SitesBLAST
Comparing CA265_RS09010 FitnessBrowser__Pedo557:CA265_RS09010 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
6plgA Crystal structure of human phgdh complexed with compound 15 (see paper)
37% identity, 99% coverage: 2:314/315 of query aligns to 3:303/303 of 6plgA
6plfA Crystal structure of human phgdh complexed with compound 1 (see paper)
37% identity, 99% coverage: 2:314/315 of query aligns to 4:304/305 of 6plfA
7dkmA Phgdh covalently linked to oridonin (see paper)
37% identity, 99% coverage: 2:314/315 of query aligns to 4:304/306 of 7dkmA
- binding nicotinamide-adenine-dinucleotide: T74 (≠ V71), A102 (≠ V99), G148 (= G151), R151 (= R154), I152 (= I155), Y170 (= Y173), D171 (= D174), P172 (≠ L175), I173 (≠ Y176), H202 (= H214), T203 (= T215), P204 (= P216), T209 (≠ D219), C230 (= C240), A231 (≠ S241), R232 (= R242), H279 (= H289), G281 (= G291)
- binding (1beta,6beta,7beta,8alpha,9beta,10alpha,13alpha,14R,16beta)-1,6,7,14-tetrahydroxy-7,20-epoxykauran-15-one: C14 (≠ I12), K17 (≠ E15), I18 (≠ L16), E293 (≠ T303)
O43175 D-3-phosphoglycerate dehydrogenase; 3-PGDH; 2-oxoglutarate reductase; Malate dehydrogenase; EC 1.1.1.95; EC 1.1.1.399; EC 1.1.1.37 from Homo sapiens (Human) (see 3 papers)
37% identity, 99% coverage: 2:314/315 of query aligns to 8:308/533 of O43175
- T78 (≠ V71) binding
- R135 (≠ K134) to W: in PHGDHD; results in a 2-fold decrease in enzyme activity with 3-phosphohydroxypyruvate, but no change in substrate affinity; dbSNP:rs267606949
- RI 155:156 (= RI 154:155) binding
- D175 (= D174) binding
- T207 (= T215) binding
- CAR 234:236 (≠ CSR 240:242) binding
- D260 (= D266) binding
- V261 (= V267) to M: in PHGDHD; results in a four-fold decrease in substrate affinity and a slight increase in maximal enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs267606947
- HLGA 283:286 (≠ HIGA 289:292) binding
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 2 modified: N-acetylalanine
- 373 A → T: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs201553627
- 377 G → S: in PHGDHD; results in a 2-fold decrease in enzyme activity with 3-phosphohydroxypyruvate, but no change in substrate affinity; dbSNP:rs267606948
- 425 V → M: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs121907988
- 490 V → M: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs121907987
6rj5A Crystal structure of phgdh in complex with compound 39 (see paper)
37% identity, 98% coverage: 2:311/315 of query aligns to 3:300/301 of 6rj5A
7ewhA Crystal structure of human phgdh in complex with homoharringtonine (see paper)
37% identity, 98% coverage: 2:311/315 of query aligns to 3:300/302 of 7ewhA
- binding (3beta)-O~3~-[(2R)-2,6-dihydroxy-2-(2-methoxy-2-oxoethyl)-6-methylheptanoyl]cephalotaxine: L146 (≠ I150), G147 (= G151), L148 (≠ F152), G149 (= G153), R150 (= R154), I151 (= I155), G152 (= G156), D170 (= D174), H201 (= H214), T202 (= T215), P203 (= P216)
6rihA Crystal structure of phgdh in complex with compound 9 (see paper)
37% identity, 98% coverage: 2:311/315 of query aligns to 3:300/302 of 6rihA
6cwaA Crystal structure phgdh in complex with nadh and 3-phosphoglycerate at 1.77 a resolution (see paper)
37% identity, 98% coverage: 2:311/315 of query aligns to 2:299/299 of 6cwaA
- binding 1,4-dihydronicotinamide adenine dinucleotide: N96 (≠ S95), A100 (≠ V99), R149 (= R154), I150 (= I155), Y168 (= Y173), D169 (= D174), P170 (≠ L175), I171 (≠ Y176), H200 (= H214), T201 (= T215), P202 (= P216), T207 (≠ D219), C228 (= C240), A229 (≠ S241), R230 (= R242), H277 (= H289), G279 (= G291)
6rj3A Crystal structure of phgdh in complex with compound 15 (see paper)
38% identity, 97% coverage: 2:306/315 of query aligns to 2:294/297 of 6rj3A
1wwkA Crystal structure of phosphoglycerate dehydrogenase from pyrococcus horikoshii ot3
44% identity, 100% coverage: 1:314/315 of query aligns to 1:303/304 of 1wwkA
- active site: S96 (= S95), R230 (= R242), D254 (= D266), E259 (= E271), H278 (= H289)
- binding nicotinamide-adenine-dinucleotide: V100 (= V99), G146 (= G151), F147 (= F152), G148 (= G153), R149 (= R154), I150 (= I155), Y168 (= Y173), D169 (= D174), P170 (≠ L175), V201 (≠ T215), P202 (= P216), T207 (≠ P221), T228 (≠ C240), S229 (= S241), D254 (= D266), H278 (= H289), G280 (= G291)
6rj2A Crystal structure of phgdh in complex with compound 40 (see paper)
37% identity, 98% coverage: 3:311/315 of query aligns to 1:297/299 of 6rj2A
- binding ~{N}-[(1~{R})-1-[4-(ethanoylsulfamoyl)phenyl]ethyl]-2-methyl-5-phenyl-pyrazole-3-carboxamide: G146 (= G153), I148 (= I155), Y166 (= Y173), D167 (= D174), P168 (≠ L175), I169 (≠ Y176), I170 (≠ P177), H198 (= H214), T199 (= T215), L208 (≠ I222), R228 (= R242)
6plfB Crystal structure of human phgdh complexed with compound 1 (see paper)
37% identity, 98% coverage: 2:311/315 of query aligns to 2:291/292 of 6plfB
- binding 4-{(1S)-1-[(5-chloro-6-{[(5S)-2-oxo-1,3-oxazolidin-5-yl]methoxy}-1H-indole-2-carbonyl)amino]-2-hydroxyethyl}benzoic acid: R141 (= R154), Y160 (= Y173), D161 (= D174), P162 (≠ L175), I164 (≠ P177), L179 (= L201), T193 (= T215), P194 (= P216), S198 (≠ A218), L202 (≠ I222)
2eklA Structure of st1218 protein from sulfolobus tokodaii
39% identity, 96% coverage: 1:302/315 of query aligns to 5:295/312 of 2eklA
- active site: S100 (= S95), R232 (= R242), D256 (= D266), E261 (= E271), H282 (= H289)
- binding nicotinamide-adenine-dinucleotide: I76 (≠ V71), S100 (= S95), G148 (= G151), G150 (= G153), R151 (= R154), I152 (= I155), Y170 (= Y173), D171 (= D174), I172 (≠ L175), L173 (≠ Y176), H202 (= H214), V203 (≠ T215), T204 (≠ P216), I212 (= I222), T230 (≠ C240), S231 (= S241), D256 (= D266), G284 (= G291)
3dc2A Crystal structure of serine bound d-3-phosphoglycerate dehydrogenase from mycobacterium tuberculosis (see paper)
37% identity, 94% coverage: 10:306/315 of query aligns to 8:294/526 of 3dc2A
Sites not aligning to the query:
3ddnB Crystal structure of hydroxypyruvic acid phosphate bound d-3- phosphoglycerate dehydrogenase in mycobacterium tuberculosis (see paper)
37% identity, 94% coverage: 10:306/315 of query aligns to 9:295/525 of 3ddnB
P87228 Putative D-3-phosphoglycerate dehydrogenase; 3-PGDH; 2-oxoglutarate reductase; EC 1.1.1.95; EC 1.1.1.399 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
33% identity, 100% coverage: 1:315/315 of query aligns to 56:370/466 of P87228
- S87 (≠ P30) modified: Phosphoserine
- S258 (≠ A218) modified: Phosphoserine
5aovA Ternary crystal structure of pyrococcus furiosus glyoxylate hydroxypyruvate reductase in presence of glyoxylate (see paper)
33% identity, 91% coverage: 29:315/315 of query aligns to 34:313/334 of 5aovA
- active site: L100 (≠ S95), R241 (= R242), D265 (= D266), E270 (= E271), H288 (= H289)
- binding glyoxylic acid: M52 (≠ R47), L53 (≠ S48), L53 (≠ S48), Y74 (≠ G69), A75 (≠ G70), V76 (= V71), G77 (= G72), R241 (= R242), H288 (= H289)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V76 (= V71), T104 (≠ V99), F158 (= F152), G159 (= G153), R160 (= R154), I161 (= I155), S180 (≠ D174), R181 (≠ L175), A211 (≠ H214), V212 (≠ T215), P213 (= P216), T218 (≠ P221), I239 (≠ C240), A240 (≠ S241), R241 (= R242), H288 (= H289), G290 (= G291)
P0A9T0 D-3-phosphoglycerate dehydrogenase; PGDH; 2-oxoglutarate reductase; EC 1.1.1.95; EC 1.1.1.399 from Escherichia coli (strain K12) (see 2 papers)
34% identity, 99% coverage: 1:312/315 of query aligns to 11:315/410 of P0A9T0
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
1ybaA The active form of phosphoglycerate dehydrogenase (see paper)
34% identity, 99% coverage: 1:312/315 of query aligns to 7:311/406 of 1ybaA
- active site: N104 (≠ S95), R236 (= R242), D260 (= D266), E265 (= E271), H288 (= H289)
- binding 2-oxoglutaric acid: R56 (= R47), S57 (= S48), C79 (≠ G70), I80 (≠ V71)
- binding nicotinamide-adenine-dinucleotide: I80 (≠ V71), F102 (≠ A93), V108 (= V99), G154 (= G151), G156 (= G153), H157 (≠ R154), I158 (= I155), Y176 (= Y173), D177 (= D174), I178 (≠ L175), K181 (≠ E181), H206 (= H214), V207 (≠ T215), P208 (= P216), A234 (≠ C240), S235 (= S241), R236 (= R242), H288 (= H289), G290 (= G291)
- binding phosphate ion: G81 (= G72), N83 (≠ D74)
1psdA The allosteric ligand site in the vmax-type cooperative enzyme phosphoglycerate dehydrogenase (see paper)
34% identity, 99% coverage: 1:312/315 of query aligns to 5:309/404 of 1psdA
- active site: N102 (≠ S95), R234 (= R242), D258 (= D266), E263 (= E271), H286 (= H289)
- binding nicotinamide-adenine-dinucleotide: N102 (≠ S95), H155 (≠ R154), I156 (= I155), D175 (= D174), I176 (≠ L175), K179 (≠ E181), H204 (= H214), V205 (≠ T215), P206 (= P216), A232 (≠ C240), S233 (= S241), R234 (= R242), H286 (= H289)
Sites not aligning to the query:
Query Sequence
>CA265_RS09010 FitnessBrowser__Pedo557:CA265_RS09010
MKILANDGIDPIGKELLEKAGFQVDTETVPQDQLAEALKNYDAITVRSATKVRKELIDAV
PNIKLIGRGGVGMDNIDVEYARSQGINVVNTPAASSLSVAELVFSHLFTGIRFLQDANRK
MPVEGSTQFNNLKKAYAKGTELSGKTIGIIGFGRIGRATAKVALGLGMNVLAYDLYPSES
EITLEFQGGKSVSIPIKTVSLDEVITGSDFFSLHTPFADKPILGAEEFAKMKNGVGIVNC
SRGGTIDELALIDALNSGKVSFAGLDVFDNEPTPLAEILTHPKISLTPHIGASTNEAQER
IGTELATLIIEHFKK
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory