SitesBLAST

P24232 Flavohemoprotein; Flavohemoglobin; HMP; Hemoglobin-like protein; Nitric oxide dioxygenase; NO oxygenase; NOD; EC 1.14.12.17 from Escherichia coli (strain K12) (see 2 papers)
27% identity, 64% coverage: 18:238/348 of query aligns to 169:390/396 of P24232

query
sites
P24232

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Sites not aligning to the query:

29 mutation Y->E,H: 15 to 35-fold reduction in NO dioxygenase activity.; Y→F: 30-fold reduction in NO dioxygenase activity, and 80-fold increase in the O(2) dissociation rate constant.
95 active site, Charge relay system
135 active site, Charge relay system

4eh1A Crystal structure of the flavohem-like-fad/NAD binding domain of nitric oxide dioxygenase from vibrio cholerae o1 biovar el tor
31% identity, 60% coverage: 31:238/348 of query aligns to 34:235/237 of 4eh1A

query
sites
4eh1A

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binding flavin-adenine dinucleotide: Y39 (≠ F36), R55 (= R50), Q56 (≠ S51), Y57 (= Y52), S58 (= S53), S71 (≠ T67), V72 (= V68), E75 (≠ V71), N81 (vs. gap), G83 (vs. gap), L84 (vs. gap), V85 (≠ I76), S86 (= S77), T127 (= T121), E233 (= E236), F235 (= F238)

3b2gA Leptolyngbya boryana ferredoxin (see paper)
44% identity, 21% coverage: 274:346/348 of query aligns to 18:90/98 of 3b2gA

query
sites
3b2gA

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binding fe2/s2 (inorganic) cluster: S40 (= S296), C41 (= C297), R42 (= R298), G44 (= G300), C46 (= C302), C49 (= C305), C79 (= C335)

1cnfA Structural studies on corn nitrate reductase: refined structure of the cytochrome b reductase fragment at 2.5 angstroms, its adp complex and an active site mutant and modeling of the cytochrome b domain (see paper)
35% identity, 30% coverage: 109:212/348 of query aligns to 127:234/260 of 1cnfA

query
sites
1cnfA

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active site: C232 (= C210)
binding adenosine-5'-diphosphate: N165 (= N145), P234 (= P212)
binding flavin-adenine dinucleotide: S136 (≠ V118), T139 (= T121), P140 (= P122)

Sites not aligning to the query:

active site: 38, 54, 55
binding adenosine-5'-diphosphate: 71, 236, 237, 240, 241
binding flavin-adenine dinucleotide: 52, 53, 54, 69, 73, 81, 84, 85, 86, 87, 88

1rfkB Crystal structure of 2fe2s ferredoxin from thermophilic cyanobacterium mastigocladus laminosus (see paper)
42% identity, 24% coverage: 260:342/348 of query aligns to 2:86/97 of 1rfkB

query
sites
1rfkB

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binding fe2/s2 (inorganic) cluster: Y39 (= Y295), C41 (= C297), R42 (= R298), G44 (= G300), C46 (= C302), C49 (= C305), C79 (= C335)

7s3dX Structure of photosystem i with bound ferredoxin from synechococcus sp. Pcc 7335 acclimated to far-red light (see paper)
48% identity, 19% coverage: 282:346/348 of query aligns to 25:89/97 of 7s3dX

query
sites
7s3dX

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binding fe2/s2 (inorganic) cluster: Y38 (= Y295), S39 (= S296), C40 (= C297), R41 (= R298), G43 (= G300), C45 (= C302), C48 (= C305), C78 (= C335)

P17571 Nitrate reductase [NADH] 1; NR; EC 1.7.1.1 from Zea mays (Maize) (see 3 papers)
35% identity, 29% coverage: 111:212/348 of query aligns to 490:595/621 of P17571

query
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P17571

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S497 (≠ V118) binding
T500 (= T121) binding
C593 (= C210) Necessary for efficient electron Transfer; mutation to S: Reduction of activity.

Sites not aligning to the query:

413:416 binding
430:432 binding
435 binding
447:449 binding

7fixR1 Photosystem I reaction center subunit PsaK (see paper)
43% identity, 23% coverage: 260:339/348 of query aligns to 2:82/97 of 7fixR1

query
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7fixR1

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binding [2Ga-2S] cluster: F38 (≠ Y295), S39 (= S296), C40 (= C297), R41 (= R298), G43 (= G300), C45 (= C302), C48 (= C305), L76 (= L333), C78 (= C335)

6khi1 Supercomplex for cylic electron transport in cyanobacteria (see paper)
43% identity, 23% coverage: 260:339/348 of query aligns to 3:83/98 of 6khi1

query
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6khi1

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binding fe2/s2 (inorganic) cluster: C41 (= C297), R42 (= R298), G44 (= G300), C46 (= C302), C49 (= C305), L77 (= L333), C79 (= C335)

Q9ZNT1 NADH--cytochrome b5 reductase 1; EC 1.6.2.2 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
28% identity, 43% coverage: 64:212/348 of query aligns to 112:257/281 of Q9ZNT1

query
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Q9ZNT1

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-

E

-

R

R

D

A

L

F

F

G

L

M

F

L

A

A

A

G

G

G

V

S

G

G

I

I

T

T

P

P

L

M

F

F

S

Q

I

V

L

A

K

R

T

A

A

I

L

L

V

E

R

N

E

P

K

T

-

D

K

K

S

T

L

K

V

V

T

H

L

L

V

I

Y

Y

S

A

N

N

R

V

S

T

M

Y

E

D

D

D

A

I

L

L

F

L

Y

K

D

E

E

E

L

L

S

E

E

G

W

L

Q

T

K

T

K

N

Y

Y

P

P

E

E

R

Q

L

F

K

K

I

I

V

F

W

Y

V

V

F

L

S

N

N

Q

S

P

K

P

N

E

L

V

M

W

T

D

A

G

R

G

L

V

N

G

K

-

F

F

Y

V

I

S

E

K

K

E

L

M

L

I

K

Q

E

T

H

H

L

C

H

P

F

A

D

P

R

A

N

S

D

D

A

I

L

Q

F

I

Y

L

S

R

C

C

G

G

P

P

Sites not aligning to the query:

34:40 AKR2A-binding sequence (ABS) required for mitochondrion outer membrane targeting
78 L→F: In cbr1-1; decreased stability and decreased activity.

P27320 Ferredoxin-1; Ferredoxin I from Synechocystis sp. (strain PCC 6803 / Kazusa) (see 3 papers)
48% identity, 18% coverage: 282:344/348 of query aligns to 25:87/97 of P27320

query
sites
P27320

I

I

L

L

D

D

V

A

A

A

L

E

E

E

N

A

K

G

I

L

K

D

L

L

P

P

Y

Y

S

S

C
|
C

R

R

G

A

G

G

V

A

C
|
C

S

S

T

T

C
|
C

V

A

A

G

N

K

C

I

T

T

K

A

G

G

G

S

V

V

R

D

M

Q

D

S

Y

D

N

Q

E

S

V

F

L

L

T

D

D

D

E

Q

L

I

E

E

R

A

G

G

R

Y

V

V

L

L

V

T

C
|
C

T

V

G

A

H

Y

P

P

T

T

E

S

N

D

G

C

T

T

C40 (= C297) binding
C45 (= C302) binding
C48 (= C305) binding
C78 (= C335) binding

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

5aukA Crystal structure of the ga-substituted ferredoxin (see paper)
48% identity, 18% coverage: 282:344/348 of query aligns to 24:86/96 of 5aukA

query
sites
5aukA

I

I

L

L

D

D

V

A

A

A

L

E

E

E

N

A

K

G

I

L

K

D

L

L

P

P

Y
|
Y

S
|
S

C
|
C

R
|
R

G

A

G
|
G

V

A

C
|
C

S

S

T

T

C
|
C

V

A

A

G

N

K

C

I

T

T

K

A

G

G

G

S

V

V

R

D

M

Q

D

S

Y

D

N

Q

E

S

V

F

L

L

T

D

D

D

E

Q

L

I

E

E

R

A

G

G

R

Y

V

V

L

L

V

T

C
|
C

T

V

G

A

H

Y

P

P

T

T

E

S

N

D

G

C

T

T

binding [2Ga-2S] cluster: Y37 (= Y295), S38 (= S296), C39 (= C297), R40 (= R298), G42 (= G300), C44 (= C302), C47 (= C305), C77 (= C335)

2kajA Nmr structure of gallium substituted ferredoxin (see paper)
48% identity, 18% coverage: 282:344/348 of query aligns to 24:86/96 of 2kajA

query
sites
2kajA

I

I

L

L

D

D

V

A

A

A

L

E

E

E

N

A

K

G

I

L

K

D

L

L

P

P

Y

Y

S

S

C
|
C

R

R

G

A

G

G

V

A

C
|
C

S

S

T

T

C
|
C

V

A

A

G

N

K

C

I

T

T

K

A

G

G

G

S

V

V

R

D

M

Q

D

S

Y

D

N

Q

E

S

V

F

L

L

T

D

D

D

E

Q

L

I

E

E

R

A

G

G

R

Y

V

V

L

L

V

T

C
|
C

T

V

G

A

H

Y

P

P

T

T

E

S

N

D

G

C

T

T

binding gallium (iii) ion: C39 (= C297), C44 (= C302), C47 (= C305), C77 (= C335)

1doxA 1h and 15n sequential assignment, secondary structure and tertiary fold of [2fe-2s] ferredoxin from synechocystis sp. Pcc 6803 (see paper)
48% identity, 18% coverage: 282:344/348 of query aligns to 24:86/96 of 1doxA

query
sites
1doxA

I

I

L

L

D

D

V

A

A

A

L

E

E

E

N

A

K

G

I

L

K

D

L

L

P

P

Y

Y

S

S

C
|
C

R

R

G

A

G

G

V

A

C
|
C

S

S

T

T

C
|
C

V

A

A

G

N

K

C

I

T

T

K

A

G

G

G

S

V

V

R

D

M

Q

D

S

Y

D

N

Q

E

S

V

F

L

L

T

D

D

D

E

Q

L

I

E

E

R

A

G

G

R

Y

V

V

L

L

V

T

C
|
C

T

V

G

A

H

Y

P

P

T

T

E

S

N

D

G

C

T

T

binding fe2/s2 (inorganic) cluster: C39 (= C297), C44 (= C302), C47 (= C305), C77 (= C335)

6laaA Crystal structure of full-length cyp116b46 from tepidiphilus thermophilus (see paper)
25% identity, 89% coverage: 27:335/348 of query aligns to 466:739/753 of 6laaA

query
sites
6laaA

P

P

Y

L

P

P

K

R

Y

W

L

S

A

P

G

G

Q

A

F

H

I

I

S

D

L

I

I

-

F

-

Q

E

G

C

K

G

H

E

K

P

E

D

I

R

R

S

R
|
R

S
x
Q

Y
|
Y

S
|
S

F

L

N

C

S

S

S

D

P

P

D

E

V

N

D

R

E

D

P

A

L

W

S

R

I

V

T

A

V

V

K
x
Q

R

R

-

D

-

P

V

A

E
x
S

N
x
R

G

G

E
x
G

I

-

S
|
S

R

R

F

W

L

I

H

H

H

E

K

E

T

V

S

R

V

P

N

G

D

M

I

L

L

L

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R

A

V

Q

R

E

G

P

P

Q

R

G

N

M

S

F

F

S

R

Y

L

L

-

P

-

D

D

E

E

N

H

L

A

E

P

R

R

D

Y

L

L

F

F

L

L

F

-

A

A

A

G

G

G

V
x
I

G

G

I

I

T

T

P

P

L

I

F

M

S

T

I

M

L

-

K

-

T

A

A

A

L

R

V

A

R

K

E

E

K

L

K

G

S

T

L

D

V

Y

T

E

L

L

V

H

Y

Y

S

S

N

V

R

R

S

S

M

R

E

T

D

S

A

L

L

I

F

F

Y

V

D

D

E

E

L

L

S

-

E

-

W

-

Q

R

K

Q

K

I

Y

H

P

G

E

D

R

R

L

L

K

H

I

V

V

Y

W

V

V

S

F

E

S

E

N

G

S

V

K

R

N

N

L

D

M

L

T

A

A

A

R

L

L

I

N

R

K

R

F

A

Y

S

I

A

E

-

K

-

L

-

K

-

E

-

H

-

L

-

H

-

F

-

D

-

R

-

N

-

D

G

A

T

L

Q

F

I

Y

Y

S

A

C

C

G

G

P

P

I

Q

I

R

Y

M

M

L

D

D

L

T

C

-

R

-

I

L

T

E

L

R

L

L

G

I

V

E

G

N

F

R

D

P

I

E

K

V

Q

T

I

L

K

R

R

V

E
|
E

T

H

F
|
F

V

F

L

G

P

E

E

P

D

S

E

H

V

L

D

D

E

P

D

-

G

-

S

-

A

-

E

-

K

-

V

-

V

A

D

K

K

E

N

R

T

P

Y

F

S

Q

V

V

L

L

N

R

F

N

K

S

G

G

E

-

I

-

Y

L

H

T

L

V

D

E

V

V

P

P

W

A

P

D

K

K

R

T

I

L

L

L

D

E

V

V

A

L

L

R

E

A

N

Y

K

N

I

I

K

E

L

V

P

Q

Y
x
S

S

D

C
|
C

R
x
E

G

E

G
|
G

V

L

C
|
C

S
x
G

T

T

C
|
C

V

E

A

V

N

S

C

V

T

V

K

E

G

G

G

E

V

V

R

-

M

-

D

D

Y

H

N

R

E

D

-

S

V

V

L

L

T

T

D

R

D

A

E

E

L

R

E

R

R

E

G

N

R

R

V

R

L

M

V

M

C

C

binding fe2/s2 (inorganic) cluster: S700 (≠ Y295), C702 (= C297), E703 (≠ R298), G705 (= G300), C707 (= C302), G708 (≠ S303), C710 (= C305)
binding flavin mononucleotide: R487 (= R50), Q488 (≠ S51), Y489 (= Y52), S490 (= S53), Q506 (≠ K69), S511 (≠ E72), R512 (≠ N73), G514 (≠ E75), S515 (= S77), I553 (≠ V118), E651 (= E236), F653 (= F238)

Sites not aligning to the query:

active site: 177, 251, 252, 359, 360, 361
binding carbonate ion: 90, 91, 92, 241
binding fe2/s2 (inorganic) cluster: 740
binding protoporphyrin ix containing fe: 54, 91, 92, 99, 103, 249, 252, 253, 298, 351, 352, 353, 357, 359, 361

4fxcA Tertiary structure of [2fe-2s] ferredoxin from spirulina platensis refined at 2.5 angstroms resolution: structural comparisons of plant-type ferredoxins and an electrostatic potential analysis (see paper)
46% identity, 18% coverage: 282:344/348 of query aligns to 26:88/98 of 4fxcA

query
sites
4fxcA

I

I

L

L

D

D

V

A

A

A

L

E

E

E

N

A

K

G

I

L

K

D

L

L

P

P

Y
|
Y

S
|
S

C
|
C

R
|
R

G

A

G
|
G

V

A

C
|
C

S

S

T

T

C
|
C

V

A

A

G

N

T

C

I

T

T

K

S

G

G

G

T

V

I

R

D

M

Q

D

S

Y

D

N

Q

E

S

V

F

L

L

T

D

D

D

E

Q

L

I

E

E

R

A

G

G

R

Y

V

V

L
|
L

V

T

C
|
C

T

V

G

A

H

Y

P

P

T

T

E

S

N

D

G

C

T

T

binding fe2/s2 (inorganic) cluster: Y39 (= Y295), S40 (= S296), C41 (= C297), R42 (= R298), G44 (= G300), C46 (= C302), C49 (= C305), L77 (= L333), C79 (= C335)

P0A3C7 Ferredoxin-1; Ferredoxin I from Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576) (see paper)
39% identity, 24% coverage: 260:342/348 of query aligns to 3:87/99 of P0A3C7

query
sites
P0A3C7

T

T

Y

F

S

K

V

V

V

T

L

L

-

I

-

N

N

E

F

A

K

E

G

G

E

T

I

K

Y

H

H

E

L

I

D

E

V

V

P

P

W

D

P

D

K

E

R

Y

I

I

L

L

D

D

V

A

A

A

L

E

E

E

N

Q

K

G

I

Y

K

D

L

L

P

P

Y

F

S

S

C

C

R
|
R

G

A

G

G

V

A

C

C

S

S

T
|
T

C

C

V

A

A

G

N

K

C

L

T

V

K

S

G

G

G

T

V

V

R

D

M

Q

D

S

Y

D

N

Q

E

S

V
x
F

L

L

T

D

D
|
D

E

Q

L

I

E

E

R

A

G

G

R

Y

V

V

L

L

V

T

C

C

T

V

G

A

H

Y

P

P

T

T

E

S

N

D

R43 (= R298) mutation R->A,E,H: No effect on electron transfer.
T49 (= T304) mutation to A: No effect on electron transfer.
F66 (≠ V321) mutation F->A,I: 1000-fold decrease in electron transfer.
D69 (= D324) mutation to K: Small effect on electron transfer.
D70 (= D325) mutation to K: Small effect on electron transfer.

Sites not aligning to the query:

95 E→K: 20000-fold decrease in electron transfer.

1ewyC Anabaena pcc7119 ferredoxin:ferredoxin-NADP+-reductase complex (see paper)
39% identity, 24% coverage: 260:342/348 of query aligns to 2:86/98 of 1ewyC

query
sites
1ewyC

T

T

Y

F

S

K

V

V

V

T

L

L

-

I

-

N

N

E

F

A

K

E

G

G

E

T

I

K

Y

H

H

E

L

I

D

E

V

V

P

P

W

D

P

D

K

E

R

Y

I

I

L

L

D

D

V

A

A

A

L

E

E

E

N

Q

K

G

I

Y

K

D

L

L

P

P

Y
x
F

S
|
S

C
|
C

R
|
R

G

A

G
|
G

V
x
A

C
|
C

S

S

T

T

C
|
C

V

A

A

G

N

K

C

L

T

V

K

S

G

G

G

T

V

V

R

D

M

Q

D

S

Y

D

N

Q

E
x
S

V

F

L

L

T

D

D

D

E

Q

L

I

E

E

R

A

G

G

R

Y

V

V

L
|
L

V

T

C
|
C

T

V

G

A

H

Y

P

P

T

T

E

S

N

D

binding flavin-adenine dinucleotide: A45 (≠ V301), S64 (≠ E320)
binding fe2/s2 (inorganic) cluster: F39 (≠ Y295), S40 (= S296), C41 (= C297), R42 (= R298), G44 (= G300), A45 (≠ V301), C46 (= C302), C49 (= C305), L77 (= L333), C79 (= C335)

Query Sequence

>CA265_RS25075 FitnessBrowser__Pedo557:CA265_RS25075
MFKLRINKIINQPGDNITFQFEEVDEPYPKYLAGQFISLIFQGKHKEIRRSYSFNSSPDV
DEPLSITVKRVENGEISRFLHHKTSVNDILLAQEPQGMFSYLPDENLERDLFLFAAGVGI
TPLFSILKTALVREKKSLVTLVYSNRSMEDALFYDELSEWQKKYPERLKIVWVFSNSKNL
MTARLNKFYIEKLLKEHLHFDRNDALFYSCGPIIYMDLCRITLLGVGFDIKQIKRETFVL
PEDEVDEDDGSAEKVVDKNTYSVVLNFKGEIYHLDVPWPKRILDVALENKIKLPYSCRGG
VCSTCVANCTKGGVRMDYNEVLTDDELERGRVLVCTGHPTENGTTIAW

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory