SitesBLAST

1a56A Primary sequence and solution conformation of ferricytochromE C-552 from nitrosomonas europaea, nmr, mean structure refined with explicit hydrogen bond constraints (see paper)
43% identity, 8% coverage: 651:724/902 of query aligns to 7:80/81 of 1a56A

query
sites
1a56A

K

K

S

N

D
x
N

C
|
C

S

I

T

A

C
|
C

H
|
H

K

Q

E

V

A

E

A

T

V

K

S

V

I
x
V

G
|
G

P
|
P

A

A

F

L

N

K

K

D

I

I

A

A

K

K

Y

Y

K

A

N

D

D

K

S

D

K

D

A

A

V

A

D

T

Y

Y

L

L

A

A

S

G

K
|
K

V
x
I

I

K

A

G

G

G

S
|
S

H

S

G

G

V

V

W
|
W

G

G

E
x
Q

V
x
I

P

P

M
|
M

P

P

A

P

H
x
N

T
x
V

T
x
N

M

V

K

S

E

D

A

A

E

D

V

A

K

K

K

A

I

L

T

A

E

D

W

W

I

I

M

L

T

T

L

L

binding heme c: N9 (≠ D653), C10 (= C654), C13 (= C657), H14 (= H658), V21 (≠ I665), G22 (= G666), P23 (= P667), K45 (= K689), I46 (≠ V690), S50 (= S694), W54 (= W698), Q56 (≠ E700), I57 (≠ V701), M59 (= M703), N62 (≠ H706), V63 (≠ T707), N64 (≠ T708)

7cdyA Crystal structure of glucose dehydrogenase
25% identity, 31% coverage: 51:331/902 of query aligns to 3:238/346 of 7cdyA

query
sites
7cdyA

V

V

T

S

L

Q

A

L

Q

Q

G

D

E

G

F

L

T

E

E

H

P

P

T

W

E

S

M

L

T

A

I

F

L

L

P

P

-

A

N

E

L

Q

D

G

I

L

L

L

V

I

A

T

Q

E

R

R

R

P

G

G

E

R

V

L

L

R

I

L

Y

W

K

Q

N

-

Q

Q

T

D

K

K

K

G

I

L

K

S

E

P

A

P

G

-

K

-

L

-

D

-

V

-

Y

-

F

-

K

I

T

A

S

G

V

V

P

P

D

Q

V

V

N

Y

A

A

E

E

E

G

-

Q

-

G

G

G

L

L

G

E

M

V

A

L

A

P

D

A

P

P

K

D

F

F

A

A

V

A

N

S

Q

R

Y

R

I

V

Y

Y

L

L

-

S

F

F

Y

A

S

E

P

P

F

G

D

E

-

G

K

K

S

A

V

G

N

T

R

A

L

V

S

G

R

Y

F

G

K

R

L

L

I

S

N

D

D

D

Q

D

I

A

V

R

K

L

E

E

S

N

E

F

K

K

I

V

I

I

L

F

E

R

F

Q

Y

Q

S

P

Q

K

R

L

E

S

I

V

C

G

C

N

H

H

T

F

G

G

S

K

I

L

A

A

F

F

G

D

S

R

D

Q

N

G

L

Y

L

L

Y

F

V

I

S

A

T

L

G

G

D

E

N

N

S

-

T

-

P

-

F

-

D

-

V

-

P

-

K

-

Q

-

Y

-

V

-

N

-

K

-

G

-

Y

-

A

-

P

-

L

-

D

-

N

-

R

-

K

-

G

-

L

-

E

-

Q

-

Y

-

D

N

A

Q

R

R

R

P

S

T

A

A

G

Q

N

E

T

T

N

D

D

K

L

L

R

Q

G

G

K

K

I

L

L

V

R

R

I

L

K

T

V

A

N

E

E

G

D

-

G

-

T

-

Y

-

S

A

I

V

P

P

D

P

G

D

N

N

L

P

F

W

S

V

K

G

N

Q

D

A

P

G

K

K

A

-

R

R

P

P

E

E

I

V

Y

W

V

S

M

Y

G

G

N

H

R

R

N

N

P

P

Y

Q

R

G

I

L

S

A

V

L

D

N

Q

P

K

W

N

S

N

G

F

A

L

I

Y

W

W

E

G

H

E

E

V

-

G

-

P

-

D

-

A

-

S

-

N

-

D

-

A

-

L

-

R

H

G

G

P

P

R

R

G

G

Y

G

D

D

E
|
E

V

L

N

N

Q

I

A

P

R

L

K

P

A

G

G

K

N

N

F
x
Y

G

G

W

W

P

P

L

L

F

A

V

T

-

Y

G

G

N

I

N

N

Y

Y

A

S

binding calcium ion: E216 (= E310), Y226 (≠ F320)

3dasA Structure of the pqq-bound form of aldose sugar dehydrogenase (adh) from streptomyces coelicolor
28% identity, 32% coverage: 49:336/902 of query aligns to 8:240/334 of 3dasA

query
sites
3dasA

V

V

K

K

V

V

-

L

-

R

T

T

L

V

A

A

Q

T

G

G

E

-

F

L

T

N

E

S

P

P

T

W

E

G

M

L

T

A

I

P

L

L

P

P

N

G

L

G

D

D

I

L

L

L

V

V

A

S

Q

S

R

R

R

D

G

-

E

E

V

A

L

T

I

I

Y

T

K

R

-

V

-

D

N

A

Q

K

T

T

K

G

K

R

I

K

K

T

E

E

A

L

G

G

K

E

L

-

D

-

V

-

Y

-

F

-

K

-

T

-

S

-

V

V

P

P

D

G

V

V

N

S

-

P

-

S

A

G

E
|
E

E

G

G

G

L

L

G

G

M

I

A

A

A

L

D

S

P

P

K

D

F

Y

A

A

V

S

N

D

Q

H

Y

M

I

V

Y

Y

L

A

F

Y

Y

F

S

T

P

-

F

-

D

S

K

A

S

S

V

D

N

N

R

R

L

I

S

V

R

R

F

-

K

-

L

M

I

L

N

Y

D

D

Q

E

I

K

V

K

K

P

E

S

S

G

E

E

K

Q

I

L

I

G

L

A

E

P

F

D

Y

T

S

V

Q

F

R

R

E

G

I

I

-

P

-

K

-

G

C

V

C

I

H
|
H

T

N

G

G

S

R

I

I

A

A

F

F

G

G

S

P

D

D

N

K

L

M

L

L

Y

Y

V

A

S

G

T

T

G

G

D
x
E

N

S

S

G

T

D

P

-

F

-

D

-

V

-

P

-

K

-

Q

-

Y

-

V

-

N

-

K

-

G

-

Y

-

A

-

P

-

L

-

D

-

N

-

R

-

K

T

G

G

L

L

E

S

Q

Q

Y

-

D

-

A

-

R

-

S

-

A

-

G

-

N

D

T

R

N

K

D

S

L

L

R

G

G

G

K

K

I

I

L

L

R

R

I

M

K

-

V

-

N

-

E

-

D

-

G

-

T

-

Y

-

S

-

I

T

P

P

D

D

G

G

N

E

L

P

F

A

S

P

K

G

N

N

D

P

P

F

K

P

A

G

R

S

P

P

E

-

I

V

Y

Y

V

S

M

Y

G

G

N

H

R
|
R

N
|
N

P

V

Y
x
Q

R

G

I

L

S

A

V

W

D

D

Q

D

K
|
K

N

Q

N

R

F

-

L

L

Y

F

W

A

G

S

E

E

V
x
F

G
|
G

P
x
Q

D

D

A

T

S

-

N

-

D

-

A

-

L

-

R

-

G

-

P

-

R

-

G

-

Y

W

D

D

E
|
E

V

L

N

N

Q

A

A

I

R

K

K

P

A

G

G

D

N

N

F
x
Y

G

G

W

W

P

P

L

E

F

A

V
x
E

G

G

N
x
K

N

G

Y
x
G

A

G

Y

S

K

G

A

F

Y

H

D

D

active site: H127 (= H173), E146 (≠ D192), R189 (= R273), E230 (≠ V326), K232 (≠ N328), G234 (≠ Y330)
binding alpha-L-arabinopyranose: K199 (= K283)
binding calcium ion: G208 (= G293), Q209 (≠ P294), E214 (= E310), Y224 (≠ F320)
binding pyrroloquinoline quinone: E66 (= E111), H127 (= H173), R189 (= R273), N190 (= N274), Q192 (≠ Y276), F207 (≠ V292)

Sites not aligning to the query:

binding alpha-L-arabinopyranose: 248, 268, 269, 270, 278, 279
binding pyrroloquinoline quinone: 251, 268, 296, 298, 318

1corA Investigation of the solution conformation of cytochromE C-551 from pseudomonas stutzeri (see paper)
41% identity, 9% coverage: 645:723/902 of query aligns to 3:80/82 of 1corA

query
sites
1corA

G

G

K

E

T

A

I

L

M

F

L

K

K

S

S
x
K

D

P

C
|
C

S

A

T

A

C
|
C

H
|
H

K

S

E

I

A

D

A

A

V

K

S

L

I
x
V

G
|
G

P
|
P

A

A

F

F

N

K

K

E

I

V

A

A

K

K

Y

Y

K

A

N

G

D

Q

S

D

K

G

A

A

V

A

D

D

Y

L

L
|
L

A

A

S

G

K
x
H

V
x
I

I

K

A

N

G

G

S
|
S

H
x
Q

G
|
G

V
|
V

W
|
W

G
|
G

E

P

V
x
I

P

P

M
|
M

P

P

A

P

H
x
N

T

P

T

V

M

T

K

E

E

E

A

-

E

E

V

A

K

K

K

I

I

L

T

A

E

E

W

W

I

I

M

L

T

S

binding heme c: K10 (≠ S652), C12 (= C654), C15 (= C657), H16 (= H658), V23 (≠ I665), G24 (= G666), P25 (= P667), L44 (= L686), H47 (≠ K689), I48 (≠ V690), S52 (= S694), Q53 (≠ H695), G54 (= G696), V55 (= V697), W56 (= W698), G57 (= G699), I59 (≠ V701), M61 (= M703), N64 (≠ H706)

5xecA Heterodimer constructed from pa cyt c551-ht cyt c552 and ht cyt c552- pa cyt c551 chimeric proteins (see paper)
42% identity, 8% coverage: 654:724/902 of query aligns to 12:81/82 of 5xecA

query
sites
5xecA

C
|
C

S

V

T

A

C
|
C

H
|
H

K

A

E

I

A

D

A

T

V

K

S

K

I

V

G
|
G

P
|
P

A

A

F
x
Y

N

A

K

D

I

V

A

A

A

K

K

K

Y
|
Y

K

A

N

G

D

R

S

K

K

D

A

A

V

V

D

D

Y
|
Y

L

L

A

A

S

G

K

K

V
x
I

I

K

A

K

G

G

S

G

H
x
S

G
|
G

V
|
V

W
|
W

G
|
G

E

S

V
|
V

P

P

M
|
M

P

P

A

P

H
x
Q

T

N

T

V

M

T

K

-

E

D

A

A

E

E

V

A

K

K

K

Q

I

L

T

A

E

Q

W

W

I
|
I

M

L

T

S

L

I

binding heme c: C12 (= C654), C15 (= C657), H16 (= H658), G24 (= G666), P25 (= P667), Y27 (≠ F669), Y34 (= Y676), Y43 (= Y685), I48 (≠ V690), S53 (≠ H695), G54 (= G696), V55 (= V697), W56 (= W698), G57 (= G699), V59 (= V701), M61 (= M703), Q64 (≠ H706), I78 (= I721)

5aurA Hydrogenobacter thermophilus cytochrome c552 dimer formed by domain swapping at n-terminal region (see paper)
42% identity, 8% coverage: 654:724/902 of query aligns to 10:82/83 of 5aurA

query
sites
5aurA

C
|
C

S

M

T

A

C
|
C

H
|
H

K

D

E

L

A

K

A

A

-

G

V

K

S

K

I

V

G

G

P
|
P

A

A

F
x
Y

N

A

K

D

I

V

A

A

A

K

K

K

Y
|
Y

K

A

N

G

D

R

S

K

K

D

A

A

V

V

D

D

Y
|
Y

L

L

A

A

S

G

K

K

V
x
I

I

K

A

K

G

G

S

G

H
x
S

G
|
G

V
|
V

W
|
W

G
|
G

E

S

V
|
V

P
|
P

M
|
M

P

P

A

P

H
x
Q

T

N

T

V

M

T

K

-

E

D

A

A

E

E

V

A

K

K

K

Q

I

L

T

A

E

Q

W

W

I

I

M

L

T

S

L

I

binding heme c: C10 (= C654), C13 (= C657), H14 (= H658), P26 (= P667), Y28 (≠ F669), Y35 (= Y676), Y44 (= Y685), I49 (≠ V690), S54 (≠ H695), G55 (= G696), V56 (= V697), W57 (= W698), G58 (= G699), V60 (= V701), P61 (= P702), M62 (= M703), Q65 (≠ H706)

6kq1A Crystal structure of cytochrome c551 from pseudomonas sp. Strain mt-1.
36% identity, 9% coverage: 645:724/902 of query aligns to 3:81/82 of 6kq1A

query
sites
6kq1A

G

G

K
x
E

T

A

I

L

M

F

L

K

K

S

S
x
K

D

P

C
|
C

S

A

T

A

C
|
C

H
|
H

K

S

E

I

A

D

A

A

V

K

S

M

I

V

G
|
G

P
|
P

A

A

F

L

N

K

K

E

I
x
V

A

A

K

K

Y
|
Y

K

A

N

G

D

Q

S

E

K

G

A

A

V

A

D

D

Y

L

L
|
L

A

A

S

G

K
x
H

V
x
I

I

K

A

N

G

G

S

T

H
x
Q

G
|
G

V
x
N

W
|
W

G
|
G

E

P

V
x
I

P

P

M
|
M

P

P

A

P

H
x
N

T

P

T

V

M

T

K

E

E

E

A

-

E
|
E

V

A

K

K

K

T

I

L

T

A

E

E

W

W

I

V

M

L

T

S

L

L

binding heme c: C12 (= C654), C15 (= C657), H16 (= H658), G24 (= G666), P25 (= P667), V30 (≠ I672), Y34 (= Y676), L44 (= L686), H47 (≠ K689), I48 (≠ V690), Q53 (≠ H695), G54 (= G696), N55 (≠ V697), W56 (= W698), G57 (= G699), I59 (≠ V701), M61 (= M703), N64 (≠ H706)
binding zinc ion: E4 (≠ K646), K10 (≠ S652), E70 (= E713)

Sites not aligning to the query:

binding zinc ion: 2

7cgzA Glucose dehydrogenase
24% identity, 30% coverage: 51:321/902 of query aligns to 3:227/321 of 7cgzA

query
sites
7cgzA

V

V

T

S

L

Q

A

L

Q

Q

G

D

E

G

F

L

T

E

E

H

P

P

T

W

E

S

M

L

T

A

I

F

L

L

P

P

-

A

N

E

L

Q

D

G

I

L

L

L

V

I

A

T

Q

E

R

R

R

P

G

G

E

R

V

L

L

R

I

L

Y

W

K

Q

N

-

Q

Q

T

D

K

K

K

G

I

L

K

S

E

P

A

P

G

-

K

-

L

-

D

-

V

-

Y

-

F

-

K

I

T

A

S

G

V

V

P

P

D

Q

V

V

N

Y

A

A

E

E

E

G

-

Q

-

G

G

G

L

L

G

E

M

V

A

L

A

P

D

A

P

P

K

D

F

F

A

A

V

A

N

S

Q

R

Y

R

I

V

Y

Y

L

L

-

S

F

F

Y

A

S

E

P

P

F

G

D

E

-

G

K

K

S

A

V

G

N

T

R

A

L

V

S

G

R

Y

F

G

K

R

L

L

I

S

N

D

D

D

Q

D

I

A

V

R

K

L

E

E

S

N

E

F

K

K

I

V

I

I

L

F

E

R

F

Q

Y

Q

S

P

Q

K

R

L

E

S

I

V

C

G

C

N

H

H

T

F

G

G

S

K

I

L

A

A

F

F

G

D

S

R

D

Q

N

G

L

Y

L

L

Y

F

V

I

S

A

T

L

G

G

D

E

N

N

S

-

T

-

P

-

F

-

D

-

V

-

P

-

K

-

Q

-

Y

-

V

-

N

-

K

-

G

-

Y

-

A

-

P

-

L

-

D

-

N

-

R

-

K

-

G

-

L

-

E

-

Q

-

Y

-

D

N

A

Q

R

R

R

P

S

T

A

A

G

Q

N

E

T

T

N

D

D

K

L

L

R

Q

G

G

K

K

I

L

L

V

R

R

I

L

K

T

V

A

N

E

E

G

D

-

G

-

T

-

Y

-

S

A

I

V

P

P

D

P

G

D

N

N

L

P

F

W

S

V

K

G

N

Q

D

A

P

G

K

K

A

-

R

R

P

P

E

E

I

V

Y

W

V

S

M

Y

G

G

N

H

R

R

N

N

P

P

Y

Q

R

G

I

L

S

A

V

L

D

N

Q

P

K

W

N

S

N

G

F

A

L

I

Y

W

W

E

G

H

E

E

V

-

G

-

P

-

D

-

A

-

S

-

N

-

D

-

A

-

L

-

R

H

G

G

P

P

R

R

G

G

Y

G

D

D

E
|
E

V

L

N

N

Q

I

A

P

R

L

K

P

A

G

G

K

N

N

F
x
Y

G

G

binding calcium ion: E216 (= E310), Y226 (≠ F320)

2d0sA Crystal structure of the cytochrome c552 from moderate thermophilic bacterium, hydrogenophilus thermoluteolus (see paper)
41% identity, 8% coverage: 654:724/902 of query aligns to 10:78/79 of 2d0sA

query
sites
2d0sA

C
|
C

S

M

T

A

C
|
C

H
|
H

K

A

E

I

A

D

A

K

V

K

S

L

I

V

G

G

P
|
P

A

S

F

Y

N

K

K

D

I

V

A

A

A

K

K

K

Y
|
Y

K

T

N

E

D

A

S

D

K

-

A

-

V

V

D

P

Y

K

L

L

A

V

S

E

K
|
K

V
|
V

I

K

A

K

G

G

S

G

H
x
A

G
|
G

V
|
V

W
|
W

G
|
G

E

P

V
|
V

P

P

M
|
M

P
|
P

A

P

H
|
H

T

P

T

Q

M

V

K

A

E

E

A

A

E

D

V

I

K

E

K

K

I

I

T

V

E

R

W

W

I

V

M

L

T

T

L

L

binding heme c: C10 (= C654), C13 (= C657), H14 (= H658), P23 (= P667), Y32 (= Y676), K43 (= K689), V44 (= V690), A49 (≠ H695), G50 (= G696), V51 (= V697), W52 (= W698), G53 (= G699), V55 (= V701), M57 (= M703), P58 (= P704), H60 (= H706)

1cchA The solution conformation of cytochromE C-551 from p.Stutzeri zobell determined by nmr+ (see paper)
36% identity, 9% coverage: 645:724/902 of query aligns to 3:81/82 of 1cchA

query
sites
1cchA

G

G

K

E

T

A

I

L

M

F

L

K

K

S

S

K

D

P

C
|
C

S

A

T

A

C
|
C

H
|
H

K

S

E

V

A

D

A

T

V

K

S

M

I

V

G

G

P

P

A

A

F

L

N

K

K

E

I

V

A

A

K

K

Y

N

K

A

N

G

D

V

S

E

K

G

A

A

V

A

D

D

Y

T

L
|
L

A

A

S

L

K
x
H

V
x
I

I

K

A

N

G

G

S
|
S

H
x
Q

G

G

V
|
V

W
|
W

G
|
G

E

P

V
x
I

P

P

M
|
M

P
|
P

A

P

H
x
N

T

P

T

V

M

T

K

E

E

E

A

-

E

E

V

A

K

K

K

I

I

L

T

A

E

E

W

W

I

V

M

L

T

S

L

L

binding heme c: C12 (= C654), C15 (= C657), H16 (= H658), L44 (= L686), H47 (≠ K689), I48 (≠ V690), S52 (= S694), Q53 (≠ H695), V55 (= V697), W56 (= W698), G57 (= G699), I59 (≠ V701), M61 (= M703), P62 (= P704), N64 (≠ H706)

1cq1A Soluble quinoprotein glucose dehydrogenase from acinetobacter calcoaceticus in complex with pqqh2 and glucose (see paper)
25% identity, 32% coverage: 35:327/902 of query aligns to 9:264/444 of 1cq1A

query
sites
1cq1A

F

F

T

A

K

K

S

A

K

K

I

S

D

E

T

N

P

F

D

D

Q

K

D

-

R

-

F

-

V

-

K

K

V

V

T

I

L

L

A

S

Q

-

G

-

E

N

F

L

T

N

E

K

P

P

T

H

E

A

M

L

T

L

I

W

L

G

P

P

N

D

L

N

D

Q

I

I

L

W

V

L

A

T

Q

E

R

R

R

A

G

T

E

G

V

K

L

I

I

L

Y

R

K

V

N

N

Q

-

T

-

K

-

I

-

K

P

E

E

A

S

G

G

K

S

L

V

D

K

V

T

Y

V

F

F

K

Q

T

V

S

P

-

E

-

I

V

V

P

N

D

D

V

A

N

D

A

G

E
x
Q

E

N

G

G

L

L

G

G

M

F

A

A

A

F

D

H

P

P

K

D

F

F

A

K

V

N

N

N

Q

P

Y

Y

I

I

Y

Y

L

I

-

S

-

G

-

T

F

F

Y

K

S

N

P

P

F

K

-

P

D

N

K

Q

S

T

V

I

N

I

R

R

L

R

S

Y

R

T

F

Y

K

N

L

K

I

S

N

T

D

D

Q

T

I

L

V

E

K

K

E

P

S

V

E

D

K

-

I

L

L

A

E

G

F

L

Y

P

S

S

Q

S

R

K

E
x
D

I

-

C

-

H
|
H

T

Q

G

S

G

G

S

R

I

L

A

V

F

I

G

G

S

P

D

D

N

Q

L

K

L

I

Y

Y

V

Y

S

T

T

I

G

G

D
|
D

N

-

S

-

T

-

P

-

F

-

D

-

V

-

P

-

K

-

Q

Q

Q

G

Y

R

V

N

N
x
Q

K

L

G

A

Y

Y

A

L

P

F

L

L

D

P

N

N

R

Q

K

A

G

Q

L

H

E

T

Q

P

Y

T

D

Q

A

Q

R

E

R

L

S

N

A

G

G

K

N

D

T

Y

N

H

D

T

L

Y

R

M

G

G

K

K

I

V

L

L

R

R

I

L

K

-

V

-

N

N

E

L

D

D

G

G

T

-

Y

-

S

S

I

I

P

P

D

-

G

-

N

-

L

-

F

-

S

-

K

K

N

D

D

N

P

P

K

S

-

F

-

N

-

G

A

V

R

V

P

S

E

H

I

I

Y

Y

V

T

M

L

G

G

N

H

R
|
R

N
|
N

P

P

Y

Q

R

G

I

L

S

A

V

F

D

T

Q

P

K

N

N

G

N

K

F

L

L

L

Y

-

W

-

G

-

E

-

V

-

G

-

P

-

D

-

A

-

S

-

N

-

D

-

D

Q

A

S

L

E

R
x
Q

G
|
G

P
|
P

R

N

G

S

Y

D

D

D

E
|
E

V

I

N

N

Q

L

A

I

R

V

K

K

A

G

G

G

N

N

F
x
Y

G

G

W

W

P

P

L

N

F

V

V
x
A

G

G

active site: H138 (= H173), D157 (= D192), R222 (= R273), A263 (≠ V326)
binding beta-D-glucopyranose: D137 (≠ E169), H138 (= H173), Q162 (≠ N206)
binding calcium ion: G241 (= G304), P242 (= P305), E247 (= E310), Y257 (≠ F320), A263 (≠ V326)
binding pyrroloquinoline quinone: Q76 (≠ E111), H138 (= H173), R222 (= R273), N223 (= N274), Q240 (≠ R303)

Sites not aligning to the query:

active site: 265, 267, 303
binding beta-D-glucopyranose: 337
binding calcium ion: 265, 267, 303
binding pyrroloquinoline quinone: 337, 342, 344, 370, 371, 400, 402

1c9uA Crystal structure of the soluble quinoprotein glucose dehydrogenase in complex with pqq (see paper)
25% identity, 32% coverage: 35:327/902 of query aligns to 9:264/444 of 1c9uA

query
sites
1c9uA

F

F

T

A

K

K

S

A

K

K

I

S

D

E

T

N

P

F

D

D

Q

K

D

-

R

-

F

-

V

-

K

K

V

V

T

I

L

L

A

S

Q

-

G

-

E

N

F

L

T

N

E

K

P

P

T

H

E

A

M

L

T

L

I

W

L

G

P

P

N

D

L

N

D

Q

I

I

L

W

V

L

A

T

Q

E

R

R

R

A

G

T

E

G

V

K

L

I

I

L

Y

R

K

V

N

N

Q

-

T

-

K

-

I

-

K

P

E

E

A

S

G

G

K

S

L

V

D

K

V

T

Y

V

F

F

K

Q

T

V

S

P

-

E

-

I

V

V

P

N

D

D

V

A

N

D

A

G

E
x
Q

E

N

G

G

L

L

G

G

M

F

A

A

A

F

D

H

P

P

K

D

F

F

A

K

V

N

N

N

Q

P

Y

Y

I

I

Y

Y

L

I

-

S

-

G

-

T

F

F

Y

K

S

N

P

P

F

K

-

P

D

N

K

Q

S

T

V

I

N

I

R

R

L

R

S

Y

R

T

F

Y

K

N

L

K

I

S

N

T

D

D

Q

T

I

L

V

E

K

K

E

P

S

V

E

D

K

-

I

L

L

A

E

G

F

L

Y

P

S

S

Q

S

R

K

E

D

I

-

C

-

H
|
H

T

Q

G

S

G

G

S

R

I

L

A

V

F

I

G

G

S

P

D

D

N

Q

L

K

L

I

Y

Y

V

Y

S

T

T

I

G

G

D
|
D

N

-

S

-

T

-

P

-

F

-

D

-

V

-

P

-

K

-

Q

Q

Q

G

Y

R

V

N

N

Q

K

L

G

A

Y

Y

A

L

P

F

L

L

D

P

N

N

R

Q

K

A

G

Q

L

H

E

T

Q

P

Y

T

D

Q

A

Q

R

E

R

L

S

N

A

G

G

K

N

D

T

Y

N

H

D

T

L

Y

R

M

G

G

K

K

I

V

L

L

R

R

I

L

K

-

V

-

N

N

E

L

D

D

G

G

T

-

Y

-

S

S

I

I

P

P

D

-

G

-

N

-

L

-

F

-

S

-

K

K

N

D

D

N

P

P

K

S

-

F

-

N

-

G

A

V

R

V

P

S

E

H

I

I

Y

Y

V

T

M

L

G

G

N

H

R
|
R

N
|
N

P

P

Y

Q

R

G

I

L

S

A

V

F

D

T

Q

P

K

N

N

G

N

K

F

L

L

L

Y

-

W

-

G

-

E

-

V

-

G

-

P

-

D

-

A

-

S

-

N

-

D

-

D

Q

A

S

L

E

R
x
Q

G
|
G

P
|
P

R

N

G

S

Y

D

D

D

E
|
E

V

I

N

N

Q

L

A

I

R

V

K

K

A

G

G

G

N

N

F
x
Y

G

G

W

W

P

P

L

N

F

V

V
x
A

G

G

active site: H138 (= H173), D157 (= D192), R222 (= R273), A263 (≠ V326)
binding calcium ion: R222 (= R273), G241 (= G304), P242 (= P305), E247 (= E310), Y257 (≠ F320), A263 (≠ V326)
binding pyrroloquinoline quinone: Q76 (≠ E111), H138 (= H173), R222 (= R273), N223 (= N274), Q240 (≠ R303)

Sites not aligning to the query:

active site: 265, 267, 303
binding calcium ion: 265, 267, 303
binding pyrroloquinoline quinone: 337, 340, 342, 370, 371, 400, 402

1cruA Soluble quinoprotein glucose dehydrogenase from acinetobacter calcoaceticus in complex with pqq and methylhydrazine (see paper)
25% identity, 32% coverage: 35:327/902 of query aligns to 9:268/448 of 1cruA

query
sites
1cruA

F

F

T

A

K

K

S

A

K

K

I

S

D

E

T

N

P

F

D

D

Q

K

D

-

R

-

F

-

V

-

K

K

V

V

T

I

L

L

A

S

Q

N

G

-

E

-

F

L

T

N

E

K

P

P

T

H

E

A

M

L

T

L

I

W

L

G

P

P

N

D

L

N

D

Q

I

I

L

W

V

L

A

T

Q

E

R

R

R

A

G

T

E

G

V

K

L

I

I

L

Y

R

K

V

N

N

Q

-

T

-

K

-

I

-

K

P

E

E

A

S

G

G

K

S

L

V

D

K

V

T

Y

V

F

F

K

Q

T

V

S

P

-

E

-

I

V

V

P

N

D

D

V

A

N

D

A

G

E
x
Q

E

N

G

G

L

L

G

G

M

F

A

A

A

F

D

H

P

P

K

D

F

F

A

K

V

N

N

N

Q

P

Y

Y

I

I

Y

Y

L

I

-

S

-

G

-

T

F

F

Y

K

S

N

P

P

F

K

D

S

K

K

S

E

V

L

-

P

N

N

R

Q

L

T

S

I

R

I

F

R

K

R

L

Y

I

T

N

Y

D

N

Q

K

I

S

V

T

K

D

E

T

S

L

E

E

K

K

I

P

I

V

L

D

E

L

F

L

Y

A

S

G

Q

L

R

P

E

S

I

S

C

K

C

D

H
|
H

T

Q

G

S

G

G

S

R

I

L

A

V

F

I

G

G

S

P

D

D

N

Q

L

K

L

I

Y

Y

V

Y

S

T

T

I

G

G

D
|
D

N

-

S

-

T

-

P

-

F

-

D

-

V

-

P

-

K

-

Q

Q

Q

G

Y

R

V

N

N

Q

K

L

G

A

Y

Y

A

L

P

F

L

L

D

P

N

N

R

Q

K

A

G

Q

L

H

E

T

Q

P

Y

T

D

Q

A

Q

R

E

R

L

S

N

A

G

G

K

N

D

T

Y

N

H

D

T

L

Y

R

M

G

G

K

K

I

V

L

L

R

R

I

L

K

-

V

-

N

N

E

L

D

D

G

G

T

-

Y

-

S

S

I

I

P

P

D

-

G

-

N

-

L

-

F

-

S

-

K

K

N

D

D

N

P

P

K

S

-

F

-

N

-

G

A

V

R

V

P

S

E

H

I

I

Y

Y

V

T

M

L

G

G

N

H

R
|
R

N
|
N

P

P

Y

Q

R

G

I

L

S

A

V

F

D

T

Q

P

K

N

N

G

N

K

F

L

L

L

Y

-

W

-

G

-

E

-

V

-

G

-

P

-

D

-

A

-

S

-

N

-

D

-

D

Q

A

S

L

E

R
x
Q

G
|
G

P
|
P

R

N

G

S

Y

D

D

D

E
|
E

V

I

N

N

Q

L

A

I

R

V

K

K

A

G

G

G

N

N

F
x
Y

G

G

W

W

P

P

L

N

F

V

V
x
A

G

G

active site: H142 (= H173), D161 (= D192), R226 (= R273), A267 (≠ V326)
binding calcium ion: G245 (= G304), P246 (= P305), E251 (= E310), Y261 (≠ F320), A267 (≠ V326)
binding pyrroloquinoline quinone: Q76 (≠ E111), H142 (= H173), R226 (= R273), N227 (= N274), Q244 (≠ R303)

Sites not aligning to the query:

active site: 269, 271, 307
binding calcium ion: 269, 271, 307
binding methylhydrazine: 341, 344
binding pyrroloquinoline quinone: 341, 346, 348, 374, 375, 404, 406

P13650 Quinoprotein glucose dehydrogenase B; Glucose dehydrogenase B [pyrroloquinoline-quinone]; Soluble glucose dehydrogenase; s-GDH; EC 1.1.5.2 from Acinetobacter calcoaceticus (see paper)
26% identity, 35% coverage: 8:327/902 of query aligns to 11:294/478 of P13650

query
sites
P13650

L

L

S

S

A

A

L

V

A

Q

F

L

V

V

F

T

I

L

S

S

A

A

T

F

T

A

L

-

N

D

K

V

N

P

I

L

Y

T

E

P

S

S

K

Q

A

-

F

-

E

-

F

-

F

F

T

A

K

K

S

A

K

K

I

S

D

E

T

N

P

F

D

D

Q

K

D

-

R

-

F

-

V

-

K

K

V

V

T

I

L

L

A

S

Q

-

G

-

E

N

F

L

T

N

E

K

P

P

T

H

E

A

M

L

T

L

I

W

L

G

P

P

N

D

L

N

D

Q

I

I

L

W

V

L

A

T

Q

E

R

R

R

A

G

T

E

G

V

K

L

I

I

L

Y

R

K

V

N

N

Q

-

T

-

K

-

I

-

K

P

E

E

A

S

G

G

K

S

L

V

D

K

V

T

Y

V

F

F

K

Q

T

V

S

P

-

E

-

I

V

V

P

N

D

D

V

A

N

D

A

G

E
x
Q

E

N

G

G

L

L

G

G

M

F

A

A

A

F

D

H

P

P

K

D

F

F

A

K

V

N

N

N

Q

P

Y

Y

I

I

Y

Y

L

I

-

S

-

G

-

T

F

F

Y

K

S

N

P

P

F

-

D

-

K

K

S

S

V

T

N

D

R

K

L

-

S

-

R

-

F

-

K

E

L

L

I

P

N

N

D

Q

Q

T

I

I

V

I

K

R

E

R

S

Y

-

T

-

Y

-

N

-

K

-

S

-

T

-

D

-

T

-

L

E

E

K

K

I

P

I

V

L

D

E

L

F

L

Y

A

S

G

Q

L

R

P

E

S

I

S

C

K

C
x
D

H

H

T

Q

G

S

G

G

S

R

I

L

A

V

F

I

G

G

S

P

D

D

N

Q

L

K

L

I

Y

Y

V

Y

S

T

T

I

G

G

D

D

N

-

S

-

T

-

P

-

F

-

D

-

V

-

P

-

K

-

Q

Q

Q

G

Y

R

V

N

N
x
Q

K

L

G

A

Y

Y

A

L

P

F

L

L

D

P

N

N

R

Q

K

A

G

Q

L

H

E

T

Q

P

Y

T

D

Q

A

Q

R

E

R

L

S

N

A

G

G

K

N

D

T

Y

N

H

D

T

L

Y

R

M

G

G

K

K

I

V

L

L

R

R

I

L

K

-

V

-

N

N

E

L

D

D

G

G

T

-

Y

-

S

S

I

I

P

P

D

-

G

-

N

-

L

-

F

-

S

-

K

K

N

D

D

N

P

P

K

S

-

F

-

N

-

G

A

V

R

V

P

S

E

H

I

I

Y

Y

V

T

M

L

G

G

N

H

R
|
R

N

N

P

P

Y

Q

R

G

I

L

S

A

V

F

D

T

Q

P

K

N

N

G

N

K

F

L

L

L

Y

-

W

-

G

-

E

-

V

-

G

-

P

-

D

-

A

-

S

-

N

-

D

-

D

Q

A

S

L

E

R

Q

G
|
G

P
|
P

R

N

G

S

Y

D

D

D

E
|
E

V

I

N

N

Q

L

A

I

R

V

K

K

A

G

G

G

N

N

F
x
Y

G

G

W

W

P

P

L

N

F

V

V
x
A

G

G

Q100 (≠ E111) binding
D167 (≠ C172) binding
Q192 (≠ N206) binding
R252 (= R273) binding
G271 (= G304) binding
P272 (= P305) binding
E277 (= E310) binding
Y287 (≠ F320) binding
A293 (≠ V326) binding

Sites not aligning to the query:

295 binding
297 binding
333 binding
367 binding
372 binding
401 binding

5minB Apo form of the soluble pqq-dependent glucose dehydrogenase from acinetobacter calcoaceticus
25% identity, 32% coverage: 35:327/902 of query aligns to 9:270/453 of 5minB

query
sites
5minB

F

F

T

A

K

K

S

A

K

K

I

S

D

E

T

N

P

F

D

D

Q

K

D

-

R

-

F

-

V

-

K

K

V

V

T

I

L

L

A

S

Q

-

G

-

E

N

F

L

T

N

E

K

P

P

T

H

E

A

M

L

T

L

I

W

L

G

P

P

N

D

L

N

D

Q

I

I

L

W

V

L

A

T

Q

E

R

R

R

A

G

T

E

G

V

K

L

I

I

L

Y

R

K

V

N

N

Q

-

T

-

K

-

I

-

K

P

E

E

A

S

G

G

K

S

L

V

D

K

V

T

Y

V

F

F

K

Q

T

V

S

P

-

E

-

I

V

V

P

N

D

D

V

A

N

D

A

G

E

Q

E

N

G

G

L

L

G

G

M

F

A

A

A

F

D

H

P

P

K

D

F

F

A

K

V

N

N

N

Q

P

Y

Y

I

I

Y

Y

L

I

-

S

-

G

-

T

F

F

Y

K

S

N

P

P

F

K

-

S

-

T

-

D

-

K

-

A

-

L

-

P

D

N

K

Q

S

T

V

I

N

I

R

R

L

R

S

Y

R

T

F

Y

K

N

L

K

I

S

N

T

D

D

Q

T

I

L

V

E

K

K

E

P

S

V

E

D

K

-

I

L

L

A

E

G

F

L

Y

P

S

S

Q

S

R

K

E

D

I

-

C

-

H
|
H

T

Q

G

S

G

G

S

R

I

L

A

V

F

I

G

G

S

P

D

D

N

Q

L

K

L

I

Y

Y

V

Y

S

T

T

I

G

G

D
|
D

N

-

S

-

T

-

P

-

F

-

D

-

V

-

P

-

K

-

Q

Q

Q

G

Y

R

V

N

N

Q

K

L

G

A

Y

Y

A

L

P

F

L

L

D

P

N

N

R

Q

K

A

G

Q

L

H

E

T

Q

P

Y

T

D

Q

A

Q

R

E

R

L

S

N

A

G

G

K

N

D

T

Y

N

H

D

T

L

Y

R

M

G

G

K

K

I

V

L

L

R

R

I

L

K

-

V

-

N

N

E

L

D

D

G

G

T

-

Y

-

S

S

I

I

P

P

D

-

G

-

N

-

L

-

F

-

S

-

K

K

N

D

D

N

P

P

K

S

-

F

-

N

-

G

A

V

R

V

P

S

E

H

I

I

Y

Y

V

T

M

L

G

G

N

H

R
|
R

N

N

P

P

Y

Q

R

G

I

L

S

A

V

F

D

T

Q

P

K

N

N

G

N

K

F

L

L

L

Y

-

W

-

G

-

E

-

V

-

G

-

P

-

D

-

A

-

S

-

N

-

D

-

D

Q

A

S

L

E

R

Q

G

G

P

P

R

N

G

S

Y

D

D

D

E
|
E

V

I

N

N

Q

L

A

I

R

V

K

K

A

G

G

G

N

N

F
x
Y

G

G

W

W

P

P

L

N

F

V

V
x
A

G

G

active site: H144 (= H173), D163 (= D192), R228 (= R273), A269 (≠ V326)
binding calcium ion: E253 (= E310), Y263 (≠ F320), A269 (≠ V326)

Sites not aligning to the query:

active site: 271, 273, 309
binding calcium ion: 271, 273, 309

Query Sequence

>CA265_RS11590 FitnessBrowser__Pedo557:CA265_RS11590
MKTTFTLLLSALAFVFISATTLNKNIYESKAFEFFTKSKIDTPDQDRFVKVTLAQGEFTE
PTEMTILPNLDILVAQRRGEVLIYKNQTKKIKEAGKLDVYFKTSVPDVNAEEGLLGMAAD
PKFAVNQYIYLFYSPFDKSVNRLSRFKLINDQIVKESEKIILEFYSQREICCHTGGSIAF
GSDNLLYVSTGDNSTPFDVPKQQYVNKGYAPLDNRKGLEQYDARRSAGNTNDLRGKILRI
KVNEDGTYSIPDGNLFSKNDPKARPEIYVMGNRNPYRISVDQKNNFLYWGEVGPDASNDD
ALRGPRGYDEVNQARKAGNFGWPLFVGNNYAYKAYDYTNGANGNAFDPESPINNSPNNTG
LEKLPAAQPAFIWYPYGASKEFPQVGAGGRTAMAGPVYYATANSPYPAYYNGKLIIYEWV
RGWVKAVTMNAQGDYLSMEPFMAKVSLAAPIDMELGPDGKIYVLEYGKGWFTKNPDAAIT
RIDYLKGNRPPTVEDLNIAKTSGLLPYKMTATVTAKDPDGDALTYIWNLGKGVTKTTTIP
TIQHTYTKAGEYPLSVTVMDKSKASAKSQVIPVFAGNEHPNVNIDLAGNKSFYFPNKPVD
YKVLVSDKGAKVNNSRIYISNTYTEGSDLAGAQLGHQQAAQTLVGKTIMLKSDCSTCHKE
AAVSIGPAFNKIAAKYKNDSKAVDYLASKVIAGSHGVWGEVPMPAHTTMKEAEVKKITEW
IMTLGNKEAVKASLPTSGKIVPPAATDKKKSVLTLKATYTDAGAAGLKPLTTTNLINLKS
SVIDADDIKDFGGFQRKDVFGGEKLVLTKDNGWLALKNIDLTGISGFGFSFLNLDPGADG
EIEIRLDDSKGIMIGKSTFRKSIPINMLSDGKFHSIYFVFKELKTSDKKNRPIIQSITVE
PK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory