SitesBLAST

P10880 Shikimate kinase 2; SK 2; Shikimate kinase II; SKII; EC 2.7.1.71 from Dickeya chrysanthemi (Pectobacterium chrysanthemi) (Erwinia chrysanthemi) (see paper)
34% identity, 89% coverage: 3:151/167 of query aligns to 5:153/173 of P10880

query
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P10880

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C13 (= C11) mutation to S: 66% of wild-type activity. Increase in substrates affinity.
K15 (= K13) mutation to M: Loss of activity. Increased thermostability.
D34 (= D32) mutation to N: Loss of activity.

Sites not aligning to the query:

162 C→S: No effect on activity and substrates affinity.

2shkB The three-dimensional structure of shikimate kinase from erwinia chrysanthemi complexed with adp (see paper)
35% identity, 89% coverage: 3:151/167 of query aligns to 5:143/162 of 2shkB

query
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2shkB

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binding adenosine-5'-diphosphate: G12 (= G10), C13 (= C11), G14 (= G12), K15 (= K13), T16 (≠ S14), T17 (= T15), R110 (= R109)
binding magnesium ion: Q134 (≠ T143), A137 (= A145)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 145, 147

1e6cA K15m mutant of shikimate kinase from erwinia chrysanthemi (see paper)
34% identity, 89% coverage: 3:151/167 of query aligns to 5:153/170 of 1e6cA

query
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1e6cA

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binding phosphate ion: R11 (≠ M9), G12 (= G10), M15 (≠ K13), T16 (≠ S14)

1shkA The three-dimensional structure of shikimate kinase from erwinia chrysanthemi (see paper)
40% identity, 58% coverage: 3:99/167 of query aligns to 5:100/158 of 1shkA

query
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1shkA

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binding magnesium ion: R92 (≠ N91), A93 (= A92), G95 (= G94)

Sites not aligning to the query:

binding magnesium ion: 129, 132

3bafA Crystal structure of shikimate kinase from mycobacterium tuberculosis in complex with amp-pnp
32% identity, 89% coverage: 2:150/167 of query aligns to 3:148/165 of 3bafA

query
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3bafA

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binding phosphoaminophosphonic acid-adenylate ester: P10 (≠ M9), G13 (= G12), K14 (= K13), S15 (= S14), T16 (= T15), R109 (= R109), R116 (= R116)
binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: D33 (= D32), R135 (= R138)

2dfnA Structure of shikimate kinase from mycobacterium tuberculosis complexed with adp and shikimate at 1.9 angstrons of resolution (see paper)
32% identity, 89% coverage: 2:150/167 of query aligns to 3:148/165 of 2dfnA

query
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2dfnA

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binding adenosine-5'-diphosphate: G11 (= G10), S12 (≠ C11), G13 (= G12), K14 (= K13), S15 (= S14), T16 (= T15), R109 (= R109), R116 (= R116)
binding chloride ion: P10 (≠ M9), K14 (= K13), G79 (= G78)
binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: D33 (= D32), I44 (= I43), F56 (= F55), R57 (= R56), G78 (= G77), G79 (= G78), G80 (= G79), R135 (= R138)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 152, 154

1we2A Crystal structure of shikimate kinase from mycobacterium tuberculosis in complex with mgadp and shikimic acid (see paper)
32% identity, 89% coverage: 2:150/167 of query aligns to 3:148/165 of 1we2A

query
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1we2A

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binding adenosine-5'-diphosphate: P10 (≠ M9), G11 (= G10), G13 (= G12), K14 (= K13), S15 (= S14), T16 (= T15), R109 (= R109), R116 (= R116)
binding 3-dehydroshikimate: D33 (= D32), I44 (= I43), F48 (= F47), G78 (= G77), G79 (= G78), G80 (= G79), L118 (= L118), R135 (= R138)

4bqsA Crystal structure of mycobacterium tuberculosis shikimate kinase in complex with adp and a shikimic acid derivative. (see paper)
32% identity, 89% coverage: 2:150/167 of query aligns to 3:148/169 of 4bqsA

query
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P

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L

Y

Y

T

R

R

R

-

V

A

A

K

T

L

M

I

R

V

V

D

D

binding adenosine-5'-diphosphate: P10 (≠ M9), G11 (= G10), G13 (= G12), K14 (= K13), S15 (= S14), T16 (= T15), R109 (= R109)
binding (1R,6R,10S)-6,10-dihydroxy-2-oxabicyclo[4.3.1]deca-4(Z),7-diene-8-carboxylic acid: D33 (= D32), R135 (= R138)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 152, 154

2iyyA Shikimate kinase from mycobacterium tuberculosis in complex with shikimate-3-phosphate and so4 (see paper)
32% identity, 89% coverage: 2:150/167 of query aligns to 3:148/169 of 2iyyA

query
sites
2iyyA

K

K

I

A

F

V

L

L

I

V

G

G

Y

L

M

P

G

G

C

S

G

G

K
|
K

S

S

T

T

K

I

A

G

K

R

Q

R

L

L

A

A

H

K

R

A

L

L

D

G

C

V

P

G

V

L

I

L

D

D

L

T

D
|
D

A

V

E

A

I

I

V
x
E

S

Q

K

R

T

T

G

G

K

R

S
|
S

I
|
I

A

A

E

D

Y

I

F

F

A

A

E

T

Y

D

G

G

E

E

S

Q

G

E

F

F

R
|
R

D

R

Y

I

E

E

S

E

E

D

M

V

L

V

K

R

T

A

F

A

D

L

Y

A

P

D

E

H

T

D

C

G

V

V

V

L

A

S

T

L

G

G

G
|
G

L

-

P

A

C

V

F

T

F

S

D

P

N

G

M

V

E

R

W

A

M

A

N

L

A

A

N

G

G

H

E

T

T

V

V

V

Y

Y

L

L

Q

E

M

I

E

S

P

A

A

A

A

E

L

G

V

V

S

R

R

R

L

T

H

G

N

G

R

N

Q

T

K

V

R

R

P

P

L
|
L

I

L

K

A

D

G

L

P

D

D

D

R

E

A

Q

E

L

-

V

-

F

K

I

Y

K

R

E

A

K

L

L

M

E

A

E

K

R
|
R

D

A

P

P

F

L

Y

Y

T

R

R

R

-

V

A

A

K

T

L

M

I

R

V

V

D

D

binding magnesium ion: E37 (≠ V36), S43 (= S42), I44 (= I43)
binding phosphate ion: K14 (= K13), G79 (= G78)
binding shikimate-3-phosphate: K14 (= K13), D33 (= D32), R57 (= R56), G79 (= G78), G80 (= G79), L118 (= L118), R135 (= R138)

2iywA Shikimate kinase from mycobacterium tuberculosis in complex with mgatp, open lid (conf. B) (see paper)
32% identity, 89% coverage: 2:150/167 of query aligns to 3:148/178 of 2iywA

query
sites
2iywA

K

K

I

A

F

V

L

L

I

V

G

G

Y

L

M

P

G
|
G

C

S

G
|
G

K
|
K

S
|
S

T
|
T

K

I

A

G

K

R

Q

R

L

L

A

A

H

K

R

A

L

L

D

G

C

V

P

G

V

L

I

L

D

D

L

T

D

D

A

V

E

A

I

I

V

E

S

Q

K

R

T

T

G

G

K

R

S

S

I

I

A

A

E

D

Y

I

F

F

A

A

E

T

Y

D

G

G

E

E

S

Q

G

E

F

F

R

R

D

R

Y

I

E

E

S

E

E

D

M

V

L

V

K

R

T

A

F

A

D

L

Y

A

P

D

E

H

T

D

C

G

V

V

V

L

A

S

T

L

G

G

L

A

P

-

C

V

F

T

F

S

D

P

N

G

M

V

E

R

W

A

M

A

N

L

A

A

N

G

G

H

E

T

T

V

V

V

Y

Y

L

L

Q

E

M

I

E

S

P

A

A

A

A

E

L

G

V

V

S

R

R
|
R

L

T

H

G

N

G

R

N

Q

T

K

V

R

R

P

P

L

L

I

L

K

A

D

G

L

P

D

D

D

R

E

A

Q

E

L

-

V

-

F

K

I

Y

K

R

E

A

K

L

L

M

E

A

E

K

R

R

D

A

P

P

F

L

Y

Y

T

R

R

R

-

V

A

A

K

T

L

M

I

R

V

V

D

D

binding adenosine-5'-triphosphate: G11 (= G10), G13 (= G12), K14 (= K13), S15 (= S14), T16 (= T15), R109 (= R109)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 152, 154

1zyuA Crystal structure of mycobacterium tuberculosis shikimate kinase in complex with shikimate and amppcp at 2.85 angstrom resolution (see paper)
32% identity, 89% coverage: 2:150/167 of query aligns to 3:148/168 of 1zyuA

query
sites
1zyuA

K

K

I

A

F

V

L

L

I

V

G

G

Y

L

M
x
P

G
|
G

C

S

G
|
G

K
|
K

S
|
S

T
|
T

K

I

A

G

K

R

Q

R

L

L

A

A

H

K

R

A

L

L

D

G

C

V

P

G

V

L

I

L

D

D

L

T

D
|
D

A

V

E

A

I

I

V

E

S

Q

K

R

T

T

G

G

K

R

S

S

I

I

A

A

E

D

Y

I

F

F

A

A

E

T

Y

D

G

G

E

E

S

Q

G

E

F

F

R
|
R

D

R

Y

I

E

E

S

E

E

D

M

V

L

V

K

R

T

A

F

A

D

L

Y

A

P

D

E

H

T

D

C

G

V

V

V

L

A

S

T

L

G

G

G
|
G

L

-

P

A

C

V

F

T

F

S

D

P

N

G

M

V

E

R

W

A

M

A

N

L

A

A

N

G

G

H

E

T

T

V

V

V

Y

Y

L

L

Q

E

M

I

E

S

P

A

A

A

A

E

L

G

V

V

S

R

R
|
R

L

T

H

G

N

G

R

N

Q

T

K

V

R

R

P
|
P

L
|
L

I

L

K

A

D

G

L

P

D

D

D

R

E

A

Q

E

L

-

V

-

F

K

I

Y

K

R

E

A

K

L

L

M

E

A

E

K

R
|
R

D

A

P

P

F

L

Y

Y

T

R

R

R

-

V

A

A

K

T

L

M

I

R

V

V

D

D

binding phosphomethylphosphonic acid adenylate ester: P10 (≠ M9), G11 (= G10), G13 (= G12), K14 (= K13), S15 (= S14), T16 (= T15), R109 (= R109)
binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: D33 (= D32), R57 (= R56), G80 (= G79), P117 (= P117), L118 (= L118), R135 (= R138)

Sites not aligning to the query:

binding phosphomethylphosphonic acid adenylate ester: 149, 152, 154

2iyvA Shikimate kinase from mycobacterium tuberculosis in complex with adp, open lid (conf. B) (see paper)
32% identity, 89% coverage: 2:150/167 of query aligns to 3:148/179 of 2iyvA

query
sites
2iyvA

K

K

I

A

F

V

L

L

I

V

G

G

Y

L

M

P

G
|
G

C

S

G
|
G

K
|
K

S
|
S

T
|
T

K

I

A

G

K

R

Q

R

L

L

A

A

H

K

R

A

L

L

D

G

C

V

P

G

V

L

I

L

D

D

L

T

D

D

A

V

E

A

I

I

V

E

S

Q

K

R

T

T

G

G

K

R

S

S

I

I

A

A

E

D

Y

I

F

F

A

A

E

T

Y

D

G

G

E

E

S

Q

G

E

F

F

R

R

D

R

Y

I

E

E

S

E

E

D

M

V

L

V

K

R

T

A

F

A

D

L

Y

A

P

D

E

H

T

D

C

G

V

V

V

L

A

S

T

L

G

G

L

A

P

-

C

V

F

T

F

S

D

P

N

G

M

V

E

R

W

A

M

A

N

L

A

A

N

G

G

H

E

T

T

V

V

V

Y

Y

L

L

Q

E

M

I

E

S

P

A

A

A

A

E

L

G

V

V

S

R

R
|
R

L

T

H

G

N

G

R

N

Q

T

K

V

R

R

P

P

L

L

I

L

K

A

D

G

L

P

D

D

D

R

E

A

Q

E

L

-

V

-

F

K

I

Y

K

R

E

A

K

L

L

M

E

A

E

K

R

R

D

A

P

P

F

L

Y

Y

T

R

R

R

-

V

A

A

K

T

L

M

I

R

V

V

D

D

binding adenosine-5'-diphosphate: G11 (= G10), G13 (= G12), K14 (= K13), S15 (= S14), T16 (= T15), R109 (= R109)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 152, 154

1u8aA Crystal structure of mycobacterium tuberculosis shikimate kinase in complex with shikimate and adp at 2.15 angstrom resolution (see paper)
32% identity, 89% coverage: 2:150/167 of query aligns to 3:146/163 of 1u8aA

query
sites
1u8aA

K

K

I

A

F

V

L

L

I

V

G

G

Y

L

M

P

G
|
G

C

S

G
|
G

K
|
K

S
|
S

T
|
T

K

I

A

G

K

R

Q

R

L

L

A

A

H

K

R

A

L

L

D

G

C

V

P

G

V

L

I

L

D

D

L

T

D
|
D

A

V

E

A

I

I

V

E

S

Q

K

R

T

T

G

G

K

R

S

S

I

I

A

A

E

D

Y

I

F
|
F

A

A

E

T

Y

D

G

G

E

E

S

Q

G

E

F

F

R
|
R

D

R

Y

I

E

E

S

E

E

D

M

V

L

V

K

R

T

A

F

A

D

L

Y

A

P

D

E

H

T

D

C

G

V

V

V

L

A

S

T

L

G

G

G
|
G

L

-

P

A

C

V

F

T

F

S

D

P

N

G

M

V

E

R

W

A

M

A

N

L

A

A

N

G

G

H

E

T

T

V

V

V

Y

Y

L

L

Q

E

M

I

E

S

P

A

A

A

A

E

L

G

V

V

S

R

R
|
R

L

T

H

G

N

G

R

-

Q

-

K

V

R

R

P

P

L
|
L

I

L

K

A

D

G

L

P

D

D

D

R

E

A

Q

E

L

-

V

-

F

K

I

Y

K

R

E

A

K

L

L

M

E

A

E

K

R
|
R

D

A

P

P

F

L

Y

Y

T

R

R

R

-

V

A

A

K

T

L

M

I

R

V

V

D

D

binding adenosine-5'-diphosphate: G11 (= G10), G13 (= G12), K14 (= K13), S15 (= S14), T16 (= T15), R109 (= R109)
binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: D33 (= D32), F48 (= F47), R57 (= R56), G79 (= G78), G80 (= G79), L116 (= L118), R133 (= R138)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 150, 152

6hqvA Pentafunctional arom complex from chaetomium thermophilum (see paper)
35% identity, 47% coverage: 2:80/167 of query aligns to 844:925/1555 of 6hqvA

query
sites
6hqvA

K

S

I

I

F

F

L

I

I

V

G

G

Y

M

M

R

G

G

C

A

G

G

K

K

S

S

T

T

K

A

A

G

K

R

Q

W

L

M

A

S

H

E

R

L

L

L

D

K

C

R

P

P

V

L

I

V

D

D

L

L

D
|
D

A

A

E

E

I

L

V

E

S

R

K

R

T

E

G

G

K

M

S

T

I

I

A

P

E

E

Y

I

F

I

-

R

A

G

E

E

Y

R

G

G

E

W

S

E

G

G

F

F

R

R

D

Q

Y

A

E

E

S

L

E

E

M

L

L

L

K

Q

T

D

F

V

-

I

-

K

D

N

Y

Q

P

S

E

K

T

G

C

Y

V

I

V

F

A

S

T

C

G

G

G
|
G

L

I

binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: D874 (= D32), G923 (= G78), G924 (= G79)

Sites not aligning to the query:

active site: 123, 145, 187, 243, 253, 257, 261, 264, 268, 280
binding (4S,5R)-4,5-dihydroxy-3-oxocyclohex-1-ene-1-carboxylic acid: 1060, 1062, 1181, 1224, 1232, 1242, 1243
binding glutamic acid: 139, 145, 187, 243, 257, 264, 280
binding nicotinamide-adenine-dinucleotide: 42, 44, 45, 76, 79, 107, 108, 109, 112, 132, 133, 135, 139, 140, 145, 154, 175, 176, 177, 180, 280
binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: 413, 562, 563, 979, 1277, 1279, 1323, 1327, 1348, 1368, 1526
binding zinc ion: 187, 264, 280

P07547 Pentafunctional AROM polypeptide; EC 4.2.3.4; EC 2.5.1.19; EC 2.7.1.71; EC 4.2.1.10; EC 1.1.1.25 from Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) (see 2 papers)
36% identity, 47% coverage: 2:80/167 of query aligns to 865:945/1583 of P07547

query
sites
P07547

K

S

I

I

F

Y

L

I

I

I

G

G

Y

M

M

R

G

G

C

A

G

G

K

K

S

S

T

T

K

A

A

G

K

N

Q

W

L

V

A

S

H

K

R

A

L

L

D

N

C

R

P

P

V

F

I

V

D

D

L

L

D

D

A

T

E

E

I

L

V

E

S

T

K

V

T

E

G

G

K

M

S

T

I

I

A

P

E

D

Y

I

F

I

A

K

E

T

Y

R

G

G

E

W

S

Q

G

G

F

F

R

R

D

N

Y

A

E

E

S

L

E

E

M

I

L

L

K

K

T

R

F

T

-

L

-

K

D

E

Y

R

P

S

E

R

T

G

C

Y

V

V

V

F

A

A

T

C

G

G

L

V

Sites not aligning to the query:

44:46 binding
81:84 binding
114:116 binding
119 binding
139:140 binding
161 binding
179:182 binding
190 binding
194 binding
271 binding
287 binding

Query Sequence

>CA265_RS25310 FitnessBrowser__Pedo557:CA265_RS25310
MKIFLIGYMGCGKSTKAKQLAHRLDCPVIDLDAEIVSKTGKSIAEYFAEYGESGFRDYES
EMLKTFDYPETCVVATGGGLPCFFDNMEWMNANGETVYLQMEPAALVSRLHNRQKRPLIK
DLDDEQLLVFIKEKLEERDPFYTRAKLIVDAFDLDGEKLEEAIKKNR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory