SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing CCNA_01417 FitnessBrowser__Caulo:CCNA_01417 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P18079 5-aminolevulinate synthase; 5-aminolevulinic acid synthase; Delta-ALA synthase; Delta-aminolevulinate synthase; EC 2.3.1.37 from Rhodobacter capsulatus (strain ATCC BAA-309 / NBRC 16581 / SB1003) (see paper)
59% identity, 99% coverage: 1:402/408 of query aligns to 1:404/409 of P18079

query
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P18079

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R21 (= R21) binding
S137 (= S137) binding
K156 (≠ R156) binding
S189 (= S189) binding in other chain
H217 (= H217) binding in other chain
T245 (= T245) binding in other chain
K248 (= K248) active site
S277 (≠ T277) binding
T278 (= T278) binding
T365 (= T363) binding

2bwpA 5-aminolevulinate synthase from rhodobacter capsulatus in complex with glycine (see paper)
59% identity, 97% coverage: 1:396/408 of query aligns to 1:398/398 of 2bwpA

query
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2bwpA

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active site: N54 (= N54), H142 (= H142), E185 (= E185), S189 (= S189), D214 (= D214), H217 (= H217), K248 (= K248)
binding n-glycine-[3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-yl-methane]: N54 (= N54), A115 (≠ G115), Y116 (= Y116), H142 (= H142), E185 (= E185), D214 (= D214), V216 (= V216), H217 (= H217), T245 (= T245), K248 (= K248), S277 (≠ T277), T278 (= T278)

2bwoB 5-aminolevulinate synthase from rhodobacter capsulatus in complex with succinyl-coa (see paper)
59% identity, 97% coverage: 1:396/408 of query aligns to 1:398/399 of 2bwoB

query
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2bwoB

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active site: N54 (= N54), H142 (= H142), E185 (= E185), S189 (= S189), D214 (= D214), H217 (= H217), K248 (= K248)
binding pyridoxal-5'-phosphate: S114 (= S114), A115 (≠ G115), Y116 (= Y116), H142 (= H142), E185 (= E185), S189 (= S189), D214 (= D214), V216 (= V216), H217 (= H217), T245 (= T245), K248 (= K248)
binding succinyl-coenzyme a: R21 (= R21), T83 (= T83), N85 (= N85), S137 (= S137), S139 (≠ E139), H142 (= H142), I146 (= I146), K150 (≠ R150), K156 (≠ R156), I158 (= I158), F276 (= F276), F363 (≠ Y361), P364 (= P362), T365 (= T363)

P22557 5-aminolevulinate synthase, erythroid-specific, mitochondrial; ALAS-E; 5-aminolevulinic acid synthase 2; Delta-ALA synthase 2; Delta-aminolevulinate synthase 2; EC 2.3.1.37 from Homo sapiens (Human) (see 11 papers)
47% identity, 99% coverage: 1:402/408 of query aligns to 143:559/587 of P22557

query
sites
P22557

M

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R

K156 (≠ I14) to E: in SIDBA1; significantly reduced activity
R170 (= R28) to H: in SIDBA1; significantly increased thermosensitivity
R204 (≠ Q61) to Q: in SIDBA1; 15% to 35% activity of wild-type; dbSNP:rs1338391423
R218 (≠ E75) to H: in SIDBA1; significantly increased thermosensitivity; dbSNP:rs185504937
E242 (= E99) to K: in SIDBA1; significantly reduced activity
C258 (≠ G115) binding in other chain
F259 (≠ Y116) binding in other chain
D263 (≠ E120) to N: in SIDBA1; significantly reduced activity
P339 (≠ D196) to L: in SIDBA1; significantly reduced activity
H360 (= H217) binding in other chain
R375 (= R232) to C: in SIDBA1; significantly reduced activity
T388 (= T245) binding in other chain
K391 (= K248) modified: N6-(pyridoxal phosphate)lysine
R411 (= R268) to C: in SIDBA1; 12% to 25% activity of wild-type; dbSNP:rs137852305; to H: in SIDBA1; significantly reduced activity
T420 (= T277) binding
T421 (= T278) binding
R452 (≠ E307) to G: in SIDBA1; does not affect activity
R511 (= R366) mutation to E: 2-fold increased enzyme activity with a lower specificity for glycine and higher for succinyl-CoA.
P520 (= P375) to L: in SIDBA1; likely benign; associated in cis with H-560 in a patient; dbSNP:rs201062903

Sites not aligning to the query:

1:49 modified: transit peptide, Mitochondrion
560 R → H: in SIDBA1; associated in cis with L-520 in a patient; dbSNP:rs892041887
572 R → H: in SIDBA1; does not affect activity
586 Y → F: rare variant found in a congenital erythropoietic porphyria patient that also carries UROS mutations R-73 and Q-248; increased enzyme activity; dbSNP:rs139596860

5qrdB Pandda analysis group deposition -- crystal structure of human alas2a in complex with z1328968520
48% identity, 97% coverage: 1:396/408 of query aligns to 7:400/428 of 5qrdB

query
sites
5qrdB

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binding pyridoxal-5'-phosphate: S116 (= S114), C117 (≠ G115), F118 (≠ Y116), N121 (= N119), H144 (= H142), E187 (= E185), D216 (= D214), V218 (= V216), H219 (= H217), T247 (= T245), K250 (= K248), T279 (= T277), T280 (= T278)

Sites not aligning to the query:

binding 4-[(2-methylsulfonylimidazol-1-yl)methyl]-1,3-thiazole: 423, 424

5qrbB Pandda analysis group deposition -- crystal structure of human alas2a in complex with z2856434868
48% identity, 97% coverage: 1:396/408 of query aligns to 7:400/428 of 5qrbB

query
sites
5qrbB

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N

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L

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W

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T

binding 1-ethyl-N-(2-fluorophenyl)piperidin-4-amine: W35 (≠ Q29), A38 (≠ Q32), F41 (≠ R35), D49 (≠ E47)
binding pyridoxal-5'-phosphate: S116 (= S114), C117 (≠ G115), F118 (≠ Y116), H144 (= H142), E187 (= E185), S191 (= S189), D216 (= D214), V218 (= V216), H219 (= H217), T247 (= T245), K250 (= K248), T279 (= T277), T280 (= T278)

5qr8B Pandda analysis group deposition -- crystal structure of human alas2a in complex with z57258487
48% identity, 97% coverage: 1:396/408 of query aligns to 7:400/428 of 5qr8B

query
sites
5qr8B

M
x
F

D
x
S

Y

Y

K
x
D

A

Q

A

F

F

F

R

R

S

D

A

K

V

I

E

M

Q

E

I

K

R

K

E

Q

E

D

G

H

R

T

Y

Y

R

R

V

V

F

F

A

K

D

T

L

V

K

N

R

R

Q

W

R

A

G

D

Q

A

F

Y

P

P

R

F

A

A

T

-

W

-

T

-

R

-

Q

Q

D

H

G

F

S

S

E

S

H

K

E

D

V

V

V

S

V

V

W

W

C

C

S

S

N

N

D

D

Y

Y

L

L

G

G

Q

M

G

S

Q

R

N

H

P

P

V

Q

V

V

L

L

E

Q

A

A

M

T

K

Q

A

E

A

T

V

L

D

Q

E

R

H

H

G

G

S

V

G

G

S

A

G

G

T

T

R

R

N

N

I

I

S

S

G

G

T

T

N

S

H

K

D

F

H

H

V

V

L

E

L

L

E

E

Q

Q

E

E

L

L

A

A

D

E

L

L

H

H

G

Q

K

K

E

D

A

S

G

A

L

L

F

F

T

S

S

S

G
x
C

Y
x
F

V

V

S

A

N

N

E

D

A

S

T

T

L

L

S

F

V

T

V

L

Q

A

K

K

I

I

L

L

P

P

G

G

L

C

I

E

I

I

F

Y

S

S

D

D

E

A

L

G

N

N

H
|
H

A

A

S

S

M

M

I

I

A

Q

G

G

I

I

R

R

N

N

G

S

G

G

G

A

P

A

R

K

K

F

I

V

F

F

K

R

H

H

N

N

D

D

L

P

A

D

H

H

L

L

E

K

Q

K

L

L

A

E

E

K

A

S

P

N

A

P

D

K

A

I

P

P

K

K

L

I

I

V

A

A

F

F

E
|
E

S

T

V

V

Y

H

S
|
S

M

M

D

D

G

G

D

A

I

I

A

C

D

P

L

L

A

E

G

E

T

L

V

C

A

D

L

V

A

S

K

H

K

Q

Y

Y

G

G

A

A

M

L

T

T

Y

F

L

V

D
|
D

E

E

V
|
V

H
|
H

A

A

V

V

G

G

M

L

Y

Y

G

G

P

S

R

R

G

G

G

A

G

G

V

I

A

G

E

E

R

R

D

D

G

G

L

I

M

M

G

H

E

K

I

I

D

D

I

I

E

S

G

G

T
|
T

L

L

G

G

K
|
K

A

A

F

F

G

G

V

C

M

V

G

G

Y

Y

I

I

T

A

G

S

D

T

A

R

E

D

V

L

I

V

D

D

A

M

I

V

R

R

L

S

M

Y

A

A

S

A

G

G

F

F

I

I

F

F

T
|
T

S

S

L

L

P

P

A

M

L

V

T

L

A

S

G

G

A

A

L

L

A

E

S

S

V

V

R

R

W

L

L

L

K

K

-

G

-

E

Q

E

H

G

P

Q

E

A

V

L

R

R

E

R

I

A

H

H

Q

Q

E

R

R

N

A

V

A

K

T

H

L

M

K

R

A

Q

M

L

F

L

K

M

A

D

A

R

G

G

L

L

P

P

V

V

M

I

D

P

S

C

V

P

S

S

H

H

I

I

V

I

P

P

V

I

L

R

V

V

G

G

D

N

P

A

V

A

H

L

C

N

K

S

M

K

I

L

S

C

D

D

M

L

L

L

A

S

D

K

F

H

G

G

V

I

Y

Y

V

V

Q

Q

P

A

I

I

N

N

Y

Y

P

P

T

T

V

V

P

P

R

R

G

G

T

E

E

E

R

L

L

L

R

R

F

L

T

A

P

P

T

S

P

P

F

H

H

H

T

S

D

P

D

Q

M

M

R

E

K

D

L

F

V

V

A

E

A

K

M

L

E

L

K

L

L

A

W

W

A

T

binding N-{[4-(dimethylamino)phenyl]methyl}-4H-1,2,4-triazol-4-amine: F7 (≠ M1), S8 (≠ D2), D10 (≠ K4)
binding pyridoxal-5'-phosphate: C117 (≠ G115), F118 (≠ Y116), H144 (= H142), E187 (= E185), S191 (= S189), D216 (= D214), V218 (= V216), H219 (= H217), T247 (= T245), K250 (= K248), T279 (= T277), T280 (= T278)

Sites not aligning to the query:

binding N-{[4-(dimethylamino)phenyl]methyl}-4H-1,2,4-triazol-4-amine: 6

5qr7B Pandda analysis group deposition -- crystal structure of human alas2a in complex with z57299529
48% identity, 97% coverage: 1:396/408 of query aligns to 7:400/428 of 5qr7B

query
sites
5qr7B

M

F

D

S

Y

Y

K

D

A

Q

A

F

F

F

R

R

S

D

A

K

V

I

E

M

Q

E

I

K

R

K

E

Q

E

D

G

H

R

T

Y

Y

R

R

V

V

F

F

A

K

D

T

L

V

K

N

R

R

Q
x
W

R

A

G

D

Q
x
A

F

Y

P

P

R
x
F

A

A

T

-

W

-

T

-

R

-

Q

Q

D

H

G

F

S

S

E

S

H

K

E
x
D

V

V

V

S

V

V

W

W

C

C

S

S

N

N

D

D

Y

Y

L

L

G

G

Q

M

G

S

Q

R

N

H

P

P

V

Q

V

V

L

L

E

Q

A

A

M

T

K

Q

A

E

A

T

V

L

D

Q

E

R

H

H

G

G

S

V

G

G

S

A

G

G

T

T

R

R

N

N

I

I

S

S

G

G

T

T

N

S

H

K

D

F

H

H

V

V

L

E

L

L

E

E

Q

Q

E

E

L

L

A

A

D

E

L

L

H

H

G

Q

K

K

E

D

A

S

G

A

L

L

F

F

T

S

S

S

G
x
C

Y
x
F

V

V

S

A

N

N

E

D

A

S

T

T

L

L

S

F

V

T

V

L

Q

A

K

K

I

I

L

L

P

P

G

G

L

C

I

E

I

I

F

Y

S

S

D

D

E

A

L

G

N

N

H
|
H

A

A

S

S

M

M

I

I

A

Q

G

G

I

I

R

R

N

N

G

S

G

G

G

A

P

A

R

K

K

F

I

V

F

F

K

R

H

H

N

N

D

D

L

P

A

D

H

H

L

L

E

K

Q

K

L

L

A

E

E

K

A

S

P

N

A

P

D

K

A

I

P

P

K

K

L

I

I

V

A

A

F

F

E
|
E

S

T

V

V

Y

H

S

S

M

M

D

D

G

G

D

A

I

I

A

C

D

P

L

L

A

E

G

E

T

L

V

C

A

D

L

V

A

S

K
x
H

K

Q

Y

Y

G

G

A

A

M
x
L

T

T

Y

F

L

V

D
|
D

E

E

V
|
V

H
|
H

A

A

V

V

G

G

M

L

Y

Y

G

G

P

S

R

R

G

G

G

A

G

G

V

I

A

G

E

E

R

R

D

D

G

G

L

I

M

M

G
x
H

E

K

I

I

D
|
D

I

I

E

S

G

G

T

T

L

L

G

G

K
|
K

A

A

F

F

G

G

V

C

M

V

G

G

Y

Y

I

I

T

A

G

S

D
x
T

A

R

E

D

V

L

I

V

D

D

A

M

I

V

R

R

L

S

M

Y

A

A

S

A

G

G

F

F

I

I

F

F

T
|
T

S

S

L

L

P

P

A

M

L

V

T

L

A

S

G

G

A

A

L

L

A

E

S

S

V

V

R

R

W

L

L

L

K

K

-

G

-

E

Q

E

H

G

P

Q

E

A

V

L

R

R

E

R

I

A

H

H

Q

Q

E

R

R

N

A

V

A

K

T

H

L

M

K

R

A

Q

M

L

F

L

K

M

A

D

A

R

G

G

L

L

P

P

V

V

M

I

D

P

S

C

V

P

S

S

H

H

I

I

V

I

P

P

V

I

L

R

V

V

G

G

D

N

P

A

V

A

H

L

C

N

K

S

M

K

I

L

S

C

D

D

M

L

L

L

A

S

D

K

F

H

G

G

V

I

Y

Y

V

V

Q

Q

P

A

I

I

N

N

Y

Y

P

P

T

T

V

V

P

P

R

R

G

G

T

E

E

E

R

L

L

L

R

R

F

L

T

A

P

P

T

S

P

P

F

H

H

H

T

S

D

P

D

Q

M

M

R

E

K

D

L

F

V

V

A

E

A

K

M

L

E

L

K

L

L

A

W

W

A

T

binding ~{N}-(2-phenylethyl)-1~{H}-benzimidazol-2-amine: W35 (≠ Q29), A38 (≠ Q32), F41 (≠ R35), D49 (≠ E47), H207 (≠ K205), L212 (≠ M210), H239 (≠ G237), D242 (= D240), T262 (≠ D260)
binding pyridoxal-5'-phosphate: C117 (≠ G115), F118 (≠ Y116), H144 (= H142), E187 (= E185), D216 (= D214), V218 (= V216), H219 (= H217), K250 (= K248), T279 (= T277), T280 (= T278)

5qr5B Pandda analysis group deposition -- crystal structure of human alas2a in complex with z2241115980
48% identity, 97% coverage: 1:396/408 of query aligns to 7:400/428 of 5qr5B

query
sites
5qr5B

M

F

D

S

Y

Y

K

D

A

Q

A

F

F

F

R

R

S

D

A

K

V

I

E

M

Q

E

I

K

R

K

E

Q

E

D

G

H

R

T

Y

Y

R

R

V

V

F

F

A

K

D

T

L

V

K

N

R

R

Q
x
W

R

A

G

D

Q

A

F

Y

P

P

R
x
F

A

A

T

-

W

-

T

-

R

-

Q

Q

D

H

G

F

S

S

E

S

H

K

E
x
D

V

V

V

S

V

V

W

W

C

C

S

S

N

N

D

D

Y

Y

L

L

G

G

Q

M

G

S

Q

R

N

H

P

P

V

Q

V

V

L

L

E

Q

A

A

M

T

K

Q

A

E

A

T

V

L

D

Q

E

R

H

H

G

G

S

V

G

G

S

A

G

G

T

T

R

R

N

N

I

I

S

S

G

G

T

T

N

S

H

K

D

F

H

H

V

V

L

E

L

L

E

E

Q

Q

E

E

L

L

A

A

D

E

L

L

H

H

G

Q

K

K

E

D

A

S

G

A

L

L

F

F

T

S

S
|
S

G
x
C

Y
x
F

V

V

S

A

N
|
N

E

D

A

S

T

T

L

L

S

F

V

T

V

L

Q

A

K

K

I

I

L

L

P

P

G

G

L

C

I

E

I

I

F

Y

S

S

D

D

E

A

L

G

N

N

H
|
H

A

A

S

S

M

M

I

I

A

Q

G

G

I

I

R

R

N

N

G

S

G

G

G

A

P

A

R

K

K

F

I

V

F

F

K

R

H

H

N

N

D

D

L

P

A

D

H

H

L

L

E

K

Q

K

L

L

A

E

E

K

A

S

P

N

A

P

D

K

A

I

P

P

K

K

L

I

I

V

A

A

F

F

E
|
E

S

T

V

V

Y

H

S
|
S

M

M

D

D

G

G

D

A

I

I

A

C

D

P

L

L

A

E

G

E

T

L

V

C

A

D

L

V

A

S

K

H

K

Q

Y

Y

G

G

A

A

M

L

T

T

Y

F

L

V

D
|
D

E

E

V
|
V

H
|
H

A

A

V

V

G

G

M

L

Y

Y

G

G

P

S

R

R

G

G

G

A

G

G

V

I

A

G

E

E

R

R

D

D

G

G

L

I

M

M

G

H

E

K

I

I

D

D

I

I

E

S

G

G

T

T

L

L

G

G

K
|
K

A

A

F

F

G

G

V

C

M

V

G

G

Y

Y

I

I

T

A

G

S

D

T

A

R

E

D

V

L

I

V

D

D

A

M

I

V

R

R

L

S

M

Y

A

A

S

A

G

G

F

F

I

I

F

F

T
|
T

S

S

L

L

P

P

A

M

L

V

T

L

A

S

G

G

A

A

L

L

A

E

S

S

V

V

R

R

W

L

L

L

K

K

-

G

-

E

Q

E

H

G

P

Q

E

A

V

L

R

R

E

R

I

A

H

H

Q

Q

E

R

R

N

A

V

A

K

T

H

L

M

K

R

A

Q

M

L

F

L

K

M

A

D

A

R

G

G

L

L

P

P

V

V

M

I

D

P

S

C

V

P

S

S

H

H

I

I

V

I

P

P

V

I

L

R

V

V

G

G

D

N

P

A

V

A

H

L

C

N

K

S

M

K

I

L

S

C

D

D

M

L

L

L

A

S

D

K

F

H

G

G

V

I

Y

Y

V

V

Q

Q

P

A

I

I

N

N

Y

Y

P

P

T

T

V

V

P

P

R

R

G

G

T

E

E

E

R

L

L

L

R

R

F

L

T

A

P

P

T

S

P

P

F

H

H

H

T

S

D

P

D

Q

M

M

R

E

K

D

L

F

V

V

A

E

A

K

M

L

E

L

K

L

L

A

W

W

A

T

binding 1-[(furan-2-yl)methyl]-4-(methylsulfonyl)piperazine: W35 (≠ Q29), F41 (≠ R35), D49 (≠ E47)
binding pyridoxal-5'-phosphate: S116 (= S114), C117 (≠ G115), F118 (≠ Y116), N121 (= N119), H144 (= H142), E187 (= E185), S191 (= S189), D216 (= D214), V218 (= V216), H219 (= H217), K250 (= K248), T279 (= T277), T280 (= T278)

5qr4B Pandda analysis group deposition -- crystal structure of human alas2a in complex with z2856434826
48% identity, 97% coverage: 1:396/408 of query aligns to 7:400/428 of 5qr4B

query
sites
5qr4B

M

F

D

S

Y

Y

K

D

A

Q

A

F

F

F

R

R

S

D

A

K

V

I

E

M

Q

E

I

K

R

K

E

Q

E

D

G

H

R

T

Y

Y

R

R

V

V

F

F

A

K

D

T

L

V

K

N

R

R

Q
x
W

R

A

G
x
D

Q
x
A

F

Y

P

P

R
x
F

A

A

T

-

W

-

T

-

R

-

Q

Q

D

H

G

F

S

S

E

S

H

K

E

D

V

V

V

S

V

V

W

W

C

C

S

S

N

N

D

D

Y

Y

L

L

G

G

Q

M

G

S

Q

R

N

H

P

P

V

Q

V

V

L

L

E

Q

A

A

M

T

K

Q

A

E

A

T

V

L

D

Q

E

R

H

H

G

G

S

V

G

G

S

A

G

G

T

T

R

R

N

N

I

I

S

S

G

G

T

T

N

S

H

K

D

F

H

H

V

V

L

E

L

L

E

E

Q

Q

E

E

L

L

A

A

D

E

L

L

H

H

G

Q

K

K

E

D

A

S

G

A

L

L

F

F

T

S

S

S

G
x
C

Y
x
F

V

V

S

A

N
|
N

E

D

A

S

T

T

L

L

S

F

V

T

V

L

Q

A

K

K

I

I

L

L

P

P

G

G

L

C

I

E

I

I

F

Y

S

S

D

D

E

A

L

G

N

N

H
|
H

A

A

S

S

M

M

I

I

A

Q

G

G

I

I

R

R

N

N

G

S

G

G

G

A

P

A

R

K

K

F

I

V

F

F

K

R

H

H

N

N

D

D

L

P

A

D

H

H

L

L

E

K

Q

K

L

L

A

E

E

K

A

S

P

N

A

P

D

K

A

I

P

P

K

K

L

I

I

V

A

A

F

F

E
|
E

S

T

V

V

Y

H

S
|
S

M

M

D

D

G

G

D

A

I

I

A

C

D

P

L

L

A

E

G

E

T

L

V

C

A

D

L

V

A

S

K

H

K

Q

Y

Y

G

G

A

A

M

L

T

T

Y

F

L

V

D
|
D

E

E

V
|
V

H
|
H

A

A

V

V

G

G

M

L

Y

Y

G

G

P

S

R

R

G

G

G

A

G

G

V

I

A

G

E

E

R

R

D

D

G

G

L

I

M

M

G

H

E

K

I

I

D

D

I

I

E

S

G

G

T
|
T

L

L

G

G

K
|
K

A

A

F

F

G

G

V

C

M

V

G

G

Y

Y

I

I

T

A

G

S

D

T

A

R

E

D

V

L

I

V

D

D

A

M

I

V

R

R

L

S

M

Y

A

A

S

A

G

G

F

F

I

I

F

F

T
|
T

S

S

L

L

P

P

A

M

L

V

T

L

A

S

G

G

A

A

L

L

A

E

S

S

V

V

R

R

W

L

L

L

K

K

-

G

-

E

Q

E

H

G

P

Q

E

A

V

L

R

R

E

R

I

A

H

H

Q

Q

E

R

R

N

A

V

A

K

T

H

L

M

K

R

A

Q

M

L

F

L

K

M

A

D

A

R

G

G

L

L

P

P

V

V

M

I

D

P

S

C

V

P

S

S

H

H

I

I

V

I

P

P

V

I

L

R

V

V

G

G

D

N

P

A

V

A

H

L

C

N

K

S

M

K

I

L

S

C

D

D

M

L

L

L

A

S

D

K

F

H

G

G

V

I

Y

Y

V

V

Q

Q

P

A

I

I

N

N

Y

Y

P

P

T

T

V

V

P

P

R

R

G

G

T

E

E

E

R

L

L

L

R

R

F

L

T

A

P

P

T

S

P

P

F

H

H

H

T

S

D

P

D

Q

M

M

R

E

K

D

L

F

V

V

A

E

A

K

M

L

E

L

K

L

L

A

W

W

A

T

binding [4-(propan-2-yl)piperazin-1-yl](thiophen-2-yl)methanone: W35 (≠ Q29), D37 (≠ G31), A38 (≠ Q32), F41 (≠ R35)
binding pyridoxal-5'-phosphate: C117 (≠ G115), F118 (≠ Y116), N121 (= N119), H144 (= H142), E187 (= E185), S191 (= S189), D216 (= D214), V218 (= V216), H219 (= H217), T247 (= T245), K250 (= K248), T279 (= T277), T280 (= T278)

5qqzB Pandda analysis group deposition -- crystal structure of human alas2a in complex with z1675346324
48% identity, 97% coverage: 1:396/408 of query aligns to 7:400/428 of 5qqzB

query
sites
5qqzB

M

F

D

S

Y

Y

K

D

A

Q

A

F

F

F

R

R

S

D

A

K

V

I

E

M

Q

E

I

K

R

K

E

Q

E

D

G

H

R

T

Y

Y

R

R

V

V

F

F

A

K

D

T

L

V

K

N

R

R

Q
x
W

R

A

G

D

Q

A

F

Y

P

P

R

F

A

A

T

-

W

-

T

-

R

-

Q

Q

D

H

G

F

S

S

E

S

H

K

E
x
D

V

V

V

S

V

V

W

W

C

C

S

S

N

N

D

D

Y

Y

L

L

G

G

Q

M

G

S

Q

R

N

H

P

P

V

Q

V

V

L

L

E

Q

A

A

M

T

K

Q

A

E

A

T

V

L

D

Q

E

R

H

H

G

G

S

V

G

G

S

A

G

G

T

T

R

R

N

N

I

I

S

S

G

G

T

T

N

S

H

K

D

F

H

H

V

V

L

E

L

L

E

E

Q

Q

E

E

L

L

A

A

D

E

L

L

H

H

G

Q

K

K

E

D

A

S

G

A

L

L

F

F

T

S

S

S

G
x
C

Y
x
F

V

V

S

A

N
|
N

E

D

A

S

T

T

L

L

S

F

V

T

V

L

Q

A

K

K

I

I

L

L

P

P

G

G

L

C

I

E

I

I

F

Y

S

S

D

D

E

A

L

G

N

N

H
|
H

A

A

S

S

M

M

I

I

A

Q

G

G

I

I

R

R

N

N

G

S

G

G

G

A

P

A

R

K

K

F

I

V

F

F

K

R

H

H

N

N

D

D

L

P

A

D

H

H

L

L

E

K

Q

K

L

L

A

E

E

K

A

S

P

N

A

P

D

K

A

I

P

P

K

K

L

I

I

V

A

A

F

F

E
|
E

S

T

V

V

Y

H

S

S

M

M

D

D

G

G

D

A

I

I

A

C

D

P

L

L

A

E

G

E

T

L

V

C

A

D

L

V

A

S

K

H

K

Q

Y

Y

G

G

A

A

M

L

T

T

Y

F

L

V

D
|
D

E

E

V
|
V

H
|
H

A

A

V

V

G

G

M

L

Y

Y

G

G

P

S

R

R

G

G

G

A

G

G

V

I

A

G

E

E

R

R

D

D

G

G

L

I

M

M

G

H

E

K

I

I

D

D

I

I

E

S

G

G

T

T

L

L

G

G

K
|
K

A

A

F

F

G

G

V

C

M

V

G

G

Y

Y

I

I

T

A

G

S

D

T

A

R

E

D

V

L

I

V

D

D

A

M

I

V

R

R

L

S

M

Y

A

A

S

A

G

G

F

F

I

I

F

F

T
|
T

S

S

L

L

P

P

A

M

L

V

T

L

A

S

G

G

A

A

L

L

A

E

S

S

V

V

R

R

W

L

L

L

K

K

-

G

-

E

Q

E

H

G

P

Q

E

A

V

L

R

R

E

R

I

A

H

H

Q

Q

E

R

R

N

A

V

A

K

T

H

L

M

K

R

A

Q

M

L

F

L

K

M

A

D

A

R

G

G

L

L

P

P

V

V

M

I

D

P

S

C

V

P

S

S

H

H

I

I

V

I

P

P

V

I

L

R

V

V

G

G

D

N

P

A

V

A

H

L

C

N

K

S

M

K

I

L

S

C

D

D

M

L

L

L

A

S

D

K

F

H

G

G

V

I

Y

Y

V

V

Q

Q

P

A

I

I

N

N

Y

Y

P

P

T

T

V

V

P

P

R

R

G

G

T

E

E

E

R

L

L

L

R

R

F

L

T

A

P

P

T

S

P

P

F

H

H

H

T

S

D

P

D

Q

M

M

R

E

K

D

L

F

V

V

A

E

A

K

M

L

E

L

K

L

L

A

W

W

A

T

binding trans-3-[(2,6-dimethylpyrimidin-4-yl)(methyl)amino]cyclobutan-1-ol: W35 (≠ Q29), D49 (≠ E47)
binding pyridoxal-5'-phosphate: C117 (≠ G115), F118 (≠ Y116), N121 (= N119), H144 (= H142), E187 (= E185), D216 (= D214), V218 (= V216), H219 (= H217), K250 (= K248), T279 (= T277), T280 (= T278)

5qqrB Pandda analysis group deposition -- crystal structure of human alas2a in complex with z1171217421
48% identity, 97% coverage: 1:396/408 of query aligns to 7:400/429 of 5qqrB

query
sites
5qqrB

M

F

D

S

Y

Y

K

D

A

Q

A

F

F

F

R

R

S

D

A

K

V

I

E

M

Q

E

I

K

R

K

E

Q

E

D

G

H

R

T

Y

Y

R

R

V

V

F

F

A

K

D

T

L

V

K

N

R

R

Q

W

R

A

G

D

Q

A

F

Y

P

P

R

F

A

A

T

-

W

-

T

-

R

-

Q

Q

D

H

G

F

S

S

E

S

H

K

E

D

V

V

V

S

V

V

W

W

C

C

S

S

N

N

D

D

Y

Y

L

L

G

G

Q

M

G

S

Q

R

N

H

P

P

V

Q

V

V

L

L

E

Q

A

A

M

T

K

Q

A

E

A

T

V

L

D

Q

E

R

H

H

G

G

S

V

G

G

S

A

G

G

T

T

R

R

N

N

I

I

S

S

G

G

T

T

N

S

H

K

D

F

H

H

V

V

L

E

L

L

E

E

Q

Q

E

E

L

L

A

A

D

E

L

L

H

H

G

Q

K

K

E

D

A

S

G

A

L

L

F

F

T

S

S
|
S

G
x
C

Y
x
F

V

V

S

A

N

N

E

D

A

S

T

T

L

L

S

F

V

T

V

L

Q

A

K

K

I

I

L

L

P

P

G

G

L

C

I

E

I

I

F

Y

S

S

D

D

E

A

L

G

N

N

H
|
H

A

A

S

S

M

M

I

I

A

Q

G

G

I

I

R

R

N

N

G

S

G

G

G

A

P

A

R

K

K

F

I

V

F

F

K

R

H

H

N

N

D

D

L

P

A

D

H

H

L

L

E

K

Q

K

L

L

A

E

E

K

A

S

P

N

A

P

D

K

A

I

P

P

K

K

L

I

I

V

A

A

F

F

E
|
E

S

T

V

V

Y

H

S
|
S

M

M

D

D

G

G

D

A

I

I

A

C

D

P

L

L

A

E

G

E

T

L

V

C

A

D

L

V

A

S

K

H

K

Q

Y

Y

G

G

A

A

M

L

T

T

Y

F

L

V

D
|
D

E

E

V
|
V

H
|
H

A

A

V

V

G

G

M

L

Y

Y

G

G

P

S

R

R

G

G

G

A

G

G

V

I

A

G

E

E

R

R

D

D

G

G

L

I

M

M

G

H

E

K

I

I

D

D

I

I

E

S

G

G

T
|
T

L

L

G

G

K
|
K

A

A

F

F

G

G

V

C

M

V

G

G

Y

Y

I

I

T

A

G

S

D

T

A

R

E

D

V

L

I

V

D

D

A

M

I

V

R

R

L

S

M

Y

A

A

S

A

G

G

F

F

I

I

F

F

T
|
T

S

S

L

L

P

P

A

M

L

V

T

L

A

S

G

G

A

A

L

L

A

E

S

S

V

V

R

R

W

L

L

L

K

K

-

G

-

E

Q

E

H

G

P

Q

E

A

V

L

R

R

E

R

I

A

H

H

Q

Q

E

R

R

N

A

V

A

K

T

H

L

M

K

R

A

Q

M

L

F

L

K

M

A

D

A

R

G

G

L

L

P

P

V

V

M

I

D

P

S

C

V

P

S

S

H

H

I

I

V

I

P

P

V

I

L

R

V

V

G

G

D

N

P

A

V

A

H

L

C

N

K

S

M

K

I

L

S

C

D

D

M

L

L

L

A

S

D

K

F

H

G

G

V

I

Y

Y

V

V

Q

Q

P

A

I

I

N

N

Y

Y

P

P

T

T

V

V

P

P

R

R

G

G

T

E

E

E

R

L

L

L

R

R

F

L

T

A

P

P

T

S

P

P

F

H

H

H

T

S

D

P

D

Q

M

M

R

E

K

D

L

F

V

V

A

E

A

K

M

L

E

L

K

L

L

A

W

W

A

T

binding pyridoxal-5'-phosphate: S116 (= S114), C117 (≠ G115), F118 (≠ Y116), H144 (= H142), E187 (= E185), S191 (= S189), D216 (= D214), V218 (= V216), H219 (= H217), T247 (= T245), K250 (= K248), T279 (= T277), T280 (= T278)

Sites not aligning to the query:

binding 1-[(2-methyl-1,3-thiazol-5-yl)methyl]piperazine: 407, 408, 409

P08680 5-aminolevulinate synthase, erythroid-specific, mitochondrial; ALAS-E; 5-aminolevulinic acid synthase 2; Delta-ALA synthase 2; Delta-aminolevulinate synthase 2; EC 2.3.1.37 from Mus musculus (Mouse) (see 2 papers)
48% identity, 96% coverage: 3:395/408 of query aligns to 145:540/587 of P08680

query
sites
P08680

Y

Y

K

D

A

Q

A

F

F

F

R

R

S

D

A

K

V

I

E

M

Q

E

I

K

R

K

E

Q

E

D

G

H

R

T

Y

Y

R

R

V

V

F

F

A

K

D

T

L

V

K

N

R

R

Q

W

R

A

G

N

Q

A

F

Y

P

P

R

F

A

A

T

Q

-

H

W

F

T

S

R

E

Q

A

D

S

G

M

S

A

E

S

H

K

E

D

V

V

V

S

V

V

W

W

C

C

S

S

N

N

D

D

Y

Y

L

L

G

G

Q

I

G

S

Q

R

N

H

P

P

V

R

V

V

L

L

E

Q

A

A

M

I

K

E

A

E

A

T

V

L

D

K

E

N

H

H

G

G

S

A

G

G

S

A

G

G

T

T

R

R

N

N

I

I

S

S

G

G

T

T

N

S

H

K

D

F

H

H

V

V

L

E

L

L

E

E

Q

Q

E

E

L

L

A

A

D

E

L

L

H

H

G

Q

K

K

E

D

A

S

G

A

L

L

F

F

T

S

S

S

G

C

Y

F

V

V

S

A

N

N

E

D

A

S

T

T

L

L

S

F

V

T

V

L

Q

A

K

K

I

L

L

L

P

P

G

G

L

C

I

E

I

I

F

Y

S

S

D

D

E

A

L

G

N

N

H

H

A

A

S

S

M

M

I

I

A

Q

G

G

I

I

R

R

N

N

G

S

G

G

G

A

P

A

R

K

K

F

I

V

F

F

K

R

H

H

N

N

D

D

L

P

A

G

H

H

L

L

E

K

Q

K

L

L

A

E

E

K

A

S

P

D

A

P

D

K

A

T

P

P

K

K

L

I

I

V

A

A

F

F

E

E

S

T

V

V

Y

H

S

S

M

M

D

D

G

G

D

A

I

I

A

C

D

P

L

L

A

E

G

E

T

L

V

C

A

D

L

V

A

A

K

H

K

Q

Y

Y

G

G

A

A

M

L

T

T

Y

F

L

V

D

D

E

E

V

V

H

H

A

A

V

V

G

G

M

L

Y

Y

G

G

P

A

R

R

G

G

G

A

G

G

V

I

A

G

E

E

R

R

D

D

G

G

L

I

M

M

G

H

E

K

I

L

D

D

I

I

E

S

G

G

T

T

L

L

G

G

K
|
K

A

A

F

F

G

G

V

C

M

V

G

G

Y

Y

I

I

T

A

G

S

D

T

A

R

E

D

V

L

I

V

D

D

A

M

I

V

R

R

L

S

M

Y

A

A

S

A

G

G

F

F

I

I

F

F

T

T

S

S

L

L

P

P

A

M

L

V

T

L

A

S

G

G

A

A

L

L

A

E

S

S

V

V

R

R

W

L

L

L

K

K

-

G

-

E

Q

E

H

G

P

Q

E

A

V

L

R

R

E

R

I

A

H

H

Q

Q

E

R

R

N

A

V

A

K

T

H

L

M

K

R

A

Q

M

L

F

L

K

M

A

D

A

R

G

G

L

F

P

P

V

V

M

I

D

P

S

C

V

P

S

S

H

H

I

I

V

I

P

P

V

I

L

R

V

V

G

G

D

N

P

A

V

A

H

L

C

N

K

S

M

K

I

I

S

C

D

D

M

L

L

L

A

S

D

K

F

H

G

S

V

I

Y

Y

V

V

Q

Q

P

A

I

I

N

N

Y

Y

P

P

T

T

V

V

P

P

R

R

G

G

T

E

E

E

R

L

L

L

R

R

F

L

T

A

P

P

T

S

P

P

F

H

H

H

T

S

D

P

D

Q

M

M

R

E

K

N

L

F

V

V

A

E

A

K

M

L

E

L

K

L

L

A

W

W

K391 (= K248) modified: N6-(pyridoxal phosphate)lysine; mutation K->A,H: Abolishes enzyme activity.

Sites not aligning to the query:

1:49 modified: transit peptide, Mitochondrion

6hrhA Structure of human erythroid-specific 5'-aminolevulinate synthase, alas2
48% identity, 97% coverage: 1:396/408 of query aligns to 7:399/429 of 6hrhA

query
sites
6hrhA

M

F

D

S

Y

Y

K

D

A

Q

A

F

F

F

R

R

S

D

A

K

V

I

E

M

Q

E

I

K

R

K

E

Q

E

D

G

H

R

T

Y

Y

R

R

V

V

F

F

A

K

D

T

L

V

K

N

R

R

Q

W

R

A

G

D

Q

A

F

Y

P

P

R

F

A

A

T

Q

W

H

T

F

R

S

Q

K

D

D

G

-

S

-

E

-

H

-

E

-

V

V

V

S

V

V

W

W

C

C

S

S

N

N

D

D

Y

Y

L

L

G

G

Q

M

G

S

Q

R

N

H

P

P

V

Q

V

V

L

L

E

Q

A

A

M

T

K

Q

A

E

A

T

V

L

D

Q

E

R

H

H

G

G

S

V

G

G

S

A

G

G

T

T

R

R

N

N

I

I

S

S

G

G

T

T

N

S

H

K

D

F

H

H

V

V

L

E

L

L

E

E

Q

Q

E

E

L

L

A

A

D

E

L

L

H

H

G

Q

K

K

E

D

A

S

G

A

L

L

F

F

T

S

S
|
S

G
x
C

Y
x
F

V

V

S

A

N

N

E

D

A

S

T

T

L

L

S

F

V

T

V

L

Q

A

K

K

I

I

L

L

P

P

G

G

L

C

I

E

I

I

F

Y

S

S

D

D

E

A

L

G

N

N

H
|
H

A

A

S

S

M

M

I

I

A

Q

G

G

I

I

R

R

N

N

G

S

G

G

G

A

P

A

R

K

K

F

I

V

F

F

K

R

H

H

N

N

D

D

L

P

A

D

H

H

L

L

E

K

Q

K

L

L

A

E

E

K

A

S

P

N

A

P

D

K

A

I

P

P

K

K

L

I

I

V

A

A

F

F

E
|
E

S

T

V

V

Y

H

S
|
S

M

M

D

D

G

G

D

A

I

I

A

C

D

P

L

L

A

E

G

E

T

L

V

C

A

D

L

V

A

S

K

H

K

Q

Y

Y

G

G

A

A

M

L

T

T

Y

F

L

V

D
|
D

E

E

V

V

H
|
H

A

A

V

V

G

G

M

L

Y

Y

G

G

P

S

R

R

G

G

G

A

G

G

V

I

A

G

E

E

R

R

D

D

G

G

L

I

M

M

G

H

E

K

I

I

D

D

I

I

E

S

G

G

T
|
T

L

L

G

G

K
|
K

A

A

F

F

G

G

V

C

M

V

G

G

Y

Y

I

I

T

A

G

S

D

T

A

R

E

D

V

L

I

V

D

D

A

M

I

V

R

R

L

S

M

Y

A

A

S

A

G

G

F

F

I

I

F

F

T
|
T

S

S

L

L

P

P

A

M

L

V

T

L

A

S

G

G

A

A

L

L

A

E

S

S

V

V

R

R

W

L

L

L

K

K

-

G

-

E

Q

E

H

G

P

Q

E

A

V

L

R

R

E

R

I

A

H

H

Q

Q

E

R

R

N

A

V

A

K

T

H

L

M

K

R

A

Q

M

L

F

L

K

M

A

D

A

R

G

G

L

L

P

P

V

V

M

I

D

P

S

C

V

P

S

S

H

H

I

I

V

I

P

P

V

I

L

R

V

V

G

G

D

N

P

A

V

A

H

L

C

N

K

S

M

K

I

L

S

C

D

D

M

L

L

L

A

S

D

K

F

H

G

G

V

I

Y

Y

V

V

Q

Q

P

A

I

I

N

N

Y

Y

P

P

T

T

V

V

P

P

R

R

G

G

T

E

E

E

R

L

L

L

R

R

F

L

T

A

P

P

T

S

P

P

F

H

H

H

T

S

D

P

D

Q

M

M

R

E

K

D

L

F

V

V

A

E

A

K

M

L

E

L

K

L

L

A

W

W

A

T

binding pyridoxal-5'-phosphate: S115 (= S114), C116 (≠ G115), F117 (≠ Y116), H143 (= H142), E186 (= E185), S190 (= S189), D215 (= D214), H218 (= H217), T246 (= T245), K249 (= K248), T278 (= T277), T279 (= T278)

5qreA Pandda analysis group deposition -- crystal structure of human alas2a in complex with z117233350
48% identity, 97% coverage: 1:396/408 of query aligns to 7:399/429 of 5qreA

query
sites
5qreA

M

F

D

S

Y

Y

K

D

A

Q

A

F

F

F

R

R

S

D

A

K

V

I

E

M

Q

E

I

K

R

K

E

Q

E

D

G

H

R

T

Y

Y

R

R

V

V

F

F

A

K

D

T

L

V

K

N

R

R

Q

W

R

A

G

D

Q

A

F

Y

P

P

R

F

A

A

T

Q

W

H

T

F

R

S

Q

K

D

D

G

-

S

-

E

-

H

-

E

-

V

V

V

S

V

V

W

W

C

C

S

S

N

N

D

D

Y

Y

L

L

G

G

Q

M

G

S

Q

R

N

H

P

P

V

Q

V

V

L

L

E

Q

A

A

M

T

K

Q

A

E

A

T

V

L

D

Q

E

R

H

H

G

G

S

V

G

G

S

A

G

G

T

T

R

R

N

N

I

I

S

S

G

G

T

T

N

S

H

K

D

F

H

H

V

V

L

E

L

L

E

E

Q

Q

E

E

L

L

A

A

D

E

L

L

H

H

G

Q

K

K

E

D

A

S

G

A

L

L

F

F

T

S

S

S

G
x
C

Y
x
F

V

V

S

A

N

N

E

D

A

S

T

T

L

L

S

F

V

T

V

L

Q

A

K

K

I

I

L

L

P

P

G

G

L

C

I

E

I

I

F

Y

S

S

D

D

E

A

L

G

N

N

H
|
H

A

A

S

S

M

M

I

I

A

Q

G

G

I

I

R

R

N

N

G

S

G

G

G

A

P

A

R

K

K

F

I

V

F

F

K

R

H

H

N

N

D

D

L

P

A

D

H

H

L

L

E

K

Q

K

L

L

A

E

E

K

A

S

P

N

A

P

D

K

A

I

P

P

K

K

L

I

I

V

A

A

F

F

E
|
E

S

T

V

V

Y

H

S
|
S

M

M

D

D

G

G

D

A

I

I

A

C

D

P

L

L

A

E

G

E

T

L

V

C

A

D

L

V

A

S

K

H

K

Q

Y

Y

G

G

A

A

M

L

T

T

Y

F

L

V

D
|
D

E

E

V
|
V

H
|
H

A

A

V

V

G

G

M

L

Y

Y

G

G

P

S

R

R

G

G

G

A

G

G

V

I

A

G

E

E

R

R

D

D

G

G

L

I

M

M

G

H

E

K

I

I

D

D

I

I

E

S

G

G

T
|
T

L

L

G

G

K
|
K

A

A

F

F

G

G

V

C

M

V

G

G

Y

Y

I

I

T

A

G

S

D

T

A

R

E

D

V

L

I

V

D

D

A

M

I

V

R

R

L

S

M

Y

A

A

S

A

G

G

F

F

I

I

F

F

T
|
T

S

S

L

L

P

P

A

M

L

V

T

L

A

S

G

G

A

A

L

L

A

E

S

S

V

V

R

R

W

L

L

L

K

K

-

G

-

E

Q

E

H

G

P

Q

E

A

V

L

R

R

E

R

I

A

H

H

Q

Q

E

R

R

N

A

V

A

K

T

H

L

M

K

R

A

Q

M

L

F

L

K

M

A

D

A

R

G

G

L

L

P

P

V

V

M

I

D

P

S

C

V

P

S

S

H

H

I

I

V

I

P

P

V

I

L

R

V

V

G

G

D

N

P

A

V

A

H

L

C

N

K

S

M

K

I

L

S

C

D

D

M

L

L

L

A

S

D

K

F

H

G

G

V

I

Y

Y

V

V

Q

Q

P

A

I

I

N

N

Y

Y

P

P

T

T

V

V

P

P

R

R

G

G

T

E

E

E

R

L

L

L

R

R

F

L

T

A

P

P

T

S

P

P

F

H

H

H

T

S

D

P

D

Q

M

M

R

E

K

D

L

F

V

V

A

E

A

K

M

L

E

L

K

L

L

A

W

W

A

T

binding pyridoxal-5'-phosphate: C116 (≠ G115), F117 (≠ Y116), H143 (= H142), E186 (= E185), S190 (= S189), D215 (= D214), V217 (= V216), H218 (= H217), T246 (= T245), K249 (= K248), T278 (= T277), T279 (= T278)

Sites not aligning to the query:

binding 3-ethyl-5-methyl-N-(5-methyl-1,2-oxazol-3-yl)-1,2-oxazole-4-carboxamide: 424, 425

5qrcA Pandda analysis group deposition -- crystal structure of human alas2a in complex with z31721798
48% identity, 97% coverage: 1:396/408 of query aligns to 7:399/429 of 5qrcA

query
sites
5qrcA

M

F

D

S

Y

Y

K

D

A

Q

A

F

F

F

R

R

S

D

A

K

V

I

E

M

Q

E

I

K

R

K

E

Q

E

D

G

H

R

T

Y

Y

R

R

V

V

F

F

A

K

D

T

L

V

K

N

R

R

Q

W

R

A

G

D

Q

A

F

Y

P

P

R

F

A

A

T

Q

W

H

T

F

R

S

Q

K

D

D

G

-

S

-

E

-

H

-

E

-

V

V

V

S

V

V

W

W

C

C

S

S

N

N

D

D

Y

Y

L

L

G

G

Q

M

G

S

Q

R

N

H

P

P

V

Q

V

V

L

L

E

Q

A

A

M

T

K

Q

A

E

A

T

V

L

D

Q

E

R

H

H

G

G

S

V

G

G

S

A

G

G

T

T

R

R

N

N

I

I

S

S

G

G

T

T

N

S

H

K

D

F

H

H

V

V

L

E

L

L

E

E

Q

Q

E

E

L

L

A

A

D

E

L

L

H

H

G

Q

K

K

E

D

A

S

G

A

L

L

F

F

T

S

S
|
S

G
x
C

Y
x
F

V

V

S

A

N

N

E

D

A

S

T

T

L

L

S

F

V

T

V

L

Q

A

K
|
K

I
|
I

L

L

P

P

G

G

L

C

I

E

I

I

F

Y

S

S

D

D

E

A

L

G

N

N

H
|
H

A

A

S

S

M

M

I

I

A

Q

G

G

I

I

R

R

N

N

G

S

G

G

G

A

P

A

R

K

K

F

I

V

F

F

K

R

H

H

N

N

D

D

L

P

A

D

H

H

L

L

E

K

Q

K

L

L

A

E

E

K

A

S

P

N

A

P

D

K

A

I

P

P

K

K

L

I

I

V

A

A

F

F

E
|
E

S

T

V

V

Y

H

S
|
S

M

M

D

D

G

G

D

A

I

I

A

C

D

P

L

L

A

E

G

E

T

L

V

C

A

D

L

V

A

S

K

H

K

Q

Y

Y

G

G

A

A

M

L

T

T

Y

F

L

V

D
|
D

E

E

V
|
V

H
|
H

A

A

V

V

G

G

M

L

Y

Y

G

G

P

S

R

R

G

G

G

A

G

G

V

I

A

G

E

E

R

R

D

D

G

G

L

I

M

M

G

H

E

K

I

I

D

D

I

I

E

S

G

G

T

T

L

L

G

G

K
|
K

A

A

F

F

G

G

V

C

M

V

G

G

Y

Y

I

I

T

A

G

S

D

T

A

R

E

D

V

L

I

V

D

D

A

M

I

V

R

R

L

S

M
x
Y

A

A

S

A

G

G

F

F

I

I

F

F

T
|
T

S

S

L

L

P

P

A

M

L

V

T

L

A

S

G

G

A

A

L

L

A

E

S

S

V

V

R

R

W

L

L

L

K

K

-

G

-

E

Q

E

H

G

P

Q

E

A

V

L

R

R

E

R

I

A

H

H

Q

Q

E

R

R

N

A

V

A

K

T

H

L

M

K

R

A

Q

M

L

F

L

K

M

A

D

A

R

G

G

L

L

P

P

V

V

M

I

D

P

S

C

V

P

S

S

H

H

I

I

V

I

P

P

V

I

L

R

V

V

G

G

D

N

P

A

V

A

H

L

C

N

K

S

M

K

I

L

S

C

D

D

M

L

L

L

A

S

D

K

F

H

G

G

V

I

Y

Y

V

V

Q

Q

P

A

I

I

N

N

Y

Y

P

P

T

T

V

V

P

P

R

R

G

G

T

E

E

E

R

L

L

L

R

R

F

L

T

A

P

P

T

S

P

P

F

H

H

H

T

S

D

P

D

Q

M

M

R

E

K

D

L

F

V

V

A

E

A

K

M

L

E

L

K

L

L

A

W

W

A

T

binding 3-cyclohexyl-1-(morpholin-4-yl)propan-1-one: K129 (= K128), I130 (= I129), Y271 (≠ M270)
binding pyridoxal-5'-phosphate: S115 (= S114), C116 (≠ G115), F117 (≠ Y116), H143 (= H142), E186 (= E185), S190 (= S189), D215 (= D214), V217 (= V216), H218 (= H217), K249 (= K248), T278 (= T277), T279 (= T278)

5qraA Pandda analysis group deposition -- crystal structure of human alas2a in complex with z1101755952
48% identity, 97% coverage: 1:396/408 of query aligns to 7:399/429 of 5qraA

query
sites
5qraA

M

F

D

S

Y

Y

K

D

A

Q

A

F

F

F

R

R

S

D

A

K

V

I

E

M

Q

E

I

K

R

K

E

Q

E

D

G

H

R

T

Y

Y

R

R

V

V

F

F

A

K

D

T

L

V

K

N

R

R

Q

W

R

A

G

D

Q

A

F

Y

P

P

R

F

A

A

T

Q

W

H

T

F

R

S

Q

K

D

D

G

-

S

-

E

-

H

-

E

-

V

V

V

S

V

V

W

W

C

C

S

S

N

N

D

D

Y

Y

L

L

G

G

Q

M

G

S

Q

R

N

H

P

P

V

Q

V

V

L

L

E

Q

A

A

M

T

K

Q

A

E

A

T

V

L

D

Q

E

R

H

H

G

G

S

V

G

G

S

A

G

G

T

T

R

R

N

N

I

I

S

S

G

G

T

T

N

S

H

K

D

F

H

H

V

V

L

E

L

L

E

E

Q

Q

E

E

L

L

A

A

D

E

L

L

H

H

G

Q

K

K

E

D

A

S

G

A

L

L

F

F

T

S

S
|
S

G
x
C

Y
x
F

V

V

S

A

N

N

E

D

A

S

T

T

L

L

S

F

V

T

V

L

Q

A

K
|
K

I
|
I

L

L

P

P

G

G

L

C

I

E

I

I

F

Y

S

S

D

D

E

A

L

G

N

N

H
|
H

A

A

S

S

M

M

I

I

A

Q

G

G

I

I

R

R

N

N

G

S

G

G

G

A

P

A

R

K

K

F

I

V

F

F

K

R

H

H

N

N

D

D

L

P

A

D

H

H

L

L

E

K

Q

K

L

L

A

E

E

K

A

S

P

N

A

P

D

K

A

I

P

P

K

K

L

I

I

V

A

A

F

F

E
|
E

S

T

V

V

Y

H

S
|
S

M

M

D

D

G

G

D

A

I

I

A

C

D

P

L

L

A

E

G

E

T

L

V

C

A

D

L

V

A

S

K

H

K

Q

Y

Y

G

G

A

A

M

L

T

T

Y

F

L

V

D
|
D

E

E

V
|
V

H
|
H

A

A

V

V

G

G

M

L

Y

Y

G

G

P

S

R

R

G

G

G

A

G

G

V

I

A

G

E

E

R

R

D

D

G

G

L

I

M

M

G

H

E

K

I

I

D

D

I

I

E

S

G

G

T
|
T

L

L

G

G

K
|
K

A

A

F

F

G

G

V

C

M

V

G

G

Y

Y

I

I

T

A

G

S

D

T

A

R

E

D

V

L

I

V

D

D

A

M

I

V

R

R

L

S

M
x
Y

A

A

S

A

G

G

F

F

I

I

F

F

T
|
T

S

S

L

L

P

P

A

M

L

V

T

L

A

S

G

G

A

A

L

L

A

E

S

S

V

V

R

R

W

L

L

L

K

K

-

G

-

E

Q

E

H

G

P

Q

E

A

V

L

R

R

E

R

I

A

H

H

Q

Q

E

R

R

N

A

V

A

K

T

H

L

M

K

R

A

Q

M

L

F

L

K

M

A

D

A

R

G

G

L

L

P

P

V

V

M

I

D

P

S

C

V

P

S

S

H

H

I

I

V

I

P

P

V

I

L

R

V

V

G

G

D

N

P

A

V

A

H

L

C

N

K

S

M

K

I

L

S

C

D

D

M

L

L

L

A

S

D

K

F

H

G

G

V

I

Y

Y

V

V

Q

Q

P

A

I

I

N

N

Y

Y

P

P

T

T

V

V

P

P

R

R

G

G

T

E

E

E

R

L

L

L

R

R

F

L

T

A

P

P

T

S

P

P

F

H

H

H

T

S

D

P

D

Q

M

M

R

E

K

D

L

F

V

V

A

E

A

K

M

L

E

L

K

L

L

A

W

W

A

T

binding [(4R)-4-methyl-2,3,4,5-tetrahydro-1H-azepin-1-yl](1,3-thiazol-4-yl)methanone: K129 (= K128), I130 (= I129), Y271 (≠ M270)
binding pyridoxal-5'-phosphate: S115 (= S114), C116 (≠ G115), F117 (≠ Y116), H143 (= H142), E186 (= E185), S190 (= S189), D215 (= D214), V217 (= V216), H218 (= H217), T246 (= T245), K249 (= K248), T278 (= T277), T279 (= T278)

5qr9A Pandda analysis group deposition -- crystal structure of human alas2a in complex with z31478129
48% identity, 97% coverage: 1:396/408 of query aligns to 7:399/429 of 5qr9A

query
sites
5qr9A

M

F

D

S

Y

Y

K

D

A

Q

A

F

F

F

R

R

S

D

A

K

V

I

E

M

Q

E

I

K

R

K

E

Q

E

D

G

H

R

T

Y

Y

R

R

V

V

F

F

A

K

D

T

L

V

K

N

R

R

Q

W

R

A

G

D

Q

A

F

Y

P

P

R

F

A

A

T

Q

W

H

T

F

R

S

Q

K

D

D

G

-

S

-

E

-

H

-

E

-

V

V

V

S

V

V

W

W

C

C

S

S

N

N

D

D

Y

Y

L

L

G

G

Q

M

G

S

Q

R

N

H

P

P

V

Q

V

V

L

L

E

Q

A

A

M

T

K

Q

A

E

A

T

V

L

D

Q

E

R

H

H

G

G

S

V

G

G

S

A

G

G

T

T

R

R

N

N

I

I

S

S

G

G

T

T

N

S

H

K

D

F

H

H

V

V

L

E

L

L

E

E

Q

Q

E

E

L

L

A

A

D

E

L

L

H

H

G

Q

K

K

E

D

A

S

G

A

L

L

F

F

T

S

S
|
S

G
x
C

Y
x
F

V

V

S

A

N

N

E

D

A

S

T

T

L

L

S

F

V

T

V

L

Q

A

K

K

I

I

L

L

P

P

G

G

L

C

I

E

I

I

F

Y

S

S

D

D

E

A

L

G

N

N

H
|
H

A

A

S

S

M

M

I

I

A

Q

G

G

I

I

R

R

N

N

G

S

G

G

G

A

P

A

R

K

K

F

I

V

F

F

K

R

H

H

N

N

D

D

L

P

A

D

H

H

L

L

E

K

Q

K

L

L

A

E

E

K

A

S

P

N

A

P

D

K

A

I

P

P

K

K

L

I

I

V

A

A

F

F

E
|
E

S

T

V

V

Y

H

S
|
S

M

M

D

D

G

G

D

A

I

I

A

C

D

P

L

L

A

E

G

E

T

L

V

C

A

D

L

V

A

S

K
x
H

K

Q

Y

Y

G

G

A

A

M
x
L

T

T

Y

F

L

V

D
|
D

E

E

V
|
V

H
|
H

A

A

V

V

G

G

M

L

Y

Y

G

G

P

S

R

R

G

G

G

A

G

G

V

I

A

G

E

E

R

R

D

D

G

G

L

I

M

M

G

H

E

K

I

I

D
|
D

I

I

E

S

G

G

T

T

L

L

G

G

K
|
K

A

A

F

F

G

G

V

C

M

V

G

G

Y

Y

I

I

T

A

G

S

D

T

A

R

E

D

V

L

I

V

D

D

A

M

I

V

R

R

L

S

M

Y

A

A

S

A

G

G

F

F

I

I

F

F

T
|
T

S

S

L

L

P

P

A

M

L

V

T

L

A

S

G

G

A

A

L

L

A

E

S

S

V

V

R

R

W

L

L

L

K

K

-

G

-

E

Q

E

H

G

P

Q

E

A

V

L

R

R

E

R

I

A

H

H

Q

Q

E

R

R

N

A

V

A

K

T

H

L

M

K

R

A

Q

M

L

F

L

K

M

A

D

A

R

G

G

L

L

P

P

V

V

M

I

D

P

S

C

V

P

S

S

H

H

I

I

V

I

P

P

V

I

L

R

V

V

G

G

D

N

P

A

V

A

H

L

C

N

K

S

M

K

I

L

S

C

D

D

M

L

L

L

A

S

D

K

F

H

G

G

V

I

Y

Y

V

V

Q

Q

P

A

I

I

N

N

Y

Y

P

P

T

T

V

V

P

P

R

R

G

G

T

E

E

E

R

L

L

L

R

R

F

L

T

A

P

P

T

S

P

P

F

H

H

H

T

S

D

P

D

Q

M

M

R

E

K

D

L

F

V

V

A

E

A

K

M

L

E

L

K

L

L

A

W

W

A

T

binding ethyl benzylcarbamate: H206 (≠ K205), L211 (≠ M210), D241 (= D240)
binding pyridoxal-5'-phosphate: S115 (= S114), C116 (≠ G115), F117 (≠ Y116), H143 (= H142), E186 (= E185), S190 (= S189), D215 (= D214), V217 (= V216), H218 (= H217), K249 (= K248), T278 (= T277), T279 (= T278)

5qr3A Pandda analysis group deposition -- crystal structure of human alas2a in complex with z915492990
48% identity, 97% coverage: 1:396/408 of query aligns to 7:399/429 of 5qr3A

query
sites
5qr3A

M
x
F

D

S

Y

Y

K

D

A

Q

A

F

F

F

R

R

S

D

A

K

V

I

E

M

Q

E

I

K

R

K

E

Q

E

D

G

H

R

T

Y

Y

R

R

V

V

F

F

A

K

D

T

L

V

K

N

R

R

Q

W

R

A

G

D

Q

A

F

Y

P

P

R

F

A

A

T

Q

W

H

T

F

R

S

Q

K

D

D

G

-

S

-

E

-

H

-

E

-

V

V

V

S

V

V

W

W

C

C

S

S

N

N

D

D

Y

Y

L

L

G

G

Q

M

G

S

Q

R

N

H

P

P

V

Q

V

V

L

L

E

Q

A

A

M

T

K

Q

A

E

A

T

V

L

D

Q

E

R

H

H

G

G

S

V

G

G

S

A

G

G

T

T

R

R

N

N

I

I

S

S

G

G

T

T

N

S

H

K

D

F

H

H

V

V

L

E

L

L

E

E

Q

Q

E

E

L

L

A

A

D

E

L

L

H

H

G

Q

K

K

E

D

A

S

G

A

L

L

F

F

T

S

S
|
S

G
x
C

Y
x
F

V

V

S

A

N

N

E

D

A

S

T

T

L

L

S

F

V

T

V

L

Q

A

K

K

I

I

L

L

P

P

G

G

L

C

I

E

I

I

F

Y

S

S

D

D

E

A

L

G

N

N

H
|
H

A

A

S

S

M

M

I

I

A

Q

G

G

I

I

R

R

N

N

G

S

G

G

G

A

P

A

R

K

K

F

I

V

F

F

K

R

H

H

N

N

D

D

L

P

A

D

H

H

L

L

E

K

Q

K

L

L

A

E

E

K

A

S

P

N

A

P

D

K

A

I

P

P

K

K

L

I

I

V

A

A

F

F

E
|
E

S

T

V

V

Y

H

S
|
S

M

M

D

D

G

G

D

A

I

I

A

C

D

P

L

L

A

E

G

E

T

L

V

C

A

D

L

V

A

S

K

H

K

Q

Y

Y

G

G

A

A

M

L

T

T

Y

F

L

V

D
|
D

E

E

V
|
V

H
|
H

A

A

V

V

G

G

M

L

Y

Y

G

G

P

S

R

R

G

G

G

A

G

G

V

I

A

G

E

E

R

R

D

D

G

G

L

I

M

M

G

H

E

K

I

I

D

D

I

I

E

S

G

G

T

T

L

L

G

G

K
|
K

A

A

F

F

G

G

V

C

M

V

G

G

Y

Y

I

I

T

A

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binding 1-methyl-N-[(thiophen-2-yl)methyl]-1H-pyrazole-5-carboxamide: F7 (≠ M1)
binding pyridoxal-5'-phosphate: S115 (= S114), C116 (≠ G115), F117 (≠ Y116), H143 (= H142), E186 (= E185), S190 (= S189), D215 (= D214), V217 (= V216), H218 (= H217), K249 (= K248), T278 (= T277), T279 (= T278)

Sites not aligning to the query:

binding 1-methyl-N-[(thiophen-2-yl)methyl]-1H-pyrazole-5-carboxamide: 6

5qr1A Pandda analysis group deposition -- crystal structure of human alas2a in complex with z396380540
48% identity, 97% coverage: 1:396/408 of query aligns to 7:399/429 of 5qr1A

query
sites
5qr1A

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binding ~{N}-(cyclobutylmethyl)-1,5-dimethyl-pyrazole-4-carboxamide: I130 (= I129), Y271 (≠ M270)
binding pyridoxal-5'-phosphate: S115 (= S114), C116 (≠ G115), F117 (≠ Y116), H143 (= H142), E186 (= E185), S190 (= S189), D215 (= D214), V217 (= V216), H218 (= H217), T246 (= T245), K249 (= K248), T278 (= T277), T279 (= T278)

Query Sequence

>CCNA_01417 FitnessBrowser__Caulo:CCNA_01417
MDYKAAFRSAVEQIREEGRYRVFADLKRQRGQFPRATWTRQDGSEHEVVVWCSNDYLGQG
QNPVVLEAMKAAVDEHGSGSGGTRNISGTNHDHVLLEQELADLHGKEAGLLFTSGYVSNE
ATLSVVQKILPGLIIFSDELNHASMIAGIRNGGGPRKIFKHNDLAHLEQLLAEAPADAPK
LIAFESVYSMDGDIADLAGTVALAKKYGAMTYLDEVHAVGMYGPRGGGVAERDGLMGEID
IIEGTLGKAFGVMGGYITGDAEVIDAIRLMASGFIFTTSLPPALTAGALASVRWLKQHPE
VREIHQERAATLKAMFKAAGLPVMDSVSHIVPVLVGDPVHCKMISDMLLADFGVYVQPIN
YPTVPRGTERLRFTPTPFHTDDMMRKLVAAMEKLWAHCNVARMGGYAA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory