SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing CA265_RS08610 CA265_RS08610 alcohol dehydrogenase to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

4ilkA Crystal structure of short chain alcohol dehydrogenase (rspb) from e. Coli cft073 (efi target efi-506413) complexed with cofactor nadh
32% identity, 94% coverage: 1:318/339 of query aligns to 4:318/337 of 4ilkA

query
sites
4ilkA

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active site: C40 (= C37), G41 (= G38), S42 (≠ T39), H45 (= H42), H59 (= H59), E60 (= E60), C89 (= C88), C92 (= C91), C95 (= C94), C103 (= C102), A107 (≠ Q106), P145 (= P143), A149 (≠ G147)
binding manganese (ii) ion: C40 (= C37), H59 (= H59), E60 (= E60), E144 (= E142)
binding 1,4-dihydronicotinamide adenine dinucleotide: I148 (= I146), Y167 (≠ I165), G168 (= G166), G170 (= G168), P171 (= P169), I172 (= I170), A192 (≠ L188), D193 (= D189), R194 (≠ I190), I195 (≠ N191), R198 (= R194), N213 (= N209), A235 (= A234), A236 (≠ T235), C237 (≠ G236), I241 (≠ A240), M258 (≠ I257), F260 (≠ L259), S282 (= S281), R283 (= R282), L284 (vs. gap)
binding zinc ion: C89 (= C88), C92 (= C91), C95 (= C94), C103 (= C102)

Sites not aligning to the query:

active site: 332

1e3jA Ketose reductase (sorbitol dehydrogenase) from silverleaf whitefly (see paper)
31% identity, 94% coverage: 20:336/339 of query aligns to 21:345/348 of 1e3jA

query
sites
1e3jA

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active site: C38 (= C37), G39 (= G38), S40 (≠ T39), H43 (= H42), H63 (= H59), E64 (= E60), C93 (= C88), C96 (= C91), C99 (= C94), C107 (= C102), T111 (≠ Q106), P150 (= P143), G154 (= G147), K341 (= K332)
binding phosphate ion: G173 (= G166), A174 (= A167), A196 (≠ D189), R197 (≠ I190), S198 (≠ N191), R201 (= R194)
binding zinc ion: C38 (= C37), H63 (= H59), E64 (= E60), C93 (= C88), C96 (= C91), C99 (= C94), C107 (= C102)

Q7SI09 L-arabinitol 4-dehydrogenase; LAD; EC 1.1.1.12 from Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) (see 2 papers)
31% identity, 93% coverage: 21:335/339 of query aligns to 37:359/363 of Q7SI09

query
sites
Q7SI09

E

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C53 (= C37) binding
F59 (≠ A43) mutation F->A,S,Y: No effect.
H78 (= H59) binding
E79 (= E60) binding
C108 (= C88) binding
C111 (= C91) binding
C114 (= C94) binding
C122 (= C102) binding
E163 (= E142) binding
PI 190:191 (= PI 169:170) binding
D211 (= D189) binding
DI 211:212 (= DI 189:190) mutation to SR: Alters cofactor specificity from NAD to NADP; when associated with T-348.
R216 (= R194) binding
I282 (= I257) binding
QYR 306:308 (≠ SRN 281:283) binding
S348 (≠ L324) mutation to T: Alters cofactor specificity from NAD to NADP; when associated with 211-SR-212.

3m6iA L-arabinitol 4-dehydrogenase (see paper)
31% identity, 93% coverage: 21:335/339 of query aligns to 33:355/358 of 3m6iA

query
sites
3m6iA

E

E

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I

T

T

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M

E

L

F

C

A

A

R

K

I

A

A

A

G

G

A

A

-

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I

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x
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S

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active site: C49 (= C37), G50 (= G38), S51 (≠ T39), H54 (= H42), H74 (= H59), E75 (= E60), C104 (= C88), C107 (= C91), C110 (= C94), C118 (= C102), D122 (≠ Q106), P160 (= P143), A164 (≠ G147), K352 (= K332)
binding nicotinamide-adenine-dinucleotide: C49 (= C37), G50 (= G38), S51 (≠ T39), V163 (≠ I146), G183 (= G166), G185 (= G168), P186 (= P169), I187 (= I170), T206 (≠ L188), D207 (= D189), I208 (= I190), R212 (= R194), V228 (= V208), C255 (≠ A234), T256 (= T235), V258 (≠ N237), S261 (≠ A240), I278 (= I257), G279 (= G258), V280 (≠ L259), Q302 (≠ S281), Y303 (≠ R282), R304 (≠ N283)
binding zinc ion: C49 (= C37), H74 (= H59), C104 (= C88), C107 (= C91), C110 (= C94), C118 (= C102)

Q96V44 L-arabinitol 4-dehydrogenase; LAD; EC 1.1.1.12 from Hypocrea jecorina (Trichoderma reesei) (see paper)
29% identity, 93% coverage: 21:335/339 of query aligns to 50:373/377 of Q96V44

query
sites
Q96V44

E

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DI 224:225 (= DI 189:190) mutation to SR: Alters cofactor specificity from NAD to NADP; when associated with T-362.
A362 (≠ L324) mutation to T: Alters cofactor specificity from NAD to NADP; when associated with 224-SR-225.

2dfvA Hyperthermophilic threonine dehydrogenase from pyrococcus horikoshii (see paper)
28% identity, 99% coverage: 1:335/339 of query aligns to 3:345/346 of 2dfvA

query
sites
2dfvA

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L

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F

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active site: C40 (= C37), G41 (= G38), T42 (= T39), H45 (= H42), H65 (= H59), E66 (= E60), C95 (= C88), C98 (= C91), C101 (= C94), C109 (= C102), K113 (≠ Q106), P151 (= P143), A155 (≠ G147), K340 (≠ V330)
binding nicotinamide-adenine-dinucleotide: N154 (≠ I146), G173 (= G166), G175 (= G168), P176 (= P169), L177 (≠ I170), S196 (≠ D189), E197 (≠ I190), P198 (≠ N191), R202 (≠ L195), P217 (≠ A210), F241 (≠ A234), S242 (≠ T235), G243 (= G236), A244 (≠ N237), A247 (= A240), L264 (≠ I257), G265 (= G258), L266 (= L259), I289 (≠ S280), T290 (≠ S281)
binding zinc ion: C95 (= C88), C98 (= C91), C101 (= C94), C109 (= C102)

O58389 L-threonine 3-dehydrogenase; L-ThrDH; TDH; L-threonine dehydrogenase; EC 1.1.1.103 from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) (see 2 papers)
28% identity, 99% coverage: 1:335/339 of query aligns to 5:347/348 of O58389

query
sites
O58389

M

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V

K

I

V

K

V

A

F

M

M

V

L

C42 (= C37) binding
T44 (= T39) mutation to A: Total loss of enzymatic activity.
H67 (= H59) binding
E68 (= E60) binding
C97 (= C88) binding
C100 (= C91) binding
C103 (= C94) binding
C111 (= C102) binding
E152 (= E142) mutation E->A,Q: Almost complete loss of enzymatic activity.; mutation to C: 120-fold decrease in catalytic efficiency.; mutation to D: Shows 3-fold higher turnover rate and reduced affinities toward L-threonine and NAD(+), compared to wild-type.; mutation to K: Total loss of enzymatic activity.
L179 (≠ I170) binding
E199 (≠ I190) binding ; mutation to A: Large decrease in affinity for NAD(+).
R204 (≠ L195) binding ; mutation to A: Large decrease in affinity for NAD(+).
LGL 266:268 (≠ IGL 257:259) binding
IT 291:292 (≠ SS 280:281) binding
R294 (≠ N283) mutation to A: 4000-fold decrease in catalytic efficiency.

5vm2A Crystal structure of eck1772, an oxidoreductase/dehydrogenase of unknown specificity involved in membrane biogenesis from escherichia coli
28% identity, 98% coverage: 9:339/339 of query aligns to 11:347/347 of 5vm2A

query
sites
5vm2A

P

P

G

G

T

T

F

M

E

K

Y

I

S

I

E

S

T

A

T

E

K

I

P

P

E

V

L

P

K

K

K

E

D

D

H

E

A

V

I

L

I

I

K

K

I

V

K

E

R

Y

I

V

G

G

I

I

C
|
C

G
|
G

T
x
S

D

D

L

V

H
|
H

A

G

F

F

E

E

G

-

T

S

Q

G

P

P

F

F

F

I

-

P

-

K

-

D

-

P

N

N

Y

Q

P

E

R

I

V

G

L

L

G

G

H
|
H

E
|
E

L

C

S

A

G

G

E

T

L

V

V

V

E

A

A

V

D

G

G

S

-

R

A

V

D

R

G

K

F

F

K

K

I

P

G

G

E

D

A

R

V

V

T

N

F

I

I

E

P

P

Y

G

F

V

N

P

C
|
C

G

G

E

H

C
|
C

I

R

A

Y

C
|
C

R

L

M

E

N

G

K

K

P

Y

N

N

C

I

C
|
C

-

P

-

D

V

V

K
x
D

M

F

Q

M

V

A

C

T

G

Q

V

P

H

N

V

Y

D

R

G

G

G

A

M

L

R

T

E

H

Y

Y

L

L

Q

C

V

H

P

P

-

E

S

S

R

F

T

T

L

Y

L

K

H

L

G

P

E

D

G

N

L

M

S

D

Y

T

D

M

E

E

L

G

A

T

L

L

V

V

E
|
E

P
|
P

L

A

A

A

I

V

G
|
G

A

M

H

H

G

A

V

A

R

M

R

L

A

A

D

D

V

V

Q

K

P

P

G

G

E

K

F

K

V

I

L

I

V

I

I

L

G

G

A

A

G

G

P

C

I

I

G

G

L

L

G

M

T

T

M

L

E

Q

F

A

A

C

R

K

I

C

A

L

G

G

A

A

N

T

V

E

I

I

A

A

-

V

L

V

D

D

I

V

N

L

E

E

D

K

R

R

L

L

A

A

F

M

C

A

K

-

D

E

K

Q

L

L

K

G

V

A

A

T

H

V

V

V

V

I

N

N

A

G

L

A

S

K

P

E

D

D

V

T

V

I

Q

A

Q

R

L

C

S

Q

D

Q

I

F

T

T

N

E

G

D

D

M

M

G

P

A

T

D

V

I

V

V

I

F

D

E

A

T

T

A

G

G

N

S

Q

A

K

V

A

T

I

V

N

K

N

Q

A

A

I

P

N

Y

Y

L

M

V

A

M

H

R

G

G

A

G

R

K

F

I

V

M

L

I

I

V

G

G

L

T

Q

V

K

P

G

G

D

A

L

S

I

A

F

I

N

N

H

F

P

L

E

K

F

I

H

N

K

R

R

E

E

V

S

T

T

I

L

Q

M

T

S

V

S

F

R

R

N

Y

A

A

T

N

I

-

E

-

D

R

F

Y

E

P

H

V

V

T

I

I

K

E

S

A

M

I

K

S

A

S

G

G

L

R

V

F

N

D

P

V

T

K

N

S

Y

M

I

V

T

T

H

H

Q

I

V

Y

Q

D

F

Y

E

R

A

D

V

V

K

Q

D

Q

E

A

F

F

E

E

S

E

W

S

L

V

D

N

P

N

K

K

N

R

G

D

V

I

I

I

K
|
K

A

G

M

V

V

I

S

K

L

I

G

S

N

D

active site: C39 (= C37), G40 (= G38), S41 (≠ T39), H44 (= H42), H65 (= H59), E66 (= E60), C95 (= C88), C98 (= C91), C101 (= C94), C109 (= C102), D113 (≠ K104), P153 (= P143), G157 (= G147), K340 (= K332)
binding magnesium ion: H65 (= H59), E66 (= E60), E152 (= E142)
binding zinc ion: C95 (= C88), C98 (= C91), C101 (= C94), C109 (= C102)

B6HI95 L-arabinitol 4-dehydrogenase; LAD; EC 1.1.1.12 from Penicillium rubens (strain ATCC 28089 / DSM 1075 / NRRL 1951 / Wisconsin 54-1255) (Penicillium chrysogenum) (see paper)
27% identity, 93% coverage: 22:335/339 of query aligns to 39:369/385 of B6HI95

query
sites
B6HI95

L

L

K

K

K

A

D

G

H

E

A

V

I

T

I

I

K

E

I

V

K

R

R

S

I

T

G

G

I

I

C

C

G

G

T

S

D

D

L

V

H

H

A

F

F

W

E

H

G

A

-

G

-

C

T

I

Q

G

P

P

F

M

F

I

-

V

N

T

Y

G

P

D

R

H

V

V

L

L

G

G

H

H

E

E

L

S

S

A

G

G

E

Q

-

V

L

L

V

A

E

V

A

A

D

P

G

D

A

V

D

T

G

H

F

L

K

K

I

V

G

G

E

D

A

R

V

V

T

A

F

V

I

E

P

P

Y

N

F

V

N

I

C

C

G

N

E

A

C

C

I

E

A

P

C

C

R

L

M

T

N

G

K

R

P

Y

N

N

C

G

C

C

V

V

K

N

M

V

Q

A

V

F

C

L

G

S

V

T

H

P

-

P

V

V

D

D

G

G

G

L

M

L

R

R

E

R

Y

Y

L

V

Q

N

V

H

P

P

S

A

R

V

T

W

L

C

L

H

H

K

G

I

E

G

G

D

L

M

S

S

Y

Y

D

E

E

D

L

G

A

A

L

M

V

L

E

E

P

P

L

L

A

S

I

V

G

T

A

L

H

A

G

A

V

I

R

E

R

R

A

S

D

G

V

L

Q

R

P

L

G

G

E

D

F

P

V

L

L

L

V

I

I

T

G

G

A

A

G

G

P

P

I

I

G

G

L

L

G

I

T

S

M

L

E

L

F

S

A

A

R

R

I

A

A

A

G

G

A

A

-

C

N

P

V

I

I

V

A

I

L

T

D
|
D

I
|
I

N

D

E

E

D

G

R

R

L

L

A

A

F

F

C

A

K

K

D

S

-

L

-

V

-

P

-

E

-

V

-

R

-

T

-

Y

K

K

L

V

K

E

V

I

A

G

H

K

V

S

V

A

N

E

A

E

L

C

S

A

P

D

D

G

V

I

Q

N

Q

A

L

L

S

N

D

D

I

G

T

Q

N

G

G

S

D

G

-

P

-

D

-

A

-

L

M

R

P

P

T

K

V

L

V

A

I

L

D

E

A

C

T

T

G

G

N

V

Q

E

K

S

A

S

I

V

N

N

N

S

A

A

I

I

N

W

Y

S

M

V

A

K

H

F

G

G

A

G

R

K

F

V

V

F

L

V

I

I

G

G

L

V

Q

G

K

K

G

N

D

E

L

M

I

T

F

I

N

P

H

F

P

M

E

R

F

L

H

S

K

T

R

Q

E

E

S

I

T

D

L

L

M

Q

S

Y

S

Q

R

Y

N

R

A

-

T

Y

I

C

E

N

D

T

F

W

E

P

H

R

V

A

I

I

K

R

S

L

M

I

K

Q

A

N

G

G

L

V

V

I

N

D

P

L

T

S

N

K

Y

L

I

V

T

T

H

H

Q

R

V

Y

Q

S

F

L

E

E

A

N

V

A

K

L

D

Q

E

A

F

F

E

E

S

T

W

A

L
x
S

D

N

P

P

K

K

N

T

G

G

V

A

I

I

K

K

A

V

M

Q

V

I

DI 212:213 (= DI 189:190) mutation to SR: Alters cofactor specificity from NAD to NADP; when associated with T-358.
S358 (≠ L324) mutation to T: Alters cofactor specificity from NAD to NADP; when associated with 212-SR-213.

7y9pA Xylitol dehydrogenase s96c/s99c/y102c mutant(thermostabilized form) from pichia stipitis (see paper)
28% identity, 96% coverage: 11:335/339 of query aligns to 18:354/357 of 7y9pA

query
sites
7y9pA

T

T

F

Y

E

D

Y

A

S

P

E

E

T

I

T

S

K

E

P

P

E

T

L

-

K

-

D

-

H

D

A

V

I

L

I

V

K

Q

I

V

K

K

R

K

I

T

G

G

I

I

C
|
C

G

G

T

S

D

D

L

I

H

H

A

F

F

Y

E

A

G

H

T

G

Q

R

P

I

-

G

-

N

-

F

F

V

F

L

N

T

Y

K

P

P

R

M

V

V

L

L

G

G

H
|
H

E
|
E

L

S

S

A

G

G

E

T

L

V

V

V

E

Q

-

V

A

G

D

K

G

G

A

V

D

T

G

S

F

L

K

K

I

V

G

G

E

D

A

N

V

V

T

A

F

I

I

E

P

P

Y

G

F

I

N

P

C
|
C

G

R

E

F

C
|
C

I

D

A

E

C
|
C

R

K

M

S

N

G

K

H

P

Y

N

N

C

L

C
|
C

V

P

K

H

M

M

Q

A

V

F

C

A

G

A

V

T

-

P

-

N

-

E

-

P

H

N

V

P

D

P

G

G

G

T

M

L

R

C

E

K

Y

Y

L

F

Q

K

V

S

P

P

S

E

R

D

T

F

L

L

L

V

H

K

-

L

G

P

E

D

G

H

L

V

S

S

Y

L

D

E

E

L

L

G

A

A

L

L

V

V

E

E

P

P

L

L

A

S

I

V

G

G

A

V

H

H

G

A

V

S

R

K

R

L

A

G

D

S

V

V

Q

A

P

F

G

G

E

D

F

Y

V

V

L

A

V

V

I

F

G

G

A

A

G

G

P

P

I

V

G

G

L

L

G

L

T

A

M

A

E

A

F

V

A

A

R

K

I

T

A

F

G

G

A

A

N

K

-

G

V

V

I

I

A

V

L

V

D

D

I

I

N

F

E

D

D

N

R

K

L

L

A

K

F

M

C

A

K

K

D

D

K

I

L

G

K

A

V

A

A

T

H

H

V

T

V

F

N

N

A

S

L

K

S

T

P

G

D

G

V

-

V

S

Q

E

L

L

S

I

D

K

I

A

T

F

N

G

G

G

D

N

M

V

P

P

T

N

V

V

I

L

D

E

A

C

T

T

G

G

N

A

Q

E

K

P

A

C

I

I

N

K

N

L

A

G

I

V

N

D

Y

A

M

I

A

A

H

P

G

G

A

G

R

R

F

F

V

V

L

Q

I

V

G

G

L

N

Q

A

K

A

G

G

D

P

L

V

I

S

F

F

N

P

H

I

P

T

E

V

F

F

H

A

K

M

R

K

E

E

S

L

T

T

L

L

M

F

S

G

S

S

R

F

N

R

A

Y

T

G

I

F

E

N

D

D

F

Y

E

K

H

T

V

A

I

V

K

G

S

I

M

F

K

D

A

T

G

N

L

Y

V

Q

N

N

-

G

-

R

-

E

-

N

-

A

P

P

T

I

N

D

Y

F

-

E

-

Q

-

L

I

I

T

T

H

H

Q

R

V

Y

Q

K

F

F

E

-

A

-

V

-

K

K

D

D

E

A

F

I

E

E

S

A

W

Y

-

D

-

L

L

V

D

R

P

A

K

G

N

K

G

G

V

A

I

V

K

K

A

C

M

L

V

I

binding zinc ion: C40 (= C37), H65 (= H59), E66 (= E60), C95 (= C88), C98 (= C91), C101 (= C94), C109 (= C102)

2eerB Structural study of project id st2577 from sulfolobus tokodaii strain7
29% identity, 89% coverage: 20:322/339 of query aligns to 21:333/347 of 2eerB

query
sites
2eerB

P

P

E

K

L

P

K

K

K

G

D

S

H

Q

A

V

I

L

I

I

K

K

I

I

K

E

R

A

I

A

G

G

I

V

C
|
C

G
x
H

T
x
S

D

D

L

V

H
|
H

A

M

F

R

E

Q

G

G

T

R

Q

-

P

-

F

F

F

G

N

N

Y

L

-

R

-

I

-

V

-

E

-

D

-

L

-

G

-

V

-

K

-

L

P

P

R

V

V

T

L

L

G

G

H
|
H

E
|
E

L

I

S

A

G

G

E

R

L

I

V

E

E

E

-

V

A

G

D

D

G

E

A

V

D

V

G

G

F

Y

K

S

I

K

G

G

E

D

A

L

V

V

T

A

F

V

I

N

P

P

Y

W

F

E

N

G

C
x
E

G

G

E

N

C
|
C

I

Y

A

Y

C
|
C

R

R

M

I

N

G

K

E

P

E

N

H

C

L

C
|
C

V

D

K

S

M

P

Q
x
R

V

W

C

L

G

G

V

I

H

N

V

Y

D

D

G

G

G

A

M

Y

R

A

E

E

Y

Y

L

V

Q

L

V

V

P

P

S

H

R

Y

T

K

L

Y

L

L

H

Y

G

-

E

-

G

-

L

-

S

K

Y

L

D

R

E

R

L

L

A

S

L

A

V

V

E

E

P

A

L

A

A

P

I

L

G

T

A
x
C

H

S

G

G

V

V

-
x
T

-

T

-

Y

-

R

-

A

-

V

R

R

R

K

A

A

D

S

V

L

Q

D

P

P

G

S

E

K

F

T

V

L

L

V

V

V

I
|
I

G
|
G

A

A

G
|
G

P
x
G

I

-

G
|
G

L
|
L

G

G

T

T

M

M

-

A

-

I

E

Q

F

I

A

A

R

K

-

A

I

V

A

S

G

G

A

A

N

T

V

I

I

I

A

G

L
x
V

D
|
D

I
x
V

N
x
R

E

E

D

E

R

A

L

L

A

E

F

A

C

A

K

K

D

-

K

R

L

A

K

G

V

A

A

D

H

Y

V

V

V

I

N

N

A

A

L

S

S

S

P

Q

D

D

V

P

V

V

Q

S

Q

E

L

I

S

R

D

R

I

I

T

T

N

Q

G

G

D

K

M

G

P

A

T

D

V

A

V

V

I

I

D

D

A
x
L

T
x
N

G

N

N

S

Q

E

K
|
K

A
x
T

I

L

N

S

N

I

A

Y

I

P

N

Y

Y

V

M

L

A

A

H

K

G

Q

A

G

R

K

F

Y

V

V

L

M

I
x
V

G
|
G

L
|
L

Q
x
F

K

G

G

A

D

D

L

L

I

K

F

Y

N

H

H

A

P

P

E

L

F

I

H

T

K

L

R

N

E

E

S

V

T

Q

L

F

M

I

S

G

S
|
S

R
x
L

N
x
V

A

G

T

N

I

Q

E

S

D

D

F

F

E

L

H

G

V

I

I

M

K

S

S

L

M

A

K

E

A

A

G

G

L

K

V

V

N

K

P

P

T

M

N

-

Y

-

I

V

T

T

H

K

Q

T

V

M

Q

K

F

L

E

E

A

E

V

A

K

N

D

E

E

A

F

I

E

D

S

N

active site: C38 (= C37), H39 (≠ G38), S40 (≠ T39), H43 (= H42), H68 (= H59), E69 (= E60), E98 (≠ C88), C101 (= C91), C104 (= C94), C112 (= C102), R116 (≠ Q106), C154 (≠ A148), T158 (vs. gap)
binding nicotinamide-adenine-dinucleotide: C38 (= C37), H39 (≠ G38), S40 (≠ T39), H43 (= H42), C154 (≠ A148), T158 (vs. gap), I177 (= I165), G178 (= G166), G180 (= G168), G181 (≠ P169), G182 (= G171), L183 (= L172), V202 (≠ L188), D203 (= D189), V204 (≠ I190), R205 (≠ N191), L247 (≠ A234), N248 (≠ T235), K252 (= K239), T253 (≠ A240), V270 (≠ I257), G271 (= G258), L272 (= L259), F273 (≠ Q260), S294 (= S281), L295 (≠ R282), V296 (≠ N283)
binding zinc ion: E98 (≠ C88), C101 (= C91), C104 (= C94), C112 (= C102)

Sites not aligning to the query:

active site: 342
binding nicotinamide-adenine-dinucleotide: 334, 337, 342

3gfbA L-threonine dehydrogenase (tktdh) from the hyperthermophilic archaeon thermococcus kodakaraensis (see paper)
29% identity, 95% coverage: 1:321/339 of query aligns to 3:331/347 of 3gfbA

query
sites
3gfbA

M

M

K

Q

T

A

L

I

T

M

C

K

T

T

T

K

P

P

G

A

T

Y

-

G

F

A

E

E

Y

L

S

V

E

E

T

V

T

D

K

V

P

P

E

K

L

P

K

G

K

P

D

G

H

E

A

V

I

L

I

I

K

K

I

V

K

L

R

A

I

T

G

S

I

I

C
|
C

G
|
G

T
|
T

D

D

L

L

H
|
H

A

I

F

Y

E

E

G

W

-

N

-

E

-

W

T

A

Q

Q

P

S

F

R

F

I

N

K

Y

P

P

P

R

Q

V

I

L

M

G

G

H
|
H

E
|
E

L

V

S

A

G

G

E

E

L

V

V

V

E

E

-

V

A

G

D

P

G

G

A

V

D

E

G

D

F

L

K

Q

I

V

G

G

E

D

A

Y

V

I

T

S

F

V

I

E

P

T

Y

H

F

I

N

V

C
|
C

G

G

E

K

C
|
C

I

Y

A

A

C
|
C

R

K

M

H

N

N

K

R

P

Y

N

H

C

V

C
|
C

V

Q

K

N

M

T

Q
x
K

V

I

C

F

G

G

V

V

H

D

V

M

D

D

G

G

G

V

M

F

R

A

E

H

Y

Y

L

A

Q

I

V

V

P

P

S

A

R

K

T

N

L

A

L

W

H

K

G

N

-

P

E

K

G

D

L

M

S

P

Y

P

D

E

E

Y

L

A

A

A

L

L

V

Q

E

E

P
|
P

L

L

A

G

I

N

G
x
A

A

V

H

D

G

T

V

V

R

L

R

A

A

G

D

P

V

I

Q

A

P

-

G

G

E

R

F

S

V

T

L

L

V

I

I

T

G
|
G

A
|
A

G
|
G

P
|
P

I
x
L

G

G

L

L

G

L

T

G

M

I

E

A

F

V

A

A

R

K

I

A

A

S

G

G

A

A

-

Y

N

P

V

V

I

I

A

V

L
x
S

D
x
E

I
x
P

N

S

E

E

D

F

R
|
R

L

R

A

K

F

L

C

A

K

K

D

-

K

K

L

V

K

G

V

A

A

D

H

Y

V

V

N

N

A
x
P

L

F

S

E

P

E

D

D

V

P

V

V

Q

K

Q

F

L

V

S

M

D

D

I

I

T

T

N

D

G

G

D

A

M

G

P

V

T

E

V

V

V

F

I

L

D

E

A
x
F

T
x
S

G

G

N
x
A

Q

P

K

K

A
|
A

I

L

N

E

N

Q

A

G

I

L

N

K

Y

A

M

V

A

T

H

P

G

G

A

G

R

R

F

V

V

S

L

L

I
x
L

G
|
G

L
|
L

-

F

-

P

Q

R

K

E

G

V

D

T

L

I

I

D

F

F

N

N

H

N

P

L

E

I

F
x
I

H
x
F

K

K

R

A

E

L

S

E

T

V

L

H

M

G

S
x
I

S
x
T

R

G

N

R

A

H

T

L

I

W

E

E

D

T

F

W

E

Y

H

T

V

V

I

S

K

S

S

L

M

I

K

Q

A

S

G

G

L

K

V

L

N

N

P

L

T

D

N

P

Y

I

I

I

T

T

H

H

Q

K

V

Y

Q

K

-

G

F

F

E

D

A

K

V

F

K

E

D

E

E

A

F

F

E

E

active site: C40 (= C37), G41 (= G38), T42 (= T39), H45 (= H42), H65 (= H59), E66 (= E60), C95 (= C88), C98 (= C91), C101 (= C94), C109 (= C102), K113 (≠ Q106), P151 (= P143), A155 (≠ G147)
binding nicotinamide-adenine-dinucleotide: G173 (= G166), A174 (= A167), G175 (= G168), P176 (= P169), L177 (≠ I170), S196 (≠ L188), E197 (≠ D189), P198 (≠ I190), R202 (= R194), P217 (≠ A210), F241 (≠ A234), S242 (≠ T235), A244 (≠ N237), A247 (= A240), L264 (≠ I257), G265 (= G258), L266 (= L259), I280 (≠ F271), F281 (≠ H272), I289 (≠ S280), T290 (≠ S281)

Sites not aligning to the query:

active site: 340

Q5JI69 L-threonine 3-dehydrogenase; TDH; L-threonine dehydrogenase; EC 1.1.1.103 from Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1)) (see paper)
29% identity, 95% coverage: 1:321/339 of query aligns to 5:333/350 of Q5JI69

query
sites
Q5JI69

M

M

K

Q

T

A

L

I

T

M

C

K

T

T

T

K

P

P

G

A

T

Y

-

G

F

A

E

E

Y

L

S

V

E

E

T

V

T

D

K

V

P

P

E

K

L

P

K

G

K

P

D

G

H

E

A

V

I

L

I

I

K

K

I

V

K

L

R

A

I

T

G

S

I

I

C

C

G

G

T

T

D

D

L

L

H

H

A

I

F

Y

E

E

G

W

-

N

-

E

-

W

T

A

Q

Q

P

S

F

R

F

I

N

K

Y

P

P

P

R

Q

V

I

L

M

G

G

H

H

E

E

L

V

S

A

G

G

E

E

L

V

V

V

E

E

-

V

A

G

D

P

G

G

A

V

D

E

G

D

F

L

K

Q

I

V

G

G

E

D

A

Y

V

I

T

S

F

V

I

E

P

T

Y

H

F

I

N

V

C

C

G

G

E

K

C

C

I

Y

A

A

C

C

R

K

M

H

N

N

K

R

P

Y

N

H

C

V

C

C

V

Q

K

N

M

T

Q

K

V

I

C

F

G

G

V

V

H

D

V

M

D

D

G

G

G

V

M

F

R

A

E

H

Y

Y

L

A

Q

I

V

V

P

P

S

A

R

K

T

N

L

A

L

W

H

K

G

N

-

P

E

K

G

D

L

M

S

P

Y

P

D

E

E

Y

L

A

A

A

L

L

V

Q

E

E

P

P

L

L

A

G

I

N

G

A

A

V

H

D

G

T

V

V

R

L

R

A

A

G

D

P

V

I

Q

A

P

-

G

G

E

R

F

S

V

T

L

L

V

I

I

T

G

G

A

A

G

G

P

P

I
x
L

G

G

L

L

G

L

T

G

M

I

E

A

F

V

A

A

R

K

I

A

A

S