SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing CCNA_01356 FitnessBrowser__Caulo:CCNA_01356 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

5eynA Crystal structure of fructokinase from vibrio cholerae o395 in fructose, adp, beryllium trifluoride and calcium ion bound form
28% identity, 46% coverage: 35:332/642 of query aligns to 22:305/306 of 5eynA

query
sites
5eynA

F

Y

A

L

K

K

Y

C

L

P

G
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G

S
x
A

P

P

T

A

N
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N

T

V

A

A

A

V

G

A

G

I

A

A

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L

L

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L

G

K

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L

F

T

G

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V

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G

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N

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D

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F

G

G

R

R

F

F

I

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G

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V

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x
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A

-

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-

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F

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I

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A

A

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K

A

E

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G

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Y

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V

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F

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R

P

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-

D

-

A

-

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-

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N

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N

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Q

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T

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A

E

A

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A

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I

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A

R

D

A

F

S

Q

D

I

A

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L

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L

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V

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C

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K
x
T

R

L

G
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G

P
x
A

E

K

G
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G

C

A

V

L

A

V

F

-

P

-

G

-

A

A

I

T

P

P

D

N

A

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L

-

D

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E

Q

G

I

V

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S

S

A

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R

K

G
x
A

F

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K

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V
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V

E

K

V
x
P

F

I

N

D

V
x
T

L
x
T

G
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G

A
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A

G
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G

D
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D

A

A

F
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F

M

V

A

G

G

G

F

L

L

L

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Y

-

R

-

L

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W

L

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N

H

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E

L

T

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C

A

C

V

E

K

W

W

G

A

N
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N

A

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C

C

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G

A
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A

I

L

V

A

V

T

S

T

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Q

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K

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G

C

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M

P

T

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M

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W

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L

active site: G246 (= G279), A247 (= A280), G248 (= G281), D249 (= D282)
binding adenosine-5'-diphosphate: H91 (≠ R104), T217 (≠ K244), G219 (= G246), A220 (≠ P247), G222 (= G249), A238 (≠ G269), V239 (= V272), P241 (≠ V274), T244 (≠ V277), G246 (= G279), A247 (= A280), G248 (= G281), F251 (= F284), N279 (= N306), G282 (= G309), A283 (= A310)
binding beryllium trifluoride ion: N157 (≠ D172), R159 (= R174), T245 (≠ L278), G246 (= G279), A247 (= A280), G248 (= G281), D249 (= D282)
binding beta-D-fructofuranose: G27 (= G40), G28 (= G41), A29 (≠ S42), N32 (= N45), V84 (= V97), F96 (≠ L109), F98 (= F111), I127 (≠ T142), N157 (≠ D172), R159 (= R174), G246 (= G279), D249 (= D282)

Sites not aligning to the query:

binding beta-D-fructofuranose: 9, 11, 13

5yggA Crystal structure of fructokinase double-mutant (t261c-h108c) from vibrio cholerae o395 in fructose, adp and potassium ion bound form (see paper)
28% identity, 46% coverage: 35:332/642 of query aligns to 26:309/310 of 5yggA

query
sites
5yggA

F

Y

A

L

K

K

Y

C

L

P

G
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G

S
x
A

P

P

T

A

N
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N

T

V

A

A

A

V

G

A

G

I

A

A

R

R

L

L

G

S

L

G

K

R

T

S

G

A

L

F

T

G

R

R

V

V

G

G

A

N

D

D

H

P

M

F

G

G

R

R

F

F

I

M

R

Q

E

Q

Q

T

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L

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D

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E

G

Q

V

V

D

D

V

C

A

Q

G

H

V

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L

H

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F

D

D

P

P

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R

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I

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G

D

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x
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F

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D

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N

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binding adenosine-5'-diphosphate: K188 (≠ V211), S190 (≠ T213), T221 (≠ K244), G223 (= G246), A224 (≠ P247), G226 (= G249), K241 (≠ R268), A242 (≠ G269), V243 (= V272), A251 (= A280), G252 (= G281), F255 (= F284), N283 (= N306), G286 (= G309), A287 (= A310)
binding beta-D-fructofuranose: G31 (= G40), G32 (= G41), A33 (≠ S42), N36 (= N45), V88 (= V97), F100 (≠ L109), F102 (= F111), I131 (≠ T142), N161 (≠ D172), R163 (= R174), D253 (= D282)

Sites not aligning to the query:

binding beta-D-fructofuranose: 13, 15, 17

1v1aA 2-keto-3-deoxygluconate kinase from thermus thermophilus with bound 2- keto-3-deoxygluconate and adp (see paper)
30% identity, 51% coverage: 8:332/642 of query aligns to 2:301/301 of 1v1aA

query
sites
1v1aA

L

L

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E

L

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A

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A

A

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A

G

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A

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L

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G

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A
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D
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M

A

A

A

G

G

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Y

L

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A

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x
A

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x
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L

active site: G248 (= G279), A249 (= A280), G250 (= G281), D251 (= D282)
binding adenosine-5'-diphosphate: K219 (= K244), G221 (= G246), A222 (≠ P247), F239 (= F270), V241 (= V272), A243 (≠ V274), A249 (= A280), G250 (= G281), F253 (= F284), N275 (= N306), G278 (= G309), A279 (= A310), A282 (≠ V313)
binding 2-keto-3-deoxygluconate: L11 (≠ S17), G33 (= G40), G34 (= G41), A35 (≠ S42), N38 (= N45), Y89 (≠ R102), Y103 (≠ F111), R105 (= R113), I134 (vs. gap), R167 (= R174), G248 (= G279), D251 (= D282), D287 (≠ C318)

Q53W83 2-dehydro-3-deoxygluconokinase; 2-keto-3-deoxygluconokinase; 3-deoxy-2-oxo-D-gluconate kinase; KDG kinase; EC 2.7.1.45 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) (see paper)
30% identity, 51% coverage: 8:333/642 of query aligns to 2:302/309 of Q53W83

query
sites
Q53W83

L

L

D

E

L

V

I

V

A

T

V

A

G

G

R

E

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S

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Y

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G
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G

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x
A

P
x
E

T
x
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N
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T

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A

A

A

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G

A

G

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A

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R

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L

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-

G

-

E

-

N

-

R

-

F

-

V

-

E

S

N

P

Q

E

V

A

T

R

A

G

K

F

L

L

Q

E

E

R

V

A

V

L

A

P

L

G

C

V

D

D

L

L

I

L

V

F

G

L

T
x
S

E

E

I

A

H

E

I

L

L

L

G

F

G

G

S

R

T

V

D

E

T

E

I

-

A

-

A

A

L

L

R

R

A

A

I

L

R

-

A

-

S

S

D

A

A

P

L

E

L

V

V

V

C

L

K
|
K

R
|
R

G
|
G

P
x
A

E
x
K

G
|
G

C
x
A

V

W

A

A

F

F

P

V

G

D

A

G

I

-

P

-

D

-

A

-

L

-

D

-

E

R

G

R

V

V

S

E

A

G

R

S

G

A

F

F

K

A

V

V

E

E

V

A

F

V

N

D

V

P

L

V

G
|
G

A
|
A

G
|
G

D
|
D

A

A

F

F

M

A

A

A

G

G

F

Y

L

L

R

A

G

G

W

A

L

V

R

W

H

G

E

L

S

P

V

V

E

E

T

E

C

R

C

L

E

R

W

L

G

A

N
|
N

A

L

C

L

G

G

A

A

I

S

V

V

V

A

S

A

R

S

H

R

G

G

C
x
D

T

H

P

E

A

G

M

A

P

P

T

Y

W

R

I

E

E

D

L

L

Q

E

A

V

F

L

L

L

S

K

GAEVN 34:38 (≠ GSPTN 41:45) binding
YYR 103:105 (≠ FYR 111:113) binding
R167 (= R174) binding
S193 (≠ T213) binding
219:225 (vs. 244:250, 57% identical) binding
GAGD 248:251 (= GAGD 279:282) binding
D251 (= D282) binding
N275 (= N306) binding
D287 (≠ C318) binding

1v1bA 2-keto-3-deoxygluconate kinase from thermus thermophilus with bound atp (see paper)
30% identity, 50% coverage: 8:329/642 of query aligns to 2:298/300 of 1v1bA

query
sites
1v1bA

L

L

D

E

L

V

I

V

A

T

V

A

G

G

R

E

S

P

S

L

V

V

D

A

L

L

Y

V

G

P

Q

Q

Q

E

V

P

G

G

G

-

R

H

L

L

E

R

D

G

M

K

G

R

S

L

F

L

A

E

K

V

Y

Y

L

V

G

G

S

A

P

E

T

V

N

N

T

V

A

A

A

V

G

A

G

L

A

A

R

R

L

L

G

G

L

V

K

K

T

V

G

G

L

F

L

V

T

G

R

R

V

V

G

G

A

E

D

D

H

E

M

L

G

G

R

A

F

M

I

V

R

E

E

E

Q

R

L

L

E

R

R

A

E

E

G

G

V

V

D

D

V

L

A

T

G

H

V

F

L

R

S

R

D

A

P

P

D

G

R

-

L

-

T

-

A

-

L

-

V

-

I

F

L

T

G

G

I

L

R

Y

D

L

R

R

V

E

N

Y

F

L

P

P

L

L

-

G

-

Q

-

G

-

R

-

V

I

F

F

Y

Y

Y

R

R

E

K

N

G

C

S

A

A

D

G

M

S

A

A

L

L

E

A

P

P

S

G

D

A

V

F

D

D

E

P

A

D

W

Y

F

L

A

E

Q

G

A

V

G

R

A

F

V

L

L

H

I

L

N

S

G

G

-

I

T

T

H

P

L

A

S

L

Q

S

P

P

N

E

V

A

Y

R

E

A

T

F

S

S

L

L

K

W

A

A

A

M

R

E

A

E

V

A

K

K

A

R

G

G

G

V

R

R

V

V

A

S

F

L

D

D

I

V

D
x
N

Y

Y

R

R

P

Q

V

T

L

L

W

W

G

-

L

-

T

-

G

-

K

-

D

-

A

-

G

-

E

-

N

-

R

-

F

-

V

-

E

S

N

P

Q

E

V

A

T

R

A

G

K

F

L

L

Q

E

E

R

V

A

V

L

A

P

L

G

C

V

D

D

L

L

I

L

V

F

G

L

T
x
S

E

E

I

A

H

E

I

L

L

L

G

F

G

G

S

R

T

V

D

E

T

E

I

-

A

-

A

A

L

L

R

R

A

A

I

L

R

-

A

-

S

S

D

A

A

P

L

E

L

V

V

V

C

L

K
|
K

R

R

G
|
G

P

A

E

K

G
|
G

C

A

V

W

A

A

F

F

P

V

G

D

A

G

I

-

P

-

D

-

A

-

L

-

D

-

E

R

G

R

V

V

S

E

A

G

R

S

G
x
A

F
|
F

K

A

V
|
V

E

E

V

A

F

V

N

D

V
x
P

L
x
V

G
|
G

A
|
A

G
|
G

D
|
D

A

A

F
|
F

M

A

A

A

G

G

F

Y

L

L

R

A

G

G

W

A

L

V

R

W

H

G

E

L

S

P

V

V

E

E

T

E

C

R

C

L

E

R

W

L

G

A

N
|
N

A

L

C

L

G
|
G

A
|
A

I

S

V

V

V
x
A

S

A

R

S

H

R

G

G

C

D

T

H

P

E

A

G

M

A

P

P

T

Y

W

R

I

E

E

D

L

L

Q

E

active site: G248 (= G279), A249 (= A280), G250 (= G281), D251 (= D282)
binding adenosine-5'-triphosphate: N165 (≠ D172), S193 (≠ T213), K219 (= K244), G221 (= G246), G224 (= G249), A238 (≠ G269), F239 (= F270), V241 (= V272), P246 (≠ V277), V247 (≠ L278), G248 (= G279), A249 (= A280), G250 (= G281), D251 (= D282), F253 (= F284), N275 (= N306), G278 (= G309), A279 (= A310), A282 (≠ V313)

3gbuA Crystal structure of an uncharacterized sugar kinase ph1459 from pyrococcus horikoshii in complex with atp
26% identity, 44% coverage: 12:291/642 of query aligns to 4:254/302 of 3gbuA

query
sites
3gbuA

A

S

V

I

G

G

R

E

S

L

V

I

D

D

L

L

Y

I

G

S

Q

V

Q

E

V

-

G

E

G

G

R

D

L

L

E

K

D

D

M

V

G

R

S

L

F

F

A

E

K

K

Y

H

L

P

G

G

S

A

P

P

T

A

N

N

T

V

A

A

A

V

G

G

G

V

A

S

R

R

L

L

G

G

L

V

K

K

T

S

G

S

L

L

L

I

T

S

R

K

V

V

G

G

A

N

D

D

H

P

M

F

G

G

R

E

F

Y

I

L

R

I

E

E

Q

E

L

L

E

S

R

K

E

E

G

N

V

V

D

D

V

T

A

R

G

G

V

I

L

V

S

K

D

D

P

E

D

K

R

K

L

H

T

T

A

G

L

I

V

V

I

F

L

V

G

Q

I

L

R

K

D

G

R

A

V

S

N

-

F

-

P

P

L

S

I

F

F

L

Y

L

R

Y

E

D

N

D

C

V

A

A

D

Y

M

F

A

N

L

M

E

T

P

L

S

N

D

D

V

I

D

N

E

W

A

D

W

I

F

V

A

E

Q

E

A

A

G

K

A

I

V

V

L

N

I

F

N

G

G

S

T

V

H

I

L

L

S

A

Q

R

P

N

N

P

V

S

Y

R

E

E

T

T

S

V

L

M

K

K

A

V

A

I

R

K

A

K

V

I

K

K

A

G

A

S

G

-

G

S

R

L

V

I

A

A

F

F

D

D

I

V

D

N

Y

L

R

R

P

L

V

D

L

L

W

W

G

-

L

-

T

-

G

-

K

-

D

-

A

-

G

-

E

-

N

-

R

R

F

G

V

Q

E

E

N

E

Q

E

Q

M

V

I

T

K

A

V

K

-

L

L

Q

E

E

E

V

S

V

I

A

K

L

L

C

A

D

D

L

I

I

V

V
x
K

G

A

T

S

E

E

I

V

H

L

I

Y

L

L

G

E

G

N

S

-

T

-

D

-

T

-

I

-

A

-

L

-

R

Q

A

G

I

V

R

E

R

V

A

K

S

G

D

S

A

M

L

L

L

T

V

A

C

I

K
x
T

R

L

G
|
G

P
|
P

E

K

G
|
G

C
x
F

V

R

A

L

F

I

P

K

G

N

A

-

I

-

P

-

D

-

A

-

L

-

D

E

E

T

G

V

V

V

S

D

A

V

R

P

G
x
S

F

Y

K

N

V
|
V

E

N

V
x
P

F

L

N

D

V
x
T

L

T

G
|
G

A
|
A

G
|
G

D
|
D

A

A

F

F

M

M

A

A

G

A

F

L

L

L

R

V

G

G

active site: G242 (= G279), A243 (= A280), G244 (= G281), D245 (= D282)
binding adenosine-5'-triphosphate: K188 (≠ V211), T213 (≠ K244), G215 (= G246), P216 (= P247), G218 (= G249), F219 (≠ C250), S232 (≠ G269), V235 (= V272), P237 (≠ V274), T240 (≠ V277), A243 (= A280), G244 (= G281)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 274, 278

3ih0A Crystal structure of an uncharacterized sugar kinase ph1459 from pyrococcus horikoshii in complex with amp-pnp
26% identity, 44% coverage: 12:291/642 of query aligns to 5:255/304 of 3ih0A

query
sites
3ih0A

A

S

V

I

G

G

R

E

S

L

V

I

D

D

L

L

Y

I

G

S

Q

V

Q

E

V

-

G

E

G

G

R

D

L

L

E

K

D

D

M

V

G

R

S

L

F

F

A

E

K

K

Y

H

L

P

G

G

S

A

P

P

T

A

N

N

T

V

A

A

A

V

G

G

G

V

A

S

R

R

L

L

G

G

L

V

K

K

T

S

G

S

L

L

L

I

T

S

R

K

V

V

G

G

A

N

D

D

H

P

M

F

G

G

R

E

F

Y

I

L

R

I

E

E

Q

E

L

L

E

S

R

K

E

E

G

N

V

V

D

D

V

T

A

R

G

G

V

I

L

V

S

K

D

D

P

E

D

K

R

K

L

H

T

T

A

G

L

I

V

V

I

F

L

V

G

Q

I

L

R

K

D

G

R

A

V

S

N

-

F

-

P

P

L

S

I

F

F

L

Y

L

R

Y

E

D

N

D

C

V

A

A

D

Y

M

F

A

N

L

M

E

T

P

L

S

N

D

D

V

I

D

N

E

W

A

D

W

I

F

V

A

E

Q

E

A

A

G

K

A

I

V

V

L

N

I

F

N

G

G

S

T

V

H

I

L

L

S

A

Q

R

P

N

N

P

V

S

Y

R

E

E

T

T

S

V

L

M

K

K

A

V

A

I

R

K

A

K

V

I

K

K

A

G

A

S

G

-

G

S

R

L

V

I

A

A

F

F

D

D

I

V

D

N

Y

L

R

R

P

L

V

D

L

L

W

W

G

-

L

-

T

-

G

-

K

-

D

-

A

-

G

-

E

-

N

-

R

R

F

G

V

Q

E

E

N

E

Q

E

Q

M

V

I

T

K

A

V

K

-

L

L

Q

E

E

E

V

S

V

I

A

K

L

L

C

A

D

D

L

I

I

V

V
x
K

G

A

T

S

E

E

I

V

H

L

I

Y

L

L

G

E

G

N

S

-

T

-

D

-

T

-

I

-

A

-

L

-

R

Q

A

G

I

V

R

E

R

V

A

K

S

G

D

S

A

M

L

L

L

T

V

A

C

I

K
x
T

R

L

G
|
G

P
|
P

E

K

G
|
G

C
x
F

V

R

A

L

F

I

P

K

G

N

A

-

I

-

P

-

D

-

A

-

L

-

D

E

E

T

G

V

V

V

S

D

A

V

R

P

G
x
S

F

Y

K

N

V
|
V

E

N

V
x
P

F

L

N

D

V
x
T

L

T

G
|
G

A
|
A

G
|
G

D
|
D

A

A

F

F

M

M

A

A

G

A

F

L

L

L

R

V

G

G

active site: G243 (= G279), A244 (= A280), G245 (= G281), D246 (= D282)
binding phosphoaminophosphonic acid-adenylate ester: K189 (≠ V211), T214 (≠ K244), G216 (= G246), P217 (= P247), G219 (= G249), F220 (≠ C250), S233 (≠ G269), V236 (= V272), P238 (≠ V274), T241 (≠ V277), A244 (= A280), G245 (= G281)

Sites not aligning to the query:

binding phosphoaminophosphonic acid-adenylate ester: 275, 279

Q8ZKR2 Aminoimidazole riboside kinase; AIRs kinase; EC 2.7.1.223 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
27% identity, 50% coverage: 13:336/642 of query aligns to 10:311/319 of Q8ZKR2

query
sites
Q8ZKR2

V

I

G

G

R

D

S

A

S

S

V

V

D
|
D

L

L

Y

V

G

P

Q

-

V

-

G

-

R

-

L

-

E

E

D

K

M

Q

G

N

S

S

F

Y

A

L

K

K

Y

C

L

P

G

G

G
|
G

S

A

P

S

T

A

N

N

T

V

A

G

A

V

G

C

G

V

A

A

R

R

L

L

G

G

L

G

K

E

T

C

G

G

L

F

L

I

T

G

R

C

V

L

G

G

A

D

D

D

H

D

M

A

G

G

R

R

F

F

I

L

R

R

E

Q

Q

V

L

F

E

Q

R

D

E

N

G

G

V

V

D

D

V

V

A

T

G

F

V

L

L

R

S

L

D

D

P

A

D

D

R

L

L

T

T

S

A

A

L

V

V

L

I

I

L

V

G

N

I

L

R

T

D

A

R

D

V

G

N

E

F

R

P

S

L

F

I

T

F
x
Y

Y

L

R

V

E

H

N

P

C

G

A

A

D

D

M

T

A

Y

L

V

E

S

P

P

S

Q

D

D

V

L

D

P

E

P

A

-

W

-

F

F

A

R

Q

Q

A

Y

G

E

A

W

V

F

L

Y

I

F

N

S

G

S

T

I

H

G

L

L

S

T

Q

D

P

R

N

P

V

A

Y

R

E

E

T

A

S

C

L

L

K

E

A

G

A

A

R

R

A

R

V

M

K

R

A

E

A

A

G

G

R

Y

V

V

A

L

F

F

D

D

I

V

D

N

Y

L

R
|
R

P

S

V

K

L

M

W

W

G

G

L

N

T

T

G

D

K

-

D

-

A

-

G

-

E

-

N

-

R

-

F

-

V

-

E

E

N

I

Q

P

Q

E

V

L

T

I

A

A

K

R

L

S

Q
x
A

E
x
A

V

L

V
x
A

A

S

L

I

C

C

D

K

L

V

I

-

V

-

G

-

T

S

E

A

E

D

E

E

I

L

H

C

I

Q

L

L

G

S

G

G

S

A

T

S

D

H

T

W

I

Q

A

D

A

A

L

R

R

Y

A

Y

I

L

R

R

R

D

-

L

-
x
G

-

C

-

D

-

T

-

I

-

S

-

L

-

G

A

A

S

D

D

G

A

A

L

L

V

I

C

T

K

A

R

-

G

-

P

-

E

E

G

G

C

E

V

F

A

H

F

F

P

P

G

A

A

P

I

-

P

-

D

-

A

-

L

-

D

-

E

-

G

-

V

-

S

-

A

-

R

-

G

-

F

-

K

R

V

V

E

D

V

V

F

V

N
x
D

V

T

L
x
T

G

G

A

A

G

G

D
|
D

A

A

F

F

M

V

A

G

G

G

F

L

L

L

R

F

G

T

W

L

L

S

R

R

-

A

-

N

-

C

-

W

-

D

H

H

E

A

S

L

V

L

E

A

T

E

C

A

C

I

E

S

W

N

G

A

N

N

A

A

C

C

G

G

A

A

I

M

V
x
A

V

V

S

T

R
x
A

H

K

G
|
G

C

A

T

M

P

T

A

A

M

L

P

P

T

F

W

P

I

D

E

Q

L

L

Q

N

A

T

F

F

L

L

S

S

E

S

R

H

E

S

D16 (= D19) binding
G31 (= G41) binding
Y101 (≠ F111) binding
R162 (= R174) binding
A180 (≠ Q201) binding
A181 (≠ E202) binding
A183 (≠ V204) binding
G213 (vs. gap) binding
D246 (≠ N276) binding
T248 (≠ L278) binding
D252 (= D282) binding
A287 (≠ V312) binding
A290 (≠ R315) binding
G292 (= G317) binding

2dcnA Crystal structure of 2-keto-3-deoxygluconate kinase from sulfolobus tokodaii complexed with 2-keto-6-phosphogluconate (alpha-furanose form)
28% identity, 48% coverage: 27:334/642 of query aligns to 19:307/308 of 2dcnA

query
sites
2dcnA

G

G

R

P

L

L

E

R

D

H

M

V

G

S

S

Y

F

F

A

E

K

K

Y

H

L

V

G
x
A

G
|
G

S
|
S

P

E

T

A

N
|
N

T

Y

A

C

A

V

G

A

G

F

A

I

R

K

L

Q

G

G

L

N

K

E

T

C

G

G

L

I

T

A

R

K

V

V

G

G

A

D

D

D

H

E

M

F

G

G

R

Y

F

N

I

A

R

I

E

E

Q

W

L

L

E

R

R

G

E

Q

G

G

V

V

D

D

V

V

A

S

G

H

V

M

L

K

S

I

D

D

P

P

D

S

R

A

L

P

T

T

A

G

L

-

V

-

I

I

L
x
F

G

F

I

I

R

Q

D

R

R

H

V

Y

N

P

F

V

P

P

L

L

-

K

-

S

-

E

-

S

I

I

F
x
Y

Y

Y

R
|
R

E

K

N

G

C

S

A

A

D

G

M

S

A

K

L

L

E

S

P

P

S

E

D

D

V

V

D

D

E

E

A

E

W

Y

F

V

A

K

Q

S

A

A

G

D

A

L

V

V

L

H

I

S

N

S

G

G

T
x
I

H

T

L

L

S

A

Q

I

P

S

N

S

V

T

Y

A

E

K

T

E

S

A

L

V

K

-

A

-

A

Y

R

K

A

A

V

F

K

E

A

I

A

A

G

S

G

N

R

R

V

-

A

S

F

F

D

D

I

T

D

N

Y

I

R
|
R

P

L

V

K

L

L

W

W

G

S

L

A

T

E

G

-

K

-

D

-

A

-

G

-

E

-

N

-

R

-

F

-

V

-

E

E

N

A

Q

K

Q

R

V

E

T

I

A

L

K

K

L

L

Q

L

E

S

V

K

V

F

A

H

L

L

C

K

D

F

L

L

I

I

V

T

G
x
D

T

T

E

D

E

S

I

K

H

I

I

I

L

L

G

G

G

E

S

S

T

-

D

D

T

P

I

D

A

K

A

A

L

A

R

K

A

A

I

F

R

S

R

D

A

Y

S

A

D

E

A

-

L

I

V

V

C

M

K
|
K

R
x
L

G
|
G

P
|
P

E

K

G
|
G

C

A

V

I

A

V

F

Y

P

Y

G

D

A

G

I

-

P

-

D

-

A

-

L

-

D

-

E

K

G

K

V

Y

S

Y

A

S

R

S

G

G

F
x
Y

K

Q

V

V

E

P

V
|
V

F

E

N

D

V
|
V

L
x
T

G
|
G

A
|
A

G
|
G

D
|
D

A

A

F
x
L

M

G

A

G

G

T

F

F

L

L

R

S

G

L

W

Y

L

Y

R

K

H

G

E

F

S

E

V

M

E

E

T

K

C

A

C

L

E

D

W

Y

G

A

N
x
I

A

V

C

A

G
x
S

A

T

I

L

V

N

V
|
V

S

M

R

I

H

R

G

G

C
x
D

T

Q

P

E

A

N

M

L

P

P

T

T

W

T

I

K

E

D

L

I

Q

E

A

T

F

F

L

L

S

R

E

E

active site: G252 (= G279), A253 (= A280), G254 (= G281), D255 (= D282)
binding adenosine-5'-diphosphate: D193 (≠ G212), K223 (= K244), L224 (≠ R245), G225 (= G246), P226 (= P247), G228 (= G249), Y243 (≠ F270), V247 (= V274), V250 (= V277), A253 (= A280), G254 (= G281), L257 (≠ F284), I279 (≠ N306), S282 (≠ G309), V286 (= V313)
binding 6-O-phosphono-beta-D-psicofuranosonic acid: A32 (≠ G40), G33 (= G41), S34 (= S42), N37 (= N45), F89 (≠ L99), Y105 (≠ F111), R107 (= R113), I136 (≠ T142), R165 (= R174), T251 (≠ L278), G252 (= G279), A253 (= A280), G254 (= G281), D255 (= D282), D291 (≠ C318)

Sites not aligning to the query:

binding 6-O-phosphono-beta-D-psicofuranosonic acid: 10

2varA Crystal structure of sulfolobus solfataricus 2-keto-3-deoxygluconate kinase complexed with 2-keto-3-deoxygluconate (see paper)
25% identity, 51% coverage: 8:334/642 of query aligns to 1:309/311 of 2varA

query
sites
2varA

L

V

D

D

L

V

I

I

A

A

V

L

G

G

R

E

S

P

S
x
L

V

I

D

Q

L

-

Y

F

G

N

Q

S

Q

F

V

N

G

P

G

G

R

P

L

L

E

R

D

F

M

V

G

N

S

Y

F

F

A

E

K

K

Y

H

L

V

G
x
A

G
|
G

S
|
S

P

E

T

L

N
|
N

T

F

A

C

A

I

G

A

G

V

A

V

R

R

L

N

G

H

L

L

K

S

T

C

G

S

L

L

L

I

T

A

R

R

V

V

G

G

A

N

D

D

H

E

M

F

G

G

R

K

F

N

I

I

R

I

E

E

Q

Y

L

S

E

R

R

A

E

Q

G

G

V

I

D

D

V

T

A

S

G

H

V

I

L

K

S

V

D

D

P

N

D

E

R

S

L

F

T

T

A

G

L

I

V
x
Y

I

F

L

I

-

Q

-

R

G

G

I

Y

R

P

D

I

R

P

V

M

N

K

F

S

P

E

L
|
L

I

V

F
x
Y

Y

Y

R
|
R

E

K

N

G

C

S

A

A

D

G

M

S

A

R

L

L

E

S

P

P

S

E

D

D

V

I

D

N

E

E

A

N

W

Y

F

V

A

R

Q

N

A

S

G

R

A

L

V

V

L

H

I

S

N

T

G

G

T
x
I

H

T

L

L

S

A

-

I

Q

S

P

D

N

N

V

A

Y

K

E

E

T

A

S

V

L

I

K

K

A

A

A

F

R

E

A

L

V

A

K

K

A

S

A

R

G

-

R

-

V

-

A

S

F

L

D

D

I

T

D
x
N

Y

I

R
|
R

P

P

V

K

L

L

W

W

G

S

L

S

T

L

G

E

K

K

D

-

A

-

G

-

E

-

N

-

R

-

F

-

V

-

E

A

N

K

Q

E

Q

T

V

I

T

L

A

S

K

I

L

L

Q

K

E

K

V

Y

V

D

A

-

L

-

C

I

D

E

L

V

I

L

V

I

G

T

T

D

E

P

E

D

I

T

H

K

I

I

L

L

G

L

G

D

S

V

T

T

D

D

T

P

I

D

A

E

A

A

L

Y

R

R

A

K

I

Y

R

K

R

E

A

L

S

G

D

V

A

K

L

V

L

L

V

L

C

Y

K
|
K

R

L

G
|
G

P
x
S

E

K

G
|
G

C
x
A

V

I

A

A

F

Y

P

K

G

D

A

N

I

V

P

K

D

A

A

F

L

K

D

D

E

-

G

-

V

-

S

-

A

-

R

-

G
x
A

F
x
Y

K

K

V

V

E

P

V
|
V

F

E

N

D

V

P

L
x
T

G
|
G

A
|
A

G
|
G

D
|
D

A

A

F
x
M

M

A

A

G

G

T

F

F

L

V

R

S

G

L

W

Y

L

L

R

Q

H

G

E

K

S

D

V

I

E

E

T

Y

C

S

C

L

E

A

W

H

G

G

N
x
I

A

A

C

A

G
x
S

A

T

I

L

V

V

V
x
I

S

T

R

V

H

R

G

G

C
x
D

T

N

P

E

A

L

M

T

P

P

T

T

W

L

I

E

E

D

L

A

Q

E

A

R

F

F

L

L

S

N

E

E

active site: G254 (= G279), A255 (= A280), G256 (= G281), D257 (= D282)
binding adenosine monophosphate: K225 (= K244), G227 (= G246), S228 (≠ P247), G230 (= G249), A231 (≠ C250), A244 (≠ G269), Y245 (≠ F270), V249 (= V274), A255 (= A280), G256 (= G281), M259 (≠ F284), I281 (≠ N306), S284 (≠ G309), I288 (≠ V313)
binding phosphoaminophosphonic acid-adenylate ester: N163 (≠ D172), R165 (= R174), K225 (= K244), G227 (= G246), S228 (≠ P247), G230 (= G249), A231 (≠ C250), A244 (≠ G269), Y245 (≠ F270), V249 (= V274), T253 (≠ L278), G254 (= G279), A255 (= A280), G256 (= G281), D257 (= D282), M259 (≠ F284), I281 (≠ N306), S284 (≠ G309), I288 (≠ V313)
binding 3-deoxy-alpha-D-erythro-hex-2-ulofuranosonic acid: L10 (≠ S17), A32 (≠ G40), G33 (= G41), S34 (= S42), N37 (= N45), Y89 (≠ V97), L103 (= L109), Y105 (≠ F111), R107 (= R113), I136 (≠ T142), R165 (= R174), T253 (≠ L278), G254 (= G279), D257 (= D282), D293 (≠ C318)
binding 2-keto-3-deoxygluconate: L10 (≠ S17), G33 (= G41), S34 (= S42), N37 (= N45), Y89 (≠ V97), L103 (= L109), Y105 (≠ F111), R107 (= R113), I136 (≠ T142), R165 (= R174), T253 (≠ L278), G254 (= G279), D257 (= D282), D293 (≠ C318)

Q97U29 2-dehydro-3-deoxygluconokinase/2-dehydro-3-deoxygalactonokinase; 2-dehydro-3-deoxyglucono/galactono-kinase; 2-keto-3-deoxy-galactonokinase; 2-keto-3-deoxygluconokinase; 3-deoxy-2-oxo-D-gluconate kinase; KDG kinase; KDGal kinase; EC 2.7.1.178 from Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) (Sulfolobus solfataricus) (see paper)
25% identity, 51% coverage: 8:334/642 of query aligns to 2:310/313 of Q97U29

query
sites
Q97U29

L

V

D

D

L

V

I

I

A

A

V

L

G

G

R

E

S

P

S

L

V

I

D

Q

L

-

Y

F

G

N

Q

S

Q

F

V

N

G

P

G

G

R

P

L

L

E

R

D

F

M

V

G

N

S

Y

F

F

A

E

K

K

Y

H

L

V

G

A

G
|
G

S
|
S

P
x
E

T
x
L

N
|
N

T

F

A

C

A

I

G

A

G

V

A

V

R

R

L

N

G

H

L

L

K

S

T

C

G

S

L

L

L

I

T

A

R

R

V

V

G

G

A

N

D

D

H

E

M

F

G

G

R

K

F

N

I

I

R

I

E

E

Q

Y

L

S

E

R

R

A

E

Q

G

G

V

I

D

D

V

T

A

S

G

H

V

I

L

K

S

V

D

D

P

N

D

E

R

S

L

F

T

T

A

G

L

I

V
x
Y

I

F

L

I

-

Q

-

R

G

G

I

Y

R

P

D

I

R

P

V

M

N

K

F

S

P

E

L

L

I

V

F
x
Y

Y
|
Y

R
|
R

E

K

N

G

C

S

A

A

D

G

M

S

A

R

L

L

E

S

P

P

S

E

D

D

V

I

D

N

E

E

A

N

W

Y

F

V

A

R

Q

N

A

S

G

R

A

L

V

V

L

H

I

S

N

T

G

G

T

I

H

T

L

L

S

A

-

I

Q

S

P

D

N

N

V

A

Y

K

E

E

T

A

S

V

L

I

K

K

A

A

A

F

R

E

A

L

V

A

K

K

A

S

A

R

G

-

R

-

V

-

A

S

F

L

D

D

I

T

D
x
N

Y
x
I

R
|
R

P

P

V

K

L

L

W

W

G

S

L

S

T

L

G

E

K

K

D

-

A

-

G

-

E

-

N

-

R

-

F

-

V

-

E

A

N

K

Q

E

Q

T

V

I

T

L

A

S

K

I

L

L

Q

K

E

K

V

Y

V

D

A

-

L

-

C

I

D

E

L

V

I

L

V

I

G

T

T

D

E

P

E

D

I

T

H

K

I

I

L

L

G

L

G

D

S

V

T

T

D

D

T

P

I

D

A

E

A

A

L

Y

R

R

A

K

I

Y

R

K

R

E

A

L

S

G

D

V

A

K

L

V

L

L

V

L

C

Y

K
|
K

R
x
L

G
|
G

P
x
S

E
x
K

G
|
G

C

A

V

I

A

A

F

Y

P

K

G

D

A

N

I

V

P

K

D

A

A

F

L

K

D

D

E

-

G

-

V

-

S

-

A

-

R

-

G

A

F

Y

K

K

V

V

E

P

V

V

F

E

N

D

V

P

L

T

G
|
G

A
|
A

G
|
G

D
|
D

A

A

F

M

M

A

A

G

G

T

F

F

L

V

R

S

G

L

W

Y

L

L

R

Q

H

G

E

K

S

D

V

I

E

E

T

Y

C

S

C

L

E

A

W

H

G

G

N

I

A

A

C

A

G

S

A

T

I

L

V

V

V

I

S

T

R

V

H

R

G

G

C
x
D

T

N

P

E

A

L

M

T

P

P

T

T

W

L

I

E

E

D

L

A

Q

E

A

R

F

F

L

L

S

N

E

E

GSELN 34:38 (≠ GSPTN 41:45) binding
Y90 (≠ V97) binding
YYR 106:108 (≠ FYR 111:113) binding
NIR 164:166 (≠ DYR 172:174) binding
R166 (= R174) binding
KLGSKG 226:231 (≠ KRGPEG 244:249) binding
GAGD 255:258 (= GAGD 279:282) binding
D258 (= D282) binding
D294 (≠ C318) binding

1tz3A Crystal structure of aminoimidazole riboside kinase complexed with aminoimidazole riboside (see paper)
27% identity, 50% coverage: 13:335/642 of query aligns to 6:299/299 of 1tz3A

query
sites
1tz3A

V

I

G

G

R
x
D

S

A

S
|
S

V

V

D
|
D

L

L

Y

V

G

P

Q

-

V

-

G

-

R

-

L

-

E

E

D

K

M

Q

G

N

S

S

F

Y

A

L

K

K

Y
x
C

L

P

G
|
G

S
x
A

P

S

T

A

N

N

T

V

A

G

A

V

G

C

G

V

A

A

R

R

L

L

G

G

L

G

K

E

T

C

G

G

L

F

L

I

T

G

R

C

V

L

G

G

A

D

D

D

H

D

M

A

G

G

R

R

F

F

I

L

R

R

E

Q

Q

V

L

F

E

Q

R

D

E

N

G

G

V

V

D

D

V

V

A

T

G

F

V

L

L

R

S

L

D

D

P

A

D

D

R

L

L

T

T

S

A

A

L

V

V
x
L

I

I

L

V

G

N

I

-

R

-

D

-

R

-

V

-

N

-

F

-

P

S

L
x
F

I

T

F
x
Y

Y

L

R

V

E

H

N

P

C

G

A

A

D

D

M

T

A

Y

L

V

E

S

P

P

S

Q

D

D

V

L

D

P

E

P

A

-

W

-

F

F

A

R

Q

Q

A

Y

G

E

A

W

V

F

L

Y

I

F

N

S

G

S

T

I

H

G

L

L

S

T

Q

D

P

R

N

P

V

A

Y

R

E

E

T

A

S

C

L

L

K

E

A

G

A

A

R

R

A

R

V

M

K

R

A

E

A

A

G

G

R

Y

V

V

A

L

F

F

D

D

I

V

D

N

Y

L

R
|
R

P

S

V

K

L
x
M

W

W

G

G

L

N

T

T

G

D

K

-

D

-

A

-

G

-

E

-

N

-

R

-

F

-

V

-

E

E

N

I

Q

P

Q

E

V

L

T

I

A

A

K

R

L

S

Q

A

E

A

V

L

V

A

A

S

L

I

C

C

D

K

L

V

I

-

V

-

G

-

T

S

E

A

E

D

E

E

I

L

H

C

I

Q

L

L

G

S

G

G

S

A

T

S

D

H

T

W

I

Q

A

D

A

A

L

R

R

Y

A

Y

I

L

R

R

R

D

-

L

-

G

-

C

-

D

-

T

-

I

-

S

-

L

-

G

A

A

S

D

D

G

A

A

L

L

V

I

C

T

K

A

R

-

G

-

P

-

E

E

G

G

C

E

V

F

A

H

F

F

P

P

G

A

A

P

I

-

P

-

D

-

A

-

L

-

D

-

E

-

G

-

V

-

S

-

A

-

R

-

G

-

F

-

K

R

V

V

E

D

V

V

F

V

N

D

V

T

L

T

G
|
G

A
|
A

G
|
G

D
|
D

A

A

F

F

M

V

A

G

G

G

F

L

L

L

R

F

G

T

W

L

L

S

R

R

-

A

-

N

-

C

-

W

-

D

H

H

E

A

S

L

V

L

E

A

T

E

C

A

C

I

E

S

W

N

G

A

N

N

A

A

C

C

G

G

A

A

I

M

V

A

V

V

S

T

R

A

H

K

G

G

C

A

T

M

P

T

A

A

M

L

P

P

T

F

W

P

I

D

E

Q

L

L

Q

N

A

T

F

F

L

L

S

S

E

S

R

H

active site: C24 (≠ Y38), F88 (≠ L109), G238 (= G279), A239 (= A280), G240 (= G281), D241 (= D282)
binding 5-aminoimidazole ribonucleoside: D8 (≠ R15), S10 (= S17), D12 (= D19), G26 (= G40), G27 (= G41), A28 (≠ S42), L83 (≠ V97), F88 (≠ L109), Y90 (≠ F111), R151 (= R174), M154 (≠ L177), D241 (= D282)

1tz6A Crystal structure of aminoimidazole riboside kinase from salmonella enterica complexed with aminoimidazole riboside and atp analog (see paper)
27% identity, 50% coverage: 13:333/642 of query aligns to 6:297/297 of 1tz6A

query
sites
1tz6A

V

I

G

G

R
x
D

S

A

S
|
S

V

V

D
|
D

L

L

Y

V

G

P

Q

-

V

-

G

-

R

-

L

-

E

E

D

K

M

Q

G

N

S

S

F

Y

A

L

K

K

Y
x
C

L

P

G
|
G

S
x
A

P

S

T

A

N
|
N

T

V

A

G

A

V

G

C

G

V

A

A

R

R

L

L

G

G

L

G

K

E

T

C

G

G

L

F

L

I

T

G

R

C

V

L

G

G

A

D

D

D

H

D

M

A

G

G

R

R

F

F

I

L

R

R

E

Q

Q

V

L

F

E

Q

R

D

E

N

G

G

V

V

D

D

V

V

A

T

G

F

V

L

L

R

S

L

D

D

P

A

D

D

R

L

L

T

T

S

A

A

L

V

V
x
L

I

I

L

V

G

N

I

-

R

-

D

-

R

-

V

-

N

-

F

-

P

S

L
x
F

I

T

F
x
Y

Y

L

R

V

E

H

N

P

C

G

A

A

D

D

M

T

A

Y

L

V

E

S

P

P

S

Q

D

D

V

L

D

P

E

P

A

-

W

-

F

F

A

R

Q

Q

A

Y

G

E

A

W

V

F

L

Y

I

F

N

S

G

S

T

I

H

G

L

L

S

T

Q

D

P

R

N

P

V

A

Y

R

E

E

T

A

S

C

L

L

K

E

A

G

A

A

R

R

A

R

V

M

K

R

A

E

A

A

G

G

R

Y

V

V

A

L

F

F

D
|
D

I

V

D
x
N

Y

L

R
|
R

P

S

V

K

L
x
M

W

W

G

G

L

N

T

T

G

D

K

-

D

-

A

-

G

-

E

-

N

-

R

-

F

-

V

-

E

E

N

I

Q

P

Q

E

V

L

T

I

A

A

K

R

L

S

Q

A

E

A

V

L

V

A

A

S

L

I

C

C

D
x
K

L

V

I

-

V

-

G

-

T
x
S

E

A

E

D

E
|
E

I

L

H

C

I

Q

L

L

G

S

G

G

S

A

T

S

D

H

T

W

I

Q

A

D

A

A

L

R

R

Y

A

Y

I

L

R

R

R

D

-

L

-

G

-

C

-

D

-

T

-

I

-
x
S

-

L

-
x
G

A
|
A

S

D

D
x
G

A

A

L

L

V

I

C

T

K

A

R

-

G

-

P

-

E

E

G

G

C

E

V

F

A

H

F

F

P

P

G
x
A

A

P

I

-

P

-

D

-

A

-

L

-

D

-

E

-

G

-

V

-

S

-

A

-

R

-

G

-

F

-

K

R

V
|
V

E

D

V
|
V

F

V

N

D

V

T

L
x
T

G
|
G

A
|
A

G
|
G

D
|
D

A

A

F
|
F

M

V

A

G

G

G

F

L

L

L

R

F

G

T

W

L

L

S

R

R

-

A

-

N

-

C

-

W

-

D

H

H

E

A

S

L

V

L

E

A

T

E

C

A

C

I

E

S

W

N

G

A

N
|
N

A

A

C

C

G
|
G

A
|
A

I

M

V

A

V
|
V

S

T

R

A

H

K

G

G

C

A

T

M

P

T

A

A

M

L

P

P

T

F

W

P

I

D

E

Q

L

L

Q

N

A

T

F

F

L

L

S

S

active site: C24 (≠ Y38), F88 (≠ L109), G238 (=