SitesBLAST

Comparing CCNA_01576 FitnessBrowser__Caulo:CCNA_01576 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Found 15 hits to proteins with known functional sites

P0AA76 D-galactonate transporter; D-galactonate/H(+) symporter from Escherichia coli (strain K12) (see paper)
30% identity, 98% coverage: 8:429/431 of query aligns to 1:429/430 of P0AA76

• Y29 (= Y39) binding
• D31 (= D41) mutation to N: Loss of galactonate transport activity.
• R32 (= R42) binding
• Y64 (= Y74) binding
• E118 (= E128) mutation to Q: Loss of galactonate transport activity.
• W358 (≠ Y357) binding

6e9nA E. Coli d-galactonate:proton symporter in the inward open form (see paper)
30% identity, 94% coverage: 22:427/431 of query aligns to 1:408/409 of 6e9nA

• binding D-gluconic acid: F111 (= F132), N359 (≠ G377)

6e9oA E. Coli d-galactonate:proton symporter mutant e133q in the outward substrate-bound form (see paper)
29% identity, 95% coverage: 19:427/431 of query aligns to 1:392/393 of 6e9oA

• binding D-galactonic acid: Y21 (= Y39), F114 (= F132), Q141 (≠ T159), Q228 (≠ K247), S320 (≠ A354), N343 (≠ G377)

Q9JI12 Vesicular glutamate transporter 2; VGluT2; Differentiation-associated BNPI; Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter; Solute carrier family 17 member 6 from Rattus norvegicus (Rat) (see 2 papers)
21% identity, 92% coverage: 1:397/431 of query aligns to 43:470/582 of Q9JI12

• R88 (= R42) mutation to A: Impairs synaptic transmission. Abolishes the chloride ion conductance.
• H128 (≠ V67) mutation to A: Greatly lowers L-glutamate transport.
• R184 (= R121) mutation R->A,E,K: Greatly lowers L-glutamate transport.
• E191 (= E128) mutation to A: Greatly lowers L-glutamate transport.; mutation E->D,Q: Lowers L-glutamate transport.
• R322 (≠ I250) mutation to A: Loss of L-glutamate release. Abolishes the chloride ion conductance.

Q03567 Uncharacterized transporter slc-17.2 from Caenorhabditis elegans (see paper)
25% identity, 77% coverage: 55:386/431 of query aligns to 75:422/493 of Q03567

Sites not aligning to the query:

• 69 modified: carbohydrate, N-linked (GlcNAc...) asparagine

7t3nA R184q/e191q mutant of rat vesicular glutamate transporter 2 (vglut2)
21% identity, 87% coverage: 24:397/431 of query aligns to 12:412/452 of 7t3nA

• binding (1s,3r)-1-aminocyclopentane-1,3-dicarboxylic acid: Y77 (= Y74), Y137 (≠ F132), Y165 (≠ N160), R264 (≠ I250), F268 (≠ W254), Y269 (≠ W255)
• binding (2R)-2-(methoxymethyl)-4-{[(25R)-spirost-5-en-3beta-yl]oxy}butyl 4-O-alpha-D-glucopyranosyl-beta-D-glucopyranoside: R12 (= R24), Y13 (≠ W25), E152 (= E147), G163 (= G158)

Q9NRA2 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Membrane glycoprotein HP59; Solute carrier family 17 member 5; Vesicular excitatory amino acid transporter; VEAT from Homo sapiens (Human) (see 8 papers)
25% identity, 77% coverage: 55:386/431 of query aligns to 100:439/495 of Q9NRA2

• K136 (≠ R91) to E: in SD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; completely devoid of L-aspartate and L-glutamate transporter activity, but retains appreciable H(+)-coupled sialic acid transporter activity; dbSNP:rs80338795
• H183 (≠ I136) to R: in ISSD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; abolishes H(+)-coupled sialic acid transporter activity; has normal L-aspartate and L-glutamate transporter activity; dbSNP:rs119491109
• LL 198:199 (≠ AT 151:152) mutation to AA: Localizes in vesicular structures mainly concentrated in the perinuclear region.
• IL 266:267 (≠ I- 218) mutation to LA: Localizes in vesicular structures mainly concentrated in the perinuclear region.
• SSLRN 268:272 (≠ --EQD 219:221) natural variant: Missing (in ISSD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; abolishes H(+)-coupled sialic acid transporter activity; has normal L-aspartate and L-glutamate transporter activity)
• G328 (= G277) to E: in ISSD; some patients may manifest a milder phenotype consistent with Salla disease; markedly decreases H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs386833996
• P334 (≠ I283) to R: in ISSD; does not affect intracellular localization, targeted to lysosomes; abolishes H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs119491110
• G371 (vs. gap) to V: in ISSD; abolishes H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs777862172

Sites not aligning to the query:

• 22:23 Dileucine internalization motif; LL→AA: Targeted to plasma membrane.; LL→GG: Targeted to plasma membrane; sialic acid uptake strongly activated at acidic pH.
• 39 R → C: in SD; frequent variant in Finland; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; completely devoid of L-aspartate and L-glutamate transport activity, but retains appreciable H(+)-coupled sialic acid and nitrate transporter activity; dbSNP:rs80338794

Q5Q0U0 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Solute carrier family 17 (Anion/sugar transporter), member 5; Vesicular excitatory amino acid transporter; VEAT from Rattus norvegicus (Rat) (see 2 papers)
24% identity, 65% coverage: 109:386/431 of query aligns to 156:439/495 of Q5Q0U0

• R168 (= R121) mutation to C: Abolishes H(+)-coupled sialic acid transporter activity.
• E171 (≠ L124) mutation to C: Decreases H(+)-coupled sialic acid transporter activity; when associated with C-175.
• G172 (= G125) mutation to C: Decreases protein levels and alters subcellular localization.
• E175 (= E128) mutation to C: Decreases H(+)-coupled sialic acid transporter activity; when associated with C-171.
• G176 (≠ A129) mutation to C: Decreases protein levels and alters subcellular localization.
• F179 (= F132) mutation to C: Decreases the affinity and transport rate for D-glucuronate. Does not affect H(+)-coupled sialic acid transporter activity.
• P180 (= P133) mutation to C: Decreases protein levels and alters subcellular localization.
• H183 (≠ I136) mutation to R: Abolishes H(+)-coupled sialic acid transporter activity.
• W186 (≠ V139) mutation to C: Abolishes H(+)-coupled sialic acid transporter activity.
• SSLKN 268:272 (≠ QDPAD 220:224) mutation Missing: Abolishes H(+)-coupled sialic acid transporter activity.
• P334 (≠ I283) mutation to R: Abolishes H(+)-coupled sialic acid transporter activity.
• G371 (vs. gap) mutation to V: Remains in the endoplasmic reticulum.

Sites not aligning to the query:

• 22:23 Dileucine internalization motif; LL→AA: Targeted to plasma membrane; sialic acid uptake strongly activated at acidic pH.
• 39 R→C: Markedly decreases H(+)-coupled sialic acid transporter activity.
• 136 K→E: Markedly decreases H(+)-coupled sialic acid transporter activity.

Q5EXK5 3-hydroxybenzoate transporter MhbT from Klebsiella oxytoca (see paper)
22% identity, 96% coverage: 17:431/431 of query aligns to 13:444/452 of Q5EXK5

• D82 (= D86) mutation to A: Loss of activity.
• V311 (≠ W296) mutation to W: Loss of activity.
• D314 (≠ S299) mutation to A: Loss of activity.

Q8BN82 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Solute carrier family 17 member 5; Vesicular excitatory amino acid transporter; VEAT from Mus musculus (Mouse) (see paper)
23% identity, 65% coverage: 109:386/431 of query aligns to 156:439/495 of Q8BN82

• H183 (≠ I136) mutation to R: Abolishes sialic acid transporter activity. Does not affect L-aspartate and L-glutamate transporter activity.

Sites not aligning to the query:

• 39 R→C: Completely abolishes L-aspartate and L-glutamate transporter activity. Retains appreciable H(+)-coupled sialic acid transporter activity.

Q9GQQ0 Protein spinster; Protein benchwarmer; Protein diphthong from Drosophila melanogaster (Fruit fly) (see paper)
26% identity, 44% coverage: 11:200/431 of query aligns to 98:290/605 of Q9GQQ0

• E217 (= E128) mutation to K: In bnch(N); leads to storage in yolk spheres during oogenesis and results in widespread accumulation of enlarged lysosomal and late endosomal inclusions.

Q51955 4-hydroxybenzoate transporter PcaK from Pseudomonas putida (Arthrobacter siderocapsulatus) (see 2 papers)
23% identity, 42% coverage: 6:187/431 of query aligns to 10:190/448 of Q51955

• D41 (≠ N38) mutation D->A,N: Abolishes 4-HBA transport.; mutation to E: Decrease in 4-HBA transport.
• D44 (= D41) mutation D->A,N: Abolishes 4-HBA transport.; mutation to E: Decrease in 4-HBA transport.
• G85 (= G82) mutation to V: Abolishes 4-HBA transport and chemotaxis.
• D89 (= D86) mutation to N: Abolishes 4-HBA transport and chemotaxis.
• G92 (= G89) mutation to A: Decrease in 4-HBA transport and chemotaxis.; mutation to C: No change in 4-HBA transport and chemotaxis.; mutation G->L,V: Abolishes 4-HBA transport and chemotaxis.; mutation to Q: Decrease in 4-HBA transport and strong decrease in chemotaxis.
• R124 (= R121) mutation to A: Abolishes 4-HBA transport.
• E144 (= E141) mutation to A: Strong decrease in 4-HBA transport.
• H183 (≠ Q180) mutation to A: Decrease in 4-HBA transport and chemotaxis.

Sites not aligning to the query:

• 323 D→N: Abolishes 4-HBA transport and chemotaxis.
• 328 H→A: Decrease in 4-HBA transport and chemotaxis.; H→R: Decrease in 4-HBA transport and loss of chemotaxis.
• 386 R→A: Strong decrease in 4-HBA transport.
• 398 R→A: Abolishes 4-HBA transport.
• 444 H→A: No change in 4-HBA transport and chemotaxis.

Q9Y2C5 Probable small intestine urate exporter; Solute carrier family 17 member 4 from Homo sapiens (Human) (see 2 papers)
21% identity, 45% coverage: 82:277/431 of query aligns to 129:331/497 of Q9Y2C5

Sites not aligning to the query:

• 66 modified: carbohydrate, N-linked (GlcNAc...) asparagine; N→A: Loss of glycosylation and significant reduction in the tranport of thyroid hormones T3 and T4; when associated with A-75 and A-90.
• 75 modified: carbohydrate, N-linked (GlcNAc...) asparagine; N→A: Loss of glycosylation and significant reduction in the tranport of thyroid hormones T3 and T4; when associated with A-66 and A-90.
• 90 modified: carbohydrate, N-linked (GlcNAc...) asparagine; N→A: Loss of glycosylation and significant reduction in the tranport of thyroid hormones T3 and T4; when associated with A-75 and A-90.
• 372 A → T: in dbSNP:rs11754288

Q9C0U9 Uncharacterized transporter PB1C11.03 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
23% identity, 60% coverage: 3:259/431 of query aligns to 78:335/570 of Q9C0U9