SitesBLAST
Comparing CCNA_00083 CCNA_00083 phosphoglucomutase/phosphomannomutase to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
2fuvA Phosphoglucomutase from salmonella typhimurium.
60% identity, 100% coverage: 2:545/546 of query aligns to 2:543/545 of 2fuvA
Q9VUY9 Phosphoglucomutase; PGM; Glucose phosphomutase; EC 5.4.2.2 from Drosophila melanogaster (Fruit fly) (see 4 papers)
27% identity, 71% coverage: 65:450/546 of query aligns to 41:429/560 of Q9VUY9
- S116 (= S145) modified: Phosphoserine
- E351 (≠ R366) natural variant: E -> K
Sites not aligning to the query:
- 6 natural variant: E -> G
- 17 natural variant: K -> Q
- 28 natural variant: K -> N
- 36 natural variant: T -> M
6snoA Crystal structures of human pgm1 isoform 2 (see paper)
28% identity, 75% coverage: 42:450/546 of query aligns to 31:442/573 of 6snoA
- active site: R36 (= R47), S130 (= S145), H131 (= H146), K143 (= K155), D301 (= D304), D303 (= D306), D305 (= D308), R306 (= R309), G393 (= G395)
- binding 1-O-phosphono-alpha-D-glucopyranose: S33 (= S44), S130 (= S145), R306 (= R309), T370 (≠ V372), E389 (= E391), S391 (= S393)
- binding zinc ion: S130 (= S145), D301 (= D304), D303 (= D306), D305 (= D308)
Sites not aligning to the query:
6snqA Crystal structures of human pgm1 isoform 2 (see paper)
28% identity, 75% coverage: 42:450/546 of query aligns to 31:442/566 of 6snqA
- active site: R36 (= R47), S130 (= S145), H131 (= H146), K143 (= K155), D301 (= D304), D303 (= D306), D305 (= D308), R306 (= R309), G393 (= G395)
- binding 6-O-phosphono-alpha-D-glucopyranose: S130 (= S145), R306 (= R309), T370 (≠ V372), G371 (= G373), E389 (= E391), S391 (= S393)
- binding zinc ion: S130 (= S145), D301 (= D304), D303 (= D306), D305 (= D308)
Sites not aligning to the query:
P36871 Phosphoglucomutase-1; PGM 1; Glucose phosphomutase 1; EC 5.4.2.2 from Homo sapiens (Human) (see 11 papers)
27% identity, 78% coverage: 24:450/546 of query aligns to 11:429/562 of P36871
- T19 (≠ V32) to A: in CDG1T; reduces strongly phosphoglucomutase activity; dbSNP:rs1320810473
- N38 (≠ A58) to Y: in CDG1T; reduces strongly solubility; increases aggregation; dbSNP:rs587777402
- Q41 (≠ L61) to R: in CDG1T; reduces solubility; increases aggregation; dbSNP:rs1300651770
- D62 (= D85) to H: in CDG1T; reduces solubility; reduces strongly phosphoglucomutase activity; dbSNP:rs587777403
- K68 (≠ E91) to M: in allele PGM1*7+, allele PGM1*7-, allele PGM1*3+ and allele PGM1*3-; phosphoglucomutase activity is similar to wild-type; dbSNP:rs200390982
- T115 (= T143) to A: in CDG1T; reduces mildly phosphoglucomutase activity; dbSNP:rs121918371
- S117 (= S145) active site, Phosphoserine intermediate; binding via phosphate groupe; modified: Phosphoserine
- G121 (vs. gap) to R: in CDG1T; there is 7% enzyme residual phosphoglucomutase activity; dbSNP:rs398122912
- R221 (≠ G229) to C: in allele PGM1*2+, allele PGM1*2-, allele PGM1*3+ and allele PGM1*3-; phosphoglucomutase activity is similar to wild-type; dbSNP:rs1126728
- D263 (= D277) to G: in CDG1T; reduces strongly phosphoglucomutase activity; dbSNP:rs1465877146; to Y: in CDG1T; reduces strongly phosphoglucomutase activity; dbSNP:rs587777404
- D288 (= D304) binding
- D290 (= D306) binding
- G291 (≠ A307) to R: in CDG1T; reduces strongly phosphoglucomutase activity; dbSNP:rs772768778
- D292 (= D308) binding
- G330 (= G347) to R: in CDG1T; decreases mildly solubility; dbSNP:rs777164338
- E377 (= E392) to K: in CDG1T; decreases strongly solubility
- E388 (≠ D409) to K: in CDG1T; decreases strongly solubility; dbSNP:rs1301021797
- Y420 (= Y441) to H: in allele PGM1*1-, allele PGM1*2-, allele PGM1*3- and allele PGM1*7-; phosphoglucomutase activity is similar to wild-type; dbSNP:rs11208257
Sites not aligning to the query:
- 467 modified: Phosphothreonine; by PAK1
- 516 L → P: in CDG1T; decreases strongly solubility; dbSNP:rs587777401
6y8yA Structure of baltic herring (clupea harengus) phosphoglucomutase 5 (pgm5) with bound glucose-1-phosphate (see paper)
27% identity, 70% coverage: 42:425/546 of query aligns to 27:418/572 of 6y8yA
Sites not aligning to the query:
7pjcB The structure of candida albicans phosphoglucomutase with isothiazolone modification on cys359
26% identity, 82% coverage: 28:477/546 of query aligns to 2:456/553 of 7pjcB
- binding ~{N}-(3-chloranyl-2-fluoranyl-phenyl)-3-sulfanyl-propanamide: P134 (= P163), W351 (≠ F374), F354 (= F377), C355 (≠ V378), F358 (≠ L381), C366 (≠ G389), G374 (= G395), S375 (≠ A396), R379 (= R400), E380 (≠ H401), K381 (≠ D402)
5jn5A Crystal structure of the d263y missense variant of human pgm1 (see paper)
27% identity, 78% coverage: 24:450/546 of query aligns to 12:430/559 of 5jn5A
- active site: R24 (≠ A36), S118 (= S145), H119 (= H146), K131 (= K155), D289 (= D304), D291 (= D306), D293 (= D308), R294 (= R309), G381 (= G395), K390 (= K410)
- binding calcium ion: S118 (= S145), D289 (= D304), D291 (= D306), D293 (= D308)
7s0wB Crystal structure of the t337m variant of human pgm-1 (see paper)
28% identity, 74% coverage: 24:425/546 of query aligns to 12:410/499 of 7s0wB
P00949 Phosphoglucomutase-1; PGM 1; Glucose phosphomutase 1; EC 5.4.2.2 from Oryctolagus cuniculus (Rabbit) (see 2 papers)
27% identity, 78% coverage: 24:450/546 of query aligns to 11:429/562 of P00949
- R23 (≠ A36) binding
- S117 (= S145) active site, Phosphoserine intermediate; binding ; binding via phosphate group; modified: Phosphoserine
- D288 (= D304) binding
- D290 (= D306) binding
- D292 (= D308) binding ; binding
- R293 (= R309) binding
- T357 (≠ V372) binding
- E376 (= E391) binding
- S378 (= S393) binding
- K389 (= K410) binding
3pmgA Structure of rabbit muscle phosphoglucomutase at 2.4 angstroms resolution. Use of freezing point depressant and reduced temperature to enhance diffractivity (see paper)
27% identity, 78% coverage: 24:450/546 of query aligns to 10:428/561 of 3pmgA