SitesBLAST
Comparing CCNA_00763 FitnessBrowser__Caulo:CCNA_00763 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 11 hits to proteins with known functional sites (download)
1efpA Electron transfer flavoprotein (etf) from paracoccus denitrificans (see paper)
61% identity, 98% coverage: 2:308/313 of query aligns to 1:307/307 of 1efpA
- binding flavin-adenine dinucleotide: G199 (= G199), R200 (= R200), G201 (≠ A201), S225 (= S226), R226 (= R227), A227 (= A228), Q239 (= Q240), V240 (= V241), G241 (= G242), T243 (= T244), G256 (= G257), I257 (= I258), S258 (= S259), A260 (= A261), Q262 (= Q263), H263 (= H264), N277 (= N278), K278 (= K279), D295 (= D296), L296 (≠ Y297)
2a1uA Crystal structure of the human etf e165betaa mutant (see paper)
54% identity, 98% coverage: 2:308/313 of query aligns to 3:312/315 of 2a1uA
- binding flavin-adenine dinucleotide: G204 (= G199), R205 (= R200), S230 (= S226), R231 (= R227), A232 (= A228), Q244 (= Q240), V245 (= V241), G246 (= G242), T248 (= T244), G261 (= G257), I262 (= I258), S263 (= S259), A265 (= A261), Q267 (= Q263), H268 (= H264), N282 (= N278), K283 (= K279), D300 (= D296), L301 (≠ Y297)
P13804 Electron transfer flavoprotein subunit alpha, mitochondrial; Alpha-ETF from Homo sapiens (Human) (see 6 papers)
54% identity, 98% coverage: 2:308/313 of query aligns to 21:330/333 of P13804
- G116 (= G95) to R: in GA2A; impaired protein stability and loss of electron transfer activity; dbSNP:rs119458971
- T171 (= T150) to I: decreased protein stability; dbSNP:rs1801591
- R223 (= R200) binding
- S248 (= S226) binding
- R249 (= R227) mutation to A: Loss of electron transfer activity.
- VGQT 263:266 (= VGQT 241:244) binding
- T266 (= T244) to M: in GA2A; decreased electron transfer activity; dbSNP:rs119458970
- SGAIQH 281:286 (= SGAIQH 259:264) binding
- N300 (= N278) binding
- DL 318:319 (≠ DY 296:297) binding
Sites not aligning to the query:
- 20:204 Domain I
- 205:333 Domain II
6fahE Molecular basis of the flavin-based electron-bifurcating caffeyl-coa reductase reaction (see paper)
36% identity, 83% coverage: 53:313/313 of query aligns to 121:393/393 of 6fahE
- binding flavin-adenine dinucleotide: L180 (≠ P110), R200 (= R125), M281 (≠ R200), G282 (≠ A201), R307 (= R227), A308 (= A228), Q320 (= Q240), V321 (= V241), G322 (= G242), Q323 (= Q243), T324 (= T244), G337 (= G257), I338 (= I258), S339 (= S259), Q343 (= Q263), H344 (= H264), N358 (= N278), K359 (= K279), L377 (≠ Y297)
Sites not aligning to the query:
- binding iron/sulfur cluster: 7, 10, 13, 17, 18, 35, 36, 37, 38, 41, 45, 49
5ow0A Crystal structure of an electron transfer flavoprotein from geobacter metallireducens (see paper)
38% identity, 79% coverage: 62:308/313 of query aligns to 49:291/292 of 5ow0A
- binding flavin-adenine dinucleotide: G183 (= G199), R184 (= R200), G185 (≠ A201), S209 (= S226), R210 (= R227), Q223 (= Q240), I224 (≠ V241), G225 (= G242), T227 (= T244), G240 (= G257), V241 (≠ I258), S242 (= S259), A244 (= A261), Q246 (= Q263), H247 (= H264), N261 (= N278), K262 (= K279), D279 (= D296), Y280 (= Y297)
5ol2A The electron transferring flavoprotein/butyryl-coa dehydrogenase complex from clostridium difficile (see paper)
35% identity, 99% coverage: 3:311/313 of query aligns to 4:327/331 of 5ol2A
- binding calcium ion: E75 (= E71), D188 (vs. gap)
- binding flavin-adenine dinucleotide: T117 (≠ I111), R136 (= R125), I147 (≠ V136), G216 (= G199), R217 (= R200), G218 (≠ A201), S242 (= S226), R243 (= R227), A244 (= A228), Q256 (= Q240), V257 (= V241), G258 (= G242), T260 (= T244), G273 (= G257), I274 (= I258), S275 (= S259), A277 (= A261), Q279 (= Q263), H280 (= H264), N294 (= N278), K295 (= K279), D312 (= D296), V313 (≠ Y297)
4kpuA Electron transferring flavoprotein of acidaminococcus fermentans: towards a mechanism of flavin-based electron bifurcation (see paper)
34% identity, 79% coverage: 57:304/313 of query aligns to 70:328/338 of 4kpuA
- binding flavin-adenine dinucleotide: L125 (≠ P110), R144 (= R125), I155 (≠ V136), G224 (= G199), R225 (= R200), G226 (≠ A201), S250 (= S226), R251 (= R227), A252 (= A228), Q264 (= Q240), V265 (= V241), G266 (= G242), Q267 (= Q243), S268 (≠ T244), G281 (= G257), I282 (= I258), S283 (= S259), S285 (≠ A261), Q287 (= Q263), H288 (= H264), N302 (= N278), K303 (= K279), D320 (= D296), A321 (≠ Y297)
7koeB Electron bifurcating flavoprotein fix/etfabcx (see paper)
37% identity, 86% coverage: 35:304/313 of query aligns to 44:323/336 of 7koeB
- binding flavin-adenine dinucleotide: T121 (≠ I111), R140 (= R125), T142 (≠ I127), G219 (= G199), K220 (≠ R200), G221 (≠ A201), S245 (= S226), R246 (= R227), A247 (= A228), Q259 (= Q240), V260 (= V241), G261 (= G242), Q262 (= Q243), T263 (= T244), G276 (= G257), S278 (= S259), Q282 (= Q263), H283 (= H264), N297 (= N278), I298 (≠ K279), L316 (≠ Y297)
7qh2A Cryo-em structure of ldh-etfab complex from acetobacterium woodii (see paper)
32% identity, 90% coverage: 28:308/313 of query aligns to 42:331/337 of 7qh2A
- binding flavin-adenine dinucleotide: L125 (vs. gap), T126 (vs. gap), R144 (= R125), I155 (≠ V136), R224 (= R200), G225 (≠ A201), T249 (≠ S226), R250 (= R227), Q263 (= Q240), I264 (≠ V241), G265 (= G242), L266 (≠ Q243), S267 (≠ T244), G280 (= G257), I281 (= I258), S282 (= S259), Q286 (= Q263), N301 (= N278), S302 (≠ K279), D303 (= D280), D319 (= D296), L320 (≠ Y297)
P53571 Electron transfer flavoprotein subunit alpha; Alpha-ETF; Electron transfer flavoprotein large subunit; ETFLS from Methylophilus methylotrophus (Bacterium W3A1) (see 2 papers)
32% identity, 98% coverage: 3:310/313 of query aligns to 4:321/321 of P53571
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
3clrD Crystal structure of the r236a etf mutant from m. Methylotrophus (see paper)
32% identity, 98% coverage: 3:309/313 of query aligns to 3:319/319 of 3clrD
- binding flavin-adenine dinucleotide: G209 (= G199), R210 (= R200), G211 (≠ A201), S235 (= S226), A236 (≠ R227), P237 (≠ A228), Q249 (= Q240), V250 (= V241), G251 (= G242), Q252 (= Q243), S253 (≠ T244), G267 (= G257), I268 (= I258), S269 (= S259), S271 (≠ A261), Q273 (= Q263), H274 (= H264), N288 (= N278), T289 (≠ K279), D306 (= D296), I307 (≠ Y297)
Query Sequence
>CCNA_00763 FitnessBrowser__Caulo:CCNA_00763
MAVLVVADNDNALLRDATHKTVTAALKISGDVDVLVLGKGAKAVADQAAKIAGVRKVLLA
ESDALGHGVAEAQADAVLALAGNYDAILVPATSGGKNFAPRVAAKLDVAPISEIVDVVAA
DTFTRPIYAGNALETVQSSDSKKVITVRPTAFAAAAEGGSASVESVSGADAAKTRFVSEE
MVKSDRPELAAAKIVVSGGRAMGSAEEFQRVIEPLADKLGAAVGASRAAVDAGYAPNDYQ
VGQTGKVVAPQLYVAIGISGAIQHLAGMKDSKVIVAINKDADAPIFQVADFGLVADYKSA
VPELMDALAAAGK
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory