SitesBLAST
Comparing CCNA_00764 FitnessBrowser__Caulo:CCNA_00764 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 14 hits to proteins with known functional sites (download)
1efpB Electron transfer flavoprotein (etf) from paracoccus denitrificans (see paper)
70% identity, 98% coverage: 1:242/248 of query aligns to 1:246/246 of 1efpB
- binding adenosine monophosphate: P6 (= P6), V7 (= V7), D39 (≠ C39), I63 (= I63), V101 (= V97), A119 (≠ M115), G120 (= G116), Q122 (= Q118), A123 (= A119), N126 (≠ G122), M128 (≠ N124), N129 (= N125), T131 (≠ V127)
- binding flavin-adenine dinucleotide: Y13 (= Y13), L182 (= L178)
P38117 Electron transfer flavoprotein subunit beta; Beta-ETF from Homo sapiens (Human) (see 10 papers)
60% identity, 99% coverage: 1:246/248 of query aligns to 4:253/255 of P38117
- A9 (≠ P6) binding
- NPFC 39:42 (= NPFC 36:39) binding
- C66 (≠ I63) binding
- 123:134 (vs. 116:127, 67% identical) binding
- D128 (= D121) to N: in GA2B; decreased protein stability; dbSNP:rs104894678
- T154 (≠ E147) to M: in dbSNP:rs1130426
- R164 (= R157) to Q: in GA2B; reduced electron transfer activity; dbSNP:rs104894677
- E165 (= E158) mutation E->A,Q: Drastically increases interprotein electron transfer rates.
- 183:205 (vs. 176:198, 91% identical) Recognition loop
- L195 (= L188) mutation to A: Severely impaired in complex formation with ACADM.
- K200 (= K193) modified: N6,N6,N6-trimethyllysine; by ETFBKMT; alternate; mutation to R: Does not abolish electron transfer activity. Decreases sensitivity to inhibition by lysine methyltransferase ETFBKMT.
- KAK 200:202 (= KAK 193:195) mutation to RAR: Does not abolish methylation by ETFBKMT.
- KAKK 200:203 (= KAKK 193:196) mutation to RAKR: Does not abolish electron transfer activity. Abolishes sensitivity to inhibition by lysine methyltransferase ETFBKMT.
- K203 (= K196) modified: N6,N6,N6-trimethyllysine; by ETFBKMT; mutation to R: Does not abolish electron transfer activity. Decreases sensitivity to inhibition by lysine methyltransferase ETFBKMT.
Q2TBV3 Electron transfer flavoprotein subunit beta; Beta-ETF from Bos taurus (Bovine) (see paper)
58% identity, 99% coverage: 1:246/248 of query aligns to 4:253/255 of Q2TBV3
- K200 (= K193) modified: N6,N6,N6-trimethyllysine; by ETFBKMT; alternate
- K203 (= K196) modified: N6,N6,N6-trimethyllysine; by ETFBKMT
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 2 modified: N-acetylalanine
2a1uB Crystal structure of the human etf e165betaa mutant (see paper)
59% identity, 99% coverage: 1:246/248 of query aligns to 1:250/252 of 2a1uB
- binding adenosine monophosphate: A6 (≠ P6), V7 (= V7), K8 (= K8), C39 (= C39), C63 (≠ I63), V101 (= V97), L119 (≠ M115), G120 (= G116), Q122 (= Q118), A123 (= A119), D126 (≠ G122), C128 (≠ N124), N129 (= N125), Q130 (≠ A126), T131 (≠ V127)
- binding flavin-adenine dinucleotide: P37 (= P37), L182 (= L178)
Q9DCW4 Electron transfer flavoprotein subunit beta; Beta-ETF from Mus musculus (Mouse) (see paper)
58% identity, 99% coverage: 1:246/248 of query aligns to 4:253/255 of Q9DCW4
- K200 (= K193) modified: N6,N6,N6-trimethyllysine; by ETFBKMT; alternate
- K203 (= K196) modified: N6,N6,N6-trimethyllysine; by ETFBKMT
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 2 modified: N-acetylalanine
6fahB Molecular basis of the flavin-based electron-bifurcating caffeyl-coa reductase reaction (see paper)
33% identity, 100% coverage: 1:248/248 of query aligns to 1:262/262 of 6fahB
- binding flavin-adenine dinucleotide: C6 (≠ P6), A7 (≠ V7), K8 (= K8), D38 (≠ C39), T61 (≠ S62), M62 (≠ I63), A93 (≠ P92), D94 (≠ E93), T95 (≠ P94), T98 (≠ V97), L102 (= L101), G118 (= G116), Q120 (= Q118), G124 (= G122), T126 (≠ N124), A127 (≠ N125), V129 (= V127), T223 (≠ L215)
P53570 Electron transfer flavoprotein subunit beta; Beta-ETF; Electron transfer flavoprotein small subunit; ETFSS from Methylophilus methylotrophus (Bacterium W3A1) (see paper)
34% identity, 84% coverage: 1:209/248 of query aligns to 1:215/264 of P53570
1o96A Structure of electron transferring flavoprotein for methylophilus methylotrophus. (see paper)
34% identity, 84% coverage: 1:209/248 of query aligns to 1:214/261 of 1o96A
- binding adenosine monophosphate: A6 (≠ P6), V7 (= V7), K8 (= K8), D38 (≠ C39), V63 (≠ I63), V99 (= V97), A117 (≠ M115), G118 (= G116), Q120 (= Q118), S121 (≠ A119), Y126 (≠ N124), A127 (≠ N125), T129 (≠ V127)
- binding flavin-adenine dinucleotide: Q120 (= Q118), Q182 (≠ D177)
7koeA Electron bifurcating flavoprotein fix/etfabcx (see paper)
35% identity, 84% coverage: 3:210/248 of query aligns to 4:219/282 of 7koeA
- binding flavin-adenine dinucleotide: C7 (≠ P6), I8 (≠ V7), K9 (= K8), D39 (≠ C39), M62 (≠ I63), A93 (≠ P92), D94 (≠ E93), T95 (≠ P94), T98 (≠ V97), L102 (= L101), T121 (≠ M115), G122 (= G116), A125 (= A119), G128 (= G122), T130 (≠ N124), G131 (≠ N125), V133 (= V127)
5ol2B The electron transferring flavoprotein/butyryl-coa dehydrogenase complex from clostridium difficile (see paper)
33% identity, 85% coverage: 1:210/248 of query aligns to 1:211/260 of 5ol2B
- binding flavin-adenine dinucleotide: C6 (≠ P6), I7 (≠ V7), K8 (= K8), D38 (≠ C39), M61 (≠ I63), A92 (≠ P92), D93 (≠ E93), T94 (≠ P94), T97 (≠ V97), A115 (≠ M115), G116 (= G116), A119 (= A119), G122 (= G122), T124 (≠ N124), A125 (≠ N125), V127 (= V127)
4l2iB Electron transferring flavoprotein of acidaminococcus fermentans: towards a mechanism of flavin-based electron bifurcation (see paper)
33% identity, 83% coverage: 1:205/248 of query aligns to 1:205/263 of 4l2iB
- binding flavin-adenine dinucleotide: C6 (≠ P6), V7 (= V7), K8 (= K8), Y37 (≠ F38), D38 (≠ C39), Y40 (≠ I41), T60 (≠ S62), M61 (≠ I63), A92 (≠ P92), D93 (≠ E93), T94 (≠ P94), T97 (≠ V97), M101 (≠ L101), C116 (≠ M115), G117 (= G116), Q119 (= Q118), A120 (= A119), G123 (= G122), T125 (≠ N124), A126 (≠ N125), V128 (= V127)
- binding nicotinamide-adenine-dinucleotide: M61 (≠ I63), G62 (= G64), P63 (= P65), S85 (≠ T87), D86 (≠ T88), G91 (≠ D91)
4kpuB Electron transferring flavoprotein of acidaminococcus fermentans: towards a mechanism of flavin-based electron bifurcation (see paper)
33% identity, 83% coverage: 1:205/248 of query aligns to 1:205/263 of 4kpuB
- binding flavin-adenine dinucleotide: C6 (≠ P6), V7 (= V7), K8 (= K8), Y37 (≠ F38), D38 (≠ C39), Y40 (≠ I41), M61 (≠ I63), A92 (≠ P92), D93 (≠ E93), T94 (≠ P94), T97 (≠ V97), C116 (≠ M115), G117 (= G116), Q119 (= Q118), A120 (= A119), G123 (= G122), T125 (≠ N124), A126 (≠ N125), V128 (= V127)
7qh2B Cryo-em structure of ldh-etfab complex from acetobacterium woodii (see paper)
28% identity, 85% coverage: 2:213/248 of query aligns to 3:225/265 of 7qh2B
- binding flavin-adenine dinucleotide: C7 (≠ P6), K9 (= K8), D39 (≠ C39), M62 (≠ I63), D94 (≠ E93), V95 (≠ P94), L102 (= L101), G118 (= G116), Q120 (= Q118), T121 (≠ A119), T126 (≠ N124), A127 (≠ N125), V129 (= V127)
5ow0B Crystal structure of an electron transfer flavoprotein from geobacter metallireducens (see paper)
30% identity, 65% coverage: 38:199/248 of query aligns to 38:199/251 of 5ow0B
- binding adenosine monophosphate: D39 (≠ C39), Y62 (≠ I63), I95 (≠ A96), A114 (≠ M115), G115 (= G116), Q117 (= Q118), S118 (≠ A119), R123 (≠ N124), G124 (≠ N125), V125 (≠ A126), V126 (= V127), P127 (≠ G128)
- binding flavin-adenine dinucleotide: Y38 (≠ F38)
Sites not aligning to the query:
Query Sequence
>CCNA_00764 FitnessBrowser__Caulo:CCNA_00764
MKVLVPVKRVIDYNVKARVKADQTGVDLANVKMSMNPFCEIAVEEAVRLKEKGVATEVVI
VSIGPAQAQETIRTALAMGGDRGVLITTDADPEPLAVAKLLAAVVAEENPNLVLMGKQAI
DGDNNAVGQMLSALLGWPQATYASAIEVSATSAKVTREVDGGLQTVDVDLPAVITADLRL
NEPRYASLPNIMKAKKKEIVSKTVADYGVDIAPRLKVLKVTEPAKRSAGIKVETASDLVS
KLNTAGVL
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory