SitesBLAST
Comparing CA265_RS13665 CA265_RS13665 galactonate dehydratase to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
2o56A Crystal structure of a member of the enolase superfamily from salmonella typhimurium
32% identity, 98% coverage: 1:379/388 of query aligns to 2:389/392 of 2o56A
- active site: K165 (= K152), D167 (= D154), M171 (≠ H158), L186 (= L173), E214 (= E201), H216 (= H203), E240 (= E227), G265 (= G254), E266 (= E255), Q287 (≠ M276), D289 (= D278), H316 (= H305), E341 (= E330), A346 (≠ V336)
- binding magnesium ion: E214 (= E201), E240 (= E227), E266 (= E255)
2qq6B Crystal structure of mandelate racemase/muconate lactonizing enzyme- like protein from rubrobacter xylanophilus dsm 9941
32% identity, 97% coverage: 2:376/388 of query aligns to 4:386/396 of 2qq6B
- active site: P37 (= P39), G79 (= G78), D124 (≠ N123), K166 (= K152), D168 (= D154), D213 (≠ E201), H215 (= H203), E239 (= E227), G264 (= G254), E265 (= E255), M286 (= M276), D288 (= D278), H315 (= H305), N316 (= N306), E340 (= E330), D345 (= D335)
- binding magnesium ion: D213 (≠ E201), E239 (= E227), E265 (= E255), H315 (= H305)
2ox4C Crystal structure of putative dehydratase from zymomonas mobilis zm4
30% identity, 96% coverage: 1:371/388 of query aligns to 2:381/397 of 2ox4C
- active site: K165 (= K152), D167 (= D154), E214 (= E201), H216 (= H203), E240 (= E227), G265 (= G254), E266 (= E255), Q287 (≠ M276), D289 (= D278), H316 (= H305), E341 (≠ Q329)
- binding magnesium ion: E214 (= E201), E240 (= E227), E266 (= E255)
4e6mA Crystal structure of putative dehydratase protein from salmonella enterica subsp. Enterica serovar typhimurium (salmonella typhimurium)
30% identity, 97% coverage: 1:376/388 of query aligns to 2:395/401 of 4e6mA
- active site: L45 (vs. gap), G48 (≠ W38), K169 (= K152), D171 (= D154), I175 (vs. gap), E223 (= E201), H225 (= H203), E249 (= E227), G274 (= G254), E275 (= E255), R276 (= R256), Q296 (≠ M276), D298 (= D278), H325 (= H305), C327 (≠ P307), E350 (= E330), A355 (≠ D335)
- binding magnesium ion: E223 (= E201), E249 (= E227), E275 (= E255)
3sjnA Crystal structure of enolase spea_3858 (target efi-500646) from shewanella pealeana with magnesium bound
30% identity, 97% coverage: 1:376/388 of query aligns to 2:370/373 of 3sjnA
- active site: S46 (≠ N37), L49 (≠ I43), T139 (≠ N123), K165 (= K152), G167 (≠ T159), M171 (≠ Y163), D198 (≠ E201), A200 (≠ H203), E225 (= E227), I247 (= I251), G250 (= G254), E251 (= E255), S252 (≠ R256), Q272 (≠ M276), D274 (= D278), H301 (= H305), G302 (≠ N306), F303 (≠ P307), M325 (≠ Q329), E326 (= E330), Q329 (≠ F333), S331 (≠ D335)
- binding magnesium ion: D198 (≠ E201), E225 (= E227), E251 (= E255)
5olcC Crystal structure of the 3,6-anhydro-d-galactonate cycloisomerase from zobellia galactanivorans (see paper)
29% identity, 92% coverage: 20:376/388 of query aligns to 23:348/351 of 5olcC
- active site: K148 (= K152), K150 (≠ S168), D178 (≠ E201), N180 (≠ H203), E204 (= E227), G229 (= G254), E230 (= E255), D253 (= D278), H280 (= H305), E304 (= E330), E309 (≠ D335)
- binding magnesium ion: D178 (≠ E201), E204 (= E227), E230 (= E255)
4hpnA Crystal structure of a proposed galactarolactone cycloisomerase from agrobacterium tumefaciens, target efi-500704, with bound ca, ordered loops (see paper)
29% identity, 95% coverage: 1:368/388 of query aligns to 1:365/378 of 4hpnA
- active site: F19 (vs. gap), G50 (≠ N37), R53 (≠ S41), T134 (≠ N123), K164 (= K152), K166 (≠ H158), D194 (≠ E201), N196 (≠ H203), E220 (= E227), G245 (= G254), E246 (= E255), T247 (≠ R256), Q267 (≠ M276), D269 (= D278), H296 (= H305), V297 (≠ N306), W298 (≠ P307), R320 (= R328), E329 (= E330), F330 (= F331), H334 (≠ D335)
- binding calcium ion: D194 (≠ E201), D209 (≠ Q216), E220 (= E227), G237 (≠ R244), E246 (= E255)
3rraB Crystal structure of enolase prk14017 (target efi-500653) from ralstonia pickettii 12j with magnesium bound
31% identity, 91% coverage: 17:370/388 of query aligns to 15:349/379 of 3rraB
- active site: I35 (≠ N37), R38 (≠ G40), Y118 (= Y121), K145 (= K152), N147 (≠ D154), E151 (≠ H158), D184 (≠ E201), H186 (= H203), E210 (= E227), G235 (= G254), E236 (= E255), R237 (= R256), Q257 (≠ M276), D259 (= D278), H286 (= H305), P288 (= P307), E311 (≠ D340)
- binding magnesium ion: D184 (≠ E201), E210 (= E227), E236 (= E255)
4e4fB Crystal structure of enolase pc1_0802 (target efi-502240) from pectobacterium carotovorum subsp. Carotovorum pc1
29% identity, 97% coverage: 1:377/388 of query aligns to 2:362/381 of 4e4fB
- active site: L37 (vs. gap), R40 (≠ P39), R148 (≠ K152), Q150 (≠ D154), D189 (≠ E201), H191 (= H203), E215 (= E227), G240 (= G254), E241 (= E255), V242 (≠ R256), R262 (≠ M276), T264 (≠ D278), H291 (= H305), P293 (= P307), E318 (= E330)
- binding magnesium ion: D189 (≠ E201), E215 (= E227), E241 (= E255)
Sites not aligning to the query:
C6D9S0 D-galactonate dehydratase family member PC1_0802; D-mannonate dehydratase; EC 4.2.1.-; EC 4.2.1.8 from Pectobacterium carotovorum subsp. carotovorum (strain PC1) (see paper)
29% identity, 97% coverage: 1:377/388 of query aligns to 1:385/404 of C6D9S0
- D212 (≠ E201) binding
- E238 (= E227) binding
- E264 (= E255) binding
3rcyF Crystal structure of mandelate racemase/muconate lactonizing enzyme- like protein from roseovarius sp. Tm1035
29% identity, 94% coverage: 13:377/388 of query aligns to 19:378/397 of 3rcyF
- active site: K165 (= K152), D167 (= D154), R175 (≠ Y163), G208 (≠ E201), H210 (= H203), E234 (= E227), G259 (= G254), E260 (= E255), Q281 (≠ M276), A283 (≠ D278), H310 (= H305), A313 (≠ N308), L332 (≠ F327), E335 (= E330)
- binding magnesium ion: E234 (= E227), E260 (= E255)
- binding alpha-D-ribofuranose: R85 (≠ G78), P86 (= P79), P239 (= P232), D240 (≠ E233), L262 (≠ H257), A266 (≠ H261), E267 (≠ G262), E267 (≠ G262)
3ekgA Crystal structure of l-rhamnonate dehydratase from azotobacter vinelandii complexed with mg and l-tartrate
30% identity, 88% coverage: 32:371/388 of query aligns to 71:386/395 of 3ekgA