SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing CA265_RS15670 CA265_RS15670 4-hydroxy-tetrahydrodipicolinate reductase to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q9X1K8 4-hydroxy-tetrahydrodipicolinate reductase; HTPA reductase; EC 1.17.1.8 from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) (see paper)
34% identity, 99% coverage: 1:242/244 of query aligns to 1:211/216 of Q9X1K8

query
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Q9X1K8

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SGRM 9:12 (≠ -GKM 9:11) binding
GTT 71:73 (= GTT 75:77) binding
AY--NF 95:98 (≠ LYGSNF 98:103) binding
K131 (= K139) binding

1vm6B Crystal structure of dihydrodipicolinate reductase (tm1520) from thermotoga maritima at 2.27 a resolution
34% identity, 99% coverage: 1:242/244 of query aligns to 6:216/218 of 1vm6B

query
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1vm6B

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active site: H132 (= H135), K136 (= K139)
binding nicotinamide-adenine-dinucleotide: G12 (= G7), S14 (vs. gap), G15 (= G9), R16 (≠ K10), M17 (= M11), D37 (≠ T31), V38 (≠ I32), F53 (= F52), S54 (= S53), S55 (≠ T54), E57 (≠ D56), A58 (≠ S57), G76 (= G75), T78 (= T77), Y101 (= Y99), N102 (= N102), F103 (= F103), F192 (= F219)

4ywjA Crystal structure of 4-hydroxy-tetrahydrodipicolinate reductase (htpa reductase) from pseudomonas aeruginosa
35% identity, 81% coverage: 47:243/244 of query aligns to 71:265/268 of 4ywjA

query
sites
4ywjA

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active site: H156 (= H135), K160 (= K139)
binding nicotinamide-adenine-dinucleotide: F76 (= F52), T77 (≠ S53), V81 (≠ S57), G99 (= G75), T101 (= T77), A124 (≠ S101), N125 (= N102), F126 (= F103), R237 (= R216), F240 (= F219)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 11, 12, 13, 35, 36

5temA Structure of 4-hydroxy-tetrahydrodipicolinate reductase from vibrio vulnificus with 2,6 pyridine dicarboxylic and nadh (see paper)
35% identity, 85% coverage: 36:243/244 of query aligns to 60:264/266 of 5temA

query
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5temA

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active site: H155 (= H135), K159 (= K139)
binding nicotinamide-adenine-dinucleotide: F75 (= F52), T76 (≠ S53), S80 (= S57), G98 (= G75), T100 (= T77), P123 (≠ S101), N124 (= N102), Y125 (≠ F103), F239 (= F219)
binding pyridine-2,6-dicarboxylic acid: T100 (= T77), P123 (≠ S101), H156 (= H136), K159 (= K139), S164 (= S144), G165 (= G145), T166 (= T146)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 8, 11, 12, 13, 34, 35

5tejB Structure of 4-hydroxy-tetrahydrodipicolinate reductase from vibrio vulnificus with 2,5 furan dicarboxylic and nadh (see paper)
35% identity, 85% coverage: 36:243/244 of query aligns to 60:264/269 of 5tejB

query
sites
5tejB

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active site: H155 (= H135), K159 (= K139)
binding 2,5 Furan Dicarboxylic Acid: T100 (= T77), H156 (= H136), K159 (= K139), S164 (= S144), G165 (= G145), T166 (= T146)
binding nicotinamide-adenine-dinucleotide: F75 (= F52), T76 (≠ S53), S80 (= S57), G98 (= G75), T100 (= T77), P123 (≠ S101), N124 (= N102), Y125 (≠ F103), F239 (= F219)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 8, 11, 12, 13, 34, 35

5tejA Structure of 4-hydroxy-tetrahydrodipicolinate reductase from vibrio vulnificus with 2,5 furan dicarboxylic and nadh (see paper)
35% identity, 85% coverage: 36:243/244 of query aligns to 60:264/269 of 5tejA

query
sites
5tejA

E

D

D

D

L

L

T

A

R

K

Q

Q

N

-

L

I

K

D

S

Q

A

F

D

D

V

V

A

I

I

I

D

D

F
|
F

S
x
T

T

A

P

P

D

A

S
|
S

V

T

L

L

K

N

N

N

I

L

D

A

A

L

C

C

F

Q

D

Q

A

Y

N

G

V

K

P

S

I

I

V

V

V

I

G
|
G

T

T

T
|
T

G

G

W

F

Y

T

G

E

K

E

L

Q

Q

R

E

E

V

-

K

Q

D

I

D

D

C

L

N

V

N

A

S

Q

N

Q

N

V

T

P

L

V

Y

M

G

A

S
x
P

N

N

F

Y

S

S

I

V

G

G

V

V

N

N

L

L

F

V

F

F

K

K

L

L

N

L

Q

E

T

K

L

A

A

A

K

K

L

V

M

M

N

G

N

D

Y

Y

P

-

A

-

Y

C

E

D

V

I

Q

E

V

I

E

V

E

E

I

A

H
|
H

H

H

T

R

Q

H

K
|
K

L

V

D

D

A

A

P

P

S

S

G

G

T

T

A

A

I

I

T

G

L

M

A

G

E

E

G

A

I

I

V

A

D

G

N

A

L

M

D

G

R

N

K

K

Q

L

E

-

W

-

L

-

N

-

E

-

V

-

G

-

T

S

D

D

V

V

E

A

L

V

F

Y

P

A

K

R

-

E

-

G

-

I

-

T

-

G

-

E

-

R

-

T

A

K

E

D

Q

E

L

I

L

G

I

F

E

A

S

T

H

I

R

R

I

A

E

G

N

D

I

I

P

V

G

G

T

E

H

H

T

T

V

A

I

M

Y

F

S

A

S

D

E

I

V

G

D

E

E

R

I

V

E

E

I

I

K

T

H

H

T

K

A

A

H

T

N

D

R

R

A

M

G

T

F

F

A

A

L

N

G

G

A

A

V

V

V

K

A

A

E

V

W

W

L

L

K

H

D

E

K

K

-

P

K

A

G

G

F

F

F

Y

S

T

I

M

T

T

D

D

I

V

F

L

active site: H155 (= H135), K159 (= K139)
binding nicotinamide-adenine-dinucleotide: F75 (= F52), T76 (≠ S53), S80 (= S57), G98 (= G75), T100 (= T77), P123 (≠ S101)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 8, 11, 12, 13, 34, 35

1drvA Escherichia coli dhpr/acnadh complex (see paper)
30% identity, 88% coverage: 30:244/244 of query aligns to 57:266/270 of 1drvA

query
sites
1drvA

I

V

T

T

I

V

D

Q

N

S

Q

S

E

L

D

D

L

A

T

V

R

K

Q

D

N

D

L

F

K

-

S

-

A

-

D

D

V

V

A

F

I

I

D

D

F
|
F

S
x
T

T
x
R

P

P

D

E

S
x
G

V

T

L

L

K

N

N

H

I

L

D

A

A

F

C

C

F

R

D

Q

A

H

N

G

V

K

P

G

I

M

V

V

V

I

G
|
G

T

T

G

G

W

-

Y

F

G

D

K

E

L

A

Q

G

E

K

V

Q

K

A

D

I

D

R

C

D

N

A

S

A

N

D

N

I

T

A

L

I

L

V

Y

F

G

A

S
x
A

N

N

F
|
F

S

S

I

V

G

G

V

V

N

N

L

V

F

M

F

L

K

K

L

L

N

L

Q

E

T

K

L

A

A

A

K

K

L

V

M

M

N

G

N

D

Y

Y

P

T

A

-

Y

-

E

D

V

I

Q

E

V

I

E

I

E

E

I

A

H
|
H

H

H

T

R

Q

H

K
|
K

L

V

D

D

A

A

P

P

S

S

G

G

T

T

A

A

I

L

T

A

L

M

A

G

E

E

G

A

I

I

V

A

D

H

N

A

L

L

D

D

R

K

K

D

Q

L

E

K

W

-

L

-

N

-

E

-

V

-

G

-

T

-

D

D

V

C

E

A

L

V

F

Y

P

S

K

R

A

-

E

-

Q

-

L

-

I

-

E

E

S

G

H

H

R

T

I

G

E

E

N

R

I

V

P

P

G

G

T

T

-

I

-

G

-

F

-

A

-

T

-

V

-

R

-

A

-

G

-

D

-

I

-

V

-

G

-

E

H

H

T

T

V

A

I

M

Y

F

S

A

S

D

E

I

V

G

D

E

E

R

I

L

E

E

I

I

K

T

H

H

T

K

A

A

H

S

N

S

R
|
R

A

M

G

T

F

F

A

A

L

N

G

G

A

A

V

V

V

R

A

S

A

A

E

L

W

W

L

L

K

S

D

G

K

K

K

E

-

S

G

G

F

L

F

F

S

D

I

M

T

R

D

D

I

V

F

L

E

D

active site: H156 (= H135), K160 (= K139)
binding 3-acetylpyridine adenine dinucleotide: F76 (= F52), T77 (≠ S53), R78 (≠ T54), G81 (≠ S57), G99 (= G75), A124 (≠ S101), F126 (= F103), R237 (= R216)

Sites not aligning to the query:

binding 3-acetylpyridine adenine dinucleotide: 9, 12, 13, 14, 35

1druA Escherichia coli dhpr/nadh complex (see paper)
30% identity, 88% coverage: 30:244/244 of query aligns to 57:266/270 of 1druA

query
sites
1druA

I

V

T

T

I

V

D

Q

N

S

Q

S

E

L

D

D

L

A

T

V

R

K

Q

D

N

D

L

F

K

-

S

-

A

-

D

D

V

V

A

F

I

I

D

D

F
|
F

S
x
T

T
x
R

P

P

D

E

S
x
G

V

T

L

L

K

N

N

H

I

L

D

A

A

F

C

C

F

R

D

Q

A

H

N

G

V

K

P

G

I

M

V

V

V

I

G
|
G

T
|
T

G

G

W

-

Y

F

G

D

K

E

L

A

Q

G

E

K

V

Q

K

A

D

I

D

R

C

D

N

A

S

A

N

D

N

I

T

A

L

I

L

V

Y

F

G

A

S
x
A

N
|
N

F
|
F

S

S

I

V

G

G

V

V

N

N

L

V

F

M

F

L

K

K

L

L

N

L

Q

E

T

K

L

A

A

A

K

K

L

V

M

M

N

G

N

D

Y

Y

P

T

A

-

Y

-

E

D

V

I

Q

E

V

I

E

I

E

E

I

A

H
|
H

H

H

T

R

Q

H

K
|
K

L

V

D

D

A

A

P

P

S

S

G

G

T

T

A

A

I

L

T

A

L

M

A

G

E

E

G

A

I

I

V

A

D

H

N

A

L

L

D

D

R

K

K

D

Q

L

E

K

W

-

L

-

N

-

E

-

V

-

G

-

T

-

D

D

V

C

E

A

L

V

F

Y

P

S

K

R

A

-

E

-

Q

-

L

-

I

-

E

E

S

G

H

H

R

T

I

G

E

E

N

R

I

V

P

P

G

G

T

T

-

I

-

G

-

F

-

A

-

T

-

V

-

R

-

A

-

G

-

D

-

I

-

V

-

G

-

E

H

H

T

T

V

A

I

M

Y

F

S

A

S

D

E

I

V

G

D

E

E

R

I

L

E

E

I

I

K

T

H

H

T

K

A

A

H

S

N

S

R

R

A

M

G

T

F
|
F

A

A

L

N

G

G

A

A

V

V

V

R

A

S

A

A

E

L

W

W

L

L

K

S

D

G

K

K

K

E

-

S

G

G

F

L

F

F

S

D

I

M

T

R

D

D

I

V

F

L

E

D

active site: H156 (= H135), K160 (= K139)
binding nicotinamide-adenine-dinucleotide: F76 (= F52), T77 (≠ S53), R78 (≠ T54), G81 (≠ S57), G99 (= G75), T100 (= T76), T101 (= T77), A124 (≠ S101), N125 (= N102), F126 (= F103), F240 (= F219)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 9, 12, 13, 14, 35, 36

1arzA Escherichia coli dihydrodipicolinate reductase in complex with nadh and 2,6 pyridine dicarboxylate (see paper)
30% identity, 88% coverage: 30:244/244 of query aligns to 57:266/270 of 1arzA

query
sites
1arzA

I

V

T

T

I

V

D

Q

N

S

Q

S

E

L

D

D

L

A

T

V

R

K

Q

D

N

D

L

F

K

-

S

-

A

-

D

D

V

V

A

F

I

I

D

D

F

F

S

T

T

R

P

P

D

E

S

G

V

T

L

L

K

N

N

H

I

L

D

A

A

F

C

C

F

R

D

Q

A

H

N

G

V

K

P

G

I

M

V

V

V

I

G

G

T

T

G

G

W

-

Y

F

G

D

K

E

L

A

Q

G

E

K

V

Q

K

A

D

I

D

R

C

D

N

A

S

A

N

D

N

I

T

A

L

I

L

V

Y

F

G

A

S

A

N

N

F

F

S

S

I

V

G

G

V

V

N

N

L

V

F

M

F

L

K

K

L

L

N

L

Q

E

T

K

L

A

A

A

K

K

L

V

M

M

N

G

N

D

Y

Y

P

T

A

-

Y

-

E

D

V

I

Q

E

V

I

E

I

E

E

I

A

H
|
H

T

R

Q

H

K
|
K

L

V

D

D

A

A

P

P

S

S

G

G

T

T

A

A

I

L

T

A

L

M

A

G

E

E

G

A

I

I

V

A

D

H

N

A

L

L

D

D

R

K

K

D

Q

L

E

K

W

-

L

-

N

-

E

-

V

-

G

-

T

-

D

D

V

C

E

A

L

V

F

Y

P

S

K

R

A

-

E

-

Q

-

L

-

I

-

E

E

S

G

H

H

R

T

I

G

E

E

N

R

I

V

P

P

G

G

T

T

-

I

-

G

-

F

-

A

-

T

-

V

-

R

-

A

-

G

-

D

-

I

-

V

-

G

-

E

H

H

T

T

V

A

I

M

Y

F

S

A

S

D

E

I

V

G

D

E

E

R

I

L

E

E

I

I

K

T

H

H

T

K

A

A

H

S

N

S

R

R

A

M

G

T

F

F

A

A

L

N

G

G

A

A

V

V

V

R

A

S

A

A

E

L

W

W

L

L

K

S

D

G

K

K

K

E

-

S

G

G

F

L

F

F

S

D

I

M

T

R

D

D

I

V

F

L

E

D

active site: H156 (= H135), K160 (= K139)
binding phosphate ion: H157 (= H136), K160 (= K139)

1arzB Escherichia coli dihydrodipicolinate reductase in complex with nadh and 2,6 pyridine dicarboxylate (see paper)
30% identity, 88% coverage: 30:244/244 of query aligns to 56:265/269 of 1arzB

query
sites
1arzB

I

V

T

T

I

V

D

Q

N

S

Q

S

E

L

D

D

L

A

T

V

R

K

Q

D

N

D

L

F

K

-

S

-

A

-

D

D

V

V

A

F

I

I

D

D

F
|
F

S
x
T

T
x
R

P

P

D

E

S
x
G

V

T

L

L

K

N

N
x
H

I

L

D

A

A

F

C

C

F

R

D

Q

A

H

N

G

V

K

P

G

I

M

V

V

V

I

G
|
G

T

T

T
|
T

G

G

W

-

Y

F

G

D

K

E

L

A

Q

G

E

K

V

Q

K

A

D

I

D

R

C

D

N

A

S

A

N

D

N

I

T

A

L

I

L

V

Y

F

G

A

S
x
A

N
|
N

F
|
F

S

S

I

V

G

G

V

V

N

N

L

V

F

M

F

L

K

K

L

L

N

L

Q

E

T

K

L

A

A

A

K

K

L

V

M

M

N

G

N

D

Y

Y

P

T

A

-

Y

-

E

D

V

I

Q

E

V

I

E

I

E

E

I

A

H
|
H

T

R

Q

H

K
|
K

L

V

D

D

A

A

P

P

S
|
S

G
|
G

T
|
T

A

A

I

L

T

A

L

M

A

G

E

E

G

A

I

I

V

A

D

H

N

A

L

L

D

D

R

K

K

D

Q

L

E

K

W

-

L

-

N

-

E

-

V

-

G

-

T

-

D

D

V

C

E

A

L

V

F

Y

P

S

K

R

A

-

E

-

Q

-

L

-

I

-

E

E

S

G

H

H

R

T

I

G

E

E

N

R

I

V

P

P

G

G

T

T

-

I

-

G

-

F

-

A

-

T

-

V

-

R

-

A

-

G

-

D

-

I

-

V

-

G

-

E

H

H

T

T

V

A

I

M

Y

F

S

A

S

D

E

I

V

G

D

E

E

R

I

L

E

E

I

I

K

T

H

H

T

K

A

A

H

S

N

S

R

R

A

M

G

T

F
|
F

A

A

L

N

G

G

A

A

V

V

V

R

A

S

A

A

E

L

W

W

L

L

K

S

D

G

K

K

K

E

-

S

G

G

F

L

F

F

S

D

I

M

T

R

D

D

I

V

F

L

E

D

active site: H155 (= H135), K159 (= K139)
binding 1,4-dihydronicotinamide adenine dinucleotide: F75 (= F52), T76 (≠ S53), R77 (≠ T54), G80 (≠ S57), H84 (≠ N61), G98 (= G75), T100 (= T77), A123 (≠ S101), N124 (= N102), F125 (= F103), F239 (= F219)
binding pyridine-2,6-dicarboxylic acid: T100 (= T77), H156 (= H136), K159 (= K139), S164 (= S144), G165 (= G145), T166 (= T146), F239 (= F219)

Sites not aligning to the query:

binding 1,4-dihydronicotinamide adenine dinucleotide: 8, 10, 11, 12, 13, 34

P04036 4-hydroxy-tetrahydrodipicolinate reductase; HTPA reductase; EC 1.17.1.8 from Escherichia coli (strain K12) (see 3 papers)
30% identity, 88% coverage: 30:244/244 of query aligns to 60:269/273 of P04036

query
sites
P04036

I

V

T

T

I

V

D

Q

N

S

Q

S

E

L

D

D

L

A

T

V

R

K

Q

D

N

D

L

F

K

-

S

-

A

-

D

D

V

V

A

F

I

I

D

D

F

F

S
x
T

T
x
R

P

P

D

E

S

G

V

T

L

L

K

N

N

H

I

L

D

A

A

F

C

C

F

R

D

Q

A

H

N

G

V

K

P

G

I

M

V

V

V

I

G
|
G

T
|
T

G

G

W

-

Y

F

G

D

K

E

L

A

Q

G

E

K

V

Q

K

A

D

I

D

R

C

D

N

A

S

A

N

D

N

I

T

A

L

I

L

V

Y

F

G
x
A

S
x
A

N
|
N

F
|
F

S

S

I

V

G

G

V

V

N

N

L

V

F

M

F

L

K

K

L

L

N

L

Q

E

T

K

L

A

A

A

K

K

L

V

M

M

N

G

N

D

Y

Y

P

T

A

-

Y

-

E

D

V

I

Q

E

V

I

E

I

E

E

I

A

H
|
H

H

H

T

R

Q

H

K
|
K

L

V

D

D

A

A

P

P

S

S

G

G

T

T

A

A

I

L

T

A

L

M

A

G

E

E

G

A

I

I

V

A

D

H

N

A

L

L

D

D

R

K

K

D

Q

L

E

K

W

-

L

-

N

-

E

-

V

-

G

-

T

-

D

D

V

C

E

A

L

V

F

Y

P

S

K

R

A

-

E

-

Q

-

L

-

I

-

E

E

S

G

H

H

R

T

I

G

E

E

N

R

I

V

P

P

G

G

T

T

-

I

-

G

-

F

-

A

-

T

-

V

-

R

-

A

-

G

-

D

-

I

-

V

-

G

-

E

H

H

T

T

V

A

I

M

Y

F

S

A

S

D

E

I

V

G

D

E

E

R

I

L

E

E

I

I

K

T

H

H

T

K

A

A

H

S

N

S

R
|
R

A

M

G

T

F
|
F

A

A

L

N

G

G

A

A

V

V

V

R

A

S

A

A

E

L

W

W

L

L

K

S

D

G

K

K

K

E

-

S

G

G

F

L

F

F

S

D

I

M

T

R

D

D

I

V

F

L

E

D

TR 80:81 (≠ ST 53:54) binding
GTT 102:104 (= GTT 75:77) binding ; binding
AANF 126:129 (≠ GSNF 100:103) binding
F129 (= F103) binding
H159 (= H135) mutation H->A,Q: 135 to 200-fold reduction in catalytic activity.
K163 (= K139) binding ; mutation K->A,C,Q: 625 to 830-fold reduction in catalytic activity.
R240 (= R216) binding
F243 (= F219) binding

Sites not aligning to the query:

12 binding
15:17 binding
16:17 binding
38 binding
39 binding

1drwA Escherichia coli dhpr/nhdh complex (see paper)
30% identity, 88% coverage: 30:244/244 of query aligns to 59:268/272 of 1drwA

query
sites
1drwA

I

V

T

T

I

V

D

Q

N

S

Q

S

E

L

D

D

L

A

T

V

R

K

Q

D

N

D

L

F

K

-

S

-

A

-

D

D

V

V

A

F

I

I

D

D

F
|
F

S
x
T

T
x
R

P

P

D

E

S

G

V

T

L

L

K

N

N

H

I

L

D

A

A

F

C

C

F

R

D

Q

A

H

N

G

V

K

P

G

I

M

V

V

V

I

G
|
G

T
|
T

G

G

W

-

Y

F

G

D

K

E

L

A

Q

G

E

K

V

Q

K

A

D

I

D

R

C

D

N

A

S

A

N

D

N

I

T

A

L

I

L

V

Y

F

G

A

S
x
A

N
|
N

F
|
F

S

S

I

V

G

G

V

V

N

N

L

V

F

M

F

L

K

K

L

L

N

L

Q

E

T

K

L

A

A

A

K

K

L

V

M

M

N

G

N

D

Y

Y

P

T

A

-

Y

-

E

D

V

I

Q

E

V

I

E

I

E

E

I

A

H
|
H

H

H

T

R

Q

H

K
|
K

L

V

D

D

A

A

P

P

S

S

G

G

T

T

A

A

I

L

T

A

L

M

A

G

E

E

G

A

I

I

V

A

D

H

N

A

L

L

D

D

R

K

K

D

Q

L

E

K

W

-

L

-

N

-

E

-

V

-

G

-

T

-

D

D

V

C

E

A

L

V

F

Y

P

S

K

R

A

-

E

-

Q

-

L

-

I

-

E

E

S

G

H

H

R

T

I

G

E

E

N

R

I

V

P

P

G

G

T

T

-

I

-

G

-

F

-

A

-

T

-

V

-

R

-

A

-

G

-

D

-

I

-

V

-

G

-

E

H

H

T

T

V

A

I

M

Y

F

S

A

S

D

E

I

V

G

D

E

E

R

I

L

E

E

I

I

K

T

H

H

T

K

A

A

H

S

N

S

R

R

A

M

G

T

F
|
F

A

A

L

N

G

G

A

A

V

V

V

R

A

S

A

A

E

L

W

W

L

L

K

S

D

G

K

K

K

E

-

S

G

G

F

L

F

F

S

D

I

M

T

R

D

D

I

V

F

L

E

D

active site: H158 (= H135), K162 (= K139)
binding nicotinamide purin-6-ol-dinucleotide: F78 (= F52), T79 (≠ S53), R80 (≠ T54), G101 (= G75), T102 (= T76), T103 (= T77), A126 (≠ S101), N127 (= N102), F128 (= F103), F242 (= F219)

Sites not aligning to the query:

binding nicotinamide purin-6-ol-dinucleotide: 11, 14, 15, 16, 37, 38

1dihA Three-dimensional structure of e. Coli dihydrodipicolinate reductase (see paper)
30% identity, 88% coverage: 30:244/244 of query aligns to 59:268/272 of 1dihA

query
sites
1dihA

I

V

T

T

I

V

D

Q

N

S

Q

S

E

L

D

D

L

A

T

V

R

K

Q

D

N

D

L

F

K

-

S

-

A

-

D

D

V

V

A

F

I

I

D

D

F
|
F

S
x
T

T
x
R

P

P

D

E

S
x
G

V

T

L

L

K

N

N

H

I

L

D

A

A

F

C

C

F

R

D

Q

A

H

N

G

V

K

P

G

I

M

V

V

V

I

G
|
G

T

T

T
|
T

G

G

W

-

Y

F

G

D

K

E

L

A

Q

G

E

K

V

Q

K

A

D

I

D

R

C

D

N

A

S

A

N

D

N

I

T

A

L

I

L

V

Y

F

G

A

S

A

N
|
N

F
|
F

S

S

I

V

G

G

V

V

N

N

L

V

F

M

F

L

K

K

L

L

N

L

Q

E

T

K

L

A

A

A

K

K

L

V

M

M

N

G

N

D

Y

Y

P

T

A

-

Y

-

E

D

V

I

Q

E

V

I

E

I

E

E

I

A

H
|
H

H

H

T

R

Q

H

K
|
K

L

V

D

D

A

A

P

P

S

S

G

G

T

T

A

A

I

L

T

A

L

M

A

G

E

E

G

A

I

I

V

A

D

H

N

A

L

L

D

D

R

K

K

D

Q

L

E

K

W

-

L

-

N

-

E

-

V

-

G

-

T

-

D

D

V

C

E

A

L

V

F

Y

P

S

K

R

A

-

E

-

Q

-

L

-

I

-

E

E

S

G

H

H

R

T

I

G

E

E

N

R

I

V

P

P

G

G

T

T

-

I

-

G

-

F

-

A

-

T

-

V

-

R

-

A

-

G

-

D

-

I

-

V

-

G

-

E

H

H

T

T

V

A

I

M

Y

F

S

A

S

D

E

I

V

G

D

E

E

R

I

L

E

E

I

I

K

T

H

H

T

K

A

A

H

S

N

S

R
|
R

A

M

G

T

F
|
F

A

A

L

N

G

G

A

A

V

V

V

R

A

S

A

A

E

L

W

W

L

L

K

S

D

G

K

K

K

E

-

S

G

G

F

L

F

F

S

D

I

M

T

R

D

D

I

V

F

L

E

D

active site: H158 (= H135), K162 (= K139)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: F78 (= F52), T79 (≠ S53), R80 (≠ T54), G83 (≠ S57), G101 (= G75), T103 (= T77), N127 (= N102), F128 (= F103), R239 (= R216), F242 (= F219)

Sites not aligning to the query:

binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 11, 14, 15, 16, 38

5eesA Crystal structure of dapb in complex with NADP+ from corynebacterium glutamicum (see paper)
29% identity, 97% coverage: 1:236/244 of query aligns to 2:237/247 of 5eesA

query
sites
5eesA

M

I

K

K

L

V

A

G

L

V

L

L

G
|
G

Y

A

-

K

G
|
G

K
x
R

M
x
V

G

G

Q

Q

I

T

I

I

E

V

K

A

F

A

A

V

V

N

E

E

R

S

G

D

H

D

-

L

E

E

I

L

V

V

L

A

K

E

I

I

T
x
G

I
x
V

D

D

N

D

Q

D

E

L

D

S

L

L

T

L

R

V

Q

D

N

N

L

-

K

-

S

G

A

A

D

E

V

V

A

V

I

V

D

D

F
|
F

S
x
T

T

T

P

P

D

N

S

A

V

V

L

M

K

G

N

N

I

L

D

E

A

F

C

C

F

I

D

N

A

N

N

G

V

I

P

S

I

A

V

V

G
|
G

T

T

T
|
T

G

G

W

F

-

D

Y

D

G

A

K

R

L

L

Q

E

E

Q

V

V

K

R

D

D

D

W

C

L

N

E

N

G

S

K

N

D

N

N

T

V

-

G

L

V

L

L

Y

I

G

A

S
x
P

N
|
N

F
|
F

S

A

I

I

G

S

V

A

N

V

L

L

F

T

F

M

K

V

L

F

N

S

Q

K

T

Q

L

A

A

A

K

R

L

F

M

F

N

E

N

S

Y

-

P

-

A

-

Y

-

E

-

V

A

Q

E

V

V

E

I

E

E

I

L

H
|
H

T

P

Q

N

K
|
K

L

L

D

D

A

A

P

P

S

S

G
|
G

T
|
T

A

A

I

I

T

H

L

T

A

A

E

Q

G

G

I

I

V

A

-

A

-

R

-

K

-

E

-

A

-

G

-

M

-

D

D

A

N

Q

L

P

D

D

R

A

K

T

Q

E

E

Q

W

A

L

L

N

E

E

G

V

S

V

R

G

G

T

A

D

S

V

V

E

D

L

G

F

I

P

P

K

-

A

-

E

-

Q

-

L

-

I

V

E

H

S

A

H

V

R

R

I

M

E

S

N

G

I

M

P

V

G

A

T

H

H

E

T

Q

V

V

I

I

Y

F

S

G

S

T

E

Q

V

G

D

Q

E

T

I

L

E

T

I

I

K

K

H

Q

T

D

A

S

H

Y

N

D

R

R

A

N

G

S

F
|
F

A

A

L

P

G

G

A

V

V

L

V

V

A

G

A

V

E

R

W

N

L

I

K

A

D

Q

K

H

K

P

G

G

F

L

active site: H133 (= H135), K137 (= K139)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G7), G11 (= G9), R12 (≠ K10), V13 (≠ M11), G34 (≠ T31), V35 (≠ I32), F53 (= F52), T54 (≠ S53), G76 (= G75), T78 (= T77), P104 (≠ S101), N105 (= N102), F106 (= F103), F220 (= F219)
binding sulfate ion: H134 (= H136), K137 (= K139), K137 (= K139), G143 (= G145), T144 (= T146)

5eerA Crystal structure of dapb from corynebacterium glutamicum (see paper)
29% identity, 97% coverage: 1:236/244 of query aligns to 2:237/247 of 5eerA

query
sites
5eerA

M

I

K

K

L

V

A

G

L

V

L

L

G

G

Y

A

-

K

G

G

K

R

M

V

G

G

Q

Q

I

T

I

I

E

V

K

A

F

A

A

V

V

N

E

E

R

S

G

D

H

D

-

L

E

E

I

L

V

V

L

A

K

E

I

I

T

G

I

V

D

D

N

D

Q

D

E

L

D

S

L

L

T

L

R

V

Q

D

N

N

L

-

K

-

S

G

A

A

D

E

V

V

A

V

I

V

D

D

F

F

S

T

T

T

P

P

D

N

S

A

V

V

L

M

K

G

N

N

I

L

D

E

A

F

C

C

F

I

D

N

A

N

N

G

V

I

P

S

I

A

V

V

G

G

T

T

G

G

W

F

-

D

Y

D

G

A

K

R

L

L

Q

E

E

Q

V

V

K

R

D

D

D

W

C

L

N

E

N

G

S

K

N

D

N

N

T

V

-

G

L

V

L

L

Y

I

G

A

S

P

N

N

F

F

S

A

I

I

G

S

V

A

N

V

L

L

F

T

F

M

K

V

L

F

N

S

Q

K

T

Q

L

A

A

A

K

R

L

F

M

F

N

E

N

S

Y

-

P

-

A

-

Y

-

E

-

V

A

Q

E

V

V

E

I

E

E

I

L

H
|
H

T

P

Q

N

K
|
K

L

L

D

D

A

A

P

P

S
|
S

G
|
G

T
|
T

A

A

I

I

T

H

L

T

A

A

E

Q

G

G

I

I

V

A

-

A

-

R

-

K

-

E

-

A

-

G

-

M

-

D

D

A

N

Q

L

P

D

D

R

A

K

T

Q

E

E

Q

W

A

L

L

N

E

E

G

V

S

V

R

G

G

T

A

D

S

V

V

E

D

L

G

F

I

P

P

K

-

A

-

E

-

Q

-

L

-

I

V

E

H

S

A

H

V

R

R

I

M

E

S

N

G

I

M

P

V

G

A

T

H

H

E

T

Q

V

V

I

I

Y

F

S

G

S

T

E

Q

V

G

D

Q

E

T

I

L

E

T

I

I

K

K

H

Q

T

D

A

S

H

Y

N

D

R

R

A

N

G

S

F

F

A

A

L

P

G

G

A

V

V

L

V

V

A

G

A

V

E

R

W

N

L

I

K

A

D

Q

K

H

K

P

G

G

F

L

active site: H133 (= H135), K137 (= K139)
binding sulfate ion: H134 (= H136), K137 (= K139), K137 (= K139), S142 (= S144), G143 (= G145), T144 (= T146)

P9WP23 4-hydroxy-tetrahydrodipicolinate reductase; HTPA reductase; EC 1.17.1.8 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
27% identity, 97% coverage: 1:236/244 of query aligns to 1:234/245 of P9WP23

query
sites
P9WP23

M

M

K

R

L

V

A

G

L

V

L

L

G

G

Y

A

-
x
K

G

G

K
|
K

M
x
V

G

G

Q

A

I

T

I

M

E

V

K

R

F

-

A

A

V

V

E

A

R

A

G

A

H

D

E

D

I

L

V

T

L

L

K

S

I

A

T

E

I

L

D
|
D

N

A

Q

G

E

D

D

P

L

L

T

S

R

L

Q

L

N

T

L

D

K

G

S

N

A

T

D

E

V

V

A

V

I

I

D

D

F

F

S

T

T

H

P

P

D

D

S

V

V

V

L

M

K

G

N

N

I

L

D

E

A

F

C

L

F

I

D

D

A

N

N

G

V

I

P

H

I

A

V

V

G
|
G

T
|
T

G

G

W

F

Y

T

G

A

-

E

K

R

L

F

Q

Q

E

Q

V

V

K

E

D

S

-

W

D

L

C

V

N

A

N

K

S

P

N

N

N

T

T

S

L

V

L

L

Y

I

G
x
A

S
x
P

N
|
N

F
|
F

S

A

I

I

G

G

V

A

N

V

L

L

F

S

F

M

K

H

L

F

N

A

Q

K

T

Q

L

A

A

A

K

R

L

F

M

F

N

D

N

S

Y

-

P

-

A

-

Y

-

E

-

V

A

Q

E

V

V

E

I

E

E

I

L

H

H

T

P

Q

H

K
|
K

L

A

D

D

A

A

P

P

S

S

G

G

T

T

A

A

I

A

T

R

L

T

A

A

-

K

-

L

-

I

-

A

-

E

-

A

-

R

E

K

G

G

I

L

V

P

D

P

N

N

L

P

D

D

R

A

K

T

Q

S

E

T

W

S

L

L

N

P

E

G

V

A

V

R

G

G

T

A

D

D

V

V

E

D

L

G

F

I

P

P

K

-

A

-

E

-

Q

-

L

-

I

V

E

H

S

A

H

V

R

R

I

L

E

A

N

G

I

L

P

V

G

A

T

H

H

Q

T

E

V

V

I

L

Y

F

S

G

S

T

E

E

V

G

D

E

E

T

I

L

E

T

I

I

K

R

H

H

T

D

A

S

H

L

N

D

R

R

A

T

G

S

F

F

A

V

L

P

G

G

A

V

V

L

A

A

A

V

E

R

W

R

L

I

K

A

D

E

K

R

K

P

G

G

F

L

K9 (vs. gap) mutation to A: Increases the nucleotide specificity from 6:1 for the wild-type enzyme to 34:1, due to a 4-fold decrease in NADPH affinity while the affinity for NADH remains nearly unchanged.
K11 (= K10) mutation to A: 2.8-fold increase in catalytic activity with NADH as substrate, while the affinity for NADH is essentially unaffected. 70-fold decrease in affinity for NADPH, causing the nucleotide specificity to increase from 6:1 for the wild-type enzyme to 187:1.
KV 11:12 (≠ KM 10:11) binding ; binding
D33 (= D33) binding
GTT 75:77 (= GTT 75:77) binding ; binding
APNF 102:105 (≠ GSNF 100:103) binding ; binding
K136 (= K139) binding ; binding

5ugvA Dapb from mycobacterium tuberculosis (see paper)
27% identity, 97% coverage: 1:236/244 of query aligns to 2:235/245 of 5ugvA

query
sites
5ugvA

M

M

K

R

L

V

A

G

L

V

L

L

G
|
G

Y

A

-

K

G
|
G

K
|
K

M
x
V

G

G

Q

A

I

T

I

M

E

V

K

R

F

-

A

A

V

V

E

A

R

A

G

A

H

D

E

D

I

L

V

T

L

L

K

S

I

A

T

E

I

L

D
|
D

N
x
A

Q

G

E

D

D

P

L

L

T

S

R

L

Q

L

N

T

L

D

K

G

S

N

A

T

D

E

V

V

A

V

I

I

D

D

F
|
F

S
x
T

T

H

P

P

D

D

S
x
V

V

V

L

M

K

G

N

N

I

L

D

E

A

F

C

L

F

I

D

D

A

N

N

G

V

I

P

H

I

A

V

V

G

G

T

T

T
|
T

G

G

W

F

Y

T

G

A

-

E

K

R

L

F

Q

Q

E

Q

V

V

K

E

D

S

-

W

D

L

C

V

N

A

N

K

S

P

N

N

N

T

T

S

L

V

L

L

Y

I

G

A

S

P

N

N

F

F

S

A

I

I

G

G

V

A

N

V

L

L

F

S

F

M

K

H

L

F

N

A

Q

K

T

Q

L

A

A

A

K

R

L

F

M

F

N

D

N

S

Y

-

P

-

A

-

Y

-

E

-

V

A

Q

E

V

V

E

I

E

E

I

L

H

H

T

P

Q

H

K

K

L

A

D

D

A

A

P

P

S

S

G

G

T

T

A

A

I

A

T

R

L

T

A

A

-

K

-

L

-

I

-

A

-

E

-

A

-

R

E

K

G

G

I

L

V

P

D

P

N

N

L

P

D

D

R

A

K

T

Q

S

E

T

W

S

L

L

N

P

E

G

V

A

V

R

G

G

T

A

D

D

V

V

E

D

L

G

F

I

P

P

K

-

A

-

E

-

Q

-

L

-

I

V

E

H

S

A

H

V

R

R

I

L

E

A

N

G

I

L

P

V

G

A

T

H

H

Q

T

E

V

V

I

L

Y

F

S

G

S

T

E

E

V

G

D

E

E

T

I

L

E

T

I

I

K

R

H

H

T

D

A

S

H

L

N

D

R

R

A

T

G

S

F

F

A

V

L

P

G

G

A

V

V

L

A

A

A

V

E

R

W

R

L

I

K

A

D

E

K

R

K

P

G

G

F

L

binding nicotinamide-adenine-dinucleotide: G8 (= G7), G11 (= G9), K12 (= K10), V13 (≠ M11), D34 (= D33), A35 (≠ N34), F53 (= F52), T54 (≠ S53), V58 (≠ S57), T78 (= T77)

5tjzA Structure of 4-hydroxytetrahydrodipicolinate reductase from mycobacterium tuberculosis with NADPH and 2,6 pyridine dicarboxylic acid (see paper)
27% identity, 97% coverage: 1:236/244 of query aligns to 2:235/245 of 5tjzA

query
sites
5tjzA

M

M

K

R

L

V

A

G

L

V

L

L

G
|
G

Y

A

-

K

G
|
G

K
|
K

M
x
V

G

G

Q

A

I

T

I

M

E

V

K

R

F

-

A

A

V

V

E

A

R

A

G

A

H

D

E

D

I

L

V

T

L

L

K

S

I

A

T

E

I

L

D
|
D

N
x
A

Q

G

E

D

D

P

L

L

T

S

R

L

Q

L

N

T

L

D

K

G

S

N

A

T

D

E

V

V

A

V

I

I

D

D

F
|
F

S
x
T

T

H

P

P

D

D

S

V

V

V

L

M

K

G

N

N

I

L

D

E

A

F

C

L

F

I

D

D

A

N

N

G

V

I

P

H

I

A

V

V

G
|
G

T
|
T

G

G

W

F

Y

T

G

A

-

E

K

R

L

F

Q

Q

E

Q

V

V

K

E

D

S

-

W

D

L

C

V

N

A

N

K

S

P

N

N

N

T

T

S

L

V

L

L

Y

I

G

A

S
x
P

N
|
N

F
|
F

S

A

I

I

G

G

V

A

N

V

L

L

F

S

F

M

K

H

L

F

N

A

Q

K

T

Q

L

A

A

A

K

R

L

F

M

F

N

D

N

S

Y

-

P

-

A

-

Y

-

E

-

V

A

Q

E

V

V

E

I

E

E

I

L

H
|
H

T

P

Q

H

K
|
K

L

A

D

D

A

A

P

P

S
|
S

G
|
G

T
|
T

A

A

I

A

T

R

L

T

A

A

-

K

-

L

-

I

-

A

-

E

-

A

-

R

E

K

G

G

I

L

V

P

D

P

N

N

L

P

D

D

R

A

K

T

Q

S

E

T

W

S

L

L

N

P

E

G

V

A

V

R

G

G

T

A

D

D

V

V

E

D

L

G

F

I

P

P

K

-

A

-

E

-

Q

-

L

-

I

V

E

H

S

A

H

V

R

R

I

L

E

A

N

G

I

L

P

V

G
x
A

T

H

H

Q

T

E

V

V

I

L

Y

F

S

G

S

T

E

E

V

G

D

E

E

T

I

L

E

T

I

I

K

R

H

H

T

D

A

S

H

L

N

D

R

R

A

T

G

S

F
|
F

A

V

L

P

G

G

A

V

V

L

A

A

A

V

E

R

W

R

L

I

K

A

D

E

K

R

K

P

G

G

F

L

active site: H133 (= H135), K137 (= K139)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G7), G11 (= G9), K12 (= K10), V13 (≠ M11), D34 (= D33), A35 (≠ N34), F53 (= F52), T54 (≠ S53), G76 (= G75), T77 (= T76), T78 (= T77), P104 (≠ S101), N105 (= N102), F106 (= F103), F218 (= F219)
binding pyridine-2,6-dicarboxylic acid: T78 (= T77), P104 (≠ S101), H134 (= H136), K137 (= K139), S142 (= S144), G143 (= G145), T144 (= T146), A193 (≠ G194)

1p9lA Structure of m. Tuberculosis dihydrodipicolinate reductase in complex with nadh and 2,6 pdc (see paper)
27% identity, 97% coverage: 1:236/244 of query aligns to 1:234/245 of 1p9lA

query
sites
1p9lA

M

M

K

R

L

V

A

G

L

V

L

L

G
|
G

Y

A

-

K

G
|
G

K
|
K

M
x
V

G

G

Q

T

I

T

I

M

E

V

K

R

F

-

A

A

V

V

E

A

R

A

G

A

H

D

E

D

I

L

V

T

L

L

K

S

I

A

T

E

I

L

D
|
D

N
x
A

Q

G

E

D

D

P

L

L

T

S

R

L

Q

L

N

T

L

D

K

G

S

N

A

T

D

E

V

V

A

V

I

I

D

D

F
|
F

S
x
T

T

H

P

P

D

D

S
x
V

V

V

L

M

K

G

N

N

I

L

D

E

A

F

C

L

F

I

D

D

A

N

N

G

V

I

P

H

I

A

V

V

G
|
G

T

T

T
|
T

G

G

W

F

Y

T

G

A

-

E

K

R

L

F

Q

Q

E

Q

V

V

K

E

D

S

-

W

D

L

C

V

N

A

N

K

S

P

N

N

N

T

T

S

L

V

L

L

Y

I

G

A

S
x
P

N
|
N

F
|
F

S

A

I

I

G

G

V

A

N

V

L

L

F

S

F

M

K

H

L

F

N

A

Q

K

T

Q

L

A

A

A

K

R

L

F

M

F

N

D

N

S

Y

-

P

-

A

-

Y

-

E

-

V

A

Q

E

V

V

E

I

E

E

I

L

H
|
H

T

P

Q

H

K
|
K

L

A

D

D

A

A

P

P

S
|
S

G
|
G

T
|
T

A

A

I

A

T

R

L

T

A

A

-

K

-

L

-

I

-

A

-

E

-

A

-

R

E

K

G

G

I

L

V

P

D

P

N

N

L

P

D

D

R

A

K

T

Q

S

E

T

W

S

L

L

N

P

E

G

V

A

V

R

G

G

T

A

D

D

V

V

E

D

L

G

F

I

P

P

K

-

A

-

E

-

Q

-

L

-

I

V

E

H

S

A

H

V

R

R

I

L

E

A

N

G

I

L

P

V

G
x
A

T

H

H

Q

T

E

V

V

I

L

Y

F

S

G

S

T

E

E

V

G

D

E

E

T

I

L

E

T

I

I

K

R

H

H

T

D

A

S

H

L

N

D

R

R

A

T

G

S

F
|
F

A

V

L

P

G

G

A

V

V

L

A

A

A

V

E

R

W

R

L

I

K

A

D

E

K

R

K

P

G

G

F

L

active site: H132 (= H135), K136 (= K139)
binding 1,4-dihydronicotinamide adenine dinucleotide: G7 (= G7), G10 (= G9), K11 (= K10), V12 (≠ M11), D33 (= D33), A34 (≠ N34), F52 (= F52), T53 (≠ S53), V57 (≠ S57), G75 (= G75), T77 (= T77), P103 (≠ S101), N104 (= N102), F105 (= F103), F217 (= F219)
binding pyridine-2,6-dicarboxylic acid: H133 (= H136), K136 (= K139), S141 (= S144), G142 (= G145), T143 (= T146), A192 (≠ G194)

1c3vA Dihydrodipicolinate reductase from mycobacterium tuberculosis complexed with NADPH and pdc (see paper)
27% identity, 97% coverage: 1:236/244 of query aligns to 1:234/245 of 1c3vA

query
sites
1c3vA

M

M

K

R

L

V

A

G

L

V

L

L

G

G

Y

A

-
x
K

G
|
G

K
|
K

M
x
V

G

G

Q

T

I

T

I

M

E

V

K

R

F

-

A

A

V

V

E

A

R

A

G

A

H

D

E

D

I

L

V

T

L

L

K

S

I

A

T

E

I

L

D
|
D

N
x
A

Q

G

E

D

D

P

L

L

T

S

R

L

Q

L

N

T

L

D

K

G

S

N

A

T

D

E

V

V

A

V

I

I

D

D

F
|
F

S
x
T

T

H

P

P

D

D

S
x
V

V

V

L

M

K

G

N

N

I

L

D

E

A

F

C

L

F

I

D

D

A

N

N

G

V

I

P

H

I

A

V

V

G
|
G

T

T

T
|
T

G

G

W

F

Y

T

G

A

-

E

K

R

L

F

Q

Q

E

Q

V

V

K

E

D

S

-

W

D

L

C

V

N

A

N

K

S

P

N

N

N

T

T

S

L

V

L

L

Y

I

G

A

S
x
P

N
|
N

F

F

S

A

I

I

G

G

V

A

N

V

L

L

F

S

F

M

K

H

L

F

N

A

Q

K

T

Q

L

A

A

A

K

R

L

F

M

F

N

D

N

S

Y

-

P

-

A

-

Y

-

E

-

V

A

Q

E

V

V

E

I

E

E

I

L

H
|
H

H

H

T

P

Q

H

K
|
K

L

A

D

D

A

A

P

P

S
|
S

G
|
G

T
|
T

A

A

I

A

T

R

L

T

A

A

-

K

-

L

-

I

-

A

-

E

-

A

-

R

E

K

G

G

I

L

V

P

D

P

N

N

L

P

D

D

R

A

K

T

Q

S

E

T

W

S

L

L

N

P

E

G

V

A

V

R

G

G

T

A

D

D

V

V

E

D

L

G

F

I

P

P

K

-

A

-

E

-

Q

-

L

-

I

V

E

H

S

A

H

V

R

R

I

L

E

A

N

G

I

L

P

V

G
x
A

T

H

H

Q

T

E

V

V

I

L

Y

F

S

G

S

T

E

E

V

G

D

E

E

T

I

L

E

T

I

I

K

R

H

H

T

D

A

S

H

L

N

D

R

R

A

T

G

S

F
|
F

A

V

L

P

G

G

A

V

V

L

A

A

A

V

E

R

W

R

L

I

K

A

D

E

K

R

K

P

G

G

F

L

active site: H132 (= H135), K136 (= K139)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: K9 (vs. gap), G10 (= G9), K11 (= K10), V12 (≠ M11), D33 (= D33), A34 (≠ N34), F52 (= F52), T53 (≠ S53), V57 (≠ S57), G75 (= G75), T77 (= T77), P103 (≠ S101), N104 (= N102), F217 (= F219)
binding pyridine-2,6-dicarboxylic acid: T77 (= T77), N104 (= N102), K136 (= K139), S141 (= S144), G142 (= G145), T143 (= T146), A192 (≠ G194)

Query Sequence

>CA265_RS15670 CA265_RS15670 4-hydroxy-tetrahydrodipicolinate reductase
MKLALLGYGKMGQIIEKFAVERGHEIVLKITIDNQEDLTRQNLKSADVAIDFSTPDSVLK
NIDACFDANVPIVVGTTGWYGKLQEVKDDCNNSNNTLLYGSNFSIGVNLFFKLNQTLAKL
MNNYPAYEVQVEEIHHTQKLDAPSGTAITLAEGIVDNLDRKQEWLNEVVGTDVELFPKAE
QLLIESHRIENIPGTHTVIYSSEVDEIEIKHTAHNRAGFALGAVVAAEWLKDKKGFFSIT
DIFE

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory