SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing CA265_RS18510 FitnessBrowser__Pedo557:CA265_RS18510 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q9HTN2 Acetylglutamate kinase; N-acetyl-L-glutamate 5-phosphotransferase; NAG kinase; NAGK; EC 2.7.2.8 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see 2 papers)
34% identity, 90% coverage: 8:258/278 of query aligns to 31:272/301 of Q9HTN2

query
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Q9HTN2

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R90 (= R64) binding substrate
N195 (= N175) binding substrate

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

2bufC Arginine feed-back inhibitable acetylglutamate kinase (see paper)
34% identity, 90% coverage: 8:258/278 of query aligns to 30:271/298 of 2bufC

query
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2bufC

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active site: K32 (= K10), G35 (= G13), G68 (= G43), D198 (= D179), K254 (= K241)
binding adenosine-5'-diphosphate: N36 (= N14), N218 (= N209), L222 (vs. gap), M223 (vs. gap), T246 (= T233), I247 (≠ V234), Y248 (≠ Q235), G250 (= G237), M251 (= M238)

2bufA Arginine feed-back inhibitable acetylglutamate kinase (see paper)
34% identity, 90% coverage: 8:258/278 of query aligns to 30:263/292 of 2bufA

query
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2bufA

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active site: K32 (= K10), G35 (= G13), G68 (= G43), D190 (= D179), K246 (= K241)
binding n-acetyl-l-glutamate: G66 (= G41), G67 (= G42), R89 (= R64), N186 (= N175), A189 (= A178)

2v5hB Controlling the storage of nitrogen as arginine: the complex of pii and acetylglutamate kinase from synechococcus elongatus pcc 7942 (see paper)
32% identity, 91% coverage: 5:258/278 of query aligns to 23:258/289 of 2v5hB

query
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2v5hB

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active site: K28 (= K10), G31 (= G13), G64 (= G43), D182 (= D179), K241 (= K241)
binding n-acetyl-l-glutamate: G62 (= G41), R85 (= R64), N178 (= N175), A181 (= A178)

2btyA Acetylglutamate kinase from thermotoga maritima complexed with its inhibitor arginine (see paper)
36% identity, 90% coverage: 8:258/278 of query aligns to 25:254/282 of 2btyA

query
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2btyA

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C

L

F

T

E

D

K

V

K

D

G

G

V

V

L

L

K

K

N

-

V

-

N

-

D

-

D

D

G

G

S

K

V

L

V

I

R
x
S

E

T

I

L

K

T

A

P

D

D

E

E

F

A

E

E

G

E

L

L

K

I

A

R

D

D

G

G

T

T

V

V

Q

T

G

G

M

M

I

I

P

P

K
|
K

L

V

H

E

N

C

A

A

F

V

E

S

A

A

I

V

K

R

K

G

G

G

V

V

S

G

A

A

V

V

Y

H

I

I

active site: K27 (= K10), G30 (= G13), G63 (= G43), D182 (= D179), K237 (= K241)
binding arginine: K196 (≠ R196), S214 (≠ R218)
binding n-acetyl-l-glutamate: G29 (= G12), G30 (= G13), G61 (= G41), G62 (= G42), N180 (= N177), D182 (= D179)

Sites not aligning to the query:

binding arginine: 15, 19, 266, 267, 270, 271, 274, 276

Q9X2A4 Acetylglutamate kinase; N-acetyl-L-glutamate 5-phosphotransferase; NAG kinase; NAGK; EC 2.7.2.8 from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) (see paper)
36% identity, 90% coverage: 8:258/278 of query aligns to 25:254/282 of Q9X2A4

query
sites
Q9X2A4

I

V

I

I

K

K

I

F

G

G

N

S

V

A

I

M

D

K

N

Q

S

E

E

N

N

A

L

K

H

K

Q

A

F

F

L

I

L

Q

D

D

F

I

T

I

A

L

L

L

P

K

-

Y

-

T

G

G

D

I

K

K

-

P

I

I

L

I

V

V

H

H

G

G

K

P

I

A

A

I

T

S

E

Q

L

M

G

M

E

K

S

D

L

L

G

G

I

I

E

E

A

P

K

V

M

F

I

K

D

N

G

G

R

H

R

R

I

V

T

T

D

D

I

E

E

K

T

T

L

M

R

E

I

I

V

V

T

E

M

M

V

V

Y

L

A

V

G

G

L

K

I

I

N

N

K

K

N

E

M

I

V

V

A

M

Q

N

L

L

Q

N

A

L

K

H

G

G

S

G

N

R

A

A

I

V

G

G

L

I

S

C

G

G

A

K

D

D

G

S

N

K

V

L

I

I

R

V

A

A

K

E

K

K

R

E

P

-

V

-

I

-

T

T

K

K

T

-

Y

H

G

G

A

-

E

-

S

-

G

-

P

-

M

-

A

-

G

-

A

-

V

-

I

-

D

D

Y

I

G

G

F

Y

V

V

G

G

D

K

L

V

D

K

E

K

N

-

A

-

V

V

S

N

S

P

T

E

T

I

L

L

E

H

S

A

L

L

L

I

K

E

A

N

G

D

L

Y

V

I

P

P

V

V

L

I

C

A

A

P

I

V

T

G

H

I

D

G

G

E

D

D

T

G

Q

H

L

S

L

Y

N
|
N

T

I

N

N

A

A

D

D

T

T

I

A

A

A

S

A

S

E

V

I

A

A

V

-

A

-

M

-

S

K

A

S

L

L

Y

M

E

A

T

E

R
x
K

L

L

V

I

Y

L

C

L

F

T

E

D

K

V

K

D

G

G

V

V

L

L

K

K

N

-

V

-

N

-

D

-

D

D

G

G

S

K

V

L

V

I

R
x
S

E

T

I

L

K

T

A

P

D

D

E

E

F

A

E

E

G

E

L

L

K

I

A

R

D

D

G

G

T

T

V

V

Q

T

G

G

M

M

I

I

P

P

K

K

L

V

H

E

N

C

A

A

F

V

E

S

A

A

I

V

K

R

K

G

G

G

V

V

S

G

A

A

V

V

Y

H

I

I

N178 (= N175) binding substrate
K196 (≠ R196) binding L-arginine
S214 (≠ R218) binding L-arginine

Sites not aligning to the query:

266:269 binding L-arginine

Q9SCL7 Acetylglutamate kinase, chloroplastic; N-acetyl-L-glutamate 5-phosphotransferase; NAG kinase; AtNAGK; EC 2.7.2.8 from Arabidopsis thaliana (Mouse-ear cress) (see 3 papers)
33% identity, 90% coverage: 8:258/278 of query aligns to 89:322/347 of Q9SCL7

query
sites
Q9SCL7

I

V

K

K

I

Y

G

G

G
|
G

N
x
A

V

A

I

M

D

T

N

S

S

P

E

E

N

L

L

K

H

S

Q

S

F

V

L

V

L

S

D

D

F

L

T

V

A

L

L

L

P

A

G

C

-

V

-

G

-

L

D

R

K

P

I

I

L

L

V

V

H

H

G

G

G
|
G

G

G

K

P

I

D

A

I

T

N

E

R

L

Y

G

L

E

K

S

Q

L

L

G

N

I

I

E

P

A

A

K

E

M

F

I

R

D

D

G

G

R

L

R
|
R

I

V

T

T

D

D

I

A

E

T

T

T

L

M

R

E

I

I

V

V

T

S

M

M

V

V

Y

L

A

V

G

G

L

K

I

V

N

N

K

K

N

N

M

L

V

V

A

S

Q

L

L

I

Q

N

A

A

K

A

G

G

S

A

N

T

A

A

I

V

G

G

L

L

S

S

G

G

A

H

D

D

G

G

N

R

V

L

I

L

R

T

A

A

K

-

R

R

P

P

V

V

I

P

T

N

K

S

T

A

Y

-

G

-

A

-

E

-

S

-

G

-

P

-

M

-

A

-

G

-

A

-

V

-

I

-

D

Q

Y

L

G

G

F

F

V

V

G

G

D

E

L

V

D

A

E

R

N

-

A

-

V

V

S

D

S

P

T

S

T

V

L

L

E

R

S

P

L

L

L

V

K

D

A

Y

G

G

L

Y

V

I

P

P

V

V

L

I

C

A

A

S

I

V

T

A

H

A

D

D

G

D

D

S

T

G

Q

Q

L

A

L

Y

N
|
N

T
x
I

N
|
N

A
|
A

D

D

T

T

I

V

A

A

S

G

S

E

V

L

A

A

V

A

A

A

M

L

S

G

A

A

L

-

Y

-

E

-

T

E

R
x
K

L

L

V

I

Y

L

C

L

F
x
T

E
x
D

K

V

K

A

G

G

V

I

L
|
L

K

E

N

N

V

K

N

E

D

D

D

P

G

S

S

S

V

L

V

I

R
x
K

E

E

I

I

K

D

A

I

D

K

E

G

F

V

E

K

G

K

L

M

K

I

A

E

D

D

G

G

T
x
K

V
|
V

Q
x
A

G
|
G

M
|
M

I
|
I

P
|
P

K
|
K

L

V

H

K

N

C

A

C

F

I

E

R

A

S

I

L

K

A

K

Q

G

G

V

V

S

K

A

T

V

A

Y

S

I

I

GA 94:95 (≠ GN 13:14) binding ATP
G126 (= G42) binding N-acetyl-L-glutamate
R148 (= R64) binding N-acetyl-L-glutamate
NINA 242:245 (≠ NTNA 175:178) binding N-acetyl-L-glutamate
K260 (≠ R196) binding L-arginine
TD 265:266 (≠ FE 201:202) binding ATP
L271 (= L207) binding ATP
K282 (≠ R218) binding L-arginine
297:305 (vs. 233:241, 78% identical) binding ATP

Sites not aligning to the query:

1:50 modified: transit peptide, Chloroplast
334:337 binding L-arginine
342 binding L-arginine

2rd5B Structural basis for the regulation of n-acetylglutamate kinase by pii in arabidopsis thaliana (see paper)
33% identity, 90% coverage: 8:258/278 of query aligns to 25:258/283 of 2rd5B

query
sites
2rd5B

I

V

K
|
K

I

Y

G

G

G
|
G

N
x
A

V

A

I

M

D

T

N

S

S

P

E

E

N

L

L

K

H

S

Q

S

F

V

L

V

L

S

D

D

F

L

T

V

A

L

L

L

P

A

G

C

-

V

-

G

-

L

D

R

K

P

I

I

L

L

V

V

H

H

G
|
G

G

G

G
|
G

K

P

I

D

A

I

T

N

E

R

L

Y

G

L

E

K

S

Q

L

L

G

N

I

I

E

P

A

A

K

E

M

F

I

R

D

D

G

G

R

L

R
|
R

I

V

T

T

D

D

I

A

E

T

T

T

L

M

R

E

I

I

V

V

T

S

M

M

V

V

Y

L

A

V

G

G

L

K

I

V

N

N

K

K

N

N

M

L

V

V

A

S

Q

L

L

I

Q

N

A

A

K

A

G

G

S

A

N

T

A

A

I

V

G

G

L

L

S

S

G

G

A

H

D

D

G

G

N

R

V

L

I

L

R

T

A

A

K

-

R

R

P

P

V

V

I

P

T

N

K

S

T

A

Y

-

G

-

A

-

E

-

S

-

G

-

P

-

M

-

A

-

G

-

A

-

V

-

I

-

D

Q

Y

L

G

G

F

F

V

V

G

G

D

E

L

V

D

A

E

R

N

-

A

-

V

V

S

D

S

P

T

S

T

V

L

L

E

R

S

P

L

L

L

V

K

D

A

Y

G

G

L

Y

V

I

P

P

V

V

L

I

C

A

A

S

I

V

T

A

H

A

D

D

G

D

D

S

T

G

Q

Q

L

A

L

Y

N
|
N

T

I

N
|
N

A
|
A

D
|
D

T

T

I

V

A

A

S

G

S

E

V

L

A

A

V

A

A

A

M

L

S

G

A

A

L

-

Y

-

E

-

T

E

R
x
K

L

L

V

I

Y

L

C

L

F
x
T

E
x
D

K

V

K

A

G

G

V

I

L
|
L

K

E

N

N

V
x
K

N

E

D

D

D

P

G

S

S

S

V

L

V

I

R
x
K

E

E

I

I

K

D

A

I

D

K

E

G

F

V

E

K

G

K

L

M

K

I

A

E

D

D

G

G

T

K

V

V

Q
x
A

G

G

G
|
G

M
|
M

I

I

P

P

K
|
K

L

V

H

K

N

C

A

C

F

I

E

R

A

S

I

L

K

A

K

Q

G

G

V

V

S

K

A

T

V

A

Y

S

I

I

active site: K27 (= K10), G30 (= G13), G63 (= G43), D182 (= D179), K241 (= K241)
binding adenosine-5'-diphosphate: A31 (≠ N14), T201 (≠ F201), D202 (≠ E202), L207 (= L207), K210 (≠ V210), A235 (≠ Q235), G237 (= G237), M238 (= M238), K241 (= K241)
binding arginine: K196 (≠ R196), K218 (≠ R218)
binding n-acetyl-l-glutamate: G61 (= G41), R84 (= R64), N178 (= N175), N180 (= N177), A181 (= A178)

Sites not aligning to the query:

binding arginine: 270, 273, 275, 278, 280

4usjB N-acetylglutamate kinase from arabidopsis thaliana in complex with pii from chlamydomonas reinhardtii (see paper)
33% identity, 90% coverage: 8:258/278 of query aligns to 23:256/281 of 4usjB

query
sites
4usjB

I

V

K
|
K

I

Y

G

G

G
|
G

N

A

V

A

I

M

D

T

N

S

S

P

E

E

N

L

L

K

H

S

Q

S

F

V

L

V

L

S

D

D

F

L

T

V

A

L

L

L

P

A

G

C

-

V

-

G

-

L

D

R

K

P

I

I

L

L

V

V

H

H

G
|
G

K

P

I

D

A

I

T

N

E

R

L

Y

G

L

E

K

S

Q

L

L

G

N

I

I

E

P

A

A

K

E

M

F

I

R

D

D

G

G

R

L

R
|
R

I

V

T

T

D

D

I

A

E

T

T

T

L

M

R

E

I

I

V

V

T

S

M

M

V

V

Y

L

A

V

G

G

L

K

I

V

N

N

K

K

N

N

M

L

V

V

A

S

Q

L

L

I

Q

N

A

A

K

A

G

G

S

A

N

T

A

A

I

V

G

G

L

L

S

S

G

G

A

H

D

D

G

G

N

R

V

L

I

L

R

T

A

A

K

-

R

R

P

P

V

V

I

P

T

N

K

S

T

A

Y

-

G

-

A

-

E

-

S

-

G

-

P

-

M

-

A

-

G

-

A

-

V

-

I

-

D

Q

Y

L

G

G

F

F

V

V

G

G

D

E

L

V

D

A

E

R

N

-

A

-

V

V

S

D

S

P

T

S

T

V

L

L

E

R

S

P

L

L

L

V

K

D

A

Y

G

G

L

Y

V

I

P

P

V

V

L

I

C

A

A

S

I

V

T

A

H

A

D

D

G

D

D

S

T

G

Q

Q

L

A

L

Y

N
|
N

T

I

N

N

A
|
A

D
|
D

T

T

I

V

A

A

S

G

S

E

V

L

A

A

V

A

A

A

M

L

S

G

A

A

L

-

Y

-

E

-

T

E

R
x
K

L

L

V

I

Y

L

C

L

F

T

E

D

K

V

K

A

G

G

V

I

L

L

K

E

N

N

V

K

N

E

D

D

D

P

G

S

S

S

V

L

V

I

R
x
K

E

E

I

I

K

D

A

I

D

K

E

G

F

V

E

K

G

K

L

M

K

I

A

E

D

D

G

G

T

K

V

V

Q

A

G

G

M

M

I

I

P

P

K
|
K

L

V

H

K

N

C

A

C

F

I

E

R

A

S

I

L

K

A

K

Q

G

G

V

V

S

K

A

T

V

A

Y

S

I

I

active site: K25 (= K10), G28 (= G13), G61 (= G43), D180 (= D179), K239 (= K241)
binding arginine: K194 (≠ R196), K216 (≠ R218)
binding n-acetyl-l-glutamyl 5-phosphate: G59 (= G41), G60 (= G42), G61 (= G43), R82 (= R64), N176 (= N175), A179 (= A178)

Sites not aligning to the query:

binding arginine: 17, 268, 269, 271, 273, 274, 278

3wwmA Crystal structure of lysz from thermus thermophilus with adp (see paper)
35% identity, 81% coverage: 37:261/278 of query aligns to 32:251/269 of 3wwmA

query
sites
3wwmA

I

L

L

L

V

V

H

H

G
|
G

G
x
S

K

A

I

E

A

T

T

N

E

K

L

V

G

A

E

E

S

A

L

L

G

G

I

H

E

P

A

P

K

R

M

F

I

L

D

T

G

H

-

P

-

G

-

Q

-

V

R

S

R

R

I

L

T

T

D

D

I

R

E

K

T

T

L

L

R

E

I

I

V

F

T

E

M

M

V

V

Y
|
Y

A

C

G

G

L

L

I

V

N

N

K

K

N

R

M

L

V

V

A

E

Q

L

L

L

Q

Q

A

K

K

E

G

G

S

A

N

N

A

A

I

I

G

G

L

L

S

S

G

G

A

L

D

D

G

G

N

R

V

L

I

F

R

V

A

G

K

R

R

K

P

-

V

-

I

-

T

T

K

A

T

V

Y

K

G

Y

A

V

E

E

S

N

G

G

P

K

M

V

A

K

G

V

A

H

V

R

I

G

D

D

Y

Y

G

-

F

-

V

T

G

G

D

T

L

V

D

E

E

E

N

-

A

-

V

V

S

N

S

K

T

A

T

L

L

L

E

D

S

L

L

L

K

Q

A

A

G

G

L

Y

V

L

P

P

V

V

L

L

C

T

A

P

I

P

T

A

H

L

D

S

G

Y

D

E

T

N

Q

E

L

A

L

I

N

N

T

T

N

D

A

G

D
|
D

T

Q

I

I

A

A

S

A

S

L

V

L

A

A

V

T

A

L

M

Y

S

G

A

A

L

-

Y

-

E

-

T

E

R

A

L

L

V

V

Y

Y

C

L

F

S

E

N

K

V

K

P

G

G

V

L

L

L

K

A

N

R

V
x
Y

N

P

D

D

D

E

G

A

S

S

V

L

V

V

R

R

E

E

I

I

K

P

A

V

D

E

E

R

F

I

E

E

G

D

L

P

K

E

A

Y

D

L

G

A

T

L

V

A

Q

Q

G

G

G

R

M
|
M

I

K

P

R

K
|
K

L

V

H

M

N

G

A

A

F

V

E

E

A

A

I

V

K

R

K

G

G

G

V

V

S

K

A

R

V

V

Y

V

I

F

G

A

K

D

A

A

active site: S38 (≠ G43), D172 (= D179), K231 (= K241)
binding adenosine-5'-diphosphate: G36 (= G41), G37 (= G42), S38 (≠ G43), Y78 (= Y78), Y200 (≠ V210), M228 (= M238)

Sites not aligning to the query:

active site: 5, 8
binding adenosine-5'-diphosphate: 8

O50147 [LysW]-aminoadipate kinase; EC 2.7.2.17 from Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27) (see paper)
35% identity, 81% coverage: 37:261/278 of query aligns to 32:251/269 of O50147

query
sites
O50147

I

L

L

L

V

V

H

H

G

G

G

S

K

A

I

E

A

T

T

N

E

K

L

V

G

A

E

E

S

A

L

L

G

G

I

H

E

P

A

P

K

R

M

F

I

L

D

T

G
x
H

-

P

-

G

-

Q

-

V

R

S

R

R

I

L

T

T

D

D

I

R

E

K

T

T

L

L

R

E

I

I

V

F

T

E

M

M

V

V

Y

Y

A

C

G

G

L

L

I

V

N

N

K

K

N

R

M

L

V

V

A

E

Q

L

L

L

Q

Q

A

K

K

E

G

G

S

A

N

N

A

A

I

I

G

G

L

L

S

S

G

G

A

L

D

D

G

G

N

R

V

L

I

F

R

V

A

G

K
x
R

R
x
K

P

-

V

-

I

-

T

T

K

A

T

V

Y
x
K

G

Y

A

V

E

E

S

N

G

G

P
x
K

M

V

A
x
K

G

V

A

H

V
x
R

I

G

D

D

Y

Y

G

-

F

-

V

T

G

G

D

T

L

V

D

E

E

E

N

-

A

-

V

V

S

N

S

K

T

A

T

L

L

L

E

D

S

L

L

L

K

Q

A

A

G

G

L

Y

V

L

P

P

V

V

L

L

C

T

A

P

I

P

T

A

H

L

D

S

G

Y

D

E

T

N

Q

E

L

A

L

I

N

N

T

T

N

D

A

G

D

D

T

Q

I

I

A

A

S

A

S

L

V

L

A

A

V

T

A

L

M

Y

S

G

A

A

L

-

Y

-

E

-

T

E

R

A

L

L

V

V

Y

Y

C

L

F

S

E

N

K

V

K

P

G

G

V

L

L

L

K

A

N

R

V

Y

N

P

D

D

D

E

G

A

S

S

V

L

V

V

R

R

E

E

I

I

K

P

A

V

D

E

E

R

F

I

E

E

G

D

L

P

K

E

A

Y

D

L

G

A

T

L

V

A

Q

Q

G

G

G
x
R

M

M

I

K

P
x
R

K
|
K

L

V

H

M

N

G

A

A

F

V

E

E

A

A

I

V

K

R

K

G

G

G

V

V

S

K

A

R

V

V

Y

V

I

F

G

A

K

D

A

A

H57 (≠ G62) mutation to A: Shows reduced activity.
R111 (≠ K111) mutation to E: Shows reduced activity.
R112 (≠ K112) mutation to E: Shows reduced activity.
K113 (≠ R113) mutation to E: Loss of activity.
K117 (≠ Y120) mutation to E: Does not bind LysW.
K123 (≠ P126) mutation to E: Does not bind LysW.
K125 (≠ A128) mutation to E: Does not bind LysW.
R128 (≠ V131) mutation to E: Does not bind LysW.
R227 (≠ G237) mutation to E: Still binds LysW, but shows reduced activity.
R230 (≠ P240) mutation to E: Still binds LysW, but shows reduced activity.
K231 (= K241) mutation to E: Still binds LysW, but shows reduced activity.

2bufK Arginine feed-back inhibitable acetylglutamate kinase (see paper)
31% identity, 90% coverage: 8:258/278 of query aligns to 30:248/273 of 2bufK

query
sites
2bufK

I

V

I

I

K
|
K

I

Y

G

G

G
|
G

N

N

V

A

I

M

D

E

N

S

S

E

E

E

N

L

L

K

H

A

Q

G

F

F

L

A

L

R

D

D

F

V

T

V

A

L

L

M

P

K

G

A

-

V

-

G

-

I

D

N

K

P

I

V

L

V

V

V

H

H

G

G

G
|
G

K

P

I

Q

A

I

T

G

E

D

L

L

G

L

E

K

S

R

L

L

G

S

I

I

E

E

A

S

K

-

M

-

I

-

D

-

G

-

R

H

R
|
R

I

V

T

T

D

D

I

A

E

A

T

T

L

M

R

D

I

V

V

V

T

E

M

M

V

V

Y

L

A

G

G

G

L

Q

I

V

N

N

K

K

N

D

M

I

V

V

A

N

Q

L

L

I

Q

N

A

R

K

H

G

G

S

G

N

S

A

A

I

I

G

G

L

L

S

T

G

G

A

K

D

D

G

A

N

E

V

L

I

I

R

R

A

A

K

K

R

L

P

-

V

-

I

-

T

-

K

-

T

T

Y

V

G

G

A

E

E

V

S

T

G

G

P

-

M

-

A

-

G

-

A

-

V

-

I

-

D

-

Y

-

G

-

F

-

V

-

G

-

D

-

L

-

D

-

E

-

N

-

A

-

V

V

S

N

S

V

T

G

T

L

L

L

E

N

S

M

L

L

L

V

K

K

A

G

G

D

L

F

V

I

P

P

V

V

L

I

C

A

A

P

I

I

T

G

H

V

D

G

G

S

D

N

T

G

Q

E

L

S

L

Y

N
|
N

T

I

N
|
N

A
|
A

D
|
D

T

L

I

V

A

A

S

G

S

K

V

V

A

A

V

E

A

A

M

L

S

K

A

A

L

-

Y

-

E

-

T

-

R

-

L

-

V

-

Y

-

C

-

F

-

E

E

K

K

K

L

G

M

V

L

L

L

K

T

N

N

V

I

-

A

-

G

-

L

-

M

N

D

D

K

D

Q

G

G

S

Q

V

V

V

L

R

T

E

G

I

L

K

S

A

T

D

E

E

Q

F

V

E

N

G

E

L

L

K

I

A

A

D

D

G

G

T

T

V

I

Q

Y

G

G

M

M

I

L

P

P

K
|
K

L

I

H

R

N

C

A

A

F

L

E

E

A

A

I

V

K

Q

K

G

G

G

V

V

S

T

A

S

V

A

Y

H

I

I

active site: K32 (= K10), G35 (= G13), G68 (= G43), D175 (= D179), K231 (= K241)
binding n-acetyl-l-glutamate: R84 (= R64), N171 (= N175), N173 (= N177), A174 (= A178)

7nnbA Crystal structure of mycobacterium tuberculosis argb in complex with 2,8-bis(trifluoromethyl)quinolin-4-ol.
28% identity, 91% coverage: 5:258/278 of query aligns to 26:265/291 of 7nnbA

query
sites
7nnbA

Q

K

L

V

T

V

I

V

K

K

I

Y

G

G

N

N

V

A

I

M

D

T

N

D

S

D

E

T

N

L

L

R

H

R

Q

A

F

F

L

A

D

D

F

M

T

A

A

F

L

L

P

R

G

N

-

C

-

G

-

I

D

H

K

P

I

V

L

V

V

V

H

H

G

G

K

P

I

Q

A

I

T

T

E

A

L

M

G

L

E

R

S

R

L

L

G

G

I

I

E

E

A

G

K

D

M

F

I

K

D

G

G

G

R

F

R

R

I

V

T

T

D

T

I

P

E

E

T

V

L

L

R

D

I

V

V

A

T

R

M

M

V

V

Y

L

A

F

G

G

L

Q

I

V

N

G

K

R

N

E

M

L

V

V

A

N

Q

L

L

I

Q

N

A

A

K

H

G

G

S

P

N

Y

A

A

I
x
V

G

G

L

I

S

T

G

G

A

E

D

D

G

A

N

Q

V

L

I

F

R

T

A

A

K

V

K

R

R

R

P

S

V

V

I

T

T

V

K

D

T

-

Y

-

G

-

A

-

E

-

S

-

G

-

P

-

M

-

A

-

G

G

A

V

V

A

I

T

D

D

Y

I

G

G

F

L

V

V

G

G

D

D

L

V

D

D

E

Q

N

-

A

-

V

V

S

N

S

T

T

A

L

M

E

L

S

D

L
|
L

L

V

K

A

A

A

G

G

L

R

V

I

P

P

V

V

L

V

C

S

A

T

I

L

T

A

H

P

D

D

G

A

D

D

T

G

Q

V

L

V

L

H

N

N

T

I

N

N

A

A

D

-

T

-

I

-

A

D

S

T

S

A

V

A

A

A

V

A

A

V

M

A

S

E

A

A

L

L

Y

G

E

A

T

E

R

K

L

L

V

L

Y

M

C

L

F

T

E

D

K

I

K

D

G

G

V

L

L

Y

K

T

N

R

V

W

N

P

D

D

D

R

G

D

S

S

V

L

V

V

R

S

E

E

I

I

K

D

A

T

D

G

E

T

F

L

E

A

G

Q

L

L

K

L

A

P

D

-

G

-

T

T

V

L

Q

E

G

S

G

G

M

M

I

V

P

P

K

K

L

V

H

E

N

A

A

C

F

L

E

R

A

A

I

V

K

I

K

G

G

G

V

V

S

P

A

S

V

A

Y

H

I

I

binding 2,8-bis(trifluoromethyl)quinolin-4-ol: V122 (≠ I98), L165 (= L153)

7nn8A Crystal structure of mycobacterium tuberculosis argb in complex with 1h-indole-3-carbonitrile.
28% identity, 91% coverage: 5:258/278 of query aligns to 26:265/291 of 7nn8A

query
sites
7nn8A

Q

K

L

V

T

V

I

V

K

K

I

Y

G

G

N

N

V

A

I

M

D

T

N

D

S

D

E

T

N

L

L

R

H

R

Q

A

F

F

L

A

D

D

F

M

T

A

A

F

L

L

P

R

G

N

-

C

-

G

-

I

D

H

K

P

I

V

L

V

V

V

H

H

G

G

K

P

I

Q

A

I

T

T

E

A

L

M

G

L

E

R

S

R

L

L

G

G

I

I

E

E

A

G

K

D

M

F

I

K

D

G

G

G

R

F

R

R

I

V

T

T

D

T

I

P

E

E

T

V

L

L

R

D

I

V

V

A

T

R

M

M

V

V

Y

L

A

F

G

G

L

Q

I

V

N

G

K

R

N

E

M

L

V

V

A

N

Q

L

L

I

Q

N

A

A

K

H

G

G

S

P

N

Y

A

A

I
x
V

G

G

L

I

S

T

G

G

A

E

D

D

G

A

N

Q

V

L

I

F

R

T

A

A

K

V

K

R

R

R

P

S

V

V

I

T

T

V

K

D

T

-

Y

-

G

-

A

-

E

-

S

-

G

-

P

-

M

-

A

-

G

G

A

V

V

A

I

T

D

D

Y

I

G

G

F

L

V

V

G

G

D

D

L

V

D

D

E

Q

N

-

A

-

V

V

S

N

S

T

T

A

L

M

E

L

S

D

L
|
L

L

V

K

A

A

A

G

G

L

R

V

I

P

P

V

V

L

V

C

S

A

T

I

L

T

A

H

P

D

D

G

A

D

D

T

G

Q

V

L

V

L

H

N

N

T

I

N

N

A

A

D

-

T

-

I

-

A

D

S

T

S

A

V

A

A

A

V

A

A

V

M

A

S

E

A

A

L

L

Y

G

E

A

T

E

R

K

L

L

V

L

Y

M

C

L

F

T

E

D

K

I

K

D

G

G

V

L

L

Y

K

T

N

R

V

W

N

P

D

D

D

R

G

D

S

S

V

L

V

V

R

S

E

E

I

I

K

D

A

T

D

G

E

T

F

L

E

A

G

Q

L

L

K

L

A

P

D

-

G

-

T

T

V

L

Q

E

G

S

G

G

M

M

I

V

P

P

K

K

L

V

H

E

N

A

A

C

F

L

E

R

A

A

I

V

K

I

K

G

G

G

V

V

S

P

A

S

V

A

Y

H

I

I

binding 1~{H}-indole-3-carbonitrile: V122 (≠ I98), L165 (= L153)

7nm0A Crystal structure of mycobacterium tuberculosis argb in complex with 1-(2,6-dihydroxyphenyl)ethan-1-one.
28% identity, 91% coverage: 5:258/278 of query aligns to 26:265/291 of 7nm0A

query
sites
7nm0A

Q

K

L

V

T

V

I

V

K

K

I

Y

G

G

N

N

V

A

I

M

D

T

N

D

S

D

E

T

N

L

L

R

H

R

Q

A

F

F

L

A

D

D

F

M

T

A

A

F

L

L

P

R

G

N

-

C

-

G

-

I

D

H

K

P

I

V

L

V

V

V

H

H

G

G

K

P

I

Q

A

I

T

T

E

A

L

M

G

L

E

R

S

R

L

L

G

G

I

I

E

E

A

G

K

D

M

F

I

K

D

G

G

G

R

F

R

R

I

V

T

T

D

T

I

P

E

E

T

V

L

L

R

D

I

V

V

A

T

R

M

M

V

V

Y

L

A

F

G

G

L

Q

I

V

N

G

K

R

N

E

M

L

V

V

A

N

Q

L

L

I

Q

N

A

A

K

H

G

G

S

P

N

Y

A

A

I
x
V

G

G

L

I

S

T

G

G

A

E

D

D

G

A

N

Q

V

L

I

F

R

T

A

A

K

V

K

R

R

R

P

S

V

V

I

T

T

V

K

D

T

-

Y

-

G

-

A

-

E

-

S

-

G

-

P

-

M

-

A

-

G

G

A

V

V

A

I

T

D

D

Y

I

G

G

F

L

V

V

G

G

D

D

L

V

D

D

E

Q

N

-

A

-

V

V

S

N

S

T

T

A

L

M

E

L

S

D

L
|
L

L

V

K

A

A

A

G

G

L

R

V

I

P

P

V

V

L

V

C

S

A

T

I

L

T

A

H

P

D

D

G

A

D

D

T

G

Q

V

L

V

L

H

N

N

T

I

N

N

A

A

D

-

T

-

I

-

A

D

S

T

S

A

V

A

A

A

V

A

A

V

M

A

S

E

A

A

L

L

Y

G

E

A

T

E

R

K

L

L

V

L

Y

M

C

L

F

T

E

D

K

I

K

D

G

G

V

L

L

Y

K

T

N

R

V

W

N

P

D

D

D

R

G

D

S

S

V

L

V

V

R

S

E

E

I

I

K

D

A

T

D

G

E

T

F

L

E

A

G

Q

L

L

K

L

A

P

D

-

G

-

T

T

V

L

Q

E

G

S

G

G

M

M

I

V

P

P

K

K

L

V

H

E

N

A

A

C

F

L

E

R

A

A

I

V

K

I

K

G

G

G

V

V

S

P

A

S

V

A

Y

H

I

I

binding 1-[2,6-bis(oxidanyl)phenyl]ethanone: V122 (≠ I98), L165 (= L153)

7nlzA Crystal structure of mycobacterium tuberculosis argb in complex with 5-methoxy-6-(trifluoromethyl)indole.
28% identity, 91% coverage: 5:258/278 of query aligns to 26:265/291 of 7nlzA

query
sites
7nlzA

Q

K

L

V

T

V

I

V

K

K

I

Y

G

G

N

N

V

A

I

M

D

T

N

D

S

D

E

T

N

L

L

R

H

R

Q

A

F

F

L

A

D

D

F

M

T

A

A

F

L

L

P

R

G

N

-

C

-

G

-

I

D

H

K

P

I

V

L

V

V

V

H

H

G

G

K

P

I

Q

A

I

T

T

E

A

L

M

G

L

E

R

S

R

L

L

G

G

I

I

E

E

A

G

K

D

M

F

I

K

D

G

G

G

R

F

R

R

I

V

T

T

D

T

I

P

E

E

T

V

L

L

R

D

I

V

V

A

T

R

M

M

V

V

Y

L

A

F

G

G

L

Q

I

V

N

G

K

R

N

E

M

L

V

V

A

N

Q

L

L

I

Q

N

A

A

K

H

G

G

S

P

N

Y

A

A

I
x
V

G

G

L

I

S

T

G

G

A

E

D

D

G

A

N

Q

V

L

I

F

R

T

A

A

K

V

K

R

R

R

P

S

V

V

I

T

T

V

K

D

T

-

Y

-

G

-

A

-

E

-

S

-

G

-

P

-

M

-

A

-

G

G

A

V

V

A

I

T

D

D

Y

I

G

G

F

L

V

V

G

G

D

D

L

V

D

D

E

Q

N

-

A

-

V

V

S

N

S

T

T

A

L

M

E

L

S

D

L
|
L

L

V

K

A

A

A

G

G

L

R

V

I

P

P

V

V

L

V

C

S

A

T

I

L

T

A

H

P

D

D

G

A

D

D

T

G

Q

V

L

V

L

H

N

N

T

I

N

N

A

A

D

-

T

-

I

-

A

D

S

T

S

A

V

A

A

A

V

A

A

V

M

A

S

E

A

A

L

L

Y

G

E

A

T

E

R

K

L

L

V

L

Y

M

C

L

F

T

E

D

K

I

K

D

G

G

V

L

L

Y

K

T

N

R

V

W

N

P

D

D

D

R

G

D

S

S

V

L

V

V

R

S

E

E

I

I

K

D

A

T

D

G

E

T

F

L

E

A

G

Q

L

L

K

L

A

P

D

-

G

-

T

T

V

L

Q

E

G

S

G

G

M

M

I

V

P

P

K

K

L

V

H

E

N

A

A

C

F

L

E

R

A

A

I

V

K

I

K

G

G

G

V

V

S

P

A

S

V

A

Y

H

I

I

binding 5-methoxy-6-(trifluoromethyl)-1~{H}-indole: V122 (≠ I98), L165 (= L153)

7nltA Crystal structure of mycobacterium tuberculosis argb in complex with 4-(4-methylpiperazin-1-yl)benzoic acid
28% identity, 91% coverage: 5:258/278 of query aligns to 26:265/291 of 7nltA

query
sites
7nltA

Q

K

L

V

T

V

I

V

K

K

I

Y

G

G

N

N

V

A

I

M

D

T

N

D

S

D

E

T

N

L

L

R

H

R

Q

A

F

F

L

A

D

D

F

M

T

A

A

F

L

L

P

R

G

N

-

C

-

G

-

I

D

H

K

P

I

V

L

V

V

V

H

H

G

G

K

P

I

Q

A

I

T

T

E

A

L

M

G

L

E

R

S

R

L

L

G

G

I

I

E

E

A

G

K

D

M

F

I

K

D

G

G

G

R

F

R

R

I

V

T

T

D

T

I

P

E

E

T

V

L

L

R

D

I

V

V

A

T

R

M

M

V

V

Y

L

A

F

G

G

L

Q

I

V

N

G

K

R

N

E

M

L

V

V

A

N

Q

L

L

I

Q

N

A

A

K

H

G

G

S

P

N

Y

A

A

I
x
V

G

G

L

I

S

T

G

G

A

E

D

D

G

A

N

Q

V
x
L

I

F

R

T

A

A

K

V

K

R

R

R

P

S

V

V

I

T

T

V

K

D

T

-

Y

-

G

-

A

-

E

-

S

-

G

-

P

-

M

-

A

-

G

G

A

V

V

A

I

T

D

D

Y

I

G

G

F

L

V

V

G

G

D

D

L

V

D

D

E

Q

N

-

A

-

V

V

S

N

S

T

T

A

L

M

E

L

S

D

L
|
L

L

V

K

A

A

A

G

G

L

R

V

I

P

P

V

V

L

V

C

S

A

T

I

L

T

A

H

P

D

D

G

A

D

D

T

G

Q

V

L

V

L

H

N

N

T

I

N

N

A

A

D

-

T

-

I

-

A

D

S

T

S

A

V

A

A

A

V

A

A

V

M

A

S

E

A

A

L

L

Y

G

E

A

T

E

R

K

L

L

V

L

Y

M

C

L

F

T

E

D

K

I

K

D

G

G

V

L

L

Y

K

T

N

R

V

W

N

P

D

D

D

R

G

D

S

S

V

L

V

V

R

S

E

E

I

I

K

D

A

T

D

G

E

T

F

L

E

A

G

Q

L

L

K

L

A

P

D

-

G

-

T

T

V

L

Q

E

G

S

G

G

M

M

I

V

P

P

K

K

L

V

H

E

N

A

A

C

F

L

E

R

A

A

I

V

K

I

K

G

G

G

V

V

S

P

A

S

V

A

Y

H

I

I

binding 4-(4-methylpiperazin-1-yl)benzoic acid: V122 (≠ I98), L131 (≠ V107), L165 (= L153)

7nlrA Crystal structure of mycobacterium tuberculosis argb in complex with 2-phenyl-1h-imidazole
28% identity, 91% coverage: 5:258/278 of query aligns to 26:265/291 of 7nlrA

query
sites
7nlrA

Q

K

L

V

T

V

I

V

K

K

I

Y

G

G

N

N

V

A

I

M

D

T

N

D

S

D

E

T

N

L

L

R

H

R

Q

A

F

F

L

A

D

D

F

M

T

A

A

F

L

L

P

R

G

N

-

C

-

G

-

I

D

H

K

P

I

V

L

V

V

V

H

H

G

G

K

P

I

Q

A

I

T

T

E

A

L

M

G

L

E

R

S

R

L

L

G

G

I

I

E

E

A

G

K

D

M

F

I

K

D

G

G

G

R

F

R

R

I

V

T

T

D

T

I

P

E

E

T

V

L

L

R

D

I

V

V

A

T

R

M

M

V

V

Y

L

A

F

G

G

L

Q

I

V

N

G

K

R

N

E

M

L

V

V

A

N

Q

L

L

I

Q

N

A

A

K

H

G

G

S

P

N

Y

A

A

I
x
V

G

G

L
x
I

S

T

G

G

A

E

D

D

G

A

N

Q

V

L

I

F

R

T

A

A

K

V

K

R

R

R

P

S

V

V

I

T

T

V

K

D

T

-

Y

-

G

-

A

-

E

-

S

-

G

-

P

-

M

-

A

-

G

G

A

V

V

A

I

T

D

D

Y

I

G

G

F

L

V

V

G

G

D

D

L

V

D

D

E

Q

N

-

A

-

V

V

S

N

S

T

T

A

L

M

E

L

S

D

L
|
L

L

V

K

A

A

A

G

G

L

R

V

I

P

P

V

V

L

V

C

S

A

T

I

L

T

A

H

P

D

D

G

A

D

D

T

G

Q

V

L

V

L

H

N

N

T

I

N

N

A

A

D

-

T

-

I

-

A

D

S

T

S

A

V

A

A

A

V

A

A

V

M

A

S

E

A

A

L

L

Y

G

E

A

T

E

R

K

L

L

V

L

Y

M

C

L

F

T

E

D

K

I

K

D

G

G

V

L

L

Y

K

T

N

R

V

W

N

P

D

D

D

R

G

D

S

S

V

L

V

V

R

S

E

E

I

I

K

D

A

T

D

G

E

T

F

L

E

A

G

Q

L

L

K

L

A

P

D

-

G

-

T

T

V

L

Q

E

G

S

G

G

M

M

I

V

P

P

K

K

L

V

H

E

N

A

A

C

F

L

E

R

A

A

I

V

K

I

K

G

G

G

V

V

S

P

A

S

V

A

Y

H

I

I

binding 2-phenyl-1H-imidazole: V122 (≠ I98), I124 (≠ L100), L165 (= L153)

7nloA Crystal structure of mycobacterium tuberculosis argb in complex with l-arginine
28% identity, 91% coverage: 5:258/278 of query aligns to 24:263/289 of 7nloA

query
sites
7nloA

Q

K

L

V

T

V

I

V