SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing CA265_RS12005 CA265_RS12005 glutathione-dependent formaldehyde dehydrogenase to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

4cpdA Alcohol dehydrogenase tadh from thermus sp. Atn1
35% identity, 100% coverage: 1:384/385 of query aligns to 1:346/346 of 4cpdA

query
sites
4cpdA

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active site: C38 (= C38), G39 (= G39), S40 (= S40), H43 (= H43), H59 (= H59), E60 (= E60), C89 (= C89), C92 (= C92), C95 (= C95), C103 (= C103), G107 (≠ N107), D152 (= D169), T156 (= T173), K340 (≠ C378)
binding nicotinamide-adenine-dinucleotide: G39 (= G39), S40 (= S40), T156 (= T173), G178 (= G195), P179 (= P196), V180 (= V197), D200 (= D217), R201 (≠ P218), R205 (= R222), A243 (= A261), V244 (= V262), V266 (≠ M304), V268 (= V306), L292 (≠ Q330), A293 (= A331), F333 (= F371)
binding zinc ion: C38 (= C38), H59 (= H59), C89 (= C89), C92 (= C92), C95 (= C95), C103 (= C103), D152 (= D169)

5ylnA Zinc dependent alcohol dehydrogenase 2 from streptococcus pneumonia - apo form
32% identity, 100% coverage: 1:384/385 of query aligns to 5:342/348 of 5ylnA

query
sites
5ylnA

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active site: C41 (= C38), T43 (≠ S40), H46 (= H43), H63 (= H59)
binding zinc ion: C41 (= C38), H63 (= H59), C93 (= C89), C96 (= C92), C99 (= C95), C107 (= C103)

4jlwA Crystal structure of formaldehyde dehydrogenase from pseudomonas aeruginosa (see paper)
33% identity, 96% coverage: 2:372/385 of query aligns to 3:379/395 of 4jlwA

query
sites
4jlwA

K

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binding nicotinamide-adenine-dinucleotide: C45 (= C38), G46 (= G39), S47 (= S40), H50 (= H43), D168 (= D169), T172 (= T173), G194 (= G195), P195 (= P196), V196 (= V197), D216 (= D217), R221 (= R222), V261 (= V262), R266 (≠ T277), H268 (= H279), V281 (= V287), P298 (≠ M304), L300 (≠ V306), Q336 (= Q330), T337 (≠ A331)
binding zinc ion: C45 (= C38), H66 (= H59), C96 (= C89), C99 (= C92), C102 (= C95), C110 (= C103), D168 (= D169)

1kolA Crystal structure of formaldehyde dehydrogenase (see paper)
32% identity, 96% coverage: 2:372/385 of query aligns to 3:379/396 of 1kolA

query
sites
1kolA

K

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L

K

K

V

V

T

V

S

S

T

T

A

N

I

I

C
|
C

G
|
G

S
|
S

D

D

L

Q

H
|
H

I

M

L

V

S

R

G

G

A

R

V

T

P

T

Q

A

K

Q

D

V

P

G

L

L

I

V

M

L

G

G

H
|
H

E
|
E

F

I

M

T

G

G

I

E

V

V

E

I

E

E

V

K

G

G

A

R

S

D

I

V

T

E

N

N

L

L

K

Q

R

I

G

G

D

D

R

L

V

V

V

S

V

V

P

P

F
|
F

P

N

I

V

S

A

C
|
C

G

G

K

R

C
|
C

F

R

F

S

C
|
C

T

K

H

E

E

M

A

H

S

T

P

G

A

V

C
|
C

E

L

T

T

S

V

N
|
N

F

-

K

-

N

-

Y

-

G

-

P

-

N

-

G

-

D

-

M

-

S

P

Q

A

K

R

G

A

A

G

L

A

F

Y

G

G

Y

Y

T

V

D

D

L

M

Y

-

G

G

G

D

Y

W

S

T

G

G

Q

Q

A

A

Q

E

Y

Y

V

V

R

L

V

V

P

P

Y

Y

A

A

D

D

I

F

S

N

P

L

R

L

I

K

V

L

P

P

E

D

H

R

L

D

S

K

D

A

E

M

Q

E

A

K

L

I

-

R

-

D

-

L

-

T

F

C

L

L

T

S

D
|
D

I

I

F

L

P

P

T
|
T

G

G

W

Y

S

H

A

G

I

A

D

V

W

T

A

A

Q

G

L

V

K

G

G

P

G

G

E

S

V

T

V

V

A

Y

I

V

F

A

G
|
G

S

A

G
|
G

P
|
P

V
|
V

G

G

L

L

M

A

A

A

Q

A

K

A

A

S

A

A

W

R

I

L

N

L

G

G

A

A

S

A

R

V

V

V

I

I

A

V

I

G

D
|
D

P
x
L

L

N

D

P

Y

A

R
|
R

L

L

E

A

K

H

A

A

K

K

A

A

-

Q

-

G

-

F

-

E

V

I

N

A

N

D

V

L

D

S

I

L

L

D

N

T

P

P

H

-

K

-

V

-

D

-

V

L

V

H

E

E

A

Q

I

I

R

A

E

A

M

L

T

L

G

G

G

E

R

P

G

E

A

V

D

D

L

C

C

A

V

V

D

D

A
|
A

V
|
V

G

G

F

F

E

E

P

A

E
x
R

R

G

S
x
H

F

G

F

H

D

E

K

G

V

A

K

K

A

-

T

-

V

-

H

-

F

-

E

H

K

E

G

A

S

P

I

A

K

T

V
|
V

L

L

E

N

M

S

C

L

F

M

E

Q

A

V

V

T

R

R

R

V

M

A

G

G

T

K

V

I

S

G

I

I

M
x
P

G

G

V
x
L

Y

Y

-

V

-

T

-

E

-

D

-

P

-

G

-

A

-

V

-

D

-

A

-

K

-

I

G

G

S

S

P

L

Y

S

D

I

N

R

F

F

P

G

L

L

F

G

R

-

I

-

F

W

D

A

K

K

G

S

I

H

T

S

I

F

K

H

Q

T

G

G

Q
|
Q

A
x
T

P

P

V

V

L

M

N

K

Y

Y

I

N

D

R

K

A

L

L

I

M

G

Q

L

A

V

I

N

M

E

W

G

D

K

R

V

I

V

N

L

I

D

A

D

E

I

V

-

G

T

V

H

Q

T

V

L

I

P

S

L

L

E

D

D

D

A

A

A

P

H

R

G

G

Y

Y

K

G

I

E

F

F

D

D

active site: C45 (= C38), G46 (= G39), S47 (= S40), H50 (= H43), H66 (= H59), E67 (= E60), C96 (= C89), C99 (= C92), C102 (= C95), C110 (= C103), N114 (= N107), D168 (= D169), T172 (= T173)
binding nicotinamide-adenine-dinucleotide: C45 (= C38), G46 (= G39), S47 (= S40), H50 (= H43), F92 (= F85), D168 (= D169), T172 (= T173), G192 (= G193), G194 (= G195), P195 (= P196), V196 (= V197), D216 (= D217), L217 (≠ P218), R221 (= R222), A260 (= A261), V261 (= V262), R266 (≠ E267), H268 (≠ S269), V281 (= V287), P298 (≠ M304), L300 (≠ V306), Q336 (= Q330), T337 (≠ A331)
binding zinc ion: C45 (= C38), H66 (= H59), C96 (= C89), C99 (= C92), C102 (= C95), C110 (= C103), D168 (= D169)

Sites not aligning to the query:

active site: 385

P46154 Glutathione-independent formaldehyde dehydrogenase; FALDH; FDH; Formaldehyde dismutase; EC 1.2.1.46; EC 1.2.98.1 from Pseudomonas putida (Arthrobacter siderocapsulatus) (see paper)
31% identity, 96% coverage: 2:372/385 of query aligns to 5:381/399 of P46154

query
sites
P46154

K

R

A

G

A

V

V

V

F

Y

H

L

K

G

P

S

G

G

D

K

I

V

R

E

V

V

D

Q

Y

K

V

I

P

D

D

Y

P

P

K

K

I

M

L

Q

D

D

P

P

R

R

D

G

-

K

-

I

-

E

-

H

-

G

V

V

I

I

L

L

K

K

V

V

T

V

S

S

T

T

A

N

I

I

C
|
C

G
|
G

S
|
S

D
|
D

L
x
Q

H
|
H

I

M

L

V

S

R

G

G

A

R

V

T

P

T

Q

A

K

Q

D

V

P

G

L

L

I

V

M

L

G

G

H
|
H

E

E

F

I

M

T

G

G

I

E

V

V

E

I

E

E

V

K

G

G

A

R

S

D

I

V

T

E

N

N

L

L

K

Q

R

I

G

G

D

D

R

L

V

V

V

S

V

V

P

P

F

F

P

N

I

V

S

A

C
|
C

G

G

K

R

C
|
C

F

R

F

S

C
|
C

T

K

H

E

E

M

A

H

S

T

P

G

A

V

C
|
C

E

L

T

T

S

V

N

N

F

-

K

-

N

-

Y

-

G

-

P

-

N

-

G

-

D

-

M

-

S

P

Q

A

K

R

G

A

A

G

L

A

F

Y

G

G

Y

Y

T

V

D

D

L

M

Y

-

G

G

G

D

Y

W

S

T

G

G

Q

Q

A

A

Q

E

Y

Y

V

L

R

L

V

V

P

P

Y

Y

A

A

D

D

I

F

S

N

P

L

R

L

I

K

V

L

P

P

E

D

H

R

L

D

S

K

D

A

E

M

Q

E

A

K

L

I

-

R

-

D

-

L

-

T

F

C

L

L

T

S

D

D

I

I

F

L

P

P

T

T

G

G

W

Y

S

H

A

G

I

A

D

V

W

T

A

A

Q

G

L

V

K

G

G

P

G

G

E

S

V

T

V

V

A

Y

I

V

F

A

G

G

S

A

G

G

P

P

V
|
V

G

G

L

L

M

A

A

A

Q

A

K

A

A

S

A

A

W

R

I

L

N

L

G

G

A

A

S

A

R

V

V

V

I

I

A

V

I

G

D
|
D

P

L

L

N

D

P

Y

A

R
|
R

L

L

E

A

K

H

A

A

K

K

A

A

-

Q

-

G

-

F

-

E

V

I

N

A

N

D

V

L

D

S

I

L

L

D

N

T

P

P

H

-

K

-

V

-

D

-

V

L

V

H

E

E

A

Q

I

I

R

A

E

A

M

L

T

L

G

G

G

E

R

P

G

E

A

V

D

D

L

C

C

A

V

V

D

D

A

A

V
|
V

G

G

F

F

E

E

P

A

E
x
R

R
x
G

S
x
H

F

G

F

H

D

E

K

G

V

A

K

K

A

-

T

-

V

-

H

-

F

-

E

H

K

E

G

A

S

P

I

A

K

T

V

V

L

L

E

N

M

S

C

L

F

M

E

Q

A

V

V

T

R

R

R

V

M

A

G

G

T

K

V

I

S

G

I

I

M
x
P

G
|
G

V
x
L

Y

Y

-

V

-

T

-

E

-

D

-

P

-

G

-

A

-

V

-

D

-

A

-

K

-

I

G

G

S

S

P

L

Y

S

D

I

N

R

F

F

P

G

L

L

F

G

R

-

I

-

F

W

D

A

K

K

G

S

I

H

T

S

I

F

K

H

Q

T

G
|
G

Q
|
Q

A
x
T

P

P

V

V

L

M

N

K

Y

Y

I

N

D

R

K

A

L

L

I

M

G

Q

L

A

V

I

N

M

E

W

G

D

K

R

V

I

V

N

L

I

D

A

D

E

I

V

-

G

T

V

H

Q

T

V

L

I

P

S

L

L

E

D

D

D

A

A

A

P

H

R

G

G

Y

Y

K

G

I

E

F

F

D

D

C47 (= C38) binding
GSDQH 48:52 (≠ GSDLH 39:43) binding
H68 (= H59) binding
C98 (= C89) binding
C101 (= C92) binding
C104 (= C95) binding
C112 (= C103) binding
V198 (= V197) binding
D218 (= D217) binding
R223 (= R222) binding
V263 (= V262) binding
RGH 268:270 (≠ ERS 267:269) binding
PGL 300:302 (≠ MGV 304:306) binding
GQT 337:339 (≠ GQA 329:331) binding

2dphA Crystal structure of formaldehyde dismutase
34% identity, 96% coverage: 2:372/385 of query aligns to 4:380/398 of 2dphA

query
sites
2dphA

K

K

A

S

A

V

V

V

F

Y

H

H

K

G

P

T

G

R

D

D

I

L

R

R

V

V

D

E

Y

T

V

V

P

P

D

Y

P

P

K

K

I

L

L

E

D

H

-

N

-

R

-

K

-

L

P

E

R

H

D

A

V

V

I

I

L

L

K

K

V

V

T

V

S

S

T

T

A

N

I

I

C
|
C

G
|
G

S
|
S

D

D

L

Q

H
|
H

I

I

L

Y

S

R

G

G

A

R

V

F

P

I

Q

V

K

P

D

K

P

G

L

H

I

V

M

L

G

G

H
|
H

E
|
E

F

I

M

T

G

G

I

E

V

V

E

V

E

E

V

K

G

G

A

S

S

D

I

V

T

E

N

L

L

M

K

D

R

I

G

G

D

D

R

L

V

V

V

S

V

V

P

P

F
|
F

P

N

I

V

S

A

C
|
C

G

G

K

R

C
|
C

F

R

F

N

C
|
C

T

K

H

E

E

A

A

R

S

S

P

D

A

V

C
|
C

E

E

T

-

S

N

N

N

F
x
L

K

V

N

N

Y

-

G

-

P

P

N

D

G

A

D

D

M

L

M

-

S

-

Q

-

K

-

G

-

A

-

A

G

L

A

F

F

G

G

Y

F

T

-

D

D

L

L

Y

K

G

-

G

G

Y

W

S

S

G

G

Q

Q

A

A

Q

E

Y

Y

V

V

R

L

V

V

P

P

Y

Y

A

A

D

D

I

Y

S

-

P

-

R

-

I

M

V

L

P

L

E

K

H

F

L

G

S

D

D

K

E

E

Q

Q

A

A

L

M

-

E

-

K

-

I

-

K

-

D

-

L

-

T

F

L

L

I

T
x
S

D
|
D

I

I

F

L

P
|
P

T
|
T

G

G

W

F

S

H

A

G

I

C

D

V

W

S

A

A

Q

G

L

V

K

K

G

P

G

G

E

S

V

H

V

V

A

Y

I

I

F
x
A

G
|
G

S

A

G
|
G

P
|
P

V
|
V

G

G

L

R

M

C

A

A

Q

A

K

A

A

G

A

A

W

R

I

L

N

L

G

G

A

A

S

A

R

C

V

V

I

I

A

V

I
x
G

D
|
D

-
x
Q

-

N

P

P

L

E

D
x
R

Y

L

R

K

L

L

E

L

K

S

A

D

K

A

A

G

V

F

N

E

N

T

V

I

D

D

I
x
L

L

R

N

N

P

-

H

-

K

S

V

A

D

P

V

L

V

R

E

D

A

Q

I

I

R

D

E

Q

M

I

T

L

G

G

G

K

R

P

G

E

A

V

D

D

L

C

C

G

V

V

D

D

A

A

V
|
V

G

G

F

F

E

E

P

-

E

A

R
x
H

S

G

F

L

F

G

D

D

K

-

V

-

K

E

A

A

T

N

V

T

H

E

F

T

E

P

K

N

G

G

S

A

I

L

K

N

V

S

L

L

E

-

M

-

C

-

F

F

E

D

A

V

V

V

R

R

R

A

M

G

G

G

T

A

V

I

S

G

I

I

M
x
P

G
|
G

V
x
I

Y

Y

-

V

-

G

-

S

-

D

-

P

-

D

-

P

-

V

-

N

-

K

-

D

-

A

G

G

S

S

P

G

Y

R

D

L

N

H

F

L

P

D

L

F

F

G

R

K

I

M

F

W

D

T

K

K

G

S

I

I

T

R

I

I

K

M

Q

T

G

G

Q

M

A
|
A

P

P

V

V

L

T

N

N

Y

Y

I

N

D

R

K

H

L

L

I

T

G

E

L

A

V

I

-

L

-

W

-

D

-

Q

-

M

-

P

N

Y

E

L

G

S

K

K

V

V

V

M

L

N

D

I

D

E

I

V

I

I

T

T

H

-

T

-

L

-

P

-

L

L

E

D

D

Q

A

A

A

P

H

D

G

G

Y

Y

K

A

I

K

F

F

D

D

active site: C45 (= C38), G46 (= G39), S47 (= S40), H50 (= H43), H66 (= H59), E67 (= E60), C96 (= C89), C99 (= C92), C102 (= C95), C110 (= C103), L114 (≠ F108), S168 (≠ T168), D169 (= D169), P172 (= P172)
binding nicotinamide-adenine-dinucleotide: C45 (= C38), G46 (= G39), S47 (= S40), H50 (= H43), F92 (= F85), D169 (= D169), T173 (= T173), A192 (≠ F192), G193 (= G193), G195 (= G195), P196 (= P196), V197 (= V197), G216 (≠ I216), D217 (= D217), Q218 (vs. gap), R222 (≠ D220), L236 (≠ I234), V262 (= V262), H267 (≠ R268), P298 (≠ M304), G299 (= G305), I300 (≠ V306), A337 (= A331)
binding zinc ion: C45 (= C38), H66 (= H59), C96 (= C89), C99 (= C92), C102 (= C95), C110 (= C103), D169 (= D169)

Sites not aligning to the query:

active site: 386

Q52078 Formaldehyde dismutase; EC 1.2.98.1 from Pseudomonas putida (Arthrobacter siderocapsulatus) (see paper)
34% identity, 96% coverage: 2:372/385 of query aligns to 5:381/399 of Q52078

query
sites
Q52078

K
|
K

A
x
S

A
x
V

V
|
V

F
x
Y

H
|
H

K
x
G

P
x
T

G
x
R

D
|
D

I
x
L

R
|
R

V
|
V

D
x
E

Y
x
T

V
|
V

P
|
P

D
x
Y

P
|
P

K
|
K

I
x
L

L
x
E

D
x
H

-
x
N

-
x
R

-
x
K

-
x
L

P
x
E

R
x
H

D
x
A

V
|
V

I
|
I

L
|
L

K
|
K

V
|
V

T
x
V

S
|
S

T
|
T

A
x
N

I
|
I

C
|
C

G
|
G

S
|
S

D
|
D

L
x
Q

H
|
H

I
|
I

L
x
Y

S
x
R

G
|
G

A
x
R

V
x
F

P
x
I

Q
x
V

K
x
P

D
x
K

P
x
G

L
x
H

I
x
V

M
x
L

G
|
G

H
|
H

E
|
E

F
x
I

M
x
T

G
|
G

I
x
E

V
|
V

E
x
V

E
|
E

V
x
K

G
|
G

A
x
S

S
x
D

I
x
V

T
x
E

N
x
L

L
x
M

K
x
D

R
x
I

G
|
G

D
|
D

R
x
L

V
|
V

V
x
S

V
|
V

P
|
P

F
|
F

P
x
N

I
x
V

S
x
A

C
|
C

G
|
G

K
x
R

C
|
C

F
x
R

F
x
N

C
|
C

T
x
K

H
x
E

E
x
A

A
x
R

S
|
S

P
x
D

A
x
V

C
|
C

E
|
E

T

-

S
x
N

N
|
N

F
x
L

K
x
V

N
|
N

Y

-

G

-

P
|
P

N
x
D

G
x
A

D
|
D

M
x
L

M

-

S

-

Q

-

K

-

G

-

A

-

A
x
G

L
x
A

F
|
F

G
|
G

Y
x
F

T

-

D
|
D

L
|
L

Y
x
K

G

-

G
|
G

Y
x
W

S
|
S

G
|
G

Q
|
Q

A
|
A

Q
x
E

Y
|
Y

V
|
V

R
x
L

V
|
V

P
|
P

Y
|
Y

A
|
A

D
|
D

I
x
Y

S

-

P

-

R

-

I
x
M

V
x
L

P
x
L

E
x
K

H
x
F

L
x
G

S
x
D

D
x
K

E
|
E

Q
|
Q

A
|
A

L
x
M

-
x
E

-
x
K

-
x
I

-
x
K

-
x
D

-
x
L

-
x
T

F
x
L

L
x
I

T
x
S

D
|
D

I
|
I

F
x
L

P
|
P

T
|
T

G
|
G

W
x
F

S
x
H

A
x
G

I
x
C

D
x
V

W
x
S

A
|
A

Q
x
G

L
x
V

K
|
K

G
x
P

G
|
G

E
x
S

V
x
H

V
|
V

A
x
Y

I
|
I

F
x
A

G
|
G

S
x
A

G
|
G

P
|
P

V
|
V

G
|
G

L
x
R

M
x
C

A
|
A

Q
x
A

K
x
A

A
x
G

A
|
A

W
x
R

I
x
L

N
x
L

G
|
G

A
|
A

S
x
A

R
x
C

V
|
V

I
|
I

A
x
V

I
x
G

D
|
D

-
x
Q

-
x
N

P
|
P

L
x
E

D
x
R

Y
x
L

R
x
K

L
|
L

E
x
L

K
x
S

A
x
D

K
x
A

A
x
G

V
x
F

N
x
E

N
x
T

V
x
I

D
|
D

I
x
L

L
x
R

N
|
N

P

-

H

-

K
x
S

V
x
A

D
x
P

V
x
L

V
x
R

E
x
D

A
x
Q

I
|
I

R
x
D

E
x
Q

M
x
I

T
x
L

G
|
G

G
x
K

R
x
P

G
x
E

A
x
V

D
|
D

L
x
C

C
x
G

V
|
V

D
|
D

A
|
A

V
|
V

G
|
G

F
|
F

E
|
E

P

-

E
x
A

R
x
H

S
x
G

F
x
L

F
x
G

D
|
D

K

-

V

-

K
x
E

A
|
A

T
x
N

V
x
T

H
x
E

F
x
T

E
x
P

K
x
N

G
|
G

S
x
A

I
x
L

K
x
N

V
x
S

L
|
L

E

-

M

-

C

-

F
|
F

E
x
D

A
x
V

V
|
V

R
|
R

R
x
A

M
x
G

G
|
G

T
x
A

V
x
I

S
x
G

I
|
I

M
x
P

G
|
G

V
x
I

Y
|
Y

-
x
V

-
x
G

-
x
S

-
x
D

-
x
P

-
x
D

-
x
P

-
x
V

-
x
N

-
x
K

-
x
D

-
x
A

G
|
G

S
|
S

P
x
G

Y
x
R

D
x
L

N
x
H

F
x
L

P
x
D

L
x
F

F
x
G

R
x
K

I
x
M

F
x
W

D
x
T

K
|
K

G
x
S

I
|
I

T
x
R

I
|
I

K
x
M

Q
x
T

G
|
G

Q
x
M

A
|
A

P
|
P

V
|
V

L
x
T

N
|
N

Y
|
Y

I
x
N

D
x
R

K
x
H

L
|
L

I
x
T

G
x
E

L
x
A

V
x
I

-
x
L

-
x
W

-
x
D

-
x
Q

-
x
M

-
x
P

N
x
Y

E
x
L

G
x
S

K
|
K

V
|
V

V
x
M

L
x
N

D
x
I

D
x
E

I
x
V

I
|
I

T
|
T

H

-

T

-

L

-

P

-

L
|
L

E
x
D

D
x
Q

A
|
A

A
x
P

H
x
D

G
|
G

Y
|
Y

K
x
A

I
x
K

F
|
F

D
|
D

C46 (= C38) binding
GSDQH 47:51 (≠ GSDLH 39:43) binding
H67 (= H59) binding
C97 (= C89) binding
C100 (= C92) binding
C103 (= C95) binding
C111 (= C103) binding
D170 (= D169) binding
T174 (= T173) binding
PV 197:198 (= PV 196:197) binding
DQ 218:219 (≠ D- 217) binding
R223 (≠ D220) binding
V263 (= V262) binding
H268 (≠ R268) binding
P299 (≠ M304) binding
PGI 299:301 (≠ MGV 304:306) binding
GMA 336:338 (≠ GQA 329:331) binding

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2:399 modified: mature protein, Formaldehyde dismutase

O58389 L-threonine 3-dehydrogenase; L-ThrDH; TDH; L-threonine dehydrogenase; EC 1.1.1.103 from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) (see 2 papers)
31% identity, 98% coverage: 1:376/385 of query aligns to 5:330/348 of O58389

query
sites
O58389

M

M

K

V

A

A

A

I

V

M

F

K

H

T

K

K

P

P

G

G

D

Y

-

G

-

A

-

E

-

L

I

V

R

E

V

V

D

D

Y

-

V

V

P

P

D

K

P

P

K

-

I

-

L

-

D

G

P

P

R

G

D

E

V

V

I

L

L

I

K

K

V

V

T

L

S

A

T

T

A

S

I

I

C
|
C

G

G

S
x
T

D

D

L

L

H

H

I

I

L

Y

S

E

-

W

-

N

-

E

-

W

G

A

A

Q

V

S

P

R

Q

I

K

K

D

P

P

P

L

Q

I

I

M

M

G

G

H
|
H

E
|
E

F

V

M

A

G

G

I

E

V

V

E

V

E

E

V

I

G

G

A

P

S

G

I

V

T

E

N

G

L

I

K

E

R

V

G

G

D

D

R

Y

V

V

V

S

V

V

P

E

F

T

P

H

I

I

S

V

C
|
C

G

G

K

K

C
|
C

F

Y

F

A

C
|
C

T

R

H

R

E

G

A

Q

S

Y

P

H

A

V

C
|
C

E

Q

T

-

S

-

N

N

F

T

K

K

N

I

Y

F

G

G

P

V

N

D

G

T

D

D

M

-

S

-

Q

-

K

-

G

-

A

-

L

-

F

-

G

-

Y

-

T

-

D

-

L

-

Y

-

G

-

Y

-

S

-

G

G

G

V

Q

F

A

A

Q

E

Y

Y

V

A

R

V

V

V

P

P

Y

A

A

Q

D

N

I

I

-

W

-

K

S

N

P

P

R

K

I

S

V

I

P

P

E

P

H

E

L

Y

S

A

D

T

E

L

Q

Q

A
x
E

L

-

F

-

L

-

T

-

D

-

I

-

F

-

P

P

T

L

G

G

W

-

S

N

A

A

I

V

D

D

W

-

A

T

Q

V

L

L

K

A

G

G

-

P

-

I

-

S

G

G

E

K

V

S

V

V

A

L

I

I

F

T

G

G

S

A

G

G

P

P

V
x
L

G

G

L

L

M

L

A

G

Q

I

K

A

A

V

A

A

W

K

I

A

N

S

G

G

A

A

S

Y

R

P

V

V

I

I

A

V

I

S

D
x
E

P

P

L

S

D

D

Y

F

R
|
R

L

R

E

E

K

L

A

A

K

K

A

K

V

V

N

G

N

A

V

D

D

Y

I

V

L

I

N

N

P

P

H

F

K

E

V

E

D

D

V

V

E

K

A

E

I

V

R

M

E

D

M

I

T

T

G

D

G

G

R

N

G

G

A

V

D

D

L

V

C

F

V

L

D

E

A

F

V

-

G

-

F

-

E

-

P

-

E

-

R

-

S

-

F

-

D

-

K

-

V

-

K

-

A

-

T

-

V

-

H

-

F

-

E

-

K

S

G

G

S

A

I

P

K

K

V

A

L

L

E

E

M

Q

C

G

F

L

E

Q

A

A

V

V

R

T

R

P

M

A

G

G

T

R

V

V

S

S

I

L

M
x
L

G
|
G

V
x
L

Y

Y

G

P

S

G

P

K

-

V

-

T

-

I

-

D

Y

F

D

N

N

N

F

L

P

I

L

I

F

F

R

K

I

A

F

L

D

T

-

I

K

Y

G

G

I
|
I

T
|
T

I

G

K
x
R

Q

H

G

-

Q

-

A

-

P

-

V

L

L

W

N

E

Y

T

I

W

D

Y

K

T

L

V

I

S

G

R

L

L

V

L

N

Q

E

S

G

G

K

K

V

L

V

N

L

L

D

D

D

P

I

I

T

T

H

H

T

K

L

-

P

-

L

-

E

-

D

-

A

-

H

-

G

-

Y

Y

K

K

I

G

F

F

D

D

N

K

K

Y

E

E

C42 (= C38) binding
T44 (≠ S40) mutation to A: Total loss of enzymatic activity.
H67 (= H59) binding
E68 (= E60) binding
C97 (= C89) binding
C100 (= C92) binding
C103 (= C95) binding
C111 (= C103) binding
E152 (≠ A164) mutation E->A,Q: Almost complete loss of enzymatic activity.; mutation to C: 120-fold decrease in catalytic efficiency.; mutation to D: Shows 3-fold higher turnover rate and reduced affinities toward L-threonine and NAD(+), compared to wild-type.; mutation to K: Total loss of enzymatic activity.
L179 (≠ V197) binding
E199 (≠ D217) binding ; mutation to A: Large decrease in affinity for NAD(+).
R204 (= R222) binding ; mutation to A: Large decrease in affinity for NAD(+).
LGL 266:268 (≠ MGV 304:306) binding
IT 291:292 (= IT 324:325) binding
R294 (≠ K327) mutation to A: 4000-fold decrease in catalytic efficiency.

2dfvA Hyperthermophilic threonine dehydrogenase from pyrococcus horikoshii (see paper)
31% identity, 98% coverage: 1:376/385 of query aligns to 3:328/346 of 2dfvA

query
sites
2dfvA

M

M

K

V

A

A

A

I

V

M

F

K

H

T

K

K

P

P

G

G

D

Y

-

G

-

A

-

E

-

L

I

V

R

E

V

V

D

D

Y

-

V

V

P

P

D

K

P

P

K

-

I

-

L

-

D

G

P

P

R

G

D

E

V

V

I

L

L

I

K

K

V

V

T

L

S

A

T

T

A

S

I

I

C
|
C

G
|
G

S
x
T

D

D

L

L

H
|
H

I

I

L

Y

S

E

-

W

-

N

-

E

-

W

G

A

A

Q

V

S

P

R

Q

I

K

K

D

P

P

P

L

Q

I

I

M

M

G

G

H
|
H

E
|
E

F

V

M

A

G

G

I

E

V

V

E

V

E

E

V

I

G

G

A

P

S

G

I

V

T

E

N

G

L

I

K

E

R

V

G

G

D

D

R

Y

V

V

V

S

V

V

P

E

F

T

P

H

I

I

S

V

C
|
C

G

G

K

K

C
|
C

F

Y

F

A

C
|
C

T

R

H

R

E

G

A

Q

S

Y

P

H

A

V

C
|
C

E

Q

T

-

S

-

N

N

F

T

K
|
K

N

I

Y

F

G

G

P

V

N

D

G

T

D

D

M

-

S

-

Q

-

K

-

G

-

A

-

L

-

F

-

G

-

Y

-

T

-

D

-

L

-

Y

-

G

-

Y

-

S

-

G

G

G

V

Q

F

A

A

Q

E

Y

Y

V

A

R

V

V

V

P

P

Y

A

A

Q

D

N

I

I

-

W

-

K

S

N

P

P

R

K

I

S

V

I

P

P

E

P

H

E

L

Y

S

A

D

T

E

L

Q

Q

A

E

L

-

F

-

L

-

T

-

D

-

I

-

F

-

P
|
P

T

L

G

G

W

-

S

N

A
|
A

I

V

D

D

-

T

-

V

W

L

A

A

Q

G

L

P

K

I

G

S

G

G

E

K

V

S

V

V

A

L

I

I

F

T

G

G

S

A

G
|
G

P
|
P

V
x
L

G

G

L

L

M

L

A

G

Q

I

K

A

A

V

A

A

W

K

I

A

N

S

G

G

A

A

S

Y

R

P

V

V

I

I

A

V

I

S

D
x
E

P
|
P

L

S

D

D

Y

F

R
|
R

L

R

E

E

K

L

A

A

K

K

A

K

V

V

N

G

N

A

V

D

D

Y

I

V

L

I

N

N

P

P

H

F

K

E

V

E

D

D

V

V

E

K

A

E

I

V

R

M

E

D

M

I

T

T

G

D

G

G

R

N

G

G

A

V

D

D

L

V

C

F

V

L

D

E

A
x
F

V

-

G

-

F

-

E

-

P

-

E

-

R

-

S

-

F

-

D

-

K

-

V

-

K

-

A

-

T

-

V

-

H

-

F

-

E

-

K
x
S

G

G

S
x
A

I

P

K

K

V

A

L

L

E

E

M

Q

C

G

F

L

E

Q

A

A

V

V

R

T

R

P

M

A

G

G

T

R

V

V

S

S

I

L

M
x
L

G
|
G

V
x
L

Y

Y

G

P

S

G

P

K

-

V

-

T

-

I

-

D

Y

F

D

N

N

N

F

L

P

I

L

I

F

F

R

K

I

A

F

L

D

T

-

I

K

Y

G

G

I
|
I

T
|
T

I

G

K

R

Q

H

G

-

Q

-

A

-

P

-

V

L

L

W

N

E

Y

T

I

W

D

Y

K

T

L

V

I

S

G

R

L

L

V

L

N

Q

E

S

G

G

K

K

V

L

V

N

L

L

D

D

D

P

I

I

T

T

H

H

T

K

L

-

P

-

L

-

E

-

D

-

A

-

H

-

G

-

Y

Y

K

K

I

G

F

F

D

D

N

K

K

Y

E

E

active site: C40 (= C38), G41 (= G39), T42 (≠ S40), H45 (= H43), H65 (= H59), E66 (= E60), C95 (= C89), C98 (= C92), C101 (= C95), C109 (= C103), K113 (= K109), P151 (= P172), A155 (= A177)
binding nicotinamide-adenine-dinucleotide: G175 (= G195), P176 (= P196), L177 (≠ V197), E197 (≠ D217), P198 (= P218), R202 (= R222), F241 (≠ A261), S242 (≠ K282), A244 (≠ S284), L264 (≠ M304), G265 (= G305), L266 (≠ V306), I289 (= I324), T290 (= T325)
binding zinc ion: C95 (= C89), C101 (= C95), C109 (= C103)

Sites not aligning to the query:

active site: 340

P25406 Alcohol dehydrogenase 1B; Alcohol dehydrogenase I-B; ADH IB; EC 1.1.1.1 from Saara hardwickii (Indian spiny-tailed lizard) (Uromastyx hardwickii) (see paper)
29% identity, 97% coverage: 2:373/385 of query aligns to 10:365/375 of P25406

query
sites
P25406

K

K

A

A

V

V

F

V

H

W

K

E

P

P

G

K

D

K

-

P

-

F

-

S

-

I

I

V

R

E

V

I

D

E

Y

V

V

A

P

P

D

-

P

P

K

K

I

A

L

-

D

-

P

-

R

H

D

E

V

V

I

R

L

I

K

K

V

I

T

L

S

A

T

S

A

G

I

I

C

C

G

R

S

S

D

D

L

D

H

H

I

V

L

L

S

S

G

G

A

A

V

L

P

K

Q

V

K

N

D

F

P

P

L

I

I

I

M

L

G

G

H

H

E

E

F

A

M

A

G

G

I

V

V

V

E

E

E

S

V

V

G

G

A

E

S

G

I

V

T

T

N

S

L

M

K

K

R

P

G

G

D

D

R

K

V

V

V

I

V

P

P

I

F

F

P

L

I

P

S

Q

C

C

G

G

K

E

C

C

F

N

F

S

C

C

T

R

H

H

E

P

A

R

S

G

P

N

A

V

C

C

E

K

T

K

S

S

N

E

F

L

K

-

N

-

Y

-

G

G

P

P

N

F

G

T

D

G

M

L

S

Y

Q

D

K

-

G

G

A

T

A

S

L

R

F

F

G

T

Y

Y

T

Q

D

G

-

K

-

P

-

V

L

Y

Y

H

G

F

G

V

Y

R

S

T

G

G

G

T

Q

F

A

T

Q

E

Y

Y

V

T

R

V

V

A

P

P

Y

E

A

D

D

S

I

V

S

V

P

K

R

I

I

D

V

A

P

S

E

A

H

P

L

L

S

-

D

-

E

E

Q

K

A

V

L

C

F

L

L

I

T

G

D

C

I

G

F

F

P

S

T

T

G

G

W

Y

-

G

S

A

A

A

I

I

D

N

W

S

A

A

Q

K

L

V

K

Q

G

P

G

G

E

S

V

T

V

C

A

A

I

V

F

F

G

G

S

L

G

G

P

G

V

V

G

G

L

L

M

S

A

A

Q

V

K

M

A

G

A

C

W

K

I

A

N

A

G

G

A

A

S

S

R

R

V

I

I

I

A

G

I

I

D

D

P

I

L

N

D

K

Y

E

R

K

L

F

E

P

K

K

A

A

K

K

A

E

V

L

N

G

N

A

V

T

D

E

I

C

L

V

N

N

P

P

-

L

-

D

H

Y

K

K

V

K

D

P

V

I

V

N

E

E

A

V

I

L

R

F

E

D

M

M

T

T

G

D

G

G

R

E

G

G

A

V

D

E

L

Y

C

S

V

F

D

E

A

V

V

I

G

G

F

-

E

-

P

-

E

R

R

T

S

D

F

T

F

M

D

T

K

A

V

A

K

L

A

A

T

S

V

C

H

H

F

-

E

-

K

-

G

-

S

-

I

-

K

-

V

-

L

-

E

-

M

-

C

-

F

-

E

-

A

-

V

-

R

N

M

Y

G

G

T

T

V

S

S

V

I

I

M

V

G

G

V

V

Y

P

G

P

S

S

-

A

-

S

-

Q

-

I

P

A

Y

F

D

D

N

P

F

L

P

L

L

L

F

F

R

-

I

-

F

-

D

-

K

T

G

G

I

R

T

T

I

W

K

K

Q

G

-

S

-

V

-

F

G

G

Q

G

A

W

P

K

V

S

L

K

N

D

Y

A

I

V

D

P

K

R

L

L

I

V

G

S

L

D

V

F

N

M

E

G

G

K

K

K

V

F

V

I

L

L

D

D

D

P

I

L

I

I

T

T

H

H

T

T

L

M

P

P

L

F

E

E

D

K

A

I

A

N

H

E

G

G

Y

F

K

E

I

L

F

L

D

R

N

S

Sites not aligning to the query:

1 modified: N-acetylserine

P80512 Alcohol dehydrogenase 1; EC 1.1.1.1 from Naja naja (Indian cobra) (see paper)
29% identity, 97% coverage: 2:373/385 of query aligns to 10:365/375 of P80512

query
sites
P80512

K

K

A

A

V

I

-

A

-

W

-

E

F

L

H

N

K

K

P

P

G

L

D

S

I

I

R

E

V

Q

D

I

Y

E

V

V

P

A

D

P

P

P

K

K

I

-

L

-

D

-

P

A

R

H

D

E

V

V

I

R

L

I

K

K

V

I

T

L

S

A

T

T

A

G

I

V

C

C

G

R

S

S

D

D

L

E

H

H

I

V

L

I

S

S

G

G

A

A

V

F

P

R

Q

M

K

P

D

L

P

P

L

M

I

V

M

L

G

G

H

H

E

E

F

A

M

A

G

G

I

V

V

V

E

E

E

S

V

V

G

G

A

E

S

G

I

V

T

T

N

C

L

V

K

K

R

P

G

G

D

D

R

K

V

V

V

I

V

P

P

L

F

F

P

A

I

P

S

Q

C

C

G

G

K

K

C

C

F

R

F

A

C

C

T

Q

H

S

E

P

A

K

S

G

P

N

A

L

C

C

E

-

T

T

S

S

N

N

F

D

K

L

N

N

Y

S

G

G

-

S

-

G

-

L

-

M

P

P

N

D

G

G

-

T

D

S

M

R

M

F

S

T

Q

C

K

K

G

G

A

K

A

S

L

I

-

H

F

F

G

I

Y

S

T

T

D

S

L

T

Y

F

G

T

G

E

Y

Y

S

T

G

-

Q

-

A

-

Q

-

Y

-

V

-

R

-

V

V

P

V

Y

H

A

E

D

N

I

S

S

V

P

V

R

K

I

I

V

D

P

P

E

S

H

A

L

-

S

P

D

L

E

E

Q

K

A

V

L

C

F

L

L

I

T

G

D

C

I

G

F

F

P

S

T

T

G

G

W

Y

-

G

S

A

A

A

I

M

D

Q

W

T

A

A

Q

K

L

V

K

E

G

P

G

G

E

S

V

I

V

C

A

A

I

V

F

F

G

G

S

L

G

G

P

G

V

V

G

G

L

L

M

S

A

V

Q

V

K

M

A

G

A

C

W

K

I

A

N

A

G

G

A

A

S

S

R

R

V

I

I

I

A

G

I

V

D

D

P

I

L

N

D

K

Y

D

R

K

L

F

E

A

K

K

A

A

K

K

A

E

V

M

N

G

N

A

V

T

D

E

I

C

L

I

N

N

P

P

-

L

-

D

H

F

K

K

V

K

D

P

V

I

V

N

E

E

A

V

I

L

R

F

E

D

M

L

T

T

G

G

R

E

G

G

A

V

D

D

L

Y

C

S

V

F

D

E

A

V

V

I

G

G

F

R

E

T

P

-

E

-

R

-

S

-

F

-

D

-

K

-

V

-

K

-

A

-

T

-

V

-

H

-

F

-

E

-

K

E

G

T

S

M

I

I

K

S

V

A

L

F

E

T

M

S

C

C

F

H

E

Q

A

-

V

-

R

-

R

N

M

L

G

G

T

T

V

S

S

V

I

V

M

V

G

G

V

V

Y

P

G

P

S

N

P

A

-

S

-

M

-

I

-

T

Y

Y

D

N

N

P

F

L

P

M

L

L

F

F

R

T

I

-

F

-

D

-

K

-

G

G

I

R

T

T

I

W

K

K

Q

G

-

C

-

V

-

F

G

G

Q

G

A

W

P

K

V

S

L

K

N

D

Y

S

I

V

D

P

K

K

L

L

I

V

G

S

L

D

V

F

N

M

E

Q

G

K

K

K

V

F

V

V

L

L

D

D

D

P

I

L

I

I

T

T

H

H

T

T

L

L

P

P

L

F

E

E

D

K

A

I

A

N

H

E

G

G

Y

F

K

D

I

L

F

L

D

R

N

S

Sites not aligning to the query:

1 modified: N-acetylserine

P81601 Alcohol dehydrogenase class-3 chain L; Alcohol dehydrogenase class-III chain L; Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH; S-(hydroxymethyl)glutathione dehydrogenase; EC 1.1.1.1; EC 1.1.1.-; EC 1.1.1.284 from Gadus morhua (Atlantic cod) (see 2 papers)
30% identity, 90% coverage: 27:371/385 of query aligns to 35:363/375 of P81601

query
sites
P81601

D

E

V

V

I

R

L

I

K

K

V

I

T

V

S

A

T

T

A

G

I

I

C

C

G

H

S

T

D

D

L

A

H

Y

I

T

L

L

S

S

G

G

A

S

V

D

P

P

Q

E

K

G

D

V

-

F

P

P

L

S

I

V

M

L

G

G

H

H

E

E

F

G

M

A

G

G

I

I

V

V

E

E

E

S

V

V

G

G

A

E

S

G

I

V

T

T

N

K

L

F

K

K

R

S

G

G

D

D

R

A

V

V

V

I

V

P

P

L

F

Y

P

V

I

P

S

Q

C

C

G

G

K

E

C

C

F

K

F

F

C

C

T

K

H

N

E

P

A

K

S

T

P

N

A

L

C

C

E

Q

T

K

-

I

-

R

-

L

S

T

N

Q

F

G

K

K

N

G

Y

L

G

M

P

P

N

N

G

G

-

T

D

S

M

R

M

F

S

S

Q

C

K

N

G

G

A

Q

A

V

L

L

F

F

G

H

Y

F

T

M

D

G

L

-

Y

-

G

-

Y

-

S

S

G

S

G

T

Q

F

A

S

Q

E

Y

Y

V

T

R

V

V

V

P

-

Y

-

A

A

D

E

I

I

S

S

P

L

R

A

I

K

V

V

P

H

E

E

H

K

L

A

S

P

D

L

E

D

Q

K

A

V

L

C

F

L

L

L

T

G

D

C

I

A

F

I

P

S

T

T

G

G

W

Y

-

G

S

A

A

A

I

L

D

N

W

T

A

A

Q

K

L

V

K

E

G

A

G

G

E

S

V

T

V

C

A

A

I

V

F

F

G

G

S

L

G

G

P

A

V

L

G

G

L

L

M

A

A

V

Q

I

K

M

A

G

A

C

W

Q

I

A

N

A

G

G

A

A

S

S

R

R

V

I

I

I

A

A

I

I

D

D

P

V

L

N

D

P

Y

D

R

K

L

F

E

R

K

I

A

A

K

K

A

E

V

F

N

G

N

A

V

T

D

D

I

L

L

V

N

N

P

P

-

K

-

D

H

H

K

S

V

K

D

P

V

V

V

E

E

Q

A

V

I

L

R

V

E

E

M

M

T

T

G

D

G

G

R

-

G

G

A

V

D

D

L

Y

C

S

V

F

D

E

A

C

V

V

G

-

F

-

E

-

P

-

E

-

R

-

S

-

F

-

D

-

K

-

V

-

K

-

A

-

T

-

V

-

H

-

F

-

E

-

K

-

G

G

S

N

I

I

K

A

V

V

L

M

E

R

M

A

C

A

F

L

E

E

A

A

V

C

R

H

R

K

-

G

M

W

G

G

T

T

V

S

S

V

I

I

M

I

G

G

V

V

-

A

Y

A

G

G

S

Q

P

E

Y

I

D

S

N

T

F

R

P

P

L

F

F

Q

R

L

I

V

F

-

D

-

K

T

G

G

I

R

T

T

I

W

K

K

Q

G

-

T

-

A

-

F

G

G

Q

G

A

Y

P

K

V

S

L

V

N

E

Y

S

I

V

D

P

K

K

L

L

I

V

G

E

L

E

V

Y

N

M

E

N

G

K

K

K

V

V

V

K

L

V

D

D

D

E

I

F

I

V

T

T

H

H

T

T

L

L

P

P

L

F

E

E

D

K

A

I

A

H

H

E

G

G

Y

F

K

D

I

L

F

M

Sites not aligning to the query:

1 modified: N-acetylalanine

1u3tA Crystal structure of human alcohol dehydrogenase alpha-alpha isoform complexed with n-cyclopentyl-n-cyclobutylformamide determined to 2.5 angstrom resolution (see paper)
30% identity, 97% coverage: 2:373/385 of query aligns to 10:364/374 of 1u3tA

query
sites
1u3tA

K

K

A

A

V

V

F

L

-

W

-

E

-

L

H

K

K

K

P

P

G

F

D

S

I

I

R

E

V

E

D

V

Y

E

V

V

P

A

D

P

P

P

K

K

I

-

L

-

D

-

P

A

R

H

D

E

V

V

I

R

L

I

K

K

V

M

T

V

S

A

T

V

A

G

I

I

C
|
C

G
|
G

S
x
T

D

D

L

D

H
|
H

I

V

L

V

S

S

G

G

A

T

V

M

P

V

Q

T

K

P

D

L

P

P

L

V

I

I

M

L

G

G

H
|
H

E
|
E

F

A

M

A

G

G

I

I

V

V

E

E

E

S

V

V

G

G

A

E

S

G

I

V

T

T

N

T

L

V

K

K

R

P

G

G

D

D

R

K

V

-

V

V

V

I

P

P

F

L

P
x
A

I
|
I

-

P

S

Q

C
|
C

G

G

K

K

C
|
C

F

R

F

I

C
|
C

T

K

H

N

E

P

A

E

S

S

P

N

A

Y

C
|
C

E

L

T

K

S

N

N
x
D

F

V

K

S

N

N

Y

-

G

-

P

P

N

Q

G

G

D

T

M

L

M

-

S

-

Q

Q

K

D

G

G

A

T

A

S

L

R

F

F

G

T

Y

C

T

R

D

R

L

K

Y

P

G

I

G

H

Y

H

S

F

G

L

G

G

Q

I

A

S

Q

T

Y

F

V

S

R

Q

V

Y

P

T

Y

V

A

V

D

D

I

E

S

N

P

A

R

V

I

A

V

K

P

I

E

D

H

A

L

A

S

S

D

P

-

L

E

E

Q

K

A

V

L

C

F

L

L

I

T

G

D
x
C

I

G

F

F

P

S

T
|
T

G

G

W

Y

-

G

S

S

A

A

I

V

D

N

W

V

A

A

Q

K

L

V

K

T

G

P

G

G

E

S

V

T

V

C

A

A

I

V

F

F

G

G

S
x
L

G
|
G

P

G

V
|
V

G

G

L

L

M

S

A

A

Q

I

K

M

A

G

A

C

W

K

I

A

N

A

G

G

A

A

S

A

R

R

V

I

I

I

A

A

I

V

D
|
D

P
x
I

L

N

D

K

Y

D

R

K

L

F

E

A

K

K

A

A

K

K

A

E

V

L

N

G

N

A

V

T

D

E

I

C

L

I

N

N

P

P

-

Q

-

D

H

Y

K

K

V

K

D

P

V

I

V

Q

E

E

A

V

I

L

R

K

E

E

M

M

T

T

G

D

G

G

R

-

G

G

A

V

D

D

L

F

C

S

V

F

D

E

A

V

V
x
I

G

G

F

-

E

-

P

-

E

-

R

-

S

-

F

R

F

L

D

D

K

T

V

M

K

M

A

A

T

S

V

-

H

-

F

-

E

-

K

-

G

-

S

-

I

-

K

-

V

-

L

L

E

L

M

C

C

C

F

H

E

E

A

A

V

C

R

-

M

-

G

G

T

T

V

S

S

V

I

I

M
x
V

G
|
G

V
|
V

Y

-

G

-

S

-

P

P

Y

P

D

D

N

S

F

Q

P

N

L

L

F

S

R

M

I

N

F

P

D

M

K

L

G

L

I

L

T

T

I

G

K

R

Q

T

G

W

Q

K

A

G

P

A

V
x
I

L

L

-

G

-

F

-

K

-

S

-

K

N

E

Y

C

I

V

D

P

K

K

L

L

I

V

G

A

L

D

V

F

N

M

E

A

G

K

K

K

V

F

V

S

L

L

D

D

D

A

I

L

I

I

T

T

H

H

T

V

L

L

P

P

L

F

E

E

D

K

A

I

A

N

H

E

G

G

Y

F

K

D

I

L

F

L

D

H

N

S

active site: C46 (= C38), G47 (= G39), T48 (≠ S40), H51 (= H43), H67 (= H59), E68 (= E60), C97 (= C89), C100 (= C92), C103 (= C95), C111 (= C103), D115 (≠ N107), C174 (≠ D169), T178 (= T173)
binding cyclobutyl(cyclopentyl)formamide: T48 (≠ S40), H67 (= H59), A93 (≠ P86), I94 (= I87), I318 (≠ V333)
binding nicotinamide-adenine-dinucleotide: C46 (= C38), T48 (≠ S40), H51 (= H43), C174 (≠ D169), T178 (= T173), L200 (≠ S194), G201 (= G195), V203 (= V197), D223 (= D217), I224 (≠ P218), I269 (≠ V262), V292 (≠ M304), G293 (= G305), V294 (= V306)
binding zinc ion: C46 (= C38), H67 (= H59), C97 (= C89), C100 (= C92), C103 (= C95), C111 (= C103), C174 (≠ D169)

Sites not aligning to the query:

active site: 369

1u3vA Crystal structure of human alcohol dehydrogenase beta-1-beta-1 isoform complexed with n-heptylformamide determined to 1.65 angstrom resolution (see paper)
30% identity, 97% coverage: 2:373/385 of query aligns to 10:364/374 of 1u3vA

query
sites
1u3vA

K

K

A

A

V

V

F

L

-

W

-

E

-

V

H

K

K

K

P

P

G

F

D

S

I

I

R

E

V

D

D

V

Y

E

V

V

P

A

D

P

P

P

K

K

I

A

L

Y

D

E

P

V

R

R

D

-

V

-

I

-

L

I

K

K

V

M

T

V

S

A

T

V

A

G

I

I

C
|
C

G
x
R

S
x
T

D

D

L

D

H
|
H

I

V

L

V

S

S

G

G

A

N

V

L

P

V

Q

T

K

P

D

L

P

P

L

V

I

I

M

L

G

G

H
|
H

E
|
E

F

A

M

A

G

G

I

I

V

V

E

E

E

S

V

V

G

G

A

E

S

G

I

V

T

T

N

T

L

V

K

K

R

P

G

G

D

D

R

K

V

V

V

I

V

P

P

L

F
|
F

P

T

I

P

S

Q

C
|
C

G

G

K

K

C
|
C

F

R

F

V

C
|
C

T

K

H

N

E

P

A

E

S

S

P

N

A

Y

C
|
C

E

L

T

K

S

N

N
x
D

F
x
L

K

G

N

N

Y

-

G

-

P

P

N

R

G

G

D

T

M

L

M

-

S

-

Q

Q

K

D

G

G

A

T

A

R

L

R

F

F

G

T

Y

C

T

R

D

G

L

K

Y

P

G

I

G

H

Y

H

S

F

G

L

G

G

Q

T

A

S

Q

T

Y

F

V

S

R

Q

V

Y

P

T

Y

V

A

V

D

D

I

E

S

N

P

A

R

V

I

A

V

K

P

I

E

D

H

A

L

A

S

S

D

P

-

L

E

E

Q

K

A

V

L

C

F

L

L

I

T

G

D
x
C

I

G

F

F

P

S

T
|
T

G

G

W

Y

-

G

S

S

A

A

I

V

D

N

W

V

A

A

Q

K

L

V

K

T

G

P

G

G

E

S

V

T

V

C

A

A

I

V

F

F

G
|
G

S
x
L

G
|
G

P

G

V
|
V

G

G

L

L

M

S

A

A

Q

V

K

M

A

G

A

C

W

K

I

A

N

A

G

G

A

A

S

A

R

R

V

I

I

I

A

A

I

V

D
|
D

P
x
I

L

N

D

K

Y

D

R

K

L

F

E

A

K

K

A

A

K

K

A

E

V

L

N

G

N

A

V

T

D

E

I

C

L

I

N

N

P

P

-

Q

-

D

H

Y

K

K

V

K

D

P

V

I

V

Q

E

E

A

V

I

L

R

K

E

E

M

M

T

T

G

D

G

G

R

-

G

G

A

V

D

D

L

F

C

S

V

F

D

E

A
x
V

V
x
I

G

G

F

-

E

-

P

-

E

-

R

-

S

-

F
x
R

F

L

D

D

K

T

V

M

K

M

A

A

T

S

V

-

H

-

F

-

E

-

K

-

G

-

S

-

I

-

K

-

V

-

L

L

E

L

M

C

C

C

F

H

E

E

A

A

V

C

R

-

M

-

G

G

T

T

V

S

S

V

I

I

M
x
V

G
|
G

V
|
V

Y

P

G

P

S

A

P

S

Y

Q

D

N

N

L

F

S

P

I

L

N

F

P

R

M

I

L

F

L

D

L

K

T

G

G

I

R

T

T

I

W

K

K

-

G

-

A

-
x
V

Q

Y

G

G

Q

G

A

F

P

K

V

S

L

K

N

E

Y

G

I

I

D

P

K

K

L

L

I

V

G

A

L

D

V

F

N

M

E

A

G

K

K

K

V

F

V

S

L

L

D

D

D

A

I

L

I

I

T

T

H

H

T

V

L

L

P

P

L

F

E

E

D

K

A

I

A

N

H

E

G

G

Y

F

K

D

I

L

F

L

D

H

N

S

active site: C46 (= C38), R47 (≠ G39), T48 (≠ S40), H51 (= H43), H67 (= H59), E68 (= E60), C97 (= C89), C100 (= C92), C103 (= C95), C111 (= C103), D115 (≠ N107), C174 (≠ D169), T178 (= T173)
binding heptylformamide: T48 (≠ S40), H67 (= H59), F93 (= F85), L116 (≠ F108), C174 (≠ D169)
binding nicotinamide-adenine-dinucleotide: R47 (≠ G39), T48 (≠ S40), H51 (= H43), C174 (≠ D169), T178 (= T173), G199 (= G193), L200 (≠ S194), G201 (= G195), V203 (= V197), D223 (= D217), I224 (≠ P218), V268 (≠ A261), I269 (≠ V262), R271 (≠ F270), V292 (≠ M304), G293 (= G305), V294 (= V306), V318 (vs. gap)
binding zinc ion: C46 (= C38), H67 (= H59), C97 (= C89), C100 (= C92), C103 (= C95), C111 (= C103), C174 (≠ D169)

Sites not aligning to the query:

active site: 369

1u3uA Crystal structure of human alcohol dehydrogenase beta-1-beta-1 isoform complexed with n-benzylformamide determined to 1.6 angstrom resolution (see paper)
30% identity, 97% coverage: 2:373/385 of query aligns to 10:364/374 of 1u3uA

query
sites
1u3uA

K

K

A

A

V

V

F

L

-

W

-

E

-

V

H

K

K

K

P

P

G

F

D

S

I

I

R

E

V

D

D

V

Y

E

V

V

P

A

D

P

P

P

K

K

I

A

L

Y

D

E

P

V

R

R

D

-

V

-

I

-

L

I

K

K

V

M

T

V

S

A

T

V

A

G

I

I

C
|
C

G
x
R

S
x
T

D

D

L

D

H
|
H

I

V

L

V

S

S

G

G

A

N

V

L

P

V

Q

T

K

P

D

L

P

P

L

V

I

I

M

L

G

G

H
|
H

E
|
E

F

A

M

A

G

G

I

I

V

V

E

E

E

S

V

V

G

G

A

E

S

G

I

V

T

T

N

T

L

V

K

K

R

P

G

G

D

D

R

K

V

V

V

I

V

P

P

L

F
|
F

P

T

I

P

S

Q

C
|
C

G

G

K

K

C
|
C

F

R

F

V

C
|
C

T

K

H

N

E

P

A

E

S

S

P

N

A

Y

C
|
C

E

L

T

K

S

N

N
x
D

F

L

K

G

N

N

Y

-

G

-

P

P

N

R

G

G

D

T

M

L

M

-

S

-

Q

Q

K

D

G

G

A

T

A

R

L

R

F

F

G

T

Y

C

T

R

D

G

L

K

Y

P

G

I

G

H

Y

H

S

F

G

L

G

G

Q

T

A

S

Q

T

Y

F

V

S

R

Q

V

Y

P

T

Y

V

A

V

D

D

I

E

S

N

P

A

R

V

I

A

V

K

P

I

E

D

H

A

L

A

S

S

D

P

-

L

E

E

Q

K

A

V

L

C

F

L

L

I

T

G

D
x
C

I

G

F

F

P

S

T
|
T

G

G

W

Y

-

G

S

S

A

A

I

V

D

N

W

V

A

A

Q

K

L

V

K

T

G

P

G

G

E

S

V

T

V

C

A

A

I

V

F

F

G
|
G

S
x
L

G
|
G

P

G

V
|
V

G

G

L

L

M

S

A

A

Q

V

K

M

A

G

A

C

W

K

I

A

N

A

G

G

A

A

S

A

R

R

V

I

I

I

A

A

I

V

D
|
D

P
x
I

L

N

D

K

Y

D

R
x
K

L

F

E

A

K

K

A

A

K

K

A

E

V

L

N

G

N

A

V

T

D

E

I

C

L

I

N

N

P

P

-

Q

-

D

H

Y

K

K

V

K

D

P

V

I

V

Q

E

E

A

V

I

L

R

K

E

E

M

M

T

T

G

D

G

G

R

-

G

G

A

V

D

D

L

F

C

S

V

F

D

E

A
x
V

V
x
I

G

G

F

-

E

-

P

-

E

-

R

-

S

-

F
x
R

F

L

D

D

K

T

V

M

K

M

A

A

T

S

V

-

H

-

F

-

E

-

K

-

G

-

S

-

I

-

K

-

V

-

L

L

E

L

M

C

C

C

F

H

E

E

A

A

V

C

R

-

M

-

G

G

T

T

V

S

S

V

I

I

M
x
V

G
|
G

V
|
V

Y

P

G

P

S

A

P

S

Y

Q

D

N

N

L

F

S

P

I

L

N

F

P

R

M

I

L

F

L

D

L

K

T

G

G

I

R

T

T

I

W

K

K

-

G

-

A

-
x
V

Q

Y

G

G

Q

G

A

F

P

K

V

S

L

K

N

E

Y

G

I

I

D

P

K

K

L

L

I

V

G

A

L

D

V

F

N

M

E

A

G

K

K

K

V

F

V

S

L

L

D

D

D

A

I

L

I

I

T

T

H

H

T

V

L

L

P

P

L

F

E

E

D

K

A

I

A

N

H

E

G

G

Y

F

K

D

I

L

F

L

D

H

N

S

active site: C46 (= C38), R47 (≠ G39), T48 (≠ S40), H51 (= H43), H67 (= H59), E68 (= E60), C97 (= C89), C100 (= C92), C103 (= C95), C111 (= C103), D115 (≠ N107), C174 (≠ D169), T178 (= T173)
binding n-benzylformamide: T48 (≠ S40), H67 (= H59), F93 (= F85), C174 (≠ D169), V294 (= V306)
binding nicotinamide-adenine-dinucleotide: R47 (≠ G39), T48 (≠ S40), H51 (= H43), C174 (≠ D169), T178 (= T173), G199 (= G193), L200 (≠ S194), G201 (= G195), V203 (= V197), D223 (= D217), I224 (≠ P218), K228 (≠ R222), V268 (≠ A261), I269 (≠ V262), R271 (≠ F270), V292 (≠ M304), G293 (= G305), V294 (= V306), V318 (vs. gap)
binding zinc ion: C46 (= C38), H67 (= H59), C97 (= C89), C100 (= C92), C103 (= C95), C111 (= C103), C174 (≠ D169)

Sites not aligning to the query:

active site: 369
binding nicotinamide-adenine-dinucleotide: 369

1hdxB Three-dimensional structures of three human alcohol dehydrogenase variants: correlations with their functional differences (see paper)
30% identity, 97% coverage: 2:373/385 of query aligns to 10:364/374 of 1hdxB

query
sites
1hdxB

K

K

A

A

V

V

F

L

-

W

-

E

-

V

H

K

K

K

P

P

G

F

D

S

I

I

R

E

V

D

D

V

Y

E

V

V

P

A

D

P

P

P

K

K

I

A

L

Y

D

E

P

V

R

R

D

-

V

-

I

-

L

I

K

K

V

M

T

V

S

A

T

V

A

G

I

I

C
|
C

G
x
R

S
x
T

D

D

L

D

H
|
H

I

V

L

V

S

S

G

G

A

N

V

L

P

V

Q

T

K

P

D

L

P

P

L

V

I

I

M

L

G

G

H
|
H

E
|
E

F

A

M

A

G

G

I

I

V

V

E

E

E

S

V

V

G

G

A

E

S

G

I

V

T

T

N

T

L

V

K

K

R

P

G

G

D

D

R

K

V

V

V

I

V

P

P

L

F

F

P

T

I

P

S

Q

C
|
C

G

G

K

K

C
|
C

F

R

F

V

C
|
C

T

K

H

N

E

P

A

E

S

S

P

N

A

Y

C
|
C

E

L

T

K

S

N

N
x
D

F
x
L

K

G

N

N

Y

-

G

-

P

P

N

R

G

G

D

T

M

L

M

-

S

-

Q

Q

K

D

G

G

A

T

A

R

L

R

F

F

G

T

Y

C

T

R

D

G

L

K

Y

P

G

I

G

H

Y

H

S

F

G

L

G

G

Q

T

A

S

Q

T

Y

F

V

S

R

Q

V

Y

P

T

Y

V

A

V

D

D

I

E

S

N

P

A

R

V

I

A

V

K

P

I

E

D

H

A

L

A

S

S

D

P

-

L

E

E

Q

K

A

V

L

C

F

L

L

I

T

G

D
x
C

I

G

F

F

P

S

T
|
T

G

G

W

Y

-

G

S

S

A

A

I

V

D

N

W

V

A

A

Q

K

L

V

K

T

G

P

G

G

E

S

V

T

V

C

A

A

I

V

F

F

G

G

S
x
L

G
|
G

P
x
G

V
|
V

G

G

L

L

M

S

A

A

Q

V

K

M

A

G

A

C

W

K

I

A

N

A

G

G

A

A

S

A

R

R

V

I

I

I

A

A

I

V

D
|
D

P

I

L

N

D

K

Y

D

R

K

L

F

E

A

K

K

A

A

K

K

A

E

V

L

N

G

N

A

V

T

D

E

I

C

L

I

N

N

P

P

-

Q

-

D

H

Y

K

K

V

K

D

P

V

I

V

Q

E

E

A

V

I

L

R

K

E

E

M

M

T

T

G

D

G

G

R

-

G

G

A

V

D

D

L

F

C

S

V

F

D

E

A

V

V
x
I

G

G

F

-

E

-

P

-

E

-

R

-

S

-

F
x
R

F

L

D

D

K

T

V

M

K

M

A

A

T

S

V

-

H

-

F

-

E

-

K

-

G

-

S

-

I

-

K

-

V

-

L

L

E

L

M

C

C

C

F

H

E

E

A

A

V

C

R

-

M

-

G

G

T

T

V

S

S

V

I

I

M
x
V

G
|
G

V
|
V

Y

P

G

P

S

A

P

S

Y

Q

D

N

N

L

F

S

P

I

L

N

F

P

R

M

I

L

F

L

D

L

K

T

G

G

I

R

T

T

I

W

K

K

-

G

-

A

-
x
V

Q

Y

G

G

Q

G

A

F

P

K

V

S

L

K

N

E

Y

G

I

I

D

P

K

K

L

L

I

V

G

A

L

D

V

F

N

M

E

A

G

K

K

K

V

F

V

S

L

L

D

D

D

A

I

L

I

I

T

T

H

H

T

V

L

L

P

P

L

F

E

E

D

K

A

I

A

N

H

E

G

G

Y

F

K

D

I

L

F

L

D

H

N

S

active site: C46 (= C38), R47 (≠ G39), T48 (≠ S40), H51 (= H43), H67 (= H59), E68 (= E60), C97 (= C89), C100 (= C92), C103 (= C95), C111 (= C103), D115 (≠ N107), C174 (≠ D169), T178 (= T173)
binding cyclohexanol: L116 (≠ F108), V318 (vs. gap)
binding nicotinamide-adenine-dinucleotide: C46 (= C38), R47 (≠ G39), H51 (= H43), T178 (= T173), L200 (≠ S194), G201 (= G195), G202 (≠ P196), V203 (= V197), D223 (= D217), I269 (≠ V262), R271 (≠ F270), V292 (≠ M304), G293 (= G305), V294 (= V306)
binding zinc ion: C46 (= C38), H67 (= H59), C97 (= C89), C100 (= C92), C103 (= C95), C111 (= C103), C174 (≠ D169)

Sites not aligning to the query:

active site: 369
binding nicotinamide-adenine-dinucleotide: 369

1dehA Crystallization of human beta1 alcohol dehydrogenase (15 mg/ml) in 50 mm sodium phosphate (ph 7.5), 2.0 mm NAD+ and 1 mm 4-iodopyrazole at 25 oc, 13% (w/v) peg 8000 (see paper)
30% identity, 97% coverage: 2:373/385 of query aligns to 10:364/374 of 1dehA

query
sites
1dehA

K

K

A

A

V

V

F

L

-

W

-

E

-

V

H

K

K

K

P

P

G

F

D

S

I

I

R

E

V

D

D

V

Y

E

V

V

P

A

D

P

P

P

K

K

I

A

L

Y

D

E

P

V

R

R

D

-

V

-

I

-

L

I

K

K

V

M

T

V

S

A

T

V

A

G

I

I

C
|
C

G
x
R

S
x
T

D

D

L

D

H
|
H

I

V

L

V

S

S

G

G

A

N

V

L

P

V

Q

T

K

P

D

L

P

P

L

V

I

I

M

L

G

G

H
|
H

E
|
E

F

A

M

A

G

G

I

I

V

V

E

E

E

S

V

V

G

G

A

E

S

G

I

V

T

T

N

T

L

V

K

K

R

P

G

G

D

D

R

K

V

V

V

I

V

P

P

L

F
|
F

P

T

I

P

S

Q

C
|
C

G

G

K

K

C
|
C

F

R

F

V

C
|
C

T

K

H

N

E

P

A

E

S

S

P

N

A

Y

C
|
C

E

L

T

K

S

N

N
x
D

F
x
L

K

G

N

N

Y

-

G

-

P

P

N

R

G

G

D

T

M

L

M

-

S

-

Q

Q

K

D

G

G

A

T

A

R

L

R

F

F

G

T

Y

C

T

R

D

G

L

K

Y

P

G

I

G

H

Y

H

S

F

G

L

G

G

Q

T

A

S

Q

T

Y

F

V

S

R

Q

V

Y

P

T

Y

V

A

V

D

D

I

E

S

N

P

A

R

V

I

A

V

K

P

I

E

D

H

A

L

A

S

S

D

P

-

L

E

E

Q

K

A

V

L

C

F

L

L

I

T

G

D
x
C

I

G

F

F

P

S

T
|
T

G

G

W

Y

-

G

S

S

A

A

I

V

D

N

W

V

A

A

Q

K

L

V

K

T

G

P

G

G

E

S

V

T

V

C

A

A

I

V

F

F

G

G

S

L

G
|
G

P

G

V
|
V

G

G

L

L

M

S

A

A

Q

V

K

M

A

G

A

C

W

K

I

A

N

A

G

G

A

A

S

A

R

R

V

I

I

I

A

A

I

V

D
|
D

P
x
I

L

N

D

K

Y

D

R

K

L

F

E

A

K

K

A

A

K

K

A

E

V

L

N

G

N

A

V

T

D

E

I

C

L

I

N

N

P

P

-

Q

-

D

H

Y

K

K

V

K

D

P

V

I

V

Q

E

E

A

V

I

L

R

K

E

E

M

M

T

T

G

D

G

G

R

-

G

G

A

V

D

D

L

F

C

S

V

F

D

E

A
x
V

V
x
I

G

G

F

-

E

-

P

-

E

-

R

-

S

-

F

R

F

L

D

D

K

T

V

M

K

M

A

A

T

S

V

-

H

-

F

-

E

-

K

-

G

-

S

-

I

-

K

-

V

-

L

L

E

L

M

C

C

C

F

H

E

E

A

A

V

C

R

-

M

-

G

G

T

T

V

S

S

V

I

I

M
x
V

G

G

V
|
V

Y

P

G

P

S

A

P

S

Y

Q

D

N

N

L

F

S

P

I

L

N

F

P

R

M

I

L

F

L

D

L

K

T

G

G

I

R

T

T

I

W

K

K

-

G

-

A

-

V

Q

Y

G

G

Q

G

A

F

P

K

V

S

L

K

N

E

Y

G

I

I

D

P

K

K

L

L

I

V

G

A

L

D

V

F

N

M

E

A

G

K

K

K

V

F

V

S

L

L

D

D

D

A

I

L

I

I

T

T

H

H

T

V

L

L

P

P

L

F

E

E

D

K

A

I

A

N

H

E

G

G

Y

F

K

D

I

L

F

L

D

H

N

S

active site: C46 (= C38), R47 (≠ G39), T48 (≠ S40), H51 (= H43), H67 (= H59), E68 (= E60), C97 (= C89), C100 (= C92), C103 (= C95), C111 (= C103), D115 (≠ N107), C174 (≠ D169), T178 (= T173)
binding nicotinamide-adenine-dinucleotide: C46 (= C38), R47 (≠ G39), T48 (≠ S40), H51 (= H43), C174 (≠ D169), T178 (= T173), G201 (= G195), V203 (= V197), D223 (= D217), I224 (≠ P218), V268 (≠ A261), I269 (≠ V262), V292 (≠ M304), V294 (= V306)
binding 4-iodopyrazole: T48 (≠ S40), H67 (= H59), F93 (= F85), L116 (≠ F108), C174 (≠ D169)
binding zinc ion: C46 (= C38), H67 (= H59), C97 (= C89), C100 (= C92), C103 (= C95), C111 (= C103)

Sites not aligning to the query:

active site: 369

P80360 Alcohol dehydrogenase class-3; Alcohol dehydrogenase class-III; Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH; S-(hydroxymethyl)glutathione dehydrogenase; EC 1.1.1.1; EC 1.1.1.-; EC 1.1.1.284 from Myxine glutinosa (Atlantic hagfish) (see 2 papers)
29% identity, 96% coverage: 2:371/385 of query aligns to 11:364/376 of P80360

query
sites
P80360

K

K

A

A

V

V

F

A

-

W

-

E

-

A

H

K

K

K

P

P

G

L

D

S

I

L

R

E

V

E

D

I

Y

E

V

V

P

A

D

P

P

P

K

K

I

-

L

-

D

-

P

A

R

H

D

E

V

V

I

R

L

M

K

K

V

V

T

L

S

A

T

T

A

A

I

V

C

C

G

H

S

T

D

D

L

A

H

Y

I

T

L

L

S

S

G

G

A

V

V

D

P

P

Q

E

K

G

D

S

-

F

P

P

L

V

I

V

M

L

G

G

H

H

E

E

F

G

M

A

G

G

I

I

V

V

E

E

E

S

V

V

G

G

A

E

S

G

I

V

T

T

N

K

L

F

K

K

R

P

G

G

D

D

R

S

V

V

V

I

V

P

P

L

F

Y

P

I

I

P

S

Q

C

C

G

G

K

E

C

C

F

K

F

F

C

C

T

L

H

N

E

P

A

K

S

T

P

N

A

L

C

C

E

Q

-

K

-

I

-

R

T

V

S

T

N

Q

F

G

K

K

N

G

Y

M

G

M

P

P

N

D

G

G

-

T

D

S

M

R

M

L

S

T

Q

C

K

R

G

G

A

K

A

S

L

L

F

Y

G

H

Y

F

T

M

D

G

L

-

Y

-

G

-

Y

-

S

A

G

S

G

T

Q

F

A

S

Q

E

Y

Y

V

A

R

V

V

V

P

-

Y

-

A

A

D

D

I

I

S

S

-

L

P

C

R

R

I

V

V

A

P

P

E

E

H

A

L

P

S

P

D

D

E

R

Q

V

A

C

L

L

F

L

L

G

T

C

D

G

I

V

F

-

P

S

T

T

G

G

W

Y

S

G

A

A

-

P

I

L

D

N

W

T

A

A

Q

K

L

V

K

E

G

P

G

G

E

S

V

T

V

C

A

A

I

I

F

F

G

G

S

L

G

G

P

A

V

V

G

G

L

L

M

A

A

A

Q

I

K

M

A

G

A

C

W

R

I

V

N

A

G

G

A

A

S

S

R

R

V

I

I

I

A

A

I

I

D

D

P

R

L

N

D

P

Y

D

R

K

L

F

E

E

K

K

A

A

K

R

A

I

V

F

N

G

N

A

V

T

D

D

I

C

L

V

N

V

P

P

H

D

K

A

V

S

D

D

-

K

-

P

V

I

V

S

E

Q

A

V

I

L

R

G

E

E

M

M

T

T

G

D

G

G

R

-

G

G

A

L

D

D

L

Y

C

T

V

F

D

E

A

C

V

V

G

-

F

-

E

-

P

-

E

-

R

-

S

-

F

-

D

-

K

-

V

-

K

-

A

-

T

-

V

-

H

-

F

-

E

-

K

-

G

G

S

N

I

V

K

G

V

I

L

M

E

R

M

A

C

A

F

L

E

E

A

S

V

C

R

H

R

K

M

G

G

W

T

G

V

V

S

S

-

V

I

I

M

L

G

G

V

V

Y

A

G

G

S

G

P

G

Y

Q

D

E

N

I

F

S

P

T

L

R

F

P

R

F

I

Q

F

L

D

V

K

T

G

G

I

R

T

T

I

W

K

K

Q

G

-

A

-

F

G

G

Q

G

A

W

P

K

V

S

L

V

N

E

Y

S

I

V

D

P

K

K

L

L

I

V

G

D

L

D

V

Y

N

M

E

A

G

G

K

K

V

I

V

M

L

V

D

D

D

E

I

F

I

V

T

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Sites not aligning to the query:

1 modified: N-acetylserine

P80338 Alcohol dehydrogenase 1; Alcohol dehydrogenase I; EC 1.1.1.1 from Struthio camelus (Common ostrich) (see 2 papers)
29% identity, 96% coverage: 2:371/385 of query aligns to 10:362/374 of P80338

query
sites
P80338

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Sites not aligning to the query:

1 modified: N-acetylserine

1u3wA Crystal structure of human alcohol dehydrogenase gamma-2-gamma-2 isoform complexed with n-1-methylheptylformamide determined to 1.45 angstrom resolution (see paper)
30% identity, 96% coverage: 2:371/385 of query aligns to 10:362/374 of 1u3wA

query
sites
1u3wA

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active site: C46 (= C38), R47 (≠ G39), S48 (= S40), H51 (= H43), H67 (= H59), E68 (= E60), C97 (= C89), C100 (= C92), C103 (= C95), C111 (= C103), D115 (≠ N107), C174 (≠ D169), T178 (= T173)
binding 1-methylheptylformamide: S48 (= S40), H67 (= H59), F93 (= F85), C174 (≠ D169)
binding nicotinamide-adenine-dinucleotide: C46 (= C38), R47 (≠ G39), S48 (= S40), H51 (= H43), C174 (≠ D169), T178 (= T173), G199 (= G193), G201 (= G195), V203 (= V197), D223 (= D217), I224 (≠ P218), K228 (≠ R222), V268 (≠ A261), I269 (≠ V262), T274 (≠ K273), V292 (≠ M304), G293 (= G305), V294 (= V306), I318 (vs. gap)
binding zinc ion: C46 (= C38), H67 (= H59), C97 (= C89), C100 (= C92), C103 (= C95), C111 (= C103), C174 (≠ D169)

Sites not aligning to the query:

active site: 369

Query Sequence

>CA265_RS12005 CA265_RS12005 glutathione-dependent formaldehyde dehydrogenase
MKAAVFHKPGDIRVDYVPDPKILDPRDVILKVTSTAICGSDLHILSGAVPQKDPLIMGHE
FMGIVEEVGASITNLKRGDRVVVPFPISCGKCFFCTHEASPACETSNFKNYGPNGDMMSQ
KGAALFGYTDLYGGYSGGQAQYVRVPYADISPRIVPEHLSDEQALFLTDIFPTGWSAIDW
AQLKGGEVVAIFGSGPVGLMAQKAAWINGASRVIAIDPLDYRLEKAKAVNNVDILNPHKV
DVVEAIREMTGGRGADLCVDAVGFEPERSFFDKVKATVHFEKGSIKVLEMCFEAVRRMGT
VSIMGVYGSPYDNFPLFRIFDKGITIKQGQAPVLNYIDKLIGLVNEGKVVLDDIITHTLP
LEDAAHGYKIFDNKEEDCVKVVLKP

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory