SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing CCNA_01018 FitnessBrowser__Caulo:CCNA_01018 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q53TZ2 L-arabinose 1-dehydrogenase (NAD(P)(+)); D-galactose 1-dehydrogenase; EC 1.1.1.376; EC 1.1.1.120; EC 1.1.1.48 from Azospirillum brasilense (see paper)
54% identity, 99% coverage: 1:305/307 of query aligns to 4:307/309 of Q53TZ2

query
sites
Q53TZ2

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D169 (= D167) mutation to A: Loss of activity.
N173 (= N171) mutation to A: Decrease by 4 orders of magnitude in catalytic efficiency.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

7cgqA Crystal structure of azospirillum brasilense l-arabinose 1- dehydrogenase e147a mutant (NADP and l-arabinose bound form)
54% identity, 99% coverage: 1:305/307 of query aligns to 1:304/306 of 7cgqA

query
sites
7cgqA

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binding alpha-L-arabinopyranose: K88 (= K89), H116 (= H117), H150 (= H151), P167 (= P168), N170 (= N171)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G10 (= G10), K11 (= K11), I12 (= I12), S34 (= S34), R35 (≠ H35), H36 (≠ G36), C64 (= C65), A65 (≠ T66), P66 (= P67), V69 (= V70), E87 (= E88), K88 (= K89), H116 (= H117), Q153 (= Q154), W155 (= W156), D166 (= D167), Y263 (= Y264)

3m2tA The crystal structure of dehydrogenase from chromobacterium violaceum
34% identity, 42% coverage: 1:128/307 of query aligns to 3:136/342 of 3m2tA

query
sites
3m2tA

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binding nicotinamide-adenine-dinucleotide: G10 (= G8), G12 (= G10), A13 (≠ K11), Q14 (≠ I12), D36 (vs. gap), S37 (vs. gap), R41 (≠ H35), A73 (≠ C65), G74 (≠ T66), L78 (≠ V70), E96 (= E88), K97 (= K89), N125 (≠ H117)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 290

7x2yA Crystal structure of cis-4,5-dihydrodiol phthalate dehydrogenase in complex with NAD+ and 3-hydroxybenzoate (see paper)
35% identity, 39% coverage: 2:120/307 of query aligns to 4:126/342 of 7x2yA

query
sites
7x2yA

I

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binding 3-hydroxybenzoic acid: H122 (≠ W116)
binding nicotinamide-adenine-dinucleotide: G10 (= G8), G12 (= G10), R13 (≠ K11), A14 (= A13), D35 (≠ S34), P36 (≠ H35), R37 (≠ G36), A71 (≠ C65), S72 (≠ T66), P73 (= P67), F76 (≠ V70), Q80 (≠ T74), E94 (= E88), K95 (= K89), P96 (= P90)

Sites not aligning to the query:

Q2I8V6 1,5-anhydro-D-fructose reductase; Anhydrofructose reductase; 1,5-anhydro-D-fructose reductase (1,5-anhydro-D-mannitol-forming); EC 1.1.1.292 from Ensifer adhaerens (Sinorhizobium morelense) (see paper)
31% identity, 45% coverage: 1:138/307 of query aligns to 1:143/333 of Q2I8V6

query
sites
Q2I8V6

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ASTI 9:12 (≠ LGKI 9:12) binding
S10 (≠ G10) mutation to G: Almost no effect.
A13 (= A13) mutation to G: Can use NAD as cosubstrate as well as NADP.
S33 (≠ V30) mutation to D: No activity.
ST 33:34 (≠ VA 30:31) binding
R38 (≠ H35) binding
TTNELH 71:76 (≠ TPPQVR 66:71) binding
EK 93:94 (= EK 88:89) binding
K94 (= K89) mutation to G: Less than 1% remaining activity.
N120 (≠ S115) binding

Sites not aligning to the query:

162:163 binding
176 D→A: Less than 1% remaining activity.
180 H→A: Less than 2% remaining activity.
206 G→I: No effect.
283 binding

2glxA Crystal structure analysis of bacterial 1,5-af reductase (see paper)
37% identity, 30% coverage: 48:138/307 of query aligns to 53:142/332 of 2glxA

query
sites
2glxA

D

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active site: K93 (= K89)
binding acetate ion: K93 (= K89)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: S69 (≠ C65), T70 (= T66), N72 (≠ P68), H75 (≠ R71), E92 (= E88), K93 (= K89), H121 (= H117)

Sites not aligning to the query:

active site: 179
binding acetate ion: 179
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 7, 8, 9, 10, 11, 32, 33, 37, 161, 162, 282

7xr9A Crystal structure of dgpa with glucose (see paper)
28% identity, 45% coverage: 13:149/307 of query aligns to 16:151/344 of 7xr9A

query
sites
7xr9A

A

A

H

N

D

Q

Q

K

H

H

L

F

P

P

A

A

L

L

A

K

G

N

D

N

P

A

R

D

F

L

S

N

L

E

V

I

A

V

A

A

A

E

S

K

H

A

G

A

R

A

P

E

T

F

H

G

G

E

D

G

L

A

P

Q

V

V

F

T

H

A

D
|
D

I
x
Y

D

K

D

E

L

L

A

A

N

N

G

-

P

P

A

E

L

V

D

E

A

V

V

V

A

H

L

V

C
|
C

T
|
T

P
|
P

P

N

Q

V

V

S

R
x
H

R

S

E

E

T

I

A

T

L

I

A

A

L

F

R

E

A

A

G

G

K

K

H

H

V

V

F

Y

L

C

E
|
E

K
|
K

P
|
P

P

M

G

S

V

H

T

S

V

T

G

E

E

E

V

A

H

E

E

K

L

M

A

V

A

E

V

A

A

W

R

K

D

K

Q

S

G

G

V

K

T

Q

L

F

F

T

T

I

S

G

W

Y

H
x
Q

S

N

R

R

F

F

A

R

A

E

G

E

V

V

P

M

Q

N

A

L

K

K

A

K

W

S

L

C

A

D

K

K

R

G

R

E

L

L

R

G

K

E

L

I

A

Y

I

Y

E

G

W

K

R

A

E

H

D

A

V

V

R
|
R

R

R

binding beta-D-glucopyranose: K91 (= K89), R150 (= R148)
binding nicotinamide-adenine-dinucleotide: D51 (= D48), Y52 (≠ I49), C67 (= C65), T68 (= T66), P69 (= P67), H73 (≠ R71), E90 (= E88), K91 (= K89), P92 (= P90), Q119 (≠ H117)

Sites not aligning to the query:

binding beta-D-glucopyranose: 157, 160, 173, 177
binding nicotinamide-adenine-dinucleotide: 13, 14, 15, 159, 160, 166

4koaA Crystal structure analysis of 1,5-anhydro-d-fructose reductase from sinorhizobium meliloti (see paper)
32% identity, 45% coverage: 1:138/307 of query aligns to 1:143/333 of 4koaA

query
sites
4koaA

M

M

I

I

R

R

I

W

G

G

L

L

V

I

G
|
G

L
x
A

G
x
S

K
x
T

I
|
I

A

A

H

R

D

E

Q

W

H

V

L

I

P

G

A

A

L

I

-

R

-

A

A

A

G

G

D

G

P

E

R

V

F

V

S

S

L

V

V

M

A
x
S

A

S

S

A

H

E

G
x
R

R

G

P

E

T

A

H

Y

-

A

-

E

-

N

G

G

D

I

L

A

P

K

V

A

F

V

H

T

D

S

I

V

D

D

L

L

L

V

A

G

N

D

G

-

P

P

A

D

L

V

D

D

A

A

V

V

A

Y

L

I

C

S

T
|
T

P

T

P
x
N

Q

E

V

L

R
x
H

R

H

E

G

T

Q

A

A

L

L

A

A

L

I

R

R

A

A

G

G

K

K

H

H

V

V

F

L

L

C

E

E

K
|
K

P

P

P

L

G

A

V

M

T

N

V

L

G

N

E

D

V

G

H

C

E

E

L

M

A

V

A

L

V

K

A

A

R

C

D

E

Q

A

G

G

V

V

T

V

L

L

F

G

T

T

S

N

W

H

H

H

S

L

R

R

F

N

A

A

G

T

V

H

P

R

Q

A

A

M

K

R

A

E

W

A

L

I

A

A

K

A

R

G

R

R

L

I

R

G

K

R

active site: K94 (= K89)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G8 (= G8), A9 (≠ L9), S10 (≠ G10), T11 (≠ K11), I12 (= I12), S33 (≠ A31), S34 (≠ A32), R38 (≠ G36), T71 (= T66), N73 (≠ P68), H76 (≠ R71), K94 (= K89)

Sites not aligning to the query:

active site: 180
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 160

1rydA Crystal structure of glucose-fructose oxidoreductase from zymomonas mobilis
26% identity, 58% coverage: 6:183/307 of query aligns to 36:229/381 of 1rydA

query
sites
1rydA

L

I

V

V

G

G

L
|
L

G
|
G

K
|
K

I
x
Y

A

A

H

L

D

N

Q

Q

H

I

L

L

P

P

A

G

L

F

A

A

G

G

D

C

P

Q

R

H

F

S

S

R

L

I

V

E

A

A

A

L

A

V

S
|
S

H
x
G

G

N

R

A

P

E

T
x
K

H

A

G

K

D

I

L

V

P

A

V

A

F

E

H

Y

D

G

I

V

D

D

-

P

-

R

-

K

-

I

-

Y

-

D

-
x
Y

-

S

-

N

-

F

D

D

L

K

L

I

A

A

N

K

G

D

P

P

A

K

L

I

D

D

A

A

V

V

A

Y

L

I

C

I

T
x
L

P
|
P

P
x
N

Q

S

V
x
L

R
x
H

R

A

E

E

T

F

A

A

L

I

A

R

A

A

L

F

R

K

A

A

G

G

K

K

H

H

V

V

F

M

L

C

E
|
E

K
|
K

P

P

P

M

G

A

V

T

T

S

V

V

G

A

E

D

V

C

H

Q

E

R

L

M

A

I

A

D

V

A

A

A

R

K

D

A

Q

A

G

N

V

K

T

K

L

L

F

M

T

I

S

G

W

Y

H
x
R

S

C

R

H

F

Y

A

D

A

P

G

M

V

N

P

R

Q

A

A

A

K

V

A

K

W

L

L

I

A

R

K

E

R

N

R

Q

L

L

R

G

K

K

L

L

A

G

I

M

E

V

W

T

R

T

E

D

D

N

-

S

-

D

-

V

V

M

R

D

R

Q

W

N

H

D

P

P

G
x
A

Q

Q

A

Q

W
|
W

I
x
R

W

L

Q

R

-

R

-

E

-

L

A

A

G

G

M

G

G

S

V

L

F

M

D

D

P

I

A

G

I

I

N
x
Y

A

G

L

L

S

N

I

G

L

T

T

R

E

Y

I

L

M

L

P

G

E

E

active site: K129 (= K89), Y217 (≠ N171)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: L39 (= L9), G40 (= G10), K41 (= K11), Y42 (≠ I12), S64 (= S34), G65 (≠ H35), K69 (≠ T39), Y87 (vs. gap), L106 (≠ T66), P107 (= P67), N108 (≠ P68), L110 (≠ V70), H111 (≠ R71), E128 (= E88), K129 (= K89), R157 (≠ H117), A196 (≠ G153), W199 (= W156), R200 (≠ I157), Y217 (≠ N171)

Sites not aligning to the query:

1evjA Crystal structure of glucose-fructose oxidoreductase (gfor) delta1-22 s64d (see paper)
27% identity, 58% coverage: 6:183/307 of query aligns to 7:200/340 of 1evjA

query
sites
1evjA

L

I

V

V

G
|
G

L
|
L

G
|
G

K
|
K

I
x
Y

A

A

H

L

D

N

Q

Q

H

I

L

L

P

P

A

G

L

F

A

A

G

G

-

C

-

Q

-

H

-

S

-

R

-

I

-

E

-

A

-

L

-

V

-
x
D

-

G

D

N

P

A

R

E

F

K

S

A

L

K

V

I

A

V

A

A

S

E

H

Y

G

G

-

V

-

D

-

P

R

R

P

K

T

I

H

Y

G

-

D

D

L

Y

P

S

V

N

F

F

H

-

D

-

I

-

D

-

D

D

L

K

L

I

A

A

N

K

G

D

P

P

A

K

L

I

D

D

A

A

V

V

A

Y

L

I

C

I

T
x
L

P
|
P

P
x
N

Q

S

V

L

R
x
H

R

A

E

E

T

F

A

A

L

I

A

R

A

A

L

F

R

K

A

A

G

G

K

K

H

H

V

V

F

M

L

C

E
|
E

K
|
K

P

P

P

M

G

A

V

T

T

S

V

V

G

A

E

D

V

C

H

Q

E

R

L

M

A

I

A

D

V

A

A

A

R

K

D

A

Q

A

G

N

V

K

T

K

L

L

F

M

T

I

S

G

W

Y

H
x
R

S

C

R

H

F

Y

A

D

A

P

G

M

V

N

P

R

Q

A

A

A

K

V

A

K

W

L

L

I

A

R

K

E

R

N

R

Q

L

L

R

G

K

K

L

L

A

G

I

M

E

V

W

T

R

T

E

D

D

N

-

S

-

D

-

V

V

M

R

D

R

Q

W

N

H

D

P

P

G

A

Q

Q

A

Q

W
|
W

I
x
R

W

L

Q

R

-

R

-

E

-

L

A

A

G

G

M

G

G

S

V

L

F

M

D

D

P

I

A

G

I

I

N
x
Y

A

G

L

L

S

N

I

G

L

T

T

R

E

Y

I

L

M

L

P

G

E

E

active site: K100 (= K89), Y188 (≠ N171)
binding nicotinamide-adenine-dinucleotide: G9 (= G8), L10 (= L9), G11 (= G10), K12 (= K11), Y13 (≠ I12), D35 (vs. gap), L77 (≠ T66), P78 (= P67), N79 (≠ P68), H82 (≠ R71), E99 (= E88), K100 (= K89), R128 (≠ H117), W170 (= W156), R171 (≠ I157), Y188 (≠ N171)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 267

1h6dA Oxidized precursor form of glucose-fructose oxidoreductase from zymomonas mobilis complexed with glycerol (see paper)
26% identity, 58% coverage: 6:183/307 of query aligns to 38:231/383 of 1h6dA

query
sites
1h6dA

L

I

V

V

G
|
G

L
|
L

G
|
G

K
|
K

I
x
Y

A

A

H

L

D

N

Q

Q

H

I

L

L

P

P

A

G

L

F

A

A

G

G

D

C

P

Q

R

H

F

S

S

R

L

I

V

E

A

A

A

L

A

V

S
|
S

H
x
G

G

N

R

A

P

E

T
x
K

H

A

G

K

D

I

L

V

P

A

V

A

F

E

H

Y

D

G

I

V

D

D

-

P

-

R

-

K

-

I

-

Y

-

D

-
x
Y

-

S

-

N

-

F

D

D

L

K

L

I

A

A

N

K

G

D

P

P

A

K

L

I

D

D

A

A

V

V

A

Y

L

I

C
x
I

T
x
L

P
|
P

P
x
N

Q

S

V

L

R
x
H

R

A

E

E

T

F

A

A

L

I

A

R

A

A

L

F

R

K

A

A

G

G

K

K

H

H

V

V

F

M

L

C

E
|
E

K
|
K

P

P

P

M

G

A

V

T

T

S

V

V

G

A

E

D

V

C

H

Q

E

R

L

M

A

I

A

D

V

A

A

A

R

K

D

A

Q

A

G

N

V

K

T

K

L

L

F

M

T

I

S

G

W

Y

H
x
R

S

C

R

H

F

Y

A

D

A

P

G

M

V

N

P

R

Q

A

A

A

K

V

A

K

W

L

L

I

A

R

K

E

R

N

R

Q

L

L

R

G

K

K

L

L

A

G

I

M

E

V

W

T

R

T

E

D

D

N

-

S

-

D

-

V

V

M

R

D

R

Q

W

N

H

D

P

P

G
x
A

Q

Q

A

Q

W
|
W

I
x
R

W

L

Q

R

-

R

-

E

-

L

A

A

G

G

M

G

G

S

V

L

F

M

D
|
D

P

I

A

G

I

I

N
x
Y

A

G

L

L

S

N

I

G

L

T

T

R

E

Y

I

L

M

L

P

G

E

E

active site: K131 (= K89), Y219 (≠ N171)
binding glycerol: K131 (= K89), R202 (≠ I157), D215 (= D167), Y219 (≠ N171)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G40 (= G8), L41 (= L9), G42 (= G10), K43 (= K11), Y44 (≠ I12), S66 (= S34), G67 (≠ H35), K71 (≠ T39), Y89 (vs. gap), I107 (≠ C65), L108 (≠ T66), P109 (= P67), N110 (≠ P68), H113 (≠ R71), E130 (= E88), K131 (= K89), R159 (≠ H117), A198 (≠ G153), W201 (= W156), R202 (≠ I157), Y219 (≠ N171)

Sites not aligning to the query:

binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 17, 18, 19, 298

7xreC Crystal structure of dgpa
27% identity, 45% coverage: 13:149/307 of query aligns to 16:161/363 of 7xreC

query
sites
7xreC

A

A

H

N

D

Q

Q

K

H

H

L

F

P

P

A

A

L

L

A

K

G

N

D

N

P

A

R

D

F

L

S

N

L

E

V

I

A

V

A

A

A

F

S

C

H
x
D

G
x
I

R

Q

P

I

T

D

H

R

G

A

D

E

-

K

-

A

-

E

-

F

-

G

-

A

-

E

-

G

L

A

P

Q

V

V

F

T

H

A

D

D

I

Y

D

K

D

E

L

L

A

A

N

N

G

-

P

P

A

E

L

V

D

E

A

V

V

V

A

H

L

V

C

C

T
|
T

P
|
P

P
x
N

Q

V

V

S

R
x
H

R

S

E

E

T

I

A

T

L

I

A

A

L

F

R

E

A

A

G

G

K

K

H

H

V

V

F

Y

L

C

E
|
E

K
|
K

P
|
P

P

M

G

S

V

H

T

S

V

T

G

E

E

E

V

A

H

E

E

K

L

M

A

V

A

E

V

A

A

W

R

K

D

K

Q

S

G

G

V

K

T

Q

L

F

F

T

T

I

S

G

W

Y

H

Q

S

N

R

R

F

F

A

R

A

E

G

E

V

V

P

M

Q

N

A

L

K

K

A

K

W

S

L

C

A

D

K

K

R

G

R

E

L

L

R

G

K

E

L

I

A

Y

I

Y

E

G

W

K

R

A

E

H

D

A

V

V

R

R

binding nicotinamide-adenine-dinucleotide: D38 (≠ H35), I39 (≠ G36), T78 (= T66), P79 (= P67), N80 (≠ P68), H83 (≠ R71), E100 (= E88), K101 (= K89), P102 (= P90)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 13, 14, 170, 176, 187, 313, 314

1zh8A Crystal structure of oxidoreductase (tm0312) from thermotoga maritima at 2.50 a resolution
21% identity, 91% coverage: 2:279/307 of query aligns to 4:291/325 of 1zh8A

query
sites
1zh8A

I

I

R

R

I

L

G

G

L

I

V

V

G
|
G

L
x
C

G
|
G

K
x
I

I
x
A

A

A

H

R

D

E

Q

L

H

H

L

L

P

P

A

A

L

L

A

K

G

N

D

L

P

S

R

H

F

L

S

F

L

E

V

I

A

T

A

A

A

V

S

T

H
x
S

G
x
R

R
x
T

P

R

T

S

H
|
H

-

A

-

E

-

F

-

A

-

K

-

M

-

V

G

G

D

N

L

P

P

A

V

V

F

F

H

D

D

S

I

Y

D

E

L

L

A

E

N

S

G

G

P

-

A

L

L

V

D

D

A

A

V

V

A

D

L

L

C
x
T

T
x
L

P
|
P

P

V

Q

E

V
x
L

R

N

R

L

E

P

T

F

A

I

L

E

A

K

A

A

L

L

R

R

A

K

G

G

K

V

H

H

V

V

F

I

L

C

E
|
E

K
|
K

P

P

P

I

G

S

V

T

T

D

V

V

G

E

E

T

V

G

H

K

E

K

L

V

A

V

A

E

V

L

A

S

R

E

D

K

Q

S

G

E

V

K

T

T

L

V

F

Y

T

I

S

A

W

E

H
x
N

S

F

R

R

F

H

A

V

A

P

G

A

V

F

P

-

Q

-

A

-

K

-

A

-

W

W

L

K

A

A

K

K

R

E

R

L

L

V

R

E

K

S

L

G

A

A

I

I

-

G

-

D

-

P

-

V

-

F

-

M

E

N

W

W

R

Q

-

I

-

W

-

V

-

G

E

M

D

D

V

E

R

N

W

K

H
x
Y

P

V

G

H

Q

T

A

D

W
|
W

I
x
R

W

K

Q

K

A

P

G

K

G

H

M

V

G

G

V

G

F

F

-

L

-

S

D

D

P

G

A

G

I

V

N
x
H

A

H

L

A

S

A

I

A

L

M

T

R

E

L

I

I

M

L

P

G

E

E

E

I

V

E

F

W

V

I

T

S

G

A

A

V

K

A

L

K

Q

D

V

L

P

-

E

-

N

-

V

-

Q

-

A

S

P

P

I

L

A

L

G

G

H

G

V

M

D

D

I

F

T

L

T

S

L

S

-

I

-

F

-

E

-

F

-

E

S

N

G

G

V

T

P

V

V

G

T

N

A

Y

D

T

L

I

D

S

F

Y

L

S

Q

L

T

K

G

G

P

N

Q

E

T

R

W

F

D

E

I

I

T

T

A

G

W

T

T

K

D

G

D

-

G

-

V

-

L

-

K

K

L

I

S

S

L

I

G

S

G

W

A

D

E

K

L

I

T

V

I

L

D

N

G

E

Q

E

A

E

I

M

P

K

V

V

G

P

E

Q

N

E

R

N

E

S

Y
|
Y

P

Q

G

K

L

E

Y

F

D

E

R

D

F

F

A

Y

T

Q

L

V

I

V

A

A

E

E

G

G

R

K

active site: K98 (= K89), H187 (≠ N171)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G10 (= G8), C11 (≠ L9), G12 (= G10), I13 (≠ K11), A14 (≠ I12), S37 (≠ H35), R38 (≠ G36), T39 (≠ R37), H42 (= H40), T74 (≠ C65), L75 (≠ T66), P76 (= P67), L79 (≠ V70), E97 (= E88), K98 (= K89), N126 (≠ H117), Y165 (≠ H151), W170 (= W156), R171 (≠ I157), H187 (≠ N171), Y276 (= Y264)

5a06A Crystal structure of aldose-aldose oxidoreductase from caulobacter crescentus complexed with sorbitol (see paper)
32% identity, 38% coverage: 6:122/307 of query aligns to 8:134/336 of 5a06A

query
sites
5a06A

L

I

V

L

G

G

L
|
L

G
|
G

K
x
Y

I
x
Y

A

A

H

T

D

R

Q

I

H

I

L

M

P

P

A

R

L

F

A

A

G

-

D

E

P

C

R

E

F

H

S

S

L

R

V

L

A

A

A

L

S

V

H
x
S

G
|
G

R
x
T

P

P

-

E

-
x
K

-

L

-

K

T

T

H

Y

G

G

D

E

-

Q

-

Y

-

G

L

I

P

P

V

E

F

T

H

H

D

R

I

Y

D

S

-
x
Y

-

E

-

T

-

F

D

D

L

R

L

I

A

I

N

D

G

N

P

P

A

D

L

V

D
|
D

A

I

V

V

A

Y

L

V

C
x
I

T
|
T

P
|
P

P
x
N

Q

S

V
x
L

R
x
H

R

R

E

P

T

F

A

T

L

E

A

R

A

A

L

A

R

R

A

A

G

G

K

K

H
|
H

V

V

F

M

L

C

E
|
E

K
|
K

P

P

P

M

G

A

V

N

T

T

V

V

G

A

E

D

V

C

H

E

E

A

L

M

A

I

A

A

V

A

A

C

R

K

D

K

Q

A

G

G

V
x
R

T

K

L
|
L

F

M

T

I

S

G

W

Y

H
x
R

S

S

R

R

F

F

A

Q

A

A

active site: K101 (= K89)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: L11 (= L9), G12 (= G10), Y13 (≠ K11), Y14 (≠ I12), S36 (≠ H35), G37 (= G36), T38 (≠ R37), K41 (vs. gap), Y59 (vs. gap), I77 (≠ C65), T78 (= T66), P79 (= P67), N80 (≠ P68), L82 (≠ V70), H83 (≠ R71), E100 (= E88), K101 (= K89), R129 (≠ H117)
binding sorbitol: D72 (= D60), H96 (= H84), K101 (= K89), R122 (≠ V110), R122 (≠ V110), L124 (= L112)

Sites not aligning to the query:

active site: 186
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 168, 169, 186, 264
binding sorbitol: 2, 160, 169, 182, 186, 287, 296, 299, 306, 310, 311

5a03C Crystal structure of aldose-aldose oxidoreductase from caulobacter crescentus complexed with xylose (see paper)
32% identity, 38% coverage: 6:122/307 of query aligns to 8:134/336 of 5a03C

query
sites
5a03C

L

I

V

L

G
|
G

L
|
L

G
|
G

K
x
Y

I
x
Y

A

A

H

T

D

R

Q

I

H

I

L

M

P

P

A

R

L

F

A

A

G

-

D

E

P

C

R

E

F

H

S

S

L

R

V

L

A

A

A

L

S

V

H
x
S

G
|
G

R
x
T

P

P

-

E

-
x
K

-

L

-

K

T

T

H

Y

G

G

D

E

-

Q

-

Y

-

G

L

I

P

P

V

E

F

T

H

H

D

R

I

Y

D

S

-
x
Y

-

E

-

T

-

F

D

D

L

R

L

I

A

I

N

D

G

N

P

P

A

D

L

V

D

D

A

I

V

V

A

Y

L

V

C
x
I

T
|
T

P
|
P

P
x
N

Q

S

V
x
L

R
x
H

R

R

E

P

T

F

A

T

L

E

A

R

A

A

L

A

R

R

A

A

G

G

K

K

H

H

V

V

F

M

L

C

E
|
E

K
|
K

P

P

P

M

G

A

V

N

T

T

V

V

G

A

E

D

V

C

H

E

E

A

L

M

A

I

A

A

V

A

A

C

R

K

D

K

Q

A

G

G

V

R

T

K

L

L

F

M

T

I

S

G

W

Y

H
x
R

S

S

R

R

F

F

A

Q

A

A

active site: K101 (= K89)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G10 (= G8), L11 (= L9), G12 (= G10), Y13 (≠ K11), Y14 (≠ I12), S36 (≠ H35), G37 (= G36), T38 (≠ R37), K41 (vs. gap), Y59 (vs. gap), I77 (≠ C65), T78 (= T66), P79 (= P67), N80 (≠ P68), L82 (≠ V70), H83 (≠ R71), E100 (= E88), K101 (= K89), R129 (≠ H117)
binding beta-D-xylopyranose: K101 (= K89)

Sites not aligning to the query:

active site: 186
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 168, 169, 186, 264
binding beta-D-xylopyranose: 160, 169, 182, 186

5a05A Crystal structure of aldose-aldose oxidoreductase from caulobacter crescentus complexed with maltotriose (see paper)
32% identity, 38% coverage: 6:122/307 of query aligns to 7:133/335 of 5a05A

query
sites
5a05A

L

I

V

L

G
|
G

L
|
L

G
|
G

K
x
Y

I
x
Y

A

A

H

T

D

R

Q

I

H

I

L

M

P

P

A

R

L

F

A

A

G

-

D

E

P

C

R

E

F

H

S

S

L

R

V

L

A

A

A

L

S

V

H
x
S

G
|
G

R
x
T

P

P

-

E

-
x
K

-

L

-

K

T

T

H

Y

G

G

D

E

-

Q

-

Y

-

G

L

I

P

P

V

E

F

T

H

H

D

R

I

Y

D

S

-
x
Y

-

E

-

T

-

F

D

D

L

R

L

I

A

I

N

D

G

N

P

P

A

D

L

V

D

D

A

I

V

V

A

Y

L

V

C
x
I

T
|
T

P
|
P

P
x
N

Q

S

V
x
L

R
x
H

R

R

E

P

T

F

A

T

L

E

A

R

A

A

L

A

R

R

A

A

G

G

K

K

H

H

V

V

F

M

L

C

E
|
E

K
|
K

P

P

P

M

G

A

V

N

T

T

V

V

G

A

E

D

V

C

H

E

E

A

L

M

A

I

A

A

V

A

A

C

R

K

D

K

Q

A

G

G

V

R

T

K

L

L

F

M

T

I

S

G

W

Y

H
x
R

S

S

R

R

F

F

A

Q

A

A

active site: K100 (= K89)
binding beta-D-glucopyranose: K100 (= K89)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G9 (= G8), L10 (= L9), G11 (= G10), Y12 (≠ K11), Y13 (≠ I12), S35 (≠ H35), G36 (= G36), T37 (≠ R37), K40 (vs. gap), Y58 (vs. gap), I76 (≠ C65), T77 (= T66), P78 (= P67), N79 (≠ P68), L81 (≠ V70), H82 (≠ R71), E99 (= E88), K100 (= K89), R128 (≠ H117)

Sites not aligning to the query:

active site: 185
binding beta-D-glucopyranose: 159, 181, 185
binding alpha-D-glucopyranose: 259, 262
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 167, 168, 185, 263

5a04A Crystal structure of aldose-aldose oxidoreductase from caulobacter crescentus complexed with glucose (see paper)
32% identity, 38% coverage: 6:122/307 of query aligns to 7:133/335 of 5a04A

query
sites
5a04A

L

I

V

L

G

G

L
|
L

G
|
G

K
x
Y

I
x
Y

A

A

H

T

D

R

Q

I

H

I

L

M

P

P

A

R

L

F

A

A

G

-

D

E

P

C

R

E

F

H

S

S

L

R

V

L

A

A

A

L

S

V

H
x
S

G
|
G

R
x
T

P

P

-

E

-
x
K

-

L

-

K

T

T

H

Y

G

G

D

E

-

Q

-

Y

-

G

L

I

P

P

V

E

F

T

H

H

D

R

I

Y

D

S

-
x
Y

-

E

-

T

-

F

D

D

L

R

L

I

A

I

N

D

G

N

P

P

A

D

L

V

D

D

A

I

V

V

A

Y

L

V

C
x
I

T
|
T

P
|
P

P
x
N

Q

S

V
x
L

R
x
H

R

R

E

P

T

F

A

T

L

E

A

R

A

A

L

A

R

R

A

A

G

G

K

K

H

H

V

V

F

M

L

C

E
|
E

K
|
K

P

P

P

M

G

A

V

N

T

T

V

V

G

A

E

D

V

C

H

E

E

A

L

M

A

I

A

A

V

A

A

C

R

K

D

K

Q

A

G

G

V

R

T

K

L

L

F

M

T

I

S

G

W

Y

H
x
R

S

S

R

R

F

F

A

Q

A

A

active site: K100 (= K89)
binding beta-D-glucopyranose: K100 (= K89)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: L10 (= L9), G11 (= G10), Y12 (≠ K11), Y13 (≠ I12), S35 (≠ H35), G36 (= G36), T37 (≠ R37), K40 (vs. gap), Y58 (vs. gap), I76 (≠ C65), T77 (= T66), P78 (= P67), N79 (≠ P68), L81 (≠ V70), H82 (≠ R71), E99 (= E88), K100 (= K89), R128 (≠ H117)

Sites not aligning to the query:

active site: 185
binding beta-D-glucopyranose: 159, 168, 181, 185
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 167, 168, 185, 263

5a03E Crystal structure of aldose-aldose oxidoreductase from caulobacter crescentus complexed with xylose (see paper)
32% identity, 38% coverage: 6:122/307 of query aligns to 7:133/335 of 5a03E

query
sites
5a03E

L

I

V

L

G
|
G

L
|
L

G
|
G

K
x
Y

I
x
Y

A

A

H

T

D

R

Q

I

H

I

L

M

P

P

A

R

L

F

A

A

G

-

D

E

P

C

R

E

F

H

S

S

L

R

V

L

A

A

A

L

S

V

H
x
S

G
|
G

R
x
T

P

P

-

E

-
x
K

-

L

-

K

T

T

H

Y

G

G

D

E

-

Q

-

Y

-

G

L

I

P

P

V

E

F

T

H

H

D

R

I

Y

D

S

-
x
Y

-

E

-

T

-

F

D

D

L

R

L

I

A

I

N

D

G

N

P

P

A

D

L

V

D

D

A

I

V

V

A

Y

L

V

C
x
I

T
|
T

P
|
P

P
x
N

Q

S

V

L

R
x
H

R

R

E

P

T

F

A

T

L

E

A

R

A

A

L

A

R

R

A

A

G

G

K

K

H

H

V

V

F

M

L

C

E
|
E

K
|
K

P

P

P

M

G

A

V

N

T

T

V

V

G

A

E

D

V

C

H

E

E

A

L

M

A

I

A

A

V

A

A

C

R

K

D

K

Q

A

G

G

V

R

T

K

L

L

F

M

T

I

S

G

W

Y

H
x
R

S

S

R

R

F

F

A

Q

A

A

active site: K100 (= K89)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G9 (= G8), L10 (= L9), G11 (= G10), Y12 (≠ K11), Y13 (≠ I12), S35 (≠ H35), G36 (= G36), T37 (≠ R37), K40 (vs. gap), Y58 (vs. gap), I76 (≠ C65), T77 (= T66), P78 (= P67), N79 (≠ P68), H82 (≠ R71), E99 (= E88), K100 (= K89), R128 (≠ H117)
binding beta-D-xylopyranose: K100 (= K89)

Sites not aligning to the query:

active site: 185
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 167, 168, 185, 263
binding beta-D-xylopyranose: 159, 168, 181, 185, 205, 207, 209
binding alpha-D-xylopyranose: 134, 268, 279, 280

5a02A Crystal structure of aldose-aldose oxidoreductase from caulobacter crescentus complexed with glycerol (see paper)
32% identity, 38% coverage: 6:122/307 of query aligns to 7:133/335 of 5a02A

query
sites
5a02A

L

I

V

L

G

G

L
|
L

G
|
G

K
x
Y

I
x
Y

A

A

H

T

D

R

Q

I

H

I

L

M

P

P

A

R

L

F

A

A

G

-

D

E

P

C

R

E

F

H

S

S

L

R

V

L

A

A

A

L

S

V

H
x
S

G
|
G

R
x
T

P

P

-

E

-
x
K

-

L

-

K

T

T

H

Y

G

G

D

E

-

Q

-

Y

-

G

L

I

P

P

V

E

F

T

H

H

D

R

I

Y

D

S

-
x
Y

-

E

-

T

-

F

D

D

L

R

L

I

A

I

N

D

G

N

P

P

A

D

L

V

D

D

A

I

V

V

A

Y

L

V

C
x
I

T
|
T

P
|
P

P

N

Q

S

V
x
L

R
x
H

R

R

E

P

T

F

A

T

L

E

A

R

A

A

L

A

R

R

A

A

G

G

K

K

H

H

V

V

F

M

L

C

E
|
E

K
|
K

P

P

P

M

G

A

V

N

T

T

V

V

G

A

E

D

V

C

H

E

E

A

L

M

A

I

A

A

V

A

A

C

R

K

D

K

Q

A

G

G

V

R

T

K

L

L

F

M

T

I

S

G

W

Y

H
x
R

S

S

R

R

F

F

A

Q

A

A

active site: K100 (= K89)
binding nadp nicotinamide-adenine-dinucleotide phosphate: L10 (= L9), G11 (= G10), Y12 (≠ K11), Y13 (≠ I12), S35 (≠ H35), G36 (= G36), T37 (≠ R37), K40 (vs. gap), Y58 (vs. gap), I76 (≠ C65), T77 (= T66), P78 (= P67), L81 (≠ V70), H82 (≠ R71), E99 (= E88), K100 (= K89), R128 (≠ H117)

Sites not aligning to the query:

active site: 185
binding nadp nicotinamide-adenine-dinucleotide phosphate: 167, 168, 185, 263

4n54A Crystal structure of scyllo-inositol dehydrogenase from lactobacillus casei with bound cofactor NAD(h) and scyllo-inositol
28% identity, 44% coverage: 2:136/307 of query aligns to 3:144/340 of 4n54A

query
sites
4n54A

I

I

R

K

I

I

G

G

L

I

V
|
V

G
|
G

L
|
L

G
|
G

K
x
R

I
x
L

A

G

H

K

D

-

Q

I

H

H

L

A

P

T

A

N

L

I

A

A

G

T

D

K

P

I

R

Q

F

H

S

A

L

K

V

L

A

Q

A

A

S

T

H
x
S

G
x
V

R

V

P

P

T

A

H
x
E

G

L

D

D

-

W

-

A

-

K

-

E

-

L

-

G

-

V

L

E

P

E

V

V

F

F

H

E

D

D

I

F

D

D

L

M

L

V

A

Q

N

H

G

A

P

D

A

-

L

I

D

D

A

A

V

V

A

F

L

I

C

V

T
x
S

P
|
P

P

S

Q

G

V
x
F

R
x
H

R

L

E

Q

T

Q

A

I

L

E

A

S

A

A

L

L

R

N

A

A

G

G

K

K

H

H

V

V

F

F

L

S

E
|
E

K
|
K

P

P

P

I

G

G

V

L

T

D

V

I

G

E

E

A

V

I

-

E

H

H

E

T

L

Q

A

Q

A

V

V

I

A

A

R

Q

D

H

Q

A

G

N

V

L

T

K

L

F

F

Q

T

L

S

G

W

F

H
x
M

S

R

R

R

F

F

A

D

G

S

V

Y

P

R

Q

Y

A

A

K

K

A

Q

W

L

L

V

A

D

K

Q

R

G

R

K

L

I

active site: K96 (= K89)
binding (1r,2r,3r,4r,5r,6r)-cyclohexane-1,2,3,4,5,6-hexol: R12 (≠ K11), K96 (= K89)
binding 1,4-dihydronicotinamide adenine dinucleotide: V8 (= V7), G9 (= G8), L10 (= L9), G11 (= G10), R12 (≠ K11), L13 (≠ I12), S35 (≠ H35), V36 (≠ G36), E40 (≠ H40), S73 (≠ T66), P74 (= P67), F77 (≠ V70), H78 (≠ R71), E95 (= E88), K96 (= K89), M125 (≠ H117)

Sites not aligning to the query:

active site: 183
binding (1r,2r,3r,4r,5r,6r)-cyclohexane-1,2,3,4,5,6-hexol: 156, 179, 180, 183, 238, 244
binding 1,4-dihydronicotinamide adenine dinucleotide: 167, 284

Query Sequence

>CCNA_01018 FitnessBrowser__Caulo:CCNA_01018
MIRIGLVGLGKIAHDQHLPALAGDPRFSLVAAASHGRPTHGDLPVFHDIDDLLANGPALD
AVALCTPPQVRRETALAALRAGKHVFLEKPPGVTVGEVHELAAVARDQGVTLFTSWHSRF
AAGVPQAKAWLAKRRLRKLAIEWREDVRRWHPGQAWIWQAGGMGVFDPAINALSILTEIM
PEEVFVTGAKLQVPENVQAPIAGHVDITTLSGVPVTADLDFLQTGPQTWDITAWTDDGVL
KLSLGGAELTIDGQAIPVGENREYPGLYDRFATLIAEGRSEVDVRPLSLVADAFLVGDRE
RVEAFVE

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory