SitesBLAST
Comparing CCNA_01414 FitnessBrowser__Caulo:CCNA_01414 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
3bptA Crystal structure of human beta-hydroxyisobutyryl-coa hydrolase in complex with quercetin
43% identity, 96% coverage: 6:340/350 of query aligns to 6:348/362 of 3bptA
- active site: G67 (= G67), P84 (≠ R84), R88 (≠ H88), G115 (= G115), G118 (= G118), E138 (= E138), D146 (= D146)
- binding (2r)-3-hydroxy-2-methylpropanoic acid: G66 (= G66), G67 (= G67), I69 (= I69), E90 (= E90), G114 (= G114), G115 (= G115), E138 (= E138), D146 (= D146), V147 (= V147)
- binding 3,5,7,3',4'-pentahydroxyflavone: F25 (≠ A25), L26 (= L26), A28 (= A28), G66 (= G66), G67 (= G67), I69 (= I69), P137 (= P137), I141 (= I141), L319 (≠ I311)
4hdtA Crystal structure of a carnitinyl-coa dehydratase from mycobacterium thermoresistibile (see paper)
40% identity, 96% coverage: 6:342/350 of query aligns to 3:333/340 of 4hdtA
- active site: G64 (= G67), I69 (≠ L72), W84 (≠ F87), Y88 (= Y91), G112 (= G115), G115 (= G118), E135 (= E138), P142 (= P145), D143 (= D146), R283 (≠ A288)
- binding zinc ion: H28 (≠ L31), E42 (≠ Q45), E57 (= E60), E79 (= E82), H93 (≠ L96), H185 (≠ F188)
Sites not aligning to the query:
Q9LKJ1 3-hydroxyisobutyryl-CoA hydrolase 1; CoA-thioester hydrolase CHY1; EC 3.1.2.-; EC 3.1.2.4 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
36% identity, 96% coverage: 1:337/350 of query aligns to 5:353/378 of Q9LKJ1
- G70 (= G67) mutation to S: Loss of activity.
- E142 (= E138) mutation to A: Loss of activity.
- D150 (= D146) mutation to G: Reduced activity.
5zaiC Crystal structure of 3-hydroxypropionyl-coa dehydratase from metallosphaera sedula (see paper)
32% identity, 52% coverage: 10:192/350 of query aligns to 9:188/259 of 5zaiC
- active site: A65 (≠ G67), F70 (≠ L72), S82 (≠ F87), R86 (≠ Y91), G110 (= G115), E113 (≠ G118), P132 (= P137), E133 (= E138), I138 (≠ L143), P140 (= P145), G141 (≠ D146)
- binding coenzyme a: K24 (≠ A25), L25 (= L26), A63 (= A65), G64 (= G66), A65 (≠ G67), D66 (= D68), I67 (= I69), P132 (= P137), R166 (= R170)
Sites not aligning to the query:
2hw5C The crystal structure of human enoyl-coenzyme a (coa) hydratase short chain 1, echs1
30% identity, 67% coverage: 15:250/350 of query aligns to 17:241/260 of 2hw5C
- active site: A68 (≠ G67), M73 (≠ L72), S83 (≠ E82), L87 (≠ F86), G111 (= G115), E114 (≠ G118), P133 (= P137), E134 (= E138), T139 (≠ L143), P141 (= P145), G142 (≠ D146), K227 (≠ S236), F237 (≠ L246)
- binding crotonyl coenzyme a: K26 (= K24), A27 (= A25), L28 (= L26), A30 (= A28), K62 (≠ R61), I70 (= I69), F109 (≠ M113)
1ey3A Structure of enoyl-coa hydratase complexed with the substrate dac-coa (see paper)
30% identity, 68% coverage: 14:250/350 of query aligns to 14:239/258 of 1ey3A
- active site: A66 (≠ G67), M71 (≠ L72), S81 (≠ E82), L85 (≠ F86), G109 (= G115), E112 (≠ G118), P131 (= P137), E132 (= E138), T137 (≠ L143), P139 (= P145), G140 (≠ D146), K225 (≠ S236), F235 (≠ L246)
- binding 4-(n,n-dimethylamino)cinnamoyl-coa: K24 (= K24), L26 (= L26), A28 (= A28), A64 (= A65), G65 (= G66), A66 (≠ G67), D67 (= D68), I68 (= I69), L85 (≠ F86), W88 (≠ T89), G109 (= G115), P131 (= P137), L135 (≠ I141), G140 (≠ D146)
1dubA 2-enoyl-coa hydratase, data collected at 100 k, ph 6.5 (see paper)
30% identity, 68% coverage: 14:250/350 of query aligns to 16:241/260 of 1dubA
- active site: A68 (≠ G67), M73 (≠ L72), S83 (≠ E82), L87 (≠ F86), G111 (= G115), E114 (≠ G118), P133 (= P137), E134 (= E138), T139 (≠ L143), P141 (= P145), G142 (≠ D146), K227 (≠ S236), F237 (≠ L246)
- binding acetoacetyl-coenzyme a: K26 (= K24), A27 (= A25), L28 (= L26), A30 (= A28), A66 (= A65), A68 (≠ G67), D69 (= D68), I70 (= I69), Y107 (≠ I111), G110 (= G114), G111 (= G115), E114 (≠ G118), P133 (= P137), E134 (= E138), L137 (≠ I141), G142 (≠ D146), F233 (≠ E242)
Sites not aligning to the query:
P14604 Enoyl-CoA hydratase, mitochondrial; mECH; mECH1; Enoyl-CoA hydratase 1; ECHS1; Short-chain enoyl-CoA hydratase; SCEH; EC 4.2.1.17; EC 5.3.3.8 from Rattus norvegicus (Rat) (see 3 papers)
30% identity, 68% coverage: 14:250/350 of query aligns to 46:271/290 of P14604
- E144 (≠ G118) mutation to D: Reduces activity 50-fold.; mutation to Q: Reduces activity 3300-fold.
- E164 (= E138) mutation to D: Reduces activity 1250-fold.; mutation to Q: Reduces activity 330000-fold.
Sites not aligning to the query:
- 1:29 modified: transit peptide, Mitochondrion
3q0jC Crystal structure of the mycobacterium tuberculosis crotonase in complex with the inhibitor acetoacetylcoa
30% identity, 65% coverage: 7:232/350 of query aligns to 6:215/255 of 3q0jC
- active site: A65 (≠ G67), M70 (≠ L72), T80 (≠ E82), F84 (= F87), G108 (= G115), E111 (≠ G118), P130 (= P137), E131 (= E138), V136 (≠ L143), P138 (= P145), G139 (≠ D146)
- binding acetoacetyl-coenzyme a: Q23 (≠ K24), A24 (= A25), L25 (= L26), A27 (= A28), A63 (= A65), G64 (= G66), A65 (≠ G67), D66 (= D68), I67 (= I69), K68 (≠ R70), M70 (≠ L72), F84 (= F87), G107 (= G114), G108 (= G115), E111 (≠ G118), P130 (= P137), E131 (= E138), P138 (= P145), G139 (≠ D146), M140 (≠ V147)
Sites not aligning to the query:
3q0gC Crystal structure of the mycobacterium tuberculosis crotonase bound to a reaction intermediate derived from crotonyl coa
30% identity, 65% coverage: 7:232/350 of query aligns to 6:215/255 of 3q0gC
- active site: A65 (≠ G67), M70 (≠ L72), T80 (≠ E82), F84 (= F87), G108 (= G115), E111 (≠ G118), P130 (= P137), E131 (= E138), V136 (≠ L143), P138 (= P145), G139 (≠ D146)
- binding coenzyme a: L25 (= L26), A63 (= A65), I67 (= I69), K68 (≠ R70), Y104 (≠ I111), P130 (= P137), E131 (= E138), L134 (≠ I141)
Sites not aligning to the query:
3h81A Crystal structure of enoyl-coa hydratase from mycobacterium tuberculosis (see paper)
30% identity, 65% coverage: 7:232/350 of query aligns to 5:214/256 of 3h81A
- active site: A64 (≠ G67), M69 (≠ L72), T79 (≠ E82), F83 (= F87), G107 (= G115), E110 (≠ G118), P129 (= P137), E130 (= E138), V135 (≠ L143), P137 (= P145), G138 (≠ D146)
Sites not aligning to the query:
2dubA Enoyl-coa hydratase complexed with octanoyl-coa (see paper)
30% identity, 68% coverage: 14:250/350 of query aligns to 15:235/254 of 2dubA
- active site: A67 (≠ G67), M72 (≠ L72), S82 (≠ Q92), G105 (= G115), E108 (≠ G118), P127 (= P137), E128 (= E138), T133 (≠ L143), P135 (= P145), G136 (≠ D146), K221 (≠ S236), F231 (≠ L246)
- binding octanoyl-coenzyme a: K25 (= K24), A26 (= A25), L27 (= L26), A29 (= A28), A65 (= A65), A67 (≠ G67), D68 (= D68), I69 (= I69), K70 (≠ R70), G105 (= G115), E108 (≠ G118), P127 (= P137), E128 (= E138), G136 (≠ D146), A137 (≠ V147)
3q0gD Crystal structure of the mycobacterium tuberculosis crotonase bound to a reaction intermediate derived from crotonyl coa
30% identity, 65% coverage: 7:232/350 of query aligns to 5:210/250 of 3q0gD
- active site: A64 (≠ G67), M69 (= M71), T75 (≠ A77), F79 (= F87), G103 (= G115), E106 (≠ G118), P125 (= P137), E126 (= E138), V131 (≠ L143), P133 (= P145), G134 (≠ D146)
Sites not aligning to the query:
1mj3A Crystal structure analysis of rat enoyl-coa hydratase in complex with hexadienoyl-coa (see paper)
31% identity, 68% coverage: 14:250/350 of query aligns to 16:239/258 of 1mj3A
- active site: A68 (≠ G67), M73 (≠ L72), S83 (≠ Q92), L85 (= L93), G109 (= G115), E112 (≠ G118), P131 (= P137), E132 (= E138), T137 (≠ L143), P139 (= P145), G140 (≠ D146), K225 (≠ S236), F235 (≠ L246)
- binding hexanoyl-coenzyme a: K26 (= K24), A27 (= A25), L28 (= L26), A30 (= A28), A66 (= A65), G67 (= G66), A68 (≠ G67), D69 (= D68), I70 (= I69), G109 (= G115), P131 (= P137), E132 (= E138), L135 (≠ I141), G140 (≠ D146)
3p85A Crystal structure enoyl-coa hydratase from mycobacterium avium (see paper)
33% identity, 55% coverage: 7:200/350 of query aligns to 3:178/224 of 3p85A
- active site: L62 (≠ G67), L67 (= L72), P68 (≠ A73), G92 (= G115), E95 (≠ G118), T114 (≠ P137), H115 (≠ E138), L120 (= L143), P122 (= P145), T123 (≠ D146)
- binding calcium ion: D43 (= D47), D45 (≠ E49)
Sites not aligning to the query:
6slbAAA Enoyl-CoA hydratase/carnithine racemase (see paper)
31% identity, 48% coverage: 18:186/350 of query aligns to 16:180/257 of 6slbAAA
- active site: Q64 (≠ G67), F69 (≠ L72), L80 (≠ A83), N84 (≠ F87), A108 (≠ G115), S111 (≠ G118), A130 (≠ P137), F131 (≠ E138), L136 (= L143), P138 (= P145), D139 (= D146)
- binding (~{E})-6-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethylsulfanyl]-6-oxidanylidene-hex-3-enoic acid: R58 (= R61), A62 (= A65), Q64 (≠ G67), D65 (= D68), L66 (≠ I69), Y76 (≠ D79), A108 (≠ G115), F131 (≠ E138), D139 (= D146)
Sites not aligning to the query:
Q7CQ56 1,4-dihydroxy-2-naphthoyl-CoA synthase; DHNA-CoA synthase; EC 4.1.3.36 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
31% identity, 43% coverage: 9:159/350 of query aligns to 25:177/285 of Q7CQ56
6z1pBI mS93 (see paper)
28% identity, 47% coverage: 20:182/350 of query aligns to 37:201/1413 of 6z1pBI
- active site: T85 (≠ G67), S134 (≠ G115), E157 (= E138), D165 (= D146)
- binding : Y41 (≠ K24), K42 (≠ A25), Q43 (≠ L26), T45 (≠ A28), D47 (≠ T30), H49 (≠ G32), K83 (≠ A65), T85 (≠ G67), D86 (= D68), F87 (≠ I69), K88 (≠ R70), K92 (≠ D74), L130 (≠ I111), K152 (≠ T133)
Sites not aligning to the query:
6slaAAA Enoyl-CoA hydratase/carnithine racemase (see paper)
29% identity, 48% coverage: 18:186/350 of query aligns to 13:168/245 of 6slaAAA
- active site: Q61 (≠ G67), L68 (≠ A83), N72 (≠ F87), A96 (≠ G115), S99 (≠ G118), A118 (≠ P137), F119 (≠ E138), L124 (= L143), P126 (= P145), N127 (≠ D146)
- binding ~{S}-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethyl] 2-(2,5-dihydrooxepin-7-yl)ethanethioate: L21 (= L26), A59 (= A65), Q61 (≠ G67), D62 (= D68), L63 (≠ I69), L68 (≠ A83), Y71 (≠ F86), A94 (≠ M113), G95 (= G114), A96 (≠ G115), F119 (≠ E138), I122 (= I141), L124 (= L143), N127 (≠ D146)
Sites not aligning to the query:
3t88A Crystal structure of escherichia coli menb in complex with substrate analogue, osb-ncoa (see paper)
30% identity, 43% coverage: 9:159/350 of query aligns to 21:173/281 of 3t88A
- active site: G82 (= G67), R87 (≠ L72), Y93 (vs. gap), H101 (≠ R85), L105 (≠ T89), G129 (= G115), V132 (≠ G118), G152 (≠ E138), S157 (≠ L143), D159 (≠ P145), G160 (≠ D146)
- binding o-succinylbenzoyl-N-coenzyme A: Q39 (≠ K24), V40 (≠ A25), R41 (≠ L26), A43 (= A28), S80 (≠ A65), G81 (= G66), G82 (= G67), D83 (= D68), Q84 (≠ I69), K85 (≠ R70), Y93 (vs. gap), V104 (≠ H88), L105 (≠ T89), Y125 (≠ I111), G129 (= G115), T151 (≠ P137), V155 (≠ I141), F158 (= F144), D159 (≠ P145)
Sites not aligning to the query:
Query Sequence
>CCNA_01414 FitnessBrowser__Caulo:CCNA_01414
MTDDPEVLIRVEKNVGRITLNRPKALHALTLGMCETMIGALLDWQDDPEVYMVLIDHTGE
RGFCAGGDIRMLADSGAKDGVEARRFFHTEYQLNHLLFTYETPVVAVMDGIVMGGGVGIS
MPAHIRIATERTTFAMPETGIGLFPDVGGGWYLPRLPGKAGLWLALTGARIKGADCMRLG
IATHFVEFGAVEGLKKAIIADPRRIEETLRKYRADAGKASLLGFEQDLNRLFVGESVEEI
FEFLTLDSSDWGKAQLEVMKTKSPQTLKVAFEQLKRGAAMEDFADNMAMEYRIGSRVVMK
HDFIEGVRAVIVDKDNAPRWSPARVEDVTDAALAEIFAPLPPGEEWTPLT
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory