SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing CA265_RS14660 FitnessBrowser__Pedo557:CA265_RS14660 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

6pnzA The structure of the aspartate transcarbamoylase trimer from staphylococcus aureus complexed with pala at 2.27 resolution.
40% identity, 94% coverage: 12:304/311 of query aligns to 3:288/293 of 6pnzA

query
sites
6pnzA

H

H

L

L

G

S

I

M

K

E

D

H

I

L

N

S

R

T

N

D

D

Q

I

I

E

Y

L

K

I

L

F

I

E

Q

T

K

A

A

D

S

N

Q

F

F

K

K

E

S

V

-

I

-

N

-

R

-

P

G

I

E

K

R

K

Q

V

L

P

P

S

N

L

F

R

E

D

G

I

K

T

Y

I

V

A

A

N

N

I

L

F

F

E

E

N

N

S
|
S

T
|
T

R
|
R

T
|
T

K

K

L

C

S

S

F

F

E

E

L

M

A

A

E

E

K

L

R

K

L

L

S

G

A

L

D

K

V

T

V

I

N

S

F

F

A

E

A

T

S

S

S

T

S

S

S
|
S

V

V

S

S

K
|
K

G

G

E

E

T

S

L

L

I

Y

D

D

T

T

V

C

N

K

N

T

I

L

L

E

S

S

M

I

K

G

V

C

D

D

M

L

V

L

V

V

M

I

R
|
R

H

H

P

P

Y

F

A

N

G

N

A

Y

G

Y

Q

E

F

K

L

L

S

A

K

-

H

N

V

I

K

N

A

I

Q

P

I

I

V

A

N

N

A

A

G

G

D

D

G

G

A

S

H

G

E

Q

H
|
H

P

P

T

T

Q

Q

A

S

L

L

D

D

A

L

F

M

S

T

I

I

R

Y

Q

E

K

E

L

Y

G

G

D

Y

V

F

A

E

G

G

K

L

K

N

V

V

V

L

I

I

V

C

G

G

D

D

I

I

L

K

H

N

S

S

R
|
R

V

V

A

A

I

R

S

S

N

N

I

Y

L

H

C

S

L

L

Q

K

R

A

L

L

G

G

A

A

E

N

V

V

M

M

V

F

C

N

G

S

P

P

T

N

T

A

L

W

I

I

P

D

K

D

H

S

I

L

H

E

Q

A

L

P

G

Y

V

V

K

-

V

-

E

-

H

-

N

N

L

I

I

D

K

D

A

V

L

I

N

E

W

T

C

V

D

D

V

I

A

V

N

M

M

L

L

L

R
|
R

I

I

Q
|
Q

L

H

E

E

R

R

Q

H

D

G

I

L

K

A

Y

E

F

E

P

T

S

R

L

F

R

A

-

A

-

D

E

D

Y

Y

A

H

M

Q

M

K

Y

H

G

G

L

L

N

N

K

E

Q

V

I

R

L

Y

D

N

N

K

L

L

D

Q

K

E

E

H

I

A

I

I

V

V

M

M

H

H

P

P

G
x
A

P

P

I

V

N

N

R

R

G

G

V

V

E

E

I

I

T

Q

S

S

D

D

V

L

A

V

D

E

S

A

K

S

Q

K

S

S

I

R

I

I

L

F

E

K

Q

Q

V

M

E

E

N

N

G

G

V

V

A

Y

V

L

R

R

M

M

A

A

V

V

L

I

binding n-(phosphonacetyl)-l-aspartic acid: S48 (= S61), T49 (= T62), R50 (= R63), T51 (= T64), S75 (= S88), K78 (= K91), R100 (= R113), H127 (= H141), R160 (= R174), R210 (= R228), Q212 (= Q230), A253 (≠ G269)

4bjhB Crystal structure of the aquifex reactor complex formed by dihydroorotase (h180a, h232a) with dihydroorotate and aspartate transcarbamoylase with n-(phosphonacetyl)-l-aspartate (pala) (see paper)
39% identity, 96% coverage: 11:309/311 of query aligns to 2:291/291 of 4bjhB

query
sites
4bjhB

R

R

H

S

L

L

L

I

G

S

I

S

K

L

D

D

I

L

N

T

R

R

N

E

D

E

I

V

E

E

L

E

I

I

F

L

E

K

T

Y

A

A

D

K

N

E

F

F

K

K

E

E

V

G

I

K

N

E

R

E

P

T

I

I

K

K

K

A

V

S

P

A

S

V

L

L

R

-

D

-

I

-

T

-

I

-

A

-

N

-

I

F

F

F

F

S

E

E

N

P

S
|
S

T
|
T

R
|
R

T
|
T

K

R

L

L

S

S

F

F

E

E

L

K

A

A

E

A

K

R

R

E

L

L

S

G

A

I

D

E

V

T

V

Y

N

L

F

V

A

S

A

G

S

S

S

E

S

S

V

T

S

V

K
|
K

G

G

E

E

T

S

L

F

I

F

D

D

T

T

V

L

N

K

N

T

I

F

L

E

S

G

M

L

K

G

V

F

D

D

M

Y

V

V

M

F

R
|
R

H

V

P

P

Y

F

A

V

G

F

A

F

G

P

-

Y

Q

K

F

E

L

I

S

V

K

K

H

S

V

L

K

N

A

L

Q

R

I

L

V

V

N

N

A

A

G

G

D

D

G

G

A

T

H

H

E

Q

H
|
H

P

P

T

S

Q
|
Q

A

G

L

L

L

I

D

D

A

F

F

F

S

T

I

I

R

K

Q

E

K

H

L

F

G

G

D

E

V

V

A

K

G

D

K

L

K

R

V

V

V

L

I

Y

V

V

G

G

D

D

I

I

L

K

H

H

S

S

R
|
R

V
|
V

A

F

I

R

S

S

N

G

I

A

L

P

C

L

L

L

Q

N

R

M

L

F

G

G

A

A

E

K

V

I

M

G

V

V

C

C

G

G

P

P

T

K

T

T

L

L

I

I

P

P

K

R

H

D

I

V

H

E

Q

V

L

F

G

K

V

V

K

D

V

V

E

F

H

D

N

D

L

V

I

D

K

K

A

G

L

I

N

D

W

W

C

A

D

D

V

V

A

V

N

I

M

W

L

L

R
|
R

I

L

Q
|
Q

L

K

E

E

R

R

Q

Q

D

K

I

E

K

N

Y

Y

F

I

P

P

S

S

L

E

R

S

E

S

Y

Y

A

F

M

K

M

Q

Y

F

G

G

L

L

N

T

K

K

Q

E

I

-

L

-

D

-

N

R

L

F

D

E

K

K

E

V

I

K

I

L

V

Y

M

M

H

H

P

P

G
|
G

P

P

I

V

N

N

R

R

G

N

V

V

E

D

I

I

T

D

S

H

D

E

V

L

A

V

D

Y

S

T

K

E

Q

K

S

S

I

L

I

I

L

Q

E

E

Q

Q

V

V

E

K

N

N

G

G

V

I

A

P

V

V

R

R

M

K

A

A

V

I

L

Y

Y

K

L

F

L

L

A

W

S

T

active site: R47 (= R63), T48 (= T64), K75 (= K91), R97 (= R113), H126 (= H141), Q129 (= Q144)
binding n-(phosphonacetyl)-l-aspartic acid: S45 (= S61), T46 (= T62), R47 (= R63), T48 (= T64), R97 (= R113), H126 (= H141), R159 (= R174), V160 (= V175), R213 (= R228), Q215 (= Q230), G251 (= G269)

3d6nB Crystal structure of aquifex dihydroorotase activated by aspartate transcarbamoylase (see paper)
39% identity, 96% coverage: 11:309/311 of query aligns to 2:291/291 of 3d6nB

query
sites
3d6nB

R

R

H

S

L

L

L

I

G

S

I

S

K

L

D

D

I

L

N

T

R

R

N

E

D

E

I

V

E

E

L

E

I

I

F

L

E

K

T

Y

A

A

D

K

N

E

F

F

K

K

E

E

V

G

I

K

N

E

R

E

P

T

I

I

K

K

K

A

V

S

P

A

S

V

L

L

R

-

D

-

I

-

T

-

I

-

A

-

N

-

I

F

F

F

F

S

E

E

N

P

S

S

T

T

R
|
R

T
|
T

K

R

L

L

S

S

F

F

E

E

L

K

A

A

E

A

K

R

R

E

L

L

S

G

A

I

D

E

V

T

V

Y

N

L

F

V

A

S

A

G

S

S

S

E

S

S

V

T

S

V

K
|
K

G

G

E

E

T

S

L

F

I

F

D

D

T

T

V

L

N

K

N

T

I

F

L

E

S

G

M

L

K

G

V

F

D

D

M

Y

V

V

M

F

R
|
R

H

V

P

P

Y

F

A

V

G

F

A

F

G

P

-

Y

Q

K

F

E

L

I

S

V

K

K

H

S

V

L

K

N

A

L

Q

R

I

L

V

V

N

N

A

A

G

G

D

D

G

G

A

T

H

H

E

Q

H
|
H

P

P

T

S

Q
|
Q

A

G

L

L

L

I

D

D

A

F

F

F

S

T

I

I

R

K

Q

E

K

H

L

F

G

G

D

E

V

V

A

K

G

D

K

L

K

R

V

V

V

L

I

Y

V

V

G

G

D

D

I

I

L

K

H

H

S

S

R
|
R

V
|
V

A

F

I

R

S

S

N

G

I

A

L

P

C

L

L

L

Q

N

R

M

L

F

G

G

A

A

E

K

V

I

M

G

V

V

C

C

G

G

P

P

T

K

T

T

L

L

I

I

P

P

K

R

H

D

I

V

H

E

Q

V

L

F

G

K

V

V

K

D

V

V

E

F

H

D

N

D

L

V

I

D

K

K

A

G

L

I

N

D

W

W

C

A

D

D

V

V

A

V

N

I

M

W

L

L

R
|
R

I

L

Q

Q

L

K

E

E

R

R

Q

Q

D

K

I

E

K

N

Y

Y

F

I

P

P

S

S

L

E

R

S

E

S

Y

Y

A

F

M

K

M

Q

Y

F

G

G

L

L

N

T

K

K

Q

E

I

-

L

-

D

-

N

R

L

F

D

E

K

K

E

V

I

K

I

L

V

Y

M

M

H

H

P

P

G
|
G

P

P

I

V

N

N

R

R

G

N

V

V

E

D

I

I

T

D

S

H

D

E

V

L

A

V

D

Y

S

T

K

E

Q

K

S

S

I

L

I

I

L

Q

E

E

Q

Q

V

V

E

K

N

N

G

G

V

I

A

P

V

V

R

R

M

K

A

A

V

I

L

Y

Y

K

L

F

L

L

A

W

S

T

active site: R47 (= R63), T48 (= T64), K75 (= K91), R97 (= R113), H126 (= H141), Q129 (= Q144)
binding citrate anion: T48 (= T64), R97 (= R113), H126 (= H141), R159 (= R174), V160 (= V175), R213 (= R228), G251 (= G269)

3r7lA Crystal structure of pala-bound aspartate transcarbamoylase from bacillus subtilis (see paper)
40% identity, 95% coverage: 11:304/311 of query aligns to 2:285/290 of 3r7lA

query
sites
3r7lA

R

K

H

H

L

L

L

T

G

T

I

M

K

S

D

E

I

L

N

S

R

T

N

E

D

E

I

I

E

K

L

D

I

L

F

L

E

Q

T

T

A

A

D

Q

N

E

F

L

K

K

E

-

V

-

I

-

N

-

R

-

P

S

I

G

K

K

K

T

V

D

P

N

S

Q

L

L

R

T

D

G

I

K

T

F

I

A

A

A

N

N

I

L

F

F

E

E

N

P

S
|
S

T
|
T

R
|
R

T
|
T

K

R

L

F

S

S

F

F

E

E

L

V

A

A

E

E

K

K

R

K

L

L

S

G

A

M

D

N

V

V

V

L

N

N

F

L

A

D

A

G

S

T

S

S

S

T

S
|
S

V

V

S

Q

K
|
K

G

G

E

E

T

T

L

L

I

Y

D

D

T

T

V

I

N

R

N

T

I

L

L

E

S

S

M

I

K

G

V

V

D

D

M

V

V

C

V

V

M

I

R
|
R

H

H

P

S

Y

E

A

D

G

E

A

Y

G

Y

Q

E

F

E

L

L

S

V

K

S

H

Q

V

V

K

N

A

I

Q

P

I

I

V

L

N

N

A

A

G

G

D

D

G

G

A

C

H

G

E

Q

H
|
H

P

P

T

T

Q
|
Q

A

S

L

L

D

D

A

L

F

M

S

T

I

I

R

Y

Q

E

K

E

L

F

G

N

D

T

V

F

A

K

G

G

K

L

K

T

V

V

V

S

I

I

V

H

G

G

D

D

I

I

L

K

H

H

S

S

R
|
R

V

V

A

A

I

R

S

S

N

N

I

A

L

E

C

V

L

L

Q

T

R

R

L

L

G

G

A

A

E

R

V

V

M

L

V

F

C

S

G

G

P

P

T

S

T

E

L

W

I

Q

P

D

K

E

H

E

I

-

H

N

Q

T

L

F

G

G

V

T

K

Y

V

V

E

-

H

-

N

S

L

M

I

D

K

E

A

A

L

V

N

E

W

S

C

S

D

D

V

V

A

V

N

M

M

L

L
|
L

R
|
R

I

I

Q
|
Q

L

N

E

E

R

R

Q

H

D

-

I

-

K

Q

Y

S

F

A

P

V

S

S

L

Q

R

E

E

G

Y

Y

A

L

M

N

M

K

Y

Y

G

G

L

L

N

T

K

V

Q

E

I

R

L

A

D

E

N

R

L

M

D

K

K

R

E

H

I

A

I

I

V

I

M

M

H

H

P
|
P

G
x
A

P

P

I

V

N

N

R

R

G

G

V

V

E

E

I

I

T

D

S

D

D

S

V

L

A

V

D

E

S

S

K

E

Q

K

S

S

I

R

I

I

L

F

E

K

Q

Q

V

M

E

K

N

N

G
|
G

V

V

A

F

V

I

R

R

M

M

A

A

V

V

L

I

active site: R49 (= R63), T50 (= T64), K77 (= K91), R99 (= R113), H127 (= H141), Q130 (= Q144), L210 (= L227), P249 (= P268), G277 (= G296)
binding n-(phosphonacetyl)-l-aspartic acid: S47 (= S61), T48 (= T62), R49 (= R63), T50 (= T64), S74 (= S88), K77 (= K91), R99 (= R113), H127 (= H141), R160 (= R174), R211 (= R228), Q213 (= Q230), A250 (≠ G269)

P05654 Aspartate carbamoyltransferase catalytic subunit; Aspartate transcarbamylase; ATCase; EC 2.1.3.2 from Bacillus subtilis (strain 168) (see paper)
40% identity, 95% coverage: 11:304/311 of query aligns to 2:285/304 of P05654

query
sites
P05654

R

K

H

H

L

L

L

T

G

T

I

M

K

S

D

E

I

L

N

S

R

T

N

E

D

E

I

I

E

K

L

D

I

L

F

L

E

Q

T

T

A

A

D

Q

N

E

F

L

K

K

E

-

V

-

I

-

N

-

R

-

P

S

I

G

K

K

K

T

V

D

P

N

S

Q

L

L

R

T

D

G

I

K

T

F

I

A

A

A

N

N

I

L

F

F

E

E

N

P

S

S

T

T

R

R

T

T

K

R

L

F

S

S

F

F

E

E

L

V

A

A

E

E

K

K

R

K

L

L

S

G

A

M

D

N

V

V

V

L

N

N

F

L

A

D

A

G

S

T

S

S

S

T

S

S

V

V

S

Q

K

K

G

G

E

E

T

T

L

L

I

Y

D

D

T

T

V

I

N

R

N

T

I

L

L

E

S

S

M

I

K

G

V

V

D

D

M

V

V

C

V

V

M

I

R

R

H

H

P

S

Y

E

A

D

G

E

A

Y

G

Y

Q

E

F

E

L

L

S

V

K

S

H

Q

V

V

K

N

A

I

Q

P

I

I

V

L

N

N

A

A

G

G

D

D

G

G

A

C

H

G

E

Q

H

H

P

P

T

T

Q

Q

A

S

L

L

D

D

A

L

F

M

S

T

I

I

R

Y

Q

E

K

E

L

F

G

N

D

T

V

F

A

K

G

G

K

L

K

T

V

V

V

S

I

I

V

H

G

G

D

D

I

I

L

K

H

H

S

S

R

R

V

V

A

A

I

R

S

S

N

N

I

A

L

E

C

V

L

L

Q

T

R

R

L

L

G

G

A

A

E

R

V

V

M

L

V

F

C

S

G

G

P

P

T

S

T

E

L

W

I

Q

P

D

K

E

H

E

I

-

H

N

Q

T

L

F

G

G

V

T

K

Y

V

V

E

-

H

-

N

S

L

M

I

D

K

E

A

A

L

V

N

E

W

S

C

S

D

D

V

V

A

V

N

M

M

L

L

L

R

R

I

I

Q

Q

L

N

E

E

R

R

Q

H

D

-

I

-

K

Q

Y

S

F

A

P

V

S

S

L

Q

R

E

E

G

Y

Y

A

L

M

N

M

K

Y

Y

G

G

L

L

N

T

K

V

Q

E

I

R

L

A

D

E

N

R

L

M

D

K

K

R

E

H

I

A

I

I

V

I

M

M

H

H

P

P

G

A

P

P

I

V

N

N

R

R

G

G

V

V

E

E

I

I

T

D

S

D

D

S

V

L

A

V

D

E

S

S

K

E

Q

K

S

S

I

R

I

I

L

F

E

K

Q

Q

V

M

E

K

N

N

G

G

V

V

A

F

V

I

R

R

M

M

A

A

V

V

L

I

Sites not aligning to the query:

303 modified: Phosphoserine

3r7fA Crystal structure of cp-bound aspartate transcarbamoylase from bacillus subtilis (see paper)
40% identity, 95% coverage: 11:304/311 of query aligns to 2:285/291 of 3r7fA

query
sites
3r7fA

R

K

H

H

L

L

L

T

G

T

I

M

K

S

D

E

I

L

N
x
S

R
x
T

N

E

D

E

I

I

E

K

L

D

I

L

F

L

E

Q

T

T

A

A

D
x
Q

N

E

F

L

K
|
K

E

-

V

-

I

-

N

-

R

-

P

S

I

G

K

K

K

T

V

D

P

N

S

Q

L

L

R

T

D

G

I

K

T

F

I

A

A

A

N

N

I

L

F

F

E

E

N

P

S
|
S

T
|
T

R
|
R

T
|
T

K

R

L

F

S

S

F

F

E

E

L

V

A

A

E

E

K

K

R

K

L

L

S

G

A

M

D

N

V

V

V

L

N

N

F

L

A

D

A

G

S

T

S

S

S

T

S

S

V

V

S

Q

K
|
K

G

G

E

E

T

T

L

L

I

Y

D

D

T

T

V

I

N

R

N

T

I

L

L

E

S

S

M

I

K

G

V

V

D

D

M

V

V

C

V

V

M

I

R
|
R

H

H

P

S

Y

E

A

D

G

E

A

Y

G

Y

Q

E

F

E

L

L

S

V

K

S

H

Q

V

V

K

N

A

I

Q

P

I

I

V

L

N

N

A

A

G

G

D

D

G

G

A

C

H

G

E

Q

H
|
H

P

P

T

T

Q
|
Q

A

S

L

L

D

D

A

L

F

M

S

T

I

I

R

Y

Q
x
E

K

E

L

F

G

N

D

T

V

F

A

K

G

G

K

L

K

T

V

V

V

S

I

I

V

H

G

G

D

D

I

I

L

K

H

H

S

S

R

R

V

V

A

A

I

R

S

S

N

N

I

A

L

E

C

V

L

L

Q

T

R
|
R

L

L

G

G

A

A

E

R

V

V

M

L

V

F

C

S

G

G

P

P

T

S

T

E

L

W

I

Q

P

D

K

E

H

E

I

-

H

N

Q

T

L

F

G

G

V

T

K

Y

V

V

E

-

H

-

N

S

L

M

I

D

K

E

A

A

L

V

N

E

W

S

C

S

D

D

V

V

A

V

N

M

M

L

L
|
L

R

R

I

I

Q

Q

L

N

E

E

R

R

Q

H

D

-

I

-

K

Q

Y

S

F

A

P

V

S

S

L

Q

R

E

E

G

Y

Y

A

L

M

N

M

K

Y

Y

G

G

L

L

N

T

K

V

Q

E

I

R

L

A

D

E

N

R

L

M

D
x
K

K

R

E
x
H

I

A

I

I

V

I

M

M

H

H

P
|
P

G
x
A

P

P

I

V

N

N

R

R

G

G

V

V

E

E

I

I

T

D

S

D

D

S

V

L

A

V

D

E

S

S

K

E

Q

K

S

S

I

R

I

I

L

F

E
x
K

Q

Q

V

M

E
x
K

N

N

G
|
G

V

V

A

F

V

I

R

R

M

M

A

A

V

V

L

I

active site: R49 (= R63), T50 (= T64), K77 (= K91), R99 (= R113), H127 (= H141), Q130 (= Q144), L210 (= L227), P249 (= P268), G277 (= G296)
binding phosphoric acid mono(formamide)ester: S47 (= S61), T48 (= T62), R49 (= R63), T50 (= T64), R99 (= R113), H127 (= H141), Q130 (= Q144), P249 (= P268), A250 (≠ G269)
binding phosphate ion: S11 (≠ N20), T12 (≠ R21), Q23 (≠ D32), K26 (= K35), E140 (≠ Q154), R171 (= R185), K241 (≠ D260), H243 (≠ E262), K272 (≠ E291), K272 (≠ E291), K275 (≠ E294)

3r7dA Crystal structure of unliganded aspartate transcarbamoylase from bacillus subtilis (see paper)
40% identity, 95% coverage: 11:304/311 of query aligns to 2:285/291 of 3r7dA

query
sites
3r7dA

R

K

H

H

L

L

L

T

G

T

I

M

K

S

D

E

I

L

N
x
S

R
x
T

N

E

D

E

I

I

E

K

L

D

I

L

F

L

E

Q

T

T

A

A

D

Q

N

E

F

L

K

K

E

-

V

-

I

-

N

-

R

-

P

S

I

G

K

K

K

T

V

D

P

N

S

Q

L

L

R

T

D

G

I

K

T

F

I

A

A

A

N

N

I

L

F

F

E

E

N

P

S

S

T

T

R
|
R

T
|
T

K

R

L

F

S

S

F

F

E

E

L

V

A

A

E

E

K

K

R

K

L

L

S

G

A

M

D

N

V

V

V

L

N

N

F

L

A

D

A

G

S

T

S

S

S
x
T

S
|
S

V

V

S

Q

K
|
K

G

G

E

E

T

T

L

L

I

Y

D

D

T

T

V

I

N

R

N

T

I

L

L

E

S

S

M

I

K

G

V

V

D

D

M

V

V

C

V

V

M

I

R
|
R

H

H

P

S

Y

E

A

D

G

E

A

Y

G

Y

Q

E

F

E

L

L

S

V

K

S

H

Q

V

V

K

N

A

I

Q

P

I

I

V

L

N

N

A

A

G

G

D

D

G

G

A

C

H

G

E

Q

H
|
H

P

P

T

T

Q
|
Q

A

S

L

L

D

D

A

L

F

M

S

T

I

I

R

Y

Q

E

K

E

L

F

G

N

D

T

V

F

A

K

G

G

K

L

K

T

V

V

V

S

I

I

V

H

G

G

D

D

I

I

L

K

H

H

S

S

R

R

V

V

A

A

I

R

S

S

N

N

I

A

L

E

C

V

L

L

Q

T

R
|
R

L

L

G

G

A

A

E

R

V

V

M

L

V

F

C

S

G

G

P

P

T

S

T

E

L

W

I

Q

P

D

K

E

H

E

I

-

H

N

Q

T

L

F

G

G

V

T

K

Y

V

V

E

-

H

-

N

S

L

M

I

D

K

E

A

A

L

V

N

E

W

S

C

S

D

D

V

V

A

V

N

M

M

L

L
|
L

R

R

I

I

Q

Q

L

N

E

E

R

R

Q

H

D

-

I

-

K

Q

Y

S

F

A

P

V

S

S

L

Q

R

E

E

G

Y

Y

A

L

M

N

M

K

Y

Y

G

G

L

L

N

T

K

V

Q

E

I

R

L

A

D

E

N

R

L

M

D

K

K

R

E

H

I

A

I

I

V

I

M

M

H

H

P
|
P

G

A

P

P

I

V

N

N

R

R

G

G

V

V

E

E

I

I

T

D

S

D

D

S

V

L

A

V

D

E

S

S

K

E

Q

K

S

S

I

R

I

I

L

F

E

K

Q

Q

V

M

E

K

N

N

G
|
G

V

V

A

F

V

I

R

R

M

M

A

A

V

V

L

I

active site: R49 (= R63), T50 (= T64), K77 (= K91), R99 (= R113), H127 (= H141), Q130 (= Q144), L210 (= L227), P249 (= P268), G277 (= G296)
binding phosphate ion: S11 (≠ N20), T12 (≠ R21), T73 (≠ S87), S74 (= S88), K77 (= K91), R171 (= R185)

5g1nE Aspartate transcarbamoylase domain of human cad bound to pala (see paper)
36% identity, 95% coverage: 11:304/311 of query aligns to 6:300/307 of 5g1nE

query
sites
5g1nE

R

Q

H

H

L

I

L

L

G

S

I

V

K

Q

D

Q

I

F

N

T

R

K

N

D

D

Q

I

M

E

S

L

H

I

L

F

F

E

N

T

V

A

A

D

H

N

T

F

L

K

R

E

M

V

M

I

V

N

Q

R

K

P

E

I

-

K

R

K

S

V

L

P

D

S

I

L

L

R

K

D

G

I

K

T

V

I

M

A

A

N

S

I

M

F

F

F

Y

E

E

N

V

S
|
S

T
|
T

R
|
R

T
|
T

K

S

L

S

S

S

F

F

E

A

L

A

A

A

E

M

K

A

R

R

L

L

S

G

A

G

D

A

V

V

V

L

N

S

F

F

A

S

A

E

S

A

S

T

S

S

S
|
S

V

V

S

Q

K
|
K

G

G

E

E

T

S

L

L

I

A

D

D

T

S

V

V

N

Q

N

T

I

-

L

M

S

S

M

C

K

Y

V

A

D

D

M

V

V

V

M

L

R
|
R

H

H

P

P

Y

Q

A

P

G

G

A

A

G

V

Q

E

F

L

L

A

S

A

K

K

H

H

V

C

K

R

A

R

Q

P

I

V

V

I

N

N

A

A

G

G

D

D

G

G

A

V

H

G

E

E

H
|
H

P

P

T

T

Q
|
Q

A

A

L

L

D

D

A

I

F

F

S

T

I

I

R

R

Q

E

K

E

L

L

G

G

D

T

V

V

A

N

G

G

K

M

K

T

V

I

V

T

I

M

V

V

G

G

D

D

I

L

L

K

H

H

S

G

R
|
R

V

T

A

-

I

V

S

H

N

S

I

L

L

A

C

C

L

L

-

L

-

T

Q

Q

R

Y

L

R

G

V

A

S

E

L

V

R

M

Y

V

V

C

A

G

P

P

P

T

S

T

L

L

R

I

M

P

P

K

P

H

T

I

V

H

R

Q

A

L

F

-

V

-

A

-

S

-

R

G

G

V

T

K

K

V

Q

E

E

-

E

-

F

H

E

N

S

L

I

I

E

K

E

A

A

L

L

N

P

W

D

C

T

D

D

V

V

A

L

N

Y

M

M

L
x
T

R
|
R

I

I

Q
|
Q

L

K

E

E

R

R

Q

-

D

-

I

-

K

-

Y

-

F

F

P

G

S

S

L

T

R

Q

E

E

Y

Y

A

E

M

A

M

C

Y

F

G

G

-

Q

-

F

-

I

L

L

N

T

K

P

Q

H

I

I

L

M

D

T

N

R

L

A

D

K

K

K

E

K

I

M

I

V

V

V

M

M

H

H

P
|
P

G
x
M

P

P

I

-

N

-

R

R

G

V

V

N

E

E

I

I

T

S

S

V

D

E

V

V

A

D

D

S

S

D

K

P

Q

R

S

A

I

A

I

Y

L

F

E

R

Q

Q

V

A

E

E

N

N

G
|
G

V

M

A

Y

V

I

R

R

M

M

A

A

V

L

L

L

active site: R57 (= R63), T58 (= T64), K85 (= K91), R106 (= R113), H134 (= H141), Q137 (= Q144), T227 (≠ L227), P266 (= P268), G292 (= G296)
binding n-(phosphonacetyl)-l-aspartic acid: S55 (= S61), T56 (= T62), R57 (= R63), T58 (= T64), S82 (= S88), K85 (= K91), R106 (= R113), H134 (= H141), R167 (= R174), R228 (= R228), Q230 (= Q230), M267 (≠ G269)

P08955 Multifunctional protein CAD; Carbamoyl phosphate synthetase 2-aspartate transcarbamylase-dihydroorotase; EC 6.3.5.5; EC 3.5.1.2; EC 6.3.4.16; EC 2.1.3.2; EC 3.5.2.3 from Mesocricetus auratus (Golden hamster) (see paper)
36% identity, 95% coverage: 8:304/311 of query aligns to 1921:2218/2225 of P08955

query
sites
P08955

L

L

S

V

T

G

R

Q

H

H

L

I

L

L

G

S

I

V

K

K

D

Q

I

F

N

T

R

K

N

D

D

Q

I

M

E

S

L

H

I

L

F

F

E

N

T

V

A

A

D

H

N

T

F

L

K

R

E

M

V

M

I

V

N

Q

R

K

P

E

I

-

K

R

K

S

V

L

P

D

S

I

L

L

R

K

D

G

I

K

T

V

I

M

A

A

N

S

I

M

F

F

F

Y

E

E

N

V

S

S

T

T

R

R

T

T

K

S

L

S

S

S

F

F

E

A

L

A

A

A

E

M

K

A

R

R

L

L

S

G

A

G

D

A

V

V

V

L

N

S

F

F

A

S

A

E

S

A

S

T

S

S

V

V

S

Q

K

K

G

G

E

E

T

S

L

L

I

A

D

D

T

S

V

V

N

Q

N

T

I

-

L

M

S

S

M

C

K

Y

V

A

D

D

M

V

V

V

M

L

R

R

H

H

P

P

Y

Q

A

P

G

G

A

A

G

V

Q

E

F

L

L

A

S

A

K

K

H

H

V

C

K

R

A

R

Q

P

I

V

V

I

N

N

A

A

G

G

D

D

G

G

A

V

H

G

E

E

H

H

P

P

T

T

Q

Q

A

A

L

L

D

D

A

I

F

F

S

T

I

I

R

R

Q

E

K

E

L

L

G

G

D

T

V

V

A

N

G

G

K

M

K

T

V

I

V

T

I

M

V

V

G

G

D

D

I

L

L

K

H

H

S

G

R

R

V

T

A

-

I

V

S

H

N

S

I

L

L

A

C

C

L

L

-

L

-

T

Q

Q

R

Y

L

R

G

V

A

S

E

L

V

R

M

Y

V

V

C

A

G

P

P

P

T

S

T

L

L

R

I

M

P

P

K

P

H

S

I

V

H

W

Q

D

L

F

-

V

-

A

-

S

-

R

G

G

V

T

K

K

V

Q

E

E

-

E

-

F

H

E

N

S

L

I

I

E

K

E

A

A

L

L

N

P

W

D

C

T

D

D

V

V

A

L

N

Y

M

M

L

T

R

R

I

I

Q

Q

L

K

E

E

R

R

Q

-

D

-

I

-

K

-

Y

-

F

F

P

G

S

S

L

T

R

Q

E

E

Y

Y

A

E

M

A

M

C

Y

F

G

G

-

Q

-

F

-

I

L

L

N

T

K

P

Q

H

I

I

L

M

D

T

N

R

L

A

D

K

K

K

E

K

I

M

I

V

V

V

M

M

H

H

P

P

G

M

P

P

I

-

N

-

R

R

G

V

V

N

E

E

I

I

T

S

S

V

D

E

V

V

A

D

D

S

S

D

K

P

Q

R

S

A

I

A

I

Y

L

F

E

R

Q

Q

V

A

E

E

N

N

G

G

V

M

A

Y

V

I

R

R

M

M

A

A

V

L

L

L

Sites not aligning to the query:

1406 modified: Phosphoserine; by PKA; S→A: No effect on enzyme kinetics.; S→D: Increases CPSase activity and reduces sensitivity to feedback inhibition by UTP.

1ml4A The pala-liganded aspartate transcarbamoylase catalytic subunit from pyrococcus abyssi (see paper)
37% identity, 95% coverage: 11:307/311 of query aligns to 5:303/307 of 1ml4A

query
sites
1ml4A

R

R

H

D

L

V

L

I

G

S

I

I

K

R

D

D

I

F

N

S

R

K

N

E

D

D

I

I

E

E

L

T

I

V

F

L

E

A

T

T

A

A

D

-

N

-

F

-

K

-

E

E

V

R

I

L

N

E

R

R

P

E

I

L

K

K

K

E

V

K

P

G

S

Q

L

L

-

E

-

Y

-

A

R

K

D

G

I

K

T

I

I

L

A

A

N

T

I

L

F

F

E

E

N

P

S
|
S

T
|
T

R
|
R

T
|
T

K

R

L

L

S

S

F

F

E

E

L

S

A

A

E

M

K

H

R

R

L

L

S

G

A

G

D

A

V

V

V

I

N

G

F

F

A

A

-

E

A

A

S

S

S

T

S

S

V

V

S

K

K
|
K

G

G

E

E

T

S

L

L

I

R

D

D

T

T

V

I

N

K

N

T

I

V

L

-

S

E

M

Q

K

Y

V

C

D

D

M

V

V

I

V

V

M

I

R
|
R

H

H

P

P

Y

K

A

E

G

G

A

A

G

A

Q

R

F

L

L

A

S

A

K

E

H

V

V

A

K

E

A

V

Q

P

I

V

V

I

N

N

A

A

G

G

D

D

G

G

A

S

H

N

E

Q

H
|
H

P

P

T

T

Q
|
Q

A

T

L

L

D

D

A

L

F

Y

S

T

I

I

R

K

Q

K

K

E

L

F

G

G

D

R

V

I

A

D

G

G

K

L

K

K

V

I

V

G

I

L

V

L

G

G

D

D

I

L

L

K

H

Y

S

G

R
|
R

V
x
T

A

V

I

H

S

S

N

L

I

A

L

E

C

A

L

L

Q

T

R

F

L

Y

G

D

A

V

E

E

V

L

M

Y

V

L

C

I

G

S

P

P

T

E

T

L

L

L

-

R

I

M

P

P

K

R

H

H

I

I

-

V

-

E

-

L

H

R

Q

E

L

K

G

G

V

M

K

K

V

V

-

V

-

E

E

T

H

T

N

T

L

L

I

E

K

D

A

V

L

I

N

G

W

K

C

L

D

D

V

V

A

L

N

Y

M

V

L
x
T

R
|
R

I

I

Q

Q

L

K

E

E

R

R

Q

-

D

-

I

-

K

-

Y

-

F

F

P

P

S

D

L

E

R

Q

E

E

Y

Y

A

L

M

K

M

V

-

K

-

G

-

S

Y

Y

G

Q

L

V

N

N

K

L

Q

K

I

V

L

L

D

E

N

K

L

A

D

K

K

D

E

E

I

L

I

R

V

I

M

M

H

H

P
|
P

G
x
L

P

P

I

-

N

-

R

R

G

V

V

D

E

E

I

I

T

H

S

P

D

E

V

V

A

D

D

N

S

T

K

K

Q

H

S

A

I

I

I

Y

L

F

E

R

Q

Q

V

V

E

F

N

N

G
|
G

V

V

A

P

V

V

R

R

M

M

A

A

V

L

L

L

Y

A

L

L

L

V

active site: R56 (= R63), T57 (= T64), K85 (= K91), R106 (= R113), H134 (= H141), Q137 (= Q144), T227 (≠ L227), P266 (= P268), G292 (= G296)
binding n-(phosphonacetyl)-l-aspartic acid: S54 (= S61), T55 (= T62), R56 (= R63), T57 (= T64), R106 (= R113), H134 (= H141), R167 (= R174), T168 (≠ V175), R228 (= R228), L267 (≠ G269)

P27708 Multifunctional protein CAD; Carbamoyl phosphate synthetase 2-aspartate transcarbamylase-dihydroorotase; EC 6.3.5.5; EC 3.5.1.2; EC 6.3.4.16; EC 2.1.3.2; EC 3.5.2.3 from Homo sapiens (Human) (see 7 papers)
36% identity, 95% coverage: 8:304/311 of query aligns to 1921:2218/2225 of P27708

query
sites
P27708

L
|
L

S
x
V

T
x
G

R
x
Q

H
|
H

L
x
I

L
|
L

G
x
S

I
x
V

K
x
Q

D
x
Q

I
x
F

N
x
T

R
x
K

N
x
D

D
x
Q

I
x
M

E
x
S

L
x
H

I
x
L

F
|
F

E
x
N

T
x
V

A
|
A

D
x
H

N
x
T

F
x
L

K
x
R

E
x
M

V
x
M

I
x
V

N
x
Q

R
x
K

P
x
E

I

-

K
x
R

K
x
S

V
x
L

P
x
D

S
x
I

L
|
L

R
x
K

D
x
G

I
x
K

T
x
V

I
x
M

A
|
A

N
x
S

I
x
M

F
|
F

F
x
Y

E
|
E

N
x
V

S
|
S

T
|
T

R
|
R

T
|
T

K
x
S

L
x
S

S
|
S

F
|
F

E
x
A

L
x
A

A
|
A

E
x
M

K
x
A

R
|
R

L
|
L

S
x
G

A
x
G

D
x
A

V
|
V

V
x
L

N
x
S

F
|
F

A
x
S

A
x
E

S
x
A

S
x
T

S
|
S

V
|
V

S
x
Q

K
|
K

G
|
G

E
|
E

T
x
S

L
|
L

I
x
A

D
|
D

T
x
S

V
|
V

N
x
Q

N
x
T

I

-

L
x
M

S
|
S

M
x
C

K
x
Y

V
x
A

D
|
D

M
x
V

V
|
V

M
x
L

R
|
R

H
|
H

P
|
P

Y
x
Q

A
x
P

G
|
G

A
|
A

G
x
V

Q
x
E

F
x
L

L
x
A

S
x
A

K
|
K

H
|
H

V
x
C

K
x
R

A
x
R

Q
x
P

I
x
V

V
x
I

N
|
N

A
|
A

G
|
G

D
|
D

G
|
G

A
x
V

H
x
G

E
|
E

H
|
H

P
|
P

T
|
T

Q
|
Q

A
|
A

L
|
L

D
|
D

A
x
I

F
|
F

S
x
T

I
|
I

R
|
R

Q
x
E

K
x
E

L
|
L

G
|
G

D
x
T

V
|
V

A
x
N

G
|
G

K
x
M

K
x
T

V
x
I

V
x
T

I
x
M

V
|
V

G
|
G

D
|
D

I
x
L

L
x
K

H
|
H

S
x
G

R
|
R

V
x
T

A

-

I
x
V

S
x
H

N
x
S

I
x
L

L
x
A

C
|
C

L
|
L

-
x
L

-
x
T

Q
|
Q

R
x
Y

L
x
R

G
x
V

A
x
S

E
x
L

V
x
R

M
x
Y

V
|
V

C
x
A

G
x
P

P
|
P

T
x
S

T
x
L

L
x
R

I
x
M

P
|
P

K
x
P

H
x
T

I
x
V

H
x
R

Q
x
A

L
x
F

-
x
V

-
x
A

-
x
S

-
x
R

G
|
G

V
x
T

K
|
K

V
x
Q

E
|
E

-
x
E

-
x
F

H
x
E

N
x
S

L
x
I

I
x
E

K
x
E

A
|
A

L
|
L

N
x
P

W
x
D

C
x
T

D
|
D

V
|
V

A
x
L

N
x
Y

M
|
M

L
x
T

R
|
R

I
|
I

Q
|
Q

L
x
K

E
|
E

R
|
R

Q

-

D

-

I

-

K

-

Y

-

F
|
F

P
x
G

S
|
S

L
x
T

R
x
Q

E
|
E

Y
|
Y

A
x
E

M
x
A

M
x
C

Y
x
F

G
|
G

-
x
Q

-
x
F

-
x
I

L
|
L

N
x
T

K
x
P

Q
x
H

I
|
I

L
x
M

D
x
T

N
x
R

L
x
A

D
x
K

K
|
K

E
x
K

I
x
M

I
x
V

V
|
V

M
|
M

H
|
H

P
|
P

G
x
M

P
|
P

I

-

N

-

R
|
R

G
x
V

V
x
N

E
|
E

I
|
I

T
x
S

S
x
V

D
x
E

V
|
V

A
x
D

D
x
S

S
x
D

K
x
P

Q
x
R

S
x
A

I
x
A

I
x
Y

L
x
F

E
x
R

Q
|
Q

V
x
A

E
|
E

N
|
N

G
|
G

V
x
M

A
x
Y

V
x
I

R
|
R

M
|
M

A
|
A

V
x
L

L
|
L

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 modified: N-acetylalanine
33 M → R: in DEE50; uncertain significance; dbSNP:rs751610198
177 R → Q: in a colorectal cancer sample; somatic mutation; dbSNP:rs374122292
456 modified: Phosphothreonine; by MAPK1
735 Y → C: in a colorectal cancer sample; somatic mutation
1406 modified: Phosphoserine; by PKA
1471 binding Zn(2+); binding Zn(2+); H→A: No zinc-binding and no catalytic activity.; H→N: Abolishes dihydroorotase activity.
1473 binding Zn(2+); H→A: No zinc-binding and no catalytic activity.
1475 binding (S)-dihydroorotate
1505 binding (S)-dihydroorotate
1512 D→N: No change in catalytic activity.
1556 binding via carbamate group; binding via carbamate group; modified: N6-carboxylysine
1562 T→A: Abolishes dihydroorotase activity.
1563 F→A: Abolishes dihydroorotase activity.
1590 binding Zn(2+); H→A: Abolishes dihydroorotase activity.; H→N: No catalytic activity.
1613 binding Zn(2+); C→S: Reduces dihydroorotase activity.
1614 binding Zn(2+); H→A: Abolishes dihydroorotase activity.
1637 binding Zn(2+); E→T: Abolishes dihydroorotase activity.
1642 H→N: 11.5% of wild-type catalytic activity.
1661 binding (S)-dihydroorotate
1686 binding Zn(2+); D→N: Abolishes dihydroorotase activity.
1690 binding (S)-dihydroorotate; H→N: 3% of wild-type catalytic activity.
1702 binding (S)-dihydroorotate
1789:2225 natural variant: Missing (in DEE50; uncertain significance)
1859 modified: Phosphoserine; by RPS6KB1 and PKA
1873 modified: Phosphoserine; by PKC; in vitro; S→A: Abolishes PMA-induced Thr-456 phosphorylation.
1900 modified: Phosphoserine

5g1pA Aspartate transcarbamoylase domain of human cad bound to carbamoyl phosphate (see paper)
35% identity, 95% coverage: 11:304/311 of query aligns to 3:285/292 of 5g1pA

query
sites
5g1pA

R

Q

H

H

L

I

L

L

G

S

I

V

K

Q

D

Q

I

F

N

T

R

K

N

D

D

Q

I

M

E

S

L

H

I

L

F

F

E

N

T

V

A

A

D

H

N

T

F

L

K

R

E

M

V

M

I

V

N

Q

R

K

P

E

I

-

K

R

K

S

V

L

P

D

S

I

L

L

R

K

D

G

I

K

T

V

I

M

A

A

N

S

I

M

F

F

F

Y

E

E

N

V

S
|
S

T
|
T

R
|
R

T
|
T

K

S

L

S

S

S

F

F

E

A

L

A

A

A

E

M

K

A

R

R

L

L

S

G

A

G

D

A

V

V

V

L

N

S

F

F

A

S

A

E

S

A

S

T

S

S

V

V

S

Q

K
|
K

G

G

E

E

T

S

L

L

I

A

D

D

T

S

V

V

N

Q

N

T

I

-

L

M

S

S

M

C

K

Y

V

A

D

D

M

V

V

V

M

L

R
|
R

H

H

P

P

Y

Q

A

P

G

G

A

A

G

V

Q

E

F

L

L

A

S

A

K

K

H

H

V

C

K

R

A

R

Q

P

I

V

V

I

N

N

A

A

G

G

D

D

G

G

A

V

H

G

E

E

H
|
H

P

P

T

T

Q
|
Q

A

A

L

L

D

D

A

I

F

F

S

T

I

I

R

R

Q

E

K

E

L

L

G

G

D

T

V

V

A

N

G

G

K

M

K

T

V

I

V

T

I

M

V

V

G

G

D

D

I

L

L

K

H

H

S

G

R

R

V

T

A

-

I

V

S

H

N

S

I

L

L

A

C

C

L

L

-

L

-

T

Q

Q

R

Y

L

R

G

V

A

S

E

L

V

R

M

Y

V

V

C

A

G

P

P

P

T

S

T

L

L

R

I

M

P

P

K

P

H

T

I

V

H

R

Q

A

L

F

-

V

-

A

-

S

G

G

V

T

K

K

V

Q

E

E

-

E

-

F

H

E

N

S

L

I

I

E

K

E

A

A

L

L

N

P

W

D

C

T

D

D

V

V

A

L

N

Y

M

M

L
x
T

R

R

I

I

Q

Q

L

K

E

E

R

R

Q

-

D

-

I

-

K

-

Y

-

F

-

P

-

S

-

L

-

R

-

E

-

Y

-

A

-

M

F

M

Q

Y

F

G

I

L

L

N

T

K

P

Q

H

I

I

L

M

D

T

N

R

L

A

D

K

K

K

E

K

I

M

I

V

V

V

M

M

H

H

P
|
P

G
x
M

P

P

I

-

N

-

R

R

G

V

V

N

E

E

I

I

T

S

S

V

D

E

V

V

A

D

D

S

S

D

K

P

Q

R

S

A

I

A

I

Y

L

F

E

R

Q

Q

V

A

E

E

N

N

G
|
G

V

M

A

Y

V

I

R

R

M

M

A

A

V

L

L

L

active site: R54 (= R63), T55 (= T64), K82 (= K91), R103 (= R113), H131 (= H141), Q134 (= Q144), T223 (≠ L227), P251 (= P268), G277 (= G296)
binding phosphoric acid mono(formamide)ester: S52 (= S61), T53 (= T62), R54 (= R63), T55 (= T64), R103 (= R113), Q134 (= Q144), M252 (≠ G269)

4eknB Structure of the catalytic chain of methanococcus jannaschii aspartate transcarbamoylase in a hexagonal crystal form (see paper)
37% identity, 95% coverage: 11:304/311 of query aligns to 2:296/304 of 4eknB

query
sites
4eknB

R

K

H

H

L

L

L

I

G

S

I

M

K

K

D

D

I

I

N

G

R

K

N

E

D

E

I

I

E

L

L

E

I

I

F

L

E

D

T

E

A

A

D

R

N

K

F

M

K

E

E

E

V

L

I

L

N

N

-

T

-

K

R

R

P

P

I

L

K

K

K

-

V

-

P

-

S

L

L

L

R

E

D

G

I

K

T

I

I

L

A

A

N

T

I

V

F

F

F

Y

E

E

N

P

S

S

T

T

R

R

T

T

K

R

L

L

S

S

F

F

E

E

L

T

A

A

E

M

K

K

R

R

L

L

S

G

A

G

D

E

V

V

V

I

N

T

F

M

A

T

-

D

A

L

S

K

S

S

V

V

S

A

K

K

G

G

E

E

T

S

L

L

I

I

D

D

T

T

V

I

N

R

N

-

I

V

L

I

S

S

M

G

K

Y

V

A

D

D

M

I

V

I

V

V

M

L

R

R

H

H

P

P

Y

S

A

E

G

G

A

A

G

A

Q

R

F

L

L

A

S

S

K

E

H

Y

V

S

K

Q

A

V

Q

P

I

I

V

I

N

N

A

A

G

G

D

D

G

G

A

S

H

N

E

Q

H

H

P

P

T

T

Q

Q

A

T

L

L

D

D

A

L

F

Y

S

T

I

I

R

M

Q

R

K

E

L

I

G

G

D

R

V

I

A

D

G

G

K

I

K

K

V

I

V

A

I

F

V

V

G

G

D

D

I

L

L

K

H

Y

S
x
G

R
|
R

V
x
T

A

V

I

H

S

S

N

L

I

V

L

Y

C

A

L

L

Q

S

R

L

L

F

-

E

G

N

A

V

E

E

V

M

M

Y

V

F

C

V

G

S

P

P

T

K

T

E

L

L

-

R

I

L

P

P

K

K

H

D

I

I

-

I

-

E

-

D

-

L

-

K

-

A

H

K

Q

N

L

I

G

K

V

F

K

Y

V

E

E

K

H

E

N

S

L

L

I

D

K

D

A

L

L

D

N

D

W

D

C

I

D

D

V

V

A

L

N

Y

M

V

L
x
T

R
|
R

I

I

Q

Q

L

K

E

E

R

R

Q

-

D

-

I

-

K

-

Y

-

F

F

P

P

S

D

L

P

R

N

E

E

Y

Y

A

E

M

K

M

V

Y

K

G

G

L

S

N

Y

K

K

Q

I

I

K

L

R

D

E

N

Y

L

V

D

E

-

G

K

K

E

K

I

F

I

I

V

I

M

M

H

H

P
|
P

G

L

P

P

I

-

N

-

R

R

G

V

V

D

E

E

I

I

T

D

S

Y

D

D

V

V

A

D

D

D

S

L

K

P

Q

Q

S

A

I

K

I

Y

L

F

E

K

Q

Q

V

S

E

F

N

Y

G
|
G

V

I

A

P

V

V

R

R

M

M

A

A

V

I

L

L

active site: T225 (≠ L227), P262 (= P268), G288 (= G296)
binding sulfate ion: G163 (≠ S173), R164 (= R174), T165 (≠ V175), R226 (= R228)

P20054 Multifunctional protein pyr1-3; EC 6.3.5.5; EC 3.5.1.2; EC 6.3.4.16; EC 2.1.3.2; EC 3.5.2.3 from Dictyostelium discoideum (Social amoeba)
34% identity, 98% coverage: 8:311/311 of query aligns to 1920:2224/2225 of P20054

query
sites
P20054

L

L

S

A

T

G

R

K

H

H

L

I

L

F

G

S

I

V

K

K

D

Q

I

F

N

N

R

R

N

K

D

Q

I

L

E

H

L

A

I

L

F

F

E

G

T

I

A

A

D

H

N

E

F

M

K

R

E

I

V

L

I

V

N

K

R

R

P

S

I

-

K

G

V

S

P

D

S

L

L

L

R

K

D

G

I

K

T

V

I

L

A

A

N

T

I

L

F

F

F

Y

E

E

N

P

S

S

T

T

R

R

T

T

K

Q

L

C

S

S

F

F

E

T

L

A

A

A

E

M

K

Q

R

R

L

L

S

G

A

G

D

S

V

V

N

T

F

V

A

D

A

N

S

V

S

S

V

V

S

A

K

K

G

G

E

E

T

S

L

I

I

A

D

D

T

T

V

I

N

Q

N

T

I

L

L

E

S

S

M

Y

K

-

V

C

D

D

M

A

V

V

V

C

M

M

R

R

H

H

P

P

Y

A

A

V

G

G

A

S

G

V

Q

E

F

S

L

A

S

I

K

Q

H

V

V

A

K

K

A

K

Q

P