SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing CA265_RS21455 FitnessBrowser__Pedo557:CA265_RS21455 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 15 hits to proteins with known functional sites (download)

3pzlB The crystal structure of agmatine ureohydrolase of thermoplasma volcanium
24% identity, 83% coverage: 52:320/326 of query aligns to 23:283/293 of 3pzlB

query
sites
3pzlB

A

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active site: H112 (= H150), D132 (= D174), H134 (= H176), D136 (= D178), H148 (≠ Y193), D217 (= D257), D219 (= D259), E260 (= E297)
binding manganese (ii) ion: H112 (= H150), D132 (= D174), D132 (= D174), H134 (= H176), D136 (= D178), D217 (= D257), D217 (= D257), D219 (= D259)

4mynA Crystal structure of trypanosoma cruzi formiminoglutamase n114h variant with mn2+2 (see paper)
29% identity, 46% coverage: 48:198/326 of query aligns to 14:161/298 of 4mynA

query
sites
4mynA

K

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active site: H110 (= H150), D134 (= D174), H136 (= H176), D138 (= D178), S150 (= S187)
binding manganese (ii) ion: H110 (= H150), D134 (= D174), D134 (= D174), H136 (= H176), D138 (= D178)

Sites not aligning to the query:

active site: 224, 226, 269
binding manganese (ii) ion: 224, 224, 226

5cevA Arginase from bacillus caldevelox, l-lysine complex (see paper)
25% identity, 41% coverage: 56:189/326 of query aligns to 5:136/298 of 5cevA

query
sites
5cevA

L

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active site: H98 (= H150), D121 (= D174), H123 (= H176), D125 (= D178)
binding lysine: S134 (= S187)
binding manganese (ii) ion: H98 (= H150), D121 (= D174), D121 (= D174), H123 (= H176), D125 (= D178)

Sites not aligning to the query:

active site: 138, 225, 227, 270
binding guanidine: 251, 255
binding lysine: 138, 270
binding manganese (ii) ion: 225, 225, 227

4cevA Arginase from bacillus caldevelox, l-ornithine complex (see paper)
25% identity, 41% coverage: 56:189/326 of query aligns to 5:136/298 of 4cevA

query
sites
4cevA

L

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active site: H98 (= H150), D121 (= D174), H123 (= H176), D125 (= D178)
binding manganese (ii) ion: H98 (= H150), D121 (= D174), D121 (= D174), H123 (= H176), D125 (= D178)
binding L-ornithine: H123 (= H176), D125 (= D178), S134 (= S187)

Sites not aligning to the query:

active site: 138, 225, 227, 270
binding guanidine: 251, 255
binding manganese (ii) ion: 225, 225, 227
binding L-ornithine: 138, 177

3cevA Arginase from bacillus caldevelox, complexed with l-arginine (see paper)
25% identity, 41% coverage: 56:189/326 of query aligns to 5:136/298 of 3cevA

query
sites
3cevA

L

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active site: H98 (= H150), D121 (= D174), H123 (= H176), D125 (= D178)
binding arginine: H123 (= H176), D125 (= D178), S134 (= S187)
binding manganese (ii) ion: H98 (= H150), D121 (= D174), D125 (= D178)

Sites not aligning to the query:

active site: 138, 225, 227, 270
binding arginine: 138, 225, 251, 255, 270, 295, 297
binding manganese (ii) ion: 225

2cevB Arginase from bacillus caldevelox, native structure at ph 8.5 (see paper)
25% identity, 41% coverage: 56:189/326 of query aligns to 5:136/298 of 2cevB

query
sites
2cevB

L

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G

N

N

active site: H98 (= H150), D121 (= D174), H123 (= H176), D125 (= D178)
binding manganese (ii) ion: H98 (= H150), D121 (= D174), D121 (= D174), H123 (= H176), D125 (= D178)

Sites not aligning to the query:

active site: 138, 225, 227, 270
binding guanidine: 251, 255
binding manganese (ii) ion: 225, 225, 227

1cevA Arginase from bacillus caldovelox, native structure at ph 5.6 (see paper)
25% identity, 41% coverage: 56:189/326 of query aligns to 6:137/299 of 1cevA

query
sites
1cevA

L

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L

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D

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N

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S

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G

N

N

active site: H99 (= H150), D122 (= D174), H124 (= H176), D126 (= D178)
binding manganese (ii) ion: H99 (= H150), D122 (= D174), D122 (= D174), H124 (= H176), D126 (= D178)

Sites not aligning to the query:

active site: 139, 226, 228, 271
binding manganese (ii) ion: 226, 226, 228

P53608 Arginase; EC 3.5.3.1 from Bacillus caldovelox (see paper)
25% identity, 41% coverage: 56:189/326 of query aligns to 6:137/299 of P53608

query
sites
P53608

L

I

L

I

G

G

I

V

P

P

E

M

D

D

I

L

G

G

V

Q

R

T

A

R

N

R

L

G

G

V

L

D

A

M

G

G

A

P

A

S

S

A

M

M

W

R

K

Y

P

A

G

G

L

V

V

I

A

E

F

R

L

L

N

E

T

R

Q

L

S

H

N

Y

R

D

F

I

L

E

S

D

G

L

E

G

E

D

V

I

L

P

V

I

L

G

G

K

H

A

F

E

E

R

I

L

D

H

E

E

P

Q

E

G

D

D

S

S

S

R

L

L

K

R

G

N

L

L

R

K

N

-

K

A

V

V

A

A

Q

E

I

A

D

N

D

E

L

K

V

L

Y

A

P

A

V

A

I

V

E

D

K

Q

I

V

V

V

A

Q

T

R

G

G

K

R

I

F

P

P

V

L

V

V

I

L

G

G

G

G

G

D

H
|
H

N

S

N

I

A

A

Y

I

P

G

M

T

I

L

K

A

G

G

T

V

S

A

L

K

A

H

H

Y

K

E

R

R

P

-

I

L

T

G

V

V

L

I

N

W

V

Y

D
|
D

A

A

H
|
H

A
x
G

D
|
D

L
x
V

R
x
N

E

T

L

A

E

E

G

T

R

S

H

P

S
|
S

G
|
G

N
|
N

H99 (= H150) binding
D122 (= D174) binding ; binding
H124 (= H176) binding
HGDVN 124:128 (≠ HADLR 176:180) binding
D126 (= D178) binding
SGN 135:137 (= SGN 187:189) binding

Sites not aligning to the query:

178 binding
226 binding ; binding
228 binding
240 binding
271 binding

6nbkA Crystal structure of arginase from bacillus cereus
26% identity, 50% coverage: 56:218/326 of query aligns to 5:161/289 of 6nbkA

query
sites
6nbkA

L

V

L

I

G

G

I

V

P

P

E

M

D

D

I

L

G

G

-

Q

V

M

R

R

A

R

N

G

L

V

G

D

L

M

A

-

G

G

A

P

A

S

S

A

M

I

W

R

K

Y

P

A

G

G

L

V

V

I

A

E

F

R

L

I

N

E

T

E

Q

-

S

-

N

-

R

-

F

-

L

-

S

I

G

G

E

Y

E

D

V

V

L

K

V

D

L

M

G

G

H

D

F

I

E

C

I

I

D

E

E

E

P

-

E

-

D

N

S

T

S

K

L

L

K

R

G

N

L

L

R

-

N

T

K

Q

V

V

A

A

Q

T

I

V

D

C

D

N

L

E

V

L

Y

A

P

S

V

K

I

V

E
x
D

K

H

I

I

V

I

A
x
E

T

E

G

G

K

R

I

F

P

P

V

L

V

V

I

L

G

G

G

G

G

D

H
|
H

N

S

N

I

A

A

Y

I

P

G

M

T

I

L

K

A

G

G

T

V

S

A

L

-

A

K

H

H

K

Y

R

K

P

N

I

L

T

G

V

V

L

I

N

W

V

Y

D
|
D

A

A

H
|
H

A

G

D
|
D

L

L

R

N

E

T

L

E

E

E

G

T

R

S

H

P

S

S

G

G

N

N

-

I

-

H

G

G

F

M

S

S

Y

L

A

A

L

A

K

S

E

L

N

G

Y

Y

L

G

N

H

N

S

Y

S

L

L

-

V

-

D

M

L

Y

Y

G

G

L

A

H

Y

Q

P

N

K

Y

V

N

K

N

K

E

E

A

N

I

V

L

V

binding manganese (ii) ion: D74 (≠ E134), E78 (≠ A138), H90 (= H150), D113 (= D174), D113 (= D174), H115 (= H176), D117 (= D178)

Sites not aligning to the query:

binding manganese (ii) ion: 216, 216, 218

5hjaA Crystal structure of leishmania mexicana arginase in complex with inhibitor abhdp (see paper)
33% identity, 21% coverage: 123:189/326 of query aligns to 75:140/303 of 5hjaA

query
sites
5hjaA

A

A

Q

E

I

C

D

T

D

E

L

K

V

I

Y

Y

P

K

V

C

I

V

E

R

K

R

I

V

V

A

A

E

T

Q

G

G

K

R

I

F

P

P

V

L

V

T

I

I

G

G

G

G

G

D

H
|
H

N

S

N

I

A

A

Y

L

P

G

M

T

I

V

K

A

G

G

T

V

S

L

L

S

A

V

H

H

K

P

R

D

P

-

I

A

T

G

V

V

L

I

N

W

V

V

D
|
D

A

A

H
|
H

A

A

D
|
D

L

I

R

N

E

T

L

M

E

S

G

G

R

T

H

V

S
|
S

G

G

N

N

active site: H102 (= H150), D125 (= D174), H127 (= H176), D129 (= D178)
binding manganese (ii) ion: H102 (= H150), D125 (= D174), D125 (= D174), H127 (= H176), D129 (= D178)
binding (R)-2-amino-6-borono-2-(1-(3,4-dichlorobenzyl)piperidin-4-yl)hexanoic acid: H102 (= H150), D125 (= D174), H127 (= H176), D129 (= D178), S138 (= S187)

Sites not aligning to the query:

active site: 142, 231, 233, 276
binding manganese (ii) ion: 231, 231, 233
binding (R)-2-amino-6-borono-2-(1-(3,4-dichlorobenzyl)piperidin-4-yl)hexanoic acid: 142, 143, 180, 181, 182, 231, 233

4iu5A Crystal structure of leishmania mexicana arginase in complex with catalytic product l-ornithine (see paper)
33% identity, 21% coverage: 123:189/326 of query aligns to 75:140/310 of 4iu5A

query
sites
4iu5A

A

A

Q

E

I

C

D

T

D

E

L

K

V

I

Y

Y

P

K

V

C

I

V

E

R

K

R

I

V

V

A

A

E

T

Q

G

G

K

R

I

F

P

P

V

L

V

T

I

I

G

G

G

G

G

D

H
|
H

N

S

N

I

A

A

Y

L

P

G

M

T

I

V

K

A

G

G

T

V

S

L

L

S

A

V

H

H

K

P

R

D

P

-

I

A

T

G

V

V

L

I

N

W

V

V

D
|
D

A

A

H
|
H

A

A

D
|
D

L

I

R

N

E

T

L

M

E

S

G

G

R

T

H

V

S
|
S

G

G

N

N

active site: H102 (= H150), D125 (= D174), H127 (= H176), D129 (= D178)
binding manganese (ii) ion: H102 (= H150), D125 (= D174), D125 (= D174), H127 (= H176), D129 (= D178)
binding L-ornithine: H127 (= H176), D129 (= D178), S138 (= S187)

Sites not aligning to the query:

active site: 142, 231, 233, 276
binding manganese (ii) ion: 231, 231, 233
binding L-ornithine: 142

4iu4A Crystal structure of leishmania mexicana arginase in complex with inhibitor bec (see paper)
33% identity, 21% coverage: 123:189/326 of query aligns to 75:140/310 of 4iu4A

query
sites
4iu4A

A

A

Q

E

I

C

D

T

D

E

L

K

V

I

Y

Y

P

K

V

C

I

V

E

R

K

R

I

V

V

A

A

E

T

Q

G

G

K

R

I

F

P

P

V

L

V

T

I

I

G

G

G

G

G

D

H
|
H

N

S

N

I

A

A

Y

L

P

G

M

T

I

V

K

A

G

G

T

V

S

L

L

S

A

V

H

H

K

P

R

D

P

-

I

A

T

G

V

V

L

I

N

W

V

V

D
|
D

A

A

H
|
H

A

A

D
|
D

L

I

R

N

E

T

L

M

E

S

G

G

R

T

H

V

S
|
S

G

G

N

N

active site: H102 (= H150), D125 (= D174), H127 (= H176), D129 (= D178)
binding manganese (ii) ion: H102 (= H150), D125 (= D174), D125 (= D174), H127 (= H176), D129 (= D178)
binding s-2-(boronoethyl)-l-cysteine: D125 (= D174), H127 (= H176), D129 (= D178), S138 (= S187)

Sites not aligning to the query:

active site: 142, 231, 233, 276
binding manganese (ii) ion: 231, 231, 233
binding s-2-(boronoethyl)-l-cysteine: 142, 182, 231, 233, 276

4iu1A Crystal structure of leishmania mexicana arginase in complex with inhibitor nor-noha (see paper)
33% identity, 21% coverage: 123:189/326 of query aligns to 75:140/310 of 4iu1A

query
sites
4iu1A

A

A

Q

E

I

C

D

T

D

E

L

K

V

I

Y

Y

P

K

V

C

I

V

E

R

K

R

I

V

V

A

A

E

T

Q

G

G

K

R

I

F

P

P

V

L

V

T

I

I

G

G

G

G

G

D

H
|
H

N

S

N

I

A

A

Y

L

P

G

M

T

I

V

K

A

G

G

T

V

S

L

L

S

A

V

H

H

K

P

R

D

P

-

I

A

T

G

V

V

L

I

N

W

V

V

D
|
D

A

A

H
|
H

A

A

D
|
D

L

I

R
x
N

E

T

L

M

E

S

G

G

R

T

H

V

S
|
S

G

G

N

N

active site: H102 (= H150), D125 (= D174), H127 (= H176), D129 (= D178)
binding manganese (ii) ion: H102 (= H150), D125 (= D174), D125 (= D174), H127 (= H176), D129 (= D178)
binding nor-n-omega-hydroxy-l-arginine: H127 (= H176), D129 (= D178), N131 (≠ R180), S138 (= S187)

Sites not aligning to the query:

active site: 142, 231, 233, 276
binding manganese (ii) ion: 231, 231, 233
binding nor-n-omega-hydroxy-l-arginine: 142, 182, 245

4iu0A Crystal structure of leishmania mexicana arginase in complex with inhibitor abh (see paper)
33% identity, 21% coverage: 123:189/326 of query aligns to 75:140/310 of 4iu0A

query
sites
4iu0A

A

A

Q

E

I

C

D

T

D

E

L

K

V

I

Y

Y

P

K

V

C

I

V

E

R

K

R

I

V

V

A

A

E

T

Q

G

G

K

R

I

F

P

P

V

L

V

T

I

I

G

G

G

G

G

D

H
|
H

N

S

N

I

A

A

Y

L

P

G

M

T

I

V

K

A

G

G

T

V

S

L

L

S

A

V

H

H

K

P

R

D

P

-

I

A

T

G

V

V

L

I

N

W

V

V

D
|
D

A

A

H
|
H

A

A

D
|
D

L

I

R

N

E

T

L

M

E

S

G

G

R

T

H

V

S
|
S

G

G

N

N

active site: H102 (= H150), D125 (= D174), H127 (= H176), D129 (= D178)
binding 2(s)-amino-6-boronohexanoic acid: D125 (= D174), H127 (= H176), D129 (= D178), S138 (= S187)
binding manganese (ii) ion: H102 (= H150), D125 (= D174), D125 (= D174), H127 (= H176), D129 (= D178)

Sites not aligning to the query:

active site: 142, 231, 233, 276
binding 2(s)-amino-6-boronohexanoic acid: 142, 231, 276
binding manganese (ii) ion: 231, 231, 233

6nfpA 1.7 angstrom resolution crystal structure of arginase from bacillus subtilis subsp. Subtilis str. 168
32% identity, 39% coverage: 63:189/326 of query aligns to 6:131/292 of 6nfpA

query
sites
6nfpA

I

I

G

G

V

M

R

P

A

M

N

D

L

L

G

G

L

Q

A

A

G

R

A

R

A

G

S

V

M

D

W

M

K

G

P

P

G

S

L

A

V

I

A

R

F

Y

L

A

N

H

T

L

Q

I

S

E

N

R

R

L

F

S

L

D

S

M

G

G

E

Y

E

T

V

V

L

E

V

D

L

L

G

G

H

D

F

I

E

P

I

I

D

N

-

R

E

E

P

K

E

I

D

D

S

E

S

E

L

L

K

K

G

N

L

L

R

N

N

S

K

V

V

L

A

A

Q

G

I

N

D

E

D

K

L

L

V

A

Y

Q

P

K

V

V

I

-

E

N

K

K

I

V

V

I

A

E

T

E

G

K

K

K

I

F

P

P

V

L

V

V

I

L

G

G

G

G

G

D

H
|
H

N

S

N

I

A

A

Y

I

P

G

M

T

I

L

K

A

G

G

T

T

S

A

L

-

A

K

H

H

K

Y

R

D

P

N

I

L

T

G

V

V

L

I

N

W

V

Y

D
|
D

A

A

H
|
H

A

G

D
|
D

L

L

R

N

E

T

L

L

E

E

G

T

R

S

H

P

S

S

G

G

N

N

binding magnesium ion: H93 (= H150), D116 (= D174), D116 (= D174), H118 (= H176), D120 (= D178)

Sites not aligning to the query:

binding magnesium ion: 219, 219, 221

Query Sequence

>CA265_RS21455 FitnessBrowser__Pedo557:CA265_RS21455
MDNLKIYSQGDIDHLIITRDGETKLGERVNVYSGDSSSTAGISVEGLKASSAKFVLLGIP
EDIGVRANLGLAGAASMWKPGLVAFLNTQSNRFLSGEEVLVLGHFEIDEPEDSSLKGLRN
KVAQIDDLVYPVIEKIVATGKIPVVIGGGHNNAYPMIKGTSLAHKRPITVLNVDAHADLR
ELEGRHSGNGFSYALKENYLNNYLMYGLHQNYNNEAILNQIDTNPKLKAVFFDDILTGAD
FTNLVNEIGSVAGLEIDLDCVQNVLSSAETPSGFAVNDIRKLILTSAKKFSYLHLSEGAT
RMLDGRVSKLTSKLVAYLVSDFIKAH

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory