SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing CA265_RS18400 FitnessBrowser__Pedo557:CA265_RS18400 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 1 hits to proteins with known functional sites (download)

7bkbE Formate dehydrogenase - heterodisulfide reductase - formylmethanofuran dehydrogenase complex from methanospirillum hungatei (hexameric, composite structure) (see paper)
37% identity, 14% coverage: 310:374/461 of query aligns to 292:355/411 of 7bkbE

query
sites
7bkbE

C
|
C

I
|
I

R
x
K

C
|
C

G
x
Y

A
|
A

C
|
C

L

V

N

E

A

A

C
|
C

P
|
P

V

I

Y
x
C

K

Y

N
x
C

I

I

G

E

G
x
C

H
x
S

T

T

Y

K

N

K

T

P

T

W

Y

Y

S

I

G

A

P

P

I

-

G

G

S

V

V

L

I

P

T

T

P

S

H

F

L

M

K
x
F

G

H

M

L

E

I

E

R

F

F

K

A

H
|
H

L

V

S

S

Y

D

A

S

S
x
C

S
x
I

L
x
N

C
|
C

G
|
G

K

Q

C
|
C

S

E

E

E

V

L

C
|
C

P
|
P

V

M

K

E

I

I

binding iron/sulfur cluster: C292 (= C310), I293 (= I311), K294 (≠ R312), C295 (= C313), Y296 (≠ G314), A297 (= A315), C298 (= C316), C302 (= C320), P303 (= P321), C305 (≠ Y323), C307 (≠ N325), C310 (≠ G328), S311 (≠ H329), F329 (≠ K348), H336 (= H355), C341 (≠ S360), I342 (≠ S361), N343 (≠ L362), C344 (= C363), G345 (= G364), C347 (= C366), C351 (= C370), P352 (= P371)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 20, 21, 22, 24, 26, 27, 45, 68, 69, 70, 71, 72, 73, 75, 98, 127, 128, 129, 205, 206, 207, 218
binding iron/sulfur cluster: 101, 102, 128, 189, 192, 357, 361

Query Sequence

>CA265_RS18400 FitnessBrowser__Pedo557:CA265_RS18400
MNIAEEFLEKSDEKAFDLPHRKTINYNIGKYNAAVERGLSKFENLEASKKKAHVIKWRVM
ENLDKFLPEFESNFQRRGGKVIWANDAEEAQQEILNIIKRNNGKTVIKSKSMTTEEIHLN
DFLEKNNIESLESDLGEYIVQLLGQAPYHIVTPAMHLSATDIAQLFHDKFGTPIDATPPQ
LVQKARELLRDKYLNADIGISGGNFLIADTGSIALTENEGNARLSTTFPKIHIAIVGIEK
VIPSIADLDLFWPLLASHGTGQNLTVYNTILSGPRQPNETDGPEEMYVILLDNGRTNLLA
QKDQRQGLYCIRCGACLNACPVYKNIGGHTYNTTYSGPIGSVITPHLKGMEEFKHLSYAS
SLCGKCSEVCPVKIDIHKMLLLNRRDAAAGHENGKKEEIGWSMFSRMMQKRKWMDFFGGK
FKNFMLKRFFKKSWGKYREMPKVADKSFAKQWEEMKKSKES

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory