Comparing CA265_RS18440 CA265_RS18440 glycoside hydrolase to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
Q96TU3 Extracellular exo-inulinase inuE; EC 3.2.1.80 from Aspergillus awamori (Black koji mold) (see 2 papers)
36% identity, 95% coverage: 8:490/506 of query aligns to 8:520/537 of Q96TU3
Sites not aligning to the query:
1y9gA Crystal structure of exo-inulinase from aspergillus awamori complexed with fructose (see paper)
37% identity, 92% coverage: 27:490/506 of query aligns to 7:501/517 of 1y9gA
O94220 Extracellular endo-inulinase inu2; 2,1-beta-D-fructanfructanohydrolase; Inulase; EC 3.2.1.7 from Aspergillus ficuum (see 2 papers)
31% identity, 98% coverage: 8:503/506 of query aligns to 9:514/516 of O94220
3rwkX First crystal structure of an endo-inulinase, from aspergillus ficuum: structural analysis and comparison with other gh32 enzymes. (see paper)
32% identity, 94% coverage: 27:503/506 of query aligns to 5:491/493 of 3rwkX
6s2bA Structure of beta-fructofuranosidase from schwanniomyces occidentalis complexed with fructosyl-erythritol (see paper)
35% identity, 78% coverage: 28:421/506 of query aligns to 13:418/512 of 6s2bA
6s1tA Structure of beta-fructofuranosidase from schwanniomyces occidentalis complexed with sucrose (see paper)
35% identity, 78% coverage: 28:421/506 of query aligns to 13:418/512 of 6s1tA
P00724 Invertase 2; Beta-fructofuranosidase 2; Saccharase; EC 3.2.1.26 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 2 papers)
31% identity, 89% coverage: 28:475/506 of query aligns to 28:501/532 of P00724
Sites not aligning to the query:
8betA Structure of d188a-fructofuranosidase from rhodotorula dairenesis in complex with sucrose (see paper)
30% identity, 92% coverage: 24:489/506 of query aligns to 29:507/525 of 8betA
Sites not aligning to the query:
8beqA Structure of fructofuranosidase from rhodotorula dairenensis (see paper)
29% identity, 92% coverage: 24:489/506 of query aligns to 31:515/534 of 8beqA
Sites not aligning to the query:
8besA Structure of d188a-fructofuranosidase from rhodotorula dairenensis in complex with fructose (see paper)
30% identity, 92% coverage: 24:489/506 of query aligns to 29:508/526 of 8besA
Sites not aligning to the query:
7bwcA Bombyx mori gh32 beta-fructofuranosidase bmsuc1 mutant d63a in complex with sucrose (see paper)
31% identity, 90% coverage: 25:477/506 of query aligns to 22:438/464 of 7bwcA
4ffgA Crystal structure of levan fructotransferase from arthrobacter ureafaciens in complex with dfa-iv (see paper)
28% identity, 93% coverage: 31:499/506 of query aligns to 3:474/480 of 4ffgA
4ffhA Crystal structure of levan fructotransferase d54n mutant from arthrobacter ureafaciens in complex with sucrose (see paper)
28% identity, 93% coverage: 31:499/506 of query aligns to 3:474/480 of 4ffhA
7vcpA Frischella perrara beta-fructofuranosidase in complex with fructose (see paper)
28% identity, 97% coverage: 15:506/506 of query aligns to 13:489/490 of 7vcpA
O33833 Beta-fructosidase; Invertase; Sucrase; EC 3.2.1.26 from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) (see paper)
29% identity, 94% coverage: 27:500/506 of query aligns to 2:431/432 of O33833
1w2tB Beta-fructosidase from thermotoga maritima in complex with raffinose (see paper)
28% identity, 94% coverage: 27:500/506 of query aligns to 2:431/432 of 1w2tB
1w2tA Beta-fructosidase from thermotoga maritima in complex with raffinose (see paper)
28% identity, 94% coverage: 27:500/506 of query aligns to 2:431/432 of 1w2tA
3pijB Beta-fructofuranosidase from bifidobacterium longum - complex with fructose (see paper)
27% identity, 97% coverage: 14:502/506 of query aligns to 26:512/526 of 3pijB
6nunA Structure of gh32 hydrolase from bifidobacterium adolescentis in complex with frutose
26% identity, 94% coverage: 26:502/506 of query aligns to 36:510/516 of 6nunA
3uggA Crystal structure of a 6-sst/6-sft from pachysandra terminalis in complex with 1-kestose (see paper)
25% identity, 94% coverage: 28:503/506 of query aligns to 13:516/524 of 3uggA
>CA265_RS18440 CA265_RS18440 glycoside hydrolase
MFKTKYILAFLMCCSISTFAQQPTPQWRPTYHFTAPSNWLNDPNGLIYLNGEFHLYYQYN
PFENKWGHMSWGHATSKDLVNWKHLPVAIPEKITKDTTTMIFSGSAVLDKRNVSGLGKGK
APVVAFYTADLPKQRNESQYMAYSNDGGLSFTNYAKNPIIDLNKKDFRDPNVWWHESTQQ
WIMTVAMVDEHQARFYGSKNLKDWTMLSDFGKQGNQGSGWECPFIIPLAVDGNKDNTKWV
LAISLFGPKGPTMQYFVGDFDGKTFKNDNDGKLTLLVDEGDSFYAAIPWNDAPKRQRILL
GWLQPGGKETFPWKGQMSIPRDLALKTTPEGIRLFQQPASLIGDNMKKHSGGKVITRQNL
IIDQHIDLGTSQNSYWIDAEFSVSDAEKIGFKLAQLKDEKGNVVKEVEVGYDAIKKQLYV
DCTRSEKGIKNDKNIIQTAAVNSVNGKIKLQVLFDKSSLEVFGNAGEKVISTMIFPDEKA
TGLAAFSNGGKAVLNKLKVWDMSGRK
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SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.
Lawrence Berkeley National Laboratory