SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing CCNA_00862 FitnessBrowser__Caulo:CCNA_00862 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 15 hits to proteins with known functional sites (download)

Q9A9Z2 D-xylonate dehydratase; XyDHT; Gluconate dehydratase; EC 4.2.1.82; EC 4.2.1.39 from Caulobacter vibrioides (strain ATCC 19089 / CIP 103742 / CB 15) (Caulobacter crescentus) (see 2 papers)
100% identity, 100% coverage: 1:591/591 of query aligns to 5:595/595 of Q9A9Z2

query
sites
Q9A9Z2

M

L

S

S

N

N

R

R

T

T

P

P

R

R

R

R

F

F

R

R

S

S

R

R

D

D

W

W

F

F

D

D

N

N

P

P

D

D

H

H

I

I

D

D

M

M

T

T

A

A

L

L

Y

Y

L

L

E

E

R

R

F

F

M

M

N

N

Y

Y

G

G

I

I

T

T

P

P

E

E

E

E

L

L

R

R

S

S

G

G

K

K

P

P

I

I

I

I

G

G

I

I

A

A

Q

Q

T

T

G

G

S

S

D

D

I

I

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S

P

P

C
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C

N

N

R

R

I

I

H

H

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L

D

D

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L

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V

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Q

R

R

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V

R

R

D

D

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G

I

I

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R

D

D

A

A

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G

I

I

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M

E

E

F

F

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P

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V

H

H

P

P

I

I

F

F

E
|
E

N

N

C

C

R

R

R

R

P

P

T

T

A

A

A

A

L

L

D

D

R

R

N

N

L

L

S

S

Y

Y

L

L

G

G

L

L

V

V

E

E

T

T

L

L

H

H

G

G

Y

Y

P

P

I

I

D

D

A

A

V

V

V

V

L

L

T

T

T

T

G

G

C
|
C

D
|
D

K

K

T

T

T

T

P

P

A

A

G

G

I

I

M

M

A

A

A

A

T

T

T

T

V

V

N

N

I

I

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P

A

A

I

I

V

V

L

L

S

S

G

G

G

G

P

P

M

M

L

L

D

D

G

G

W

W

H

H

E

E

N

N

E

E

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L

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V

G

G

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S

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G

T

T

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V

I

I

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S

R

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R

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L

A

A

A

A

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E

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E

E

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E

E

E

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F

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I

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D

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R

A

A

A

A

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S

S

A

A

P

P

S

S

A

A

G

G

H

H

C
|
C

N

N

T

T

M

M

G

G

T

T

A

A

S

S

T

T

M

M

N

N

A

A

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V

A

A

E

E

A

A

L

L

G

G

L

L

S

S

L

L

T

T

G

G

C

C

A

A

A

A

I

I

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A

A

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Y

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A

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Y

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A

A

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D

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L

D

D

I

I

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L

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T

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K

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Q

A

A

F

F

E

E

N

N

A

A

I

I

A

A

L

L

V

V

A

A

A

A

A

A

G

G

G

G

S

S

T

T

N

N

A

A

Q

Q

P

P

H

H

I

I

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V

A

A

M

M

A

A

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R

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H

A

A

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E

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T

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D

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A

A

A

A

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Y

D

D

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I

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P

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L

I

I

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V

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A

A

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Y

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A

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H

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D

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V

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L

T

T

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V

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T

G

G

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K

T

T

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E

E

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E

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I

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F

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Y

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H

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E

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P

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A

E

E

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K

A

A

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F

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L

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V

L

L

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K

G

G

N

N

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L

F

F

D

D

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F

A

A

I

I

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M

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K

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I

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N

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D

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P

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A

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L

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E

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D

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E

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R

C
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C

I

I

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L

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V

I

I

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R

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G

A

A

G

G

P

P

I

I

G

G

W

W

P

P

G

G

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S

A

A

E
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E

V

V

V

V

N

N

M

M

Q

Q

P

P

P

P

D

D

H

H

L

L

L

L

K

K

K

K

G

G

I

I

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M

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S

L

L

P

P

T

T

L

L

G

G

D

D

G

G

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R

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Q

S

S

G

G

T

T

A

A

D

D

S

S

P

P

S

S

I

I

L

L

N

N

A

A

S

S

P

P

E

E

S

S

A

A

I

I

G

G

G

G

G

G

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L

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S

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W

L

L

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R

T

T

G

G

D

D

T

T

I

I

R

R

I

I

D

D

L

L

N

N

T

T

G

G

R

R

C

C

D

D

A

A

L

L

V

V

D

D

E

E

A

A

T

T

I

I

A

A

A

A

R

R

K

K

Q

Q

D

D

G

G

I

I

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P

A

A

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V

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P

A

A

T

T

M

M

T

T

P

P

W

W

Q

Q

E

E

I

I

Y

Y

R

R

A

A

H

H

A

A

S

S

Q

Q

L

L

D

D

T

T

G

G

G

G

V

V

L

L

E

E

F

F

A

A

V

V

K

K

Y

Y

Q

Q

D

D

L

L

A

A

A

A

K

K

L

L

P

P

R

R

H

H

N

N

H

H

C64 (= C60) binding [2Fe-2S] cluster; mutation to S: Strong decrease in activity. Does not bind iron-sulfur cluster.
E96 (= E92) binding Mg(2+)
C132 (= C128) binding [2Fe-2S] cluster; mutation to S: Almost loss of activity. Does not bind iron-sulfur cluster.
D133 (= D129) binding Mg(2+)
C205 (= C201) binding [2Fe-2S] cluster; mutation to S: Strong decrease in activity. Does not bind iron-sulfur cluster.
C450 (= C446) mutation to S: Slight decrease in activity. Does not affect binding of iron-sulfur cluster.
E467 (= E463) binding Mg(2+)

5oynA Crystal structure of d-xylonate dehydratase in holo-form (see paper)
100% identity, 100% coverage: 3:591/591 of query aligns to 1:589/589 of 5oynA

query
sites
5oynA

N

N

R

R

T

T

P

P

R

R

R

R

F

F

R

R

S

S

R

R

D

D

W

W

F

F

D

D

N

N

P

P

D

D

H

H

I

I

D

D

M

M

T

T

A

A

L

L

Y

Y

L

L

E

E

R

R

F

F

M

M

N

N

Y

Y

G

G

I

I

T

T

P

P

E

E

E

E

L

L

R

R

S

S

G

G

K

K

P

P

I

I

I

I

G

G

I

I

A

A

Q

Q

T

T

G

G

S

S

D

D

I

I

S

S

P

P

C
|
C

N

N

R

R

I

I

H

H

L

L

D

D

L

L

V

V

Q

Q

R

R

V

V

R

R

D

D

G

G

I

I

R

R

D

D

A

A

G

G

G

G

I

I

P

P

M

M

E

E

F

F

P

P

V

V

H

H

P

P

I

I

F

F

E
|
E

N
|
N

C

C

R

R

R

R

P

P

T

T

A

A

A

A

L

L

D

D

R

R

N

N

L

L

S

S

Y

Y

L

L

G

G

L

L

V

V

E

E

T

T

L

L

H

H

G

G

Y

Y

P

P

I

I

D

D

A

A

V

V

V

V

L

L

T

T

T

T

G

G

C
|
C

D
|
D

K
|
K

T

T

T

T

P

P

A

A

G

G

I

I

M

M

A

A

A

A

T

T

T

T

V

V

N

N

I

I

P

P

A

A

I

I

V

V

L

L

S

S

G

G

G

G

P

P

M

M

L

L

D

D

G

G

W

W

H

H

E

E

N

N

E

E

L

L

V

V

G

G

S

S

G

G

T

T

V

V

I

I

W

W

R

R

S

S

R

R

R

R

K

K

L

L

A

A

A

A

G

G

E

E

I

I

T

T

E

E

E

E

E

E

F

F

I

I

D

D

R

R

A

A

A

A

S

S

S

S

A

A

P

P

S

S

A

A

G

G

H

H

C
|
C

N

N

T

T

M

M

G

G

T

T

A

A

S

S

T

T

M

M

N

N

A

A

V

V

A

A

E

E

A

A

L

L

G

G

L

L

S

S

L

L

T

T

G

G

C

C

A

A

A

A

I

I

P

P

A

A

P

P

Y

Y

R

R

E

E

R

R

G

G

Q

Q

M

M

A

A

Y

Y

K

K

T

T

G

G

Q

Q

R

R

I

I

V

V

D

D

L

L

A

A

Y

Y

D

D

D

D

V

V

K

K

P

P

L

L

D

D

I

I

L

L

T

T

K

K

Q

Q

A

A

F

F

E

E

N

N

A

A

I

I

A

A

L

L

V

V

A

A

A

A

A

A

G

G

G

G

S

S

T

T

N

N

A

A

Q

Q

P

P

H

H

I

I

V

V

A

A

M

M

A

A

R

R

H

H

A

A

G

G

V

V

E

E

I

I

T

T

A

A

D

D

D

D

W

W

R

R

A

A

A

A

Y

Y

D

D

I

I

P

P

L

L

I

I

V

V

N

N

M

M

Q

Q

P

P

A

A

G

G

K

K

Y

Y

L

L

G

G

E

E

R

R

F

F

H

H

R

R

A

A

G

G

G

G

A

A

P

P

A

A

V

V

L

L

W

W

E

E

L

L

L

L

Q

Q

Q

Q

G

G

R

R

L

L

H

H

G

G

D

D

V

V

L

L

T

T

V

V

T

T

G

G

K

K

T

T

M

M

S

S

E

E

N

N

L

L

Q

Q

G

G

R

R

E

E

T

T

S

S

D

D

R

R

E

E

V

V

I

I

F

F

P

P

Y

Y

H

H

E

E

P

P

L

L

A

A

E

E

K

K

A

A

G

G

F

F

L

L

V

V

L

L

K

K

G

G

N

N

L

L

F

F

D

D

F

F

A

A

I

I

M

M

K

K

S

S

S

S

V

V

I

I

G

G

E

E

E

E

F

F

R

R

K

K

R

R

Y

Y

L

L

S

S

Q

Q

P

P

G

G

Q

Q

E

E

G

G

V

V

F

F

E

E

A

A

R

R

A

A

I

I

V

V

F

F

D

D

G

G

S

S

D

D

D

D

Y

Y

H

H

K

K

R

R

I

I

N

N

D

D

P

P

A

A

L

L

E

E

I

I

D

D

E

E

R

R

C

C

I

I

L

L

V

V

I

I

R

R

G

G

A

A

G

G

P

P

I

I

G

G

W

W

P

P

G

G

S

S

A

A

E
|
E

V

V

V

V

N

N

M

M

Q

Q

P

P

P

P

D

D

H

H

L

L

L

L

K

K

K

K

G

G

I

I

M

M

S

S

L

L

P

P

T

T

L

L

G

G

D

D

G

G

R

R

Q

Q

S

S

G

G

T

T

A

A

D

D

S

S

P

P

S

S

I

I

L

L

N

N

A

A

S

S

P

P

E

E

S

S

A

A

I

I

G

G

G

G

G

G

L

L

S

S

W

W

L

L

R

R

T

T

G

G

D

D

T

T

I

I

R

R

I

I

D

D

L

L

N

N

T

T

G

G

R

R

C

C

D

D

A

A

L

L

V

V

D

D

E

E

A

A

T

T

I

I

A

A

A

A

R

R

K

K

Q

Q

D

D

G

G

I

I

P

P

A

A

V

V

P

P

A

A

T

T

M

M

T

T

P

P

W

W

Q

Q

E

E

I

I

Y

Y

R

R

A

A

H

H

A

A

S

S

Q

Q

L

L

D

D

T

T

G

G

G

G

V

V

L

L

E

E

F

F

A

A

V

V

K

K

Y

Y

Q

Q

D

D

L

L

A

A

A

A

K

K

L

L

P

P

R

R

H

H

N

N

H

H

binding fe2/s2 (inorganic) cluster: C58 (= C60), N91 (= N93), C126 (= C128), D127 (= D129), C199 (= C201)
binding magnesium ion: E90 (= E92), D127 (= D129), K128 (= K130), E461 (= E463)

7m3kA Crystal structure of galactonate dehydratase from brucella melitensis biovar abortus 2308
63% identity, 97% coverage: 8:583/591 of query aligns to 1:581/587 of 7m3kA

query
sites
7m3kA

R

K

F

L

R

R

S

S

R

R

D

A

W

W

F

F

D

D

N

N

P

P

D

D

H

D

I

V

D

D

M

M

T

T

A

A

L

L

Y

Y

L

L

E

E

R

R

F

Y

M

M

N

N

Y

Y

G

G

I

L

T

S

P

Q

E

E

E

E

L

L

R

Q

S

S

G

G

K

R

P

P

I

I

I

I

G

G

I

I

A

A

Q

Q

T

T

G

G

S

S

D

D

I

L

S

S

P

P

C
|
C

N

N

R

R

I

H

H

H

L

L

D

E

L

L

V

A

Q

K

R

R

V

V

R

R

D

D

G

G

I

V

R

R

D

E

A

A

G

G

G

G

I

I

P

V

M

I

E

E

F

F

P

P

V

V

H

H

P

P

I

I

F

Q

E

E

N

T

C

G

R

K

R

R

P

P

T

T

A

A

A

G

L

L

D

D

R

R

N

N

L

L

S

A

Y

Y

L

L

G

G

L

L

V

V

E

E

T

V

L

L

H

Y

G

G

Y

Y

P

P

I

L

D

D

A

G

V

V

V

V

L

L

T

T

T

I

G

G

C
|
C

D

D

K

K

T

T

T

T

P

P

A

A

G

C

I

L

M

M

A

A

A

A

T

A

T

T

V

V

N

N

I

I

P

P

A

A

I

I

V

A

L

L

S

S

G

V

G

G

P

P

M

M

L

L

D

N

G

G

W

W

H

F

E

R

N

G

E

E

L

R

V

T

G

G

S

S

G

G

T

T

V

I

I

V

W

W

R

K

S

A

R

R

R

E

K

L

L

L

A

A

A

K

G

G

E

E

I

I

T

D

E

Y

E

Q

E

G

F

F

I

V

D

K

R

L

A

V

A

A

S

S

S

S

A

A

P

P

S

S

A

T

G

G

H

Y

C

C

N

N

T

T

M

M

G

G

T

T

A

A

S

T

T

T

M

M

N

N

A

S

V

L

A

A

E

E

A

A

L

L

G

G

L

M

S

Q

L

L

T

P

G

G

C

S

A

A

A

A

I

I

P

P

A

A

P

P

Y

Y

R

R

E

D

R

R

G

Q

Q

E

M

V

A

A

Y

Y

K

L

T

M

G

G

Q

R

R

R

I

I

V

V

D

E

L

M

A

V

Y

H

D

E

D

D

V

L

K

K

P

P

L

S

D

D

I

I

L

L

T

T

K

K

Q

E

A

A

F

F

E

I

N

N

A

A

I

I

A

R

L

V

V

N

A

S

A

A

A

I

G

G

G

G

S

S

T

T

N

N

A

A

Q

P

P

I

H

H

I

L

V

N

A

A

M

L

A

A

R

R

H

H

A

I

G

G

V

V

E

E

I

L

T

T

A

V

D

D

D

D

W

W

-

Q

R

K

A

Y

A

G

Y

E

D

E

I

I

P

P

L

L

I

L

V

V

N

N

M

L

Q

Q

P

P

A

A

G

G

K

E

Y

Y

L

L

G

G

E

E

R

D

F

Y

H

Y

R

H

A

A

G

G

G

G

A

V

P

P

A

A

V

V

L

V

W

N

E

Q

L

L

L

M

Q

G

Q

Q

G

G

R

L

L

I

H

H

G

E

D

D

V

A

L

I

T

T

V

V

T

N

G

G

K

K

T

T

M

I

S

G

E

E

N

N

L

C

Q

K

G

N

R

A

E

T

T

I

S

E

D

D

R

G

E

N

V

V

I

I

F

K

P

T

Y

Y

H

D

E

Q

P

P

L

L

A

K

E

K

K

H

A

A

G

G

F

F

L

R

V

V

L

L

K

R

G

G

N

N

L

L

F

F

D

S

F

S

A

A

I

I

M

M

K

K

S

L

S

S

V

V

I

I

G

S

E

D

E

E

F

F

R

R

K

N

R

R

Y

Y

L

L

S

S

Q

D

P

A

G

K

Q

D

E

P

G

N

V

A

F

F

E

E

A

G

R

K

A

A

I

V

V

V

F

F

D

D

G

G

S

P

D

E

D

D

Y

Y

H

H

K

H

R

R

I

I

N

D

D

D

P

P

A

A

L

L

E

E

I

I

D

D

E

E

R

H

C

T

I

V

L

L

V

F

I

M

R

R

G

G

A

A

G

G

P

P

I

I

G

G

W

Y

P

P

G

G

S

A

A

A

E

E

V

V

V

V

N

N

M

M

Q

R

P

A

P

P

D

D

H

Y

L

L

L

L

K

K

K

K

G

G

I

I

M

T

S

S

L

L

P

P

T

C

L

I

G

G

D

D

G

G

R

R

Q

Q

S

S

G

G

T

T

A

S

D

G

S

S

P

P

S

S

I

I

L

L

N

N

A

A

S

S

P

P

E

E

S

A

A

A

I

A

G

G

G

G

G

G

L

L

S

A

W

I

L

L

R

K

T

T

G

G

D

D

T

R

I

V

R

R

I

I

D

D

L

L

N

G

T

R

G

G

R

T

C

A

D

D

A

I

L

L

V

I

D

S

E

D

A

E

T

E

I

L

A

A

A

E

R

R

K

R

Q

K

D

-

G

A

I

L

P

E

A

A

V

V

-

G

-

G

-

Y

-

K

-

Y

P

P

A

E

T

S

M

Q

T

T

P

P

W

W

Q

Q

E

E

I

I

Y

Q

R

R

A

A

H

V

A

I

S

G

Q

Q

L

M

D

E

T

T

G

G

G

A

V

V

L

L

E

E

F

N

A

A

V

V

K

K

Y

Y

Q

Q

D

D

L

I

A

A

binding zinc ion: C53 (= C60), C121 (= C128)

9evvD His579leu variant of l-arabinonate dehydratase co-crystallized with 2- oxobutyrate (see paper)
42% identity, 96% coverage: 6:575/591 of query aligns to 4:561/575 of 9evvD

query
sites
9evvD

P

P

R

R

R

K

F

L

R

R

S

S

R

Q

D

E

W

W

F

Y

D

G

N

G

P

T

D

S

H

R

I

-

D

-

M

-

T

D

A

V

L

I

Y

Y

L

H

E

R

R

G

F

W

M

L

N

K

Y

N

G

Q

I

G

T

Y

P

P

E

H

E

D

L

L

R

F

S

D

G

G

K

R

P

P

I

V

I

I

G

G

I

I

A

L

Q

N

T

T

G

W

S

S

D

D

I

M

S

T

P

P

C
|
C

N

N

R

G

I

H

H

L

L

R

D

E

L

L

V

A

Q

E

R

K

V

V

R

K

D

A

G

G

I

V

R

W

D

E

A

A

G

G

G

G

I

F

P

P

M

L

E

E

F

V

P

P

V

V

H

F

P

S

I

A

F

S

E
|
E

N

N

C

T

R

F

R

R

P

P

T

T

A

A

A

M

L

M

D

Y

R

R

N

N

L

L

S

A

Y

A

L

L

G

A

L

V

V

E

E

E

T

A

L

I

H

R

G

G

Y

Q

P

P

I

M

D

D

A

G

V

C

V

V

L

L

T

L

T

V

G

G

C
|
C

D
|
D

K
|
K

T

T

T

T

P

P

A

S

G

L

I

L

M

M

A

G

A

A

T

A

T

S

V

C

N

D

I

L

P

P

A

S

I

I

V

V

L

V

S

T

G

G

G

G

P

P

M

M

L

L

D

N

G

G

W

Y

H

F

E

R

N

G

E

E

L

R

V

V

G

G

S

S

G

G

T

T

V

H

I

L

W

W

R

K

S

F

R

S

R

E

K

M

L

V

A

K

A

A

G

G

E

E

I

M

T

T

E

Q

E

A

E

E

F

F

I

L

D

E

R

A

A

E

A

A

S

S

S

M

A

S

P

R

S

S

A

S

G

G

H
x
T

C
|
C

N

N

T

T

M

M

G

G

T
|
T

A

A

S

S

T

T

M

M

N

A

A

S

V

M

A

A

E

E

A

A

L

L

G

G

L

M

S

A

L

L

T

S

G

G

C

N

A

A

A

A

I

I

P

P

A

G

P

V

Y

D

R

S

E

R

R

R

G

K

Q

V

M

M

A

A

Y

Q

K

L

T

T

G

G

Q

R

R

R

I

I

V

V

D

Q

L

M

A

V

Y

K

D

D

D

D

V

L

K

K

P

P

L

S

D

E

I

I

L

M

T

T

K

K

Q

Q

A

A

F

F

E

E

N

N

A

A

I

I

A

R

L

T

V

N

A

A

A

A

A

I

G

G

G

G

S

S

T

T

N

N

A

A

Q

V

P

I

H

H

I

L

V

L

A

A

M

I

A

A

R

G

H

R

A

V

G

G

V

I

E

D

I

L

T

S

A

L

D

D

D

D

W

W

-

D

R

R

A

C

A

G

Y

R

D

D

I

V

P

P

L

T

I

I

V

V

N

N

M

L

Q

M

P

P

A

S

G

G

K

K

Y

Y

L

L

G

M

E

E

R

E

F

F

H

F

R

Y

A

A

G

G

G

G

A

L

P

P

A

V

V

V

L

L

W

K

E

R

L

L

L

G

Q

E

Q

A

G

G

R

L

L

L

H

H

G

K

D

D

V

A

L

L

T

T

V

V

T

S

G

G

K

E

T

T

M

V

S

W

E

D

N

E

L

V

Q

K

G

D

R

V

E

V

T

N

S

W

D

N

R

E

E

D

V

V

I

I

F

L

P

P

Y

A

H

E

E

K

P

A

L

L

A

T

E

S

K

S

A

G

G

G

F

I

L

V

V

V

L

L

K

R

G

G

N

N

L

L

F

A

-

P

D

K

F

G

A

A

I

V

M

L

K

K

S

P

S

S

V

A

I

A

G

S

E

P

E

H

F

L

R

L

K

-

R

-

Y

-

L

-

S

-

Q

-

P

-

G

-

Q

-

E

-

G

-

V

V

F

H

E

K

A

G

R

R

A

A

I

V

V

V

F

F

D

E

G

D

S

I

D

D

D

D

Y

Y

H

K

K

A

R

K

I

I

N

N

D

D

P

D

A

N

L

L

E

D

I

I

D

D

E

E

R

N

C

C

I

I

L

M

V

V

I

M

R

K

G

N

A

C

G

G

P

P

I

K

G

G

W

Y

P

P

G

G

S

M

A

A

E
|
E

V

V

V

G

N

N

M

M

Q

G

P

L

P

P

D

P

H

K

L

V

L

L

K

K

K

K

G

G

I

I

M

L

S

D

L

M

P

V

T

R

L

I

G

S

D

D

G

A

R

R

Q

M

S
|
S

G
|
G

T

T

A

A

D

Y

S

G

P

T

S

V

I

V

L

L

N

H

A

T

S

S

P

P

E

E

S

A

A

A

I

V

G

G

G

G

G

P

L

L

S

A

W

V

L

V

R

K

T

N

G

G

D

D

T

M

I

I

R

E

I

L

D

D

L

V

N

P

T

N

G

R

R

R

C

L

D

H

A

L

L

D

V

I

D

S

E

D

A

E

T

E

I

L

A

A

A

R

R

R

K

L

Q

A

D

E

G

W

I

Q

P

P

A

N

V

H

P

D

A

L

T

P

M

T

T

S

P

G

W

Y

Q

A

E

F

I

L

Y

H

R

Q

A

Q

H

H

A

V

S

E

Q

G

L

A

D

D

T

T

G

G

G

A

V

D

L

L

E

D

F

F

binding 2-ketobutyric acid: E87 (= E92), D124 (= D129), T201 (= T206), E449 (= E463), S476 (= S490), G477 (= G491)
binding fe2/s2 (inorganic) cluster: C55 (= C60), C123 (= C128), D124 (= D129), T195 (≠ H200), C196 (= C201)
binding magnesium ion: E87 (= E92), D124 (= D129), K125 (= K130), E449 (= E463)

9evvA His579leu variant of l-arabinonate dehydratase co-crystallized with 2- oxobutyrate (see paper)
42% identity, 96% coverage: 6:575/591 of query aligns to 4:561/575 of 9evvA

query
sites
9evvA

P

P

R

R

R

K

F

L

R

R

S

S

R

Q

D

E

W

W

F

Y

D

G

N

G

P

T

D

S

H

R

I

-

D

-

M

-

T

D

A

V

L

I

Y

Y

L

H

E

R

R

G

F

W

M

L

N

K

Y

N

G

Q

I

G

T

Y

P

P

E

H

E

D

L

L

R

F

S

D

G

G

K

R

P

P

I

V

I

I

G

G

I

I

A

L

Q

N

T

T

G

W

S

S

D

D

I

M

S

T

P

P

C
|
C

N

N

R

G

I

H

H

L

L

R

D

E

L

L

V

A

Q

E

R

K

V

V

R

K

D

A

G

G

I

V

R

W

D

E

A

A

G

G

G

G

I

F

P

P

M

L

E

E

F

V

P

P

V

V

H

F

P

S

I

A

F

S

E
|
E

N
|
N

C

T

R

F

R

R

P

P

T

T

A

A

A

M

L

M

D

Y

R

R

N

N

L

L

S

A

Y

A

L

L

G

A

L

V

V

E

E

E

T

A

L

I

H

R

G

G

Y

Q

P

P

I

M

D

D

A

G

V

C

V

V

L

L

T

L

T

V

G

G

C
|
C

D
|
D

K
|
K

T

T

T

T

P

P

A

S

G

L

I

L

M

M

A

G

A

A

T

A

T

S

V

C

N

D

I

L

P

P

A

S

I

I

V

V

L

V

S

T

G

G

G

G

P

P

M

M

L

L

D

N

G

G

W

Y

H

F

E

R

N

G

E

E

L

R

V

V

G

G

S

S

G

G

T

T

V

H

I

L

W

W

R

K

S

F

R

S

R

E

K

M

L

V

A

K

A

A

G

G

E

E

I

M

T

T

E

Q

E

A

E

E

F

F

I

L

D

E

R

A

A

E

A

A

S

S

S

M

A

S

P

R

S

S

A

S

G

G

H
x
T

C
|
C

N

N

T

T

M

M

G

G

T

T

A

A

S

S

T

T

M

M

N

A

A

S

V

M

A

A

E

E

A

A

L

L

G

G

L

M

S

A

L

L

T

S

G

G

C

N

A

A

A

A

I

I

P

P

A

G

P

V

Y

D

R

S

E

R

R

R

G

K

Q

V

M

M

A

A

Y

Q

K

L

T

T

G

G

Q

R

R

R

I

I

V

V

D

Q

L

M

A

V

Y

K

D

D

D

D

V

L

K

K

P

P

L

S

D

E

I

I

L

M

T

T

K

K

Q

Q

A

A

F

F

E

E

N

N

A

A

I

I

A

R

L

T

V

N

A

A

A

A

A

I

G

G

G

G

S

S

T

T

N

N

A

A

Q

V

P

I

H

H

I

L

V

L

A

A

M

I

A

A

R

G

H

R

A

V

G

G

V

I

E

D

I

L

T

S

A

L

D

D

D

D

W

W

-

D

R

R

A

C

A

G

Y

R

D

D

I

V

P

P

L

T

I

I

V

V

N

N

M

L

Q

M

P

P

A

S

G

G

K

K

Y

Y

L

L

G

M

E

E

R

E

F

F

H

F

R

Y

A

A

G

G

G

G

A

L

P

P

A

V

V

V

L

L

W

K

E

R

L

L

L

G

Q

E

Q

A

G

G

R

L

L

L

H

H

G

K

D

D

V

A

L

L

T

T

V

V

T

S

G

G

K

E

T

T

M

V

S

W

E

D

N

E

L

V

Q

K

G

D

R

V

E

V

T

N

S

W

D

N

R

E

E

D

V

V

I

I

F

L

P

P

Y

A

H

E

E

K

P

A

L

L

A

T

E

S

K

S

A

G

G

G

F

I

L

V

V

V

L

L

K

R

G

G

N

N

L

L

F

A

-

P

D

K

F

G

A

A

I

V

M

L

K

K

S

P

S

S

V

A

I

A

G

S

E

P

E

H

F

L

R

L

K

-

R

-

Y

-

L

-

S

-

Q

-

P

-

G

-

Q

-

E

-

G

-

V

V

F

H

E

K

A

G

R

R

A

A

I

V

V

V

F

F

D

E

G

D

S

I

D

D

D

D

Y

Y

H

K

K

A

R

K

I

I

N

N

D

D

P

D

A

N

L

L

E

D

I

I

D

D

E

E

R

N

C

C

I

I

L

M

V

V

I

M

R

K

G

N

A

C

G

G

P

P

I

K

G

G

W

Y

P

P

G

G

S

M

A

A

E
|
E

V

V

V

G

N

N

M

M

Q

G

P

L

P

P

D

P

H

K

L

V

L

L

K

K

K

K

G

G

I

I

M

L

S

D

L

M

P

V

T

R

L

I

G

S

D

D

G

A

R

R

Q

M

S

S

G

G

T

T

A

A

D

Y

S

G

P

T

S

V

I

V

L

L

N

H

A

T

S

S

P

P

E

E

S

A

A

A

I

V

G

G

G

G

G

P

L

L

S

A

W

V

L

V

R

K

T

N

G

G

D

D

T

M

I

I

R

E

I

L

D

D

L

V

N

P

T

N

G

R

R

R

C

L

D

H

A

L

L

D

V

I

D

S

E

D

A

E

T

E

I

L

A

A

A

R

R

R

K

L

Q

A

D

E

G

W

I

Q

P

P

A

N

V

H

P

D

A

L

T

P

M

T

T

S

P

G

W

Y

Q

A

E

F

I

L

Y

H

R

Q

A

Q

H

H

A

V

S

E

Q

G

L

A

D

D

T

T

G

G

G

A

V

D

L

L

E

D

F

F

binding fe2/s2 (inorganic) cluster: C55 (= C60), N88 (= N93), C123 (= C128), D124 (= D129), T195 (≠ H200), C196 (= C201)
binding magnesium ion: E87 (= E92), D124 (= D129), K125 (= K130), E449 (= E463)

B5ZZ34 L-arabinonate dehydratase; ArDHT; D-fuconate dehydratase; Galactonate dehydratase; L-arabonate dehydratase; EC 4.2.1.25; EC 4.2.1.67; EC 4.2.1.6 from Rhizobium leguminosarum bv. trifolii (strain WSM2304) (see 2 papers)
42% identity, 96% coverage: 6:575/591 of query aligns to 8:565/579 of B5ZZ34

query
sites
B5ZZ34

P

P

R

R

R

K

F

L

R

R

S

S

R

Q

D

E

W

W

F

Y

D

G

N

G

P

T

D

S

H

R

I

-

D

-

M

-

T

D

A

V

L

I

Y

Y

L

H

E

R

R

G

F

W

M

L

N

K

Y

N

G

Q

I

G

T

Y

P

P

E

H

E

D

L

L

R

F

S

D

G

G

K

R

P

P

I

V

I

I

G

G

I

I

A

L

Q

N

T

T

G

W

S

S

D

D

I

M

S

T

P

P

C
|
C

N

N

R

G

I

H

H

L

L

R

D

E

L

L

V

A

Q

E

R

K

V

V

R

K

D

A

G

G

I

V

R

W

D

E

A

A

G

G

G

G

I

F

P

P

M

L

E

E

F

V

P

P

V

V

H

F

P

S

I

A

F

S

E
|
E

N

N

C

T

R

F

R

R

P

P

T

T

A

A

A

M

L

M

D

Y

R

R

N

N

L

L

S

A

Y

A

L

L

G

A

L

V

V

E

E

E

T

A

L

I

H

R

G

G

Y

Q

P

P

I

M

D

D

A

G

V

C

V

V

L

L

T

L

T

V

G

G

C
|
C

D
|
D

K

K

T

T

T

T

P

P

A

S

G

L

I

L

M

M

A

G

A

A

T

A

T

S

V

C

N

D

I

L

P

P

A

S

I

I

V

V

L

V

S

T

G

G

G

G

P

P

M

M

L

L

D

N

G

G

W

Y

H

F

E

R

N

G

E

E

L

R

V

V

G

G

S

S

G

G

T

T

V

H

I

L

W

W

R

K

S

F

R

S

R

E

K

M

L

V

A

K

A

A

G

G

E

E

I

M

T

T

E

Q

E

A

E

E

F

F

I

L

D

E

R

A

A

E

A

A

S

S

S

M

A

S

P

R

S

S

A

S

G

G

H

T

C
|
C

N

N

T

T

M

M

G

G

T

T

A

A

S

S

T

T

M

M

N

A

A

S

V

M

A

A

E

E

A

A

L

L

G

G

L

M

S

A

L

L

T

S

G

G

C

N

A

A

A

A

I

I

P

P

A

G

P

V

Y

D

R

S

E

R

R

R

G

K

Q

V

M

M

A

A

Y

Q

K

L

T

T

G

G

Q

R

R

R

I

I

V

V

D

Q

L

M

A

V

Y

K

D

D

D

D

V

L

K

K

P

P

L

S

D

E

I

I

L

M

T

T

K

K

Q

Q

A

A

F

F

E

E

N

N

A

A

I

I

A

R

L

T

V

N

A

A

A

A

A

I

G

G

G

G

S

S

T

T

N

N

A

A

Q

V

P

I

H

H

I

L

V

L

A

A

M

I

A

A

R

G

H

R

A

V

G

G

V

I

E

D

I

L

T

S

A

L

D

D

D

D

W

W

-

D

R

R

A

C

A

G

Y

R

D

D

I

V

P

P

L

T

I

I

V

V

N

N

M

L

Q

M

P

P

A

S

G

G

K

K

Y

Y

L

L

G

M

E

E

R

E

F

F

H

F

R

Y

A

A

G

G

G

G

A

L

P

P

A

V

V

V

L

L

W

K

E

R

L

L

L

G

Q

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Q

A

G

G

R

L

L

L

H

H

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K

D

D

V

A

L

L

T

T

V

V

T

S

G

G

K

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T

T

M

V

S

W

E

D

N

E

L

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Q

K

G

D

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V

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V

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N

S

W

D

N

R

E

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D

V

V

I

I

F

L

P

P

Y

A

H

E

E

K

P

A

L

L

A

T

E

S

K

S

A

G

G

G

F

I

L

V

V

V

L

L

K

R

G

G

N

N

L

L

F

A

-

P

D

K

F

G

A

A

I

V

M

L

K

K

S

P

S

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V

A

I

A

G

S

E

P

E

H

F

L

R

L

K

-

R

-

Y

-

L

-

S

-

Q

-

P

-

G

-

Q

-

E

-

G

-

V

V

F

H

E

K

A

G

R

R

A

A

I

V

V

V

F

F

D

E

G

D

S

I

D

D

D

D

Y

Y

H

K

K

A

R

K

I

I

N

N

D

D

P

D

A

N

L

L

E

D

I

I

D

D

E

E

R

N

C
|
C

I

I

L

M

V

V

I

M

R

K

G

N

A
x
C

G

G

P

P

I

K

G

G

W

Y

P

P

G

G

S

M

A

A

E
|
E

V

V

V

G

N

N

M

M

Q

G

P

L

P

P

D

P

H

K

L

V

L

L

K

K

K

K

G

G

I

I

M

L

S

D

L

M

P

V

T

R

L

I

G

S

D

D

G

A

R

R

Q

M

S
|
S

G

G

T

T

A

A

D

Y

S

G

P

T

S

V

I

V

L

L

N

H

A

T

S

S

P

P

E

E

S

A

A

A

I

V

G

G

G

G

G

P

L

L

S

A

W

V

L

V

R

K

T

N

G

G

D

D

T

M

I

I

R

E

I

L

D

D

L

V

N

P

T

N

G

R

R

R

C

L

D

H

A

L

L

D

V

I

D

S

E

D

A

E

T

E

I

L

A

A

A

R

R

R

K

L

Q

A

D

E

G

W

I

Q

P

P

A

N

V

H

P

D

A

L

T

P

M

T

T

S

P

G

W

Y

Q

A

E

F

I

L

Y

H

R

Q

A

Q

H

H

A

V

S

E

Q

G

L

A

D

D

T

T

G

G

G

A

V

D

L

L

E

D

F

F

C59 (= C60) binding [2Fe-2S] cluster; mutation to S: Loss of activity. Does not bind iron-sulfur cluster.
E91 (= E92) binding Mg(2+)
C127 (= C128) binding [2Fe-2S] cluster; mutation to S: Loss of activity. Does not bind iron-sulfur cluster.
D128 (= D129) binding Mg(2+)
C200 (= C201) binding [2Fe-2S] cluster; mutation to S: Almost loss of activity. Does not bind iron-sulfur cluster.
C436 (= C446) mutation to S: No change in activity. Does not affect binding of iron-sulfur cluster.
C443 (≠ A453) mutation to S: Slight decrease in activity. Does not affect binding of iron-sulfur cluster.
E453 (= E463) binding Mg(2+)
S480 (= S490) mutation to A: Loss of activity.

5j85A Ser480ala mutant of l-arabinonate dehydratase (see paper)
42% identity, 96% coverage: 6:575/591 of query aligns to 5:562/576 of 5j85A

query
sites
5j85A

P

P

R

R

R

K

F

L

R

R

S

S

R

Q

D

E

W

W

F

Y

D

G

N

G

P

T

D

S

H

R

I

-

D

-

M

-

T

D

A

V

L

I

Y

Y

L

H

E

R

R

G

F

W

M

L

N

K

Y

N

G

Q

I

G

T

Y

P

P

E

H

E

D

L

L

R

F

S

D

G

G

K

R

P

P

I

V

I

I

G

G

I

I

A

L

Q

N

T

T

G

W

S

S

D

D

I

M

S

T

P

P

C
|
C

N

N

R

G

I

H

H

L

L

R

D

E

L

L

V

A

Q

E

R

K

V

V

R

K

D

A

G

G

I

V

R

W

D

E

A

A

G

G

G

G

I

F

P

P

M

L

E

E

F

V

P

P

V

V

H

F

P

S

I

A

F

S

E
|
E

N
|
N

C

T

R

F

R

R

P

P

T

T

A

A

A

M

L

M

D

Y

R

R

N

N

L

L

S

A

Y

A

L

L

G

A

L

V

V

E

E

E

T

A

L

I

H

R

G

G

Y

Q

P

P

I

M

D

D

A

G

V

C

V

V

L

L

T

L

T

V

G

G

C
|
C

D
|
D

K
|
K

T

T

T

T

P

P

A

S

G

L

I

L

M

M

A

G

A

A

T

A

T

S

V

C

N

D

I

L

P

P

A

S

I

I

V

V

L

V

S

T

G

G

G

G

P

P

M

M

L

L

D

N

G

G

W

Y

H

F

E

R

N

G

E

E

L

R

V

V

G

G

S

S

G

G

T

T

V

H

I

L

W

W

R

K

S

F

R

S

R

E

K

M

L

V

A

K

A

A

G

G

E

E

I

M

T

T

E

Q

E

A

E

E

F

F

I

L

D

E

R

A

A

E

A

A

S

S

S

M

A

S

P

R

S

S

A

S

G

G

H
x
T

C
|
C

N

N

T

T

M

M

G

G

T
|
T

A

A

S

S

T

T

M

M

N

A

A

S

V

M

A

A

E

E

A

A

L

L

G

G

L

M

S

A

L

L

T

S

G

G

C

N

A

A

A

A

I

I

P

P

A

G

P

V

Y

D

R

S

E

R

R

R

G

K

Q

V

M

M

A

A

Y

Q

K

L

T

T

G

G

Q

R

R

R

I

I

V

V

D

Q

L

M

A

V

Y

K

D

D

D

D

V

L

K

K

P

P

L

S

D

E

I

I

L

M

T

T

K

K

Q

Q

A

A

F

F

E

E

N

N

A

A

I

I

A

R

L

T

V

N

A

A

A

A

A

I

G

G

G

G

S

S

T

T

N

N

A

A

Q

V

P

I

H

H

I

L

V

L

A

A

M

I

A

A

R

G

H

R

A

V

G

G

V

I

E

D

I

L

T

S

A

L

D

D

D

D

W

W

-

D

R

R

A

C

A

G

Y

R

D

D

I

V

P

P

L

T

I

I

V

V

N

N

M

L

Q

M

P

P

A

S

G

G

K

K

Y

Y

L

L

G

M

E

E

R

E

F

F

H

F

R

Y

A

A

G

G

G

G

A

L

P

P

A

V

V

V

L

L

W

K

E

R

L

L

L

G

Q

E

Q

A

G

G

R

L

L

L

H

H

G

K

D

D

V

A

L

L

T

T

V

V

T

S

G

G

K

E

T

T

M

V

S

W

E

D

N

E

L

V

Q

K

G

D

R

V

E

V

T

N

S

W

D

N

R

E

E

D

V

V

I

I

F

L

P

P

Y

A

H

E

E

K

P

A

L

L

A

T

E

S

K

S

A

G

G

G

F

I

L

V

V

V

L

L

K

R

G

G

N

N

L

L

F

A

-

P

D

K

F

G

A

A

I

V

M

L

K

K

S

P

S

S

V

A

I

A

G

S

E

P

E

H

F

L

R

L

K

-

R

-

Y

-

L

-

S

-

Q

-

P

-

G

-

Q

-

E

-

G

-

V

V

F

H

E

K

A

G

R

R

A

A

I

V

V

V

F

F

D

E

G

D

S

I

D

D

D

D

Y

Y

H

K

K

A

R

K

I

I

N

N

D

D

P

D

A

N

L

L

E

D

I

I

D

D

E

E

R

N

C

C

I

I

L

M

V

V

I

M

R

K

G

N

A

C

G

G

P

P

I

K

G

G

W

Y

P

P

G

G

S

M

A

A

E
|
E

V

V

V

G

N

N

M

M

Q

G

P

L

P

P

D

P

H

K

L

V

L

L

K

K

K

K

G

G

I

I

M

L

S

D

L

M

P

V

T

R

L

I

G

S

D

D

G

A

R

R

Q

M

S

A

G

G

T

T

A

A

D

Y

S

G

P

T

S

V

I

V

L

L

N

H

A

T

S

S

P

P

E

E

S

A

A

A

I

V

G

G

G

G

G

P

L

L

S

A

W

V

L

V

R

K

T

N

G

G

D

D

T

M

I

I

R

E

I

L

D

D

L

V

N

P

T

N

G

R

R

R

C

L

D

H

A

L

L

D

V

I

D

S

E

D

A

E

T

E

I

L

A

A

A

R

R

R

K

L

Q

A

D

E

G

W

I

Q

P

P

A

N

V

H

P

D

A

L

T

P

M

T

T

S

P

G

W

Y

Q

A

E

F

I

L

Y

H

R

Q

A

Q

H

H

A

V

S

E

Q

G

L

A

D

D

T

T

G

G

G

A

V

D

L

L

E

D

F

F

binding fe2/s2 (inorganic) cluster: C56 (= C60), N89 (= N93), C124 (= C128), D125 (= D129), T196 (≠ H200), C197 (= C201)
binding magnesium ion: E88 (= E92), D125 (= D129), K126 (= K130), T202 (= T206), E450 (= E463)

8ej0A Crystal structure of fe-s cluster-dependent dehydratase from paralcaligenes ureilyticus in complex with mg (see paper)
41% identity, 98% coverage: 10:591/591 of query aligns to 3:568/568 of 8ej0A

query
sites
8ej0A

R

R

S

S

R

Q

D

A

W

W

F

F

D

G

N

R

P

L

D

D

H

R

I

D

D

G

M

F

T

-

A

-

L

I

Y

Y

L

R

E

S

R

W

F

M

M

K

N

N

Y

R

G

G

I

I

T

-

P

P

E

H

E

D

L

Q

R

F

S

D

G

G

K

R

P

P

I

V

I

I

G

G

I

I

A

C

Q

N

T

T

G

F

S

S

D

E

I

L

S

T

P

P

C
|
C

N

N

R

S

I

H

H

F

L

R

D

T

L

L

V

A

Q

E

R

Q

V

V

R

K

D

I

G

G

I

V

R

W

D

E

A

S

G

G

G

G

I

F

P

P

M

L

E

E

F

F

P

P

V

V

H

M

P

S

I

L

F

G

E
|
E

N

T

C

M

R

L

R

R

P

P

T

T

A

A

A

M

L

L

D

F

R

R

N

N

L

L

S

A

Y

S

L

M

G

D

L

V

V

E

E

E

T

S

L

I

H

R

G

G

Y

N

P

P

I

L

D

D

A

G

V

V

V

V

L

L

T

L

T

M

G

G

C
|
C

D
|
D

K
|
K

T

T

T

T

P

P

A

S

G

L

I

M

M

M

A

G

A

A

T

A

T

S

V

C

N

D

I

L

P

P

A

T

I

I

V

G

L

V

S

S

G

G

G

G

P

P

M

M

L

L

D

S

G

G

W

K

H

F

E

R

N

G

E

R

L

E

V

L

G

G

S

S

G

G

T

T

V

D

I

V

W
|
W

R

K

S

M

R

S

R

E

K

E

L

V

A

R

A

A

G

G

E

Q

I

M

T

S

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Q

E

E

E

E

F

F

I

F

D

E

R

A

A

E

A

S

S

C

S

M

A

H

P

R

S

S

A

H

G

G

H

H

C
|
C

N

M

T

T

M

M

G

G

T
|
T

A

A

S

S

T

T

M

M

N

A

A

S

V

M

A

V

E

E

A

A

L

L

G

G

L

M

S

S

L

L

T

P

G

G

C

N

A

A

A

A

I

I

P

P

A

A

P

V

Y

D

R

A

E

R

R

R

G

N

Q

L

M

L

A

A

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K

A

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G

G

Q

R

R

R

I

I

V

V

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Q

L

M

A

V

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K

D

D

D

D

V

L

K

V

P

M

L

S

D

K

I

I

L

L

T

T

K

R

Q

Q

A

A

F

F

E

E

N

N

A

A

I

I

A

R

L

V

V

N

A

A

A

A

A

I

G

G

G

G

S

S

T

T

N

N

A

A

Q

V

P

I

H

H

I

L

V

L

A

A

M

I

A

A

R

G

H

R

A

I

G

G

V

V

E

D

I

L

T

T

A

L

D

A

D

D

W

W

R

D

A

A

-

L

A

G

Y

H

D

K

I

L

P

P

L

C

I

L

V

V

N

D

M

L

Q

Q

P

P

A

S

G

G

K

T

Y

H

L

L

G

M

E

E

R

D

F

F

H

Y

R

Y

A

A

G

G

G

G

A

V

P

P

A

A

V

V

L

I

W

R

E

E

L

L

L

-

Q

-

Q

G

G

D

R

V

L

I

H

A

G

R

D

D

V

A

L

L

T

T

V

V

T

N

G

G

K

Q

T

T

M

L

S

W

E

D

N

N

L

C

Q

K

G

D

R

A

E

P

T

N

S

W

D

N

R

R

E

E

V

V

I

I

F

H

P

A

Y

F

H

N

E

E

P

P

L

F

A

K

E

T

K

E

A

A

G

G

F

I

L

A

V

V

L

L

K

R

G

G

N

N

L

L

F

C

-

P

D

D

F

G

A

A

I

V

M

I

K

K

S

P

S

S

V

A

I

A

G

T

E

P

E

A

F

L

R

L

K

K

R

H

Y

-

L

-

S

-

Q

-

P

-

G

-

Q

-

E

-

G

-

V

-

F

-

E

K

A

G

R

R

A

A

I

V

V

V

F

F

D

E

G

N

S

S

D

E

D

H

Y

M

H

H

K

E

R

R

I

M

N

D

D

D

P

E

A

N

L

L

E

D

I

V

D

D

E

E

R

N

C

C

I

V

L

L

V

V

I

L

R

K

G

N

A

C

G

G

P

P

I

R

G

G

W

Y

P

P

G

G

S

M

A

A

E
|
E

V

A

V

G

N

N

M

M

Q

P

P

L

P

P

D

P

H

K

L

I

L

L

K

R

K

K

G

G

I

I

M

T

S

D

L

M

P

V

T

R

L

V

G

S

D

D

G

A

R

R

Q

M

S
|
S

G
|
G

T

T

A

A

D

Y

S

G

P

T

S

V

I

V

L

L

N

H

A

V

S

A

P

P

E

E

S

A

A

A

I

A

G

G

G

G

G

P

L

L

S

A

W

L

L

V

R

Q

T

D

G

G

D

D

T

I

I

I

R

E

I

L

D

D

L

V

N

A

T

A

G

R

R

K

C

L

D

H

A

L

L

H

V

V

D

S

E

D

A

E

T

E

I

L

A

-

A

A

R

R

K

R

Q

R

D

E

G

A

I

W

P

Q

A

A

V

P

P

P

A

A

T

P

M

M

T

A

P

R

-

G

W

W

Q

V

E

K

I

L

Y

Y

R

V

A

E

H

H

A

V

S

Q

Q

Q

L

A

D

N

T

L

G

G

G

A

V

D

L

L

E

D

F

F

A

L

V

R

K

G

Y

K

Q

S

D

-

L

-

A

G

A

A

K

G

L

I

P

P

R

K

H

D

N

N

H

H

binding bicarbonate ion: D119 (= D129), W161 (= W171), T196 (= T206), E442 (= E463), S469 (= S490), S469 (= S490), G470 (= G491)
binding fe2/s2 (inorganic) cluster: C50 (= C60), E82 (= E92), C118 (= C128), D119 (= D129), C191 (= C201)
binding magnesium ion: E82 (= E92), D119 (= D129), K120 (= K130), E442 (= E463)

8epzA Crystal structure of fe-s cluster-dependent dehydratase from paralcaligenes ureilyticus in complex with mn
41% identity, 98% coverage: 10:591/591 of query aligns to 4:569/569 of 8epzA

query
sites
8epzA

R

R

S

S

R

Q

D

A

W

W

F

F

D

G

N

R

P

L

D

D

H

R

I

D

D

G

M

F

T

-

A

-

L

I

Y

Y

L