SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing CA265_RS10665 FitnessBrowser__Pedo557:CA265_RS10665 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 8 hits to proteins with known functional sites (download)

P48636 Cytokinin riboside 5'-monophosphate phosphoribohydrolase; AMP nucleosidase; PaLOG; EC 3.2.2.n1; EC 3.2.2.4 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
44% identity, 95% coverage: 4:190/197 of query aligns to 3:189/195 of P48636

query
sites
P48636

L

L

N

R

S

S

V

V

C

C

V

V

Y

F

C

C

G

G

S

A

N

S

F

P

N

G

G

A

D

S

I

P

T

V

L

Y

R

Q

K

E

A

A

I

A

K

V

Q

A

L

L

A

G

E

R

T

H

L

L

V

A

K

E

Q

R

Q

G

I

L

R

T

L

L

I

V

Y

Y

G

G

S

A

V

V

G

G

V

L

M

M

G

G

V

T

L

V

A

A

N

D

D

A

V

A

L

L

E

A

L

A

G

G

L

E

V

V

T

I

G

G

V

I

I

I

P

P

Q

Q

F

S

L

L

M

Q

D

E

K

A

E
|
E

V

I

G

G

H

H

Q

K

G

G

L

L

T

T

E

R

M

L

V

E

V

V

T

V

E

D

N

G

M
|
M

H

H

Q

A

R
|
R

K
|
K

Q

A

K

R

M

M

A

A

D

E

L

L

S

A

D

D

G

A

F

F

V

I

I

A

L

L

P

P

G

G

G
|
G

F
x
L

G
|
G

T
|
T

L
|
L

E
|
E

F

L

F

F

E
|
E

V

V

L

W

T

T

W

W

L

G

Q

Q

L

L

G

G

L

Y

H

H

N

A

K

K

P

P

I

L

G

G

V

L

L

L

N

E

V

V

S

N

G

G

F

F

Y

Y

D

D

P

P

L

L

F

L

A

T

Q

F

M

L

D

D

M

H

M

L

V

V

Q

D

S

E

K

R

F

F

L

V

K

R

P

A

A

E

N

H

R

R

D

G

L

M

V

L

F

Q

N

R

E

G

A

A

D

S

A

P

E

E

I

A

L

L

I

L

H

D

K

A

M

L

D

A

G

A

F

W

S

T

A

P

S

S

P

V

D

A

E

P

V

K

W

W

E74 (= E75) binding
M89 (= M90) mutation to A: Almost complete loss of activity.
R92 (= R93) mutation to A: Almost complete loss of activity.
RK 92:93 (= RK 93:94) binding
109:115 (vs. 110:116, 86% identical) binding
T112 (= T113) mutation to A: Almost complete loss of activity.
E114 (= E115) mutation to A: Almost complete loss of activity.
E115 (= E116) mutation to A: Almost complete loss of activity.
E118 (= E119) mutation to A: Retains 8% of wild-type activity.

5zbkA Crystal structure of type-i log from pseudomonas aeruginosa pao1 in complex with amp (see paper)
44% identity, 91% coverage: 4:183/197 of query aligns to 2:181/182 of 5zbkA

query
sites
5zbkA

L

L

N

R

S

S

V

V

C

C

V

V

Y

F

C

C

G

G

S
x
A

N

S

F

P

N

G

G

A

D

S

I

P

T

V

L

Y

R

Q

K

E

A

A

I

A

K

V

Q

A

L

L

A

G

E

R

T

H

L

L

V

A

K

E

Q

R

Q

G

I

L

R

T

L

L

I

V

Y

Y

G

G

S

A

V

V

G

G

V

L

M

M

G

G

V

T

L

V

A

A

N

D

D

A

V

A

L

L

E

A

L

A

G

G

L

E

V

V

T

I

G

G

V

I

I

I

P

P

Q

Q

F

S

L

L

M

Q

D

E

K

A

E
|
E

V

I

G

G

H

H

Q

K

G

G

L

L

T

T

E

R

M

L

V

E

V

V

T

V

E

D

N

G

M
|
M

H

H

Q

A

R
|
R

K
|
K

Q

A

K

R

M

M

A

A

D

E

L

L

S

A

D

D

G

A

F

F

V

I

I

A

L

L

P

P

G

G

G
|
G

F

L

G
|
G

T
|
T

L

L

E

E

E

A

F

L

F

F

E

E

V

V

L

W

T

T

W

W

L

G

Q

Q

L

L

G

G

L

Y

H

H

N

A

K

K

P

P

I

L

G

G

V

L

L

L

N

E

V

V

S

N

G

G

F

F

Y

Y

D

D

P

P

L

L

F

L

A

T

Q

F

M

L

D

D

M

H

M

L

V

V

Q

D

S

E

K

R

F

F

L

V

K

R

P

A

A

E

N

H

R

R

D

G

L

M

V

L

F

Q

N

R

E

G

A

A

D

S

A

P

E

E

I

A

L

L

I

L

H

D

K

A

M

L

D

A

G

A

F

W

S

T

binding adenosine monophosphate: A11 (≠ S13), E73 (= E75), M88 (= M90), R91 (= R93), K92 (= K94), G108 (= G110), G110 (= G112), T111 (= T113)

Q5ZC82 Cytokinin riboside 5'-monophosphate phosphoribohydrolase LOG; Protein LONELY GUY; EC 3.2.2.n1 from Oryza sativa subsp. japonica (Rice) (see paper)
42% identity, 92% coverage: 2:182/197 of query aligns to 33:213/242 of Q5ZC82

query
sites
Q5ZC82

N

S

K

R

L

F

N

R

S

R

V

I

C

C

V

V

Y

Y

C

C

G

G

S

S

N

A

F

K

N

G

G

R

D

K

I

A

T

S

L

Y

R

Q

K

D

A

A

I

A

K

V

Q

E

L

L

A

G

E

K

T

E

L

L

V

V

K

E

Q

R

Q

G

I

I

R

D

L

L

I

V

Y

Y

G
|
G

G

G

S

S

V

I

G

G

V

L

M

M

G

G

V

L

L

V

A

S

N

H

D

A

V

V

L

H

E

D

L

G

G

G

G

R

L

H

V

V

T

I

G

G

V

V

I

I

P

P

Q

K

F

S

L

L

M

M

D

P

K

R

E

E

V

V

G

T

H

G

Q

E

G

P

L

V

T

G

E

E

M

V

V

R

V

A

T

V

E

S

N

G

M

M

H

H

Q

E

R

R

K

K

Q

A

K

E

M

M

A

A

D

R

L

F

S

A

D

D

G

A

F

F

V

I

I

A

L

L

P

P

G

G

F

Y

G

G

T

T

L

L

E

E

F

L

E

E

V

V

L

I

T

T

W

W

L

A

Q

Q

L

L

G

G

L

I

H

H

N

K

K

K

P

P

I

V

G

G

V

L

L

L

N

N

V

V

S

D

G

G

F

F

Y

Y

D

D

P

P

L

F

F

L

A

S

Q

F

M

I

D

D

M

M

M

A

V

V

Q

S

S

E

K

G

F

F

L

I

K

A

P

E

A

D

N

A

R

R

D

R

L

I

V

I

F

I

N

S

E

A

A

P

D

T

A

A

E

R

I

E

L

L

I

V

H

L

K

K

M

L

D

E

G

E

F

Y

G73 (= G42) mutation to D: In LOG-4; decreased activity.

5zblD Crystal structure of type-i log from corynebacterium glutamicum in complex with amp (see paper)
43% identity, 91% coverage: 4:183/197 of query aligns to 2:180/181 of 5zblD

query
sites
5zblD

L

L

N

Q

S

R

V

V

C

T

V

V

Y

F

C

T

G

G

S
|
S

N

A

F

L

N

G

G

S

D

S

I

S

T

L

L

Y

R

T

K

Q

A

A

I

A

K

Q

Q

T

L

L

A

A

E

K

T

T

L

A

V

V

K

D

Q

R

Q

G

I

I

R

D

L

L

I

V

Y

Y

G

G

S

K

V

V

G

G

V

L

M
|
M

G

G

V

I

L

V

A

A

N

D

D

A

V

F

L

L

E

E

L

S

G

G

L

E

V

A

T

F

G

G

V

V

I

I

P

T

Q

E

F

S

L

L

M

M

D

-

K

K

E

G

V

L

G

G

H

H

Q

E

G

K

L

L

T

T

E

E

M

L

V

E

V

I

T

V

E

P

N

D

M

M

H

H

Q

I

R
|
R

K
|
K

Q

R

K

R

M

M

A

A

D

E

L

L

S

G

D

D

G

G

F

F

V

I

I

A

L

M

P

P

G

G

G
|
G

F

A

G
|
G

T
|
T

L

L

E
|
E

F

L

F

F

E

E

V

V

L

W

T

T

W

W

L

Q

Q

Q

L

L

G

G

L

I

H

H

N

Q

K

K

P

P

I

V

G

A

V

L

L

Y

N

D

V

V

S

D

G

G

F

F

Y

W

D

Q

P

P

L

L

F

L

A

E

Q

M

M

L

D

E

M

Q

M

M

V

T

Q

Q

S

R

K

G

F

F

L

I

K

K

P

R

A

D

N

F

R

F

D

E

L

C

V

L

F

I

N

V

E

E

A

S

D

D

A

P

E

H

I

A

L

L

I

L

H

K

K

A

M

M

D

Q

G

T

F

W

S

T

binding adenosine monophosphate: S11 (= S13), M47 (= M49), R90 (= R93), K91 (= K94), G107 (= G110), G109 (= G112), T110 (= T113), E112 (= E115), E113 (= E116)

5ajuA Crystal structure of ligand-free phosphoribohydroxylase lonely guy from claviceps purpurea in complex with phosphoribose (see paper)
35% identity, 91% coverage: 7:185/197 of query aligns to 5:206/217 of 5ajuA

query
sites
5ajuA

V

I

C

C

V

V

Y

F

C

C

G

G

S
|
S

N

S

F

G

N

G

G

A

D

S

I

P

T

A

L

H

R

M

K

E

A

A

I

A

K

R

Q

Q

L

L

A

G

E

R

T

V

L

M

V

A

K

E

Q

N

I

I

R

D

L

L

I

V

Y

Y

G

G

S

T

V

V

G

G

V

L

M

M

G

G

V

E

L

V

A

A

N

R

D

T

V

V

L

C

E

S

L

I

G

N

G

G

-

P

-

E

L

S

V

V

T

H

G

G

V

I

I

I

P

P

Q

E

F

A

L

L

M

V

-

R

-

Y

D

E

K

R

E

D

V

G

G

T

H

Y

Q

Q

G

T

L

V

-

K

-

D

-

N

-

K

-

Q

-

V

-

P

T

T

E

E

M

T

V

V

-

Y

-

G

-

R

-

T

V

V

T

V

E

K

N

D

M

M

H

H

Q

T

R
|
R

K

K

Q

K

K

M

M

M

A

A

D

E

-

E

-

V

-

I

-

S

-

G

L

P

S

G

D

S

G

G

F

F

V

I

I

G

L

L

P

S

G

G

G
|
G

F
x
Y

G
|
G

T
|
T

L

M

E

E

E
|
E

F

V

F

F

E

E

V

V

L

I

T

T

W

W

L

N

Q

Q

L

L

G

G

L

I

H

H

N

T

K

K

P

G

I

I

G

C

V

L

L

L

N

N

V

V

S

E

G

G

F

Y

Y

W

D

D

P

G

L

I

F

L

A

Q

Q

W

M

I

D

N

M

M

M

A

V

A

Q

A

S

Q

K

G

F

F

L

V

K

Q

P

P

A

G

N

N

R

E

D

T

L

I

V

V

F

V

N

S

E

A

A

G

D

D

A

A

E

E

I

G

L

A

I

V

H

R

K

A

M

L

D

R

G

E

F

Y

S

K

A

V

S

S

binding 5-O-phosphono-beta-D-ribofuranose: S11 (= S13), R108 (= R93), G131 (= G110), Y132 (≠ F111), G133 (= G112), T134 (= T113), E137 (= E116)

O05306 Cytokinin riboside 5'-monophosphate phosphoribohydrolase; Protein LONELY GUY homolog; LOG homolog; EC 3.2.2.n1 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
35% identity, 79% coverage: 6:160/197 of query aligns to 12:165/187 of O05306

query
sites
O05306

S

T

V

V

C

A

V

V

Y

Y

C

C

G

A

S

A

N

S

F

P

N

T

G

-

D

H

I

A

T

E

L

L

R

L

K

E

A

L

I

A

K

A

Q

E

L

V

A

G

E

A

T

A

L

I

V

A

K

G

Q

R

Q

G

I

W

R

T

L

L

I

V

Y

W

G

G

S

H

V

V

G

S

V

A

M
|
M

G

G

V

A

L

V

A

A

N

S

D

A

V

A

L

R

E

A

L

C

G

G

L

W

V

T

T

V

G

G

V

V

I

I

P

P

Q
x
K

F

M

L

L

M

V

D

Y

K

R

E
|
E

V

L

G

A

H

D

Q

H

G

D

L

A

T

D

E

E

M

L

V

I

V

V

T

T

E

D

N

T

M

M

H

W

Q

E

R
|
R

K

K

Q

Q

K

I

M

M

A

E

D

D

L

R

S

S

D

D

G

A

F

F

V

I

I

V

L

L

P

P

G

G

F

V

G

G

T
|
T

L

L

E
x
D

E
|
E

F

L

F

F

E

D

V

A

L

W

T

T

W

D

L

G

Q

Y

L

L

G

G

L

T

H

H

N

D

K

K

P

P

I

I

G

V

V

M

L

V

N

D

V

P

S

W

G

G

F

H

Y

F

D

D

P

G

L

L

F

R

A

A

Q

W

M

L

D

N

M

G

M

L

V

L

Q

D

S

T

K

G

F

Y

L

V

K

S

P

P

M54 (= M49) mutation to A: Large decrease in catalytic activity.
K74 (≠ Q69) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup); mutation to A: Abrogates pupylation. This mutant accumulates in M.tuberculosis with a functional proteasome system and sensitizes M.tuberculosis to NO. Decrease in catalytic activity.
E80 (= E75) mutation to A: Large decrease in catalytic activity.
R98 (= R93) mutation to A: Loss of catalytic activity.
T118 (= T113) mutation to A: Loss of catalytic activity.
DE 120:121 (≠ EE 115:116) mutation to AA: Loss of catalytic activity.

7w2iA Crystal structure of log (rv1205) from mycobacterium tuberculosis (see paper)
35% identity, 79% coverage: 6:160/197 of query aligns to 8:161/183 of 7w2iA

query
sites
7w2iA

S

T

V

V

C

A

V

V

Y

Y

C

C

G

A

S

A

N

S

F

P

N

T

G

-

D

H

I

A

T

E

L

L

R

L

K

E

A

L

I

A

K

A

Q

E

L

V

A

G

E

A

T

A

L

I

V

A

K

G

Q

R

Q

G

I

W

R

T

L

L

I

V

Y

W

G

G

S

H

V

V

G

S

V

A

M

M

G

G

V

A

L

V

A

A

N

S

D

A

V

A

L

R

E

A

L

C

G

G

L

W

V

T

T

V

G

G

V

V

I

I

P

P

Q

K

F

M

L

L

M

V

D

Y

K

R

E

E

V

L

G

A

H

D

Q

H

G

D

L

A

T

D

E

E

M

L

V

I

V

V

T

T

E

D

N

T

M

M

H

W

Q

E

R

R

K

K

Q

Q

K

I

M

M

A

E

D

D

L

R

S

S

D

D

G

A

F

F

V

I

I

V

L

L

P

P

G

G

F

V

G

G

T

T

L

L

E

D

E

E

F

L

F

F

E
x
D

V

A

L

W

T

T

W
x
D

L

G

Q

Y

L

L

G

G

L

T

H

H

N

D

K

K

P

P

I

I

G

V

V

M

L

V

N

D

V

P

S

W

G

G

F

H

Y

F

D

D

P

G

L

L

F

R

A

A

Q

W

M

L

D

N

M

G

M

L

V

L

Q

D

S

T

K

G

F

Y

L

V

K

S

P

P

binding magnesium ion: D120 (≠ E119), D124 (≠ W123)

3sbxA Crystal structure of a putative uncharacterized protein from mycobacterium marinum bound to adenosine 5'-monophosphate amp (see paper)
40% identity, 61% coverage: 39:159/197 of query aligns to 35:155/177 of 3sbxA

query
sites
3sbxA

L

L

I

V

Y

W

G

G

S

H

V

V

G

S

V

A

M

M

G

G

V

A

L

V

A

S

N

S

D

A

V

A

L

R

E

A

L

H

G

G

L

W

V

T

T

V

G

G

V

V

I

I

P

P

Q

K

F

M

L

L

M

V

D

H

K

R

E
|
E

V

L

G

A

H

D

Q

H

G

D

L

A

T

D

E

E

M

L

V

V

T

T

E

E

N

T

M
|
M

H

W

Q

E

R
|
R

K
|
K

Q

Q

K

V

M

M

A

E

D

D

L

R

S

A

D

N

G

A

F

F

V

I

I

T

L

L

P

P

G

G

G
|
G

F

V

G
|
G

T
|
T

L

L

E
x
D

E
|
E

F

L

E

D

V

V

L

W

T

T

W

E

L

G

Q

Y

L

L

G

G

L

M

H

H

N

D

K

K

P

S

I

I

G

V

V

V

L

L

N

D

V

P

S

W

G

G

F

H

Y

F

D

D

P

G

L

L

F

R

A

A

Q

W

M

L

D

S

M

E

M

L

V

A

Q

D

S

T

K

G

F

Y

L

V

K

S

binding adenosine monophosphate: E71 (= E75), M86 (= M90), R89 (= R93), K90 (= K94), G106 (= G110), G108 (= G112), T109 (= T113), D111 (≠ E115), E112 (= E116)

Query Sequence

>CA265_RS10665 FitnessBrowser__Pedo557:CA265_RS10665
MNKLNSVCVYCGSNFNGDITLRKAIKQLAETLVKQQIRLIYGGGSVGVMGVLANDVLELG
GLVTGVIPQFLMDKEVGHQGLTEMVVTENMHQRKQKMADLSDGFVILPGGFGTLEEFFEV
LTWLQLGLHNKPIGVLNVSGFYDPLFAQMDMMVQSKFLKPANRDLVFNEADAEILIHKMD
GFSASPDEVWFRERNLS

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory