T

T

L

F

D

E

N

D

A

P

E

E

E

A

K

K

L

L

K

A

E

E

I

I

T

A

A

A

K

K

I

V

P

P

L

L

E

G

N

R

R

R

M

F

T

T

A

P

E

D

E

E

I

I

A

A

N

D

M

T

T

A

A

V

F

F

L

L

M

L

S

S

S

P

K

R

S

A

S

S

H

H

T

T

G

G

Q

E

I

W

I

L

H

F

V

V

D

D

G

G

Y

Y

V

T

H

H

L

L

D

D

R

R

A

A

L

L

active site: G18 (= G18), S140 (= S142), T150 (= T152), Y153 (= Y155), K157 (≠ N159), W194 (= W196)
binding beta-L-fucopyranose: N94 (= N95), S140 (= S142), K141 (= K143), Q147 (= Q149), Y153 (= Y155), A184 (= A186), E185 (= E187), Y191 (= Y193), W194 (= W196)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G14 (= G14), S17 (≠ K17), G18 (= G18), I19 (= I19), R39 (= R39), H40 (≠ K40), E63 (= E64), L64 (= L65), N90 (= N91), I138 (= I140), S139 (≠ T141), S140 (= S142), Y153 (= Y155), K157 (≠ N159), P183 (≠ V185), V186 (≠ C188), T188 (= T190), L190 (≠ A192), Y191 (= Y193)

4gloA Crystal structure of a short chain dehydrogenase homolog (target efi- 505321) from burkholderia multivorans, with bound NAD
53% identity, 99% coverage: 1:259/262 of query aligns to 1:257/258 of 4gloA

query
sites
4gloA

M

M

D

D

L

L

Q

N

L

L

K

Q

E

D

K

K

V

V

I

V

V

I

I

V

T

T

G
|
G

A

G

A

A

K

S

G
|
G

I
|
I

G

G

R

G

S

A

I

I

A

S

E

M

V

R

F

L

A

A

K

E

E

E

N

R

A

A

I

I

A

P

V

V

I

V

V

F

G
x
A

R
|
R

K

H

A

A

E

P

D

D

N

G

Q

A

I

F

V

L

V

-

D

D

A

A

I

L

A

A

T

Q

N

R

G

Q

G

P

K

R

A

A

A

T

Q

Y

F

L

V

P

A
x
V

E
|
E

L
|
L

S

Q

N

D

P

D

E

A

D

Q

C

C

E

R

T

D

V

A

V

V

K

A

N

Q

I

T

V

I

A

A

Q

T

F

F

G

G

R

R

I

L

D

D

G

G

L

L

V

V

N

N

N
|
N

A
|
A

G

G

V

V

N

N

D

D

G

G

V

I

G

G

L

L

E

D

S

A

G

G

N

R

Y

-

K

D

D

A

F

F

M

V

A

A

S

S

L

L

H

E

K

R

N

N

V

L

V

I

H

H

Y

Y

L

A

M

M

A

A

H

H

H

Y

V

C

L

V

P

P

E

H

L

L

I

K

K

A

S

T

K

R

G

G

A

A

I

I

V

V

N

N

I
|
I

T
x
S

S
|
S

K

K

T

T

A

A

E

V

T

T

G

G

Q

Q

G

G

N

N

T
|
T

S

S

A

G

Y
|
Y

A

C

A

A

A

S

N
x
K

G

G

G

A

R

Q

N

L

A

A

L

L

T

T

R

R

E

E

W

W

A

A

V

V

E

A

L

L

L

R

K

E

Y

H

G

G

I

V

R

R

V

V

N

N

A

A

V

V

V

I

V
x
P

A

A

E

E

C
x
V

W

M

T

T

P

P

A
x
L

Y

Y

E

R

T

N

W

W

I

I

E

A

T

T

L

F

D

E

N

D

A

P

E

E

E

A

K

K

L

L

K

A

E

E

I

I

T

A

A

A

K

K

I

V

P

P

L

L

E

G

N

R

R

R

M

F

T

T

A

P

E

D

E

E

I

I

A

A

N

D

M

T

T

A

A

V

F

F

L

L

M

L

S

S

S

P

K

R

S

A

S

S

H

H

T

T

G

G

Q

E

I

W

I

L

H

F

V

V

D

D

G

G

Y

Y

V

T

H

H

L

L

D

D

R

R

A

A

L

L

active site: G18 (= G18), S140 (= S142), T150 (= T152), Y153 (= Y155), K157 (≠ N159)
binding nicotinamide-adenine-dinucleotide: G14 (= G14), I19 (= I19), A38 (≠ G38), R39 (= R39), V62 (≠ A63), E63 (= E64), L64 (= L65), N90 (= N91), A91 (= A92), I138 (= I140), S139 (≠ T141), S140 (= S142), Y153 (= Y155), K157 (≠ N159), P183 (≠ V185), V186 (≠ C188), L190 (≠ A192)

4gkbB Crystal structure of a short chain dehydrogenase homolog (target efi- 505321) from burkholderia multivorans, unliganded structure
53% identity, 99% coverage: 1:259/262 of query aligns to 1:257/258 of 4gkbB

query
sites
4gkbB

M

M

D

D

L

L

Q

N

L

L

K

Q

E

D

K

K

V

V

I

V

V

I

I

V

T

T

G

G

A

G

A

A

K

S

G
|
G

I

I

G

G

R

G

S

A

I

I

A

S

E

M

V

R

F

L

A

A

K

E

E

E

N

R

A

A

I

I

A

P

V

V

I

V

V

F

G

A

R

R

K

H

A

A

E

P

D

D

N

G

Q

A

I

F

V

L

V

-

D

D

A

A

I

L

A

A

T

Q

N

R

G

Q

G

P

K

R

A

A

A

T

Q

Y

F

L

V

P

A

V

E

E

L

L

S

Q

N

D

P

D

E

A

D

Q

C

C

E

R

T

D

V

A

V

V

K

A

N

Q

I

T

V

I

A

A

Q

T

F

F

G

G

R

R

I

L

D

D

G

G

L

L

V

V

N

N

A

A

G

G

V

V

N

N

D

D

G

G

V

I

G

G

L

L

E

D

S

A

G

G

N

R

Y

-

K

D

D

A

F

F

M

V

A

A

S

S

L

L

H

E

K

R

N

N

V

L

V

I

H

H

Y

Y

L

A

M

M

A

A

H

H

H

Y

V

C

L

V

P

P

E

H

L

L

I

K

K

A

S

T

K

R

G

G

A

A

I

I

V

V

N

N

I

I

T

S

S
|
S

K

K

T

T

A

A

E

V

T

T

G

G

Q

Q

G

G

N

N

T
|
T

S

S

A

G

Y
|
Y

A

C

A

A

A

S

N
x
K

G

G

G

A

R

Q

N

L

A

A

L

L

T

T

R

R

E

E

W

W

A

A

V

V

E

A

L

L

L

R

K

E

Y

H

G

G

I

V

R

R

V

V

N

N

A

A

V

V

V

I

V

P

A

A

E

E

C

V

W

M

T

T

P

P

A

L

Y

Y

E

R

T

N

W
|
W

I

I

E

A

T

T

L

F

D

E

N

D

A

P

E

E

E

A

K

K

L

L

K

A

E

E

I

I

T

A

A

A

K

K

I

V

P

P

L

L

E

G

N

R

R

R

M

F

T

T

A

P

E

D

E

E

I

I

A

A

N

D

M

T

T

A

A

V

F

F

L

L

M

L

S

S

S
x
P

K

R

S

A

S
|
S

H

H

T

T

G

G

Q

E

I

W

I

L

H

F

V

V

D

D

G

G

Y

Y

V

T

H

H

L

L

D

D

R

R

A

A

L

L

active site: G18 (= G18), S140 (= S142), T150 (= T152), Y153 (= Y155), K157 (≠ N159), W194 (= W196)
binding calcium ion: P234 (≠ S236), S237 (= S239)

A0A0H3KNE7 L-fucose dehydrogenase; EC 1.1.1.435 from Burkholderia multivorans (strain ATCC 17616 / 249) (see paper)
53% identity, 99% coverage: 1:259/262 of query aligns to 1:257/258 of A0A0H3KNE7

query
sites
A0A0H3KNE7

M

M

D

D

L

L

Q

N

L

L

K

Q

E

D

K

K

V

V

I

V

V

I

I

V

T

T

G

G

A

G

A

A

K
x
S

G

G

I
|
I

G

G

R

G

S

A

I

I

A

S

E

M

V

R

F

L

A

A

K

E

E

E

N

R

A

A

I

I

A

P

V

V

I

V

V

F

G

A

R
|
R

K
x
H

A

A

E

P

D

D

N

G

Q

A

I

F

V

L

V

-

D

D

A

A

I

L

A

A

T

Q

N

R

G

Q

G

P

K

R

A

A

A

T

Q

Y

F

L

V

P

A

V

E
|
E

L
|
L

S

Q

N

D

P

D

E

A

D

Q

C

C

E

R

T

D

V

A

V

V

K

A

N

Q

I

T

V

I

A

A

Q

T

F

F

G

G

R

R

I

L

D

D

G

G

L

L

V

V

N

N

N
|
N

A

A

G

G

V

V

N
|
N

D

D

G

G

V

I

G

G

L

L

E

D

S

A

G

G

N

R

Y

-

K

D

D

A

F

F

M

V

A

A

S

S

L

L

H

E

K

R

N

N

V

L

V

I

H

H

Y

Y

L

A

M

M

A

A

H

H

H

Y

V

C

L

V

P

P

E

H

L

L

I

K

K

A

S

T

K

R

G

G

A

A

I

I

V

V

N

N

I

I

T

S

S
|
S

K
|
K

T

T

A

A

E

V

T

T

G

G

Q
|
Q

G

G

N

N

T

T

S

S

A

G

Y
|
Y

A

C

A

A

A

S

N
x
K

G

G

G

A

R

Q

N

L

A

A

L

L

T

T

R

R

E

E

W

W

A

A

V

V

E

A

L

L

L

R

K

E

Y

H

G

G

I

V

R

R

V

V

N

N

A

A

V

V

V

I

V

P

A
|
A

E
|
E

C
x
V

W

M

T
|
T

P

P

A

L

Y

Y

E

R

T

N

W

W

I

I

E

A

T

T

L

F

D

E

N

D

A

P

E

E

E

A

K

K

L

L

K

A

E

E

I

I

T

A

A

A

K

K

I

V

P

P

L

L

E

G

N

R

R

R

M

F

T

T

A

P

E

D

E

E

I

I

A

A

N

D

M

T

T

A

A

V

F

F

L

L

M

L

S

S

S

P

K

R

S

A

S

S

H

H

T

T

G

G

Q

E

I

W

I

L

H

F

V

V

D

D

G

G

Y

Y

V

T

H

H

L

L

D

D

R

R

A

A

L

L

S17 (≠ K17) binding
I19 (= I19) binding
R39 (= R39) binding
H40 (≠ K40) binding
E63 (= E64) binding
L64 (= L65) binding
N90 (= N91) binding
N94 (= N95) binding
S140 (= S142) binding
K141 (= K143) binding
Q147 (= Q149) binding
Y153 (= Y155) binding ; binding
K157 (≠ N159) binding
A184 (= A186) binding
E185 (= E187) binding
V186 (≠ C188) binding
T188 (= T190) binding

6ds1B Crystal structure of cj0485 dehydrogenase in complex with NADP+ (see paper)
45% identity, 99% coverage: 1:259/262 of query aligns to 1:257/260 of 6ds1B

query
sites
6ds1B

M

M

D

D

L

L

Q

K

L

I

K

K

E

N

K

K

V

V

I

C

V

I

I

I

T

T

G
|
G

A

G

A

A

K
|
K

G
|
G

I
|
I

G

G

R

Y

S

G

I

I

A

A

E

K

V

L

F

W

A

A

K

S

E

E

N

G

A

G

I

I

A

P

V

V

I

I

V

F

G
x
S

R
|
R

-

S

-

M

-

P

K

K

A

E

E

H

D

D

N

K

Q

E

I

L

V

-

D

-

A

-

I

-

A

K

T

K

N

L

G

S

K

E

A

Y

A

E

Q

F

F

Y

V

E

A
x
I

E
x
D

L
|
L

S

K

N

N

P

Y

E

E

D

Q

C

I

E

E

T

K

V

L

V

V

K

K

N

K

I

V

V

A

A

I

Q

K

F

H

G

G

R

G

I

I

D

Y

G

A

L

L

V

V

N

N

N
|
N

A
|
A

G

G

V

T

N

N

D

D

G

N

V

L

G

H

L

I

E

E

S

N

G

T

N

S

Y

T

K

Q

D

D

F

L

M

I

A

K

S

S

L

Y

H

E

K

N

N

N

V

L

V

F

H

H

Y

Y

L

T

M

M

A

T

H

K

H

E

V

C

L

L

P

P

E

Y

L

I

I

K

K

K

S

E

K

Q

G

G

A

S

I

I

V

L

N

N

I
|
I

T
x
V

S
|
S

K

K

T

T

A

G

E

I

T

T

G

G

Q

Q

G

G

N

R

T

T

S

S

A

A

Y
|
Y

A

A

A

S

A

A

N
x
K

G

A

R

Q

N

M

A

G

L

F

T

T

R

R

E

E

W

W

A

A

V

C

E

A

L

F

L

A

K

K

Y

D

G

N

I

V

R

R

V

V

N

N

A

A

V

I

V

A

V
x
P

A
|
A

E

E

C
x
V

W

M

T

T

P

P

A

L

Y

Y

E

E

T

K

W

W

I

L

E

Q

T

N

L

F

D

P

N

N

A

P

E

K

E

E

K

Q

L

Y

K

E

E

K

I

I

T

A

A

K

K

A

I

I

P

P

L

L

E

G

N

H

R

R

M

F

T

T

A

I

E

E

I

I

A

A

N

N

M

T

T

A

A

V

F

F

L

T

M

L

S

S

S

P

K

L

S

A

S

S

H

H

T

T

G

G

Q

Q

I

I

I

L

H

M

V

P

D

D

G

G

Y

Y

V

V

H

H

L

L

D

D

R

R

A

A

L

L

binding magnesium ion: I61 (≠ A63), D62 (≠ E64)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G14 (= G14), K17 (= K17), G18 (= G18), I19 (= I19), S38 (≠ G38), R39 (= R39), D62 (≠ E64), L63 (= L65), N89 (= N91), A90 (= A92), I138 (= I140), V139 (≠ T141), S140 (= S142), Y153 (= Y155), K157 (≠ N159), P183 (≠ V185), A184 (= A186), V186 (≠ C188)

4nbuB Crystal structure of fabg from bacillus sp (see paper)
34% identity, 95% coverage: 4:251/262 of query aligns to 4:241/244 of 4nbuB

query
sites
4nbuB

Q

R

L

L

K

Q

E

D

K

K

V

V

I

A

V

I

I

I

T

T

G
|
G

A

A

K
x
N

G
|
G

I
|
I

G

G

R

L

S

E

I

A

A

A

E

R

V

V

F

F

A

M

K

K

E

E

N

G

A

A

I

K

A

V

V

V

I

I

V

-

G

-

R

-

K

-

A

A

E
x
D

D
x
F

N

N

Q

E

I

A

V

A

-

G

V

K

D

E

A

A

I

V

A

E

T

A

N

N

G

P

G

G

K

-

A

-

A

V

Q

V

F

F

V

I

-

R

A
x
V

E
x
D

L
x
V

S

S

N

D

P

R

E

E

D

S

C

V

E

H

T

R

V

L

V

V

K

E

N

N

I

V

V

A

A

E

Q

R

F

F

G

G

R

K

I

I

D

D

G

I

L

L

V

I

N

N

N
|
N

A
|
A

G
|
G

V
x
I

N

T

D

R

G
x
D

V

S

G

M

L

L

E

S

S
x
K

G

M

N

T

Y

V

K

D

D

Q

F

F

M

Q

A

Q

S

V

L

I

H

N

K

V

N
|
N

V

L

V

T

H

G

Y

V

Y

F

L

H

M

C

A

T

H

Q

H

A

V

V

L

L

P

P

E

Y

L

M

I

A

-

E

K

Q

S

G

K

K

G

G

A

K

I

I

V

I

N

N

I
x
T

T

S

S
|
S

K

V

T

T

A

G

E

T

T

Y

G

G

Q
x
N

G
x
V

N

G

T
x
Q

S

T

A

N

Y
|
Y

A

A

N
x
K

G

A

G

G

R

V

N

I

A

G

L

M

T

T

R

K

E

T

W

W

A

A

V

K

E

E

L

L

L

A

K

R

Y

K

G

G

I

I

R

N

V

V

N

N

A

A

V

V

V

-

A

-

E

-

C

-

W

-

T

A

P
|
P

A

G

Y
x
F

E
x
T

T

E

W
x
T

I

A

E
x
M

T

V

L

A

D

E

N

V

A

P

E

E

E

K

K

V

L

I

K

E

E
x
K

I

M

T

K

A

A

K

Q

I

V

P

P

L

M

E

-

N

G

R

R

M

L

T

G

T

K

A

P

E

E

E

D

I

I

A

A

N

N

M

A

T

Y

A

L

F

F

L

L

M

A

S

S

S

H

K

E

S

S

S

D

H

Y

T

V

T

N

G

G

Q

H

I

V

I

L

H

H

V

V

D

D

G

G

active site: G18 (= G18), N111 (= N115), S139 (= S142), Q149 (≠ T152), Y152 (= Y155), K156 (≠ N159)
binding acetoacetyl-coenzyme a: D93 (≠ G97), K98 (≠ S102), S139 (= S142), N146 (≠ Q149), V147 (≠ G150), Q149 (≠ T152), Y152 (= Y155), F184 (≠ Y193), M189 (≠ E198), K200 (≠ E209)
binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G14), N17 (≠ K17), G18 (= G18), I19 (= I19), D38 (≠ E42), F39 (≠ D43), V59 (≠ A63), D60 (≠ E64), V61 (≠ L65), N87 (= N91), A88 (= A92), G89 (= G93), I90 (≠ V94), T137 (≠ I140), S139 (= S142), Y152 (= Y155), K156 (≠ N159), P182 (= P191), F184 (≠ Y193), T185 (≠ E194), T187 (≠ W196), M189 (≠ E198)

1vl8B Crystal structure of gluconate 5-dehydrogenase (tm0441) from thermotoga maritima at 2.07 a resolution
32% identity, 95% coverage: 3:252/262 of query aligns to 2:249/252 of 1vl8B

query
sites
1vl8B

L

F

Q

D

L

L

K

R

E

G

K

R

V

V

I

A

V

L

I

V

T

T

G
|
G

A

G

A

S

K
x
R

G
|
G

I
x
L

G

G

R

F

S

G

I

I

A

A

E

Q

V

G

F

L

A

A

K

E

E

A

N

G

A

C

I

S

A

V

V

V

I

V

V

A

G
x
S

R
|
R

K

N

A

L

E

E

D

E

N

A

Q

S

I

E

V

A

D

Q

A

K

I

L

A

T

T

E

N

K

G

Y

G

G

-

V

K

E

A

T

A

M

Q

A

F

F

V

R

A
x
C

E
x
D

L
x
V

S

S

N

N

P

Y

E

E

D

E

C

V

E

K

T

K

V

L

K

E

N

A

I

V

V

K

A

E

Q

K

F

F

G

G

R

K

I

L

D

D

G

T

L

V

V

V

N

N

N
x
A

A
|
A

G
|
G

V
x
I

N

N

D

R

G

R

V

H

G

P

L

A

E

E

S

E

G

F

N

P

Y

L

K

D

D

E

F

F

M

R

A

Q

S

V

L

I

H

E

K
x
V

N

N

V

L

V

F

H

G

Y

T

Y

Y

L

Y

M

V

A

C

H

R

H

E

V

A

L

F

P

S

E

L

L

L

I

R

K

E

S

S

K

D

G

N

-

P

A

S

I

I

V

I

N

N

I
|
I

T

G

S
|
S

K

L

T

T

A

V

E

E

-

E

T

V

G

T

Q

M

G

P

N

N

T
x
I

S

S

A

A

Y
|
Y

A

A

A

S

N
x
K

G

G

R

V

N

A

A

S

L

L

T

T

R

K

E

A

W

L

A

A

V

K

E

E

L

W

L

G

K

R

Y

Y

G

G

I

I

R

R

V

V

N

N

A

-

V

-

V

V

V

I

A

A

E
x
P

C
x
G

W

W

T

-

P

-

A

-

Y
|
Y

E

R

T
|
T

W

K

I
x
M

-
x
T

E

E

T

A

L

V

D

F

N

S

A

D

E

P

E

E

K

K

L

L

K

D

E

Y

I

M

T

L

A

K

K

R

I

I

P

P

L

L

E

-

N

G

R

R

M

T

T

G

T

V

A

P

E

E

E

D

I

L

A

K

N

G

M

V

T

A

A

V

F

F

L

L

M

A

S

S

S

E

K

E

S

A

S

K

H

Y

T

V

T

T

G

G

Q

Q

I

I

H

F

V

V

D

D

G

G

Y

W

active site: G17 (= G18), S143 (= S142), I154 (≠ T152), Y157 (= Y155), K161 (≠ N159)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G13 (= G14), R16 (≠ K17), G17 (= G18), L18 (≠ I19), S37 (≠ G38), R38 (= R39), C63 (≠ A63), D64 (≠ E64), V65 (≠ L65), A91 (≠ N91), A92 (= A92), G93 (= G93), I94 (≠ V94), V114 (≠ K114), I141 (= I140), S143 (= S142), Y157 (= Y155), K161 (≠ N159), P187 (≠ E187), G188 (≠ C188), Y190 (= Y193), T192 (= T195), M194 (≠ I197), T195 (vs. gap)

1iy8A Crystal structure of levodione reductase (see paper)
33% identity, 95% coverage: 4:251/262 of query aligns to 1:253/258 of 1iy8A

query
sites
1iy8A

Q

R

L

F

K

T

E

D

K

R

V

V

I

V

V

L

I

I

T

T

G
|
G

A

G

K
x
S

G
|
G

I
x
L

G

G

R

R

S

A

I

T

A

A

E

V

V

R

F

L

A

A

K

A

E

E

N

G

A

A

I

K

A

L

V

S

I

L

V

V

G
x
D

R
x
V

K

S

A

S

E

E

D

G

N

L

Q

E

I

A

V

S

V

K

D

A

A

A

I

V

A

L

T

E

N

T

G

A

G

P

K

D

A

A

A

E

Q

V

F

L

-

T

V

V

A
|
A

E
x
D

L
x
V

S

S

N

D

P

E

E

A

D

Q

C

V

E

E

T

A

V

Y

V

V

K

T

N

A

I

T

V

T

A

E

Q

R

F

F

G

G

R

R

I

I

D

D

G

G

L

F

V

F

N

N

N
|
N

A

A

G
|
G

V
x
I

N

E

D

G

G

K

V

Q

G

N

-

P

L

T

E

E

S

S

G

F

N

T

Y

A

K

A

D

E

F

F

M

D

A

K

S

V

L

V

H

S

K

I

N

N

V

L

V

R

H

G

Y

V

Y

F

L

L

M

G

A

L

H

E

H

K

V

V

L

L

P

-

E

K

L

I

I

M

K

R

S

E

K

Q

G

G

A

S

-

G

-

M

I

V

V

V

N

N

I
x
T

T

A

S
|
S

K

V

T

G

A

G

E

I

T

R

G

G

Q

I

G

G

N

N

T
x
Q

S

S

A

G

Y
|
Y

A

A

N
x
K

G

H

G

G

R

V

N

V

A

G

L

L

T

T

R

R

E

N

W

S

A

A

V

V

E

E

L

Y

L

G

K

R

Y

Y

G

G

I

I

R

R

V

I

N

N

A

A

V

I

V

A

V
x
P

A
x
G

E

A

C

I

W

W

T
|
T

P
|
P

A
x
M

Y

V

E

E

T

N

W

S

I

M

E

K

T

Q

L

L

D

D

-

P

-

E

N

N

A

P

E

R

E

K

K

A

L

A

K

E

E

E

I

F

T

I

A

Q

K

V

I

N

P

P

L

-

E

S

N

K

R

R

M

Y

T

G

T

E

A

A

E

P

E

E

I

I

A

A

N

A

M

V

T

V

A

A

F

F

L

L

M

L

S

S

S

D

K

D

S

A

S

S

H

Y

T

V

T

N

G

A

Q

T

I

V

H

P

V

I

D

D

G

G

active site: G15 (= G18), S143 (= S142), Q153 (≠ T152), Y156 (= Y155), K160 (≠ N159)
binding nicotinamide-adenine-dinucleotide: G11 (= G14), S14 (≠ K17), G15 (= G18), L16 (≠ I19), D35 (≠ G38), V36 (≠ R39), A62 (= A63), D63 (≠ E64), V64 (≠ L65), N90 (= N91), G92 (= G93), I93 (≠ V94), T141 (≠ I140), S143 (= S142), Y156 (= Y155), K160 (≠ N159), P186 (≠ V185), G187 (≠ A186), T191 (= T190), P192 (= P191), M193 (≠ A192)

Q9LBG2 Levodione reductase; (6R)-2,2,6-trimethyl-1,4-cyclohexanedione reductase; EC 1.1.1.- from Leifsonia aquatica (Corynebacterium aquaticum) (see paper)
33% identity, 95% coverage: 4:251/262 of query aligns to 10:262/267 of Q9LBG2

query
sites
Q9LBG2

Q

R

L

F

K

T

E

D

K

R

V

V

I

V

V
x
L

I
|
I

T
|
T

G
|
G

A
x
G

K
x
S

G
|
G

I
x
L

G
|
G

R
|
R

S
x
A

I
x
T

A
|
A

E
x
V

V
x
R

F
x
L

A
|
A

K
x
A

E
|
E

N
x
G

A
|
A

I
x
K

A
x
L

V
x
S

I
x
L

V

V

G

D

R

V

K

S

A

S

E

E

D

G

N

L

Q

E

I

A

V

S

V

K

D

A

A

A

I

V

A

L

T

E

N

T

G

A

G

P

K

D

A

A

A

E

Q

V

F

L

-

T

V

V

A

A

E

D

L

V

S

S

N

D

P

E

E

A

D

Q

C

V

E

E

T

A

V

Y

V

V

K

T

N

A

I

T

V

T

A

E

Q

R

F

F

G

G

R

R

I

I

D

D

G

G

L

F

V

F

N

N

A

A

G

G

V

I

N
x
E

D

G

G

K

V

Q

G

N

-

P

L

T

E

E

S

S

G

F

N

T

Y

A

K

A

D

E

F

F

M

D

A

K

S

V

L

V

H

S

K

I

N

N

V

L

V

R

H

G

Y

V

Y

F

L

L

M

G

A

L

H

E

H

K

V

V

L

L

P

-

E

K

L

I

I

M

K

R

S

E

K

Q

G

G

A

S

-

G

-

M

I

V

V

V

N

N

I

T

T

A

S

S

K

V

T

G

A

G

E

I

T

R

G

G

Q

I

G

G

N

N

T

Q

S

S

A

G

Y

Y

A

A

N

K

G

H

G

G

R

V

N

V

A

G

L

L

T

T

R

R

E

N

W

S

A

A

V

V

E

E

L

Y

L

G

K

R

Y

Y

G

G

I

I

R

R

V

I

N

N

A

A

V

I

V

A

V

P

A

G

E

A

C

I

W

W

T

T

P

P

A

M

Y

V

E

E

T

N

W

S

I

M

E

K

T

Q

L

L

D

D

-

P

-

E

N

N

A

P

E

R

E

K

K

A

L

A

K

E

E

E

I

F

T

I

A

Q

K

V

I

N

P

P

L

-

E

S

N

K

R

R

M

Y

T

G

T

E

A

A

E

P

E

E

I

I

A

A

N

A

M

V

T

V

A

A

F

F

L

L

M

L

S

S

S

D

K

D

S

A

S

S

H

Y

T

V

T

N

G

A

Q

T

I

V

H

P

V

I

D

D

G

G

17:42 (vs. 11:36, 38% identical) binding
E103 (≠ N95) mutation E->A,D,N,Q: 26-fold increase in Km and a much lower enantiomeric excess of the reaction products.

4i08A Crystal structure of beta-ketoacyl-acyl carrier protein reductase (fabg) from vibrio cholerae in complex with NADPH (see paper)
32% identity, 95% coverage: 3:251/262 of query aligns to 4:239/243 of 4i08A

query
sites
4i08A

L

M

Q

N

L

L

K

E

E

G

K

K

V

V

I

A

V

L

I

V

T

T

G
|
G

A

A

A
x
S

K
x
R

G
|
G

I
|
I

G

G

R

K

S

A

I

I

A

A

E

E

V

L

F

L

A

A

K

E

E

R

N

G

A

A

I

K

A

V

V

I

I

G

V

T

G

A

R
x
T

K

S

A

E

E

S

D

G

N

A

Q

Q

I

A

V

I

V

S

D

D

A

Y

I

L

A

G

T

D

N

N

G

G

G

K

K

G

A

M

A

A

Q

L

F
x
N

V
|
V

A

-

E

-

L

-

S

T

N

N

P

P

E

E

D

S

C

I

E

E

T

A

V

V

V

L

K

K

N

A

I

I

V

T

A

D

Q

E

F

F

G

G

R

G

I

V

D

D

G

I

L

L

V

V

N

N

N
|
N

A
|
A

G

G

V

I

N

T

D

R

G

D

V

N

G

L

L

L

E

M

S

R

G

M

N

K

Y

E

K

E

D

E

F

W

M

S

A

D

S

I

L

M

H

E

K

T

N
|
N

V

L

V

T

H

S

Y

I

Y

F

L

R

M

L

A

S

H

K

H

A

V

V

L

L

P

R

E

G

L

M

I

M

K

K

S

K

K

R

-

Q

G

G

A

R

I

I

V

I

N

N

I

V

T
x
G

S
|
S

K

V

T

V

A

G

E

T

T

M

G

G

Q

N

G

A

N

G

T
x
Q

S

A

A

N

Y
|
Y

A

A

N
x
K

G

A

G

G

R

V

N

I

A

G

L

F

T

T

R

K

E

S

W

M

A

A

V

R

E

E

L

V

L

A

K

S

Y

R

G

G

I

V

R

T

V

V

N

N

A

T

V

V

V

A

V

-

A

-

E

-

C

-

W

-

T

-

P
|
P

A
x
G

Y

F

E

-

T

-

W

-

I

I

E

E

T
|
T

L

D

D

M

N

N

A

D

E

E

E

Q

K

R

L

T

K

A

E

T

I

L

T

-

A

A

K

Q

I

V

P

P

L

-

E

A

N

G

R

R

M

L

T

G

T

D

A

P

E

R

E

E

I

I

A

A

N

S

M

A

T

V

A

A

F

F

L

L

M

A

S

S

S

P

K

E

S

A

H

Y

T

I

T

T

G

G

Q

E

I

T

I

L

H

H

V

V

D

N

G

G

active site: G19 (= G18), N113 (= N115), S141 (= S142), Q151 (≠ T152), Y154 (= Y155), K158 (≠ N159)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G15 (= G14), S17 (≠ A16), R18 (≠ K17), I20 (= I19), T40 (≠ R39), N62 (≠ F61), V63 (= V62), N89 (= N91), A90 (= A92), G140 (≠ T141), S141 (= S142), Y154 (= Y155), K158 (≠ N159), P184 (= P191), G185 (≠ A192), T189 (= T199)

Q9KJF1 (2S)-[(R)-hydroxy(phenyl)methyl]succinyl-CoA dehydrogenase subunit BbsD; (S,R)-2-(alpha-hydroxybenzyl)succinyl-CoA dehydrogenase subunit BbsD; EC 1.1.1.429 from Thauera aromatica (see 2 papers)
32% identity, 95% coverage: 3:251/262 of query aligns to 1:242/248 of Q9KJF1

query
sites
Q9KJF1

L
x
M

Q

G

L

I

K

Q

E

N

K

R

V

V

I

A

V

L

I

I

T

T

G

G

A

S

A

A

K
x
S

G

G

I

M

G

G

R

K

S

Q

I

T

A

A

E

L

V

R

F

F

A

A

K

E

E

Q

N

G

A

A

I

A

A

V

V

V

I

I

V

N

G
x
D

R

I

K

D

A

A

E

E

D

K

N

V

Q

R

I

A

V

T

V

V

D

D

A

E

I

F

A

S

T

A

N

R

G

G

G

H

K

R

A

V

A

L

Q

G

F

A

V

V

A

A

E
x
D

L
x
I

S

G

N

N

P

K

E

A

D

A

C

V

E

D

T

G

V

M

V

V

K

K

N

Q

I

T

V

I

A

D

Q

A

F

F

G

G

R

R

I

I

D

D

G

I

L

L

V

V

N

N

N
|
N

A

A

G

G

V

M

N

E

D

R

G

A

V

G

G

A

L

L

E

R

S

K

G

L

N

S

Y

E

K

A

D

D

F

W

M

D

A

V

S

T

L

I

H

N

K

V

N

N

V

L

V

K

H

G

Y

T

Y

F

L

L

M

C

A

T

H

Q

H

A

V

V

L

H

P

G

E

H

L

M

I

V

K

E

S

N

K

K

-

H

G

G

A

R

I

I

V

V

N

N

I

I

T

A

S

S

K

R

T

A

A

W

E

L

T

G

G

G

Q

A

G

G

N

Q

T

T

S

P

A

-

Y
|
Y

A

S

A

A

N
x
K

G

A

G

G

R

V

N

V

A

G

L

M

T

T

R

R

E

A

W

L

A

A

V

I

E

E

L

L

L

G

K

R

Y

A

G

G

I

I

R

T

V

V

N

N

A

C

V

V

V

A

V

P

A

G

E

L

C

I

W

H

T

T

P

P

A

M

Y

W

E

-

T

-

W

-

I

-

E

-

T

-

L

-

D

D

N

E

A

L

E

P

E

E

K

K

L

D

K

Q

E

Q

I

F

T

L

A

L

K

S

I

R

P

Q

L

P

E

T

N

G

R

K

M

L

T

G

T

E

A

P

E

D

I

I

A

A

N

N

M

T

T

L

A

L

F

F

L

L

M

A

S

D

K

D

S

S

S

G

H

F

T

V

T

T

G

G

Q

Q

I

V

I

L

H

Y

V

V

D

C

G

G

M1 (≠ L3) modified: Initiator methionine, Removed
S15 (≠ K17) binding
D36 (≠ G38) binding
D62 (≠ E64) binding
I63 (≠ L65) binding
N89 (= N91) binding
Y153 (= Y155) binding
K157 (≠ N159) binding

4iiuA Crystal structure of a putative 3-oxoacyl-[acyl-carrier protein]reductase from escherichia coli strain cft073 complexed with NADP+ at 2.1 a resolution
31% identity, 94% coverage: 7:253/262 of query aligns to 2:243/243 of 4iiuA

query
sites
4iiuA

E

S

K

R

V

S

I

V

V

L

I

V

T

T

G
|
G

A

A

A
x
S

K
|
K

G
|
G

I
|
I

G

G

R

R

S

A

I

I

A

A

E

R

V

Q

F

L

A

A

K

A

E

D

N

G

A

F

-

N

I

I

A

G

V

V

I
x
H

V

Y

G
x
H

R

R

K
x
D

A

A

E

A

D

G

N

A

Q

Q

I

E

V

T

V

L

D

N

A

A

I

I

A

V

T

A

N

N

G

G

K

N

A

G

A

R

Q

L

F

L

V

S

A

F

E
x
D

L
x
V

S

A

N

N

P

R

E

E

D

Q

C

C

E

R

T

E

V

V

V

L

K

E

N

H

I

E

V

I

A

A

Q

Q

F

H

G

G

R

A

I

W

D

Y

G

G

L

V

V

V

N

S

N
|
N

A
|
A

G
|
G

V
x
I

N

A

D

R

G

D

V

A

G

A

L

F

E

P

S

A

G

L

N

S

Y

N

K

D

D

D

F

W

M

D

A

A

S

V

L

I

H

H

K

T

N

N

V

L

V

D

H

S

Y

F

Y

Y

L

N

M

V

A

I

H

Q

H

P

V

C

L

I

P

M

E

P

L

M

I

I

K

G

S

A

K

R

-

Q

-

G

G

G

A

R

I

I

V

I

N

T

I

L

T

S

S
|
S

K

V

T

S

A

G

E

V

T

M

G

G

Q

N

G

R

N

G

T

Q

S

V

A

N

Y
|
Y

A

S

A

A

N
x
K

G

A

G

G

R

I

N

I

A

G

L

A

T

T

R

K

E

A

W

L

A

A

V

I

E

E

L

L

L

A

K

K

Y

R

G

K

I

I

R

T

V

V

N

N

A

-

V

-

A

-

E

-

C

C

W

I

T

A

P
|
P

A
x
G

Y

L

E

-

T

-

W

-

I
|
I

E

D

T
|
T

-

G

-
x
M

L
x
I

D

E

N

M

A

E

E

E

E

S

K

A

L

L

K

K

E

E

I

A

T

M

A

S

K

M

I

I

P

P

L

M

E

K

N

-

R

R

M

M

T

G

T

Q

A

A

E

E

I

V

A

A

N

G

M

L

T

A

A

S

F

Y

L

L

M

M

S

S

S

D

K

I

S

A

S

G

H

Y

T

V

T

T

G

R

Q

Q

I

V

I

I

H

S

V

I

D

N

G

G

Y

M

V

L

active site: G13 (= G18), S140 (= S142), Y153 (= Y155), K157 (≠ N159)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G9 (= G14), S11 (≠ A16), K12 (= K17), G13 (= G18), I14 (= I19), H32 (≠ I36), H34 (≠ G38), D36 (≠ K40), D60 (≠ E64), V61 (≠ L65), N87 (= N91), A88 (= A92), G89 (= G93), I90 (≠ V94), S140 (= S142), Y153 (= Y155), K157 (≠ N159), P183 (= P191), G184 (≠ A192), I186 (= I197), T188 (= T199), M190 (vs. gap), I191 (≠ L200)

7pcsB Structure of the heterotetrameric sdr family member bbscd (see paper)
32% identity, 94% coverage: 5:251/262 of query aligns to 2:241/247 of 7pcsB

query
sites
7pcsB

L

I

K

Q

E

N

K

R

V

V

I

A

V

L

I

I

T

T

G
|
G

A

S

A

A

K

S

G

G

I
x
M

G

G

R

K

S

Q

I

T

A

A

E

L

V

R

F

F

A

A

K

E

E

Q

N

G

A

A

I

A

A

V

V

V

I

I

V

N

G
x
D

R
x
I

K

D

A

A

E

E

D

K

N

V

Q

R

I

A

V

T

V

V

D

D

A

E

I

F

A

S

T

A

N

R

G

G

G

H

K

R

A

V

A

L

Q

G

F

A

V

V

A

A

E

D

L
x
I

S

G

N

N

P

K

E

A

D

A

C

V

E

D

T

G

V

M

V

V

K

K

N

Q

I

T

V

I

A

D

Q

A

F

F

G

G

R

R

I

I

D

D

G

I

L

L

V

V

N

N

N
|
N

A

A

G
|
G

V

M

N

E

D

R

G

A

V

G

G

A

L

L

E

R

S

K

G

L

N

S

Y

E

K

A

D

D

F

W

M

D

A

V

S

T

L

I

H

N

K

V

N

N

V

L

V

K

H

G

Y

T

Y

F

L

L

M

C

A

T

H

Q

H

A

V

V

L

H

P

G

E

H

L

M

I

V

K

E

S

N

K

K

-

H

G

G

A

R

I

I

V

V

N

N

I
|
I

T

A

S
|
S

K

R

T

A

A

W

E

L

T

G

G

G

Q

A

G

G

N

Q

T

T

S

P

A

-

Y
|
Y

A

S

A

A

N
x
K

G

A

G

G

R

V

N

V

A

G

L

M

T

T

R

R

E

A

W

L

A

A

V

I

E

E

L

L

L

G

K

R

Y

A

G

G

I

I

R

T

V

V

N

N

A

C

V

V

V

A

V

P

A

G

E

L

C
x
I

W

H

T

T

P

P

A

M

Y

W

E

-

T

-

W

-

I

-

E

-

T

-

L

-

D

D

N

E

A

L

E

P

E

E

K

K

L

D

K

Q

E

Q

I

F

T

L

A

L

K

S

I

R

P

Q

L

P

E

T

N

G

R

K

M

L

T

G

T

E

A

P

E

D

I

I

A

A

N

N

M

T

T

L

A

L

F

F

L

L

M

A

S

D

K

D

S

S

S

G

H

F

T

V

T

T

G

G

Q

Q

I

V

I

L

H

Y

V

V

D

C

G

G

binding nicotinamide-adenine-dinucleotide: G11 (= G14), M16 (≠ I19), D35 (≠ G38), I36 (≠ R39), I62 (≠ L65), N88 (= N91), G90 (= G93), I138 (= I140), S140 (= S142), Y152 (= Y155), K156 (≠ N159), I185 (≠ C188)

3ijrF 2.05 angstrom resolution crystal structure of a short chain dehydrogenase from bacillus anthracis str. 'Ames ancestor' in complex with NAD+
32% identity, 95% coverage: 4:253/262 of query aligns to 43:287/290 of 3ijrF

query
sites
3ijrF

Q

K

L

L

K

K

E

G

K

K

V

N

I

V

V

L

I

I

T

T

G

G

A

G

A
x
D

K
x
S

G
|
G

I
|
I

G

G

R

R

S

A

I

V

A

S

E

I

V

A

F

F

A

A

K

K

E

E

N

G

A

A

-

N

I

I

A

A

V

I

I

A

V
x
Y

G
x
L

R

D

K
x
E

A

E

E

G

D

D

N

A

Q

N

I

E

V

T

V

K

D

Q

A

Y

I

V

A

E

T

K

N

E

G

G

G

V

K

K

A

C

A

V

Q

L

F

L

V

P

A
x
G

E
x
D

L
|
L

S

S

N

D

P

E

E

Q

D

H

C

C

E

K

T

D

V

I

V

V

K

Q

N

E

I

T

V

V

A

R

Q

Q

F

L

G

G

R

S

I

L

D

N

G

I

L

L

V

V

N

N

N
|
N

-
x
V

A
|
A

G
x
Q

V

Q

N

Y

D

P

G

Q

V

Q

G

G

L

L

E

E

S

Y

G

I

N

T

Y

A

K

E

D

Q

F

L

M

E

A

K

S

T

L

F

H

R

K
x
I

N

N

V

I

V

F

H

S

Y

Y

Y

F

L

H

M

V

A

T

H

K

H

A

V

A

L

L

P

S

E

H

L

L

I

-

K

K

S

Q

K

G

G

D

A

V

I

I

V

I

N

N

I
x
T

T

A

S
|
S

K

I

T

V

A

A

E

Y

T

E

G

G

Q

N

G

E

N

T

T
x
L

S

I

A

D

Y
|
Y

A

S

A

A

A

T

N
x
K

G

G

G

A

R

I

N

V

A

A

L

F

T

T

R

R

E

S

W

L

A

S

V

Q

E

S

L

L

L

V

K

Q

Y

K

G

G

I

I

R

R

V

V

N

N

A

G

V

V

V

A

V
x
P

A
x
G

E
x
P

C
x
I

W

W

T
|
T

P

P

A
x
L

Y

I

E

P

T

S

W

S

I

F

E

D

T

-

L

-

D

-

N

-

A

-

E

E

E
x
K

K

K

L

V

K

S

E

Q

I

F

T

G

A

S

K

N

I

V

P

P

L

M

E

Q

N

-

R

R

M

P

T

G

T

Q

A

P

E

Y

E

E

I

L

A

A

N

P

M

A

T

Y

A

V

F

Y

L

L

M

A

S

S

K

D

S

S

H

Y

T

V

T

T

G

G

Q

Q

I

M

I

I

H

H

V

V

D

N

G

G

Y

V

V

I

active site: G57 (= G18), S182 (= S142), L192 (≠ T152), Y195 (= Y155), K199 (≠ N159), K240 (≠ E205)
binding magnesium ion: D55 (≠ A16), S56 (≠ K17), E80 (≠ K40)
binding nicotinamide-adenine-dinucleotide: D55 (≠ A16), S56 (≠ K17), G57 (= G18), I58 (= I19), Y77 (≠ V37), L78 (≠ G38), E80 (≠ K40), G103 (≠ A63), D104 (≠ E64), L105 (= L65), N131 (= N91), V132 (vs. gap), A133 (= A92), Q134 (≠ G93), I155 (≠ K114), T180 (≠ I140), S182 (= S142), Y195 (= Y155), K199 (≠ N159), P225 (≠ V185), G226 (≠ A186), P227 (≠ E187), I228 (≠ C188), T230 (= T190), L232 (≠ A192)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 21

3i3oA 2.06 angstrom resolution crystal structure of a short chain dehydrogenase from bacillus anthracis str. 'Ames ancestor' in complex with NAD-acetone
32% identity, 95% coverage: 4:253/262 of query aligns to 35:279/282 of 3i3oA

query
sites
3i3oA

Q

K

L

L

K

K

E

G

K

K

V

N

I

V

V

L

I

I

T

T

G
|
G

A

G

A
x
D

K
x
S

G
|
G

I
|
I

G

G

R

R

S

A

I

V

A

S

E

I

V

A

F

F

A

A

K

K

E

E

N

G

A

A

-

N

I

I

A

A

V

I

I

A

V
x
Y

G
x
L

R

D

K
x
E

A

E

E

G

D

D

N

A

Q

N

I

E

V

T

V

K

D

Q

A

Y

I

V

A

E

T

K

N

E

G

G

G

V

K

K

A

C

A

V

Q

L

F

L

V

P

A
x
G

E
x
D

L
|
L

S

S

N

D

P

E

E

Q

D

H

C

C

E

K

T

D

V

I

V

V

K

Q

N

E

I

T

V

V

A

R

Q

Q

F

L

G

G

R

S

I

L

D

N

G

I

L

L

V

V

N

N

N
|
N

-
x
V

A
|
A

G
x
Q

V
x
Q

N

Y

D

P

G

Q

V

Q

G

G

L

L

E

E

S

Y

G

I

N

T

Y

A

K

E

D

Q

F

L

M

E

A

K

S

T

L

F

H

R

K
x
I

N

N

V

I

V

F

H

S

Y

Y

Y

F

L

H

M

V

A

T

H

K

H

A

V

A

L

L

P

S

E

H

L

L

I

-

K

K

S

Q

K

G

G

D

A

V

I

I

V

I

N

N

I
x
T

T

A

S
|
S

K

I

T

V

A

A

E

Y

T

E

G

G

Q

N

G

E

N

T

T
x
L

S

I

A

D

Y
|
Y

A

S

A

A

A

T

N
x
K

G

G

G

A

R

I

N

V

A

A

L

F

T

T

R

R

E

S

W

L

A

S

V

Q

E

S

L

L

L

V

K

Q

Y

K

G

G

I

I

R

R

V

V

N

N

A

G

V

V

V

A

V
x
P

A
x
G

E

P

C
x
I

W

W

T
|
T

P

P

A
x
L

Y

I

E

P

T

S

W

S

I

F

E

D

T

-

L

-

D

-

N

-

A

-

E

E

E
x
K

K

K

L

V

K

S

E

Q

I

F

T

G

A

S

K

N

I

V

P

P

L

M

E

Q

N

-

R

R

M

P

T

G

T

Q

A

P

E

Y

E

E

I

L

A

A

N

P

M

A

T

Y

A

V

F

Y

L

L

M

A

S

S

K

D

S

S

H

Y

T

V

T

T

G

G

Q

Q

I

M

I

I

H

H

V

V

D

N

G

G

Y

V

V

I

active site: G49 (= G18), S174 (= S142), L184 (≠ T152), Y187 (= Y155), K191 (≠ N159), K232 (≠ E205)
binding magnesium ion: D47 (≠ A16), S48 (≠ K17), E72 (≠ K40)
binding nicotinamide adenine dinucleotide acetone adduct: G45 (= G14), D47 (≠ A16), S48 (≠ K17), G49 (= G18), I50 (= I19), Y69 (≠ V37), L70 (≠ G38), E72 (≠ K40), G95 (≠ A63), D96 (≠ E64), L97 (= L65), N123 (= N91), V124 (vs. gap), A125 (= A92), Q126 (≠ G93), Q127 (≠ V94), I147 (≠ K114), T172 (≠ I140), S174 (= S142), Y187 (= Y155), K191 (≠ N159), P217 (≠ V185), G218 (≠ A186), I220 (≠ C188), T222 (= T190), L224 (≠ A192)

5t2uA Short chain dehydrogenase/reductase family protein (see paper)
33% identity, 95% coverage: 3:251/262 of query aligns to 2:237/241 of 5t2uA

query
sites
5t2uA

L

M

Q

E

L

L

K

E

E

G

K

L

V

T

I

A

V

L

I

V

T

T

G
|
G

A

G

A

T

K

S

G
|
G

I

I

G

G

R

L

S

E

I

S

A

A

E

R

V

L

F

M

A

A

K

A

E

E

N

G

A

A

I

D

A

V

V

V

I

I

V

T

G

G

R
|
R

K
x
D

A

A

E

Q

D
x
R

N

G

Q

E

I

-

V

-

D

-

A

Q

I

A

A

A

T

A

N

D

G

I

G

G

K

H

A

G

A

A

Q

R

F

F

V

V

-

Q

A

A

E
x
D

L
|
L

S

G

N

D

P

L

E

D

D

S

C

-

E

-

T

-

V

-

V

V

K

A

N

D

I

L

V

A

A

A

Q

Q

F

A

G

P

R

D

I

V

D

D

G

I

L

L

V

V

N

N

N
|
N

A
|
A

G

G

V

I

N
x
Y

D

P

G

Q

V

A

G

S

L

T

E

F

S

D

G

Q

N

D

Y

V

K

A

D

G

F

F

M

Q

A

Q

S

L

L

F

H

D

K

T

N

N

V

V

V

R

H

G

-

T

Y

Y

Y

F

L

L

M

V

A

A

H

A

V

A

L

K

P

G

E

M

L

V

I

A

K

R

S

G

K

H

G

G

A

S

I

I

V

V

N

N

I
|
I

T

T

S
x
T

K

L

T

A

A

A

E

H

T

K

G

G

Q

F

G

P

N

G

T
|
T

S

S

A

V

Y
|
Y

A

G

A

A

A

T

N
x
K

G

A

R

L

N

E

A

S

L

L

T

T

R

R

E

T

W

W

A

A

V

A

E

E

L

F

L

G

K

A

Y

N

G

G

I

V

R

R

V

V

N

N

A

S

V

V

V

S

V
x
P

A

G

E
x
P

C
x
T

W

R

T
|
T

P

P

A

-

Y

-

E

-

T

-

W

-

I

-

E
x
T

T
|
T

L

L

D

E

N

Q

A

L

E

G

E

D

K

F

L

I

K

D

E

D

I

V

T

A

A

A

K

G

I

L

P

P

L

L

E

R

N

-

R

R

M

T

T

A

A

P

E

E

I

I

A

A

N

Q

M

A

T

V

A

L

F

F

L

L

M

A

S

S

S

P

K

R

S

A

S

S

H

F

T

V

T

T

G

G

Q

S

I

T

I

L

H

Y

V

V

D

D

G

G

active site: G17 (= G18), T135 (≠ S142), T145 (= T152), Y148 (= Y155), K152 (≠ N159)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G13 (= G14), G17 (= G18), R38 (= R39), D39 (≠ K40), R42 (≠ D43), D60 (≠ E64), L61 (= L65), N83 (= N91), A84 (= A92), Y87 (≠ N95), I133 (= I140), T135 (≠ S142), Y148 (= Y155), K152 (≠ N159), P178 (≠ V185), P180 (≠ E187), T181 (≠ C188), T183 (= T190), T185 (≠ E198), T186 (= T199)

5itvA Crystal structure of bacillus subtilis bacc dihydroanticapsin 7- dehydrogenase in complex with nadh (see paper)
31% identity, 96% coverage: 1:252/262 of query aligns to 1:252/255 of 5itvA

query
sites
5itvA

M

M

D

I

L

M

Q

N

L

L

K

T

E

D

K

K

V

T

I

V

V

L

I

I

T

T

G
|
G

A

G

A

A

K
x
S

G
|
G

I
|
I

G

G

R

Y

S

A

I

A

A

V

E

Q

V

A

F

F

A

L

K

G

E

Q

N

Q

A

A

I

N

A

V

V

V

I

V

V

A

G
x
D

R
x
I

K

D

A

E

E

A

D

Q

N

G

Q

E

I

A

V

M

V

V

D

R

A

K

I

-

A

-

T

E

N

N

G

N

G

D

K

R

A

L

A

H

Q

F

F

V

V

Q

A
x
T

E

D

L
x
I

S

T

N

D

P

E

E

A

D

A

C

C

E

Q

T

H

V

A

V

V

K

E

N

S

I

A

V

V

A

H

Q

T

F

F

G

G

R

G

I

L

D

D

G

V

L

L

V

I

N

N

N
|
N

A

A

G
|
G

V

I

N

E

D

I

G

V

V

A

G

P

L

I

E

H

S

E

G

M

N

E

Y

L

K

S

D

D

F

W

M

N

A

K

S

V

L

L

H

Q

K

V

N

N

V

L

V

T

H

G

Y

M

Y

F

L

L

M

M

A

S

H

K

H

H

V

A

L

L

P

K

E

H

L

M

I

L

K

A

S

A

-

G

K

K

G

G

A

N

I

I

V

I

N

N

I
x
T

T

C

S
|
S

K

V

T

G

A

G

E

L

T

V

G

A

Q

W

G

P

N

D

T

I

S

P

A

A

Y
|
Y

A

N

A

A

A

S

N
x
K

G

G

R

V

N

L

A

Q

L

L

T

T

R

K

E

S

W

M

A

A

V

V

E

D

L

Y

L

A

K

K

Y

H

G

Q

I

I

R

R

V

V

N

N

A

C

V

V

V

C

V
x
P

A
x
G

E
x
I

C
x
I

W

D

T

T

P

P

A

L

Y

N

E

E

-

K

T

S

W

F

I

L

E

E

T

N

L

N

D

E

N

G

A

T

E

L

E

E

K

E

L

I

K

K

E

K

I

E

T

K

A

A

K

K

I

V

P

N

L

P

E

L

N

L

R

R

M

L

T

G

T

K

A

P

E

E

I

I

A

A

N

N

M

V

T

M

A

L

F

F

L

L

M

A

S

S

S

D

K

L

S

S

H

Y

T

M

T

T

G

G

Q

S

I

A

I

I

H

T

V

A

D

D

G

G

Y

Y

active site: G18 (= G18), S141 (= S142), Y154 (= Y155), K158 (≠ N159)
binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G14), S17 (≠ K17), G18 (= G18), I19 (= I19), D38 (≠ G38), I39 (≠ R39), T61 (≠ A63), I63 (≠ L65), N89 (= N91), G91 (= G93), T139 (≠ I140), S141 (= S142), Y154 (= Y155), K158 (≠ N159), P184 (≠ V185), G185 (≠ A186), I186 (≠ E187), I187 (≠ C188)

3op4A Crystal structure of putative 3-ketoacyl-(acyl-carrier-protein) reductase from vibrio cholerae o1 biovar eltor str. N16961 in complex with NADP+ (see paper)
31% identity, 95% coverage: 3:251/262 of query aligns to 4:243/247 of 3op4A

query
sites
3op4A

L

M

Q

N

L

L

K

E

E

G

K

K

V

V

I

A

V

L

I

V

T

T

G
|
G

A

A

A
x
S

K
x
R

G

G

I
|
I

G

G

R

K

S

A

I

I

A

A

E

E

V

L

F

L

A

A

K

E

E

R

N

G

A

A

I

K

A

V

V

I

I

G

V

T

G

A

R
x
T

K

S

A

E

E

S

D

G

N

A

Q

Q

I

A

V

I

V

S

D

D

A

Y

I

L

A

G

T

D

N

N

G

G

G

K

K

G

A

M

A

A

Q

L

F
x
N

V
|
V

A

-

E

-

L

-

S

T

N

N

P

P

E

E

D

S

C

I

E

E

T

A

V

V

V

L

K

K

N

A

I

I

V

T

A

D

Q

E

F

F

G

G

R

G

I

V

D

D

G

I

L

L

V

V

N

N

N
|
N

A
|
A

G

G

V
x
I

N

T

D

R

G

D

V

N

G

L

L

L

E

M

S

R

G

M

N

K

Y

E

K

E

D

E

F

W

M

S

A

D

S

I

L

M

H

E

K

T

N

N

V

L

V

T

H

S

Y

I

Y

F

L

R

M

L

A

S

H

K

H

A

V

V

L

L

P

R

E

G

L

M

I

M

K

K

S

K

K

R

-

Q

G

G

A

R

I

I

V

I

N

N

I
x
V

T

G

S
|
S

K

V

T

V

A

G

E

T

T

M

G

G

Q

N

G

A

N

G

T

Q

S

A

A

N

Y
|
Y

A

A

N
x
K

G

A

G

G

R

V

N

I

A

G

L

F

T

T

R

K

E

S

W

M

A

A

V

R

E

E

L

V

L

A

K

S

Y

R

G

G

I

V

R

T

V

V

N

N

A

T

V

V

V

A

V

-

A

-

E

-

C

-

W

-

T

-

P
|
P

A
x
G

Y

F

-
x
I

E

E

T
|
T

W

D

I
x
M

E

T

T

K

L

A

D

L

N

N

A

D

E

E

E

Q

K

R

L

T

K

A

E

T

I

L

T

-

A

A

K

Q

I

V

P

P

L

-

E

A

N

G

R

R

M

L

T

G

T

D

A

P

E

R

E

E

I

I

A

A

N

S

M

A

T

V

A

A

F

F

L

L

M

A

S

S

S

P

K

E

S

A

H

Y

T

I

T

T

G

G

Q

E

I

T

I

L

H

H

V

V

D

N

G

G

binding nadp nicotinamide-adenine-dinucleotide phosphate: G15 (= G14), S17 (≠ A16), R18 (≠ K17), I20 (= I19), T40 (≠ R39), N62 (≠ F61), V63 (= V62), N89 (= N91), A90 (= A92), I92 (≠ V94), V139 (≠ I140), S141 (= S142), Y154 (= Y155), K158 (≠ N159), P184 (= P191), G185 (≠ A192), I187 (vs. gap), T189 (= T195), M191 (≠ I197)

3osuA Crystal structure of the 3-oxoacyl-acyl carrier protein reductase, fabg, from staphylococcus aureus
33% identity, 93% coverage: 8:251/262 of query aligns to 5:243/246 of 3osuA

query
sites
3osuA

K

K

V

S

I

A

V

L

I

V

T

T

G

G

A

A

A

S

K

R

G
|
G

I

I

G

G

R

R

S

S

I

I

A

A

E

L

V

Q

F

L

A

A

K

E

E

E

N

G

A

Y

-

N

I

V

A

A

V

V

I

N

V

Y

G

A

R

G

K

S

A

K

E

E

D

K

N

A

Q

E

I

A

V

V

D

E

A

E

I

I

A

K

T

A

N

K

G

G

G

V

K

D

A

S

A

F

Q

A

F

I

V

Q

A

A

E

N

L

V

S

A

N

D

P

A

E

D

D

E

C

V

E

K

T

A

V

M

V

I

K

K

N

E

I

V

V

V

A

S

Q

Q

F

F

G

G

R

S

I

L

D

D

G

V

L

L

V

V

N

N

N
|
N

A

A

G

G

V

I

N

T

D

R

G

D

V

N

G

L

L

L

E

M

S

R

G

M

N

K

Y

E

K

Q

D

E

F

W

M

D

A

D

S

V

L

I

H

D

K

T

N

N

V

L

V

K

H

G

Y

V

Y

F

L

N

M

C

A

I

H

Q

H

K

V

A

L

T

P

P

E

Q

L

M

I

L

K

R

S

Q

K

R

-

S

G

G

A

A

I

I

V

I

N

N

I

L

T

S

S
|
S

K

V

T

V

A

G

E

A

T

V

G

G

Q

N

G

P

N

G

T
x
Q

S

A

A

N

Y
|
Y

A

V

A

A

A

T

N
x
K

G

A

G

G

R

V

N

I

A

G

L

L

T

T

R

K

E

S

W

A

A

A

V

R

E

E

L

L

L

A

K

S

Y

R

G

G

I

I

R

T

V

V

N

N

A

A

V

V

V

-

A

-

E

-

C

-

W

-

T

A

P

P

A

G

Y

F

E

-

T

I

W

V

I

S

E

D

T

M

L

T

D

D

N

A

A

L

E

S

E

D

K

E

L

L

K

K

E

E

-

Q

I

M

T

L

A

T

K

Q

I

I

P

P

L

L

E

A

N

-

R

R

M

F

T

G

T

Q

A

D

E

T

E

D

I

I

A

A

N

N

M

T

T

V

A

A

F

F

L

L

M

A

S

S

S

D

K

K

S

A

S

K

H

Y

T

I

T

T

G

G

Q

Q

I

T

I

I

H

H

V

V

D

N

G

G

active site: G15 (= G18), S141 (= S142), Q151 (≠ T152), Y154 (= Y155), K158 (≠ N159)
binding magnesium ion: N89 (= N91), K158 (≠ N159)

P0AEK2 3-oxoacyl-[acyl-carrier-protein] reductase FabG; 3-ketoacyl-acyl carrier protein reductase; Beta-Ketoacyl-acyl carrier protein reductase; Beta-ketoacyl-ACP reductase; EC 1.1.1.100 from Escherichia coli (strain K12) (see 2 papers)
30% identity, 95% coverage: 3:251/262 of query aligns to 1:240/244 of P0AEK2

query
sites
P0AEK2

L

M

Q

N

L

F

K

E

E

G

K

K

V

I

I

A

V

L

I

V

T

T

G
|
G

A
|
A

A
x
S

K
x
R

G

G

I

I

G

G

R

R

S

A

I

I

A

A

E

E

V

T

F

L

A

A

K

A

E

R

N

G

A

A

I

-

A

-

V

K

I

V

V

I

G

G

R

T

K

A

A
x
T

E

S

D

E

N

N

-

G

-

A

Q

Q

I

A

V

I

V

S

D

D

A

Y

I

L

A

G

T

A

N

N

G

G

G

-

K

-

A

-

A

K

Q

G

F

L

V

M

A

L

E
x
N

L
x
V

S

T

N

D

P

P

E

A

D

S

C

I

E

E

T

S

V

V

V

L

K

E

N

K

I

I

V

R

A

A

Q

E

F

F

G

G

R

E

I

V

D

D

G

I

L

L

V

V

N

N

N
|
N

A

A

G

G

V

I

N

T

D

R

G

D

V

N

G

L

L

L

E

M

S

R

G

M

N

K

Y

D

K

E

D

E

F

W

M

N

A

D

S

I

L

I

H

E

K

T

N

N

V

L

V

S

H

S

Y

V

Y

F

L

R

M

L

A

S

H

K

H

A

V

V

L

M

P

R

E

A

L

M

I

M

K

K

S

K

K

R

-

H

G

G

A

R

I

I

V

I

N

T

I

I

T

G

S

S

K

V

T

V

A

G

E

T

T

M

G

G

Q

N

G

G

N

G

T

Q

S

A

A

N

Y
|
Y

A
|
A

N
x
K

G

A

G

G

R

L

N

I

A

G

L

F

T

S

R

K

E

S

W

L

A

A

V

R

E

E

L

V

L

A

K

S

Y

R

G

G

I

I

R

T

V

V

N

N

A

V

V

V

V

-

A

-

E

-

C

-

W

-

T

A

P

P

A

G

Y

F

E
x
I

T

E

W

T

I

D

E

M

T

T

L

R

D

A

N

L

A

S

E

D

K

Q

L

R

K

A

E

G

I

I

T

L

A

A

K

Q

I

V

P

P

L

-

E

A

N

G

R

R

M

L

T

G

A

A

E

Q

E

E

I

I

A

A

N

N

M

A

T

V

A

A

F

F

L

L

M

A

S

S

S

D

K

E

S

A

H

Y

T

I

T

T

G

G

Q
x
E

I

T

I

L

H

H

V

V

D

N

G

G

GASR 12:15 (≠ GAAK 14:17) binding
T37 (≠ A41) binding
NV 59:60 (≠ EL 64:65) binding
N86 (= N91) binding
Y151 (= Y155) mutation to F: Defect in the affinity for NADPH.
YAAAK 151:155 (≠ YAAAN 155:159) binding
A154 (= A158) mutation to T: Decreases in the thermolability of the reductase; when associated with K-233.
K155 (≠ N159) mutation to A: Defect in the affinity for NADPH.
I184 (≠ E194) binding
E233 (≠ Q244) mutation to K: Decreases in the thermolability of the reductase; when associated with T-154.

Query Sequence

>CA265_RS08605 FitnessBrowser__Pedo557:CA265_RS08605
MDLQLKEKVIVITGAAKGIGRSIAEVFAKENAIAVIVGRKAEDNQIVVDAIATNGGKAAQ
FVAELSNPEDCETVVKNIVAQFGRIDGLVNNAGVNDGVGLESGNYKDFMASLHKNVVHYY
LMAHHVLPELIKSKGAIVNITSKTAETGQGNTSAYAAANGGRNALTREWAVELLKYGIRV
NAVVVAECWTPAYETWIETLDNAEEKLKEITAKIPLENRMTTAEEIANMTAFLMSSKSSH
TTGQIIHVDGGYVHLDRALANA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory