SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing CA265_RS19780 CA265_RS19780 aldehyde dehydrogenase family protein to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

6dbbA Crystal structure of a putative aldehyde dehydrogenase family protein burkholderia cenocepacia j2315 in complex with partially reduced nadh
56% identity, 95% coverage: 25:513/513 of query aligns to 16:504/504 of 6dbbA

query
sites
6dbbA

S

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active site: N152 (= N165), E259 (= E266), C293 (= C300), E471 (= E480)
binding nicotinamide-adenine-dinucleotide: I148 (= I161), S149 (= S162), A150 (= A163), F151 (= F164), N152 (= N165), K175 (= K188), P176 (= P189), S177 (= S190), E178 (= E191), R218 (= R225), G221 (= G228), T236 (= T243), G237 (= G244), S238 (= S245), M241 (= M248), V245 (= V252), E259 (= E266), L260 (= L267), G261 (= G268), C293 (= C300), E391 (= E400), F393 (= F402), F460 (= F469)
binding beta-6-hydroxy-1,4,5,6-tetrhydronicotinamide adenine dinucleotide: I148 (= I161), S149 (= S162), A150 (= A163), F151 (= F164), N152 (= N165), K175 (= K188), P176 (= P189), S177 (= S190), E178 (= E191), R218 (= R225), G221 (= G228), T236 (= T243), G237 (= G244), S238 (= S245), M241 (= M248), V245 (= V252), E259 (= E266), L260 (= L267), G261 (= G268), C293 (= C300), E391 (= E400), F393 (= F402), F460 (= F469)

2jg7A Crystal structure of seabream antiquitin and elucidation of its substrate specificity (see paper)
54% identity, 98% coverage: 7:511/513 of query aligns to 11:509/509 of 2jg7A

query
sites
2jg7A

S

S

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W

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F

active site: N166 (= N165), K189 (= K188), E267 (= E266), C301 (= C300), E398 (= E400), E478 (= E480)
binding nicotinamide-adenine-dinucleotide: I162 (= I161), T163 (≠ S162), A164 (= A163), F165 (= F164), N166 (= N165), K189 (= K188), G190 (≠ P189), A191 (≠ S190), P192 (≠ E191), G225 (≠ D224), A226 (≠ R225), G229 (= G228), T230 (≠ E229), F243 (≠ A242), T244 (= T243), G245 (= G244), S246 (= S245), V249 (≠ M248), V253 (= V252), E267 (= E266), L268 (= L267), G269 (= G268), C301 (= C300), E398 (= E400), F400 (= F402), F467 (= F469)

4zulA Structure aldh7a1 complexed with alpha-aminoadipate (see paper)
54% identity, 98% coverage: 9:512/513 of query aligns to 12:509/509 of 4zulA

query
sites
4zulA

L

L

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W

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D

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N

D

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A

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C

G

G

D

G

R

A

E

D

V

I

G

G

E

T

R

A

M

M

T

A

N

K

D

D

G

E

R

R

I

V

P

N

L

L

I

L

S

S

A

F

T

T

G

G

S

S

T

T

R

Q

M

V

G

G

K

K

A

Q

V

V

G

G

A

L

A

M

V

V

G

Q

A

E

R

R

L

F

G

G

K

R

S

S

L

L

E
|
E

L

L

G

G

N

N

A

A

I

I

I

A

S

F

E

E

H

D

A

A

D

D

L

L

D

S

M

L

S

V

L

V

I

P

G

S

A

A

V

L

F

F

G

A

A

A

V

V

G

G

T

T

A

A

G

G

Q

Q

R
|
R

C
|
C

T
|
T

S

T

T

A

R

R

L

L

I

F

I

I

H

H

E

E

S

S

V

I

Y

H

D

D

A

E

F

V

T

V

A

N

K

R

L

L

V

K

K

K

A

A

Y

Y

G

A

Q

Q

L

I

R

R

I

V

G

G

D

N

P

P

L

W

D

D

Q

P

N

N

N

V

H

L

V

Y

G

G

P

P

L

L

I

H

D

T

T

K

D

Q

A

A

V

V

A

S

A

M

Y

F

L

L

D

G

S

A

I

V

A

E

K

E

C

A

K

K

A

K

E

E

G

G

N

T

F

V

V

V

V

Y

E

G

G

G

G

K

V

V

L

M

S

D

G

-

D

-

A

-

Y

-

T

R

S

P

G

G

C

N

Y

Y

V

V

K

E

P

P

C

T

I

I

A

V

E

T

-

G

V

L

Q

G

N

H

D

D

F

A

K

S

I

I

V

A

Q

H

H

T

E
|
E

T

T

F

F

A

A

P

P

I

I

L

L

Y

Y

L

V

I

F

K

K

Y

F

K

K

T

N

L

E

D

E

E

E

A

V

I

F

A

A

L

W

Q

N

N

N

G

E

V

V

P

K

Q

Q

G

G

L

L

S

S

A

S

I

I

M

F

T

T

L

K

N

D

L

L

R

G

E

R

A

I

E

F

Q

R

F

W

L

L

S

G

A

P

K

K

G

G

S

S

D

D

C

C

G

G

I

I

A

V

N

N

V

V

N

N

I

I

G

P

T

T

S
|
S

G
|
G

A
|
A

E

E

I

I

G

G

A

A

F
|
F

G

G

E

E

K

K

E

H

T

T

G

G

R

R

E
|
E

S

S

G

G

S

S

D

D

A

A

W

W

R

K

A

Q

Y

Y

M

M

R

R

Q

S

T

T

N

C

T

T

I

I

N

N

Y

Y

S

S

N

K

T

D

L

L

P

P

L

L

A

A

Q

Q

G

G

I

I

K

K

F

F

D

Q

active site: N165 (= N165), K188 (= K188), E266 (= E266), C300 (= C300), E397 (= E400), E477 (= E480)
binding 2-aminohexanedioic acid: E119 (= E119), N165 (= N165), F166 (= F166), W173 (= W173), R299 (= R299), C300 (= C300), T301 (= T301), S458 (= S461), G459 (= G462), A460 (= A463), F466 (= F469)

4x0tA Structure aldh7a1 inactivated by 4-diethylaminobenzaldehyde and complexed with NAD+ (see paper)
54% identity, 98% coverage: 9:512/513 of query aligns to 12:509/509 of 4x0tA

query
sites
4x0tA

L

L

N

K

K

E

L

L

G

G

I

L

N

R

A

E

S

E

N

N

A

E

A

G

Y

V

S

Y

T

N

G

G

S

S

N

-

W

W

G

G

E

R

L

G

N

E

V

V

N

I

T

T

L

-

E

-

S

T

F

Y

S

C

P

P

V

A

D

N

G

N

K

E

L

P

I

I

A

A

S

R

A

V

K

R

I

Q

A

A

T

S

A

V

D

A

D

D

Y

Y

D

E

A

E

V

T

V

V

L

K

K

K

A

A

Q

R

E

E

A

A

F

W

T

K

A

I

W

W

R

A

S

D

V

I

P

P

A

A

P

P

K

K

R

R

G

G

E

E

I

I

V

V

R

R

Q

Q

F

I

G

G

D

D

A

A

L

L

R

R

E

E

N

K

K

I

D

Q

A

V

L

L

G

G

T

S

L

L

V

V

S

S

Y

L

E

E

M

M

G

G

K

K

S

I

L

L

Q

V

E

E

G

G

F

V

G

G

E

E

V

V

Q

Q

E
|
E

M

Y

I

V

D

D

I

I

C

C

D

D

F

Y

A

A

V

V

G

G

L

L

S

S

R

R

Q

M

L

I

Y

G

G

G

L

P

T

I

M

L

H

P

S

S

E

E

R

R

P

S

S

G

H

H

R

A

M

L

Y

I

E

E

Q

Q

W

W

H

N

P

P

L

V

G

G

I

L

V

V

G

G

I

I

I
|
I

S
x
T

A
|
A

F
|
F

N
|
N

F
|
F

P

P

V

V

A
|
A

V
|
V

W

Y

S

G

W
|
W

N

N

T

N

A

A

L

I

A

A

L

M

V

I

C

C

G

G

N

N

V

V

C

C

I

L

W

W

K
|
K

P
x
G

S
x
A

E
x
P

K

T

T

T

P

S

L

L

T

I

A

S

I

V

A

A

C

V

Q

T

H

K

I

I

A

A

K

K

V

V

F

L

K

E

D

D

N

N

D

K

I

L

A

P

E

G

G

A

V

I

C

C

N

S

L

L

I

T

L

C

G

G

D

G

R
x
A

E

D

V

I

G
|
G

E
x
T

R

A

M

M

T

A

N

K

D

D

G

E

R

R

I

V

P

N

L

L

I

L

S

S

A
x
F

T
|
T

G
|
G

S
|
S

T

T

R

Q

M
x
V

G

G

K

K

A

Q

V
|
V

G

G

A

L

A

M

V

V

G

Q

A

E

R

R

L

F

G

G

K

R

S

S

L

L

E
|
E

L
|
L

G
|
G

G

G

N

N

A

A

I

I

I

A

S

F

E

E

H

D

A

A

D

D

L

L

D

S

M

L

S

V

L

V

I

P

G

S

A

A

V

L

F

F

G

A

A

A

V

V

G

G

T

T

A

A

G

G

Q

Q

R
|
R

C
|
C

T

T

S

T

T

A

R

R

L

L

I

F

I

I

H

H

E

E

S

S

V

I

Y

H

D

D

A

E

F

V

T

V

A

N

K

R

L

L

V

K

K

K

A

A

Y

Y

G

A

Q

Q

L

I

R

R

I

V

G

G

D

N

P

P

L

W

D

D

Q

P

N

N

N

V

H

L

V

Y

G

G

P

P

L

L

I

H

D

T

T

K

D

Q

A

A

V

V

A

S

A

M

Y

F

L

L

D

G

S

A

I

V

A

E

K

E

C

A

K

K

A

K

E

E

G

G

N

T

F

V

V

V

V

Y

E

G

G

G

G

K

V

V

L

M

S

D

G

-

D

-

A

-

Y

-

T

R

S

P

G

G

C

N

Y

Y

V

V

K

E

P

P

C

T

I

I

A

V

E

T

-

G

V

L

Q

G

N

H

D

D

F

A

K

S

I

I

V

A

Q

H

H

T

E
|
E

T

T

F
|
F

A

A

P

P

I

I

L

L

Y

Y

L

V

I

F

K

K

Y

F

K

K

T

N

L

E

D

E

E

E

A

V

I

F

A

A

L

W

Q

N

N

N

G

E

V

V

P

K

Q

Q

G

G

L

L

S

S

A

S

I

I

M

F

T

T

L

K

N

D

L

L

R

G

E

R

A

I

E

F

Q

R

F

W

L

L

S

G

A

P

K

K

G

G

S

S

D

D

C

C

G

G

I

I

A

V

N

N

V

V

N

N

I

I

G

P

T

T

S

S

G

G

A

A

E

E

I

I

G

G

A

A

F
|
F

G

G

E

E

K

K

E

H

T

T

G

G

R

R

E
|
E

S

S

G

G

S

S

D

D

A

A

W

W

R

K

A

Q

Y

Y

M

M

R

R

Q

S

T

T

N

C

T

T

I

I

N

N

Y

Y

S

S

N

K

T

D

L

L

P

P

L

L

A

A

Q

Q

G

G

I

I

K

K

F

F

D

Q

active site: N165 (= N165), K188 (= K188), E266 (= E266), C300 (= C300), E397 (= E400), E477 (= E480)
binding 4-(diethylamino)benzaldehyde: E119 (= E119), N165 (= N165), F166 (= F166), A169 (= A169), V170 (= V170), W173 (= W173), R299 (= R299), C300 (= C300), F466 (= F469)
binding nicotinamide-adenine-dinucleotide: I161 (= I161), T162 (≠ S162), A163 (= A163), F164 (= F164), N165 (= N165), K188 (= K188), G189 (≠ P189), A190 (≠ S190), P191 (≠ E191), A225 (≠ R225), G228 (= G228), T229 (≠ E229), F242 (≠ A242), T243 (= T243), G244 (= G244), S245 (= S245), V248 (≠ M248), V252 (= V252), E266 (= E266), L267 (= L267), G268 (= G268), C300 (= C300), E397 (= E400), F399 (= F402)

P49419 Alpha-aminoadipic semialdehyde dehydrogenase; Alpha-AASA dehydrogenase; Aldehyde dehydrogenase family 7 member A1; Antiquitin-1; Betaine aldehyde dehydrogenase; Delta1-piperideine-6-carboxylate dehydrogenase; P6c dehydrogenase; EC 1.2.1.31; EC 1.2.1.3; EC 1.2.1.8 from Homo sapiens (Human) (see 5 papers)
54% identity, 98% coverage: 9:512/513 of query aligns to 42:539/539 of P49419

query
sites
P49419

L

L

N

K

K

E

L

L

G

G

I

L

N

R

A

E

S

E

N

N

A

E

A

G

Y

V

S

Y

T

N

G

G

S

S

N

-

W

W

G

G

E

R

L

G

N

E

V

V

N

I

T

T

L

-

E

-

S

T

F

Y

S

C

P

P

V

A

D

N

G

N

K

E

L

P

I

I

A

A

S

R

A

V

K

R

I

Q

A

A

T

S

A

V

D

A

D

D

Y

Y

D

E

A

E

V

T

V

V

L

K

K

K

A

A

Q

R

E

E

A

A

F

W

T

K

A

I

W

W

R

A

S

D

V

I

P

P

A

A

P

P

K

K

R
|
R

G
|
G

E
|
E

I
|
I

V
|
V

R
|
R

Q
|
Q

F
x
I

G
|
G

D
|
D

A
|
A

L
|
L

R
|
R

E
|
E

N
x
K

K
x
I

D
x
Q

A
x
V

L
|
L

G
|
G

T
x
S

L
|
L

V
|
V

S
|
S

Y
x
L

E
|
E

M
|
M

G
|
G

K
|
K

S
x
I

L
|
L

Q
x
V

E
|
E

G
|
G

F
x
V

G
|
G

E
|
E

V
|
V

Q
|
Q

E
|
E

M
x
Y

I
x
V

D
|
D

I
|
I

C
|
C

D
|
D

F
x
Y

A
|
A

V
|
V

G
|
G

L
|
L

S
|
S

R
|
R

Q
x
M

L
x
I

Y
x
G

G
|
G

L
x
P

T
x
I

M
x
L

H
x
P

S
|
S

E
|
E

R
|
R

P
x
S

S
x
G

H
|
H

R
x
A

M
x
L

Y
x
I

E
|
E

Q
|
Q

W
|
W

H
x
N

P
|
P

L
x
V

G
|
G

I
x
L

V
|
V

G
|
G

I
|
I

S
x
T

A
|
A

F
|
F

N
|
N

F
|
F

P
|
P

V
|
V

A
|
A

V
|
V

W
x
Y

S
x
G

W
|
W

N
|
N

T
x
N

A
|
A

L
x
I

A
|
A

L
x
M

V
x
I

C
|
C

G
|
G

N
|
N

V
|
V

C
|
C

I
x
L

W
|
W

K
|
K

P
x
G

S
x
A

E
x
P

K
x
T

T
|
T

P
x
S

L
|
L

T
x
I

A
x
S

I
x
V

A
|
A

C
x
V

Q
x
T

H
x
K

I
|
I

A
|
A

K
|
K

V
|
V

F
x
L

K
x
E

D
|
D

N
|
N

D
x
K

I
x
L

A
x
P

E
x
G

G
x
A

V
x
I

C
|
C

N
x
S

L
|
L

I
x
T

L
x
C

G
|
G

D
x
G

R
x
A

E
x
D

V
x
I

G
|
G

E
x
T

R
x
A

M
|
M

T
x
A

N
x
K

D
|
D

G
x
E

R
|
R

I
x
V

P
x
N

L
|
L

I
x
L

S
|
S

A
x
F

T
|
T

G
|
G

S
|
S

T
|
T

R
x
Q

M
x
V

G
|
G

K
|
K

A
x
Q

V
|
V

G
|
G

A
x
L

A
x
M

V
|
V

G
x
Q

A
x
E

R
|
R

L
x
F

G
|
G

K
x
R

S
|
S

L
|
L

E
|
E

L
|
L

G
|
G

N
|
N

A
|
A

I
|
I

I
x
A

S
x
F

E
|
E

H
x
D

A
|
A

D
|
D

L
|
L

D
x
S

M
x
L

S
x
V

L
x
V

I
x
P

G
x
S

A
|
A

V
x
L

F
|
F

G
x
A

A
|
A

V
|
V

G
|
G

T
|
T

A
|
A

G
|
G

Q
|
Q

R
|
R

C
|
C

T
|
T

S
x
T

T
x
A

R
|
R

L
|
L

I
x
F

I
|
I

H
|
H

E
|
E

S
|
S

V
x
I

Y
x
H

D
|
D

A
x
E

F
x
V

T
x
V

A
x
N

K
x
R

L
|
L

V
x
K

K
|
K

A
|
A

Y
|
Y

G
x
A

Q
|
Q

L
x
I

R
|
R

I
x
V

G
|
G

D
x
N

P
|
P

L
x
W

D
|
D

Q
x
P

N
|
N

N
x
V

H
x
L

V
x
Y

G
|
G

P
|
P

L
|
L

I
x
H

D
x
T

T
x
K

D
x
Q

A
|
A

V
|
V

A
x
S

A
x
M

Y
x
F

L
|
L

D
x
G

S
x
A

I
x
V

A
x
E

K
x
E

C
x
A

K
|
K

A
x
K

E
|
E

G
|
G

N
x
T

F
x
V

V
|
V

V
x
Y

E
x
G

G
|
G

G
x
K

V
|
V

L
x
M

S
x
D

G

-

D

-

A

-

Y

-

T
x
R

S
x
P

G
|
G

C
x
N

Y
|
Y

V
|
V

K
x
E

P
|
P

C
x
T

I
|
I

A
x
V

E
x
T

-
x
G

V
x
L

Q
x
G

N
x
H

D
|
D

F
x
A

K
x
S

I
|
I

V
x
A

Q
x
H

H
x
T

E
|
E

T
|
T

F
|
F

A
|
A

P
|
P

I
|
I

L
|
L

Y
|
Y

L
x
V

I
x
F

K
|
K

Y
x
F

K
|
K

T
x
N

L
x
E

D
x
E

E
|
E

A
x
V

I
x
F

A
|
A

L
x
W

Q
x
N

N
|
N

G
x
E

V
|
V

P
x
K

Q
|
Q

G
|
G

L
|
L

S
|
S

A
x
S

I
|
I

M
x
F

T
|
T

L
x
K

N
x
D

L
|
L

R
x
G

E
x
R

A
x
I

E
x
F

Q
x
R

F
x
W

L
|
L

S
x
G

A
x
P

K
|
K

G
|
G

S
|
S

D
|
D

C
|
C

G
|
G

I
|
I

A
x
V

N
|
N

V
|
V

N
|
N

I
|
I

G
x
P

T
|
T

S
|
S

G
|
G

A
|
A

E
|
E

I
|
I

G
|
G

A
|
A

F
|
F

G
|
G

E
|
E

K
|
K

E
x
H

T
|
T

G
|
G

R
|
R

E
|
E

S
|
S

G
|
G

S
|
S

D
|
D

A
|
A

W
|
W

R
x
K

A
x
Q

Y
|
Y

M
|
M

R
|
R

Q
x
S

T
|
T

N
x
C

T
|
T

I
|
I

N
|
N

Y
|
Y

S
|
S

N
x
K

T
x
D

L
|
L

P
|
P

L
|
L

A
|
A

Q
|
Q

G
|
G

I
|
I

K
|
K

F
|
F

D
x
Q

110:539 (vs. 80:512, 57% identical) natural variant: Missing (in PDE; loss of alpha-AASA dehydrogenase activity)
TAF 192:194 (≠ SAF 162:164) binding
A199 (= A169) to V: in PDE; loss of alpha-AASA dehydrogenase activity; dbSNP:rs121912709
K218 (= K188) binding
GT 258:259 (≠ GE 228:229) binding
GS 274:275 (= GS 244:245) binding
EL 296:297 (= EL 266:267) binding
C330 (= C300) active site, Nucleophile
E427 (= E400) binding ; to Q: in PDE; loss of alpha-AASA dehydrogenase activity; dbSNP:rs121912707
K439 (= K412) to Q: in dbSNP:rs12514417

6o4dB Structure of aldh7a1 mutant w175a complexed with l-pipecolic acid (see paper)
54% identity, 98% coverage: 9:512/513 of query aligns to 13:510/510 of 6o4dB

query
sites
6o4dB

L

L

N

K

K

E

L

L

G

G

I

L

N

R

A

E

S

E

N

N

A

E

A

G

Y

V

S

Y

T

N

G

G

S

S

N

-

W

W

G

G

E

R

L

G

N

E

V

V

N

I

T

T

L

-

E

-

S

T

F

Y

S

C

P

P

V

A

D

N

G

N

K

E

L

P

I

I

A

A

S

R

A

V

K

R

I

Q

A

A

T

S

A

V

D

A

D

D

Y

Y

D

E

A

E

V

T

V

V

L

K

K

K

A

A

Q

R

E

E

A

A

F

W

T

K

A

I

W

W

R

A

S

D

V

I

P

P

A

A

P

P

K

K

R

R

G

G

E

E

I

I

V

V

R

R

Q

Q

F

I

G

G

D

D

A

A

L

L

R

R

E

E

N

K

K

I

D

Q

A

V

L

L

G

G

T

S

L

L

V

V

S

S

Y

L

E

E

M

M

G

G

K

K

S

I

L

L

Q

V

E

E

G

G

F

V

G

G

E

E

V

V

Q

Q

E
|
E

M

Y

I

V

D

D

I

I

C

C

D

D

F

Y

A

A

V

V

G

G

L

L

S

S

R

R

Q

M

L

I

Y

G

G

G

L

P

T

I

M

L

H

P

S

S

E

E

R

R

P

S

S

G

H

H

R

A

M

L

Y

I

E

E

Q

Q

W

W

H

N

P

P

L

V

G

G

I

L

V

V

G

G

I

I

S

T

A

A

F

F

N
|
N

F
|
F

P

P

V

V

A

A

V

V

W

Y

S

G

W

A

N

N

T

N

A

A

L

I

A

A

L

M

V

I

C

C

G

G

N

N

V

V

C

C

I

L

W

W

K

K

P

G

S

A

E

P

K

T

T

T

P

S

L

L

T

I

A

S

I

V

A

A

C

V

Q

T

H

K

I

I

A

A

K

K

V

V

F

L

K

E

D

D

N

N

D

K

I

L

A

P

E

G

G

A

V

I

C

C

N

S

L

L

I

T

L

C

G

G

D

G

R

A

E

D

V

I

G

G

E

T

R

A

M

M

T

A

N

K

D

D

G

E

R

R

I

V

P

N

L

L

I

L

S

S

A

F

T

T

G

G

S

S

T

T

R

Q

M

V

G

G

K

K

A

Q

V

V

G

G

A

L

A

M

V

V

G

Q

A

E

R

R

L

F

G

G

K

R

S

S

L

L

E
|
E

L

L

G

G

N

N

A

A

I

I

I

A

S

F

E

E

H

D

A

A

D

D

L

L

D

S

M

L

S

V

L

V

I

P

G

S

A

A

V

L

F

F

G

A

A

A

V

V

G

G

T

T

A

A

G

G

Q

Q

R
|
R

C
|
C

T
|
T

S

T

T

A

R

R

L

L

I

F

I

I

H

H

E

E

S

S

V

I

Y

H

D

D

A

E

F

V

T

V

A

N

K

R

L

L

V

K

K

K

A

A

Y

Y

G

A

Q

Q

L

I

R

R

I

V

G

G

D

N

P

P

L

W

D

D

Q

P

N

N

N

V

H

L

V

Y

G

G

P

P

L

L

I

H

D

T

T

K

D

Q

A

A

V

V

A

S

A

M

Y

F

L

L

D

G

S

A

I

V

A

E

K

E

C

A

K

K

A

K

E

E

G

G

N

T

F

V

V

V

V

Y

E

G

G

G

G

K

V

V

L

M

S

D

G

-

D

-

A

-

Y

-

T

R

S

P

G

G

C

N

Y

Y

V

V

K

E

P

P

C

T

I

I

A

V

E

T

-

G

V

L

Q

G

N

H

D

D

F

A

K

S

I

I

V

A

Q

H

H

T

E

E

T

T

F

F

A

A

P

P

I

I

L

L

Y

Y

L

V

I

F

K

K

Y

F

K

K

T

N

L

E

D

E

E

E

A

V

I

F

A

A

L

W

Q

N

N

N

G

E

V

V

P

K

Q

Q

G

G

L

L

S

S

A

S

I

I

M

F

T

T

L

K

N

D

L

L

R

G

E

R

A

I

E

F

Q

R

F

W

L

L

S

G

A

P

K

K

G

G

S

S

D

D

C

C

G

G

I

I

A

V

N

N

V

V

N

N

I

I

G

P

T

T

S
|
S

G
|
G

A
|
A

E

E

I

I

G

G

A

A

F
|
F

G

G

E

E

K

K

E

H

T

T

G

G

R

R

E
|
E

S

S

G

G

S

S

D

D

A

A

W

W

R

K

A

Q

Y

Y

M

M

R

R

Q

S

T

T

N

C

T

T

I

I

N

N

Y

Y

S

S

N

K

T

D

L

L

P

P

L

L

A

A

Q

Q

G

G

I

I

K

K

F

F

D

Q

active site: N166 (= N165), E267 (= E266), C301 (= C300), E478 (= E480)
binding pyridine-2-carboxylic acid: E120 (= E119), F167 (= F166), R300 (= R299), T302 (= T301), S459 (= S461), G460 (= G462), A461 (= A463), F467 (= F469)

2j6lA Structure of aminoadipate-semialdehyde dehydrogenase (see paper)
54% identity, 96% coverage: 9:500/513 of query aligns to 12:497/497 of 2j6lA

query
sites
2j6lA

L

L

N

K

K

E

L

L

G

G

I

L

N

R

A

E

S

E

N

N

A

E

A

G

Y

V

S

Y

T

N

G

G

S

S

N

-

W

W

G

G

E

R

L

G

N

E

V

V

N

I

T

T

L

-

E

-

S

T

F

Y

S

C

P

P

V

A

D

N

G

N

K

E

L

P

I

I

A

A

S

R

A

V

K

R

I

Q

A

A

T

S

A

V

D

A

D

D

Y

Y

D

E

A

E

V

T

V

V

L

K

K

K

A

A

Q

R

E

E

A

A

F

W

T

K

A

I

W

W

R

A

S

D

V

I

P

P

A

A

P

P

K

K

R

R

G

G

E

E

I

I

V

V

R

R

Q

Q

F

I

G

G

D

D

A

A

L

L

R

R

E

E

N

K

K

I

D

Q

A

V

L

L

G

G

T

S

L

L

V

V

S

S

Y

L

E

E

M

M

G

G

K

K

S

I

L

L

Q

V

E

E

G

G

F

V

G

G

E

E

V

V

Q

Q

E

E

M

Y

I

V

D

D

I

I

C

C

D

D

F

Y

A

A

V

V

G

G

L

L

S

S

R

R

Q

M

L

I

Y

G

G

G

L

P

T

I

M

L

H

P

S

S

E

E

R

R

P

S

S

G

H

H

R

A

M

L

Y

I

E

E

Q

Q

W

W

H

N

P

P

L

V

G

G

I

L

V

V

G

G

I

I

I
|
I

S
x
T

A
|
A

F
|
F

N
|
N

F

F

P

P

V

V

A

A

V

V

W

Y

S

G

W

W

N

N

T

N

A

A

L

I

A

A

L

M

V

I

C

C

G

G

N

N

V

V

C

C

I

L

W

W

K
|
K

P
x
G

S
x
A

E
x
P

K

T

T

T

P

S

L

L

T

I

A

S

I

V

A

A

C

V

Q

T

H

K

I

I

A

A

K

K

V

V

F

L

K

E

D

D

N

N

D

K

I

L

A

P

E

G

G

A

V

I

C

C

N

S

L

L

I

T

L

C

G

G

D
x
G

R
x
A

E

D

V

I

G
|
G

E
x
T

R

A

M

M

T

A

N

K

D

D

G

E

R

R

I

V

P

N

L

L

I

L

S

S

A
x
F

T
|
T

G
|
G

S
|
S

T

T

R

Q

M
x
V

G

G

K

K

A

Q

V
|
V

G

G

A

L

A

M

V

V

G

Q

A

E

R

R

L

F

G

G

K

R

S

S

L

L

E
|
E

L
|
L

G
|
G

G

G

N

N

A

A

I

I

I

A

S

F

E

E

H

D

A

A

D

D

L

L

D

S

M

L

S

V

L

V

I

P

G

S

A

A

V

L

F

F

G

A

A

A

V

V

G

G

T

T

A

A

G

G

Q

Q

R

R

C
|
C

T

T

S

T

T

A

R

R

L

L

I

F

I

I

H

H

E

E

S

S

V

I

Y

H

D

D

A

E

F

V

T

V

A

N

K

R

L

L

V

K

K

K

A

A

Y

Y

G

A

Q

Q

L

I

R

R

I

V

G

G

D

N

P

P

L

W

D

D

Q

P

N

N

N

V

H

L

V

Y

G

G

P

P

L

L

I

H

D

T

T

K

D

Q

A

A

V

V

A

S

A

M

Y

F

L

L

D

G

S

A

I

V

A

E

K

E

C

A

K

K

A

K

E

E

G

G

N

T

F

V

V

V

V

Y

E

G

G

G

G

K

V

V

L

M

S

D

G

-

D

-

A

-

Y

-

T

R

S

P

G

G

C

N

Y

Y

V

V

K

E

P

P

C

T

I

I

A

V

E

T

-

G

V

L

Q

G

N

H

D

D

F

A

K

S

I

I

V

A

Q

H

H

T

E
|
E

T

T

F
|
F

A

A

P

P

I

I

L

L

Y

Y

L

V

I

F

K

K

Y

F

K

Q

T

N

L

E

D

E

E

E

A

V

I

F

A

A

L

W

Q

N

N

N

G

E

V

V

P

K

Q

Q

G

G

L

L

S

S

A

S

I

I

M

F

T

T

L

K

N

D

L

L

R

G

E

R

A

I

E

F

Q

R

F

W

L

L

S

G

A

P

K

K

G

G

S

S

D

D

C

C

G

G

I

I

A

V

N

N

V

V

N

N

I

I

G

P

T

T

S

S

G

G

A

A

E

E

I

I

G

G

A

A

F
|
F

G

G

E

E

K

K

E

H

T

T

G

G

R

R

E
|
E

S

S

G

G

S

S

D

D

A

A

W

W

R

K

A

Q

Y

Y

M

M

R

R

Q

S

T

T

N

C

T

T

I

I

N

N

Y

Y

S

S

active site: N165 (= N165), K188 (= K188), E266 (= E266), C300 (= C300), E397 (= E400), E477 (= E480)
binding 1,4-dihydronicotinamide adenine dinucleotide: I161 (= I161), T162 (≠ S162), A163 (= A163), F164 (= F164), N165 (= N165), K188 (= K188), G189 (≠ P189), A190 (≠ S190), P191 (≠ E191), G224 (≠ D224), A225 (≠ R225), G228 (= G228), T229 (≠ E229), F242 (≠ A242), T243 (= T243), G244 (= G244), S245 (= S245), V248 (≠ M248), V252 (= V252), E266 (= E266), L267 (= L267), G268 (= G268), C300 (= C300), E397 (= E400), F399 (= F402), F466 (= F469)

6rttA Piperideine-6-carboxylate dehydrogenase from streptomyces clavuligerus complexed with picolinic acid (see paper)
55% identity, 93% coverage: 34:511/513 of query aligns to 30:507/508 of 6rttA

query
sites
6rttA

N

D

T

A

L

L

E

T

S

A

F

R

S

T

P

P

V

L

D

T

G

G

K

A

L

D

I

L

A

F

S

G

A

L

K

R

I

A

A

Q

T

T

A

P

D

E

D

D

Y

V

D

D

A

R

V

A

V

V

L

E

K

A

A

A

Q

H

E

T

A

A

F

F

T

L

A

T

W

W

R

R

S

T

V

T

P

P

A

A

P

P

K

V

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R

G

G

E

A

I

L

V

V

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F

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G

D

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A

L

L

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T

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E

N

H

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K

D

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A

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L

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L

L

V

V

S

T

Y

I

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M

A

G

G

K

K

S

I

L

R

Q

S

E

E

G

A

F

L

G

G

E

E

V

V

Q

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E
|
E

M

M

I

I

D

D

I

I

C

C

D

D

F

F

A

A

V

V

G

G

L

L

S

S

R

R

Q

Q

L

L

Y

Y

G

G

L

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T

M

M

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R

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Y

M

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W

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P

L

L

G

G

I

V

V

V

G

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I

V

I

I

S

S

A
|
A

F

F

N
|
N

F
|
F

P

P

V

V

A

A

V
|
V

W

W

S

A

W
|
W

N

N

T

A

A

A

L

V

A

A

L

L

V

V

C

C

G

G

N

D

V

T

C

V

I

V

W

W

K

K

P

P

S

S

E

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K

L

T

T

P

P

L

L

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A

A

I

L

A

A

C

C

Q

A

H

A

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L

A

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K

L

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A

F

I

K

A

D

D

N

A

D

G

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A

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N

N

Q

L

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V

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D

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A

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G
|
G

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|
S

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M

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A

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A

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A

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|
R

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x
F

G

G

K

R

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T

L

I

L

L

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|
E

L

L

G

G

N

N

N
&nb