SitesBLAST
Comparing CA265_RS19720 FitnessBrowser__Pedo557:CA265_RS19720 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
3dh4A Crystal structure of sodium/sugar symporter with bound galactose from vibrio parahaemolyticus (see paper)
47% identity, 92% coverage: 48:558/558 of query aligns to 21:508/512 of 3dh4A
Q9NY91 Probable glucose sensor protein SLC5A4; Solute carrier family 5 member 4 from Homo sapiens (Human) (see paper)
31% identity, 83% coverage: 1:463/558 of query aligns to 19:498/659 of Q9NY91
- E457 (≠ Q422) mutation to Q: Confers sugar transport activity not found in the wild-type protein. Increased sensitivity to inhibitor phlorizin.
7wmvA Structure of human sglt1-map17 complex bound with lx2761 (see paper)
31% identity, 80% coverage: 7:452/558 of query aligns to 8:470/602 of 7wmvA
- binding N-[2-(dimethylamino)ethyl]-2-methyl-2-[4-[4-[[2-methyl-5-[(2S,3R,4R,5S,6R)-6-methylsulfanyl-3,4,5-tris(oxidanyl)oxan-2-yl]phenyl]methyl]phenyl]butanoylamino]propanamide: N61 (= N62), H66 (≠ Q67), L70 (≠ M71), I81 (= I82), F84 (≠ Y85), L257 (≠ I244), M266 (= M256), L269 (≠ V259), T270 (≠ N260), Y273 (= Y263), W274 (= W264), F436 (= F418), D437 (≠ Q419), Q440 (= Q422)
Sites not aligning to the query:
P13866 Sodium/glucose cotransporter 1; Na(+)/glucose cotransporter 1; High affinity sodium-glucose cotransporter; Solute carrier family 5 member 1 from Homo sapiens (Human) (see 7 papers)
31% identity, 80% coverage: 7:452/558 of query aligns to 25:487/664 of P13866
- N51 (≠ K33) to S: in GGM; slightly decreased activity; dbSNP:rs17683011
- W67 (= W51) mutation to A: Strong reduction in D-glucose transporter activity.
- S77 (= S61) mutation to A: Loss of activity.
- H83 (≠ Q67) mutation to L: Acquires D-mannose, D-fructose and L-sorbose transporter activity; when associated with A-287 and C-290.; mutation to Q: Loss of D-glucose transporter activity.
- R135 (= R119) to W: in GGM; loss of activity
- S159 (≠ T142) to P: in GGM; loss of activity; dbSNP:rs933026071
- A166 (= A149) to T: in GGM; about 90% reduction in activity
- D204 (= D186) mutation to A: Loss of activity.
- N248 (≠ Y221) modified: carbohydrate, N-linked (GlcNAc...) asparagine; mutation to Q: Loss of N-glycosylation.
- C255 (≠ A228) modified: Disulfide link with 511
- W276 (≠ L246) to L: in GGM; about 95% reduction in activity
- T287 (≠ N260) mutation to A: Acquires D-mannose, D-fructose and L-sorbose transporter activity; when associated with L-83 and C-290.; mutation to N: Loss of D-glucose transporter activity. Has strict selectivity for D-galactose.; mutation T->S,A: Has normal D-glucose and D-galactose transporter activity.
- Y290 (= Y263) mutation to C: Loss of D-galactose transporter activity. Has strict selectivity for D-glucose. Acquires D-mannose, D-fructose and L-sorbose transporter activity; when associated with A-287 and L-83.
- W291 (= W264) mutation to A: Loss of D-glucose transporter activity.
- C292 (≠ G265) to Y: in GGM; loss of activity; dbSNP:rs765502638; mutation to A: Has no effect on water permeability.
- Q295 (= Q268) to R: in GGM; loss of activity; dbSNP:rs779428134
- R300 (= R273) to S: in GGM; loss of activity
- A304 (= A277) to V: in GGM; impairs trafficking to the plasma membrane
- K321 (= K293) mutation to Q: Acquires D-mannose and D-allose transporter activity comparable to glucose and galactose.
- C345 (vs. gap) modified: Disulfide link with 351
- C351 (≠ S318) modified: Disulfide link with 345
- C355 (≠ Q322) modified: Disulfide link with 361
- C361 (≠ P328) modified: Disulfide link with 355
- N363 (≠ R330) mutation to A: Loss of water permeation.
- L369 (= L335) to S: in GGM; loss of activity
- R379 (≠ K344) to Q: in GGM; loss of activity; dbSNP:rs747215838
- A388 (= A353) to V: in GGM; loss of activity
- S396 (≠ G361) mutation to A: Loss of activity.
- F405 (= F370) to S: in GGM; loss of activity
- A411 (≠ K376) to T: in GGM; slightly decreased activity; dbSNP:rs17683430
- G426 (= G392) to R: in GGM; loss of activity; dbSNP:rs1304151494
- Q451 (≠ G417) mutation to A: Strong reduction in water permeation.
- L452 (vs. gap) mutation to A: Loss of water permeation.
- D454 (≠ Q419) mutation to A: Has no effect on water permeation.
- Q457 (= Q422) mutation to A: Loss of D-glucose transporter activity.; mutation to C: Strong reduction in D-glucose transporter activity.
- T460 (= T425) mutation to A: Loss of D-glucose transporter activity.
- V470 (≠ M435) to N: in GGM; about 90% reduction in activity; requires 2 nucleotide substitutions
Sites not aligning to the query:
- 191:664 natural variant: Missing (in GGM; loss of activity)
- 379:664 natural variant: Missing (in GGM; loss of activity)
- 499 R → H: in GGM; impairs trafficking to the plasma membrane; decreases the sugar affinity; dbSNP:rs927157864
- 511 modified: Disulfide link with 255
- 517 modified: Disulfide link with 522
- 522 modified: Disulfide link with 517
- 615 H → Q: in GGM; slightly decreased activity; dbSNP:rs33954001
- 641 W→A: Slightly reduced D-glucose transporter activity.
- 660:661 HA→WG: Loss of D-glucose transporter activity.
7sl8A Cryoem structure of sglt1 at 3.4 a resolution (see paper)
31% identity, 80% coverage: 7:452/558 of query aligns to 6:455/582 of 7sl8A
Sites not aligning to the query:
7slaA Cryoem structure of sglt1 at 3.15 angstrom resolution (see paper)
31% identity, 80% coverage: 7:452/558 of query aligns to 7:456/585 of 7slaA
Sites not aligning to the query:
Q9ET37 Solute carrier family 5 member 4A; SGLT3-a from Mus musculus (Mouse) (see paper)
30% identity, 83% coverage: 3:463/558 of query aligns to 21:498/656 of Q9ET37
- E457 (≠ Q422) mutation to Q: Confers sodium-dependent sugar transport activity not found in the wild type protein.
8hdhA Structure of human sglt2-map17 complex with canagliflozin (see paper)
32% identity, 80% coverage: 7:452/558 of query aligns to 2:467/586 of 8hdhA
- binding (2~{S},3~{R},4~{R},5~{S},6~{R})-2-[3-[[5-(4-fluorophenyl)thiophen-2-yl]methyl]-4-methyl-phenyl]-6-(hydroxymethyl)oxane-3,4,5-triol: N55 (= N62), G59 (≠ E66), H60 (≠ Q67), G63 (= G70), L64 (≠ M71), F78 (≠ Y85), E79 (= E86), S267 (≠ N260), W271 (= W264), F433 (= F418), D434 (≠ Q419), Q437 (= Q422)
- binding sodium ion: A53 (= A60), S54 (= S61), I56 (= I63), G57 (≠ S64), A369 (= A354), S372 (≠ A357), S373 (= S358)
Sites not aligning to the query:
- binding (2~{S},3~{R},4~{R},5~{S},6~{R})-2-[3-[[5-(4-fluorophenyl)thiophen-2-yl]methyl]-4-methyl-phenyl]-6-(hydroxymethyl)oxane-3,4,5-triol: 506
- binding : 575, 579, 580, 583, 584
8hb0A Structure of human sglt2-map17 complex with ta1887 (see paper)
32% identity, 80% coverage: 7:452/558 of query aligns to 2:467/586 of 8hb0A