SitesBLAST
Comparing CA265_RS23175 FitnessBrowser__Pedo557:CA265_RS23175 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
5aovA Ternary crystal structure of pyrococcus furiosus glyoxylate hydroxypyruvate reductase in presence of glyoxylate (see paper)
36% identity, 88% coverage: 31:303/309 of query aligns to 34:308/334 of 5aovA
- active site: L100 (≠ N97), R241 (= R229), D265 (= D253), E270 (≠ Q266), H288 (= H283)
- binding glyoxylic acid: M52 (≠ R49), L53 (≠ T50), L53 (≠ T50), Y74 (≠ A71), A75 (≠ G72), V76 (≠ A73), G77 (= G74), R241 (= R229), H288 (= H283)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V76 (≠ A73), T104 (≠ V101), F158 (≠ Y148), G159 (= G149), R160 (≠ F150), I161 (≠ M151), S180 (≠ D170), R181 (≠ K171), A211 (≠ H199), V212 (≠ I200), P213 (= P201), T218 (= T206), I239 (≠ T227), A240 (= A228), R241 (= R229), H288 (= H283), G290 (= G285)
1wwkA Crystal structure of phosphoglycerate dehydrogenase from pyrococcus horikoshii ot3
36% identity, 97% coverage: 3:303/309 of query aligns to 2:298/304 of 1wwkA
- active site: S96 (≠ N97), R230 (= R229), D254 (= D253), E259 (= E258), H278 (= H283)
- binding nicotinamide-adenine-dinucleotide: V100 (= V101), G146 (= G147), F147 (≠ Y148), G148 (= G149), R149 (≠ F150), I150 (≠ M151), Y168 (= Y169), D169 (= D170), P170 (≠ K171), V201 (≠ I200), P202 (= P201), T207 (= T206), T228 (= T227), S229 (≠ A228), D254 (= D253), H278 (= H283), G280 (= G285)
7dkmA Phgdh covalently linked to oridonin (see paper)
31% identity, 97% coverage: 1:300/309 of query aligns to 2:296/306 of 7dkmA
- binding nicotinamide-adenine-dinucleotide: T74 (≠ A73), A102 (≠ V101), G148 (= G147), R151 (≠ F150), I152 (≠ M151), Y170 (= Y169), D171 (= D170), P172 (≠ K171), I173 (≠ Y172), H202 (= H199), T203 (≠ I200), P204 (= P201), T209 (= T206), C230 (≠ T227), A231 (= A228), R232 (= R229), H279 (= H283), G281 (= G285)
- binding (1beta,6beta,7beta,8alpha,9beta,10alpha,13alpha,14R,16beta)-1,6,7,14-tetrahydroxy-7,20-epoxykauran-15-one: C14 (≠ A13), K17 (≠ E16), I18 (≠ Q17), E293 (= E297)
6plgA Crystal structure of human phgdh complexed with compound 15 (see paper)
31% identity, 97% coverage: 1:300/309 of query aligns to 1:295/303 of 6plgA
6rj5A Crystal structure of phgdh in complex with compound 39 (see paper)
31% identity, 97% coverage: 1:300/309 of query aligns to 1:295/301 of 6rj5A
7ewhA Crystal structure of human phgdh in complex with homoharringtonine (see paper)
31% identity, 97% coverage: 1:300/309 of query aligns to 1:295/302 of 7ewhA
- binding (3beta)-O~3~-[(2R)-2,6-dihydroxy-2-(2-methoxy-2-oxoethyl)-6-methylheptanoyl]cephalotaxine: L146 (≠ I146), G147 (= G147), L148 (≠ Y148), G149 (= G149), R150 (≠ F150), I151 (≠ M151), G152 (= G152), D170 (= D170), H201 (= H199), T202 (≠ I200), P203 (= P201)
6rihA Crystal structure of phgdh in complex with compound 9 (see paper)
31% identity, 97% coverage: 1:300/309 of query aligns to 1:295/302 of 6rihA
6plfA Crystal structure of human phgdh complexed with compound 1 (see paper)
31% identity, 97% coverage: 1:300/309 of query aligns to 2:296/305 of 6plfA
6rj3A Crystal structure of phgdh in complex with compound 15 (see paper)
31% identity, 96% coverage: 3:300/309 of query aligns to 2:294/297 of 6rj3A
O43175 D-3-phosphoglycerate dehydrogenase; 3-PGDH; 2-oxoglutarate reductase; Malate dehydrogenase; EC 1.1.1.95; EC 1.1.1.399; EC 1.1.1.37 from Homo sapiens (Human) (see 3 papers)
31% identity, 97% coverage: 1:300/309 of query aligns to 6:300/533 of O43175
- T78 (≠ A73) binding
- R135 (= R130) to W: in PHGDHD; results in a 2-fold decrease in enzyme activity with 3-phosphohydroxypyruvate, but no change in substrate affinity; dbSNP:rs267606949
- RI 155:156 (≠ FM 150:151) binding
- D175 (= D170) binding
- T207 (≠ I200) binding
- CAR 234:236 (≠ TAR 227:229) binding
- D260 (= D253) binding
- V261 (= V254) to M: in PHGDHD; results in a four-fold decrease in substrate affinity and a slight increase in maximal enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs267606947
- HLGA 283:286 (≠ HVGG 283:286) binding
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 2 modified: N-acetylalanine
- 373 A → T: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs201553627
- 377 G → S: in PHGDHD; results in a 2-fold decrease in enzyme activity with 3-phosphohydroxypyruvate, but no change in substrate affinity; dbSNP:rs267606948
- 425 V → M: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs121907988
- 490 V → M: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs121907987
6cwaA Crystal structure phgdh in complex with nadh and 3-phosphoglycerate at 1.77 a resolution (see paper)
31% identity, 96% coverage: 3:300/309 of query aligns to 2:294/299 of 6cwaA
- binding 1,4-dihydronicotinamide adenine dinucleotide: N96 (= N97), A100 (≠ V101), R149 (≠ F150), I150 (≠ M151), Y168 (= Y169), D169 (= D170), P170 (≠ K171), I171 (≠ Y172), H200 (= H199), T201 (≠ I200), P202 (= P201), T207 (= T206), C228 (≠ T227), A229 (= A228), R230 (= R229), H277 (= H283), G279 (= G285)
6rj2A Crystal structure of phgdh in complex with compound 40 (see paper)
31% identity, 96% coverage: 4:300/309 of query aligns to 1:292/299 of 6rj2A
- binding ~{N}-[(1~{R})-1-[4-(ethanoylsulfamoyl)phenyl]ethyl]-2-methyl-5-phenyl-pyrazole-3-carboxamide: G146 (= G149), I148 (≠ M151), Y166 (= Y169), D167 (= D170), P168 (≠ K171), I169 (≠ Y172), I170 (≠ K173), H198 (= H199), T199 (≠ I200), L208 (≠ M209), R228 (= R229)
3ddnB Crystal structure of hydroxypyruvic acid phosphate bound d-3- phosphoglycerate dehydrogenase in mycobacterium tuberculosis (see paper)
35% identity, 97% coverage: 4:303/309 of query aligns to 5:298/525 of 3ddnB
3dc2A Crystal structure of serine bound d-3-phosphoglycerate dehydrogenase from mycobacterium tuberculosis (see paper)
35% identity, 97% coverage: 4:303/309 of query aligns to 4:297/526 of 3dc2A
Sites not aligning to the query:
2eklA Structure of st1218 protein from sulfolobus tokodaii
31% identity, 97% coverage: 3:303/309 of query aligns to 6:302/312 of 2eklA
- active site: S100 (≠ N97), R232 (= R229), D256 (= D253), E261 (= E258), H282 (= H283)
- binding nicotinamide-adenine-dinucleotide: I76 (≠ A73), S100 (≠ N97), G148 (= G147), G150 (= G149), R151 (≠ F150), I152 (≠ M151), Y170 (= Y169), D171 (= D170), I172 (vs. gap), L173 (vs. gap), H202 (= H199), V203 (≠ I200), T204 (≠ P201), I212 (≠ M209), T230 (= T227), S231 (≠ A228), D256 (= D253), G284 (= G285)
P87228 Putative D-3-phosphoglycerate dehydrogenase; 3-PGDH; 2-oxoglutarate reductase; EC 1.1.1.95; EC 1.1.1.399 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
30% identity, 98% coverage: 3:304/309 of query aligns to 57:365/466 of P87228
- S87 (≠ T32) modified: Phosphoserine
- S258 (≠ T203) modified: Phosphoserine
6biiA Crystal structure of pyrococcus yayanosii glyoxylate hydroxypyruvate reductase in complex with NADP and malonate (re-refinement of 5aow) (see paper)
35% identity, 88% coverage: 31:303/309 of query aligns to 33:307/332 of 6biiA
- active site: L99 (≠ N97), R240 (= R229), D264 (= D253), E269 (= E258), H287 (= H283)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V75 (≠ A73), T103 (≠ V101), G156 (= G147), F157 (≠ Y148), G158 (= G149), R159 (≠ F150), I160 (≠ M151), A179 (= A168), R180 (≠ Y169), S181 (≠ D170), K183 (≠ Y172), V211 (≠ I200), P212 (= P201), E216 (= E205), T217 (= T206), V238 (≠ T227), A239 (= A228), R240 (= R229), D264 (= D253), H287 (= H283), G289 (= G285)
6plfB Crystal structure of human phgdh complexed with compound 1 (see paper)
32% identity, 96% coverage: 3:300/309 of query aligns to 2:286/292 of 6plfB
- binding 4-{(1S)-1-[(5-chloro-6-{[(5S)-2-oxo-1,3-oxazolidin-5-yl]methoxy}-1H-indole-2-carbonyl)amino]-2-hydroxyethyl}benzoic acid: R141 (≠ F150), Y160 (= Y169), D161 (= D170), P162 (≠ K171), I164 (≠ K173), L179 (≠ M186), T193 (≠ I200), P194 (= P201), S198 (≠ E205), L202 (≠ M209)
O58320 Glyoxylate reductase; EC 1.1.1.26 from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
35% identity, 90% coverage: 27:303/309 of query aligns to 30:308/334 of O58320
2dbqA Crystal structure of glyoxylate reductase (ph0597) from pyrococcus horikoshii ot3, complexed with NADP (i41) (see paper)
35% identity, 90% coverage: 27:303/309 of query aligns to 30:308/333 of 2dbqA
- active site: L100 (≠ N97), R241 (= R229), D265 (= D253), E270 (≠ Q266), H288 (= H283)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V76 (≠ A73), T104 (≠ V101), L158 (≠ Y148), G159 (= G149), R160 (≠ F150), I161 (≠ M151), S180 (≠ D170), R181 (≠ K171), T182 (≠ Y172), A211 (≠ H199), V212 (≠ I200), P213 (= P201), T218 (= T206), I239 (≠ T227), A240 (= A228), R241 (= R229), D265 (= D253), H288 (= H283), G290 (= G285)
Query Sequence
>CA265_RS23175 FitnessBrowser__Pedo557:CA265_RS23175
MNKILIVDDLHPAFKEQAIAMGYEVDDEPQITRQQTLDKIKDYTGIAVRTKFRIDAEIFA
AAPNLKFVARAGAGLDNIDDKIAFERNIELINAPEGNCDAVGEHATGLLLSLMNNFRRAD
IEIRNGVWDREGNRGYELKGKKVGIIGYGFMGQSFAKKLAGFEVDVMAYDKYKTGFSDAF
AREVSMEEIVKHSDVLSLHIPLTAETKQMVDDEYFYHFKKPIFFINTARGEIVNTPAVLN
AIKSGKILGAGLDVLQTEKFPALGEQAWYNELKNNDKVILTPHVGGWTFDSYRKISEVLA
EKLSGLSFD
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory