SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing CA265_RS10815 CA265_RS10815 methylmalonyl-CoA epimerase to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 10 hits to proteins with known functional sites (download)

3oa4A Crystal structure of hypothetical protein bh1468 from bacillus halodurans c-125
40% identity, 96% coverage: 2:130/135 of query aligns to 3:131/138 of 3oa4A

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active site: H7 (= H6), I43 (≠ T42), E54 (= E53), H78 (= H77), E128 (= E127)
binding zinc ion: H7 (= H6), E54 (= E53), H78 (= H77), E128 (= E127)

6wf6A Streptomyces coelicolor methylmalonyl-coa epimerase (see paper)
34% identity, 95% coverage: 1:128/135 of query aligns to 2:135/141 of 6wf6A

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active site: H7 (= H6), E43 (≠ T42), Q60 (≠ E53), H84 (= H77), E134 (= E127)
binding cobalt (ii) ion: H7 (= H6), Q60 (≠ E53), H84 (= H77), E134 (= E127)

6xbqA Streptomyces coelicolor methylmalonyl-coa epimerase in complex with carboxy-carba(dethia)-coa
34% identity, 95% coverage: 1:128/135 of query aligns to 2:135/144 of 6xbqA

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active site: H7 (= H6), E43 (≠ T42), Q60 (≠ E53), H84 (= H77), E134 (= E127)
binding carboxymethyldethia coenzyme *a: Q39 (≠ E38), Q60 (≠ E53), A70 (= A63), K73 (= K66), W74 (≠ F67), H83 (= H76), H84 (= H77), L107 (= L100), F122 (= F115), P125 (= P118), K126 (= K119), L132 (= L125)
binding cobalt (ii) ion: H7 (= H6), Q60 (≠ E53), H84 (= H77), E134 (= E127)

6wfiA Methylmalonyl-coa epimerase in complex with 2-nitronate-propionyl-coa (see paper)
34% identity, 95% coverage: 1:128/135 of query aligns to 2:135/144 of 6wfiA

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active site: H7 (= H6), E43 (≠ T42), Q60 (≠ E53), H84 (= H77), E134 (= E127)
binding cobalt (ii) ion: H7 (= H6), Q60 (≠ E53), H84 (= H77), E134 (= E127)
binding [1-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethylsulfanyl]-1-oxidanylidene-propan-2-ylidene]-bis(oxidanidyl)azanium: H7 (= H6), Q39 (≠ E38), Q60 (≠ E53), A70 (= A63), K73 (= K66), W74 (≠ F67), H83 (= H76), H84 (= H77), L107 (= L100), G114 (= G107), S115 (≠ A108), I120 (≠ V113), F122 (= F115), P125 (= P118), L132 (= L125), E134 (= E127)

6wf7A Methylmalonyl-coa epimerase in complex with methylmalonyl-coa and nh4+ (see paper)
34% identity, 95% coverage: 1:128/135 of query aligns to 2:135/144 of 6wf7A

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active site: H7 (= H6), E43 (≠ T42), Q60 (≠ E53), H84 (= H77), E134 (= E127)
binding (S)-Methylmalonyl-Coenzyme A: Q39 (≠ E38), E43 (≠ T42), Q60 (≠ E53), A70 (= A63), W74 (≠ F67), H83 (= H76), H84 (= H77), L107 (= L100), G114 (= G107), S115 (≠ A108), F122 (= F115), P125 (= P118), K126 (= K119), L132 (= L125), E134 (= E127)
binding methylmalonyl-coenzyme a: Q39 (≠ E38), Q60 (≠ E53), A70 (= A63), W74 (≠ F67), H83 (= H76), H84 (= H77), L107 (= L100), G114 (= G107), S115 (≠ A108), I120 (≠ V113), F122 (= F115), P125 (= P118), K126 (= K119), L132 (= L125), E134 (= E127)

6wfhA Streptomyces coelicolor methylmalonyl-coa epimerase substrate complex (see paper)
34% identity, 95% coverage: 1:128/135 of query aligns to 2:135/139 of 6wfhA

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active site: H7 (= H6), E43 (≠ T42), Q60 (≠ E53), H84 (= H77), E134 (= E127)
binding cobalt (ii) ion: H7 (= H6), Q60 (≠ E53), H84 (= H77), E134 (= E127)
binding (3S,5R,9R,19E)-1-[(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-4-hydroxy-3-(phosphonooxy)tetrahydrofuran-2-yl]-3,5,9,19-tetrahydroxy-8,8,20-trimethyl-10,14-dioxo-2,4,6-trioxa-18-thia-11,15-diaza-3,5-diphosphahenicos-19-en-21-oic acid 3,5-dioxide (non-preferred name): H7 (= H6), Q39 (≠ E38), Q60 (≠ E53), A70 (= A63), K73 (= K66), W74 (≠ F67), H83 (= H76), H84 (= H77), L107 (= L100), G114 (= G107), S115 (≠ A108), F122 (= F115), P125 (= P118), K126 (= K119), L132 (= L125), E134 (= E127)

6xbtA Streptomyces coelicolor methylmalonyl-coa epimerase (q60a) in complex with 2-nitronate-propionyl-coa
34% identity, 95% coverage: 1:128/135 of query aligns to 2:135/140 of 6xbtA

query
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active site: H7 (= H6), E43 (≠ T42), A60 (≠ E53), H84 (= H77), E134 (= E127)
binding cobalt (ii) ion: H7 (= H6), H84 (= H77), E134 (= E127)
binding coenzyme a: Q39 (≠ E38), A70 (= A63), K73 (= K66), W74 (≠ F67), K77 (= K70), H83 (= H76), L107 (= L100), F122 (= F115), P125 (= P118), K126 (= K119), L132 (= L125)
binding [1-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethylsulfanyl]-1-oxidanylidene-propan-2-ylidene]-bis(oxidanidyl)azanium: H7 (= H6), Q39 (≠ E38), A70 (= A63), K73 (= K66), W74 (≠ F67), H83 (= H76), H84 (= H77), L107 (= L100), G114 (= G107), S115 (≠ A108), P125 (= P118), K126 (= K119), L132 (= L125), E134 (= E127)

Q96PE7 Methylmalonyl-CoA epimerase, mitochondrial; DL-methylmalonyl-CoA racemase; EC 5.1.99.1 from Homo sapiens (Human) (see paper)
38% identity, 96% coverage: 1:130/135 of query aligns to 45:175/176 of Q96PE7

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Q96PE7

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H50 (= H6) binding
R104 (≠ P60) to L: in dbSNP:rs6748672
H122 (= H77) binding
E172 (= E127) binding

6qh4C Crystal structure of human methylmalonyl-coa epimerase (mcee) p.Arg143cys variant
37% identity, 96% coverage: 1:130/135 of query aligns to 9:137/138 of 6qh4C

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6qh4C

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active site: H14 (= H6), V50 (≠ T42), E61 (= E53), H86 (= H77), E134 (= E127)
binding cobalt (ii) ion: H14 (= H6), H86 (= H77), E134 (= E127)

2qh0A Crystal structure of a glyoxalase from clostridium acetobutylicum
33% identity, 96% coverage: 3:131/135 of query aligns to 3:129/129 of 2qh0A

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2qh0A

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binding zinc ion: H6 (= H6), E53 (= E53), H76 (= H77), E125 (= E127)

Query Sequence

>CA265_RS10815 CA265_RS10815 methylmalonyl-CoA epimerase
MNKIEHIGIAVKDLNSSIAIYQKLLNTDCYKTEQVASEFVNTAFFKTGENKVELLQATAP
DSAIAKFIEKKGEGIHHIAFLVDDILAEMERLHQEGFVLLSESPKKGADDKMVCFVHPKD
TNGVLIEICQEIKWV

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory