SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing CA265_RS15805 FitnessBrowser__Pedo557:CA265_RS15805 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P09114 Acetolactate synthase 2, chloroplastic; ALS II; Acetohydroxy-acid synthase II; Acetolactate synthase II; EC 2.2.1.6 from Nicotiana tabacum (Common tobacco) (see paper)
44% identity, 97% coverage: 14:580/583 of query aligns to 77:654/664 of P09114

query
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P09114

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P191 (≠ F125) mutation to A: In S4-Hra; highly resistant to sulfonylurea herbicides; when associated with L-568.
W568 (= W502) mutation to L: In S4-Hra; highly resistant to sulfonylurea herbicides; when associated with A-191.

P09342 Acetolactate synthase 1, chloroplastic; ALS I; Acetohydroxy-acid synthase I; Acetolactate synthase I; EC 2.2.1.6 from Nicotiana tabacum (Common tobacco) (see 2 papers)
43% identity, 99% coverage: 3:580/583 of query aligns to 64:657/667 of P09342

query
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P09342

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C161 (≠ V92) modified: Disulfide link with 307
P194 (≠ F125) mutation to Q: In C3; highly resistant to sulfonylurea herbicides.
C307 (≠ V237) modified: Disulfide link with 161

7tzzA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase p197t mutant in complex with bispyribac-sodium (see paper)
42% identity, 95% coverage: 26:580/583 of query aligns to 14:575/582 of 7tzzA

query
sites
7tzzA

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binding 2,6-bis[(4,6-dimethoxypyrimidin-2-yl)oxy]benzoic acid: M266 (= M280), R292 (= R306), W489 (= W502), S568 (≠ Q573)
binding 2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-2-[(1~{S})-1-(dioxidanyl)-1-oxidanyl-ethyl]-4-methyl-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: V400 (= V413), G401 (= G414), Q402 (= Q415), H403 (= H416), G426 (= G439), M428 (= M441), G452 (= G465), D453 (= D466), G454 (= G467), S455 (≠ G468), L483 (= L496), G484 (= G497), M485 (= M498), V486 (= V499)
binding flavin-adenine dinucleotide: R161 (= R174), G222 (= G234), G223 (≠ Q235), G224 (= G236), T246 (= T260), L247 (≠ I261), M248 (= M262), M263 (= M277), L264 (= L278), M266 (= M280), H267 (= H281), G286 (= G300), R288 (= R302), V293 (= V307), D310 (= D324), I311 (= I325), D329 (= D343), V330 (≠ C344), M405 (= M418), G423 (= G436)
binding magnesium ion: A37 (= A49), T82 (= T95), S83 (= S96), Q122 (= Q135), Y381 (≠ E394), D453 (= D466), M458 (= M471), Q461 (= Q474), N480 (= N493), H482 (≠ F495), K533 (≠ R538)

P17597 Acetolactate synthase, chloroplastic; AtALS; Acetohydroxy-acid synthase; Protein CHLORSULFURON RESISTANT 1; EC 2.2.1.6 from Arabidopsis thaliana (Mouse-ear cress) (see 8 papers)
42% identity, 95% coverage: 26:580/583 of query aligns to 99:660/670 of P17597

query
sites
P17597

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A122 (= A49) mutation to V: Reduced catalytic activity. Resistant to imidazolinone herbicides but not to sulfonylurea herbicides.
M124 (= M51) mutation to E: Reduced catalytic activity. Resistant to imidazolinone herbicides and reduced sensitivity to sulfonylurea herbicides.; mutation to I: No effect on catalytic activity. Increased resistance to imidazolinone herbicides.
E144 (= E72) binding thiamine diphosphate
S186 (= S114) binding FAD
P197 (≠ F125) mutation to S: In csr1-1/GH50; resistant to sulfonylurea but not to imidazolinone herbicides.
R199 (≠ H127) mutation R->A,E: No effect on catalytic activity. Resistant to imidazolinone herbicides but not to sulfonylurea herbicides.
Q207 (= Q135) binding thiamine diphosphate
K220 (= K148) binding (R)-imazaquin
R246 (= R174) binding (R)-imazaquin; binding FAD
K256 (= K184) binding chlorimuron-ethyl
G308 (≠ Q235) binding FAD
TL 331:332 (≠ TI 260:261) binding FAD
C340 (≠ T269) modified: Cysteine sulfinic acid (-SO2H)
LGMH 349:352 (= LGMH 278:281) binding FAD
GVRFD 371:375 (≠ GMRFD 300:304) binding FAD
DR 376:377 (= DR 305:306) binding chlorimuron-ethyl
DI 395:396 (= DI 324:325) binding FAD
DV 414:415 (≠ DC 343:344) binding FAD
QH 487:488 (= QH 415:416) binding thiamine diphosphate
GG 508:509 (= GG 436:437) binding FAD
GAM 511:513 (≠ GTM 439:441) binding thiamine diphosphate
D538 (= D466) binding Mg(2+)
DGS 538:540 (≠ DGG 466:468) binding thiamine diphosphate
N565 (= N493) binding Mg(2+)
NQHLGM 565:570 (≠ NRFLGM 493:498) binding thiamine diphosphate
H567 (≠ F495) binding Mg(2+)
W574 (= W502) binding chlorimuron-ethyl; mutation to L: Increased catalytic activity. Resistant to imidazolinone and sulfonylurea herbicides.; mutation to S: Slightly decreased catalytic activity. Resistant to imidazolinone and sulfonylurea herbicides.
S653 (≠ Q573) binding chlorimuron-ethyl; mutation to A: No effect on catalytic activity or sensitivity to herbicides.; mutation to F: No effect on catalytic activity. Resistant to imidazolinone herbicides and also slightly sulfonylurea-resistant.; mutation to N: In csr1-2/GH90; no effect on catalytic activity. Resistant to imidazolinone but not to sulfonylurea herbicides.; mutation to T: No effect on catalytic activity. Resistant to imidazolinone herbicides but not to sulfonylurea herbicides.

5k2oA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a pyrimidinyl-benzoate herbicide, pyrithiobac (see paper)
42% identity, 95% coverage: 26:580/583 of query aligns to 14:575/585 of 5k2oA

query
sites
5k2oA

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active site: Y33 (= Y45), G35 (= G47), G36 (= G48), A37 (= A49), S38 (≠ I50), E59 (= E72), T82 (= T95), F121 (= F134), Q122 (= Q135), E123 (= E136), K171 (= K184), M266 (= M280), V293 (= V307), V400 (= V413), G426 (= G439), M428 (= M441), D453 (= D466), N480 (= N493), H482 (≠ F495), L483 (= L496), M485 (= M498), V486 (= V499), W489 (= W502), H558 (≠ R563)
binding 2-chloranyl-6-(4,6-dimethoxypyrimidin-2-yl)sulfanyl-benzoic acid: M266 (= M280), R292 (= R306), W489 (= W502), S568 (≠ Q573)
binding flavin-adenine dinucleotide: R161 (= R174), G222 (= G234), G223 (≠ Q235), G224 (= G236), T246 (= T260), L247 (≠ I261), M248 (= M262), L264 (= L278), G286 (= G300), R288 (= R302), D290 (= D304), V293 (= V307), D310 (= D324), I311 (= I325), D329 (= D343), V330 (≠ C344), Q404 (= Q417), M405 (= M418), G423 (= G436)
binding magnesium ion: D453 (= D466), N480 (= N493), H482 (≠ F495)
binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V400 (= V413), G401 (= G414), Q402 (= Q415), H403 (= H416), M428 (= M441), D453 (= D466), G454 (= G467), S455 (≠ G468), N480 (= N493), H482 (≠ F495), L483 (= L496), G484 (= G497), M485 (= M498), V486 (= V499)

8et4A Crystal structure of wild-type arabidopsis thaliana acetohydroxyacid synthase in complex with amidosulfuron (see paper)
42% identity, 95% coverage: 26:580/583 of query aligns to 14:575/582 of 8et4A

query
sites
8et4A

G

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K

A

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binding 2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-2-[(1~{S})-1-(dioxidanyl)-1-oxidanyl-ethyl]-4-methyl-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: V400 (= V413), G401 (= G414), Q402 (= Q415), H403 (= H416), G426 (= G439), M428 (= M441), G452 (= G465), D453 (= D466), G454 (= G467), S455 (≠ G468), M458 (= M471), N480 (= N493), H482 (≠ F495), L483 (= L496), G484 (= G497), M485 (= M498), V486 (= V499)
binding flavin-adenine dinucleotide: R161 (= R174), G222 (= G234), G223 (≠ Q235), G224 (= G236), T246 (= T260), L247 (≠ I261), M248 (= M262), L264 (= L278), M266 (= M280), H267 (= H281), G286 (= G300), V287 (≠ M301), R288 (= R302), D290 (= D304), R292 (= R306), V293 (= V307), D310 (= D324), I311 (= I325), D329 (= D343), V330 (≠ C344), M405 (= M418), G423 (= G436)
binding magnesium ion: F370 (≠ Y384), D453 (= D466), M458 (= M471), Q461 (= Q474), N480 (= N493), H482 (≠ F495), K533 (≠ R538)
binding N-{[(4,6-dimethoxypyrimidin-2-yl)carbamoyl]sulfamoyl}-N-methylmethanesulfonamide: M266 (= M280), R292 (= R306), M485 (= M498), W489 (= W502), S568 (≠ Q573)

5wj1A Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a triazolopyrimidine herbicide, penoxsulam (see paper)
42% identity, 95% coverage: 26:580/583 of query aligns to 14:575/582 of 5wj1A

query
sites
5wj1A

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active site: Y33 (= Y45), G35 (= G47), G36 (= G48), A37 (= A49), S38 (≠ I50), E59 (= E72), T82 (= T95), F121 (= F134), Q122 (= Q135), E123 (= E136), K171 (= K184), M266 (= M280), V293 (= V307), V400 (= V413), G426 (= G439), M428 (= M441), D453 (= D466), N480 (= N493), H482 (≠ F495), L483 (= L496), M485 (= M498), V486 (= V499), W489 (= W502), H558 (≠ R563)
binding flavin-adenine dinucleotide: R161 (= R174), G222 (= G234), G223 (≠ Q235), G224 (= G236), T246 (= T260), L247 (≠ I261), M248 (= M262), M263 (= M277), L264 (= L278), G286 (= G300), R288 (= R302), V293 (= V307), D310 (= D324), I311 (= I325), D329 (= D343), V330 (≠ C344), M405 (= M418), G423 (= G436), G424 (= G437)
binding magnesium ion: D453 (= D466), N480 (= N493), H482 (≠ F495)
binding 2-(2,2-difluoroethoxy)-N-(5,8-dimethoxy[1,2,4]triazolo[1,5-c]pyrimidin-2-yl)-6-(trifluoromethyl)benzenesulfonamide: M266 (= M280), D291 (= D305), R292 (= R306), M485 (= M498), W489 (= W502), S568 (≠ Q573)
binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V400 (= V413), G401 (= G414), Q402 (= Q415), H403 (= H416), M428 (= M441), D453 (= D466), G454 (= G467), S455 (≠ G468), M458 (= M471), N480 (= N493), H482 (≠ F495), L483 (= L496), G484 (= G497), M485 (= M498), V486 (= V499)

5k6tA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylamino-carbonyl-triazolinone herbicide, propoxycarbazone-sodium (see paper)
42% identity, 95% coverage: 26:580/583 of query aligns to 14:575/582 of 5k6tA

query
sites
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