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Comparing CA265_RS12755 FitnessBrowser__Pedo557:CA265_RS12755 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
P0CH37 NADP-dependent alcohol dehydrogenase C 2; Ms-ADHC 2; EC 1.1.1.2 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see paper)
55% identity, 100% coverage: 1:347/347 of query aligns to 1:347/349 of P0CH37
- K210 (= K210) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)
P0CH36 NADP-dependent alcohol dehydrogenase C 1; Ms-ADHC 1; EC 1.1.1.2 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see paper)
55% identity, 100% coverage: 1:347/347 of query aligns to 1:347/349 of P0CH36
- K210 (= K210) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)
P9WQC5 NADP-dependent alcohol dehydrogenase C; EC 1.1.1.2 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
52% identity, 100% coverage: 1:347/347 of query aligns to 1:346/346 of P9WQC5
- K209 (= K210) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)
1yqdA Sinapyl alcohol dehydrogenase complexed with NADP+ (see paper)
54% identity, 98% coverage: 6:344/347 of query aligns to 12:350/359 of 1yqdA
- active site: C47 (= C41), H48 (= H42), S49 (= S43), H52 (= H46), H69 (= H63), E70 (= E64), C100 (= C94), C103 (= C97), C106 (= C100), C114 (= C108), I118 (≠ T113), C163 (= C159), T167 (= T163), R345 (= R339)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: C47 (= C41), H48 (= H42), S49 (= S43), H52 (= H46), C163 (= C159), T167 (= T163), G188 (= G183), L189 (= L184), G190 (= G185), G191 (= G186), L192 (= L187), S211 (= S206), T212 (= T207), S213 (= S208), K216 (= K210), T251 (= T245), V252 (= V246), S253 (= S247), V274 (= V268), G275 (= G269), A276 (≠ V270), G299 (= G293), I300 (= I294), N340 (≠ G334), R345 (= R339)
- binding zinc ion: C47 (= C41), H69 (= H63), C100 (= C94), C103 (= C97), C106 (= C100), C114 (= C108), C163 (= C159)
1uufA Crystal structure of a zinc-type alcohol dehydrogenase-like protein yahk
53% identity, 99% coverage: 5:347/347 of query aligns to 2:338/339 of 1uufA
- active site: C38 (= C41), H39 (= H42), S40 (= S43), H43 (= H46), H60 (= H63), E61 (= E64), C91 (= C94), C94 (= C97), C97 (= C100), C105 (= C108), T109 (= T113), C156 (= C159), T160 (= T163), R330 (= R339)
- binding zinc ion: C38 (= C41), H60 (= H63), C91 (= C94), C94 (= C97), C97 (= C100), C105 (= C108), C156 (= C159)
Sites not aligning to the query:
5z0cA Nerol dehydrogenase from persicaria minor (see paper)
53% identity, 98% coverage: 6:344/347 of query aligns to 9:347/367 of 5z0cA
- active site: C44 (= C41), S46 (= S43), H49 (= H46), H66 (= H63), C160 (= C159)
- binding zinc ion: C44 (= C41), H66 (= H63), C97 (= C94), C100 (= C97), C103 (= C100), C111 (= C108), C160 (= C159)
6k3gB Crystal structure of 10-hydroxygeraniol dehydrogenase from cantharanthus roseus in complex with NADP+ (see paper)
54% identity, 98% coverage: 6:345/347 of query aligns to 12:351/356 of 6k3gB
- active site: C47 (= C41), S49 (= S43), H52 (= H46), H69 (= H63), C163 (= C159)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: H48 (= H42), S49 (= S43), H52 (= H46), W58 (= W52), C163 (= C159), T167 (= T163), G188 (= G183), L189 (= L184), G190 (= G185), G191 (= G186), L192 (= L187), S211 (= S206), T212 (= T207), S213 (= S208), K216 (= K210), T251 (= T245), V252 (= V246), S253 (= S247), A254 (= A248), V274 (= V268), G275 (= G269), A276 (≠ V270), A299 (≠ G293), I300 (= I294), R345 (= R339)
- binding zinc ion: C47 (= C41), H69 (= H63), C100 (= C94), C103 (= C97), C106 (= C100), C114 (= C108), C163 (= C159)
7cguA Crystal structure of abhar
51% identity, 98% coverage: 4:344/347 of query aligns to 4:345/352 of 7cguA
3twoB The crystal structure of cad from helicobacter pylori complexed with NADP(h) (see paper)
47% identity, 99% coverage: 2:344/347 of query aligns to 3:343/348 of 3twoB
- active site: C42 (= C41), H43 (= H42), S44 (= S43), H47 (= H46), H64 (= H63), E65 (= E64), C95 (= C94), C98 (= C97), C101 (= C100), C109 (= C108), V113 (≠ T113), C160 (= C159), T164 (= T163), R338 (= R339)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: C42 (= C41), H43 (= H42), S44 (= S43), H47 (= H46), C160 (= C159), T164 (= T163), G184 (= G183), F185 (≠ L184), G186 (= G185), G187 (= G186), L188 (= L187), R208 (≠ T207), T241 (= T245), I242 (≠ V246), P243 (≠ S247), T244 (≠ A248), V264 (= V268), G265 (= G269), L266 (≠ V270), L292 (≠ G293), I293 (= I294), L330 (≠ M331), G333 (= G334), R338 (= R339)
- binding zinc ion: C42 (= C41), H64 (= H63), C95 (= C94), C98 (= C97), C101 (= C100), C109 (= C108), C160 (= C159)
5vktA Cinnamyl alcohol dehydrogenases (sbcad4) from sorghum bicolor (l.) Moench (see paper)
49% identity, 98% coverage: 6:345/347 of query aligns to 5:346/353 of 5vktA
- active site: C40 (= C41), H41 (= H42), T42 (≠ S43), H45 (= H46), H62 (= H63), E63 (= E64), C93 (= C94), C96 (= C97), C99 (= C100), C107 (= C108), V111 (≠ T113), C158 (= C159), T162 (= T163), R340 (= R339)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: C40 (= C41), H41 (= H42), T42 (≠ S43), H45 (= H46), C158 (= C159), T162 (= T163), G183 (= G183), L184 (= L184), G185 (= G185), G186 (= G186), L187 (= L187), S206 (= S206), S207 (≠ T207), S208 (= S208), K211 (= K210), T246 (= T245), V247 (= V246), S248 (= S247), A249 (= A248), V269 (= V268), G270 (= G269), N293 (≠ S292), G294 (= G293), N335 (≠ G334), R340 (= R339)
- binding zinc ion: C40 (= C41), H62 (= H63), C93 (= C94), C96 (= C97), C99 (= C100), C107 (= C108), C158 (= C159)
5fi3A Heteroyohimbine synthase thas1 from catharanthus roseus - complex with NADP+ (see paper)
44% identity, 98% coverage: 6:344/347 of query aligns to 8:338/344 of 5fi3A
- active site: C43 (= C41), Q44 (≠ H42), Y45 (≠ S43), E48 (≠ H46), H65 (= H63), E66 (= E64), C96 (= C94), C99 (= C97), C102 (= C100), C110 (= C108), G114 (≠ N112), C151 (= C159), R333 (= R339)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: Q44 (≠ H42), E48 (≠ H46), T155 (= T163), G176 (= G183), L177 (= L184), G178 (= G185), G179 (= G186), L180 (= L187), S199 (= S206), S200 (≠ T207), K204 (= K211), T239 (= T245), I240 (≠ V246), P241 (≠ S247), L262 (≠ V268), G263 (= G269), T287 (≠ G293), S328 (≠ G334)
- binding zinc ion: C43 (= C41), H65 (= H63), E66 (= E64), C96 (= C94), C99 (= C97), C102 (= C100), C110 (= C108), C151 (= C159)
8b1vA Dihydroprecondylocarpine acetate synthase 2 from tabernanthe iboga (see paper)
47% identity, 98% coverage: 6:344/347 of query aligns to 12:351/358 of 8b1vA
8b25B Dihydroprecondylocarpine acetate synthase 2 from tabernanthe iboga - stemmadenine acetate bound structure (see paper)
47% identity, 98% coverage: 6:344/347 of query aligns to 12:351/359 of 8b25B
8a3nB Geissoschizine synthase from catharanthus roseus - binary complex with NADP+ (see paper)
44% identity, 98% coverage: 6:346/347 of query aligns to 9:347/352 of 8a3nB
- binding nadp nicotinamide-adenine-dinucleotide phosphate: N45 (≠ H42), V161 (≠ T163), G182 (= G183), L183 (= L184), L186 (= L187), S205 (= S206), T206 (= T207), K210 (= K211), T245 (= T245), P247 (vs. gap), V269 (= V268), A271 (≠ V270), S294 (≠ G293), L335 (≠ G334), R340 (= R339)
- binding zinc ion: C44 (= C41), H66 (= H63), E67 (= E64), C97 (= C94), C100 (= C97), C103 (= C100), C111 (= C108), C157 (= C159)
5h81B Heteroyohimbine synthase thas2 from catharanthus roseus - complex with NADP+ (see paper)
43% identity, 98% coverage: 6:344/347 of query aligns to 6:348/354 of 5h81B
- active site: A343 (≠ R339)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V174 (≠ T163), F196 (≠ L184), G197 (= G185), R198 (≠ G186), I199 (≠ L187), S218 (= S206), T219 (= T207), S220 (= S208), K223 (= K210), V259 (= V246), P260 (≠ S247), L281 (≠ V268), I297 (≠ G293)
- binding zinc ion: C41 (= C41), H63 (= H63), E64 (= E64), C94 (= C94), C97 (= C97), C100 (= C100), C108 (= C108), C170 (= C159)
5h83A Heteroyohimbine synthase hys from catharanthus roseus - apo form (see paper)
42% identity, 99% coverage: 2:346/347 of query aligns to 9:349/354 of 5h83A
O49482 Cinnamyl alcohol dehydrogenase 5; AtCAD5; Cinnamyl alcohol dehydrogenase D; EC 1.1.1.195 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
45% identity, 98% coverage: 4:344/347 of query aligns to 10:350/357 of O49482
- C47 (= C41) binding
- T49 (≠ S43) binding
- H69 (= H63) binding
- E70 (= E64) binding ; mutation to A: Loss of activity.
- C100 (= C94) binding
- C103 (= C97) binding
- C106 (= C100) binding
- C114 (= C108) binding
- C163 (= C159) binding
- T167 (= T163) binding
- GLGGVG 188:193 (≠ GLGGLG 183:188) binding
- SSSNKK 211:216 (≠ STSPKK 206:211) binding
- T251 (= T245) binding
- G275 (= G269) binding
- SFI 298:300 (≠ SGI 292:294) binding
2cf6A Crystal structures of the arabidopsis cinnamyl alcohol dehydrogenases atcad5 (see paper)
45% identity, 98% coverage: 4:344/347 of query aligns to 5:345/352 of 2cf6A
- active site: C42 (= C41), H43 (= H42), T44 (≠ S43), H47 (= H46), H64 (= H63), E65 (= E64), C95 (= C94), C98 (= C97), C101 (= C100), C109 (= C108), I113 (≠ T113), C158 (= C159), T162 (= T163), R340 (= R339)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: H43 (= H42), T44 (≠ S43), T162 (= T163), L184 (= L184), G185 (= G185), G186 (= G186), V187 (≠ L187), S206 (= S206), S207 (≠ T207), K211 (= K211), T246 (= T245), M269 (≠ V268), S293 (= S292), F294 (≠ G293), I295 (= I294)
- binding zinc ion: C42 (= C41), H64 (= H63), E65 (= E64), C98 (= C97), C109 (= C108)
2cf5A Crystal structures of the arabidopsis cinnamyl alcohol dehydrogenases, atcad5 (see paper)
45% identity, 98% coverage: 4:344/347 of query aligns to 5:345/352 of 2cf5A
- active site: C42 (= C41), H43 (= H42), T44 (≠ S43), H47 (= H46), H64 (= H63), E65 (= E64), C95 (= C94), C98 (= C97), C101 (= C100), C109 (= C108), I113 (≠ T113), C158 (= C159), T162 (= T163), R340 (= R339)
- binding zinc ion: C42 (= C41), H64 (= H63), E65 (= E64), C95 (= C94), C98 (= C97), C101 (= C100), C109 (= C108), C158 (= C159)
Q6ZHS4 Cinnamyl alcohol dehydrogenase 2; OsCAD2; Protein GOLD HULL AND INTERNODE 2; EC 1.1.1.195 from Oryza sativa subsp. japonica (Rice) (see paper)
44% identity, 98% coverage: 6:345/347 of query aligns to 12:351/363 of Q6ZHS4
- G185 (≠ A180) mutation to D: Loss of activity.
Query Sequence
>CA265_RS12755 FitnessBrowser__Pedo557:CA265_RS12755
MIATKGYAAQNAETDLAPWNFERREVGPHDVQFEILFCGVCHSDLHQIKNDWFPGIFPMV
PGHEIVGRVVKVGDHVKKFKVGDLAGTGCMVDSCQVCENCKQNLEQYCLEGNTQTYNGLE
RDGKTPTYGGYSDSIVVREEFVLHVSDKLNLAAVAPLLCAGITTYSPLKHWKVGKGHKLA
VLGLGGLGHMAVKFGVAFGAEVTVLSTSPKKEEDAKKLGAHHFVVTTDPAQVKAARGTFD
FILDTVSAEHDFNMYLSLLRTNGIHICVGVPPKPAEIAAFSLLGGRKSLAGSGIGGIAET
QEMLDFCAENNIVSDIEMIDIKDIHHAYERMEKGDVRYRFVIDMATL
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory