SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing CCNA_01488 FitnessBrowser__Caulo:CCNA_01488 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 12 hits to proteins with known functional sites (download)

7m3kA Crystal structure of galactonate dehydratase from brucella melitensis biovar abortus 2308
80% identity, 98% coverage: 11:596/600 of query aligns to 2:587/587 of 7m3kA

query
sites
7m3kA

L

L

R

R

S

S

R

R

A

A

W

W

F

F

D

D

N

N

P

P

D

D

N

D

I

V

D

D

M

M

T

T

A

A

L

L

Y

Y

L

L

E

E

R

R

Y

Y

L

M

N

N

F

Y

G

G

L

L

T

S

L

Q

E

E

E

E

L

L

Q

Q

S

S

G

G

K

R

P

P

I

I

I

I

G

G

I

I

A

A

Q

Q

T

T

G

G

S

S

D

D

L

L

S

S

P

P

C
|
C

N

N

R

R

H

H

H

H

L

L

V

E

L

L

A

A

E

K

R

R

V

V

R

R

E

D

G

G

I

V

R

R

S

E

A

A

G

G

G

G

I

I

A

V

L

I

E

E

F

F

P

P

V

V

H

H

P

P

I

I

Q

Q

E

E

T

T

G

G

K

K

R

R

P

P

T

T

A

A

G

G

L

L

D

D

R

R

N

N

L

L

S

A

Y

Y

L

L

G

G

L

L

V

V

E

E

L

V

L

L

Y

Y

G

G

Y

Y

P

P

L

L

D

D

G

G

V

V

V

V

L

L

T

T

I

I

G

G

C
|
C

D

D

K

K

T

T

T

T

P

P

A

A

C

C

L

L

M

M

A

A

A

A

A

A

T

T

V

V

N

N

I

I

P

P

A

A

I

I

A

A

L

L

S

S

V

V

G

G

P

P

M

M

L

L

N

N

G

G

W

W

H

F

K

R

G

G

K

E

R

R

T

T

G

G

S

S

G

G

T

T

I

I

V

V

W

W

K

K

A

A

R

R

E

E

M

L

L

L

A

A

A

K

G

G

E

E

I

I

D

D

N

Y

A

Q

G

G

F

F

I

V

K

K

L

L

V

V

A

A

S

S

S

S

A

A

P

P

S

S

T

T

G

G

Y

Y

C

C

N

N

T

T

M

M

G

G

T

T

A

A

T

T

T

T

M

M

N

N

S

S

L

L

T

A

E

E

A

A

L

L

G

G

M

M

S

Q

L

L

P

P

G

G

S

S

A

A

A

A

I

I

P

P

A

A

P

P

Y

Y

R

R

D

D

R

R

Q

Q

E

E

N

V

A

A

Y

Y

R

L

T

M

G

G

L

R

R

R

I

I

V

V

E

E

M

M

V

V

A

H

E

E

D

D

L

L

K

K

P

P

S

S

D

D

I

I

L

L

T

T

R

K

E

E

A

A

F

F

L

I

N

N

A

A

I

I

V

R

V

V

N

N

S

S

A

A

I

I

G

G

G

G

S

S

T

T

N

N

A

A

P

P

I

I

H

H

L

L

N

N

A

A

L

L

A

A

R

R

H

H

M

I

D

G

V

V

D

E

L

L

T

T

L

V

E

D

D

D

W

W

E

Q

T

K

A

Y

G

G

K

E

D

E

V

I

P

P

L

L

L

L

V

V

N

N

L

L

Q

Q

P

P

A

A

G

G

E

E

Y

Y

L

L

G

G

E

E

D

D

Y

Y

Y

Y

R

H

A

A

G

G

G

G

V

V

P

P

A

A

V

V

F

V

G

N

Q

Q

L

L

I

M

E

G

Q

Q

G

G

L

L

I

I

H

H

Q

E

D

D

A

A

R

I

A

T

V

V

S

N

G

G

Q

K

S

T

I

I

G

G

E

E

Q

N

Y

C

R

K

G

N

A

A

V

T

I

I

E

E

D

D

E

G

D

N

V

V

I

I

R

K

P

T

F

Y

A

D

R

Q

P

P

L

L

V

K

E

K

H

H

A

A

G

G

F

F

A

R

I

V

M

L

R

R

G

G

N

N

L

L

F

F

N

S

S

S

A

A

I

I

M

M

K

K

T

L

S

S

V

V

I

I

S

S

E

D

E

E

F

F

R

R

A

N

R

R

Y

Y

L

L

S

S

N

D

P

A

D

K

D

D

P

P

D

N

A

A

F

F

E

E

G

G

N

K

A

A

I

V

V

V

F

F

D

D

G

G

P

P

E

E

D

D

Y

Y

H

H

H

H

R

R

I

I

D

D

D

D

P

P

A

A

L

L

G

E

I

I

T

D

A

E

Y

H

S

T

I

V

L

L

F

F

M

M

R

R

G

G

A

A

G

G

P

P

I

I

G

G

Y

Y

P

P

G

G

S

A

A

A

E

E

V

V

V

V

N

N

M

M

R

R

A

A

P

P

N

D

Y

Y

L

L

I

L

K

K

Q

K

G

G

I

I

H

T

Q

S

L

L

P

P

C

C

I

I

G

G

D

D

G

G

R

R

Q

Q

S

S

G

G

T

T

S

S

G

G

S

S

P

P

S

S

I

I

L

L

N

N

A

A

S

S

P

P

E

E

A

A

A

A

A

A

G

G

G

G

G

G

L

L

A

A

L

I

L

L

K

K

T

T

G

G

D

D

R

R

V

V

R

R

F

I

D

D

L

L

R

G

K

R

S

G

R

T

V

A

D

D

V

I

L

L

V

I

S

S

A

D

S

E

E

E

V

L

V

A

E

E

R

R

R

R

R

K

A

A

L

L

E

E

A

A

A

V

G

G

G

G

Y

Y

A

K

Y

Y

P

P

E

E

S

S

Q

Q

T

T

P

P

W

W

Q

Q

E

E

I

I

Q

Q

R

R

A

A

V

V

V

I

G

G

Q

Q

M

M

D

E

T

T

G

G

A

A

V

V

L

L

E

E

P

N

A

A

V

V

K

K

Y

Y

Q

Q

R

D

I

I

A

A

Q

H

T

T

K

R

G

G

L

L

P

P

binding zinc ion: C53 (= C62), C121 (= C130)

Q9A9Z2 D-xylonate dehydratase; XyDHT; Gluconate dehydratase; EC 4.2.1.82; EC 4.2.1.39 from Caulobacter vibrioides (strain ATCC 19089 / CB15) (Caulobacter crescentus) (see 2 papers)
62% identity, 99% coverage: 6:600/600 of query aligns to 9:595/595 of Q9A9Z2

query
sites
Q9A9Z2

V

T

P

P

R

R

R

R

A

-

L

F

R

R

S

S

R

R

A

D

W

W

F

F

D

D

N

N

P

P

D

D

N

H

I

I

D

D

M

M

T

T

A

A

L

L

Y

Y

L

L

E

E

R

R

Y

F

L

M

N

N

F

Y

G

G

L

I

T

T

L

P

E

E

E

E

L

L

Q

R

S

S

G

G

K

K

P

P

I

I

I

I

G

G

I

I

A

A

Q

Q

T

T

G

G

S

S

D

D

L

I

S

S

P

P

C
|
C

N

N

R

R

H

I

H

H

L

L

V

D

L

L

A

V

E

Q

R

R

V

V

R

R

E

D

G

G

I

I

R

R

S

D

A

A

G

G

G

G

I

I

A

P

L

M

E

E

F

F

P

P

V

V

H

H

P

P

I

I

Q

F

E
|
E

T

N

G

C

K

R

R

R

P

P

T

T

A

A

G

A

L

L

D

D

R

R

N

N

L

L

S

S

Y

Y

L

L

G

G

L

L

V

V

E

E

L

T

L

L

Y

H

G

G

Y

Y

P

P

L

I

D

D

G

A

V

V

V

V

L

L

T

T

I

T

G

G

C
|
C

D
|
D

K

K

T

T

T

T

P

P

A

A

C

G

L

I

M

M

A

A

A

A

A

T

T

T

V

V

N

N

I

I

P

P

A

A

I

I

A

V

L

L

S

S

V

G

G

G

P

P

M

M

L

L

N

D

G

G

W

W

H

H

K

E

G

N

K

E

R

L

T

V

G

G

S

S

G

G

T

T

I

V

V

I

W

W

K

R

A

S

R

R

E

R

M

K

L

L

A

A

A

A

G

G

E

E

I

I

D

T

N

E

A

E

G

E

F

F

I

I

K

D

L

R

V

A

A

A

S

S

S

S

A

A

P

P

S

S

T

A

G

G

Y

H

C
|
C

N

N

T

T

M

M

G

G

T

T

A

A

T

S

T

T

M

M

N

N

S

A

L

V

T

A

E

E

A

A

L

L

G

G

M

L

S

S

L

L

P

T

G

G

S

C

A

A

A

A

I

I

P

P

A

A

P

P

Y

Y

R

R

D

E

R

R

Q

G

E

Q

N

M

A

A

Y

Y

R

K

T

T

G

G

L

Q

R

R

I

I

V

V

E

D

M

L

V

A

A

Y

E

D

D

D

L

V

K

K

P

P

S

L

D

D

I

I

L

L

T

T

R

K

E

Q

A

A

F

F

L

E

N

N

A

A

I

I

V

A

V

L

N

V

S

A

A

A

I

A

G

G

G

G

S

S

T

T

N

N

A

A

P

Q

I

P

H

H

L

I

N

V

A

A

L

M

A

A

R

R

H

H

M

A

D

G

V

V

D

E

L

I

T

T

L

A

E

D

D

D

W

W

E

R

T

-

A

A

G

A

K

Y

D

D

V

I

P

P

L

L

L

I

V

V

N

N

L

M

Q

Q

P

P

A

A

G

G

E

K

Y

Y

L

L

G

G

E

E

D

R

Y

F

Y

H

R

R

A

A

G

G

G

G

V

A

P

P

A

A

V

V

F

L

G

W

Q

E

L

L

I

L

E

Q

Q

Q

G

G

L

R

I

L

H

H

Q

G

D

D

A

V

R

L

A

T

V

V

S

T

G

G

Q

K

S

T

I

M

G

S

E

E

Q

N

Y

L

R

Q

G

G

A

R

V

E

I

T

E

S

D

D

E

R

D

E

V

V

I

I

R

F

P

P

F

Y

A

H

R

E

P

P

L

L

V

A

E

E

H

K

A

A

G

G

F

F

A

L

I

V

M

L

R

K

G

G

N

N

L

L

F

F

N

D

S

F

A

A

I

I

M

M

K

K

T

S

S

S

V

V

I

I

S

G

E

E

E

E

F

F

R

R

A

K

R

R

Y

Y

L

L

S

S

N

Q

P

P

D

G

D

Q

P

E

D

G

A

V

F

F

E

E

G

A

N

R

A

A

I

I

V

V

F

F

D

D

G

G

P

S

E

D

D

D

Y

Y

H

H

H

K

R

R

I

I

D

N

D

D

P

P

A

A

L

L

G

E

I

I

T

D

A

E

Y

R

S
x
C

I

I

L

L

F

V

M

I

R

R

G

G

A

A

G

G

P

P

I

I

G

G

Y

W

P

P

G

G

S

S

A

A

E
|
E

V

V

V

V

N

N

M

M

R

Q

A

P

P

P

N

D

Y

H

L

L

I

L

K

K

Q

K

G

G

I

I

H

M

Q

S

L

L

P

P

C

T

I

L

G

G

D

D

G

G

R

R

Q

Q

S

S

G

G

T

T

S

A

G

D

S

S

P

P

S

S

I

I

L

L

N

N

A

A

S

S

P

P

E

E

A

S

A

A

A

I

G

G

G

G

G

G

L

L

A

S

L

W

L

L

K

R

T

T

G

G

D

D

R

T

V

I

R

R

F

I

D

D

L

L

R

N

K

T

S

G

R

R

V

C

D

D

V

A

L

L

V

V

S

D

A

E

S

A

E

T

V

I

V

A

E

A

R

R

R

K

R

Q

A

-

L

-

E

-

A

-

A

D

G

G

G

I

Y

P

A

A

Y

V

P

P

E

A

S

T

Q

M

T

T

P

P

W

W

Q

Q

E

E

I

I

Q

Y

R

R

A

A

V

H

V

A

G

S

Q

Q

M

L

D

D

T

T

G

G

A

G

V

V

L

L

E

E

P

F

A

A

V

V

K

K

Y

Y

Q

Q

R

D

I

L

A

A

Q

A

T

K

K

-

G

-

L

L

P

P

R

R

D

H

N

N

H

H

C64 (= C62) binding ; mutation to S: Strong decrease in activity. Does not bind iron-sulfur cluster.
E96 (= E94) binding
C132 (= C130) binding ; mutation to S: Almost loss of activity. Does not bind iron-sulfur cluster.
D133 (= D131) binding
C205 (= C203) binding ; mutation to S: Strong decrease in activity. Does not bind iron-sulfur cluster.
C450 (≠ S449) mutation to S: Slight decrease in activity. Does not affect binding of iron-sulfur cluster.
E467 (= E466) binding

5oynA Crystal structure of d-xylonate dehydratase in holo-form (see paper)
62% identity, 99% coverage: 6:600/600 of query aligns to 3:589/589 of 5oynA

query
sites
5oynA

V

T

P

P

R

R

R

R

A

-

L

F

R

R

S

S

R

R

A

D

W

W

F

F

D

D

N

N

P

P

D

D

N

H

I

I

D

D

M

M

T

T

A

A

L

L

Y

Y

L

L

E

E

R

R

Y

F

L

M

N

N

F

Y

G

G

L

I

T

T

L

P

E

E

E

E

L

L

Q

R

S

S

G

G

K

K

P

P

I

I

I

I

G

G

I

I

A

A

Q

Q

T

T

G

G

S

S

D

D

L

I

S

S

P

P

C
|
C

N

N

R

R

H

I

H

H

L

L

V

D

L

L

A

V

E

Q

R

R

V

V

R

R

E

D

G

G

I

I

R

R

S

D

A

A

G

G

G

G

I

I

A

P

L

M

E

E

F

F

P

P

V

V

H

H

P

P

I

I

Q

F

E
|
E

T
x
N

G

C

K

R

R

R

P

P

T

T

A

A

G

A

L

L

D

D

R

R

N

N

L

L

S

S

Y

Y

L

L

G

G

L

L

V

V

E

E

L

T

L

L

Y

H

G

G

Y

Y

P

P

L

I

D

D

G

A

V

V

V

V

L

L

T

T

I

T

G

G

C
|
C

D
|
D

K
|
K

T

T

T

T

P

P

A

A

C

G

L

I

M

M

A

A

A

A

A

T

T

T

V

V

N

N

I

I

P

P

A

A

I

I

A

V

L

L

S

S

V

G

G

G

P

P

M

M

L

L

N

D

G

G

W

W

H

H

K

E

G

N

K

E

R

L

T

V

G

G

S

S

G

G

T

T

I

V

V

I

W

W

K

R

A

S

R

R

E

R

M

K

L

L

A

A

A

A

G

G

E

E

I

I

D

T

N

E

A

E

G

E

F

F

I

I

K

D

L

R

V

A

A

A

S

S

S

S

A

A

P

P

S

S

T

A

G

G

Y

H

C
|
C

N

N

T

T

M

M

G

G

T

T

A

A

T

S

T

T

M

M

N

N

S

A

L

V

T

A

E

E

A

A

L

L

G

G

M

L

S

S

L

L

P

T

G

G

S

C

A

A

A

A

I

I

P

P

A

A

P

P

Y

Y

R

R

D

E

R

R

Q

G

E

Q

N

M

A

A

Y

Y

R

K

T

T

G

G

L

Q

R

R

I

I

V

V

E

D

M

L

V

A

A

Y

E

D

D

D

L

V

K

K

P

P

S

L

D

D

I

I

L

L

T

T

R

K

E

Q

A

A

F

F

L

E

N

N

A

A

I

I

V

A

V

L

N

V

S

A

A

A

I

A

G

G

G

G

S

S

T

T

N

N

A

A

P

Q

I

P

H

H

L

I

N

V

A

A

L

M

A

A

R

R

H

H

M

A

D

G

V

V

D

E

L

I

T

T

L

A

E

D

D

D

W

W

E

R

T

-

A

A

G

A

K

Y

D

D

V

I

P

P

L

L

L

I

V

V

N

N

L

M

Q

Q

P

P

A

A

G

G

E

K

Y

Y

L

L

G

G

E

E

D

R

Y

F

Y

H

R

R

A

A

G

G

G

G

V

A

P

P

A

A

V

V

F

L

G

W

Q

E

L

L

I

L

E

Q

Q

Q

G

G

L

R

I

L

H

H

Q

G

D

D

A

V

R

L

A

T

V

V

S

T

G

G

Q

K

S

T

I

M

G

S

E

E

Q

N

Y

L

R

Q

G

G

A

R

V

E

I

T

E

S

D

D

E

R

D

E

V

V

I

I

R

F

P

P

F

Y

A

H

R

E

P

P

L

L

V

A

E

E

H

K

A

A

G

G

F

F

A

L

I

V

M

L

R

K

G

G

N

N

L

L

F

F

N

D

S

F

A

A

I

I

M

M

K

K

T

S

S

S

V

V

I

I

S

G

E

E

E

E

F

F

R

R

A

K

R

R

Y

Y

L

L

S

S

N

Q

P

P

D

G

D

Q

P

E

D

G

A

V

F

F

E

E

G

A

N

R

A

A

I

I

V

V

F

F

D

D

G

G

P

S

E

D

D

D

Y

Y

H

H

H

K

R

R

I

I

D

N

D

D

P

P

A

A

L

L

G

E

I

I

T

D

A

E

Y

R

S

C

I

I

L

L

F

V

M

I

R

R

G

G

A

A

G

G

P

P

I

I

G

G

Y

W

P

P

G

G

S

S

A

A

E
|
E

V

V

V

V

N

N

M

M

R

Q

A

P

P

P

N

D

Y

H

L

L

I

L

K

K

Q

K

G

G

I

I

H

M

Q

S

L

L

P

P

C

T

I

L

G

G

D

D

G

G

R

R

Q

Q

S

S

G

G

T

T

S

A

G

D

S

S

P

P

S

S

I

I

L

L

N

N

A

A

S

S

P

P

E

E

A

S

A

A

A

I

G

G

G

G

G

G

L

L

A

S

L

W

L

L

K

R

T

T

G

G

D

D

R

T

V

I

R

R

F

I

D

D

L

L

R

N

K

T

S

G

R

R

V

C

D

D

V

A

L

L

V

V

S

D

A

E

S

A

E

T

V

I

V

A

E

A

R

R

R

K

R

Q

A

-

L

-

E

-

A

-

A

D

G

G

G

I

Y

P

A

A

Y

V

P

P

E

A

S

T

Q

M

T

T

P

P

W

W

Q

Q

E

E

I

I

Q

Y

R

R

A

A

V

H

V

A

G

S

Q

Q

M

L

D

D

T

T

G

G

A

G

V

V

L

L

E

E

P

F

A

A

V

V

K

K

Y

Y

Q

Q

R

D

I

L

A

A

Q

A

T

K

K

-

G

-

L

L

P

P

R

R

D

H

N

N

H

H

binding fe2/s2 (inorganic) cluster: C58 (= C62), N91 (≠ T95), C126 (= C130), D127 (= D131), C199 (= C203)
binding magnesium ion: E90 (= E94), D127 (= D131), K128 (= K132), E461 (= E466)

B5ZZ34 L-arabinonate dehydratase; ArDHT; D-fuconate dehydratase; Galactonate dehydratase; L-arabonate dehydratase; EC 4.2.1.25; EC 4.2.1.67; EC 4.2.1.6 from Rhizobium leguminosarum bv. trifolii (strain WSM2304) (see 2 papers)
43% identity, 99% coverage: 9:600/600 of query aligns to 9:579/579 of B5ZZ34

query
sites
B5ZZ34

R

R

A

K

L

L

R

R

S

S

R

Q

A

E

W

W

F

Y

D

G

N

G

P

T

D

S

N

R

I

-

D

-

M

-

T

D

A

V

L

I

Y

Y

L

H

E

R

R

G

Y

W

L

L

N

K

F

N

G

Q

L

G

T

Y

L

P

E

H

E

D

L

L

Q

F

S

D

G

G

K

R

P

P

I

V

I

I

G

G

I

I

A

L

Q

N

T

T

G

W

S

S

D

D

L

M

S

T

P

P

C
|
C

N

N

R

G

H

H

H

L

L

R

V

E

L

L

A

A

E

E

R

K

V

V

R

K

E

A

G

G

I

V

R

W

S

E

A

A

G

G

G

G

I

F

A

P

L

L

E

E

F

V

P

P

V

V

H

F

P

S

I

A

Q

S

E
|
E

T

N

G

T

K

F

R

R

P

P

T

T

A

A

G

M

L

M

D

Y

R

R

N

N

L

L

S

A

Y

A

L

L

G

A

L

V

V

E

E

E

L

A

L

I

Y

R

G

G

Y

Q

P

P

L

M

D

D

G

G

V

C

V

V

L

L

T

L

I

V

G

G

C
|
C

D
|
D

K

K

T

T

T

T

P

P

A

S

C

L

L

L

M

M

A

G

A

A

A

A

T

S

V

C

N

D

I

L

P

P

A

S

I

I

A

V

L

V

S

T

V

G

G

G

P

P

M

M

L

L

N

N

G

G

W

Y

H

F

K

R

G

G

K

E

R

R

T

V

G

G

S

S

G

G

T

T

I

H

V

L

W

W

K

K

A

F

R

S

E

E

M

M

L

V

A

K

A

A

G

G

E

E

I

M

D

T

N

Q

A

A

G

E

F

F

I

L

K

E

L

A

V

E

A

A

S

S

S

M

A

S

P

R

S

S

T

S

G

G

Y

T

C
|
C

N

N

T

T

M

M

G

G

T

T

A

A

T

S

T

T

M

M

N

A

S

S

L

M

T

A

E

E

A

A

L

L

G

G

M

M

S

A

L

L

P

S

G

G

S

N

A

A

A

A

I

I

P

P

A

G

P

V

Y

D

R

S

D

R

R

R

Q

K

E

V

N

M

A

A

Y

Q

R

L

T

T

G

G

L

R

R

R

I

I

V

V

E

Q

M

M

V

V

A

K

E

D

D

D

L

L

K

K

P

P

S

S

D

E

I

I

L

M

T

T

R

K

E

Q

A

A

F

F

L

E

N

N

A

A

I

I

V

R

V

T

N

N

S

A

A

A

I

I

G

G

G

G

S

S

T

T

N

N

A

A

P

V

I

I

H

H

L

L

N

L

A

A

L

I

A

A

R

G

H

R

M

V

D

G

V

I

D

D

L

L

T

S

L

L

E

D

D

D

W

W

E

D

T

R

A

C

G

G

K

R

D

D

V

V

P

P

L

T

L

I

V

V

N

N

L

L

Q

M

P

P

A

S

G

G

E

K

Y

Y

L

L

G

M

E

E

D

E

Y

F

Y

F

R

Y

A

A

G

G

G

G

V

L

P

P

A

V

V

V

F

L

G

K

Q

R

L

L

I

G

E

E

Q

A

G

G

L

L

I

L

H

H

Q

K

D

D

A

A

R

L

A

T

V

V

S

S

G

G

Q

E

S

T

I

V

G

W

E

D

Q

E

Y

V

R

K

G

D

A

V

V

V

I

N

E

W

D

N

E

E

D

D

V

V

I

I

R

L

P

P

F

A

A

E

R

K

P

A

L

L

V

T

E

S

H

S

A

G

G

G

F

I

A

V

I

V

M

L

R

R

G

G

N

N

L

L

F

A

-

P

N

K

S

G

A

A

I

V

M

L

K

K

T

P

S

S

V

A

I

A

S

S

E

P

E

H

F

L

R

L

A

V

R

H

Y

-

L

-

S

-

N

-

P

-

D

-

D

-

P

-

D

-

A

-

F

-

E

K

G

G

N

R

A

A

I

V

V

V

F

F

D

E

G

D

P

I

E

D

D

D

Y

Y

H

K

H

A

R

K

I

I

D

N

D

D

P

D

A

N

L

L

G

D

I

I

T

D

A

E

Y

N

S
x
C

I

I

L

M

F

V

M

M

R

K

G

N

A
x
C

G

G

P

P

I

K

G

G

Y

Y

P

P

G

G

S

M

A

A

E
|
E

V

V

V

G

N

N

M

M

R

G

A

L

P

P

N

P

Y

K

L

V

I

L

K

K

Q

K

G

G

I

I

H

L

Q

D

L

M

P

V

C

R

I

I

G

S

D

D

G

A

R

R

Q

M

S
|
S

G

G

T

T

S

A

G

Y

S

G

P

T

S

V

I

V

L

L

N

H

A

T

S

S

P

P

E

E

A

A

A

A

A

V

G

G

G

G

G

P

L

L

A

A

L

V

L

V

K

K

T

N

G

G

D

D

R

M

V

I

R

E

F

L

D

D

L

V

R

P

K

N

S

R

R

R

V

L

D

H

V

L

L

D

V

I

S

S

A

D

S

E

E

E

V

L

V

A

E

-

R

-

R

R

R

R

A

L

L

A

E

E

A

W

A

Q

G

P

G

N

Y

H

A

D

Y

L

P

P

E

T

S

S

Q

G

T

Y

P

A

W

F

Q

L

E

H

I

Q

Q

Q

R

H

A

-

V

-

V

V

G

E

Q

G

M

A

D

D

T

T

G

G

A

A

V

D

L

L

E

D

P

-

A

-

V

-

K

-

Y

F

Q

L

R

K

I

G

A

C

Q

R

T

G

K

N

G

A

L

V

P

G

R

K

D

D

N

S

H

H

C59 (= C62) binding ; mutation to S: Loss of activity. Does not bind iron-sulfur cluster.
E91 (= E94) binding
C127 (= C130) binding ; mutation to S: Loss of activity. Does not bind iron-sulfur cluster.
D128 (= D131) binding
C200 (= C203) binding ; mutation to S: Almost loss of activity. Does not bind iron-sulfur cluster.
C436 (≠ S449) mutation to S: No change in activity. Does not affect binding of iron-sulfur cluster.
C443 (≠ A456) mutation to S: Slight decrease in activity. Does not affect binding of iron-sulfur cluster.
E453 (= E466) binding
S480 (= S493) mutation to A: Loss of activity.

5j85A Ser480ala mutant of l-arabinonate dehydratase (see paper)
43% identity, 99% coverage: 9:600/600 of query aligns to 6:576/576 of 5j85A

query
sites
5j85A

R

R

A

K

L

L

R

R

S

S

R

Q

A

E

W

W

F

Y

D

G

N

G

P

T

D

S

N

R

I

-

D

-

M

-

T

D

A

V

L

I

Y

Y

L

H

E

R

R

G

Y

W

L

L

N

K

F

N

G

Q

L

G

T

Y

L

P

E

H

E

D

L

L

Q

F

S

D

G

G

K

R

P

P

I

V

I

I

G

G

I

I

A

L

Q

N

T

T

G

W

S

S

D

D

L

M

S

T

P

P

C
|
C

N

N

R

G

H

H

H

L

L

R

V

E

L

L

A

A

E

E

R

K

V

V

R

K

E

A

G

G

I

V

R

W

S

E

A

A

G

G

G

G

I

F

A

P

L

L

E

E

F

V

P

P

V

V

H

F

P

S

I

A

Q

S

E
|
E

T
x
N

G

T

K

F

R

R

P

P

T

T

A

A

G

M

L

M

D

Y

R

R

N

N

L

L

S

A

Y

A

L

L

G

A

L

V

V

E

E

E

L

A

L

I

Y

R

G

G

Y

Q

P

P

L

M

D

D

G

G

V

C

V

V

L

L

T

L

I

V

G

G

C
|
C

D
|
D

K
|
K

T

T

T

T

P

P

A

S

C

L

L

L

M

M

A

G

A

A

A

A

T

S

V

C

N

D

I

L

P

P

A

S

I

I

A

V

L

V

S

T

V

G

G

G

P

P

M

M

L

L

N

N

G

G

W

Y

H

F

K

R

G

G

K

E

R

R

T

V

G

G

S

S

G

G

T

T

I

H

V

L

W

W

K

K

A

F

R

S

E

E

M

M

L

V

A

K

A

A

G

G

E

E

I

M

D

T

N

Q

A

A

G

E

F

F

I

L

K

E

L

A

V

E

A

A

S

S

S

M

A

S

P

R

S

S

T

S

G

G

Y
x
T

C
|
C

N

N

T

T

M

M

G

G

T
|
T

A

A

T

S

T

T

M

M

N

A

S

S

L

M

T

A

E

E

A

A

L

L

G

G

M

M

S

A

L

L

P

S

G

G

S

N

A

A

A

A

I

I

P

P

A

G

P

V

Y

D

R

S

D

R

R

R

Q

K

E

V

N

M

A

A

Y

Q

R

L

T

T

G

G

L

R

R

R

I

I

V

V

E

Q

M

M

V

V

A

K

E

D

D

D

L

L

K

K

P

P

S

S

D

E

I

I

L

M

T

T

R

K

E

Q

A

A

F

F

L

E

N

N

A

A

I

I

V

R

V

T

N

N

S

A

A

A

I

I

G

G

G

G

S

S

T

T

N

N

A

A

P

V

I

I

H

H

L

L

N

L

A

A

L

I

A

A

R

G

H

R

M

V

D

G

V

I

D

D

L

L

T

S

L

L

E

D

D

D

W

W

E

D

T

R

A

C

G

G

K

R

D

D

V

V

P

P

L

T

L

I

V

V

N

N

L

L

Q

M

P

P

A

S

G

G

E

K

Y

Y

L

L

G

M

E

E

D

E

Y

F

Y

F

R

Y

A

A

G

G

G

G

V

L

P

P

A

V

V

V

F

L

G

K

Q

R

L

L

I

G

E

E

Q

A

G

G

L

L

I

L

H

H

Q

K

D

D

A

A

R

L

A

T

V

V

S

S

G

G

Q

E

S

T

I

V

G

W

E

D

Q

E

Y

V

R

K

G

D

A

V

V

V

I

N

E

W

D

N

E

E

D

D

V

V

I

I

R

L

P

P

F

A

A

E

R

K

P

A

L

L

V

T

E

S

H

S

A

G

G

G

F

I

A

V

I

V

M

L

R

R

G

G

N

N

L

L

F

A

-

P

N

K

S

G

A

A

I

V

M

L

K

K

T

P

S

S

V

A

I

A

S

S

E

P

E

H

F

L

R

L

A

V

R

H

Y

-

L

-

S

-

N

-

P

-

D

-

D

-

P

-

D

-

A

-

F

-

E

K

G

G

N

R

A

A

I

V

V

V

F

F

D

E

G

D

P

I

E

D

D

D

Y

Y

H

K

H

A

R

K

I

I

D

N

D

D

P

D

A

N

L

L

G

D

I

I

T

D

A

E

Y

N

S

C

I

I

L

M

F

V

M

M

R

K

G

N

A

C

G

G

P

P

I

K

G

G

Y

Y

P

P

G

G

S

M

A

A

E
|
E

V

V

V

G

N

N

M

M

R

G

A

L

P

P

N

P

Y

K

L

V

I

L

K

K

Q

K

G

G

I

I

H

L

Q

D

L

M

P

V

C

R

I

I

G

S

D

D

G

A

R

R

Q

M

S

A

G

G

T

T

S

A

G

Y

S

G

P

T

S

V

I

V

L

L

N

H

A

T

S

S

P

P

E

E

A

A

A

A

A

V

G

G

G

G

G

P

L

L

A

A

L

V

L

V

K

K

T

N

G

G

D

D

R

M

V

I

R

E

F

L

D

D

L

V

R

P

K

N

S

R

R

R

V

L

D

H

V

L

L

D

V

I

S

S

A

D

S

E

E

E

V

L

V

A

E

-

R

-

R

R

R

R

A

L

L

A

E

E

A

W

A

Q

G

P

G

N

Y

H

A

D

Y

L

P

P

E

T

S

S

Q

G

T

Y

P

A

W

F

Q

L

E

H

I

Q

Q

Q

R

H

A

-

V

-

V

V

G

E

Q

G

M

A

D

D

T

T

G

G

A

A

V

D

L

L

E

D

P

-

A

-

V

-

K

-

Y

F

Q

L

R

K

I

G

A

C

Q

R

T

G

K

N

G

A

L

V

P

G

R

K

D

D

N

S

H

H

binding fe2/s2 (inorganic) cluster: C56 (= C62), N89 (≠ T95), C124 (= C130), D125 (= D131), T196 (≠ Y202), C197 (= C203)
binding magnesium ion: E88 (= E94), D125 (= D131), K126 (= K132), T202 (= T208), E450 (= E466)

8ej0A Crystal structure of fe-s cluster-dependent dehydratase from paralcaligenes ureilyticus in complex with mg
43% identity, 98% coverage: 12:600/600 of query aligns to 3:568/568 of 8ej0A

query
sites
8ej0A

R

R

S

S

R

Q

A

A

W

W

F

F

D

G

N

R

P

L

D

D

N

R

I

D

D

G

M

F

T

-

A

-

L

I

Y

Y

L

R

E

S

R

W

Y

M

L

K

N

N

F

R

G

G

L

I

T

P

L

H

E

D

E

Q

L

F

Q

D

S

-

G

G

K

R

P

P

I

V

I

I

G

G

I

I

A

C

Q

N

T

T

G

F

S

S

D

E

L

L

S

T

P

P

C
|
C

N

N

R

S

H

H

H

F

L

R

V

T

L

L

A

A

E

E

R

Q

V

V

R

K

E

I

G

G

I

V

R

W

S

E

A

S

G

G

G

G

I

F

A

P

L

L

E

E

F

F

P

P

V

V

H

M

P

S

I

L

Q

G

E
|
E

T

T

G

M

K

L

R

R

P

P

T

T

A

A

G

M

L

L

D

F

R

R

N

N

L

L

S

A

Y

S

L

M

G

D

L

V

V

E

E

E

L

S

L

I

Y

R

G

G

Y

N

P

P

L

L

D

D

G

G

V

V

V

V

L

L

T

L

I

M

G

G

C
|
C

D
|
D

K
|
K

T

T

T

T

P

P

A

S

C

L

L

M

M

M

A

G

A

A

A

A

T

S

V

C

N

D

I

L

P

P

A

T

I

I

A

G

L

V

S

S

V

G

G

G

P

P

M

M

L

L

N

S

G

G

W

K

H

F

K

R

G

G

K

R

R

E

T

L

G

G

S

S

G

G

T

T

I

D

V

V

W
|
W

K

K

A

M

R

S

E

E

M

E

L

V

A

R

A

A

G

G

E

Q

I

M

D

S

N

Q

A

E

G

E

F

F

I

F

K

E

L

A

V

E

A

S

S

C

S

M

A

H

P

R

S

S

T

H

G

G

Y

H

C
|
C

N

M

T

T

M

M

G

G

T
|
T

A

A

T

S

T

T

M

M

N

A

S

S

L

M

T

V

E

E

A

A

L

L

G

G

M

M

S

S

L

L

P

P

G

G

S

N

A

A

A

A

I

I

P

P

A

A

P

V

Y

D

R

A

D

R

R

R

Q

N

E

L

N

L

A

A

Y

R

R

A

T

S

G

G

L

R

R

R

I

I

V

V

E

Q

M

M

V

V

A

K

E

D

D

D

L

L

K

V

P

M

S

S

D

K

I

I

L

L

T

T

R

R

E

Q

A

A

F

F

L

E

N

N

A

A

I

I

V

R

V

V

N

N

S

A

A

A

I

I

G

G

G

G

S

S

T

T

N

N

A

A

P

V

I

I

H

H

L

L

N

L

A

A

L

I

A

A

R

G

H

R

M

I

D

G

V

V

D

D

L

L

T

T

L

L

E

A

D

D

W

W

E

D

T

A

A

L

G

G

K

H

D

K

V

L

P

P

L

C

L

L

V

V

N

D

L

L

Q

Q

P

P

A

S

G

G

E

T

Y

H

L

L

G

M

E

E

D

D

Y

F

Y

Y

R

Y

A

A

G

G

G

G

V

V

P

P

A

A

V

V

F

I

G

R

Q

E

L

L

I

G

E

D

Q

-

G

-

L

V

I

I

H

A

Q

R

D

D

A

A

R

L

A

T

V

V

S

N

G

G

Q

Q

S

T

I

L

G

W

E

D

Q

N

Y

C

R

K

G

D

A

A

V

P

I

N

E

W

D

N

E

R

D

E

V

V

I

I

R

H

P

A

F

F

A

N

R

E

P

P

L

F

V

K

E

T

H

E

A

A

G

G

F

I

A

A

I

V

M

L

R

R

G

G

N

N

L

L

F

C

-

P

N

D

S

G

A

A

I

V

M

I

K

K

T

P

S

S

V

A

I

A

S

T

E

P

E

A

F

L

R

L

A

K

R

H

Y

-

L

-

S

-

N

-

P

-

D

-

D

-

P

-

D

-

A

-

F

-

E

K

G

G

N

R

A

A

I

V

V

V

F

F

D

E

G

N

P

S

E

E

D

H

Y

M

H

H

H

E

R

R

I

M

D

D

D

D

P

E

A

N

L

L

G

D

I

V

T

D

A

E

Y

N

S

C

I

V

L

L

F

V

M

L

R

K

G

N

A

C

G

G

P

P

I

R

G

G

Y

Y

P

P

G

G

S

M

A

A

E
|
E

V

A

V

G

N

N

M

M

R

P

A

L

P

P

N

P

Y

K

L

I

I

L

K

R

Q

K

G

G

I

I

H

T

Q

D

L

M

P

V

C

R

I

V

G

S

D

D

G

A

R

R

Q

M

S
|
S

G
|
G

T

T

S

A

G

Y

S

G

P

T

S

V

I

V

L

L

N

H

A

V

S

A

P

P

E

E

A

A

A

A

A

A

G

G

G

G

G

P

L

L

A

A

L

L

L

V

K

Q

T

D

G

G

D

D

R

I

V

I

R

E

F

L

D

D

L

V

R

A

K

A

S

R

R

K

V

L

D

H

V

L

L

H

V

V

S

S

A

D

S

E

E

E

V

L

V

A

E

R

R

R

R

R

R

E

A

A

L

W

E

Q

A

A

A

P

G

-

G

-

Y

-

A

P

Y

A

P

P

E

M

S

A

Q

R

T

G

P

-

W

W

Q

V

E

K

I

L

Q

Y

R

V

A

E

V

H

V

V

G

Q

Q

Q

M

A

D

N

T

L

G

G

A

A

V

D

L

L

E

D

P

-

A

-

V

-

K

-

Y

F

Q

L

R

R

I

G

A

K

Q

S

T

G

K

A

G

G

L

I

P

P

R

K

D

D

N

N

H

H

binding bicarbonate ion: D119 (= D131), W161 (= W173), T196 (= T208), E442 (= E466), S469 (= S493), S469 (= S493), G470 (= G494)
binding fe2/s2 (inorganic) cluster: C50 (= C62), E82 (= E94), C118 (= C130), D119 (= D131), C191 (= C203)
binding magnesium ion: E82 (= E94), D119 (= D131), K120 (= K132), E442 (= E466)

8epzA Crystal structure of fe-s cluster-dependent dehydratase from paralcaligenes ureilyticus in complex with mn
43% identity, 98% coverage: 12:600/600 of query aligns to 4:569/569 of 8epzA

query
sites
8epzA

R

R

S

S

R

Q

A

A

W

W

F

F

D

G

N

R

P

L

D

D

N

R

I

D

D

G

M

F

T

-

A

-

L

I

Y

Y

L

R

E

S

R

W

Y

M

L

K

N

N

F

R

G

G

L

I

T

P

L

H

E

D

E

Q

L

F

Q

D

S

-

G

G

K

R

P

P

I

V

I

I

G

G

I

I

A

C

Q

N

T

T

G

F

S

S

D

E

L

L

S

T

P

P

C
|
C

N

N

R

S

H

H

H

F

L

R

V

T

L

L

A

A

E

E

R

Q

V

V

R

K

E

I

G

G

I

V

R

W

S

E

A

S

G

G

G

G

I

F

A

P

L

L

E

E

F

F

P

P

V

V

H

M

P

S

I

L

Q

G

E
|
E

T

T

G

M

K

L

R

R

P

P

T

T

A

A

G

M

L

L

D

F

R

R

N

N

L

L

S

A

Y

S

L

M

G

D

L

V

V

E

E

E

L

S

L

I

Y

R

G

G

Y

N

P

P

L

L

D

D

G

G

V

V

V

V

L

L

T

L

I

M

G

G

C
|
C

D
|
D

K
|
K

T

T

T

T

P

P

A

S

C

L

L

M

M

M

A

G

A

A

A

A

T

S

V

C

N

D

I

L

P

P

A

T

I

I

A

G

L

V

S

S

V

G

G

G

P

P

M

M

L

L

N

S

G

G

W

K

H

F

K

R

G

G

K

R

R

E

T

L

G

G

S

S

G

G

T

T

I

D

V

V

W

W

K

K

A

M

R

S

E

E

M

E

L

V

A

R

A

A

G

G

E

Q

I

M

D

S

N

Q

A

E

G

E

F

F

I

F

K

E

L

A

V

E

A

S

S

C

S

M

A

H

P

R

S

S

T

H

G

G

Y

H

C
|
C

N

M

T

T

M

M

G

G

T

T

A

A

T

S

T

T

M

M

N

A

S

S

L

M

T

V

E

E

A

A

L

L

G

G

M

M

S

S

L

L

P

P

G

G

S

N

A

A

A

A

I

I

P

P

A

A

P

V

Y

D

R

A

D

R

R

R

Q

N

E

L

N

L

A

A

Y

R

R

A

T

S

G

G

L

R

R

R

I

I

V

V

E

Q

M

M

V

V

A

K

E

D

D

D

L

L

K

V

P

M

S

S

D

K

I

I

L

L

T

T

R

R

E

Q

A

A

F

F

L

E

N

N

A

A

I

I

V

R

V

V

N

N

S

A

A

A

I

I

G

G

G

G

S

S

T

T

N

N

A

A

P

V

I

I

H

H

L

L

N

L

A

A

L

I

A

A

R

G

H

R

M

I

D

G

V

V

D

D

L

L

T

T

L

L

E

A

D

D

W

W

E

D

T

A

A

L

G

G

K

H

D

K

V

L

P

P

L

C

L

L

V

V

N

D

L

L

Q

Q

P

P

A

S

G

G

E

T

Y

H

L

L

G

M

E

E

D

D

Y

F

Y

Y

R

Y

A

A

G

G

G

G

V

V

P

P

A

A

V

V

F

I

G

R

Q

E

L

L

I

G

E

D

Q

-

G

-

L

V

I

I

H

A

Q

R

D

D

A

A

R

L

A

T

V

V

S

N

G

G

Q

Q

S

T

I

L

G

W

E

D

Q

N

Y

C

R

K

G

D

A

A

V

P

I

N

E

W

D

N

E

R

D

E

V

V

I

I

R

H

P

A

F

F

A

N

R

E

P

P

L

F

V

K

E

T

H

E

A

A

G

G

F

I

A

A

I

V

M

L

R

R

G

G

N

N

L

L

F

C

-

P

N

D

S

G

A

A

I

V

M

I

K

K

T

P

S

S

V

A

I

A

S

T

E

P

E

A

F

L

R

L

A

K

R

H

Y

-

L

-

S

-

N

-

P

-

D

-

D

-

P

-

D

-

A

-

F

-

E

K

G

G

N

R

A

A

I

V

V

V

F

F

D

E

G

N

P

S

E

E

D

H

Y

M

H

H

H

E

R

R

I

M

D

D

D

D

P

E

A

N

L

L

G

D

I

V

T

D

A

E

Y

N

S

C

I

V

L

L

F

V

M

L

R

K

G

N

A

C

G

G

P

P

I

R

G

G

Y

Y

P

P

G

G

S

M

A

A

E
|
E

V

A

V

G

N

N

M

M

R

P

A

L

P

P

N

P

Y

K

L

I

I

L

K

R

Q

K

G

G

I

I

H

T

Q

D

L

M

P

V

C

R

I

V

G

S

D

D

G

A

R

R

Q

M

S

S

G

G

T

T

S

A

G

Y

S

G

P

T

S

V

I

V

L

L

N

H

A

V

S

A

P

P

E

E

A

A

A

A

A

A

G

G

G

G

G

P

L

L

A

A

L

L

L

V

K

Q

T

D

G

G

D

D

R

I

V

I

R

E

F

L

D

D

L

V

R

A

K

A

S

R

R

K

V

L

D

H

V

L

L

H

V

V

S

S

A

D

S

E

E

E

V

L

V

A

E

R

R

R

R

R

R

E

A

A

L

W

E

Q

A

A

A

P

G

-

G

-

Y

-

A

P

Y

A

P

P

E

M

S

A

Q

R

T

G

P

-

W

W

Q

V

E

K

I

L

Q

Y

R

V

A

E

V

H

V

V

G

Q

Q

Q

M

A

D

N

T

L

G

G

A

A

V

D

L

L

E

D

P

-

A

-

V

-

K

-

Y

F

Q

L

R

R

I

G

A

K

Q

S

T

G

K

A

G

G

L

I

P

P

R

K

D

D

N

N

H

H

binding fe2/s2 (inorganic) cluster: C51 (= C62), C119 (= C130), D120 (= D131), C192 (= C203)
binding manganese (ii) ion: E83 (= E94), D120 (= D131), K121 (= K132), E443 (= E466)

Q1JUQ1 L-arabonate dehydratase; L-arabinonate dehydratase; EC 4.2.1.25 from Azospirillum brasilense (see paper)
46% identity, 89% coverage: 11:544/600 of query aligns to 8:529/583 of Q1JUQ1

query
sites
Q1JUQ1

L

L

R

R

S

S

R

T

A

Q

W

W

F

F

D

G

N

T

P

N

D

D

N

K

I

N

D

G

M

F

T

-

A

-

L

M

Y

Y

L

R

E

S

R

W

Y

M

L

K

N

N

F

Q

G

G

L

I

T

P

L

D

E

H

E

E

L

F

Q

D

S

-

G

G

K

R

P

P

I

I

I

I

G

G

I

I

A
x
C

Q

N

T

T

G

W

S

S

D

E

L

L

S

T

P

P

C
|
C

N

N

R

A

H

H

H

F

L

R

V

K

L

L

A

A

E

E

R

H

V

V

R

K

E

R

G

G

I

I

R

S

S

E

A

A

G

G

G

G

I

F

A

P

L

V

E

E

F

F

P

P

V

V

H

F

P

S

I

N

Q

G

E

E

T

S

G

N

K

L

R

R

P

P

T

S

A

A

G

M

L

L

D

T

R

R

N

N

L

L

S

A

Y

S

L

M

G

D

L

V

V

E

E

E

L

A

L

I

Y

R

G

G

Y

N

P

P

L

I

D

D

G

A

V

V

V

V

L

L

T

L

I

A

G

G

C
|
C

D

D

K

K

T

T

T

T

P

P

A

A

C

L

L

L

M

M

A

G

A

A

A

A

T

S

V

C

N

D

I

V

P

P

A

A

I

I

A

V

L

V

S

S

V

G

G

G

P

P

M

M

L

L

N

N

G

G

W

K

H

L

K

E

G

G

K

K

R

N

T

I

G

G

S

S

G

G

T

T

I

A

V

V

W

W

K

Q

A

L

R

H

E

E

M

A

L

L

A

K

A

A

G

G

E

E

I

I

D

D

N

V

A

H

G

H

F

F

I

L

K

S

L

A

V

E

A

A

S

G

S

M

A

S

P

R

S

S

T

A

G

G

Y

T

C
|
C

N

N

T

T

M

M

G

G

T

T

A

A

T

S

T

T

M

M

N

A

S

C

L

M

T

A

E

E

A

A

L

L

G

G

M

V

S

A

L

L

P

P

G

H

S

N

A

A

A

A

I

I

P

P

A

A

P

V

Y

D

R

S

D

R

R

R

Q

Y

E

V

N

L

A

A

Y

H

R

M

T

S

G

G

L

I

R

R

I

I

V

V

E

E

M

M

V

A

A

L

E

E

D

G

L

L

K

V

P

L

S

S

D

K

I

I

L

L

T

T

R

R

E

A

A

A

F

F

L

E

N

N

A

A

I

I

V

R

V

A

N

N

S

A

A

A

I

I

G

G

G

G

S

S

T

T

N

N

A

A

P

V

I

I

H

H

L

L

N

K

A

A

L

I

A

A

R

G

H

R

M

I

D

G

V

V

D

P

L

L

T

E

L

L

E

E

D

D

W

W

E

M

T

R

A

I

G

G

K

R

D

D

V

T

P

P

L

T

L

I

V

V

N

D

L

L

Q

M

P

P

A

S

G

G

E

R

Y

F

L

P

G

M

E

E

D

E

Y

F

Y

Y

R

Y

A

A

G

G

G

G

V

L

P

P

A

A

V

V

F

L

G

R

Q

R

L

L

I

G

E

E

Q

G

G

G

L

L

I

L

-

P

H

N

Q

P

D

D

A

A

R

L

A

T

V

V

S

N

G

G

Q

K

S

S

I

L

G

W

E

D

Q

N

Y

V

R

R

G

E

A

A

V

P

I

N

E

Y

D

D

E

E

D

E

V

V

I

I

R

R

P

P

F

L

A

D

R

R

P

P

L

L

V

I

E

A

H

D

A

G

G

G

F

I

A

R

I

I

M

L

R

R

G

G

N

N

L

L

F

A

-

P

N

R

S

G

A

A

I

V

M

L

K

K

T

P

S

S

V

A

I

A

S

S

E

P

E

E

F

L

R

L

A

K

R

H

Y

-

L

-

S

-

N

-

P

-

D

-

D

-

P

-

D

-

A

-

F

-

E

R

G

G

N

R

A

A

I

V

V

V

F

F

D

E

G

N

P

L

E

D

D

H

Y

Y

H

K

H

A

R

T

I

I

D

N

D

D

P

E

A

A

L

L

G

D

I

I

T

D

A

A

Y

S

S

S

I

V

L

M

F

V

M

L

R

K

G

N

A
x
C

G

G

P

P

I

R

G

G

Y

Y

P

P

G

G

S

M

A

A

E

E

V

V

V

G

N

N

M

M

R

G

A

L

P

P

N

P

Y

K

L

L

I

L

K

R

Q

Q

G

G

I

V

H

K

Q

D

L

M

P

V

C

R

I

I

G

S

D

D

G

A

R

R

Q

M

S

S

G

G

T

T

S

A

G

Y

S

G

P

T

S

V

I

V

L

L

N

H

A

V

S

A

P

P

E

E

A

A

A

A

A

A

G

G

G

G

G

P

L

L

A

A

L

A

L

V

K

R

T

N

G

G

D

D

R

W

V

I

R

E

F

L

D

D

L

C

R

E

K

A

S

G

R

T

V

L

D

H

V

L

L

D

V

I

S

T

A

D

S

D

E

E

V

L

V

H

E

R

R

R

C47 (≠ A53) mutation to A: 2-fold reduction in activity and still binds a 4Fe-4S cluster.
C56 (= C62) mutation to A: Loss of activity but seems to be able to bind a Fe-S cluster.
C124 (= C130) binding ; mutation to A: Loss of activity and no 4Fe-4S cluster binding.
C197 (= C203) binding ; mutation to A: Loss of activity and no 4Fe-4S cluster binding.
C441 (≠ A456) mutation to A: Increase in activity and still binds a 4Fe-4S cluster.

6ovtA Crystal structure of ilvd from mycobacterium tuberculosis (see paper)
35% identity, 85% coverage: 40:546/600 of query aligns to 29:531/562 of 6ovtA

query
sites
6ovtA

L

M

E

D

E

D

L