SitesBLAST
Comparing CA265_RS08650 CA265_RS08650 short-chain dehydrogenase to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
4bmsF Short chain alcohol dehydrogenase from ralstonia sp. Dsm 6428 in complex with NADPH
38% identity, 99% coverage: 1:245/248 of query aligns to 1:247/249 of 4bmsF
- active site: S137 (= S135), H147 (≠ S145), Y150 (= Y148), K154 (= K152), Q195 (≠ E193)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G13 (= G13), N15 (= N15), S16 (= S16), I18 (= I18), R38 (= R38), R39 (= R39), A59 (= A57), D60 (= D58), V61 (≠ Q59), N87 (= N85), S88 (≠ A86), G89 (= G87), V110 (≠ I108), S137 (= S135), Y150 (= Y148), K154 (= K152), G181 (= G179), I183 (= I181), T185 (= T183), I187 (= I185)
6ihhA Crystal structure of rasadh f12 from ralstonia.Sp in complex with NADPH and a6o
38% identity, 99% coverage: 1:245/248 of query aligns to 1:247/249 of 6ihhA
- binding (2R,3S)-2-ethyl-2-[(2E)-2-(6-methoxy-3,4-dihydro-2H-naphthalen-1-ylidene)ethyl]-3-oxidanyl-cyclopentan-1-one: S137 (= S135), H147 (≠ S145), Y150 (= Y148), L188 (≠ M186), L246 (≠ M244)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G13 (= G13), N15 (= N15), S16 (= S16), G17 (= G17), I18 (= I18), R38 (= R38), R39 (= R39), D60 (= D58), V61 (≠ Q59), N87 (= N85), S88 (≠ A86), G89 (= G87), V110 (≠ I108), T135 (≠ L133), S137 (= S135), Y150 (= Y148), K154 (= K152), P180 (= P178), G181 (= G179), A182 (≠ P180), I183 (= I181), T185 (= T183), S187 (≠ I185)
4esoB Crystal structure of a putative oxidoreductase protein from sinorhizobium meliloti 1021 in complex with NADP
38% identity, 99% coverage: 3:247/248 of query aligns to 2:247/251 of 4esoB
- active site: G16 (= G17), S136 (= S135), M146 (≠ S145), Y149 (= Y148), K153 (= K152)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G13), T14 (≠ N15), H15 (≠ S16), M17 (≠ I18), R37 (= R38), N38 (≠ R39), N41 (≠ A42), S58 (≠ A57), D59 (= D58), I60 (≠ Q59), N86 (= N85), A87 (= A86), G88 (= G87), T134 (≠ L133), S136 (= S135), Y149 (= Y148), P179 (= P178), G180 (= G179), I182 (= I181), T184 (= T183), T186 (≠ I185), K187 (≠ M186), G188 (≠ N187)
P50162 Tropinone reductase 1; Tropine dehydrogenase; Tropinone reductase I; TR-I; EC 1.1.1.206 from Datura stramonium (Jimsonweed) (Common thornapple) (see paper)
37% identity, 98% coverage: 4:245/248 of query aligns to 19:268/273 of P50162
- 25:49 (vs. 10:34, 60% identical) binding
- S158 (= S135) binding
- Y171 (= Y148) active site, Proton acceptor
1ae1B Tropinone reductase-i complex with NADP (see paper)
37% identity, 98% coverage: 3:245/248 of query aligns to 3:253/258 of 1ae1B
- active site: G17 (= G17), S143 (= S135), V153 (≠ S145), Y156 (= Y148), K160 (= K152)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G13 (= G13), S15 (≠ N15), K16 (≠ S16), G17 (= G17), I18 (= I18), S37 (≠ G37), R38 (= R38), C62 (≠ A57), D63 (= D58), L64 (≠ Q59), N91 (= N85), A92 (= A86), S143 (= S135), Y156 (= Y148), K160 (= K152), P186 (= P178), I189 (= I181), T191 (= T183), L193 (≠ I185), V194 (≠ M186)
5fffA Noroxomaritidine/norcraugsodine reductase in complex with NADP+ and piperonal (see paper)
36% identity, 98% coverage: 3:246/248 of query aligns to 8:255/257 of 5fffA
- active site: K206 (= K188)
- binding 1,3-benzodioxole-5-carbaldehyde: Y100 (≠ V89), H158 (≠ S145)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G18 (= G13), T20 (≠ N15), G22 (= G17), I23 (= I18), R43 (= R38), C67 (≠ A57), D68 (= D58), V69 (≠ Q59), N96 (= N85), I146 (≠ L133), Y161 (= Y148), K165 (= K152), P191 (= P178), A193 (≠ P180), I194 (= I181), T196 (= T183), G198 (vs. gap), T199 (vs. gap)
5ff9B Noroxomaritidine/norcraugsodine reductase in complex with NADP+ and tyramine (see paper)
36% identity, 98% coverage: 3:246/248 of query aligns to 8:255/257 of 5ff9B
- active site: K206 (= K188)
- binding 4-(2-aminoethyl)phenol: Y100 (≠ V89), I155 (≠ G142), H158 (≠ S145)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G18 (= G13), T20 (≠ N15), K21 (≠ S16), I23 (= I18), S42 (≠ G37), R43 (= R38), C67 (≠ A57), D68 (= D58), V69 (≠ Q59), N96 (= N85), I146 (≠ L133), S148 (= S135), Y161 (= Y148), K165 (= K152), P191 (= P178), A193 (≠ P180), I194 (= I181), T196 (= T183), G198 (vs. gap), T199 (vs. gap)
A0A1A9TAK5 Noroxomaritidine/norcraugsodine reductase; NorRed; EC 1.1.1.- from Narcissus pseudonarcissus (Daffodil) (see paper)
36% identity, 98% coverage: 3:246/248 of query aligns to 8:255/257 of A0A1A9TAK5
P50163 Tropinone reductase 2; Tropinone reductase II; TR-II; EC 1.1.1.236 from Datura stramonium (Jimsonweed) (Common thornapple) (see paper)
34% identity, 98% coverage: 3:244/248 of query aligns to 6:254/260 of P50163
- 18:41 (vs. 15:38, 42% identical) binding
- S146 (= S135) binding
- IATSL 192:196 (≠ IATEI 181:185) binding
2ae2A Tropinone reductase-ii complexed with NADP+ and pseudotropine (see paper)
34% identity, 98% coverage: 3:244/248 of query aligns to 5:253/259 of 2ae2A
- active site: G19 (= G17), S145 (= S135), Y158 (= Y148), K162 (= K152)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G15 (= G13), S17 (≠ N15), R18 (≠ S16), G19 (= G17), I20 (= I18), S39 (≠ G37), R40 (= R38), C64 (≠ A57), L66 (≠ Q59), N93 (= N85), G95 (= G87), I116 (= I108), I143 (≠ L133), S145 (= S135), Y158 (= Y148), K162 (= K152), P188 (= P178), G189 (= G179), V190 (≠ P180), I191 (= I181), T193 (= T183), S194 (≠ E184), L195 (≠ I185), V196 (≠ M186)
- binding pseudotropine: S145 (= S135), E155 (≠ S145), Y158 (= Y148), L195 (≠ I185)
1ipfA Tropinone reductase-ii complexed with NADPH and tropinone (see paper)
34% identity, 98% coverage: 3:244/248 of query aligns to 5:253/259 of 1ipfA
- active site: G19 (= G17), S145 (= S135), Y158 (= Y148), K162 (= K152)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G15 (= G13), R18 (≠ S16), G19 (= G17), I20 (= I18), S39 (≠ G37), R40 (= R38), C64 (≠ A57), D65 (= D58), L66 (≠ Q59), N93 (= N85), S145 (= S135), Y158 (= Y148), K162 (= K152), P188 (= P178), V190 (≠ P180), I191 (= I181), T193 (= T183), S194 (≠ E184), L195 (≠ I185), V196 (≠ M186)
- binding 8-methyl-8-azabicyclo[3,2,1]octan-3-one: S147 (≠ T137), E155 (≠ S145), Y158 (= Y148)
1ipeA Tropinone reductase-ii complexed with NADPH (see paper)
34% identity, 98% coverage: 3:244/248 of query aligns to 5:253/259 of 1ipeA
- active site: G19 (= G17), S145 (= S135), Y158 (= Y148), K162 (= K152)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G15 (= G13), S17 (≠ N15), R18 (≠ S16), G19 (= G17), I20 (= I18), S39 (≠ G37), R40 (= R38), C64 (≠ A57), D65 (= D58), L66 (≠ Q59), N93 (= N85), I116 (= I108), S145 (= S135), Y158 (= Y148), K162 (= K152), P188 (= P178), I191 (= I181), T193 (= T183), S194 (≠ E184), L195 (≠ I185), V196 (≠ M186)
7do7A Crystal structure of azotobacter vinelandii l-rhamnose 1- dehydrogenase(NAD and l-rhamnose bound-form) (see paper)
33% identity, 98% coverage: 2:244/248 of query aligns to 1:251/256 of 7do7A
- active site: G16 (= G17), S146 (= S135), Y159 (= Y148)
- binding nicotinamide-adenine-dinucleotide: G12 (= G13), R15 (≠ S16), G16 (= G17), I17 (= I18), S37 (≠ R38), D66 (= D58), A67 (≠ Q59), N93 (= N85), A94 (= A86), G95 (= G87), I96 (≠ V88), V144 (≠ L133), S145 (= S134), S146 (= S135), Y159 (= Y148), K163 (= K152), P189 (= P178), G190 (= G179), I192 (= I181), T194 (= T183), I196 (= I185)
- binding beta-L-rhamnopyranose: F99 (≠ Q91), S146 (= S135), S148 (≠ T137), Q156 (≠ S145), Y159 (= Y148), N197 (= N192), D235 (= D228), M236 (≠ A229), R238 (≠ T231)
7b81A Crystal structure of azotobacter vinelandii l-rhamnose 1-dehydrogenase (NAD bound-form) (see paper)
33% identity, 98% coverage: 2:244/248 of query aligns to 1:251/256 of 7b81A
- active site: G16 (= G17), S146 (= S135), Y159 (= Y148)
- binding nicotinamide-adenine-dinucleotide: G12 (= G13), S14 (≠ N15), R15 (≠ S16), I17 (= I18), D66 (= D58), A67 (≠ Q59), N93 (= N85), A94 (= A86), G95 (= G87), I96 (≠ V88), T116 (≠ I108), V144 (≠ L133), S146 (= S135), Y159 (= Y148), K163 (= K152), P189 (= P178), G190 (= G179), I192 (= I181), T194 (= T183), I196 (= I185)
3o4rA Crystal structure of human dehydrogenase/reductase (sdr family) member 4 (dhrs4)
35% identity, 97% coverage: 4:243/248 of query aligns to 6:249/254 of 3o4rA
- active site: G19 (= G17), S145 (= S135), F155 (≠ S145), Y158 (= Y148), K162 (= K152), K203 (≠ E193)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: A15 (≠ G13), T17 (≠ N15), D18 (≠ S16), G19 (= G17), I20 (= I18), S39 (≠ G37), R40 (= R38), K41 (≠ R39), N44 (≠ A42), H65 (≠ D58), V66 (≠ Q59), N92 (= N85), A94 (≠ G87), S145 (= S135), Y158 (= Y148), K162 (= K152), P188 (= P178), G189 (= G179), L190 (≠ P180), I191 (= I181), T193 (= T183), F195 (≠ I185), S196 (≠ M186)
Q9BTZ2 Dehydrogenase/reductase SDR family member 4; NADPH-dependent carbonyl reductase; CR; NADPH-dependent retinol dehydrogenase/reductase; NRDR; humNRDR; Peroxisomal short-chain alcohol dehydrogenase; PSCD; SCAD-SRL; Short chain dehydrogenase/reductase family 25C member 2; Protein SDR25C2; Short-chain dehydrogenase/reductase family member 4; EC 1.1.1.184 from Homo sapiens (Human) (see 2 papers)
35% identity, 97% coverage: 4:243/248 of query aligns to 30:273/278 of Q9BTZ2
- S176 (≠ G142) Responsible for the stereoselective reduction of 3-ketosteroids into 3beta-hydroxysteroids and benzil into R-benzoin; mutation to F: Decreased reduction activity for benzil, isatin and retinal and increased activity for 5beta-Pregnane-3,20-dione and 5beta-Dihydrotestosterone. No change of stereoselectivity in 3-ketosteroids reduction and no change in 3beta-hydroxysteroid oxidation. Decreased reduction activity for isatin and increased activity for 5beta-Pregnane-3,20-dione, 5beta-Dihydrotestosterone, benzil and retinal; when associated with L-179. Change in stereoselective activity by the reduction of 5beta-Pregnane-3,20-dione predominantly to the 3alpha-hydroxysteroid; when associated with L-179. Switch from 3beta-hydroxysteroid to 3alpha-hydroxysteroid oxidation; when associated with L-179. Loss of cold catalytic inactivation; when associated with L-179 and N-195. Increased reduction activity for renital and oxidation activity for retinol; when associated with L-179 and N-195.
- F179 (≠ S145) Responsible for the stereoselective reduction of 3-ketosteroids into 3beta-hydroxysteroids and benzil into R-benzoin; mutation to L: Decreased reduction activity for isatin and increased activity for 5beta-Pregnane-3,20-dione, 5beta-Dihydrotestosterone, benzil and retinal; when associated with F-176. Change in stereoselective activity by the reduction of 5beta-Pregnane-3,20-dione predominantly to the 3alpha-hydroxysteroid; when associated with F-176. Switch from 3beta-hydroxysteroid to 3alpha-hydroxysteroid oxidation; when associated with F-176. Loss of cold catalytic inactivation; when associated with F-176 and N-195. Increased reduction activity for renital and oxidation activity for retinol; when associated with F-176 and N-195.
- T195 (≠ I161) mutation to N: Loss of cold catalytic inactivation. Loss of cold catalytic inactivation; when associated with F-176 and L-179. Switch in stereoselective activity from 3beta-hydroxysteroid to 3alpha-hydroxysteroid oxidation; when associated with F-176 and L-179. Increased reduction activity for renital and oxidation activity for retinol; when associated with F-176 and L-179.
7v0hG Crystal structure of putative glucose 1-dehydrogenase from burkholderia cenocepacia in complex with NADP and a potential reaction product
31% identity, 99% coverage: 1:245/248 of query aligns to 6:253/253 of 7v0hG
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G18 (= G13), S20 (≠ N15), K21 (≠ S16), G22 (= G17), I23 (= I18), A43 (vs. gap), S44 (vs. gap), S45 (≠ T36), G68 (≠ A57), D69 (= D58), V70 (≠ Q59), N96 (= N85), S97 (≠ A86), G98 (= G87), Y100 (≠ V89), I144 (≠ L133), S146 (= S135), Y159 (= Y148), K163 (= K152), P189 (= P178), G190 (= G179), M191 (≠ P180), I192 (= I181), T194 (= T183), G196 (≠ I185), T197 (≠ M186)
- binding (2R)-2-(hydroxymethyl)pentanedioic acid: S146 (= S135), Y159 (= Y148), M191 (≠ P180), I202 (≠ L191)
6ci9D Rmm microcompartment-associated aminopropanol dehydrogenase NADP + aminoacetone holo-structure (see paper)
33% identity, 99% coverage: 1:246/248 of query aligns to 4:251/259 of 6ci9D
- active site: G20 (= G17), S145 (= S135), Y159 (= Y148)
- binding 1-aminopropan-2-one: F97 (≠ V89), S145 (= S135), T147 (= T137), W156 (vs. gap), Y159 (= Y148), G190 (= G179)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G16 (= G13), S18 (≠ N15), G20 (= G17), I21 (= I18), G40 (= G37), R41 (= R38), N42 (≠ R39), D66 (≠ L68), V67 (≠ A69), N93 (= N85), G95 (= G87), T143 (≠ L133), S145 (= S135), Y159 (= Y148), K163 (= K152), P189 (= P178), N191 (≠ P180), I192 (= I181), T194 (= T183), G196 (≠ I185), L197 (≠ M186)
5wuwA Serratia marcescens short-chain dehydrogenase/reductase f98l/f202l mutant (see paper)
34% identity, 98% coverage: 4:245/248 of query aligns to 3:244/245 of 5wuwA
- active site: G16 (= G17), S140 (= S134), Y154 (= Y148), L161 (= L155)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G13), R15 (≠ S16), I17 (= I18), Y36 (vs. gap), A37 (vs. gap), A38 (vs. gap), D63 (= D58), S64 (≠ Q59), N90 (= N85), A91 (= A86), G92 (= G87), Y154 (= Y148), K158 (= K152), G185 (= G179), P186 (= P180), V187 (≠ I181)
3pk0B Crystal structure of short-chain dehydrogenase/reductase sdr from mycobacterium smegmatis (see paper)
36% identity, 100% coverage: 1:247/248 of query aligns to 5:254/262 of 3pk0B
Query Sequence
>CA265_RS08650 CA265_RS08650 short-chain dehydrogenase
MSNLTGKKALVTGGNSGIGYATAKELKAQGAEVIITGRRKDAVDKAAAELGVIGLVADQS
QITAIEGLASDVESIFNKIDILFINAGVVEQSSIAEATEKSFDTIFGINFKGAYFTLSKF
IPLMNDGSSVVFLSSNTAHMDGAKSSIYTSSKSALNSVMRIAAVELAPRQIRVNSVSPGP
IATEIMNKMGLNEEQLNGINQWLISRSPLGKIGKSEDVAKMVAFFCGDAATYITGAEIVM
DGGMSLKA
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory