SitesBLAST
Comparing CCNA_00994 FitnessBrowser__Caulo:CCNA_00994 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
8b7sA Crystal structure of the chloramphenicol-inactivating oxidoreductase from novosphingobium sp (see paper)
38% identity, 99% coverage: 3:552/555 of query aligns to 1:457/458 of 8b7sA
- binding flavin-adenine dinucleotide: G11 (= G13), G13 (= G15), S14 (= S16), A15 (= A17), E35 (= E37), A36 (= A38), W47 (= W74), P65 (= P92), G67 (= G94), V180 (≠ A231), A214 (≠ G265), G215 (= G266), A218 (≠ N269), T270 (≠ L348), Y391 (= Y486), A424 (= A519), I435 (≠ T530), N436 (= N531)
5nccA Structure of fatty acid photodecarboxylase in complex with fad and palmitic acid (see paper)
36% identity, 98% coverage: 8:549/555 of query aligns to 25:573/578 of 5nccA
- active site: R347 (≠ E334), L420 (≠ P400), I421 (≠ C401), S507 (≠ L485), A509 (≠ H487), G552 (= G528), Q553 (≠ N529)
- binding flavin-adenine dinucleotide: G30 (= G13), G32 (= G15), T33 (≠ S16), A34 (= A17), L53 (= L36), E54 (= E37), A55 (= A38), F74 (≠ L67), W80 (= W74), A98 (≠ P92), G100 (= G94), G105 (= G99), S106 (= S100), N110 (= N104), A111 (= A105), T112 (≠ M106), L113 (= L107), V238 (≠ A231), A278 (≠ G265), H282 (≠ N269), L286 (= L273), N508 (≠ Y486), Q553 (≠ N529), T554 (= T530), G555 (≠ N531), V558 (≠ S534)
A0A248QE08 Fatty acid photodecarboxylase, chloroplastic; CvFAP; EC 4.1.1.106 from Chlorella variabilis (Green alga) (see paper)
36% identity, 98% coverage: 8:549/555 of query aligns to 85:640/654 of A0A248QE08
- TA 93:94 (≠ SA 16:17) binding
- E114 (= E37) binding
- L162 (≠ V96) binding
- S166 (= S100) binding
- NATL 170:173 (≠ NAML 104:107) binding
- V298 (≠ A231) binding
- C432 (≠ A351) binding
- R451 (≠ H371) binding
- Y466 (vs. gap) binding
- Q486 (≠ A399) binding
- G622 (≠ N531) binding
6yrvAAA structure of fap after illumination at 100k (see paper)
36% identity, 98% coverage: 8:549/555 of query aligns to 9:564/573 of 6yrvAAA
- binding carbon dioxide: R375 (≠ H371), N499 (≠ Y486)
- binding flavin-adenine dinucleotide: G14 (= G13), G16 (= G15), T17 (≠ S16), A18 (= A17), L37 (= L36), E38 (= E37), A39 (= A38), F58 (≠ L67), W64 (= W74), A82 (≠ P92), G89 (= G99), S90 (= S100), N94 (= N104), A95 (= A105), T96 (≠ M106), L97 (= L107), M191 (≠ T200), V222 (≠ A231), C264 (≠ A264), A265 (≠ G265), G266 (= G266), H269 (≠ N269), N499 (≠ Y486), A534 (= A519), Q544 (≠ N529), T545 (= T530), G546 (≠ N531)
- binding heptadecane: V377 (≠ L373), G379 (≠ A375), M380 (= M377), G386 (vs. gap), T389 (vs. gap), Y390 (vs. gap), F393 (≠ L382), T408 (= T397), Q410 (≠ A399)
4mjwA Crystal structure of choline oxidase in complex with the reaction product glycine betaine (see paper)
35% identity, 98% coverage: 6:548/555 of query aligns to 13:529/532 of 4mjwA
- active site: I333 (≠ V353), P377 (= P400), N378 (≠ C401), V464 (≠ L485), H466 (= H487), V509 (≠ G528), N510 (= N529)
- binding flavin-adenine dinucleotide: G20 (= G13), G22 (= G15), S23 (= S16), E44 (= E37), A45 (= A38), W71 (= W74), R89 (= R93), A90 (≠ G94), G95 (= G99), C96 (≠ S100), H99 (≠ I103), N100 (= N104), S101 (≠ A105), I103 (≠ L107), R231 (≠ L230), A232 (= A231), T269 (≠ G265), G270 (= G266), D273 (≠ N269), Y465 (= Y486), H466 (= H487), A500 (= A519), N510 (= N529), P511 (≠ T530), N512 (= N531), V515 (≠ S534)
2jbvA Crystal structure of choline oxidase reveals insights into the catalytic mechanism (see paper)
35% identity, 97% coverage: 6:545/555 of query aligns to 13:526/527 of 2jbvA
- active site: I333 (≠ V353), P377 (= P400), N378 (≠ C401), V464 (≠ L485), H466 (= H487), V509 (≠ G528), N510 (= N529)
- binding [(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl (2R,3S,4S)-5-[(4aS,10aR)-7,8-dimethyl-2,4-dioxo-1,3,4,4a,5,10a-hexahydrobenzo[g]pteridin-10(2H)-yl]-2,3,4-trihydroxypentyl dihydrogen diphosphate: G22 (= G15), S23 (= S16), E44 (= E37), A45 (= A38), W71 (= W74), A90 (≠ G94), G95 (= G99), C96 (≠ S100), H99 (≠ I103), N100 (= N104), S101 (≠ A105), I103 (≠ L107), R231 (≠ L230), A232 (= A231), T269 (≠ G265), G270 (= G266), D273 (≠ N269), V464 (≠ L485), Y465 (= Y486), H466 (= H487), D499 (= D518), A500 (= A519), N510 (= N529), P511 (≠ T530), N512 (= N531), V515 (≠ S534)
3ljpA Crystal structure of choline oxidase v464a mutant (see paper)
35% identity, 98% coverage: 6:548/555 of query aligns to 13:529/530 of 3ljpA
- active site: I333 (≠ V353), P377 (= P400), N378 (≠ C401), A464 (≠ L485), H466 (= H487), V509 (≠ G528), N510 (= N529)
- binding dihydroflavine-adenine dinucleotide: G22 (= G15), S23 (= S16), E44 (= E37), A45 (= A38), W71 (= W74), R89 (= R93), A90 (≠ G94), G95 (= G99), C96 (≠ S100), H99 (≠ I103), N100 (= N104), S101 (≠ A105), I103 (≠ L107), A232 (= A231), T269 (≠ G265), D273 (≠ N269), Y465 (= Y486), H466 (= H487), D499 (= D518), A500 (= A519), N510 (= N529), P511 (≠ T530), N512 (= N531), V515 (≠ S534)
8bxlB Patulin synthase from penicillium expansum
33% identity, 96% coverage: 8:540/555 of query aligns to 15:581/590 of 8bxlB
- binding flavin-adenine dinucleotide: G20 (= G13), G22 (= G15), T23 (≠ S16), A24 (= A17), E44 (= E37), A45 (= A38), W80 (= W74), G100 (= G94), G105 (= G99), S106 (= S100), R109 (≠ I103), N110 (= N104), Y111 (≠ A105), A113 (≠ L107), L253 (= L230), A254 (= A231), A288 (≠ G265), Q292 (≠ N269), F525 (≠ Y486), D559 (= D518), A560 (= A519), H570 (≠ N529), P571 (≠ T530), Q572 (≠ N531), L575 (≠ S534)
5oc1A Crystal structure of aryl-alcohol oxidase from pleurotus eryngii in complex with p-anisic acid (see paper)
30% identity, 98% coverage: 6:547/555 of query aligns to 1:563/565 of 5oc1A
- active site: V339 (≠ A333), N413 (≠ P400), A414 (≠ C401), I499 (≠ L485), H501 (= H487), A544 (≠ G528), H545 (≠ N529)
- binding 4-methoxybenzoic acid: Y91 (≠ A105), I356 (≠ S349), I390 (≠ M377), F396 (= F383), T412 (≠ A399), I499 (≠ L485), H501 (= H487), H545 (≠ N529)
- binding flavin-adenine dinucleotide: G8 (= G13), G10 (= G15), N11 (≠ S16), A12 (= A17), E32 (= E37), A33 (= A38), W60 (= W74), P78 (= P92), G80 (= G94), G85 (= G99), S86 (= S100), H90 (≠ N104), Y91 (≠ A105), V93 (≠ L107), V230 (≠ A231), S270 (≠ A264), A271 (≠ G265), G272 (= G266), F500 (≠ Y486), H545 (≠ N529), T546 (= T530), Q547 (≠ N531), I550 (≠ S534)
3fimB Crystal structure of aryl-alcohol-oxidase from pleurotus eryingii (see paper)
30% identity, 98% coverage: 6:547/555 of query aligns to 1:563/565 of 3fimB
- active site: V339 (≠ A333), N413 (≠ P400), A414 (≠ C401), I499 (≠ L485), H501 (= H487), A544 (≠ G528), H545 (≠ N529)
- binding flavin-adenine dinucleotide: G8 (= G13), N11 (≠ S16), A12 (= A17), E32 (= E37), A33 (= A38), W60 (= W74), P78 (= P92), G80 (= G94), G85 (= G99), S86 (= S100), H90 (≠ N104), Y91 (≠ A105), V93 (≠ L107), V230 (≠ A231), S270 (≠ A264), A271 (≠ G265), F500 (≠ Y486), H501 (= H487), H545 (≠ N529), T546 (= T530), Q547 (≠ N531), I550 (≠ S534)
E4QP00 5-(hydroxymethyl)furfural oxidase; 5-hydroxymethylfurfural oxidase; HMFO; Thiol oxidase; EC 1.1.3.47; EC 1.8.3.- from Methylovorus sp. (strain MP688) (see paper)
32% identity, 98% coverage: 8:549/555 of query aligns to 7:531/531 of E4QP00
- V101 (≠ I103) mutation to H: Abolishes activity.
- M103 (≠ A105) mutation to A: 16-fold reduction in catalytic efficiency on vanillyl alcohol.
- V367 (≠ L382) mutation to K: 1.6-fold reduction in catalytic efficiency on vanillyl alcohol. Shows significantly improved activity on the aldehyde 5-formyl-2-furancarboxylate, which results in a better 5-hydroxymethylfurfural to 2,5-furandicarboxylate conversion.; mutation to R: 1.4-fold reduction in catalytic efficiency on vanillyl alcohol. Shows significantly improved activity on the aldehyde 5-formyl-2-furancarboxylate, which results in a better 5-hydroxymethylfurfural to 2,5-furandicarboxylate conversion. Displays a catalytic efficiency toward 5-formyl-2-furancarboxylate that is over 1000-fold higher than that for wild-type; when associated with F-466.
- W369 (≠ N384) mutation to A: 7.5-fold reduction in catalytic efficiency on vanillyl alcohol.
- V465 (≠ L485) mutation to A: 18-fold reduction in catalytic efficiency on vanillyl alcohol.
- W466 (≠ Y486) mutation to A: 39-fold reduction in catalytic efficiency on vanillyl alcohol. In contrast to wild-type, is active on secondary alcohols, such as (S)-1-phenylethanol, and is strictly enantionselective as this mutant has no activity on (R)-1-phenylethanol. Shows increased activity on the aldehyde 5-formyl-2-furancarboxylate.; mutation to F: 3.4-fold reduction in catalytic efficiency on vanillyl alcohol. In contrast to wild-type, is active on secondary alcohols, such as (S)-1-phenylethanol, and is strictly enantionselective as this mutant has no activity on (R)-1-phenylethanol. Shows increased activity on the aldehyde 5-formyl-2-furancarboxylate. Displays a catalytic efficiency toward 5-formyl-2-furancarboxylate that is over 1000-fold higher than that for wild-type; when associated with R-367.
- H467 (= H487) mutation to A: Abolishes activity.
- N511 (= N529) mutation to A: 53-fold reduction in catalytic efficiency on vanillyl alcohol.
4ha6A Crystal structure of pyridoxine 4-oxidase - pyridoxamine complex (see paper)
32% identity, 96% coverage: 8:542/555 of query aligns to 3:501/508 of 4ha6A
- active site: F360 (= F383), G361 (= G395), H444 (≠ L485), H446 (= H487), G487 (= G528), P488 (≠ N529)
- binding flavin-adenine dinucleotide: G8 (= G13), G10 (= G15), S11 (= S16), A12 (= A17), E32 (= E37), A33 (= A38), W58 (= W74), R77 (= R93), G78 (= G94), G83 (= G99), S84 (= S100), L87 (≠ I103), H88 (≠ N104), A89 (= A105), M90 (= M106), G91 (≠ L107), V218 (≠ A231), A251 (≠ G265), G252 (= G266), E255 (≠ N269), H445 (≠ Y486), A478 (= A519), P488 (≠ N529), I489 (≠ T530), H490 (≠ N531)
- binding 4-(aminomethyl)-5-(hydroxymethyl)-2-methylpyridin-3-ol: A89 (= A105), S314 (≠ A333), H444 (≠ L485), H446 (= H487)
4udqA Crystal structure of 5-hydroxymethylfurfural oxidase (hmfo) in the reduced state
32% identity, 97% coverage: 8:546/555 of query aligns to 3:524/525 of 4udqA
- active site: L331 (≠ V353), F364 (= F383), W365 (≠ N384), V461 (≠ L485), H463 (= H487), A506 (≠ G528), N507 (= N529)
- binding flavin-adenine dinucleotide: G8 (= G13), G10 (= G15), T11 (≠ S16), A12 (= A17), E32 (= E37), A33 (= A38), W64 (≠ D69), G88 (= G94), G93 (= G99), G94 (≠ S100), N98 (= N104), M99 (≠ A105), V101 (≠ L107), V229 (≠ A231), T261 (≠ A264), A262 (≠ G265), W462 (≠ Y486), H463 (= H487), A497 (= A519), N507 (= N529), T508 (= T530), N509 (= N531), T512 (≠ S534)
3t37A Crystal structure of pyridoxine 4-oxidase from mesorbium loti
32% identity, 96% coverage: 8:542/555 of query aligns to 3:501/509 of 3t37A
- active site: F360 (= F383), G361 (= G395), H444 (≠ L485), H446 (= H487), G487 (= G528), P488 (≠ N529)
- binding flavin-adenine dinucleotide: G8 (= G13), G10 (= G15), S11 (= S16), A12 (= A17), E32 (= E37), A33 (= A38), W58 (= W74), R77 (= R93), G78 (= G94), R79 (≠ K95), G83 (= G99), S84 (= S100), H88 (≠ N104), A89 (= A105), G91 (≠ L107), R217 (≠ L230), V218 (≠ A231), A251 (≠ G265), E255 (≠ N269), H445 (≠ Y486), A478 (= A519), P488 (≠ N529), I489 (≠ T530), H490 (≠ N531)
4h7uA Crystal structure of pyranose dehydrogenase from agaricus meleagris, wildtype (see paper)
30% identity, 97% coverage: 5:545/555 of query aligns to 14:572/577 of 4h7uA
- active site: A343 (vs. gap), V426 (≠ C401), Y510 (≠ L485), H512 (= H487), A555 (≠ G528), H556 (≠ N529)
- binding [(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl (2R,3S,4S)-2,3,4-trihydroxy-5-[(4aR)-4a-hydroxy-7,8-dimethyl-2,4-dioxo-3,4,4a,5-tetrahydrobenzo[g]pteridin-10(2H)-yl]pentyl dihydrogen diphosphate (non-preferred name): G22 (= G13), G24 (= G15), T25 (≠ S16), A26 (= A17), E46 (= E37), A47 (= A38), W74 (= W74), G99 (= G99), C100 (≠ S100), H103 (≠ I103), N104 (= N104), G105 (≠ A105), V107 (≠ L107), L242 (= L230), V243 (≠ A231), G282 (= G265), G283 (= G266), A286 (≠ N269), H512 (= H487), A546 (= A519), H556 (≠ N529), T557 (= T530), Q558 (≠ N531), V561 (≠ S534)
Q3L245 Pyranose dehydrogenase 1; PDH 1; Pyranose:quinone oxidoreductase 1; EC 1.1.99.29 from Leucoagaricus meleagris (Western flat-topped agaric) (Agaricus meleagris) (see 2 papers)
30% identity, 97% coverage: 5:545/555 of query aligns to 39:597/602 of Q3L245
- N100 (≠ L75) modified: carbohydrate, N-linked (GlcNAc...) asparagine
- H128 (≠ I103) modified: Tele-8alpha-FAD histidine
- N344 (= N302) modified: carbohydrate, N-linked (GlcNAc...) asparagine
- H537 (= H487) active site, Proton acceptor
- H581 (≠ N529) active site
Sites not aligning to the query:
4ynuA Crystal structure of aspergillus flavus fadgdh in complex with d- glucono-1,5-lactone (see paper)
31% identity, 98% coverage: 8:551/555 of query aligns to 5:568/569 of 4ynuA
- active site: V341 (≠ R330), F412 (≠ P400), W413 (≠ C401), N501 (≠ L485), H503 (= H487), G545 (= G528), H546 (≠ N529)
- binding flavin-adenine dinucleotide: G12 (= G15), T13 (≠ S16), S14 (≠ A17), E34 (= E37), A35 (= A38), Y51 (= Y63), F55 (≠ L67), W61 (= W74), R79 (≠ P92), G81 (= G94), G86 (= G99), T87 (≠ S100), N91 (= N104), G92 (≠ A105), T232 (≠ L230), A233 (= A231), A273 (≠ G265), G274 (= G266), R277 (≠ N269), F502 (≠ Y486), A536 (= A519), H546 (≠ N529), L547 (≠ T530), V548 (≠ N531), L551 (≠ S534)
- binding D-glucono-1,5-lactone: Y51 (= Y63), E411 (≠ A399), A496 (≠ Q480), N497 (≠ T481), R499 (≠ S483), R499 (≠ S483), N501 (≠ L485), H503 (= H487), H546 (≠ N529)
4yntA Crystal structure of aspergillus flavus fad glucose dehydrogenase (see paper)
31% identity, 98% coverage: 8:551/555 of query aligns to 6:569/570 of 4yntA
- active site: V342 (≠ R330), F413 (≠ P400), W414 (≠ C401), N502 (≠ L485), H504 (= H487), G546 (= G528), H547 (≠ N529)
- binding dihydroflavine-adenine dinucleotide: G13 (= G15), T14 (≠ S16), S15 (≠ A17), E35 (= E37), A36 (= A38), F56 (≠ L67), W62 (= W74), R80 (≠ P92), G82 (= G94), G87 (= G99), T88 (≠ S100), N92 (= N104), G93 (≠ A105), M94 (= M106), A95 (≠ L107), A234 (= A231), A274 (≠ G265), R278 (≠ N269), F503 (≠ Y486), A537 (= A519), H547 (≠ N529), L548 (≠ T530), V549 (≠ N531), L552 (≠ S534)
7vzsA Fad-dpendent glucose dehydrogenase complexed with an inhibitor at ph7.56
31% identity, 97% coverage: 8:545/555 of query aligns to 5:562/566 of 7vzsA
- binding D-glucal: Y6 (= Y9), L22 (= L25), N25 (= N28), Y51 (= Y63), I349 (≠ Y338), Q356 (≠ L345), E411 (≠ A399), E444 (≠ P432), W445 (≠ L433), K448 (≠ E436), R499 (≠ S483), N501 (≠ L485), H546 (≠ N529)
- binding flavin-adenine dinucleotide: G10 (= G13), G12 (= G15), T13 (≠ S16), S14 (≠ A17), E34 (= E37), A35 (= A38), Y51 (= Y63), F55 (≠ L67), W61 (= W74), R79 (≠ P92), G81 (= G94), G86 (= G99), T87 (≠ S100), N91 (= N104), G92 (≠ A105), M93 (= M106), A94 (≠ L107), T232 (≠ L230), A233 (= A231), A273 (≠ G265), G274 (= G266), R277 (≠ N269), F502 (≠ Y486), A536 (= A519), H546 (≠ N529), L547 (≠ T530), V548 (≠ N531), L551 (≠ S534)
Sites not aligning to the query:
6xuuA Crystallographic structure of oligosaccharide dehydrogenase from pycnoporus cinnabarinus, glucose-bound form (see paper)
31% identity, 97% coverage: 8:548/555 of query aligns to 16:588/589 of 6xuuA
- binding beta-D-glucopyranose: E35 (= E27), N67 (≠ K68), H83 (≠ P92), N104 (≠ A113), D108 (= D117), S139 (vs. gap), A140 (vs. gap), N142 (vs. gap), T166 (≠ A159), D169 (= D160), T188 (≠ A178), W213 (vs. gap), A225 (= A212), Y226 (= Y213), Q329 (≠ I308), N330 (≠ V309), A331 (= A310), Q357 (≠ L339), F414 (vs. gap), K474 (= K447), N517 (≠ S483), G520 (vs. gap), A522 (vs. gap), V524 (≠ L485), H526 (= H487), H569 (≠ N529)
- binding flavin-adenine dinucleotide: G21 (= G13), G23 (= G15), L24 (≠ S16), T25 (≠ A17), E45 (= E37), A46 (= A38), F66 (≠ L67), W72 (= W74), H83 (≠ P92), G85 (= G94), G90 (= G99), S91 (= S100), N95 (= N104), G96 (≠ A105), A97 (≠ M106), A98 (≠ L107), M244 (≠ L230), A245 (= A231), A286 (≠ G265), Q290 (≠ N269), A525 (≠ Y486), A559 (= A519), H569 (≠ N529), L570 (≠ T530), S571 (≠ N531), L574 (≠ S534)
- binding alpha-D-glucopyranose: G96 (≠ A105), F414 (vs. gap), W428 (≠ A399)
Query Sequence
>CCNA_00994 FitnessBrowser__Caulo:CCNA_00994
MTGIIEADYVIVGAGSAGCVLAARLSENGRYKVVLLEAGGDDRPTKNLSQFASNMMIHIP
VGYSSTLKDPKVNWLFTTEPDPGTGGRSHVWPRGKVLGGSSSINAMLYVRGQAADYDGWR
QLGCEGWAWDDVLPYFRKAQNQERGACDLHATGGPLNVADMRDAHPISEALIEACDQAGI
PRYPDLNGADQEGATWYQVTQKNGARCSSAVAYLHPAMKRPNLRVETNALAGRVLFEGKR
AVGVEFMQNGERRAAMARGEVILAGGAINSPQLLQLSGVGAGGLLREHGIEVVADLPGVG
ENLQDHYIVAARYRLKSGTVSVNEQSKGGRLAAEAMKYLLFRKGLLTLSAAHVAAFCKSR
PDLAGPDIQFHILPATMDLDKLFNEQKMELEGAPGMTIAPCQLRPESRGYIRIKSADPSV
YPAIFANYLADPLDQEVIVAGLKWARKIGQQPAIAQYVESEMNPGLEVQTDEQLLDFARQ
TGSTLYHPVGSCQMGTGPMAVVDAQLRVRGVEGLRVVDASIMPRLISGNTNAPSIMIGEK
GADMILAAARQPVAA
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory