SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing CA265_RS15205 FitnessBrowser__Pedo557:CA265_RS15205 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P42588 Putrescine aminotransferase; PAT; PATase; Cadaverine transaminase; Diamine transaminase; Putrescine transaminase; Putrescine--2-oxoglutaric acid transaminase; Putrescine:2-OG aminotransferase; EC 2.6.1.82; EC 2.6.1.29 from Escherichia coli (strain K12) (see paper)
41% identity, 95% coverage: 12:369/378 of query aligns to 76:443/459 of P42588

query
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P42588

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GT 150:151 (= GT 88:89) binding in other chain
Q274 (= Q202) binding in other chain
K300 (= K228) modified: N6-(pyridoxal phosphate)lysine
T332 (= T260) binding pyridoxal 5'-phosphate

4uoyA Crystal structure of ygjg in complex with pyridoxal-5'-phosphate (see paper)
41% identity, 97% coverage: 12:377/378 of query aligns to 70:445/454 of 4uoyA

query
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active site: F174 (≠ Y115), E232 (= E166), D265 (= D199), Q268 (= Q202), K294 (= K228), T326 (= T260), R420 (= R348)
binding pyridoxal-5'-phosphate: G144 (= G88), T145 (= T89), F174 (≠ Y115), H175 (= H116), G176 (= G117), E232 (= E166), D265 (= D199), V267 (≠ I201), Q268 (= Q202), K294 (= K228)

Sites not aligning to the query:

active site: 44

8cplC Yzw2 a scaffold for cryo-em of small proteins of interest
41% identity, 95% coverage: 12:369/378 of query aligns to 68:435/499 of 8cplC

query
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binding pyridoxal-5'-phosphate: S141 (= S87), G142 (= G88), T143 (= T89), F172 (≠ Y115), H173 (= H116), E230 (= E166), D263 (= D199), V265 (≠ I201), Q266 (= Q202), K292 (= K228)

4uoxA Crystal structure of ygjg in complex with pyridoxal-5'-phosphate and putrescine (see paper)
41% identity, 95% coverage: 12:369/378 of query aligns to 70:437/453 of 4uoxA

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S

L

A

M

T

E

A

S

K

D

S

F

T

Y

F

S

R

S

K

G

P

Y

F

G

M

P

P

F

L

L

L

P

P

-

G

-

F

-

R

H

H

V

V

S

P

F

F

-

G

-

N

I

I

E

E

-

A

-

M

H

R

N

T

N

A

L

L

A

N

D

E

L

C

E

K

K

K

I

T

T

G

N

D

E

D

I

V

A

A

V

V

F

I

I

L

E
|
E

P

P

I

I

Q

Q

G

G

E
|
E

A

G

G

G

I

V

R

I

V

L

S

P

D

P

L

P

S

G

Y

Y

M

L

Q

T

A

A

L

V

R

R

T

K

K

L

C

C

T

D

E

E

T

F

G

G

T

A

L

L

L

M

I

I

F

L

D
|
D

E

E

I
x
V

Q
|
Q

S

T

G

G

F

M

G

G

R

R

S

T

G

G

K

K

M

M

F

F

A

A

F

C

E

E

H

H

Y

E

N

N

V

V

V

Q

P

P

D

D

V

I

L

L

L

C

L

L

A

A

K
|
K

G

A

I

L

G

G

-

V

M

M

P

P

I

I

G

G

A

A

F

T

I

I

S

A

S

T

L

E

E

E

I

V

M

F

S

S

V

V

L

L

S

F

H

D

T

N

P

P

I

F

L

L

G

-

H

H

M

T

T
|
T

F

F

G

G

H

N

P

P

V

L

C

A

C

C

A

A

G

A

L

L

A

A

T

T

L

I

R

N

T

V

L

L

V

L

D

E

D

Q

H

N

I

L

V

P

D

A

E

Q

V

A

E

E

E

Q

K

K

G

G

Q

D

L

M

-

L

-

D

-

G

F

F

K

R

Q

Q

L

L

L

A

-

R

Q

E

H

Y

P

P

-

D

A

L

I

V

K

Q

E

E

I

A

R

R

G

G

K

K

G

G

L

M

M

L

L

M

A

A

V

I

E

E

F

F

E

V

N

D

F

N

E

E

I

I

N

G

K

Y

K

N

I

F

I

A

D

S

A

E

C

M

I

F

L

R

D

Q

G

R

V

V

L

L

S

V

D

A

W

G

F

T

L

L

H

N

C

N

S

A

N

K

S

T

M

I

R
|
R

I

I

A

E

P

P

L

L

I

T

I

L

T

T

K

I

E

E

E

Q

I

-

A

-

E

-

A

-

C

C

T

E

I

L

I

V

L

I

K

K

active site: F174 (≠ Y115), E232 (= E166), D265 (= D199), Q268 (= Q202), K294 (= K228), T326 (= T260), R420 (= R348)
binding pyridoxal-5'-phosphate: S143 (= S87), G144 (= G88), T145 (= T89), F174 (≠ Y115), H175 (= H116), G176 (= G117), D265 (= D199), V267 (≠ I201), Q268 (= Q202), T325 (= T259), T326 (= T260)
binding 1,4-diaminobutane: E237 (= E171), K294 (= K228)

Sites not aligning to the query:

active site: 44

Q5SHH5 [LysW]-aminoadipate semialdehyde transaminase; EC 2.6.1.118 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)
39% identity, 97% coverage: 2:368/378 of query aligns to 29:394/395 of Q5SHH5

query
sites
Q5SHH5

L

L

E

L

F

I

V

V

R

R

A

G

K

Q

G

G

I

A

Y

R

I

V

Y

W

D

D

A

A

Q

E

N

G

K

N

K

E

H

Y

I

I

D

D

L

C

I

V

A

G

G

G

I

Y

G

G

V

V

S

A

N

N

V

L

G

G

H

H

C

G

H

N

P

P

A

E

V

V

K

E

A

A

I

V

Q

K

E

R

Q

Q

A

A

E

E

T

T

Y

-

M

-

H

-

L

L

M

M

V

A

Y

M

G

P

E

Q

Y

T

V

L

Q

P

T

T

P

P

-

M

Q

R

V

G

N

E

F

F

A

Y

K

R

A

T

L

L

A

T

D

A

I

I

L

L

P

P

E

P

S

E

L

L

S

N

C

R

T

V

Y

F

F

P

L

V

N

N

S

S

G
|
G

T
|
T

E

E

A

A

V

N

E

E

G

A

A

A

M

L

K

K

L

F

A

A

K

R

R

A

Y

H

T

T

G

G

R

R

K

K

G

K

F

F

I

V

A

A

C

A

K

M

N

R

A

G

Y

F

H

S

G

G

S

R

T

T

Q

M

G

G

A

S

E

L

S

S

L

V

M

T

E

W

S

E

D

P

F

K

Y

Y

S

R

S

E

G

P

Y

F

G

L

P

P

F

L

L

V

P

E

H

P

V

V

S

E

F

F

I

I

E

P

H

Y

N

N

N

D

L

V

A

E

D

A

L

L

E

K

K

R

-

A

I

V

T

D

N

E

E

E

I

T

A

A

V

V

F

I

I

L

E

E

P

P

I

V

Q

Q

G

G

E

E

A

G

G

G

I

V

R

R

V

P

S

A

D

T

L

P

S

E

Y

F

M

L

Q

R

A

A

L

A

R

R

T

E

K

I

C

T

T

Q

E

E

T

K

G

G

T

A

L

L

I

I

F

L

D

D

E

E

I

I

Q

Q

S

T

G

G

F

M

G

G

R

R

S

T

G

G

K

K

M

R

F

F

A

A

F

F

E

E

H

H

Y

F

N

G

V

I

V

V

P

P

D

D

V

I

L

L

L

T

L

L

A

A

K
|
K

G

A

I

L

G

G

M

V

P

P

I

L

G

G

A

A

F

A

I

V

S

M

S

R

L

E

E

E

I

V

M

A

S

R

V

S

L

M

S

-

H

-

T

-

P

P

I

K

L

G

G

G

H

H

M

G

T

T

T
|
T

F

F

G

G

H

N

P

P

V

L

C

A

C

M

A

A

G

G

L

V

A

A

T

A

L

I

R

R

T

Y

L

L

V

E

D

R

D

T

H

R

I

L

V

W

D

E

E

R

V

A

E

A

E

E

K

L

G

G

Q

P

L

W

F

F

K

M

Q

E

L

K

L

L

Q

R

-

A

-

I

-

P

H

S

P

P

A

K

I

I

K

R

E

E

I

V

R

R

G

G

K

M

G

G

L

L

M

M

L

V

A

G

V

L

E

E

F

L

E

K

N

-

F

-

E

-

I

-

N

E

K

K

K

A

I

A

I

P

D

Y

A

I

C

A

I

R

L

L

D

E

G

K

V

E

L

H

S

R

D

V

W

L

F

A

L

L

H

Q

C

A

S

G

N

P

S

T

-

V

M

I

R

R

I

F

A

L

P

P

L

L

I

V

I

I

T

E

K

K

E

E

E

D

-

L

-

E

-

R

I

V

A

V

E

E

A

A

C

V

T

R

I

A

I

V

L

L

GT 113:114 (= GT 88:89) binding pyridoxal 5'-phosphate
K254 (= K228) modified: N6-(pyridoxal phosphate)lysine
T283 (= T260) binding pyridoxal 5'-phosphate

1wkhA Acetylornithine aminotransferase from thermus thermophilus hb8
39% identity, 97% coverage: 2:368/378 of query aligns to 21:386/387 of 1wkhA

query
sites
1wkhA

L

L

E

L

F

I

V

V

R

R

A

G

K

Q

G

G

I

A

Y

R

I

V

Y

W

D

D

A

A

Q

E

N

G

K

N

K

E

H

Y

I

I

D

D

L

C

I

V

A

G

G

G

I
x
Y

G

G

V

V

S

A

N

N

V

L

G

G

H

H

C

G

H

N

P

P

A

E

V

V

K

E

A

A

I

V

Q

K

E

R

Q

Q

A

A

E

E

T

T

Y

-

M

-

H

-

L

L

M

M

V

A

Y

M

G

P

E

Q

Y

T

V

L

Q

P

T

T

P

P

-

M

Q

R

V

G

N

E

F

F

A

Y

K

R

A

T

L

L

A

T

D

A

I

I

L

L

P

P

E

P

S

E

L

L

S

N

C

R

T

V

Y

F

F

P

L

V

N

N

S
|
S

G
|
G

T
|
T

E

E

A

A

V

N

E

E

G

A

A

A

M

L

K

K

L

F

A

A

K

R

R

A

Y

H

T

T

G

G

R

R

K

K

G

K

F

F

I

V

A

A

C

A

K

M

N

R

A

G

Y
x
F

H
x
S

G

G

S

R

T

T

Q

M

G

G

A

S

E

L

S

S

L

V

M

T

E

W

S

E

D

P

F

K

Y

Y

S

R

S

E

G

P

Y

F

G

L

P

P

F

L

L

V

P

E

H

P

V

V

S

E

F

F

I

I

E

P

H

Y

N

N

N

D

L

V

A

E

D

A

L

L

E

K

K

R

-

A

I

V

T

D

N

E

E

E

I

T

A

A

V

V

F

I

I

L

E
|
E

P

P

I

V

Q

Q

G

G

E
|
E

A

G

G

G

I

V

R

R

V

P

S

A

D

T

L

P

S

E

Y

F

M

L

Q

R

A

A

L

A

R

R

T

E

K

I

C

T

T

Q

E

E

T

K

G

G

T

A

L

L

I

I

F

L

D
|
D

E

E

I
|
I

Q
|
Q

S

T

G

G

F

M

G

G

R

R

S

T

G

G

K

K

M

R

F

F

A

A

F

F

E

E

H

H

Y

F

N

G

V

I

V

V

P

P

D

D

V

I

L

L

L

T

L

L

A

A

K
|
K

G

A

I

L

G

G

M

V

P

P

I

L

G

G

A

V

F

A

I

V

S

M

S

R

L

E

E

E

I

V

M

A

S

R

V

S

L

M

S

-

H

-

T

-

P

P

I

K

L

G

G

G

H

H

M

G

T

T

T
|
T

F

F

G

G

H

N

P

P

V

L

C

A

C

M

A

A

G

G

L

V

A

A

T

A

L

I

R

R

T

Y

L

L

V

E

D

R

D

T

H

R

I

L

V

W

D

E

E

R

V

A

E

A

E

E

K

L

G

G

Q

P

L

W

F

F

K

M

Q

E

L

K

L

L

Q

R

-

A

-

I

-

P

H

S

P

P

A

K

I

I

K

R

E

E

I

V

R

R

G

G

K

M

G

G

L

L

M

M

L

V

A

G

V

L

E

E

F

L

E

K

N

-

F

-

E

-

I

-

N

E

K

K

K

A

I

A

I

P

D

Y

A

I

C

A

I

R

L

L

D

E

G

K

V

E

L

H

S

R

D

V

W

L

F

A

L

L

H

Q

C

A

S

G

N

P

S

T

-

V

M

I

R
|
R

I

F

A

L

P

P

L

L

I

V

I

I

T

E

K

K

E

E

E

D

-

L

-

E

-

R

I

V

A

V

E

E

A

A

C

V

T

R

I

A

I

V

L

L

active site: F132 (≠ Y115), E184 (= E166), D217 (= D199), Q220 (= Q202), K246 (= K228), T275 (= T260), R363 (= R348)
binding 4-[(1,3-dicarboxy-propylamino)-methyl]-3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridinium: Y46 (≠ I27), S104 (= S87), G105 (= G88), T106 (= T89), F132 (≠ Y115), S133 (≠ H116), E184 (= E166), E189 (= E171), D217 (= D199), I219 (= I201), K246 (= K228), R363 (= R348)

Sites not aligning to the query:

active site: 13

1wkgA Acetylornithine aminotransferase from thermus thermophilus hb8
39% identity, 97% coverage: 2:368/378 of query aligns to 21:386/387 of 1wkgA

query
sites
1wkgA

L

L

E

L

F

I

V

V

R

R

A

G

K

Q

G

G

I

A

Y

R

I

V

Y

W

D

D

A

A

Q

E

N

G

K

N

K

E

H

Y

I

I

D

D

L

C

I

V

A

G

G

G

I
x
Y

G

G

V

V

S

A

N

N

V

L

G

G

H

H

C

G

H

N

P

P

A

E

V

V

K

E

A

A

I

V

Q

K

E

R

Q

Q

A

A

E

E

T

T

Y

-

M

-

H

-

L

L

M

M

V

A

Y

M

G

P

E

Q

Y

T

V

L

Q

P

T

T

P

P

-

M

Q

R

V

G

N

E

F

F

A

Y

K

R

A

T

L

L

A

T

D

A

I

I

L

L

P

P

E

P

S

E

L

L

S

N

C

R

T

V

Y

F

F

P

L

V

N

N

S

S

G
|
G

T
|
T

E

E

A

A

V

N

E

E

G

A

A

A

M

L

K

K

L

F

A

A

K

R

R

A

Y

H

T

T

G

G

R

R

K

K

G

K

F

F

I

V

A

A

C

A

K

M

N

R

A

G

Y
x
F

H
x
S

G

G

S
x
R

T

T

Q

M

G

G

A

S

E

L

S

S

L

V

M

T

E

W

S

E

D

P

F

K

Y

Y

S

R

S

E

G

P

Y

F

G

L

P

P

F

L

L

V

P

E

H

P

V

V

S

E

F

F

I

I

E

P

H

Y

N

N

N

D

L

V

A

E

D

A

L

L

E

K

K

R

-

A

I

V

T

D

N

E

E

E

I

T

A

A

V

V

F

I

I

L

E
|
E

P

P

I

V

Q

Q

G

G

E

E

A

G

G

G

I

V

R

R

V

P

S

A

D

T

L

P

S

E

Y

F

M

L

Q

R

A

A

L

A

R

R

T

E

K

I

C

T

T

Q

E

E

T

K

G

G

T

A

L

L

I

I

F

L

D
|
D

E

E

I
|
I

Q
|
Q

S

T

G

G

F

M

G

G

R

R

S

T

G

G

K

K

M

R

F

F

A

A

F

F

E

E

H

H

Y

F

N

G

V

I

V

V

P

P

D

D

V

I

L

L

L

T

L

L

A

A

K
|
K

G

A

I

L

G

G

M

V

P

P

I

L

G

G

A

V

F

A

I

V

S

M

S

R

L

E

E

E

I

V

M

A

S

R

V

S

L

M

S

-

H

-

T

-

P

P

I

K

L

G

G

G

H

H

M
x
G

T
|
T

F

F

G

G

H

N

P

P

V

L

C

A

C

M

A

A

G

G

L

V

A

A

T

A

L

I

R

R

T

Y

L

L

V

E

D

R

D

T

H

R

I

L

V

W

D

E

E

R

V

A

E

A

E

E

K

L

G

G

Q

P

L

W

F

F

K

M

Q

E

L

K

L

L

Q

R

-

A

-

I

-

P

H

S

P

P

A

K

I

I

K

R

E

E

I

V

R

R

G

G

K

M

G

G

L

L

M

M

L

V

A

G

V

L

E

E

F

L

E

K

N

-

F

-

E

-

I

-

N

E

K

K

K

A

I

A

I

P

D

Y

A

I

C

A

I

R

L

L

D

E

G

K

V

E

L

H

S

R

D

V

W

L

F

A

L

L

H

Q

C

A

S

G

N

P

S

T

-

V

M

I

R
|
R

I

F

A

L

P

P

L

L

I

V

I

I

T

E

K

K

E

E

E

D

-

L

-

E

-

R

I

V

A

V

E

E

A

A

C

V

T

R

I

A

I

V

L

L

active site: F132 (≠ Y115), E184 (= E166), D217 (= D199), Q220 (= Q202), K246 (= K228), T275 (= T260), R363 (= R348)
binding n~2~-acetyl-n~5~-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-l-ornithine: Y46 (≠ I27), G105 (= G88), T106 (= T89), F132 (≠ Y115), S133 (≠ H116), R135 (≠ S118), E184 (= E166), D217 (= D199), I219 (= I201), Q220 (= Q202), K246 (= K228), G273 (≠ M258), T274 (= T259), T275 (= T260)

Sites not aligning to the query:

1vefA Acetylornithine aminotransferase from thermus thermophilus hb8
39% identity, 97% coverage: 2:368/378 of query aligns to 21:386/387 of 1vefA

query
sites
1vefA

L

L

E

L

F

I

V

V

R

R

A

G

K

Q

G

G

I

A

Y

R

I

V

Y

W

D

D

A

A

Q

E

N

G

K

N

K

E

H

Y

I

I

D

D

L

C

I

V

A

G

G

G

I

Y

G

G

V

V

S

A

N

N

V

L

G

G

H

H

C

G

H

N

P

P

A

E

V

V

K

E

A

A

I

V

Q

K

E

R

Q

Q

A

A

E

E

T

T

Y

-

M

-

H

-

L

L

M

M

V

A

Y

M

G

P

E

Q

Y

T

V

L

Q

P

T

T

P

P

-

M

Q

R

V

G

N

E

F

F

A

Y

K

R

A

T

L

L

A

T

D

A

I

I

L

L

P

P

E

P

S

E

L

L

S

N

C

R

T

V

Y

F

F

P

L

V

N

N

S

S

G
|
G

T
|
T

E

E

A

A

V

N

E

E

G

A

A

A

M

L

K

K

L

F

A

A

K

R

R

A

Y

H

T

T

G

G

R

R

K

K

G

K

F

F

I

V

A

A

C

A

K

M

N

R

A

G

Y
x
F

H
x
S

G

G

S

R

T

T

Q

M

G

G

A

S

E

L

S

S

L

V

M

T

E

W

S

E

D

P

F

K

Y

Y

S

R

S

E

G

P

Y

F

G

L

P

P

F

L

L

V

P

E

H

P

V

V

S

E

F

F

I

I

E

P

H

Y

N

N

N

D

L

V

A

E

D

A

L

L

E

K

K

R

-

A

I

V

T

D

N

E

E

E

I

T

A

A

V

V

F

I

I

L

E
|
E

P

P

I

V

Q

Q

G

G

E

E

A

G

G

G

I

V

R

R

V

P

S

A

D

T

L

P

S

E

Y

F

M

L

Q

R

A

A

L

A

R

R

T

E

K

I

C

T

T

Q

E

E

T

K

G

G

T

A

L

L

I

I

F

L

D
|
D

E

E

I
|
I

Q

Q

S

T

G

G

F

M

G

G

R

R

S

T

G

G

K

K

M

R

F

F

A

A

F

F

E

E

H

H

Y

F

N

G

V

I

V

V

P

P

D

D

V

I

L

L

L

T

L

L

A

A

K
|
K

G

A

I

L

G

G

M

V

P

P

I

L

G

G

A

V

F

A

I

V

S

M

S

R

L

E

E

E

I

V

M

A

S

R

V

S

L

M

S

-

H

-

T

-

P

P

I

K

L

G

G

G

H

H

M

G

T

T

T
|
T

F

F

G

G

H

N

P

P

V

L

C

A

C

M

A

A

G

G

L

V

A

A

T

A

L

I

R

R

T

Y

L

L

V

E

D

R

D

T

H

R

I

L

V

W

D

E

E

R

V

A

E

A

E

E

K

L

G

G

Q

P

L

W

F

F

K

M

Q

E

L

K

L

L

Q

R

-

A

-

I

-

P

H

S

P

P

A

K

I

I

K

R

E

E

I

V

R

R

G

G

K

M

G

G

L

L

M

M

L

V

A

G

V

L

E

E

F

L

E

K

N

-

F

-

E

-

I

-

N

E

K

K

K

A

I

A

I

P

D

Y

A

I

C

A

I

R

L

L

D

E

G

K

V

E

L

H

S

R

D

V

W

L

F

A

L

L

H

Q

C

A

S

G

N

P

S

T

-

V

M

I

R
|
R

I

F

A

L

P

P

L

L

I

V

I

I

T

E

K

K

E

E

E

D

-

L

-

E

-

R

I

V

A

V

E

E

A

A

C

V

T

R

I

A

I

V

L

L

active site: F132 (≠ Y115), D217 (= D199), K246 (= K228), T275 (= T260), R363 (= R348)
binding pyridoxal-5'-phosphate: G105 (= G88), T106 (= T89), F132 (≠ Y115), S133 (≠ H116), E184 (= E166), D217 (= D199), I219 (= I201), K246 (= K228)

Sites not aligning to the query:

active site: 13

Q9M8M7 Acetylornithine aminotransferase, chloroplastic/mitochondrial; ACOAT; Acetylornithine transaminase; AOTA; Protein HOPW1-1-INTERACTING 1; EC 2.6.1.11 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
35% identity, 97% coverage: 8:374/378 of query aligns to 84:456/457 of Q9M8M7

query
sites
Q9M8M7

K

K

G

G

I

C

Y

K

I

L

Y

F

D

D

A

P

Q

E

N

G

K

K

K

E

H

Y

I

L

D

D

L

C

I

A

A

S

G

G

I

I

G

A

V

V

S

N

N

A

V

L

G

G

H

H

C

G

H

D

P

P

A

D

V

W

V

L

K

R

A

A

I

V

Q

T

E

E

Q

Q

A

A

E

G

T

V

Y

L

M

A

H

H

L

-

M

-

V

V

Y

S

G

N

E

V

Y

Y

V

Y

Q

T

T

I

P

P

Q

Q

V

I

N

E

F

L

A

A

K

K

A

R

L

L

A

-

D

-

I

V

L

A

P

S

E

S

S

F

L

A

S

D

C

R

T

V

Y

F

F

F

L

C

N

N

S

S

G

G

T

T

E

E

A

A

V

N

E

E

G

A

A

A

M

I

K

K

L

F

A

S

K

R

R

K

Y

F

T

Q

G

R

-

F

-

T

-

H

-

P

-

E

-

D

-

K

-

E

-

V

R

A

K

T

G

G

F

F

I

I

A

A

C

F

K

T

N

N

A

S

Y

F

H

H

G

G

S

R

T

T

Q

L

G

G

A

A

E

L

S

A

L

L

M

T

E

S

S

K

D

E

F

Q

Y

Y

S

R

S

T

G

P

Y

F

G

E

P

P

F

I

L

M

P

P

H

G

V

V

S

T

F

F

I

L

E

E

H

Y

N

G

N

N

L

I

-

Q

-

A

A

T

D

D

L

L

E

I

K

R

I

-

T

S

N

G

E

K

I

I

A

A

V

V

F

F

I

V

E

E

P

P

I

I

Q

Q

G

G

E

E

A

G

G

G

I

I

R

Y

V

S

S

A

D

T

L

K

S

E

Y

F

M

L

Q

Q

A

S

L

L

R

R

T

S

K

A

C

C

T

D

E

A

T

A

G

G

T

S

L

L

I

V

F

F

D

D

E

E

I

V

Q

Q

S

C

G

G

F

L

G

G

R

R

S

T

G

G

K

L

M

M

F

W

A

A

F

Y

E

E

H

A

Y

F

N

G

V

V

V

T

P

P

D

D

V

I

L

M

L

T

L

V

A

A

K

K

G

P

I

L

G

A

G

G

M

L

P

P

I

I

G

G

A

A

F

V

I

L

S

V

S

T

L

E

E

K

I

V

M

A

S

E

V

T

L

I

S

N

H

Y

T

G

P

-

I

-

L

-

G

D

H

H

M

G

T

S

T

T

F

F

G

A

G

G

H

S

P

P

V

L

C

V

C

C

A

S

A

A

G

A

L

I

A

A

T

V

L

M

R

D

T

K

L

V

V

S

D

K

D

P

H

S

I

F

V

L

D

S

E

S

V

V

E

S

E

N

K

K

G

G

Q

R

L

Y

F

F

K

R

Q

D

L

L

-

V

-

K

-

L

-

G

Q

G

H

N

P

S

A

H

I

V

K

K

E

E

I

V

R

R

G

G

K

E

G

G

L

L

M

I

L

I

A

G

V

V

E

E

F

L

E

D

N

-

F

-

E

V

I

P

N

A

K

S

I

L

I

V

D

D

A

A

C

C

I

R

L

D

D

S

G

G

V

L

L

L

S

I

D

-

W

L

F

T

L

A

H

G

C

K

S

G

N

N

S

V

M

V

R

R

I

I

A

V

P

P

L

L

I

V

I

I

T

S

K

E

E

E

I

I

A

E

E

R

A

A

C

V

T

E

I

I

I

M

L

S

K

Q

N

N

V

L

N

T

S

A

V

L

Sites not aligning to the query:

1:41 modified: transit peptide, Chloroplast and mitochondrion
42 modified: N-acetylvaline; in Acetylornithine aminotransferase, chloroplastic

8ht4B Crystal structure of acetylornithine aminotransferase complex with plp from corynebacterium glutamicum (see paper)
38% identity, 98% coverage: 2:371/378 of query aligns to 19:389/390 of 8ht4B

query
sites
8ht4B

L

V

E

E

F

L

V

V

R

S

A

G

K

K

G

G

I

A

Y

T

I

V

Y

T

D

D

A

D

Q

Q

N

G

K

N

K

V

H

Y

I

I

D

D

L

L

I

L

A

A

G

G

I

I

G

A

V

V

S

N

N

A

V

L

G

G

H

H

C

A

H

H

P

P

A

A

V

I

K

E

A

A

I

V

Q

T

E

N

Q

Q

A

I

E

G

T

Q

Y

L

M

G

H

H

L

-

M

-

V

V

Y

S

G

N

E

L

Y

F

V

A

Q

S

T

R

P

P

Q

V

V

V

N

E

F

V

A

A

K

E

-

E

-

L

-

I

-

K

-

R

-

F

A

S

L

L

A

D

D

D

I

A

L

T

P

L

E

A

S

A

L

Q

S

T

C

R

T

V

Y

F

F

F

L

C

N

N

S
|
S

G
|
G

T
x
A

E

E

A

A

V

N

E

E

G

A

A

A

M

F

K

K

L

I

A

A

K

-

R

R

Y

L

T

T

G

G

R

R

K

S

G

R

F

I

I

L

A

A

C

A

K

V

N

H

A

G

Y
x
F

H
|
H

G

G

S

R

T

T

Q

M

G

G

A

S

E

L

S

A

L

L

M

T

E

G

S

Q

D

P

F

D

Y

K

S

R

S

E

G

A

Y

F

G

L

P

P

F

M

L

P

P

S

H

G

V

V

S

E

F

F

I

Y

E

P

H

Y

N

G

N

D

L

T

A

D

D

Y

L

L

E

R

K

K

I

M

-

V

-

E

T

T

N

N

-

P

-

T

E

D

I

V

A

A

V

I

F

F

I

L

E
|
E

P

P

I

I

Q

Q

G

G

E

E

A

T

G

G

I

V

R

V

V

P

S

A

D

P

L

E

S

G

Y

F

M

L

Q

K

A

A

L

V

R

R

T

E

K

L

C

C

T

D

E

E

T

Y

G

G

T

I

L

L

L

M

I

I

F

T

D
|
D

E

E

I
x
V

Q
|
Q

S

T

G

G

F

V

G

G

R

R

S

T

G

G

K

D

M

F

F

F

A

A

F

H

E

Q

H

H

Y

D

N

G

V

V

P

P

D

D

V

V

L

V

L

T

L

M

A

A

K
|
K

G

G

I

L

G

G

M

L

P

P

I

I

G

G

A

A

F

C

I

L

S

A

S

T

L

G

E

R

I

A

M

A

S

E

V

L

L

M

S

T

H

P

T

G

P

K

I

-

L

-

G

-

H

H

M

G

T

T

F

F

G

G

H

N

P

P

V

V

C

A

C

C

A

A

G

A

L

K

A

A

T

V

L

L

R

-

T

S

L

V

V

V

D

D

H

A

I

F

V

C

D

A

E

E

V

V

E

A

E

R

K

K

G

G

Q

E

L

L

F

F

K

K

Q

E

L

L

Q

A

H

K

-

V

P

D

A

G

I

V

K

V

E

D

I

V

R

R

G

G

K

R

G

G

L

L

M

M

L

L

A

G

V

V

E

V

F

L

E

E

N

-

F

-

E

-

I

-

N

-

K

R

K

D

I

V

I

A

D

K

A

Q

C

A

I

V

L

L

D

D

G

G

V

F

L

K

S

H

D

G

W

V

F

I

L

L

H

N

-

A

-

P

C

A

S

D

N

N

S

I

M

I

R

R

I

L

A

T

P

P

L

L

I

V

I

I

T

T

K

D

E

E

I

I

A

A

E

D

A

A

C

V

T

K

I

A

I

I

L

A

K

E

N

T

V

I

binding pyridoxal-5'-phosphate: S108 (= S87), G109 (= G88), A110 (≠ T89), F135 (≠ Y115), H136 (= H116), E190 (= E166), D223 (= D199), V225 (≠ I201), Q226 (= Q202), K252 (= K228)

O66442 Acetylornithine aminotransferase; ACOAT; EC 2.6.1.11 from Aquifex aeolicus (strain VF5)
36% identity, 97% coverage: 2:367/378 of query aligns to 13:372/376 of O66442

query
sites
O66442

L

V

E

K

F

F

V

V

R

R

A

G

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K

G

G

I

V

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Y

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Y

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D

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D

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N

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G

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H

C

A

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Y

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P

A

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K

E

E

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Q

A

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Y

L

M

L

H

H

L

-

M

-

V

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S

G

N

E

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Y

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-

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F

F

F

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A

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N

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T

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GT 96:97 (= GT 88:89) binding pyridoxal 5'-phosphate
K242 (= K228) modified: N6-(pyridoxal phosphate)lysine
T271 (= T260) binding pyridoxal 5'-phosphate

2eh6A Crystal structure of acetylornithine aminotransferase from aquifex aeolicus vf5
36% identity, 97% coverage: 2:367/378 of query aligns to 12:371/375 of 2eh6A

query
sites
2eh6A

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x
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E

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x
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T

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active site: F127 (≠ Y115), E179 (= E166), D212 (= D199), Q215 (= Q202), K241 (= K228), T270 (= T260), R352 (= R348)
binding pyridoxal-5'-phosphate: G95 (= G88), T96 (= T89), F127 (≠ Y115), H128 (= H116), E179 (= E166), D212 (= D199), V214 (≠ I201), K241 (= K228)

Q9X2A5 Acetylornithine aminotransferase; ACOAT; EC 2.6.1.11 from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8)
36% identity, 97% coverage: 4:369/378 of query aligns to 14:380/385 of Q9X2A5

query
sites
Q9X2A5

F

F

V

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G