SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing CCNA_01268 FitnessBrowser__Caulo:CCNA_01268 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P00043 Cytochrome c from Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990 / NBRC 0083 / IGC 2968) (Yeast) (Torulaspora hansenii) (see paper)
49% identity, 62% coverage: 54:157/168 of query aligns to 1:105/110 of P00043

query
sites
P00043

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P

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A

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Y

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M1 (≠ L54) modified: Initiator methionine, Removed
K79 (≠ R131) modified: N6,N6,N6-trimethyllysine

P00025 Cytochrome c from Katsuwonus pelamis (Skipjack tuna) (Bonito) (see 2 papers)
49% identity, 60% coverage: 61:161/168 of query aligns to 2:103/104 of P00025

query
sites
P00025

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G2 (= G61) modified: N-acetylglycine
C15 (= C73) binding covalent
C18 (= C76) binding covalent
H19 (= H77) binding axial binding residue
M81 (= M139) binding axial binding residue

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

1cycA The crystal structure of bonito (katsuo) ferrocytochromE C at 2.3 angstroms resolution. Ii. Structure and function (see paper)
49% identity, 60% coverage: 61:161/168 of query aligns to 1:102/103 of 1cycA

query
sites
1cycA

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binding heme c: K13 (vs. gap), C14 (= C73), C17 (= C76), H18 (= H77), L32 (= L91), R38 (= R97), Y46 (= Y105), Y48 (≠ F107), N52 (≠ V111), T78 (= T137), K79 (= K138), M80 (= M139), F82 (= F141)

P67882 Cytochrome c from Meleagris gallopavo (Wild turkey)
51% identity, 58% coverage: 61:158/168 of query aligns to 2:100/105 of P67882

query
sites
P67882

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G2 (= G61) modified: N-acetylglycine

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

P67881 Cytochrome c from Gallus gallus (Chicken) (see paper)
51% identity, 58% coverage: 61:158/168 of query aligns to 2:100/105 of P67881

query
sites
P67881

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G2 (= G61) modified: N-acetylglycine
C15 (= C73) binding covalent
C18 (= C76) binding covalent

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

1lfmA Crystal structure of cobalt(iii)-substituted cytochromE C (tuna) (see paper)
49% identity, 60% coverage: 61:161/168 of query aligns to 1:102/103 of 1lfmA

query
sites
1lfmA

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binding protoporphyrin ix containing co: C14 (= C73), C17 (= C76), H18 (= H77), V28 (≠ T87), G29 (= G88), P30 (= P89), G41 (= G100), Y48 (≠ F107), T49 (≠ S108), N52 (≠ V111), W59 (= W118), Y67 (≠ W126), T78 (= T137), K79 (= K138), M80 (= M139), F82 (= F141)

1i54A CytochromE C (tuna) 2fe:1zn mixed-metal porphyrins (see paper)
49% identity, 60% coverage: 61:161/168 of query aligns to 1:102/103 of 1i54A

query
sites
1i54A

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binding heme c: C14 (= C73), C17 (= C76), H18 (= H77), V28 (≠ T87), G29 (= G88), P30 (= P89), G41 (= G100), Y46 (= Y105), Y48 (≠ F107), T49 (≠ S108), N52 (≠ V111), W59 (= W118), Y67 (≠ W126), L68 (= L127), T78 (= T137), K79 (= K138), M80 (= M139), F82 (= F141)
binding protoporphyrin ix containing zn: C14 (= C73), C17 (= C76), H18 (= H77), G29 (= G88), P30 (= P89), G41 (= G100), Y46 (= Y105), Y48 (≠ F107), T49 (≠ S108), N52 (≠ V111), W59 (= W118), Y67 (≠ W126), L68 (= L127), T78 (= T137), K79 (= K138), M80 (= M139), F82 (= F141)

1criA The role of a conserved internal water molecule and its associated hydrogen bond network in cytochromE C (see paper)
51% identity, 53% coverage: 73:161/168 of query aligns to 19:107/108 of 1criA

query
sites
1criA

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binding protoporphyrin ix containing fe: C19 (= C73), C22 (= C76), H23 (= H77), I40 (≠ V94), S45 (≠ A99), Y51 (= Y105), Y53 (≠ F107), T54 (≠ S108), I57 (≠ V111), W64 (= W118), M69 (≠ L123), T83 (= T137), K84 (= K138), M85 (= M139), F87 (= F141)

Sites not aligning to the query:

binding protoporphyrin ix containing fe: 18

P68519 Cytochrome c from Crotalus viridis viridis (Prairie rattlesnake) (see paper)
50% identity, 60% coverage: 61:161/168 of query aligns to 2:103/105 of P68519

query
sites
P68519

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G2 (= G61) modified: N-acetylglycine

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

P68518 Cytochrome c from Crotalus atrox (Western diamondback rattlesnake) (see paper)
50% identity, 60% coverage: 61:161/168 of query aligns to 2:103/105 of P68518

query
sites
P68518

G
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G2 (= G61) modified: N-acetylglycine

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

P68517 Cytochrome c from Crotalus adamanteus (Eastern diamondback rattlesnake) (see paper)
50% identity, 60% coverage: 61:161/168 of query aligns to 2:103/105 of P68517

query
sites
P68517

G
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K

K

A

T

G

G

V

Q

K

A

E

V

G

G

Y

Y

S

S

F

Y

S

T

D

A

A

A

V

N

K

K

A

N

A

K

G

G

F

I

I

I

W

W

D

G

A

D

Q

D

Q

T

L

L

D

M

K

E

W

Y

L

L

A

E

D

N

P

P

R

K

G

K

F

Y

M

I

P

P

G

G

T

T

K

K

M

M

T

V

F

F

A

T

G

G

I

L

K

K

N

S

E

K

K

K

D

E

R

R

I

T

D

D

L

L

I

I

A

A

Y

Y

L

L

K

K

V

E

E

A

T

T

G2 (= G61) modified: N-acetylglycine

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

P00047 Cytochrome c from Thermomyces lanuginosus (Humicola lanuginosa) (see paper)
50% identity, 62% coverage: 58:161/168 of query aligns to 6:110/111 of P00047

query
sites
P00047

Y

F

N

E

T

P

G

G

D

D

L

A

S

S

N

K

G

G

Q

A

S

N

K

L

F

F

-

K

A

T

L

R

C
|
C

R

A

S

Q

C
|
C

H

H

T

S

I

V

T

E

E

Q

G

G

P

A

D

N

L

K

T

I

G

G

P

P

N

N

L

L

Y

H

G

G

V

L

F

F

G

G

R

R

K

K

A

T

G

G

V

S

K

V

E

E

G

G

Y

Y

S

S

F

Y

S

T

D

D

A

A

V

N

K

K

A

Q

A

A

G

G

F

I

I

T

W

W

D

N

A

E

Q

D

Q

T

L

L

D

F

K

E

W

Y

L

L

A

E

D

N

P

P

R
x
K

G

K

F

F

M

I

P

P

G

G

T

T

K

K

M

M

T

A

F

F

A

G

G

G

I

L

K
|
K

N

K

E

N

K

K

D

D

R

R

I

N

D

D

L

L

I

I

A

T

Y

Y

L

L

K

K

V

E

E

A

T

T

C22 (= C73) binding covalent
C25 (= C76) binding covalent
K80 (≠ R131) modified: N6,N6-dimethyllysine
K94 (= K145) modified: N6,N6,N6-trimethyllysine; partial

P00017 Cytochrome c from Aptenodytes patagonicus (King penguin)
51% identity, 58% coverage: 61:158/168 of query aligns to 2:100/105 of P00017

query
sites
P00017

G
|
G

D

D

L

I

S

E

N

K

G

G

Q

K

S

K

K

I

F

F

A

V

L

Q

-

K

C

C

R

S

S

Q

C

C

H

H

T

T

I

V

T

E

E

K

G

G

P

K

D

H

L

K

T

T

G

G

P

P

N

N

L

L

Y

H

G

G

V

I

F

F

G

G

R

R

K

K

A

T

G

G

V

Q

K

A

E

E

G

G

Y

F

S

S

F

Y

S

T

D

D

A

A

V

N

K

K

A

N

A

K

G

G

F

I

I

T

W

W

D

G

A

E

Q

D

Q

T

L

L

D

M

K

E

W

Y

L

L

A

E

D

N

P

P

R

K

G

K

F

Y

M

I

P

P

G

G

T

T

K

K

M

M

T

I

F

F

A

A

G

G

I

I

K

K

N

K

E

K

K

S

D

E

R

R

I

A

D

D

L

L

I

I

A

A

Y

Y

L

L

K

K

G2 (= G61) modified: N-acetylglycine

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

7pr2A Cocrystal of cytochromE C and sulfonato-thiacalix[4]arene (see paper)
46% identity, 63% coverage: 56:161/168 of query aligns to 1:107/108 of 7pr2A

query
sites
7pr2A

A

A

P

E

Y

F

N

K

T

A

G

G

D

S

L

A

S

K

N

K

G

G

Q

A

S

T

K

L

F

F

-

K

A

T

L
x
R

C
|
C

R

L

S
x
Q

C
|
C

H
|
H

T

T

I

V

T

E

E

K

G

G

P

P

D

H

L

K

T

V

G
|
G

P
|
P

N

N

L

L

Y

H

G

G

V
x
I

F

F

G

G

R

R

K

H

A
x
S

G
|
G

V

Q

K

A

E

E

G

G

Y
|
Y

S

S

F
x
Y

S
x
T

D

D

A

A

V
x
N

K

I

A

K

G

N

F

V

I

L

W

W

D

D

A

E

Q

N

L
x
M

D

S

K

E

W
x
Y

L
|
L

A

T

D

N

P

P

R

K

G

K

F

Y

M

I

P

P

G

G

T
|
T

K
|
K

M
|
M

T

A

F
|
F

A

G

G

G

I

L

K
|
K

N
x
K

E

E

K

K

D

D

R

R

I

N

D

D

L

L

I

I

A

T

Y

Y

L

L

K

K

V

K

E

A

T

T

binding sulfonato-thiacalix[4]arene: R18 (≠ L72), K91 (= K145), K92 (≠ N146)
binding heme c: C19 (= C73), Q21 (≠ S75), C22 (= C76), H23 (= H77), G34 (= G88), P35 (= P89), I40 (≠ V94), S45 (≠ A99), G46 (= G100), Y51 (= Y105), Y53 (≠ F107), T54 (≠ S108), N57 (≠ V111), M69 (≠ L123), Y72 (≠ W126), L73 (= L127), T83 (= T137), K84 (= K138), M85 (= M139), F87 (= F141)

6gd6A CytochromE C in complex with sulfonato-calix[8]arene, h3 form with ammonium sulfate (see paper)
46% identity, 63% coverage: 56:161/168 of query aligns to 1:107/108 of 6gd6A

query
sites
6gd6A

A

A

P

E

Y

F

N

K

T

A

G

G

D
x
S

L
x
A

S
x
K

N

K

G

G

Q
x
A

S

T

K

L

F

F

-

K

A

T

L
x
R

C
|
C

R

L

S

Q

C
|
C

H
|
H

T

T

I

V

T

E

E

K

G

G

P

P

D

H

L

K

T

V

G
|
G

P
|
P

N

N

L

L

Y

H

G

G

V
x
I

F

F

G

G

R

R

K

H

A
x
S

G
|
G

V

Q

K

A

E

E

G

G

Y
|
Y

S

S

F
x
Y

S
x
T

D

D

A

A

V
x
N

K

I

A

K

G

N

F

V

I

L

W
|
W

D

D

A

E

Q

N

L
x
M

D

S

K

E

W
x
Y

L
|
L

A

T

D

N

P

P

R

K

G

K

F

Y

M

I

P

P

G

G

T
|
T

K
|
K

M
|
M

T

A

F
|
F

A

G

G

G

I

L

K

K

N

K

E

E

K

K

D

D

R

R

I

N

D

D

L

L

I

I

A
x
T

Y
|
Y

L

L

K

K

V
x
K

E

A

T

T

binding sulfonato-calix[8]arene: S7 (≠ D62), A8 (≠ L63), K9 (≠ S64), A12 (≠ Q67), T101 (≠ A155), Y102 (= Y156), K105 (≠ V159)
binding heme c: R18 (≠ L72), C19 (= C73), C22 (= C76), H23 (= H77), G34 (= G88), P35 (= P89), I40 (≠ V94), S45 (≠ A99), G46 (= G100), Y51 (= Y105), Y53 (≠ F107), T54 (≠ S108), N57 (≠ V111), W64 (= W118), M69 (≠ L123), Y72 (≠ W126), L73 (= L127), T83 (= T137), K84 (= K138), M85 (= M139), F87 (= F141)

6egzA Crystal structure of cytochromE C in complex with di-pegylated sulfonatocalix[4]arene (see paper)
46% identity, 63% coverage: 56:161/168 of query aligns to 1:107/108 of 6egzA

query
sites
6egzA

A

A

P

E

Y

F

N

K

T

A

G

G

D

S

L

A

S

K

N

K

G

G

Q

A

S

T

K

L

F

F

-

K

A

T

L

R

C
|
C

R

L

S

Q

C
|
C

H
|
H

T

T

I

V

T

E

E

K

G

G

P

P

D

H

L
x
K

T
x
V

G
|
G

P
|
P

N

N

L
|
L

Y

H

G

G

V
x
I

F

F

G

G

R

R

K

H

A
x
S

G

G

V

Q

K

A

E

E

G

G

Y
|
Y

S

S

F
x
Y

S
x
T

D

D

A

A

V
x
N

K

I

A

K

G

N

F

V

I

L

W

W

D

D

A

E

Q

N

L
x
M

D

S

K

E

W
x
Y

L
|
L

A

T

D

N

P

P

R

K

G

K

F

Y

M

I

P

P

G

G

T
|
T

K
|
K

M
|
M

T

A

F

F

A

G

G

G

I

L

K

K

N

K

E

E

K

K

D

D

R

R

I

N

D

D

L

L

I

I

A

T

Y

Y

L

L

K

K

V

K

E

A

T

T

binding di-PEGylated sulfonatocalix[4]arene: K32 (≠ L86), V33 (≠ T87)
binding heme c: C19 (= C73), C22 (= C76), H23 (= H77), G34 (= G88), P35 (= P89), L37 (= L91), I40 (≠ V94), S45 (≠ A99), Y51 (= Y105), Y53 (≠ F107), T54 (≠ S108), N57 (≠ V111), M69 (≠ L123), Y72 (≠ W126), L73 (= L127), T83 (= T137), K84 (= K138), M85 (= M139)

6egyA Crystal structure of cytochromE C in complex with mono-pegylated sulfonatocalix[4]arene (see paper)
46% identity, 63% coverage: 56:161/168 of query aligns to 1:107/108 of 6egyA

query
sites
6egyA

A

A

P

E

Y

F

N

K

T

A

G

G

D

S

L
x
A

S
x
K

N

K

G

G

Q
x
A

S

T

K

L

F

F

-

K

A

T

L
x
R

C
|
C

R

L

S
x
Q

C
|
C

H
|
H

T

T

I

V

T

E

E

K

G

G

P

P

D

H

L
x
K

T
x
V

G
|
G

P

P

N

N

L
|
L

Y

H

G

G

V
x
I

F

F

G

G

R

R

K

H

A
x
S

G

G

V

Q

K

A

E

E

G

G

Y
|
Y

S

S

F
x
Y

S
x
T

D

D

A

A

V
x
N

K

I

A

K

G

N

F

V

I

L

W
|
W

D

D

A

E

Q

N

L
x
M

D

S

K

E

W
x
Y

L
|
L

A

T

D

N

P

P

R

K

G

K

F

Y

M

I

P

P

G

G

T
|
T

K
|
K

M
|
M

T

A

F
|
F

A
x
G

G

G

I

L

K
|
K

N

K

E

E

K

K

D

D

R

R

I

N

D

D

L
|
L

I

I

A

T

Y
|
Y

L

L

K

K

V
x
K

E

A

T

T

binding mono-PEGylated sulfonatocalix[4]arene: A8 (≠ L63), K9 (≠ S64), A12 (≠ Q67), R18 (≠ L72), Q21 (≠ S75), K32 (≠ L86), V33 (≠ T87), F87 (= F141), G88 (≠ A142), K91 (= K145), Y102 (= Y156), K105 (≠ V159)
binding heme c: C19 (= C73), C22 (= C76), H23 (= H77), G34 (= G88), L37 (= L91), I40 (≠ V94), S45 (≠ A99), Y51 (= Y105), Y53 (≠ F107), T54 (≠ S108), N57 (≠ V111), W64 (= W118), M69 (≠ L123), Y72 (≠ W126), L73 (= L127), T83 (= T137), K84 (= K138), M85 (= M139), L99 (= L153)

5lftA Crystal structure of cytochromE C - bromo-trisulfonatocalix[4]arene complexes (see paper)
46% identity, 63% coverage: 56:161/168 of query aligns to 1:107/108 of 5lftA

query
sites
5lftA

A

A

P

E

Y

F

N

K

T

A

G

G

D
x
S

L

A

S

K

N

K

G

G

Q

A

S

T

K

L

F

F

-

K

A

T

L

R

C
|
C

R

L

S

Q

C
|
C

H
|
H

T

T

I

V

T

E

E

K

G

G

P

P

D

H

L

K

T

V

G
|
G

P
|
P

N

N

L

L

Y

H

G

G

V
x
I

F

F

G

G

R

R

K

H

A
x
S

G

G

V

Q

K

A

E

E

G

G

Y

Y

S

S

F
x
Y

S
x
T

D

D

A

A

V
x
N

K

I

A

K

G

N

F

V

I

L

W

W

D

D

A

E

Q

N

L
x
M

D

S

K

E

W
x
Y

L
|
L

A

T

D

N

P

P

R

K

G

K

F

Y

M

I

P

P

G

G

T
|
T

K
|
K

M
|
M

T

A

F

F

A

G

G

G

I
x
L

K
|
K

N

K

E

E

K
|
K

D

D

R

R

I

N

D

D

L

L

I

I

A

T

Y

Y

L

L

K

K

V

K

E

A

T

T

binding Bromo-trisulfonatocalix[4]arene: S7 (≠ D62), L90 (≠ I144), K91 (= K145), K94 (= K148)
binding heme c: C19 (= C73), C22 (= C76), H23 (= H77), G34 (= G88), P35 (= P89), I40 (≠ V94), S45 (≠ A99), Y53 (≠ F107), T54 (≠ S108), N57 (≠ V111), M69 (≠ L123), Y72 (≠ W126), L73 (= L127), T83 (= T137), K84 (= K138), M85 (= M139)

5kpfB Crystal structure of cytochromE C - phenyl-trisulfonatocalix[4]arene complex (see paper)
46% identity, 63% coverage: 56:161/168 of query aligns to 1:107/108 of 5kpfB

query
sites
5kpfB

A

A

P

E

Y

F

N

K

T

A

G

G

D
x
S

L
x
A

S
x
K

N
x
K

G

G

Q

A

S

T

K

L

F

F

-

K

A

T

L
x
R

C
|
C

R

L

S

Q

C
|
C

H
|
H

T

T

I

V

T

E

E

K

G

G

P

P

D

H

L

K

T
x
V

G
|
G

P
|
P

N

N

L

L

Y

H

G

G

V
x
I

F

F

G

G

R

R

K

H

A
x
S

G

G

V

Q

K

A

E

E

G

G

Y

Y

S

S

F
x
Y

S
x
T

D

D

A

A

V
x
N

K

I

A

K

G

N

F

V

I

L

W
|
W

D

D

A

E

Q

N

L
x
M

D

S

K

E

W
x
Y

L
|
L

A

T

D

N

P

P

R

K

G

K

F

Y

M

I

P

P

G

G

T
|
T

K
|
K

M
|
M

T

A

F
|
F

A

G

G

G

I

L

K

K

N

K

E

E

K

K

D

D

R

R

I

N

D

D

L

L

I

I

A

T

Y

Y

L

L

K

K

V

K

E

A

T

T

binding Phenyl-trisulfonatocalix[4]arene: S7 (≠ D62), A8 (≠ L63), K9 (≠ S64), K10 (≠ N65)
binding heme c: R18 (≠ L72), C19 (= C73), C22 (= C76), H23 (= H77), V33 (≠ T87), G34 (= G88), P35 (= P89), I40 (≠ V94), S45 (≠ A99), Y53 (≠ F107), T54 (≠ S108), N57 (≠ V111), W64 (= W118), M69 (≠ L123), Y72 (≠ W126), L73 (= L127), T83 (= T137), K84 (= K138), M85 (= M139), F87 (= F141)

P00042 Cytochrome c from Wickerhamomyces anomalus (Yeast) (Hansenula anomala) (see paper)
46% identity, 61% coverage: 55:157/168 of query aligns to 1:104/109 of P00042

query
sites
P00042

P

P

A

A

P

P

Y

F

N

K

T

K

G

G

D

S

L

E

S

K

N

K

G

G

Q

A

S

T

K

L

F

F

-

K

A

T

L

R

C
|
C

R

L

S

Q

C
|
C

H

H

T

T

I

V

T

E

E

K

G

G

P

P

D

H

L

K

T

V

G

G

P

P

N

N

L

L

Y

H

G

G

V

I

F

F

G

G

R

R

K

Q

A

S

G

G

V

K

K

A

E

E

G

G

Y

Y

S

S

F

Y

S

T

D

D

A

A

V

N

K

I

A

K

A
x
K

G

A

F

V

I

E

W

W

D

S

A

E

Q

Q

Q

T

L

M

D

S

K

D

W

Y

L

L

A

E

D

N

P

P

R
x
K

G
x
K

F

Y

M

I

P

P

G

G

T

T

K

K

M

M

T

A

F

F

A

G

G

G

I

L

K

K

N

K

E

E

K

K

D

D

R

R

I

N

D

D

L

L

I

V

A

T

Y

Y

L

L

C20 (= C73) binding covalent
C23 (= C76) binding covalent
K61 (≠ A114) modified: N6,N6-dimethyllysine; alternate; modified: N6-methyllysine; alternate
K78 (≠ R131) modified: N6,N6,N6-trimethyllysine
K79 (≠ G132) modified: N6,N6,N6-trimethyllysine

Query Sequence

>CCNA_01268 FitnessBrowser__Caulo:CCNA_01268
MRSLNLAAVAALCVIVSACSKDGGSSSQAPADAPTPAAAPAPTDAEKAAALAALPAPYNT
GDLSNGQSKFALCRSCHTITEGGPDLTGPNLYGVFGRKAGVKEGYSFSDAVKAAGFIWDA
QQLDKWLADPRGFMPGTKMTFAGIKNEKDRIDLIAYLKVETGYQTPAK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory