SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing CCNA_01496 FitnessBrowser__Caulo:CCNA_01496 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

3e0iA Cu2+ substituted aquifex aeolicus kdo8ps in complex with pep (see paper)
51% identity, 87% coverage: 29:278/287 of query aligns to 1:250/263 of 3e0iA

query
sites
3e0iA

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binding copper (ii) ion: C10 (= C38), H184 (= H212), E221 (= E249)
binding phosphoenolpyruvate: K40 (= K67), K45 (= K72), Q98 (= Q125), P100 (= P127), A101 (= A128), K123 (= K150), R153 (= R180), H184 (= H212)
binding phosphate ion: R48 (= R75), S49 (≠ T76), K195 (≠ S223), S196 (= S224)

2a21A Aquifex aeolicus kdo8ps in complex with pep, po4, and zn2+ (see paper)
51% identity, 87% coverage: 29:278/287 of query aligns to 1:250/263 of 2a21A

query
sites
2a21A

E

E

K

K

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binding phosphoenolpyruvate: K40 (= K67), K45 (= K72), Q98 (= Q125), P100 (= P127), A101 (= A128), K123 (= K150), R153 (= R180), H184 (= H212)
binding phosphate ion: R48 (= R75), S49 (≠ T76), S196 (= S224)
binding zinc ion: C10 (= C38), H184 (= H212), E221 (= E249), D232 (= D260)

1jcyA Aquifex aeolicus kdo8p synthase in complex with r5p, pep and cadmium (see paper)
51% identity, 87% coverage: 29:278/287 of query aligns to 1:250/263 of 1jcyA

query
sites
1jcyA

E

E

K

K

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binding phosphoenolpyruvate: K40 (= K67), K45 (= K72), Q98 (= Q125), P100 (= P127), A101 (= A128), K123 (= K150), R153 (= R180), H184 (= H212)
binding ribose-5-phosphate: N47 (= N74), R48 (= R75), S49 (≠ T76), H184 (= H212), Q187 (= Q215), S196 (= S224)

1fwwA Aquifex aeolicus kdo8p synthase in complex with pep, a5p and cadmium (see paper)
51% identity, 87% coverage: 29:278/287 of query aligns to 1:250/263 of 1fwwA

query
sites
1fwwA

E

E

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binding arabinose-5-phosphate: N47 (= N74), R48 (= R75), S49 (≠ T76), H184 (= H212), S196 (= S224), D232 (= D260)
binding phosphoenolpyruvate: K40 (= K67), K45 (= K72), Q98 (= Q125), P100 (= P127), A101 (= A128), K123 (= K150), R153 (= R180), H184 (= H212)

1fwtA Aquifex aeolicus kdo8p synthase in complex with pep, e4p and cadmium (see paper)
51% identity, 87% coverage: 29:278/287 of query aligns to 1:250/263 of 1fwtA

query
sites
1fwtA

E

E

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I

-

G

W

C

A

P

K

V

V

V

I

F

Y

D

D

A

A

T

T

H
|
H

S

S

V

V

Q

Q

Q

L

P

P

G

G

G

G

Q

L

G

G

T

D

S

K

S
|
S

G

G

G

G

Q

M

R

R

E

E

F

F

V

I

P

F

T

P

L

L

A

I

R

R

A

A

A

A

V

V

A

A

V

V

G

G

V

C

A

D

C

G

V

V

F

F

I

M

E

E

T

T

H

H

P

P

D

E

P

P

D

E

N

K

A

A

P

L

S

S

D

D

G

A

P

S

N

T

M

Q

V

L

P

P

V

L

K

S

E

Q

F

L

E

E

A

G

L

I

V

I

A

E

N

A

L

I

L

L

binding erythose-4-phosphate: N47 (= N74), R48 (= R75), S49 (≠ T76), S196 (= S224)
binding phosphoenolpyruvate: K40 (= K67), K45 (= K72), Q98 (= Q125), P100 (= P127), A101 (= A128), K123 (= K150), R153 (= R180), H184 (= H212)

2nx3D Structural and mechanistic changes along an engineered path from metallo to non-metallo kdo8p synthase (see paper)
50% identity, 87% coverage: 30:278/287 of query aligns to 1:249/262 of 2nx3D

query
sites
2nx3D

K

K

L

F

S

L

I

V

I

I

A

A

G

G

P

P

C
x
N

Q

A

M

I

E

E

S

S

R

E

Q

E

H

L

A

L

L

L

E

K

T

V

A

G

H

E

A

E

L

I

K

K

E

R

M

L

A

S

D

E

R

K

L

F

G

K

-

E

V

V

G

E

L

F

I

V

Y

F

K

K

T

S

S

S

Y

F

D

D

K
|
K

A

A

N
|
N

R
|
R

T
x
S

S

S

A

I

N

H

A

S

Q

F

R

R

G

G

I

H

G

G

L

L

K

E

D

Y

S

G

L

V

A

K

I

A

F

L

Q

R

E

K

I

V

R

K

E

E

V

E

T

F

G

G

L

L

P

K

T

I

L

T

T

T

D

D

V

I

H

H

E

E

T

S

S

W

H

Q

C

A

S

E

I

P

V

V

A

A

E

E

A

V

V

A

D

D

V

I

I

I

Q
|
Q

I

I

P
|
P

A
|
A

F

F

L

L

C

C

R

R

Q

Q

T

T

D

D

L

L

L

L

V

L

A

A

A

A

A

A

S

K

T

T

G

G

R

R

A

A

I

V

N

N

I

V

K
|
K

K

K

G

G

Q

Q

F

F

L

L

A

A

P

P

W

W

D

D

M

T

K

K

N

N

V

V

I

V

A

E

K

K

V

L

T

K

G

F

A

G

G

G

N

A

P

K

N

E

V

I

M

Y

A

L

C

T

E

E

R
|
R

G

G

A

T

S

T

F

F

G

G

Y

Y

N

N

T

N

L

L

V

V

S

V

D

D

M

F

R

R

A

S

L

L

P

P

I

I

M

M

K

K

E

Q

I

-

G

W

C

A

P

K

V

V

V

I

F

Y

D

D

A

A

T

T

H
|
H

S

S

V

V

Q

Q

Q

L

P

P

G

G

G

G

Q

L

G

G

T

D

S
x
K

S
|
S

G

G

G

G

Q

M

R

R

E

E

F

F

V

I

P

F

T

P

L

L

A

I

R

R

A

A

A

A

V

V

A

A

V

V

G

G

V

C

A

D

C

G

V

V

F

F

I

M

E

E

T

T

H

H

P

P

D

E

P

P

D

E

N

K

A

A

P

L

S

S

D
|
D

G

A

P

P

N

T

M

A

V

L

P

P

V

L

K

S

E

Q

F

L

E

E

A

G

L

I

V

I

A

E

N

A

L

I

L

L

binding (2r,4r,5r,6r,7r)-2,4,5,6,7-pentahydroxy-2,8-bis(phosphonooxy)octanoic acid: N9 (≠ C38), K44 (= K72), N46 (= N74), R47 (= R75), S48 (≠ T76), Q97 (= Q125), P99 (= P127), A100 (= A128), K122 (= K150), R152 (= R180), H183 (= H212), K194 (≠ S223), S195 (= S224), D231 (= D260)

2nx1A Structural and mechanistic changes along an engineered path from metallo to non-metallo kdo8p synthase (see paper)
50% identity, 87% coverage: 29:278/287 of query aligns to 1:250/263 of 2nx1A

query
sites
2nx1A

E

E

K

K

L

F

S

L

I

V

I

I

A

A

G

G

P

P

C

N

Q

A

M

I

E

E

S

S

R

E

Q

E

H

L

A

L

L

L

E

K

T

V

A

G

H

E

A

E

L

I

K

K

E

R

M

L

A

S

D

E

R

K

L

F

G

K

-

E

V

V

G

E

L

F

I

V

Y

F

K
|
K

T

S

S

S

Y

F

D

D

K
|
K

A

A

N
|
N

R
|
R

T
x
S

S

S

A

I

N

H

A

S

Q

F

R

R

G

G

I

H

G

G

L

L

K

E

D

Y

S

G

L

V

A

K

I

A

F

L

Q

R

E

K

I

V

R

K

E

E

V

E

T

F

G

G

L

L

P

K

T

I

L

T

T

T

D

D

V

I

H

H

E

E

T

S

S

W

H

Q

C

A

S

E

I

P

V

V

A

A

E

E

A

V

V

A

D

D

V

I

I

I

Q
|
Q

I

I

P
|
P

A
|
A

F

F

L

L

C

C

R

R

Q

Q

T

T

D

D

L

L

L

L

V

L

A

A

A

A

A

A

S

K

T

T

G

G

R

R

A

A

I

V

N

N

I

V

K
|
K

K

K

G

G

Q

Q

F

F

L

L

A

A

P

P

W

W

D

D

M

T

K

K

N

N

V

V

I

V

A

E

K

K

V

L

T

K

G

F

A

G

G

G

N

A

P

K

N

E

V

I

M

Y

A

L

C

T

E

E

R
|
R

G

G

A

T

S

T

F

F

G

G

Y

Y

N

N

T

N

L

L

V

V

S

V

D

D

M

F

R

R

A

S

L

L

P

P

I

I

M

M

K

K

E

Q

I

-

G

W

C

A

P

K

V

V

V

I

F

Y

D

D

A

A

T

T

H
|
H

S

S

V

V

Q

Q

Q

L

P

P

G

G

G

G

Q

L

G

G

T

D

S

K

S
|
S

G

G

G

G

Q

M

R

R

E

E

F

F

V

I

P

F

T

P

L

L

A

I

R

R

A

A

A

A

V

V

A

A

V

V

G

G

V

C

A

D

C

G

V

V

F

F

I

M

E

E

T

T

H

H

P

P

D

E

P

P

D

E

N

K

A

A

P

L

S

S

D
|
D

G

A

P

S

N

T

M

Q

V

L

P

P

V

L

K

S

E

Q

F

L

E

E

A

G

L

I

V

I

A

E

N

A

L

I

L

L

binding phosphoenolpyruvate: K40 (= K67), Q98 (= Q125), P100 (= P127), A101 (= A128), K123 (= K150), R153 (= R180), H184 (= H212)
binding 5-O-phosphono-beta-D-ribofuranose: K45 (= K72), N47 (= N74), R48 (= R75), S49 (≠ T76), S196 (= S224), D232 (= D260)

3e12A Cu2+ substituted aquifex aeolicus kdo8ps in complex with kdo8p (see paper)
50% identity, 87% coverage: 29:278/287 of query aligns to 1:245/258 of 3e12A

query
sites
3e12A

E

E

K

K

L

F

S

L

I

V

I

I

A

A

G

G

P

P

C
|
C

Q

A

M

I

E

E

S

S

R

E

Q

E

H

L

A

L

L

L

E

K

T

V

A

G

H

E

A

E

L

I

K

K

E

R

M

L

A

S

D

E

R

K

L

F

G

K

-

E

V

V

G

E

L

F

I

V

Y

F

K
|
K

T

S

S

S

Y

F

D

D

K
|
K

A

A

N
|
N

R
|
R

T
x
S

S

S

A

I

N

H

A

S

Q

F

R

R

G

G

I

H

G

G

L

L

K

E

D

Y

S

G

L

V

A

K

I

A

F

L

Q

R

E

K

I

V

R

K

E

E

V

E

T

F

G

G

L

L

P

K

T

I

L

T

T

T

D

D

V

I

H

H

E

E

T

S

S

W

H

Q

C

A

S

E

I

P

V

V

A

A

E

E

A

V

V

A

D

D

V

I

I

I

Q
|
Q

I

I

P
|
P

A
|
A

F

F

L

L

C

C

R

R

Q

Q

T

T

D

D

L

L

L

L

V

L

A

A

A

A

A

A

S

K

T

T

G

G

R

R

A

A

I

V

N

N

I

V

K
|
K

K

K

G

G

Q

Q

F

F

L

L

A

A

P

P

W

W

D

D

M

T

K

K

N

N

V

V

I

V

A

E

K

K

V

L

T

K

G

F

A

G

G

G

N

A

P

K

N

E

V

I

M

Y

A

L

C

T

E

E

R
|
R

G

G

A

T

S

T

F

F

G

G

Y

Y

N

N

T

N

L

L

V

V

S

V

D

D

M

F

R

R

A

S

L

L

P

P

I

I

M

M

K

K

E

Q

I

-

G

W

C

A

P

K

V

V

V

I

F

Y

D

D

A

A

T

T

H
|
H

S

S

V

V

Q

Q

Q

L

P

P

G

G

G

G

Q

-

G

-

T

-

S

-

S

-

G

G

G

G

Q

M

R

R

E

E

F

F

V

I

P

F

T

P

L

L

A

I

R

R

A

A

A

A

V

V

A

A

V

V

G

G

V

C

A

D

C

G

V

V

F

F

I

M

E
|
E

T

T

H

H

P

P

D

E

P

P

D

E

N

K

A

A

P

L

S

S

D
|
D

G

A

P

S

N

T

M

Q

V

L

P

P

V

L

K

S

E

Q

F

L

E

E

A

G

L

I

V

I

A

E

N

A

L

I

L

L

binding copper (ii) ion: C10 (= C38), H184 (= H212), E216 (= E249), D227 (= D260)
binding 3-deoxy-8-O-phosphono-D-manno-oct-2-ulosonic acid: K40 (= K67), K45 (= K72), N47 (= N74), R48 (= R75), S49 (≠ T76), Q98 (= Q125), K123 (= K150), H184 (= H212), D227 (= D260)
binding phosphate ion: P100 (= P127), A101 (= A128), K123 (= K150), R153 (= R180)

1pe1A Aquifex aeolicus kdo8ps in complex with cadmium and 2-pga (see paper)
49% identity, 87% coverage: 29:278/287 of query aligns to 2:245/258 of 1pe1A

query
sites
1pe1A

E

E

K

K

L

F

S

L

I

V

I

I

A

A

G

G

P

P

C

C

Q

A

M

I

E

E

S

S

R

E

Q

E

H

L

A

L

L

L

E

K

T

V

A

G

H

E

A

E

L

I

K

K

E

R

M

L

A

S

D

E

R

K

L

F

G

K

-

E

V

V

G

E

L

F

I

V

Y

F

K
|
K

T

S

S

S

Y

F

D

D

K

K

A

A

N

N

R

R

T

S

S

S

A

I

N

H

A

S

Q

F

R

R

G

G

I

H

G

G

L

L

K

E

D

Y

S

G

L

V

A

K

I

A

F

L

Q

R

E

K

I

V

R

K

E

E

V

E

T

F

G

G

L

L

P

K

T

I

L

T

T

T

D
|
D

V

I

H

H

E

E

T

S

S

W

H

Q

C

A

S

E

I

P

V

V

A

A

E

E

A

V

V

A

D

D

V

I

I

I

Q
|
Q

I

I

P
|
P

A
|
A

F

F

L

L

C

C

R

R

Q

Q

T

T

D

D

L

L

L

L

V

L

A

A

A

A

A

A

S

K

T

T

G

G

R

R

A

A

I

V

N

N

I

V

K
|
K

K

K

G

G

Q

Q

F

F

L

L

A

A

P

P

W

W

D

D

M

T

K

K

N

N

V

V

I

V

A

E

K

K

V

L

T

K

G

F

A

G

G

G

N

A

P

K

N

E

V

I

M

Y

A

L

C

T

E

E

R
|
R

G

G

A

T

S

T

F

F

G

G

Y

Y

N

N

T

N

L

L

V

V

S

V

D

D

M

F

R

R

A

S

L

L

P

P

I

I

M

M

K

K

E

Q

I

-

G

W

C

A

P

K

V

V

V

I

F

Y

D

D

A

A

T

T

H
|
H

S

S

V

V

Q

Q

Q

L

P

P

G

G

G

-

Q

-

G

-

T

-

S

-

S

-

G

G

G

G

Q

M

R

R

E

E

F

F

V

I

P

F

T

P

L

L

A

I

R

R

A

A

A

A

V

V

A

A

V

V

G

G

V

C

A

D

C

G

V

V

F

F

I

M

E

E

T

T

H

H

P

P

D

E

P

P

D

E

N

K

A

A

P

L

S

S

D

D

G

A

P

S

N

T

M

Q

V

L

P

P

V

L

K

S

E

Q

F

L

E

E

A

G

L

I

V

I

A

E

N

A

L

I

L

L

binding 2-phosphoglyceric acid: K41 (= K67), D81 (= D107), Q99 (= Q125), P101 (= P127), A102 (= A128), K124 (= K150), R154 (= R180), H185 (= H212)

1pcwA Aquifex aeolicus kdo8ps in complex with cadmium and app, a bisubstrate inhibitor (see paper)
49% identity, 87% coverage: 29:278/287 of query aligns to 1:244/257 of 1pcwA

query
sites
1pcwA

E

E

K

K

L

F

S

L

I

V

I

I

A

A

G

G

P

P

C

C

Q

A

M

I

E

E

S

S

R

E

Q

E

H

L

A

L

L

L

E

K

T

V

A

G

H

E

A

E

L

I

K

K

E

R

M

L

A

S

D

E

R

K

L

F

G

K

-

E

V

V

G

E

L

F

I

V

Y

F

K

K

T

S

S

S

Y

F

D

D

K
|
K

A

A

N
|
N

R
|
R

T
x
S

S

S

A

I

N

H

A

S

Q

F

R

R

G

G

I

H

G

G

L

L

K

E

D

Y

S

G

L

V

A

K

I

A

F

L

Q

R

E

K

I

V

R

K

E

E

V

E

T

F

G

G

L

L

P

K

T

I

L

T

T

T

D

D

V

I

H

H

E

E

T

S

S

W

H

Q

C

A

S

E

I

P

V

V

A

A

E

E

A

V

V

A

D

D

V

I

I

I

Q

Q

I

I

P
|
P

A
|
A

F

F

L

L

C

C

R

R

Q

Q

T

T

D

D

L

L

L

L

V

L

A

A

A

A

A

A

S

K

T

T

G

G

R

R

A

A

I

V

N

N

I

V

K
|
K

K

K

G

G

Q

Q

F

F

L

L

A

A

P

P

W

W

D

D

M

T

K

K

N

N

V

V

I

V

A

E

K

K

V

L

T

K

G

F

A

G

G

G

N

A

P

K

N

E

V

I

M

Y

A

L

C

T

E

E

R
|
R

G

G

A

T

S

T

F

F

G

G

Y

Y

N

N

T

N

L

L

V

V

S

V

D

D

M

F

R

R

A

S

L

L

P

P

I

I

M

M

K

K

E

Q

I

-

G

W

C

A

P

K

V

V

V

I

F

Y

D

D

A

A

T

T

H
|
H

S

S

V

V

Q
|
Q

Q

L

P

P

G

G

G

-

Q

-

G

-

T

-

S

-

S

-

G

G

G

G

Q

M

R

R

E

E

F

F

V

I

P

F

T

P

L

L

A

I

R

R

A

A

A

A

V

V

A

A

V

V

G

G

V

C

A

D

C

G

V

V

F

F

I

M

E

E

T

T

H

H

P

P

D

E

P

P

D

E

N

K

A

A

P

L

S

S

D
|
D

G

A

P

S

N

T

M

Q

V

L

P

P

V

L

K

S

E

Q

F

L

E

E

A

G

L

I

V

I

A

E

N

A

L

I

L

L

binding 1-deoxy-6-o-phosphono-1-[(phosphonomethyl)amino]-l-threo-hexitol: K45 (= K72), N47 (= N74), R48 (= R75), S49 (≠ T76), P100 (= P127), A101 (= A128), K123 (= K150), R153 (= R180), H184 (= H212), Q187 (= Q215), D226 (= D260)

1pckA Aquifex aeolicus kdo8ps in complex with z-methyl-pep (see paper)
49% identity, 87% coverage: 29:278/287 of query aligns to 1:245/258 of 1pckA

query
sites
1pckA

E

E

K

K

L

F

S

L

I

V

I

I

A

A

G

G

P

P

C

C

Q

A

M

I

E

E

S

S

R

E

Q

E

H

L

A

L

L

L

E

K

T

V

A

G

H

E

A

E

L

I

K

K

E

R

M

L

A

S

D

E

R

K

L

F

G

K

-

E

V

V

G

E

L

F

I

V

Y

F

K
|
K

T

S

S

S

Y

F

D

D

K
|
K

A

A

N
|
N

R

R

T

S

S

S

A

I

N

H

A

S

Q

F

R

R

G

G

I

H

G

G

L

L

K

E

D

Y

S

G

L

V

A

K

I

A

F

L

Q

R

E

K

I

V

R

K

E

E

V

E

T

F

G

G

L

L

P

K

T

I

L

T

T

T

D

D

V

I

H

H

E

E

T

S

S

W

H

Q

C

A

S

E

I

P

V

V

A

A

E

E

A

V

V

A

D

D

V

I

I

I

Q
|
Q

I

I

P
|
P

A
|
A

F

F

L

L

C

C

R

R

Q

Q

T

T

D

D

L

L

L

L

V

L

A

A

A

A

A

A

S

K

T

T

G

G

R

R

A

A

I

V

N

N

I

V

K
|
K

K

K

G

G

Q

Q

F

F

L

L

A

A

P

P

W

W

D

D

M

T

K

K

N

N

V

V

I

V

A

E

K

K

V

L

T

K

G

F

A

G

G

G

N

A

P

K

N

E

V

I

M

Y

A

L

C

T

E

E

R
|
R

G

G

A

T

S

T

F

F

G

G

Y

Y

N

N

T

N

L

L

V

V

S

V

D

D

M

F

R

R

A

S

L

L

P

P

I

I

M

M

K

K

E

Q

I

-

G

W

C

A

P

K

V

V

V

I

F

Y

D

D

A

A

T

T

H
|
H

S

S

V

V

Q

Q

Q

L

P

P

G

G

G

G

Q

L

G

-

T

-

S

-

S

-

G

-

G

G

Q

M

R

R

E

E

F

F

V

I

P

F

T

P

L

L

A

I

R

R

A

A

A

A

V

V

A

A

V

V

G

G

V

C

A

D

C

G

V

V

F

F

I

M

E

E

T

T

H

H

P

P

D

E

P

P

D

E

N

K

A

A

P

L

S

S

D

D

G

A

P

S

N

T

M

Q

V

L

P

P

V

L

K

S

E

Q

F

L

E

E

A

G

L

I

V

I

A

E

N

A

L

I

L

L

binding 2-(phosphonooxy)butanoic acid: K40 (= K67), K45 (= K72), N47 (= N74), Q98 (= Q125), P100 (= P127), A101 (= A128), K123 (= K150), R153 (= R180), H184 (= H212)

1jcxA Aquifex aeolicus kdo8p synthase in complex with api and cadmium (see paper)
49% identity, 87% coverage: 29:278/287 of query aligns to 1:242/255 of 1jcxA

query
sites
1jcxA

E

E

K

K

L

F

S

L

I

V

I

I

A

A

G

G

P

P

C

C

Q

A

M

I

E

E

S

S

R

E

Q

E

H

L

A

L

L

L

E

K

T

V

A

G

H

E

A

E

L

I

K

K

E

R

M

L

A

S

D

E

R

K

L

F

G

K

-

E

V

V

G

E

L

F

I

V

Y

F

K
|
K

T

S

S

S

Y

F

D

D

K
|
K

A

A

N
|
N

R
|
R

T
x
S

S

S

A

I

N

H

A

S

Q

F

R

R

G

G

I

H

G

G

L

L

K

E

D

Y

S

G

L

V

A

K

I

A

F

L

Q

R

E

K

I

V

R

K

E

E

V

E

T

F

G

G

L

L

P

K

T

I

L

T

T

T

D

D

V

I

H

H

E

E

T

S

S

W

H

Q

C

A

S

E

I

P

V

V

A

A

E

E

A

V

V

A

D

D

V

I

I

I

Q
|
Q

I

I

P
|
P

A
|
A

F

F

L

L

C

C

R

R

Q

Q

T

T

D

D

L

L

L

L

V

L

A

A

A

A

A

A

S

K

T

T

G

G

R

R

A

A

I

V

N

N

I

V

K
|
K

K

K

G

G

Q

Q

F

F

L

L

A

A

P

P

W

W

D

D

M

T

K

K

N

N

V

V

I

V

A

E

K

K

V

L

T

K

G

F

A

G

G

G

N

A

P

K

N

E

V

I

M

Y

A

L

C

T

E

E

R
|
R

G

G

A

T

S

T

F

F

G

G

Y

Y

N

N

T

N

L

L

V

V

S

V

D

D

M

F

R

R

A

S

L

L

P

P

I

I

M

M

K

K

E

Q

I

-

G

W

C

A

P

K

V

V

V

I

F

Y

D

D

A

A

T

T

H
|
H

S

S

V

V

Q
|
Q

Q

L

P

P

G

-

G

-

Q

-

G

-

T

-

S

-

S

-

G

-

G

G

Q

M

R

R

E

E

F

F

V

I

P

F

T

P

L

L

A

I

R

R

A

A

A

A

V

V

A

A

V

V

G

G

V

C

A

D

C

G

V

V

F
|
F

I

M

E
|
E

T

T

H

H

P

P

D

E

P

P

D

E

N

K

A

A

P

L

S

S

D

D

G

A

P

S

N

T

M

Q

V

L

P

P

V

L

K

S

E

Q

F

L

E

E

A

G

L

I

V

I

A

E

N

A

L

I

L

L

binding {[(2,2-dihydroxy-ethyl)-(2,3,4,5-tetrahydroxy-6-phosphonooxy-hexyl)-amino]-methyl}-phosphonic acid: K40 (= K67), K45 (= K72), N47 (= N74), R48 (= R75), S49 (≠ T76), Q98 (= Q125), P100 (= P127), A101 (= A128), K123 (= K150), R153 (= R180), H184 (= H212), Q187 (= Q215), F211 (= F247), E213 (= E249)

3undB Substrate-bound crystal structure of 2-dehydro-3-deoxyphosphooctonate aldolase from burkholderia pseudomallei (see paper)
45% identity, 92% coverage: 23:285/287 of query aligns to 12:276/284 of 3undB

query
sites
3undB

V

V

R

T

I

A

G

G

N

N

G

S

E

L

K

P

L

F

S

V

I

L

I

F

A

G

G

G

P

I

C
x
N

Q

V

M

L

E

E

S

S

R

L

Q

D

H

F

A

T

L

L

E

D

T

V

A

C

H

G

A

E

L

Y

K

V

E

A

M

V

A

T

D

R

R

K

L

L

G

G

V

I

G

P

L

F

I

V

Y

F

K
|
K

T

A

S

S

Y

F

D

D

K
|
K

A

A

N
|
N

R
|
R

T
x
S

S

S

A

I

N

H

A

S

Q

Y

R

R

G

G

I

V

G

G

L

L

K

D

D

E

S

G

L

L

A

K

I

I

F

F

Q

A

E

E

I

V

R

K

E

A

V

R

T

F

G

G

L

V

P

P

T

V

L

I

T

T

D

D

V

V

H

H

E

E

T

A

S

E

H

Q

C

A

S

A

I

P

V

V

A

A

E

E

A

I

V

A

D

D

V

V

I

L

Q
|
Q

I

V

P
|
P

A
|
A

F

F

L

L

C

A

R

R

Q

Q

T

T

D

D

L

L

L

V

V

V

A

A

A

I

A

A

S

K

T

A

G

G

R

K

A

P

I

V

N

N

I

V

K
|
K

K

K

G

P

Q

Q

F

F

L

M

A

S

P

P

W

T

D

Q

M

L

K

K

N

H

V

V

I

V

A

S

K

K

V

C

T

G

G

E

A

V

G

G

N

N

P

D

N

R

V

V

M

M

A

L

C

C

E

E

R
|
R

G

G

A

S

S

S

F

F

G

G

Y

Y

N

D

T

N

L

L

V

V

S

V

D

D

M

M

R

L

A

G

L

F

P

R

I

Q

M

M

K

A

E

E

I

T

-

T

-

G

G

G

C

C

P

P

V

V

V

I

F

F

D

D

A

V

T

T

H
|
H

S

S

V

L

Q
|
Q

Q

C

P
x
R

G

D

G

P

Q

L

G

G

T

D

S
x
A

S
|
S

G

G

G

G

Q

R

R

R

E

R

F

Q

V

V

P

L

T

D

L

L

A

A

R

R

A

A

A

G

V

I

A

A

V

V

G

G

V

I

A

A

C

G

V

L

F

F

I

L

E

E

T

A

H

H

P

P

D

D

P

P

D

D

N

R

A

A

P

R

S
x
C

D
|
D

G

G

P

P

N

S

M

A

V

L

P

P

V

L

K

H

E

Q

F

L

E

E

A

G

L

L

V

L

A

S

N

Q

L

M

L

K

R

A

Y

I

D

D

A

D

L

L

T

V

K

K

binding arabinose-5-phosphate: N27 (≠ C38), K61 (= K72), N63 (= N74), R64 (= R75), S65 (≠ T76), H203 (= H212), Q206 (= Q215), R208 (≠ P217), A214 (≠ S223), S215 (= S224), C250 (≠ S259), D251 (= D260)
binding 3-deoxy-8-O-phosphono-D-manno-oct-2-ulosonic acid: K56 (= K67), K61 (= K72), N63 (= N74), R64 (= R75), S65 (≠ T76), K139 (= K150), H203 (= H212), R208 (≠ P217), A214 (≠ S223), S215 (= S224), D251 (= D260)
binding phosphoenolpyruvate: K56 (= K67), Q114 (= Q125), P116 (= P127), A117 (= A128), K139 (= K150), R169 (= R180), H203 (= H212)

1g7vA Crystal structures of kdo8p synthase in its binary complexes with the mechanism-based inhibitor (see paper)
43% identity, 92% coverage: 23:286/287 of query aligns to 11:276/284 of 1g7vA

query
sites
1g7vA

V

I

R

N

I

V

G

A

N

N

G

D

E

L

K

P

L

F

S

V

I

L

I

F

A

G

G

G

P

M

C
x
N

Q

V

M

L

E

E

S

S

R

R

Q

D

H

L

A

A

L

M

E

R

T

I

A

C

H

E

A

H

L

Y

K

V

E

T

M

V

A

T

D

Q

R

K

L

L

G

G

V

I

G

P

L

Y

I

V

Y

F

K

K

T

A

S

S

Y

F

D

D

K
|
K

A

A

N
|
N

R
|
R

T

S

S

S

A

I

N

H

A

S

Q

Y

R

R

G

G

I

P

G

G

L

L

K

E

D

E

S

G

L

M

A

K

I

I

F

F

Q

Q

E

E

I

L

R

K

E

Q

V

T

T

F

G

G

L

V

P

K

T

I

L

I

T

T

D

D

V

V

H

H

E

E

T

P

S

S

H

Q

C

A

S

Q

I

P

V

V

A

A

E

D

A

V

V

V

D

D

V

V

I

I

Q

Q

I

L

P

P

A

A

F
|
F

L

L

C

A

R

R

Q

Q

T

T

D

D

L

L

L

V

V

E

A

A

A

M

A

A

S

K

T

T

G

G

R

A

A

V

I

I

N

N

I

V

K

K

K

K

G

P

Q

Q

F

F

L

V

A

S

P

P

W

G

D

Q

M

M

K

G

N

N

V

I

I

V

A

D

K

K

V

F

T

K

G

E

A

G

G

G

N

N

P

E

N

K

V

V

M

I

A

L

C

C

E

D

R
|
R

G

G

A

A

S

N

F

F

G

G

Y

Y

N

D

T

N

L

L

V

V

S

V

D

D

M

M

R

L

A

G

L

F

P

S

I

I

M

M

K

K

E

K

I

V

-

S

-

G

G

N

C

S

P

P

V

V

V

I

F

F

D

D

A

V

T

T

H
|
H

S

A

V

L

Q
|
Q

Q

C

P

R

G

D

G

P

Q

F

G

G

T

A

S

A

S

S

G

G

G

G

Q

R

R

R

E

A

F

Q

V

V

P

A

T

E

L

L

A

A

R

R

A

A

A

G

V

M

A

A

V

V

G

G

V

L

A

A

C

G

V

L

F

F

I

I

E

E

T

A

H

H

P

P

D

D

P

P

D

E

N

H

A
|
A

P

K

S

C

D
|
D

G
|
G

P
|
P

N

S

M

A

V

L

P

P

V

L

K

A

E

K

F

L

E

E

A

P

L

F

V

L

A

K

N

Q

L

M

L

K

R

A

Y

I

D

D

A

D

L

L

T

V

K

K

A

G

active site: N26 (≠ C38), K60 (= K72), D250 (= D260)
binding {[(2,2-dihydroxy-ethyl)-(2,3,4,5-tetrahydroxy-6-phosphonooxy-hexyl)-amino]-methyl}-phosphonic acid: N62 (= N74), R63 (= R75), F117 (= F129), R168 (= R180), H202 (= H212), Q205 (= Q215), A247 (= A257), G251 (= G261), P252 (= P262)

1g7uA Crystal structures of kdo8p synthase in its binary complex with substrate phosphoenol pyruvate (see paper)
43% identity, 92% coverage: 23:285/287 of query aligns to 11:275/284 of 1g7uA

query
sites
1g7uA

V

I

R

N

I

V

G

A

N

N

G

D

E

L

K

P

L

F

S

V

I

L

I

F

A

G

G

G

P

M

C

N

Q

V

M

L

E

E

S

S

R

R

Q

D

H

L

A

A

L

M

E

R

T

I

A

C

H

E

A

H

L

Y

K

V

E

T

M

V

A

T

D

Q

R

K

L

L

G

G

V

I

G

P

L

Y

I

V

Y

F

K

K

T

A

S

S

Y

F

D

D

K

K

A

A

N

N

R

R

T

S

S

S

A

I

N

H

A

S

Q

Y

R

R

G

G

I

P

G

G

L

L

K

E

D

E

S

G

L

M

A

K

I

I

F

F

Q

Q

E

E

I

L

R

K

E

Q

V

T

T

F

G

G

L

V

P

K

T

I

L

I

T

T

D

D

V

V

H

H

E

E

T

P

S

S

H

Q

C

A

S

Q

I

P

V

V

A

A

E

D

A

V

V

V

D

D

V

V

I

I

Q

Q

I

L

P

P

A

A

F
|
F

L

L

C

A

R

R

Q

Q

T

T

D

D

L

L

L

V

V

E

A

A

A

M

A

A

S

K

T

T

G

G

R

A

A

V

I

I

N

N

I

V

K

K

K

K

G

P

Q

Q

F

F

L

V

A

S

P

P

W

G

D

Q

M

M

K

G

N

N

V

I

I

V

A

D

K

K

V

F

T

K

G

E

A

G

G

G

N

N

P

E

N

K

V

V

M

I

A

L

C

C

E

D

R
|
R

G

G

A

A

S

N

F

F

G

G

Y

Y

N

D

T

N

L

L

V

V

S

V

D

D

M

M

R

L

A

G

L

F

P

S

I

I

M

M

K

K

E

K

I

V

-

S

-

G

G

N

C

S

P

P

V

V

V

I

F

F

D

D

A

V

T

T

H
|
H

S

A

V

L

Q

Q

Q

C

P

R

G

D

G

P

Q

F

G

V

T

A

S

A

S

S

G

G

G

G

Q

R

R

R

E

A

F

Q

V

V

P

A

T

E

L

L

A

A

R

R

A

A

A

G

V

M

A

A

V

V

G

G

V

L

A

A

C

G

V

L

F

F

I

I

E

E

T

A

H

H

P

P

D

D

P

P

D

E

N

H

A

A

P

K

S

C

D

D

G

G

P

P

N

S

M

A

V

L

P

P

V

L

K

A

E

K

F

L

E

E

A

P

L

F

V

L

A

K

N

Q

L

M

L

K

R

A

Y

I

D

D

A

D

L

L

T

V

K

K

binding phosphoenolpyruvate: F117 (= F129), R168 (= R180), H202 (= H212)

1x6uA Kdo8p synthase in it's binary complex with the product kdo8p (see paper)
42% identity, 92% coverage: 23:285/287 of query aligns to 11:263/272 of 1x6uA

query
sites
1x6uA

V

I

R

N

I

V

G

A

N

N

G

D

E

L

K

P

L

F

S

V

I

L

I

F

A

G

G

G

P

M

C
x
N

Q

V

M

L

E

E

S

S

R

R

Q

D

H

L

A

A

L

M

E

R

T

I

A

C

H

E

A

H

L

Y

K

V

E

T

M

V

A

T

D

Q

R

K

L

L

G

G

V

I

G

P

L

Y

I

V

Y

F

K
|
K

T

A

S

S

Y

F

D

D

K
|
K

A

A

N
|
N

R

R

T

S

S

S

A

I

N

H

A

S

Q

Y

R

R

G

G

I

P

G

G

L

L

K

E

D

E

S

G

L

M

A

K

I

I

F

F

Q

Q

E

E

I

L

R

K

E

Q

V

T

T

F

G

G

L

V

P

K

T

I

L

I

T

T

D

D

V

V

H

H

E

E

T

P

S

S

H

Q

C

A

S

Q

I

P

V

V

A

A

E

D

A

V

V

V

D

D

V

V

I

I

Q
|
Q

I

L

P

P

A

A

F
|
F

L

L

C

A

R

R

Q

Q

T

T

D

D

L

L

L

V

V

E

A

A

A

M

A

A

S

K

T

T

G

G

R

A

A

V

I

I

N

N

I

V

K
|
K

K

K

G

P

Q

Q

F

F

L

V

A

S

P

P

W

G

D

Q

M

M

K

G

N

N

V

I

I

V

A

D

K

K

V

F

T

K

G

E

A

G

G

G

N

N

P

E

N

K

V

V

M

I

A

L

C

C

E

D

R

R

G

G

A

A

S

N

F

F

G

G

Y

Y

N

D

T

N

L

L

V

V

S

V

D

D

M

M

R

L

A

G

L

F

P

S

I

I

M

M

K

K

E

K

I

V

-

S

-

G

G

N

C

S

P

P

V

V

V

I

F

F

D
|
D

A

V

T

T

H
|
H

S

A

V

L

Q

-

Q

-

P

-

G

-

G

-

Q

-

G

-

T

-

S

-

S

-

G

-

G

-

Q

Q

R

R

E

A

F

Q

V

V

P

A

T

E

L

L

A

A

R

R

A

A

A

G

V

M

A

A

V

V

G

G

V

L

A

A

C

G

V

L

F
|
F

I

I

E
|
E

T

A

H

H

P

P

D

D

P

P

D

E

N

H

A

A

P

K

S

C

D
|
D

G

G

P
|
P

N

S

M

A

V

L

P

P

V

L

K

A

E

K

F

L

E

E

A

P

L

F

V

L

A

K

N

Q

L

M

L

K

R

A

Y

I

D

D

A

D

L

L

T

V

K

K

active site: N26 (≠ C38), K60 (= K72), D238 (= D260)
binding 3-deoxy-8-O-phosphono-alpha-D-manno-oct-2-ulopyranosonic acid: N26 (≠ C38), K55 (= K67), N62 (= N74), Q113 (= Q125), F117 (= F129), K138 (= K150), D199 (= D209), H202 (= H212), F225 (= F247), E227 (= E249), P240 (= P262)

1phwA Crystal structure of kdo8p synthase in its binary complex with substrate analog 1-deoxy-a5p (see paper)
42% identity, 92% coverage: 23:285/287 of query aligns to 11:263/272 of 1phwA

query
sites
1phwA

V

I

R

N

I

V

G

A

N

N

G

D

E

L

K

P

L

F

S

V

I

L

I

F

A

G

G

G

P

M

C
x
N

Q

V

M

L

E

E

S

S

R

R

Q

D

H

L

A

A

L

M

E

R

T

I

A

C

H

E

A

H

L

Y

K

V

E

T

M

V

A

T

D

Q

R

K

L

L

G

G

V

I

G

P

L

Y

I

V

Y

F

K

K

T

A

S

S

Y

F

D

D

K
|
K

A

A

N

N

R

R

T

S

S

S

A

I

N

H

A

S

Q

Y

R

R

G

G

I

P

G

G

L

L

K

E

D

E

S

G

L

M

A

K

I

I

F

F

Q

Q

E

E

I

L

R

K

E

Q

V

T

T

F

G

G

L

V

P

K

T

I

L

I

T

T

D

D

V

V

H

H

E

E

T

P

S

S

H

Q

C

A

S

Q

I

P

V

V

A

A

E

D

A

V

V

V

D

D

V

V

I

I

Q

Q

I

L

P
|
P

A
|
A

F
|
F

L

L

C

A

R

R

Q

Q

T

T

D

D

L

L

L

V

V

E

A

A

A

M

A

A

S

K

T

T

G

G

R

A

A

V

I

I

N

N

I

V

K
|
K

K

K

G

P

Q

Q

F

F

L

V

A

S

P

P

W

G

D

Q

M

M

K

G

N

N

V

I

I

V

A

D

K

K

V

F

T

K

G

E

A

G

G

G

N

N

P

E

N

K

V

V

M

I

A

L

C

C

E

D

R
|
R

G

G

A

A

S

N

F

F

G

G

Y

Y

N

D

T

N

L

L

V

V

S

V

D

D

M

M

R

L

A

G

L

F

P

S

I

I

M

M

K

K

E

K

I

V

-

S

-

G

G

N

C

S

P

P

V

V

V

I

F

F

D

D

A

V

T

T

H
|
H

S

A

V

L

Q

-

Q

-

P

-

G

-

G

-

Q

-

G

-

T

-

S

-

S

-

G

-

G

-

Q

Q

R

R

E

A

F

Q

V

V

P

A

T

E

L

L

A

A

R

R

A

A

A

G

V

M

A

A

V

V

G

G

V

L

A

A

C

G

V

L

F

F

I

I

E

E

T

A

H

H

P

P

D

D

P

P

D

E

N

H

A

A

P

K

S

C

D
|
D

G

G

P

P

N

S

M

A

V

L

P

P

V

L

K

A

E

K

F

L

E

E

A

P

L

F

V

L

A

K

N

Q

L

M

L

K

R

A

Y

I

D

D

A

D

L

L

T

V

K

K

active site: N26 (≠ C38), K60 (= K72), D238 (= D260)
binding any 5'-monophosphate nucleotide: P115 (= P127), A116 (= A128), F117 (= F129), K138 (= K150), R168 (= R180), H202 (= H212)

1phqA Crystal structure of kdo8p synthase in its binary complex with substrate analog e-fpep
42% identity, 92% coverage: 23:285/287 of query aligns to 11:263/272 of 1phqA

query
sites
1phqA

V

I

R

N

I

V

G

A

N

N

G

D

E

L

K

P

L

F

S

V

I

L

I

F

A

G

G

G

P

M

C
x
N

Q

V

M

L

E

E

S

S

R

R

Q

D

H

L

A

A

L

M

E

R

T

I

A

C

H

E

A

H

L

Y

K

V

E

T

M

V

A

T

D

Q

R

K

L

L

G

G

V

I

G

P

L

Y

I

V

Y

F

K

K

T

A

S

S

Y

F

D

D

K
|
K

A

A

N

N

R

R

T

S

S

S

A

I

N

H

A

S

Q

Y

R

R

G

G

I

P

G

G

L

L

K

E

D

E

S

G

L

M

A

K

I

I

F

F

Q

Q

E

E

I

L

R

K

E

Q

V

T

T

F

G

G

L

V

P

K

T

I

L

I

T

T

D

D

V

V

H

H

E

E

T

P

S

S

H

Q

C

A

S

Q

I

P

V

V

A

A

E

D

A

V

V

V

D

D

V

V

I

I

Q

Q

I

L

P
|
P

A
|
A

F
|
F

L

L

C

A

R

R

Q

Q

T

T

D

D

L

L

L

V

V

E

A

A

A

M

A

A

S

K

T

T

G

G

R

A

A

V

I

I

N

N

I

V

K

K

K

K

G

P

Q

Q

F

F

L

V

A

S

P

P

W

G

D

Q

M

M

K

G

N

N

V

I

I

V

A

D

K

K

V

F

T

K

G

E

A

G

G

G

N

N

P

E

N

K

V

V

M

I

A

L

C

C

E

D

R
|
R

G

G

A

A

S

N

F

F

G

G

Y

Y

N

D

T

N

L

L

V

V

S

V

D

D

M

M

R

L

A

G

L

F

P

S

I

I

M

M

K

K

E

K

I

V

-

S

-

G

G

N

C

S

P

P

V

V

V

I

F

F

D

D

A

V

T

T

H
|
H

S

A

V

L

Q

-

Q

-

P

-

G

-

G

-

Q

-

G

-

T

-

S

-

S

-

G

-

G

-

Q

Q

R

R

E

A

F

Q

V

V

P

A

T

E

L

L

A

A

R

R

A

A

A

G

V

M

A

A

V

V

G

G

V

L

A

A

C

G

V

L

F

F

I

I

E

E

T

A

H

H

P

P

D

D

P

P

D

E

N

H

A

A

P

K

S

C

D
|
D

G

G

P

P

N

S

M

A

V

L

P

P

V

L

K

A

E

K

F

L

E

E

A

P

L

F

V

L

A

K

N

Q

L

M

L

K

R

A

Y

I

D

D

A

D

L

L

T

V

K

K

active site: N26 (≠ C38), K60 (= K72), D238 (= D260)
binding 3-fluoro-2-(phosphonooxy)propanoic acid: P115 (= P127), A116 (= A128), F117 (= F129), R168 (= R180), H202 (= H212)

1gg0A Crystal structure analysis of kdop synthase at 3.0 a (see paper)
42% identity, 92% coverage: 23:285/287 of query aligns to 11:266/275 of 1gg0A

query
sites
1gg0A

V

I

R

N

I

V

G

A

N

N

G

D

E

L

K

P

L

F

S

V

I

L

I

F

A

G

G

G

P

M

C
x
N

Q

V

M

L

E

E

S

S

R

R

Q

D

H

L

A

A

L

M

E

R

T

I

A

C

H

E

A

H

L

Y

K

V

E

T

M

V

A

T

D

Q

R

K

L

L

G

G

V

I

G

P

L

Y

I

V

Y

F

K

K

T

A

S

S

Y

F

D

D

K
|
K

A

A

N

N

R

R

T

S

S

S

A

I

N

H

A

S

Q

Y

R

R

G

G

I

P

G

G

L

L

K

E

D

E

S

G

L

M

A

K

I

I

F

F

Q

Q

E

E

I

L

R

K

E

Q

V

T

T

F

G

G

L

V

P

K

T

I

L

I

T

T

D

D

V

V

H

H

E

E

T

P

S

S

H

Q

C

A

S

Q

I

P

V

V

A

A

E

D

A

V

V

V

D

D

V

V

I

I

Q

Q

I

L

P

P

A
|
A

F

F

L

L

C

A

R

R

Q

Q

T

T

D

D

L

L

L

V

V

E

A

A

A

M

A

A

S

K

T

T

G

G

R

A

A

V

I

I

N

N

I

V

K
|
K

K

K

G

P

Q

Q

F

F

L

V

A

S

P

P

W

G

D

Q

M

M

K

G

N

N

V

I

I

V

A

D

K

K

V

F

T

K

G

E

A

G

G

G

N

N

P

E

N

K

V

V

M

I

A

L

C

C

E

D

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K

active site: N26 (≠ C38), K60 (= K72), D241 (= D260)
binding phosphate ion: A116 (= A128), K138 (= K150), R168 (= R180)

4z1dA Structure of pep and zinc bound kdo8ps from h.Pylori (see paper)
45% identity, 87% coverage: 33:283/287 of query aligns to 4:255/256 of 4z1dA

query
sites
4z1dA

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binding phosphoenolpyruvate: K38 (= K67), Q96 (= Q125), P98 (= P127), A99 (= A128), K121 (= K150), R164 (= R180), H195 (= H212)
binding zinc ion: C9 (= C38), H195 (= H212), E221 (= E249), D232 (= D260)

Query Sequence

>CCNA_01496 FitnessBrowser__Caulo:CCNA_01496
MDQPKAVAPNAVVEIKTPTGAPVRIGNGEKLSIIAGPCQMESRQHALETAHALKEMADRL
GVGLIYKTSYDKANRTSANAQRGIGLKDSLAIFQEIREVTGLPTLTDVHETSHCSIVAEA
VDVIQIPAFLCRQTDLLVAAASTGRAINIKKGQFLAPWDMKNVIAKVTGAGNPNVMACER
GASFGYNTLVSDMRALPIMKEIGCPVVFDATHSVQQPGGQGTSSGGQREFVPTLARAAVA
VGVACVFIETHPDPDNAPSDGPNMVPVKEFEALVANLLRYDALTKAA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory