SitesBLAST
Comparing CA265_RS15860 CA265_RS15860 threonine dehydratase to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
1tdjA Threonine deaminase (biosynthetic) from e. Coli (see paper)
35% identity, 93% coverage: 25:409/416 of query aligns to 28:409/494 of 1tdjA
- active site: K58 (= K55), A83 (= A80), E209 (= E209), S213 (≠ A213), C215 (≠ S215), G237 (= G237), L310 (≠ V308), S311 (= S309)
- binding pyridoxal-5'-phosphate: F57 (≠ Y54), K58 (= K55), N85 (= N82), G184 (= G184), G185 (= G185), G186 (= G186), G187 (= G187), G237 (= G237), E282 (= E282), S311 (= S309), G312 (= G310)
P04968 L-threonine dehydratase biosynthetic IlvA; Threonine deaminase; EC 4.3.1.19 from Escherichia coli (strain K12) (see paper)
35% identity, 93% coverage: 25:409/416 of query aligns to 32:413/514 of P04968
- K62 (= K55) modified: N6-(pyridoxal phosphate)lysine
- N89 (= N82) binding
- GGGGL 188:192 (= GGGGL 184:188) binding
- S315 (= S309) binding
2gn2A Crystal structure of tetrameric biodegradative threonine deaminase (tdcb) from salmonella typhimurium in complex with cmp at 2.5a resolution (hexagonal form) (see paper)
38% identity, 75% coverage: 10:321/416 of query aligns to 11:321/326 of 2gn2A
- active site: K56 (= K55), A81 (= A80), Q207 (≠ E209), V211 (≠ A213), G213 (≠ S215), G235 (= G237), I308 (≠ V308), S309 (= S309)
- binding cytidine-5'-monophosphate: R51 (≠ I50), T52 (≠ V51), G53 (≠ R52), A114 (≠ Q113), D117 (≠ M116), Y118 (≠ F117), N312 (= N312)
O59791 Serine racemase; D-serine ammonia-lyase; D-serine dehydratase; L-serine ammonia-lyase; L-serine dehydratase; EC 4.3.1.17; EC 4.3.1.18; EC 5.1.1.18 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
34% identity, 76% coverage: 2:317/416 of query aligns to 4:316/323 of O59791
- S82 (≠ A80) mutation to A: Loss of racemase activity. Reduces D-serine dehydratase activity by 99%. Slightly reduced L-serine dehydratase activity.
2zr8A Crystal structure of modified serine racemase complexed with serine (see paper)
34% identity, 75% coverage: 6:317/416 of query aligns to 4:312/319 of 2zr8A
- active site: K53 (= K55), S78 (≠ A80), E204 (= E209), G208 (≠ A213), D210 (≠ S215), G232 (= G237), I303 (≠ V308), S304 (= S309)
- binding magnesium ion: E204 (= E209), G208 (≠ A213), D210 (≠ S215)
- binding n-(5'-phosphopyridoxyl)-d-alanine: F52 (≠ Y54), K53 (= K55), S77 (= S79), S78 (≠ A80), N80 (= N82), H81 (= H83), P147 (= P152), G179 (= G184), G180 (= G185), G181 (= G186), G182 (= G187), G232 (= G237), E277 (= E282), T279 (≠ A284), S304 (= S309)
- binding serine: S78 (≠ A80), R129 (≠ C134), D231 (= D236), G232 (= G237), A233 (= A238), Q234 (≠ A239), T235 (≠ V240)
2zpuA Crystal structure of modified serine racemase from s.Pombe. (see paper)
34% identity, 75% coverage: 6:317/416 of query aligns to 4:312/319 of 2zpuA
- active site: K53 (= K55), S78 (≠ A80), E204 (= E209), G208 (≠ A213), D210 (≠ S215), G232 (= G237), I303 (≠ V308), S304 (= S309)
- binding magnesium ion: E204 (= E209), G208 (≠ A213), D210 (≠ S215)
- binding n-(5'-phosphopyridoxyl)-d-alanine: F52 (≠ Y54), K53 (= K55), S77 (= S79), S78 (≠ A80), N80 (= N82), H81 (= H83), P147 (= P152), G179 (= G184), G180 (= G185), G181 (= G186), G182 (= G187), G232 (= G237), E277 (= E282), T279 (≠ A284), S304 (= S309)
1wtcA Crystal structure of s.Pombe serine racemase complex with amppcp (see paper)
34% identity, 75% coverage: 6:317/416 of query aligns to 3:311/318 of 1wtcA
- active site: K52 (= K55), S77 (≠ A80), E203 (= E209), G207 (≠ A213), D209 (≠ S215), G231 (= G237), I302 (≠ V308), S303 (= S309)
- binding phosphomethylphosphonic acid adenylate ester: N20 (≠ K23), K47 (≠ I50), M48 (≠ V51), A109 (≠ K112), A110 (≠ Q113), Y114 (≠ F117)
- binding magnesium ion: E203 (= E209), G207 (≠ A213), D209 (≠ S215)
- binding pyridoxal-5'-phosphate: F51 (≠ Y54), K52 (= K55), N79 (= N82), G178 (= G184), G179 (= G185), G180 (= G186), G181 (= G187), G231 (= G237), E276 (= E282), T278 (≠ A284), S303 (= S309)
1v71A Crystal structure of s.Pombe serine racemase
34% identity, 75% coverage: 6:317/416 of query aligns to 3:311/318 of 1v71A
- active site: K52 (= K55), S77 (≠ A80), E203 (= E209), G207 (≠ A213), D209 (≠ S215), G231 (= G237), I302 (≠ V308), S303 (= S309)
- binding magnesium ion: E203 (= E209), G207 (≠ A213), D209 (≠ S215)
- binding pyridoxal-5'-phosphate: F51 (≠ Y54), K52 (= K55), N79 (= N82), G178 (= G184), G179 (= G185), G180 (= G186), G181 (= G187), G231 (= G237), E276 (= E282), T278 (≠ A284), S303 (= S309), G304 (= G310)
A4F2N8 L-threo-3-hydroxyaspartate ammonia-lyase; L-threo-3-hydroxyaspartate dehydratase; L-THA DH; EC 4.3.1.16 from Pseudomonas sp. (see paper)
32% identity, 74% coverage: 10:317/416 of query aligns to 8:312/319 of A4F2N8
- K53 (= K55) mutation to A: Loss of enzymatic activity.
Q7XSN8 Serine racemase; D-serine dehydratase; D-serine ammonia-lyase; L-serine dehydratase; L-serine ammonia-lyase; EC 5.1.1.18; EC 4.3.1.18; EC 4.3.1.17 from Oryza sativa subsp. japonica (Rice) (see paper)
30% identity, 75% coverage: 11:320/416 of query aligns to 24:334/339 of Q7XSN8
- E219 (= E209) mutation to A: Reduces catalytic activity and abolishes the regulatory effect of Mg(2+) addition; when associated with A-225.
- D225 (≠ S215) mutation to A: Reduces catalytic activity and abolishes the regulatory effect of Mg(2+) addition; when associated with A-219.
5cvcA Structure of maize serine racemase (see paper)
29% identity, 78% coverage: 10:334/416 of query aligns to 7:327/329 of 5cvcA
- active site: K52 (= K55), S77 (≠ A80), E203 (= E209), A207 (= A213), D209 (≠ S215), G231 (= G237), V306 (= V308), S307 (= S309)
- binding magnesium ion: E203 (= E209), A207 (= A213), D209 (≠ S215)
- binding pyridoxal-5'-phosphate: F51 (≠ Y54), K52 (= K55), N79 (= N82), S178 (≠ G184), G179 (= G185), G180 (= G186), G181 (= G187), L232 (≠ A238), E275 (= E282), S307 (= S309), G308 (= G310)
7nbfAAA structure of human serine racemase in complex with DSiP fragment Z126932614, XChem fragment screen (see paper)
30% identity, 74% coverage: 10:315/416 of query aligns to 8:316/323 of 7nbfAAA
- active site: K53 (= K55), S81 (≠ A80), E207 (= E209), A211 (= A213), D213 (≠ S215), G236 (= G237), L309 (vs. gap), S310 (= S309)
- binding calcium ion: E207 (= E209), A211 (= A213), D213 (≠ S215)
- binding magnesium ion: N244 (≠ I246)
- binding pyridoxal-5'-phosphate: F52 (≠ Y54), K53 (= K55), N83 (= N82), G182 (= G184), G183 (= G185), G184 (= G186), G185 (= G187), M186 (≠ L188), G236 (= G237), V237 (≠ A238), T282 (≠ A284), S310 (= S309), G311 (= G310)
- binding 2-[(methylsulfonyl)methyl]-1H-benzimidazole: H21 (≠ K23), L22 (≠ R24), T23 (= T25), P24 (= P26), L26 (≠ E28), T27 (≠ F29), F46 (≠ L48)
Sites not aligning to the query:
7nbdAAA structure of human serine racemase in complex with DSiP fragment Z235449082, XChem fragment screen (see paper)
30% identity, 74% coverage: 10:315/416 of query aligns to 8:316/323 of 7nbdAAA
- active site: K53 (= K55), S81 (≠ A80), E207 (= E209), A211 (= A213), D213 (≠ S215), G236 (= G237), L309 (vs. gap), S310 (= S309)
- binding calcium ion: E207 (= E209), A211 (= A213), D213 (≠ S215)
- binding [4-(1H-benzimidazol-1-yl)phenyl]methanol: W272 (≠ Y274), L278 (≠ V280), V314 (≠ N313), L316 (≠ I315)
- binding magnesium ion: N244 (≠ I246)
- binding pyridoxal-5'-phosphate: F52 (≠ Y54), K53 (= K55), N83 (= N82), G182 (= G184), G183 (= G185), G184 (= G186), G185 (= G187), M186 (≠ L188), G236 (= G237), V237 (≠ A238), E280 (= E282), T282 (≠ A284), S310 (= S309), G311 (= G310)
Sites not aligning to the query:
7nbcCCC structure of human serine racemase in complex with DSiP fragment Z2856434779, XChem fragment screen (see paper)
30% identity, 74% coverage: 10:315/416 of query aligns to 8:316/323 of 7nbcCCC
- active site: K53 (= K55), S81 (≠ A80), E207 (= E209), A211 (= A213), D213 (≠ S215), G236 (= G237), L309 (vs. gap), S310 (= S309)
- binding biphenyl-4-ylacetic acid: T78 (≠ C77), H79 (≠ A78), H84 (= H83), V148 (≠ I150), H149 (≠ P151), P150 (= P152)
- binding calcium ion: E207 (= E209), A211 (= A213), D213 (≠ S215)
- binding pyridoxal-5'-phosphate: F52 (≠ Y54), K53 (= K55), N83 (= N82), G182 (= G184), G183 (= G185), G184 (= G186), G185 (= G187), M186 (≠ L188), G236 (= G237), V237 (≠ A238), T282 (≠ A284), S310 (= S309), G311 (= G310)
7nbcAAA structure of human serine racemase in complex with DSiP fragment Z2856434779, XChem fragment screen (see paper)
30% identity, 74% coverage: 10:315/416 of query aligns to 8:316/323 of 7nbcAAA
- active site: K53 (= K55), S81 (≠ A80), E207 (= E209), A211 (= A213), D213 (≠ S215), G236 (= G237), L309 (vs. gap), S310 (= S309)
- binding calcium ion: E207 (= E209), A211 (= A213), D213 (≠ S215)
- binding magnesium ion: N244 (≠ I246)
- binding pyridoxal-5'-phosphate: F52 (≠ Y54), K53 (= K55), N83 (= N82), G182 (= G184), G183 (= G185), G184 (= G186), G185 (= G187), M186 (≠ L188), G236 (= G237), V237 (≠ A238), T282 (≠ A284), S310 (= S309), G311 (= G310)
Sites not aligning to the query:
7nbgAAA structure of human serine racemase in complex with DSiP fragment Z52314092, XChem fragment screen (see paper)
30% identity, 74% coverage: 10:315/416 of query aligns to 8:316/322 of 7nbgAAA
- active site: K53 (= K55), S81 (≠ A80), E207 (= E209), A211 (= A213), D213 (≠ S215), G236 (= G237), L309 (vs. gap), S310 (= S309)
- binding calcium ion: E207 (= E209), A211 (= A213), D213 (≠ S215)
- binding pyridoxal-5'-phosphate: F52 (≠ Y54), K53 (= K55), N83 (= N82), G182 (= G184), G183 (= G185), G184 (= G186), G185 (= G187), M186 (≠ L188), G236 (= G237), V237 (≠ A238), T282 (≠ A284), S310 (= S309), G311 (= G310)
- binding ~{N}-[2-(2-methylphenyl)ethyl]ethanamide: S81 (≠ A80), G85 (≠ A84), Q86 (= Q85), I101 (= I100), K111 (= K110), I115 (≠ T114), Y118 (≠ F117)
7nbhAAA structure of human serine racemase in complex with DSiP fragment Z26781964, XChem fragment screen (see paper)
30% identity, 74% coverage: 10:315/416 of query aligns to 8:316/320 of 7nbhAAA
- active site: K53 (= K55), S81 (≠ A80), E207 (= E209), A211 (= A213), D213 (≠ S215), G236 (= G237), L309 (vs. gap), S310 (= S309)
- binding calcium ion: E207 (= E209), A211 (= A213), D213 (≠ S215)
- binding N-[(1H-benzimidazol-2-yl)methyl]furan-2-carboxamide: S81 (≠ A80), G85 (≠ A84), Q86 (= Q85), K111 (= K110), I115 (≠ T114), Y118 (≠ F117), D235 (= D236), P281 (= P283), N313 (= N312), V314 (≠ N313), D315 (= D314)
1ve5A Crystal structure of t.Th. Hb8 threonine deaminase
33% identity, 74% coverage: 6:314/416 of query aligns to 1:305/308 of 1ve5A
- active site: K50 (= K55), S56 (≠ N61), S72 (≠ A80), E200 (= E209), A204 (= A213), D206 (≠ S215), G229 (= G237), L299 (≠ V308), S300 (= S309)
- binding calcium ion: E200 (= E209), A204 (= A213), D206 (≠ S215)
- binding pyridoxal-5'-phosphate: F49 (≠ Y54), K50 (= K55), N74 (= N82), G175 (= G184), G176 (= G185), G177 (= G186), G178 (= G187), E274 (= E282), T276 (≠ A284), S300 (= S309), G301 (= G310)
6zspAAA serine racemase bound to atp and malonate. (see paper)
29% identity, 74% coverage: 10:315/416 of query aligns to 8:309/320 of 6zspAAA
- active site: K53 (= K55), S74 (≠ A80), E200 (= E209), A204 (= A213), D206 (≠ S215), G229 (= G237), L302 (vs. gap), S303 (= S309)
- binding adenosine-5'-triphosphate: S28 (≠ N30), S29 (≠ A31), I30 (≠ G32), K48 (≠ I50), T49 (≠ V51), Q79 (= Q85), Y111 (≠ F117), E266 (≠ N275), R267 (≠ E276), K269 (≠ A278), N306 (= N312)
- binding magnesium ion: E200 (= E209), A204 (= A213), D206 (≠ S215)
- binding malonate ion: K53 (= K55), S73 (= S79), S74 (≠ A80), N76 (= N82), H77 (= H83), R125 (≠ F131), G229 (= G237), S232 (≠ K241)
3l6bA X-ray crystal structure of human serine racemase in complex with malonate a potent inhibitor (see paper)
29% identity, 74% coverage: 10:315/416 of query aligns to 9:312/322 of 3l6bA
- active site: K54 (= K55), S77 (≠ A80), E203 (= E209), A207 (= A213), D209 (≠ S215), G232 (= G237), T278 (≠ A284), L305 (vs. gap), S306 (= S309)
- binding malonate ion: K54 (= K55), S76 (= S79), S77 (≠ A80), N79 (= N82), H80 (= H83), R128 (≠ F131), G232 (= G237)
- binding manganese (ii) ion: E203 (= E209), A207 (= A213), D209 (≠ S215)
- binding pyridoxal-5'-phosphate: F53 (≠ Y54), K54 (= K55), N79 (= N82), G178 (= G184), G179 (= G185), G180 (= G186), G181 (= G187), M182 (≠ L188), V233 (≠ A238), E276 (= E282), T278 (≠ A284), S306 (= S309), G307 (= G310)
Query Sequence
>CA265_RS15860 CA265_RS15860 threonine dehydratase
MNTTTPNTLDFQSASQRLKGVVKRTPLEFNAGLSAHYNANIYLKREDLQIVRSYKLRGAY
NKISSLPQDALINGVVCASAGNHAQGVAYSCKKLGIKGVIFMPEITPKQKVKQTYMFGGD
NVEVVLVGDTFDDCLKEALAYSAEKSATFIPPFDDEKVIEGQATVGVEIYEDLPDLDMIV
MPVGGGGLASGVSAYMKTVKPEVKLVGVEPLGAPSMVTAMEYGGPFTLEEIDRFVDGAAV
KRIGHITYEYCKELLDQMHLIPEGKICTTILKLYNEDAIVVEPAGALSVAALDQLKDQIT
GKTVVCIVSGGNNDIERMQEIKEKSLLFEGLKHYFIVRFPQRPGALKLFVNEVLGPQDDI
TRFEFIKKTNKENGPALVGIELSNKNDYASLLQRMKDFKFEIIELNQDQTLFEYLV
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory