SitesBLAST
Comparing CA265_RS08865 FitnessBrowser__Pedo557:CA265_RS08865 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 11 hits to proteins with known functional sites (download)
6fahE Molecular basis of the flavin-based electron-bifurcating caffeyl-coa reductase reaction (see paper)
38% identity, 98% coverage: 3:317/322 of query aligns to 68:388/393 of 6fahE
- binding flavin-adenine dinucleotide: L180 (= L115), R200 (≠ K133), M281 (≠ R210), G282 (= G211), R307 (≠ K236), A308 (≠ P237), Q320 (≠ H249), V321 (= V250), G322 (= G251), Q323 (= Q252), T324 (= T253), G337 (= G266), I338 (= I267), S339 (= S268), Q343 (= Q272), H344 (= H273), N358 (= N287), K359 (= K288), L377 (≠ A306)
Sites not aligning to the query:
- binding iron/sulfur cluster: 7, 10, 13, 17, 18, 35, 36, 37, 38, 41, 45, 49
5ol2A The electron transferring flavoprotein/butyryl-coa dehydrogenase complex from clostridium difficile (see paper)
36% identity, 99% coverage: 2:321/322 of query aligns to 3:328/331 of 5ol2A
- binding calcium ion: E75 (≠ Q74), D188 (= D182)
- binding flavin-adenine dinucleotide: T117 (≠ A116), R136 (≠ K133), I147 (≠ T144), G216 (= G209), R217 (= R210), G218 (= G211), S242 (= S235), R243 (≠ K236), A244 (≠ P237), Q256 (≠ H249), V257 (= V250), G258 (= G251), T260 (= T253), G273 (= G266), I274 (= I267), S275 (= S268), A277 (= A270), Q279 (= Q272), H280 (= H273), N294 (= N287), K295 (= K288), D312 (= D305), V313 (≠ A306)
7qh2A Cryo-em structure of ldh-etfab complex from acetobacterium woodii (see paper)
32% identity, 98% coverage: 3:318/322 of query aligns to 13:332/337 of 7qh2A
- binding flavin-adenine dinucleotide: L125 (= L115), T126 (vs. gap), R144 (≠ K132), I155 (vs. gap), R224 (= R210), G225 (= G211), T249 (≠ S235), R250 (≠ K236), Q263 (≠ H249), I264 (≠ V250), G265 (= G251), L266 (≠ Q252), S267 (≠ T253), G280 (= G266), I281 (= I267), S282 (= S268), Q286 (= Q272), N301 (= N287), S302 (≠ K288), D303 (= D289), D319 (= D305), L320 (≠ A306)
2a1uA Crystal structure of the human etf e165betaa mutant (see paper)
34% identity, 98% coverage: 2:318/322 of query aligns to 3:313/315 of 2a1uA
- binding flavin-adenine dinucleotide: G204 (= G209), R205 (= R210), S230 (= S235), R231 (≠ K236), A232 (≠ P237), Q244 (≠ H249), V245 (= V250), G246 (= G251), T248 (= T253), G261 (= G266), I262 (= I267), S263 (= S268), A265 (= A270), Q267 (= Q272), H268 (= H273), N282 (= N287), K283 (= K288), D300 (= D305), L301 (≠ A306)
P13804 Electron transfer flavoprotein subunit alpha, mitochondrial; Alpha-ETF from Homo sapiens (Human) (see 6 papers)
34% identity, 98% coverage: 2:318/322 of query aligns to 21:331/333 of P13804
- G116 (= G100) to R: in GA2A; impaired protein stability and loss of electron transfer activity; dbSNP:rs119458971
- T171 (≠ N158) to I: decreased protein stability; dbSNP:rs1801591
- R223 (= R210) binding
- S248 (= S235) binding
- R249 (≠ K236) mutation to A: Loss of electron transfer activity.
- VGQT 263:266 (= VGQT 250:253) binding
- T266 (= T253) to M: in GA2A; decreased electron transfer activity; dbSNP:rs119458970
- SGAIQH 281:286 (= SGAIQH 268:273) binding
- N300 (= N287) binding
- DL 318:319 (≠ DA 305:306) binding
Sites not aligning to the query:
- 20:204 Domain I
- 205:333 Domain II
4kpuA Electron transferring flavoprotein of acidaminococcus fermentans: towards a mechanism of flavin-based electron bifurcation (see paper)
33% identity, 99% coverage: 3:321/322 of query aligns to 13:336/338 of 4kpuA
- binding flavin-adenine dinucleotide: L125 (= L115), R144 (≠ K133), I155 (≠ E145), G224 (= G209), R225 (= R210), G226 (= G211), S250 (= S235), R251 (≠ K236), A252 (≠ P237), Q264 (≠ H249), V265 (= V250), G266 (= G251), Q267 (= Q252), S268 (≠ T253), G281 (= G266), I282 (= I267), S283 (= S268), S285 (≠ A270), Q287 (= Q272), H288 (= H273), N302 (= N287), K303 (= K288), D320 (= D305), A321 (= A306)
1efpA Electron transfer flavoprotein (etf) from paracoccus denitrificans (see paper)
40% identity, 71% coverage: 84:313/322 of query aligns to 69:303/307 of 1efpA
- binding flavin-adenine dinucleotide: G199 (= G209), R200 (= R210), G201 (= G211), S225 (= S235), R226 (≠ K236), A227 (≠ P237), Q239 (≠ H249), V240 (= V250), G241 (= G251), T243 (= T253), G256 (= G266), I257 (= I267), S258 (= S268), A260 (= A270), Q262 (= Q272), H263 (= H273), N277 (= N287), K278 (= K288), D295 (= D305), L296 (≠ A306)
5ow0A Crystal structure of an electron transfer flavoprotein from geobacter metallireducens (see paper)
34% identity, 83% coverage: 53:318/322 of query aligns to 40:292/292 of 5ow0A
- binding flavin-adenine dinucleotide: G183 (= G209), R184 (= R210), G185 (= G211), S209 (= S235), R210 (≠ K236), Q223 (≠ H249), I224 (≠ V250), G225 (= G251), T227 (= T253), G240 (= G266), V241 (≠ I267), S242 (= S268), A244 (= A270), Q246 (= Q272), H247 (= H273), N261 (= N287), K262 (= K288), D279 (= D305), Y280 (≠ A306)
7koeB Electron bifurcating flavoprotein fix/etfabcx (see paper)
32% identity, 98% coverage: 3:318/322 of query aligns to 7:328/336 of 7koeB
- binding flavin-adenine dinucleotide: T121 (vs. gap), R140 (≠ K133), T142 (≠ A135), G219 (= G209), K220 (≠ R210), G221 (= G211), S245 (= S235), R246 (≠ K236), A247 (≠ P237), Q259 (≠ H249), V260 (= V250), G261 (= G251), Q262 (= Q252), T263 (= T253), G276 (= G266), S278 (= S268), Q282 (= Q272), H283 (= H273), N297 (= N287), I298 (≠ K288), L316 (≠ A306)
P53571 Electron transfer flavoprotein subunit alpha; Alpha-ETF; Electron transfer flavoprotein large subunit; ETFLS from Methylophilus methylotrophus (Bacterium W3A1) (see 2 papers)
27% identity, 98% coverage: 3:319/322 of query aligns to 4:321/321 of P53571
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
3clrD Crystal structure of the r236a etf mutant from m. Methylotrophus (see paper)
27% identity, 97% coverage: 3:313/322 of query aligns to 3:314/319 of 3clrD
- binding flavin-adenine dinucleotide: G209 (= G209), R210 (= R210), G211 (= G211), S235 (= S235), A236 (≠ K236), P237 (= P237), Q249 (≠ H249), V250 (= V250), G251 (= G251), Q252 (= Q252), S253 (≠ T253), G267 (= G266), I268 (= I267), S269 (= S268), S271 (≠ A270), Q273 (= Q272), H274 (= H273), N288 (= N287), T289 (≠ K288), D306 (= D305), I307 (≠ A306)
Query Sequence
>CA265_RS08865 FitnessBrowser__Pedo557:CA265_RS08865
MSVLVYVEQAEGKFKKSVFEAVSYAKAIADQQSTQLTAISIGDVADSELKELGKYGASKV
LNVSSDQLKTFVNQAYASVIAAAAEKEGADIVVLSNSFSGKGLAPRIAVKLKAGLADGAV
ELPSNDGKFSVKKTAFSGKAFAITELTSANKVIALNPNAFGVKENATDAAIEVFTADVKQ
SDLGTVIKEIVRATDKVSLPDAELVVSAGRGLKGPENWGMIEELAGLLGAATACSKPVSD
ADWRPHSEHVGQTGIAISPNLYIAIGISGAIQHLAGVSSSKVIVVINKDPEAPFFKVADY
GIVGDAFEVVPKLIEALKAHKG
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory